LOCUS       NZ_CP028539            10060 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain WCHKP2 plasmid p1_020002, complete
            sequence.
ACCESSION   NZ_CP028539
VERSION     NZ_CP028539.3
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06909160
            Assembly: GCF_002164835.4
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain SCKP020002
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 10060)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 09:04:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,681
            CDSs (total)                      :: 5,561
            Genes (coding)                    :: 5,433
            CDSs (with protein)               :: 5,433
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 43 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 16 of 128
            Pseudo Genes (multiple problems)  :: 15 of 128
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028539.3.
            On Aug 18, 2021 this sequence version replaced NZ_CP028539.2.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP2"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p1_020002"
                     /geo_loc_name="China: Yibin, Sichuan"
                     /collection_date="2016-08"
     gene            complement(join(8498..10060,1..123))
                     /locus_tag="CAY63_RS00005"
                     /old_locus_tag="CAY63_00010"
     CDS             complement(join(8498..10060,1..123))
                     /locus_tag="CAY63_RS00005"
                     /old_locus_tag="CAY63_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            450..695
                     /locus_tag="CAY63_RS00010"
                     /old_locus_tag="CAY63_00015"
     CDS             450..695
                     /locus_tag="CAY63_RS00010"
                     /old_locus_tag="CAY63_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            969..1340
                     /gene="tolA"
                     /locus_tag="CAY63_RS00015"
                     /old_locus_tag="CAY63_00020"
     CDS             969..1340
                     /gene="tolA"
                     /locus_tag="CAY63_RS00015"
                     /old_locus_tag="CAY63_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            1337..1702
                     /locus_tag="CAY63_RS28245"
     CDS             1337..1702
                     /locus_tag="CAY63_RS28245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            1877..2155
                     /locus_tag="CAY63_RS00025"
                     /old_locus_tag="CAY63_00030"
     CDS             1877..2155
                     /locus_tag="CAY63_RS00025"
                     /old_locus_tag="CAY63_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            2143..2457
                     /locus_tag="CAY63_RS00030"
                     /old_locus_tag="CAY63_00035"
     CDS             2143..2457
                     /locus_tag="CAY63_RS00030"
                     /old_locus_tag="CAY63_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2621..3049
                     /locus_tag="CAY63_RS00035"
                     /old_locus_tag="CAY63_00040"
     CDS             2621..3049
                     /locus_tag="CAY63_RS00035"
                     /old_locus_tag="CAY63_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            3075..3254
                     /locus_tag="CAY63_RS00040"
                     /old_locus_tag="CAY63_00045"
     CDS             3075..3254
                     /locus_tag="CAY63_RS00040"
                     /old_locus_tag="CAY63_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3281..3811)
                     /locus_tag="CAY63_RS00045"
                     /old_locus_tag="CAY63_00050"
     CDS             complement(3281..3811)
                     /locus_tag="CAY63_RS00045"
                     /old_locus_tag="CAY63_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3818..4549)
                     /gene="mobC"
                     /locus_tag="CAY63_RS00050"
                     /old_locus_tag="CAY63_00055"
     CDS             complement(3818..4549)
                     /gene="mobC"
                     /locus_tag="CAY63_RS00050"
                     /old_locus_tag="CAY63_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(4549..6513)
                     /locus_tag="CAY63_RS00055"
                     /old_locus_tag="CAY63_00060"
     CDS             complement(4549..6513)
                     /locus_tag="CAY63_RS00055"
                     /old_locus_tag="CAY63_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(7997..8146)
                     /locus_tag="CAY63_RS00060"
                     /old_locus_tag="CAY63_00070"
     CDS             complement(7997..8146)
                     /locus_tag="CAY63_RS00060"
                     /old_locus_tag="CAY63_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(8231..8488)
                     /locus_tag="CAY63_RS00065"
                     /old_locus_tag="CAY63_00005"
     CDS             complement(8231..8488)
                     /locus_tag="CAY63_RS00065"
                     /old_locus_tag="CAY63_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     BGC             3498..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7316..7894
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3822..4549)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 atccgtacca ccacctgatg aagggccacc tttaccagac gtaccgttca cattcccact
       61 ggcctgacca ccattatgtc ccagacctga attacccgga cctcgaccgt ctccaccact
      121 cataccgttt tcctctttga catttaaaac aataaattaa agccaacata ctgtacatat
      181 acacagtata attattacct tactcctccc gttttcaggt gtcaatatat gagattagtt
      241 ctcatcttca ggcacaaaaa agcgcccgga gccacctgta agcagatcgc gtcttttccc
      301 gaaaatttct catggcaaca aattttatta tttaagacat tcatcacact tacatttcag
      361 atggtataga cacatctgaa agctaactgg catgaggaga ggacctgcgg ttaaactgtt
      421 cgggaaacct gagacgcccg gaggccacta tgacgcacct gaaactgact cccgctgaag
      481 aaaagcgcct ggcggacgcc tttgtcgctc aggcactggc catgggcctg aaagggaaca
      541 aaacgctgcc aaaggcggta aaacaacagg tgcgggaaag ggtgcttcgt gccattcagg
      601 acgaacgtga agccaggcgg gcgcaggaaa tgcgtcggga gaaatacgag gctacggaaa
      661 ccaccttcac ctggcagcca cagcgccgcc ggtaaggggt atttaccata acgtcgcatt
      721 gcccgcaccg ctcagacggt ctgagggcgt ttccggcggc tacgggataa aacaggcaac
      781 aaaccacccg gaaaaccgcc ggaaacgcgc ccggtaaatt gtaacccgct gcatgcctat
      841 gcacgcaact gcatggcggg ccgtttggtg taaaaacgat acgataagcc gtgtttatgt
      901 aaatgaacgt ggccgtaaag agtgacaaat atacatgtaa cattttttac tgacaggtca
      961 tcaacccgat gcgtttaata ccctttatcc tgctggcatt gtgcagcccc ttactcctga
     1021 cggcctgtaa gcattccacc acccagccgc cgaaaattta tactctggat aagtcatcta
     1081 aaatcagtag ctacgctgcg gccatcaaga ccgctgttga aatgcagatg tttgaaccag
     1141 aggcctatgc gggaaaaatg tgcaccgtgc ggatatccat acaacgtgat ggcactctga
     1201 acagcgccat tgctgaagga ggagatccga aactctgtaa agcagcaata tcagccgtta
     1261 cccacgccaa aattccgccg gcaccggatg aaaatacctg gcaaatattc aaaaacgccc
     1321 ctctggattt cagaccatga aagcttttac tctgacagtg ctggcggcca tgttcctcac
     1381 tccggtacat gcgataccac taaaacccgt aaccacactg cagccaacct acccggtaca
     1441 ggccgcagca aaacgcatcg aaggtcaggt gaccgccacg tttgatattc tggacgatgg
     1501 acgagtggac aacgtgtcgc ttaccggctg tcaccttttt tttcgggaaa cgctgacggc
     1561 aatgaggcac tggctatatc ccgcagggca cccggcaatg cgacaggtta taaattttga
     1621 attcagtctg aaaaatgacg gcttacagga tccgcgacca gtcaccaccc aaaagctcaa
     1681 tgatgatggt tgccgccagt aacaggtcct ttaacccgca ggctgcaata gtcctggatg
     1741 atgagtgagc ctgtagcagc cctgattagt ggaagcgaag ctgaaaaatg ggcagcaaaa
     1801 gccgtatttc tggcaaatag cgccgccacg gattaaagta gtcatgtgta atacatatga
     1861 ctacaaaggg gcttttatgt catcagcaac atccatcagg ctcgatgaag aacttaagga
     1921 ccggctgaaa acgctggccg atgaccggca ccgctcagcg cacgcgctaa tgctggaggc
     1981 aatcaccgaa tacatcgacc gggaagagaa gcgcagccag tacctgcggg acggacaggc
     2041 cgcatggcag cactatcagg aaacgggtct gcaccttact gcggaagaag cggaagcctg
     2101 gataagtacc tggggaactg agaacgagca ggatgctccg ccatgccaca ggtaacgatt
     2161 tccgcgctgg cacagcgcga tttacaacgc cttcaggact ttctcaaaac caaaaatcgg
     2221 ctggcagcca gaaaaggagg tgaggtgatc gtccgggcta tccagcaact gaaaacattg
     2281 ccagacattg gccgcccggt gccgtttctg ccgctggaat ataaggaact ggtgatcggg
     2341 tttggcgaca gtggctatgt gatgctctac cgccacgacc gggaaatgga ccagattgtg
     2401 atcgtcaccg tcaggcatca gaaagaatcc gggtatccgg gggcagacag cctctaataa
     2461 ccgatgacgg gggcgcaatt cattgcgtgg ccgtcgccgg tttgacaagc gcagcgcgtc
     2521 agaaaaccac atcctgaacg gaaaaacggg taaaattcag cgatacattt accgtgagaa
     2581 attacacacc tttcacaccc gataaaatca ggggtataca gtggcgaaaa cattgctcaa
     2641 cctttcacag gcagcacaag ccgcgggcat taccagaaga acgctataca atcatgtaaa
     2701 acagggaaag gttaccgtgt cacgtgacgg gaaaaacaac cctgtcgtgg atgtgtcaga
     2761 actgatccgt gtatacggaa atgtgaatat ccctgagaaa caaattcccg gcatttcaca
     2821 ccgtgaaaat acacaaaaaa atttcccaca ggaacagctt ctggcaatgc aaaaggaact
     2881 ggccgatctc cggcaggctg tcaccctgat gctggaagat aaaacatccc gggaagaaga
     2941 acgccgccag cacgatgatg agcgccggaa actccaggcc gaagtggaca gattgacaac
     3001 agaactgaca cagaagaaaa aacgcttctg gtccggctgg tttagctgag aacttaccga
     3061 agggaatgga gaacatgacg aagcaggaaa aaactgccct caatatggct aaattcatta
     3121 aggatcagtc gctgctgttg ctggaaaagc tgaacgagct ggacatggat accccggccg
     3181 acatgtgcga acggcttcac gaggatgcgg aaacactctg gcaggtgatg cgggataagc
     3241 tcggggaaga ataacagcac cggccatgcc ggtgctggcg ttattttttg ccgaacggcc
     3301 accaccggcg ggcgggtttg tcctccggca gttcaggcac cggcacgtta aaatagcggc
     3361 tgttatattc ccgcatcagc tccgctttca gcttctcgtt caccgcgctt tccccgtgcc
     3421 gctcgctgac cacctcaatc atcgcgttaa tctgctccgg tgagcacttc ccggcatcaa
     3481 ccacctgcac accgttctcg atggcgaacc gctccacctt tcgatagagg taatcacaca
     3541 gcttcattcc tgtgggtttg tcccgttccc attgcggatt ttcaccggtg aaaaagtacg
     3601 tggctttttc gtcgctgttg agccgctcgt catacatccc gatggatttc atcatcgcca
     3661 gatgctgggc ccggtcgaca aaacagaaaa catacgcccc ggtctgccag gcgagcacca
     3721 ccatgtcttc cagatgctcg ctggttggca taatgcagcg ctcgccgtct ttgtcttcca
     3781 cgtcctcaat aaataccatg tacagcgcca taatccctca ctccgttgtc ataaatttaa
     3841 agcacgcgaa cagcctctcc cggtccagct ccaccgtttt gcgggcctcg ccggtctgtg
     3901 gattcacgat ttcatccacc gccagaatcg cccgctgtac ccggacaagc gtcggccggt
     3961 cagggcagat gtaccagatt tcatcgatgc cgttcgcccg gcggttgaac agatacccgg
     4021 acaccacttc ctgataccgc ctgcggctct tgacggtgcg ctctacctca aatgccaccg
     4081 ttctgccgtc cagctccgcc agcgcgtccg gcactttcat ccctttcagg gccatacggt
     4141 gcaggtaccg ccagccggtg gccccccggc cctccatgga gaggcgcagg gactgcacgg
     4201 ccagccggtg cggcagctgc gccgccgaca cccttgacgg ctgaaacgga ataacgtccg
     4261 tcagcggctc gtcctcgtcg aacgagaacg ccaccccgtg cggcgtcagc ccccacacgc
     4321 tgacctgacg gccgtcaacc gggtatttcg cccggaccag cagccctttc gccaccagac
     4381 tgtccaggaa gcggtaaacc ggctgtaccg ccgcaatccc cagccgtcgc gcaaccagct
     4441 ccgccgtgga acacagctct tcccgcagcc agcgcagggt cagccgtgtt ttctccgcgt
     4501 tccgcgcctg ccgcttcgcg tggctgacgt tgtactgctc cagtgtcatc agtaaagccc
     4561 cgcaaaatca tcatccgctt ttggtccgcc gccgtcggcc ggaggcaggt actccgccag
     4621 cgggtccgtt ttcgccagca ccgggaaggt tttcagcgaa atttcccggc gctcaacccg
     4681 cagcgggctg ctgaacgcca gccgcgccac gcccagcccg gtcatcaccg cgaacccgtc
     4741 aggcaggtgt tgcagggtgt tcacgtcaaa cagcggccgc gcctccttct ggatgaagct
     4801 gtcgccgctg atgtgctcca cgttccccgc ctcccggctg gcccggcggc gctccacgtc
     4861 cacccggatc tccccggtct ggcccgccgc ccactgcgcg gactcctggc tggccgcgcg
     4921 atagaaccag cggatcggcg tgttatccag taccgtggtt ttcacaaaat cagcgggcag
     4981 atcctgcccg cactgaccga agtcgcccag cgactggtgc gcgagcagca ggttgcagtc
     5041 ccggctgcgc agcgtcccca gcgcgttcag cacgtacttc gacagcagat atttaatctc
     5101 atcgagcatg atgctcgcgt gcggccacct ccggaactca tcccgcgcaa tgatgatttg
     5161 cgcgcagcgg gcaaacagca tcttctgcac ccggatcacc gcctcatcgt ccatcgagcc
     5221 gatgacgtac aggcagccgc cgccgttgat tatccccgcc acatcgatac cgctgtccgt
     5281 ctgcaggacg ctcaggtcgg ccacgttctc cagcttggtg atcagcccct tcacgtcctt
     5341 tttcagggcc tccggcaacg cgtacgccgc cgccagcacc tgctgaatgt ttgccccctg
     5401 cgggaactgc tccgcaatca gcttcgccgc tttctgctcg ctgatgcggt agtggtccgc
     5461 cacgtcgccg gtctcactca ggttaaaccc ggcaaccagc agattcttca gggcgtactg
     5521 gtcgagatcg cggaacagat tcagctgcgc cggtttcccc ggccgcagat ccagcagggt
     5581 gaagtccggg cagtgcgccc ggaagacgtg cggcgcccac gcgtcccctt tggggtcgaa
     5641 gtacaccaca gcgtccccga aatcgcgtac gcagcgaatg agcgcgttgg tggccatcgc
     5701 gctcttgccg ctcccgggca ggccgaggat ctgaatattg gtcttgcgcc aggtatcacg
     5761 agggaccagc accggctggc cctgctcatc cttacccaga aacaccgcct gctgtttccg
     5821 ggcctgctcc gtggaaaaat cggccagcgc gttaaccggt accggctccc cgaaatgcag
     5881 gtggcggacg tctgtcattt ctccggtccg ctggctgctg cgccggctct cctgattcag
     5941 tttcgcgttc accgcctcgt cagcggccgc aaaatgacca tcaagcagca ggaacgccag
     6001 cagccgcgcc agaaggacgc cggcgacaag ccctccggca caccaggcta tcccgaacag
     6061 gttagccggc tgcagcaggt aataatctgc gttcgcatag aggtccgggc gcaggacggt
     6121 ttctccccag ctctcgataa acacggcgac aaccagcccc cccagaaggg ccagaccggc
     6181 agcgccggtc agccagtacg cgtagttcca gacatgtcgc cgtgccctga ccatccgccc
     6241 gggtgaatcc agcgaggcca gcaccgaaaa tggcacagtc cggttattat cgcgcttata
     6301 gcgatcggca tactcccgcc gcgccttatg gctcagcccc acaaaacgca ttcggccggc
     6361 ccagagttca gccagaccga aaaacaggcc gataaccaga aaaaaaccgg ccagactgta
     6421 atactcccgg tacagccagc tgacccacgg gtaaatctgc cgggccagct ccgggatatg
     6481 ccactgccag gcaagcgaat ccgtattaaa catgcaatct ctctccttca gacgacgggt
     6541 ttaccggcgt gatgcgcagc ccccgctgca caagccgcgg acatgcccca aggcatggcg
     6601 gcggcggtca cgtccgtggt gattgcgttc agctttcacc gcggaggcca gcctctttcc
     6661 gccccatgcg gaaaaggcgc agccccggag gaacaacgag cacgccccag cgtgtgagag
     6721 gaaaaggcgg caccccagtg cggtcttttc cggtgttccg gcccccgtca gagttcgcca
     6781 tagcgagcgg ctgaccaccc gcaaaatcag gagcagcttt gctgcttcgt caggtgcgtc
     6841 cagcaccgtg gatttttgtg ggtggtcagg cgtgagcctg acgggggccg ggtgaatttc
     6901 tggcgtggcc ggcgtccagc cggacagagc cagaaagagg ggaaaagacc gtgcgtcggt
     6961 ggcgtccagc cagcgtaagc aaggtctttt cccctgaagt cagccacgcc ccagcgggga
     7021 tgacttgcat ttctccgcaa ggagaaaaaa gcaacagaga taggttaacg taaaatgttc
     7081 agcgaaaaac cgacatggtt cacctatcct gataattgat cgtcagggaa tagaaagact
     7141 taatcagggg acaatatccc acatcagcgc gtccggtgac cgtcttgcag gtgccgggga
     7201 aattgcgcct cctcgctcac tcggtcgcta cgctccgggc gtgagactgc ggcgggcgct
     7261 gcggacacat acaaagttac ccacagattc cgtggataag caggggacta acatgtgagg
     7321 caaaacggca gggccgcgcc ggtggcgttt ttccataggc tccgccctcc tgccagagtt
     7381 cacataaaca gacgcttttt cggtgcatct gtgggagccg tgaggctcaa ccatgaatct
     7441 gacagtacgg gcgaaacccg acaggactta aagatcccca ccgtttccgg taggtcgctc
     7501 cctcttgcgc tctcctgttc cgaccctgcc gtttaccgga tacctgtccg ccgttctccc
     7561 ttgcgggaag tgtggcgctt tctcatagct cacacactgg tatctcggct cggtgtaggt
     7621 cgttcgctcc aagctgggct gtaagcaaga actccccgtt cagcccgact gctgcgcctt
     7681 atccggtaac tgttcacttg agtccaaccc gggaaagcac gcaaaacgcc actggcagca
     7741 gccattggta actgggagtt cgcagaggat ttgtttagct aaacacgcgg ttgctcttga
     7801 agtgtgcgcc aaagtccggc tacactggaa ggacagattt ggttgctgtg ctctgcgaaa
     7861 gccagttacc acggttaagc agttccccaa ctgacttaac cttcgatcaa accacctccc
     7921 caggtggttt tttcgtttac agggcaaaag attacgcgca gaaaaaaagg atctcaagaa
     7981 gatcctttga tcttttctac tgaaccgcga tccccgtcag ttcagaggag gacgatggtg
     8041 ccaccgttcc gccctgaaca tcccggatat aatttgcctg acatgcggcc agaagaaagc
     8101 cagcaacaat caatataaaa agaaaaatag tctttacctt tttcatatac tttccctgta
     8161 tttaaagact ccggcattca tccctgaaca ccggaagtca gtatttcaaa tcatccttga
     8221 taattttttg ttaccagtta ccgtcccgat aatcaaaagc tatataacaa ttatccacat
     8281 caggttttat ttgattttta aaaaatggct gtagtaaagg aacccattct ttcttcaaat
     8341 caaactcacc attattaata ttatccttta aaggcattcc caagctttca atgacagaac
     8401 catcgtcacc aaagtcttta gaatactctt cccctttaaa ctcttcagtt ttcttatcaa
     8461 accatgtcaa acgtaattta agccccatat ttacctctca aagatatttt ttaatgttcc
     8521 gttttggatc tggcccttta acctgcttac ccgttttggg atcaaatgcg cctaggtgtt
     8581 cgccatcact ggcacgatac ccttcaagct caccgtgctg ggaatcccat tcataaattt
     8641 tacgtttttt atcgccatac catcgggccc gcttaccacc accaccttgt ttaggtgttt
     8701 taggatcacc tcgctttaac tctccaagtc cctttatgtc ttctgtttta ggtactggat
     8761 gataatcatg gccgtagtct ttagtcccct tgcgaggctt gttcttttcc tcatttaact
     8821 tattttctgc atccttcgct tttttctcct tttgtttccg gctctccatc gccccggtaa
     8881 gtgcagcctt atctctggat aaggattcat tcattgcgtt taaagatgcc tgagccgctt
     8941 ttaatttctc attctgattc tgaatatcct tatcaagata gcgaacctga tcctgccacc
     9001 cggagccata ttcatgaggc gggttataag tcacaaaatc atctttattt gcctctgcct
     9061 cttttacctt tttatcggct tcctgaacag cagcccttgc accggggagc gccttctctg
     9121 tcgatgcaat gcggctgttt aaggtcgcaa tatttttatc ttcggcatcc agctcagctt
     9181 tcgctttatc atagtctctt ttcagcccct cttccggatg agcggcgtcc catgcctgct
     9241 ggcgacgctt ttcttcctcc tgacgctgtt ttacctgtgc cggggtaaga acatctgtca
     9301 ccgacacata aaccggcttc tgtccggtct ctttcgggaa acgaataacg gcctcacggg
     9361 agttaccacc ggctgtaaaa ccagccggac gtgaatcacc tttttcagca acaatgcctt
     9421 ttggcggggc tttcgctgtc ggaacacctt tcggtacgct cacctgcaga gacgggagac
     9481 ccggaatcga cacactgaat acccccggac gttttgtcgg tttcgcatcc accacaggga
     9541 cactcattgg ccgacctgcg acaaccgcaa tatgctgccg ctcatccttc accacatcca
     9601 caacccgttg tctgacacga accgtcgcct ggtccagtgg taaagtactt gccggagtct
     9661 ccgtcaccgc atcggccggt aatgacgtca taatttttga catcattttc ggatcatctt
     9721 ttgcaatctc agaaggcagc acaccgtaga tcgcaatccc ccacagacca aacttaaacg
     9781 gccccttcag ggcagccagc acatcagcaa cggcggccga caacgcatcg ccggaaactg
     9841 ataaagccag tccttcagcc cccggagtag ccagggcagg cagaccgaag gccatcgcag
     9901 tggccgaaga ctgtccgcca ccagagctac cgctatgatt gccgctgtta ccaccattac
     9961 caccgggttt actcccgccg ttaccaaact cccccggatt atagttatga atatctccat
    10021 tcggcgtatg cgtcgtaccc cagcccggcc cgctgtttgg
//
