LOCUS       NZ_CP028545            10060 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain SCKP020143 plasmid
            p3_020143, complete sequence.
ACCESSION   NZ_CP028545
VERSION     NZ_CP028545.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07609091
            Assembly: GCF_002853435.3
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain SCKP020143
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 10060)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   4  (bases 1 to 10060)
  AUTHORS   Liu,L., Lu,Y., Lin,J., Yang,B., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 02:42:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,831
            CDSs (total)                      :: 5,708
            Genes (coding)                    :: 5,563
            CDSs (with protein)               :: 5,563
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 51 of 145
            Pseudo Genes (incomplete)         :: 95 of 145
            Pseudo Genes (internal stop)      :: 12 of 145
            Pseudo Genes (multiple problems)  :: 13 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028545.2.
            On Aug 18, 2021 this sequence version replaced NZ_CP028545.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SCKP020143"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="p3_020143"
                     /geo_loc_name="China: Zigong, Sichuan"
                     /collection_date="2017"
     gene            complement(join(9707..10060,1..1611))
                     /locus_tag="CLQ46_RS01415"
                     /old_locus_tag="CLQ46_01585"
     CDS             complement(join(9707..10060,1..1611))
                     /locus_tag="CLQ46_RS01415"
                     /old_locus_tag="CLQ46_01585"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3095..3244)
                     /locus_tag="CLQ46_RS01420"
                     /old_locus_tag="CLQ46_01595"
     CDS             complement(3095..3244)
                     /locus_tag="CLQ46_RS01420"
                     /old_locus_tag="CLQ46_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3329..3586)
                     /locus_tag="CLQ46_RS01425"
                     /old_locus_tag="CLQ46_01600"
     CDS             complement(3329..3586)
                     /locus_tag="CLQ46_RS01425"
                     /old_locus_tag="CLQ46_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3596..5281)
                     /locus_tag="CLQ46_RS01430"
                     /old_locus_tag="CLQ46_01605"
     CDS             complement(3596..5281)
                     /locus_tag="CLQ46_RS01430"
                     /old_locus_tag="CLQ46_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5608..5853
                     /locus_tag="CLQ46_RS01435"
                     /old_locus_tag="CLQ46_01610"
     CDS             5608..5853
                     /locus_tag="CLQ46_RS01435"
                     /old_locus_tag="CLQ46_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            6127..6498
                     /gene="tolA"
                     /locus_tag="CLQ46_RS01440"
                     /old_locus_tag="CLQ46_01615"
     CDS             6127..6498
                     /gene="tolA"
                     /locus_tag="CLQ46_RS01440"
                     /old_locus_tag="CLQ46_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            6495..6860
                     /locus_tag="CLQ46_RS29005"
     CDS             6495..6860
                     /locus_tag="CLQ46_RS29005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            7035..7313
                     /locus_tag="CLQ46_RS01450"
                     /old_locus_tag="CLQ46_01555"
     CDS             7035..7313
                     /locus_tag="CLQ46_RS01450"
                     /old_locus_tag="CLQ46_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            7301..7615
                     /locus_tag="CLQ46_RS01455"
                     /old_locus_tag="CLQ46_01560"
     CDS             7301..7615
                     /locus_tag="CLQ46_RS01455"
                     /old_locus_tag="CLQ46_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            7779..8207
                     /locus_tag="CLQ46_RS01460"
                     /old_locus_tag="CLQ46_01565"
     CDS             7779..8207
                     /locus_tag="CLQ46_RS01460"
                     /old_locus_tag="CLQ46_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            8233..8412
                     /locus_tag="CLQ46_RS01465"
                     /old_locus_tag="CLQ46_01570"
     CDS             8233..8412
                     /locus_tag="CLQ46_RS01465"
                     /old_locus_tag="CLQ46_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8439..8969)
                     /locus_tag="CLQ46_RS01470"
                     /old_locus_tag="CLQ46_01575"
     CDS             complement(8439..8969)
                     /locus_tag="CLQ46_RS01470"
                     /old_locus_tag="CLQ46_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8976..9707)
                     /gene="mobC"
                     /locus_tag="CLQ46_RS01475"
                     /old_locus_tag="CLQ46_01580"
     CDS             complement(8976..9707)
                     /gene="mobC"
                     /locus_tag="CLQ46_RS01475"
                     /old_locus_tag="CLQ46_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     BGC             1..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2414..2992
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8980..9707)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 ctcctggctg gccgcgcgat agaaccagcg gatcggcgtg ttatccagta ccgtggtttt
       61 cacaaaatca gcgggcagat cctgcccgca ctgaccgaag tcgcccagcg actggtgcgc
      121 gagcagcagg ttgcagtccc ggctgcgcag cgtccccagc gcgttcagca cgtacttcga
      181 cagcagatat ttaatctcat cgagcatgat gctcgcgtgc ggccacctcc ggaactcatc
      241 ccgcgcaatg atgatttgcg cgcagcgggc aaacagcatc ttctgcaccc ggatcaccgc
      301 ctcatcgtcc atcgagccga tgacgtacag gcagccgccg ccgttgatta tccccgccac
      361 atcgataccg ctgtccgtct gcaggacgct caggtcggcc acgttctcca gcttggtgat
      421 cagccccttc acgtcctttt tcagggcctc cggcaacgcg tacgccgccg ccagcacctg
      481 ctgaatgttt gccccctgcg ggaactgctc cgcaatcagc ttcgccgctt tctgctcgct
      541 gatgcggtag tggtccgcca cgtcgccggt ctcactcagg ttaaacccgg caaccagcag
      601 attcttcagg gcgtactggt cgagatcgcg gaacagattc agctgcgccg gtttccccgg
      661 ccgcagatcc agcagggtga agtccgggca gtgcgcccgg aagacgtgcg gcgcccacgc
      721 gtcccctttg gggtcgaagt acaccacagc gtccccgaaa tcgcgtacgc agcgaatgag
      781 cgcgttggtg gccatcgcgc tcttgccgct cccgggcagg ccgaggatct gaatattggt
      841 cttgcgccag gtatcacgag ggaccagcac cggctggccc tgctcatcct tacccagaaa
      901 caccgcctgc tgtttccggg cctgctccgt ggaaaaatcg gccagcgcgt taaccggtac
      961 cggctccccg aaatgcaggt ggcggacgtc tgtcatttct ccggtccgct ggctgctgcg
     1021 ccggctctcc tgattcagtt tcgcgttcac cgcctcgtca gcggccgcaa aatgaccatc
     1081 aagcagcagg aacgccagca gccgcgccag aaggacgccg gcgacaagcc ctccggcaca
     1141 ccaggctatc ccgaacaggt tagccggctg cagcaggtaa taatctgcgt tcgcatagag
     1201 gtccgggcgc aggacggttt ctccccagct ctcgataaac acggcgacaa ccagcccccc
     1261 cagaagggcc agaccggcag cgccggtcag ccagtacgcg tagttccaga catgtcgccg
     1321 tgccctgacc atccgcccgg gtgaatccag cgaggccagc accgaaaatg gcacagtccg
     1381 gttattatcg cgcttatagc gatcggcata ctcccgccgc gccttatggc tcagccccac
     1441 aaaacgcatt cggccggccc agagttcagc cagaccgaaa aacaggccga taaccagaaa
     1501 aaaaccggcc agactgtaat actcccggta cagccagctg acccacgggt aaatctgccg
     1561 ggccagctcc gggatatgcc actgccaggc aagcgaatcc gtattaaaca tgcaatctct
     1621 ctccttcaga cgacgggttt accggcgtga tgcgcagccc ccgctgcaca agccgcggac
     1681 atgccccaag gcatggcggc ggcggtcacg tccgtggtga ttgcgttcag ctttcaccgc
     1741 ggaggccagc ctctttccgc cccatgcgga aaaggcgcag ccccggagga acaacgagca
     1801 cgccccagcg tgtgagagga aaaggcggca ccccagtgcg gtcttttccg gtgttccggc
     1861 ccccgtcaga gttcgccata gcgagcggct gaccacccgc aaaatcagga gcagctttgc
     1921 tgcttcgtca ggtgcgtcca gcaccgtgga tttttgtggg tggtcaggcg tgagcctgac
     1981 gggggccggg tgaatttctg gcgtggccgg cgtccagccg gacagagcca gaaagagggg
     2041 aaaagaccgt gcgtcggtgg cgtccagcca gcgtaagcaa ggtcttttcc cctgaagtca
     2101 gccacgcccc agcggggatg acttgcattt ctccgcaagg agaaaaaagc aacagagata
     2161 ggttaacgta aaatgttcag cgaaaaaccg acatggttca cctatcctga taattgatcg
     2221 tcagggaata gaaagactta atcaggggac aatatcccac atcagcgcgt ccggtgaccg
     2281 tcttgcaggt gccggggaaa ttgcgcctcc tcgctcactc ggtcgctacg ctccgggcgt
     2341 gagactgcgg cgggcgctgc ggacacatac aaagttaccc acagattccg tggataagca
     2401 ggggactaac atgtgaggca aaacggcagg gccgcgccgg tggcgttttt ccataggctc
     2461 cgccctcctg ccagagttca cataaacaga cgctttttcg gtgcatctgt gggagccgtg
     2521 aggctcaacc atgaatctga cagtacgggc gaaacccgac aggacttaaa gatccccacc
     2581 gtttccggta ggtcgctccc tcttgcgctc tcctgttccg accctgccgt ttaccggata
     2641 cctgtccgcc gttctccctt gcgggaagtg tggcgctttc tcatagctca cacactggta
     2701 tctcggctcg gtgtaggtcg ttcgctccaa gctgggctgt aagcaagaac tccccgttca
     2761 gcccgactgc tgcgccttat ccggtaactg ttcacttgag tccaacccgg gaaagcacgc
     2821 aaaacgccac tggcagcagc cattggtaac tgggagttcg cagaggattt gtttagctaa
     2881 acacgcggtt gctcttgaag tgtgcgccaa agtccggcta cactggaagg acagatttgg
     2941 ttgctgtgct ctgcgaaagc cagttaccac ggttaagcag ttccccaact gacttaacct
     3001 tcgatcaaac cacctcccca ggtggttttt tcgtttacag ggcaaaagat tacgcgcaga
     3061 aaaaaaggat ctcaagaaga tcctttgatc ttttctactg aaccgcgatc cccgtcagtt
     3121 cagaggagga cgatggtgcc accgttccgc cctgaacatc ccggatataa tttgcctgac
     3181 atgcggccag aagaaagcca gcaacaatca atataaaaag aaaaatagtc tttacctttt
     3241 tcatatactt tccctgtatt taaagactcc ggcattcatc cctgaacacc ggaagtcagt
     3301 atttcaaatc atccttgata attttttgtt accagttacc gtcccgataa tcaaaagcta
     3361 tataacaatt atccacatca ggttttattt gatttttaaa aaatggctgt agtaaaggaa
     3421 cccattcttt cttcaaatca aactcaccat tattaatatt atcctttaaa ggcattccca
     3481 agctttcaat gacagaacca tcgtcaccaa agtctttaga atactcttcc cctttaaact
     3541 cttcagtttt cttatcaaac catgtcaaac gtaatttaag ccccatattt acctctcaaa
     3601 gatatttttt aatgttccgt tttggatctg gccctttaac ctgcttaccc gttttgggat
     3661 caaatgcgcc taggtgttcg ccatcactgg cacgataccc ttcaagctca ccgtgctggg
     3721 aatcccattc ataaatttta cgttttttat cgccatacca tcgggcccgc ttaccaccac
     3781 caccttgttt aggtgtttta ggatcacctc gctttaactc tccaagtccc tttatgtctt
     3841 ctgttttagg tactggatga taatcatggc cgtagtcttt agtccccttg cgaggcttgt
     3901 tcttttcctc atttaactta ttttctgcat ccttcgcttt tttctccttt tgtttccggc
     3961 tctccatcgc cccggtaagt gcagccttat ctctggataa ggattcattc attgcgttta
     4021 aagatgcctg agccgctttt aatttctcat tctgattctg aatatcctta tcaagatagc
     4081 gaacctgatc ctgccacccg gagccatatt catgaggcgg gttataagtc acaaaatcat
     4141 ctttatttgc ctctgcctct tttacctttt tatcggcttc ctgaacagca gcccttgcac
     4201 cggggagcgc cttctctgtc gatgcaatgc ggctgtttaa ggtcgcaata tttttatctt
     4261 cggcatccag ctcagctttc gctttatcat agtctctttt cagcccctct tccggatgag
     4321 cggcgtccca tgcctgctgg cgacgctttt cttcctcctg acgctgtttt acctgtgccg
     4381 gggtaagaac atctgtcacc gacacataaa ccggcttctg tccggtctct ttcgggaaac
     4441 gaataacggc ctcacgggag ttaccaccgg ctgtaaaacc agccggacgt gaatcacctt
     4501 tttcagcaac aatgcctttt ggcggggctt tcgctgtcgg aacacctttc ggtacgctca
     4561 cctgcagaga cgggagaccc ggaatcgaca cactgaatac ccccggacgt tttgtcggtt
     4621 tcgcatccac cacagggaca ctcattggcc gacctgcgac aaccgcaata tgctgccgct
     4681 catccttcac cacatccaca acccgttgtc tgacacgaac cgtcgcctgg tccagtggta
     4741 aagtacttgc cggagtctcc gtcaccgcat cggccggtaa tgacgtcata atttttgaca
     4801 tcattttcgg atcatctttt gcaatctcag aaggcagcac accgtagatc gcaatccccc
     4861 acagaccaaa cttaaacggc cccttcaggg cagccagcac atcagcaacg gcggccgaca
     4921 acgcatcgcc ggaaactgat aaagccagtc cttcagcccc cggagtagcc agggcaggca
     4981 gaccgaaggc catcgcagtg gccgaagact gtccgccacc agagctaccg ctatgattgc
     5041 cgctgttacc accattacca ccgggtttac tcccgccgtt accaaactcc cccggattat
     5101 agttatgaat atctccattc ggcgtatgcg tcgtacccca gcccggcccg ctgtttggat
     5161 ccgtaccacc acctgatgaa gggccacctt taccagacgt accgttcaca ttcccactgg
     5221 cctgaccacc attatgtccc agacctgaat tacccggacc tcgaccgtct ccaccactca
     5281 taccgttttc ctctttgaca tttaaaacaa taaattaaag ccaacatact gtacatatac
     5341 acagtataat tattacctta ctcctcccgt tttcaggtgt caatatatga gattagttct
     5401 catcttcagg cacaaaaaag cgcccggagc cacctgtaag cagatcgcgt cttttcccga
     5461 aaatttctca tggcaacaaa ttttattatt taagacattc atcacactta catttcagat
     5521 ggtatagaca catctgaaag ctaactggca tgaggagagg acctgcggtt aaactgttcg
     5581 ggaaacctga gacgcccgga ggccactatg acgcacctga aactgactcc cgctgaagaa
     5641 aagcgcctgg cggacgcctt tgtcgctcag gcactggcca tgggcctgaa agggaacaaa
     5701 acgctgccaa aggcggtaaa acaacaggtg cgggaaaggg tgcttcgtgc cattcaggac
     5761 gaacgtgaag ccaggcgggc gcaggaaatg cgtcgggaga aatacgaggc tacggaaacc
     5821 accttcacct ggcagccaca gcgccgccgg taaggggtat ttaccataac gtcgcattgc
     5881 ccgcaccgct cagacggtct gagggcgttt ccggcggcta cgggataaaa caggcaacaa
     5941 accacccgga aaaccgccgg aaacgcgccc ggtaaattgt aacccgctgc atgcctatgc
     6001 acgcaactgc atggcgggcc gtttggtgta aaaacgatac gataagccgt gtttatgtaa
     6061 atgaacgtgg ccgtaaagag tgacaaatat acatgtaaca ttttttactg acaggtcatc
     6121 aacccgatgc gtttaatacc ctttatcctg ctggcattgt gcagcccctt actcctgacg
     6181 gcctgtaagc attccaccac ccagccgccg aaaatttata ctctggataa gtcatctaaa
     6241 atcagtagct acgctgcggc catcaagacc gctgttgaaa tgcagatgtt tgaaccagag
     6301 gcctatgcgg gaaaaatgtg caccgtgcgg atatccatac aacgtgatgg cactctgaac
     6361 agcgccattg ctgaaggagg agatccgaaa ctctgtaaag cagcaatatc agccgttacc
     6421 cacgccaaaa ttccgccggc accggatgaa aatacctggc aaatattcaa aaacgcccct
     6481 ctggatttca gaccatgaaa gcttttactc tgacagtgct ggcggccatg ttcctcactc
     6541 cggtacatgc gataccacta aaacccgtaa ccacactgca gccaacctac ccggtacagg
     6601 ccgcagcaaa acgcatcgaa ggtcaggtga ccgccacgtt tgatattctg gacgatggac
     6661 gagtggacaa cgtgtcgctt accggctgtc accttttttt tcgggaaacg ctgacggcaa
     6721 tgaggcactg gctatatccc gcagggcacc cggcaatgcg acaggttata aattttgaat
     6781 tcagtctgaa aaatgacggc ttacaggatc cgcgaccagt caccacccaa aagctcaatg
     6841 atgatggttg ccgccagtaa caggtccttt aacccgcagg ctgcaatagt cctggatgat
     6901 gagtgagcct gtagcagccc tgattagtgg aagcgaagct gaaaaatggg cagcaaaagc
     6961 cgtatttctg gcaaatagcg ccgccacgga ttaaagtagt catgtgtaat acatatgact
     7021 acaaaggggc ttttatgtca tcagcaacat ccatcaggct cgatgaagaa cttaaggacc
     7081 ggctgaaaac gctggccgat gaccggcacc gctcagcgca cgcgctaatg ctggaggcaa
     7141 tcaccgaata catcgaccgg gaagagaagc gcagccagta cctgcgggac ggacaggccg
     7201 catggcagca ctatcaggaa acgggtctgc accttactgc ggaagaagcg gaagcctgga
     7261 taagtacctg gggaactgag aacgagcagg atgctccgcc atgccacagg taacgatttc
     7321 cgcgctggca cagcgcgatt tacaacgcct tcaggacttt ctcaaaacca aaaatcggct
     7381 ggcagccaga aaaggaggtg aggtgatcgt ccgggctatc cagcaactga aaacattgcc
     7441 agacattggc cgcccggtgc cgtttctgcc gctggaatat aaggaactgg tgatcgggtt
     7501 tggcgacagt ggctatgtga tgctctaccg ccacgaccgg gaaatggacc agattgtgat
     7561 cgtcaccgtc aggcatcaga aagaatccgg gtatccgggg gcagacagcc tctaataacc
     7621 gatgacgggg gcgcaattca ttgcgtggcc gtcgccggtt tgacaagcgc agcgcgtcag
     7681 aaaaccacat cctgaacgga aaaacgggta aaattcagcg atacatttac cgtgagaaat
     7741 tacacacctt tcacacccga taaaatcagg ggtatacagt ggcgaaaaca ttgctcaacc
     7801 tttcacaggc agcacaagcc gcgggcatta ccagaagaac gctatacaat catgtaaaac
     7861 agggaaaggt taccgtgtca cgtgacggga aaaacaaccc tgtcgtggat gtgtcagaac
     7921 tgatccgtgt atacggaaat gtgaatatcc ctgagaaaca aattcccggc atttcacacc
     7981 gtgaaaatac acaaaaaaat ttcccacagg aacagcttct ggcaatgcaa aaggaactgg
     8041 ccgatctccg gcaggctgtc accctgatgc tggaagataa aacatcccgg gaagaagaac
     8101 gccgccagca cgatgatgag cgccggaaac tccaggccga agtggacaga ttgacaacag
     8161 aactgacaca gaagaaaaaa cgcttctggt ccggctggtt tagctgagaa cttaccgaag
     8221 ggaatggaga acatgacgaa gcaggaaaaa actgccctca atatggctaa attcattaag
     8281 gatcagtcgc tgctgttgct ggaaaagctg aacgagctgg acatggatac cccggccgac
     8341 atgtgcgaac ggcttcacga ggatgcggaa acactctggc aggtgatgcg ggataagctc
     8401 ggggaagaat aacagcaccg gccatgccgg tgctggcgtt attttttgcc gaacggccac
     8461 caccggcggg cgggtttgtc ctccggcagt tcaggcaccg gcacgttaaa atagcggctg
     8521 ttatattccc gcatcagctc cgctttcagc ttctcgttca ccgcgctttc cccgtgccgc
     8581 tcgctgacca cctcaatcat cgcgttaatc tgctccggtg agcacttccc ggcatcaacc
     8641 acctgcacac cgttctcgat ggcgaaccgc tccacctttc gatagaggta atcacacagc
     8701 ttcattcctg tgggtttgtc ccgttcccat tgcggatttt caccggtgaa aaagtacgtg
     8761 gctttttcgt cgctgttgag ccgctcgtca tacatcccga tggatttcat catcgccaga
     8821 tgctgggccc ggtcgacaaa acagaaaaca tacgccccgg tctgccaggc gagcaccacc
     8881 atgtcttcca gatgctcgct ggttggcata atgcagcgct cgccgtcttt gtcttccacg
     8941 tcctcaataa ataccatgta cagcgccata atccctcact ccgttgtcat aaatttaaag
     9001 cacgcgaaca gcctctcccg gtccagctcc accgttttgc gggcctcgcc ggtctgtgga
     9061 ttcacgattt catccaccgc cagaatcgcc cgctgtaccc ggacaagcgt cggccggtca
     9121 gggcagatgt accagatttc atcgatgccg ttcgcccggc ggttgaacag atacccggac
     9181 accacttcct gataccgcct gcggctcttg acggtgcgct ctacctcaaa tgccaccgtt
     9241 ctgccgtcca gctccgccag cgcgtccggc actttcatcc ctttcagggc catacggtgc
     9301 aggtaccgcc agccggtggc cccccggccc tccatggaga ggcgcaggga ctgcacggcc
     9361 agccggtgcg gcagctgcgc cgccgacacc cttgacggct gaaacggaat aacgtccgtc
     9421 agcggctcgt cctcgtcgaa cgagaacgcc accccgtgcg gcgtcagccc ccacacgctg
     9481 acctgacggc cgtcaaccgg gtatttcgcc cggaccagca gccctttcgc caccagactg
     9541 tccaggaagc ggtaaaccgg ctgtaccgcc gcaatcccca gccgtcgcgc aaccagctcc
     9601 gccgtggaac acagctcttc ccgcagccag cgcagggtca gccgtgtttt ctccgcgttc
     9661 cgcgcctgcc gcttcgcgtg gctgacgttg tactgctcca gtgtcatcag taaagccccg
     9721 caaaatcatc atccgctttt ggtccgccgc cgtcggccgg aggcaggtac tccgccagcg
     9781 ggtccgtttt cgccagcacc gggaaggttt tcagcgaaat ttcccggcgc tcaacccgca
     9841 gcgggctgct gaacgccagc cgcgccacgc ccagcccggt catcaccgcg aacccgtcag
     9901 gcaggtgttg cagggtgttc acgtcaaaca gcggccgcgc ctccttctgg atgaagctgt
     9961 cgccgctgat gtgctccacg ttccccgcct cccggctggc ccggcggcgc tccacgtcca
    10021 cccggatctc cccggtctgg cccgccgccc actgcgcgga
//
