LOCUS       NZ_CP028467             1532 bp    DNA     circular CON 02-DEC-2023
DEFINITION  Escherichia coli strain WCHEC050613 plasmid p4_050613, complete
            sequence.
ACCESSION   NZ_CP028467
VERSION     NZ_CP028467.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06197014
            Assembly: GCF_001969285.3
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Zhao,F., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain WCHEC050613
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Zhao,F., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 1532)
  AUTHORS   Zhao,F., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/02/2023 11:20:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,030
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 66 of 260
            Pseudo Genes (incomplete)         :: 185 of 260
            Pseudo Genes (internal stop)      :: 45 of 260
            Pseudo Genes (multiple problems)  :: 35 of 260
            Pseudo Genes (short protein)      :: 4 of 260
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028467.2.
            On Aug 26, 2019 this sequence version replaced NZ_CP028467.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WCHEC050613"
                     /isolation_source="Sewage"
                     /db_xref="taxon:562"
                     /plasmid="p4_050613"
                     /geo_loc_name="China: Sichuan, Chengdu"
                     /collection_date="2015-11"
     gene            13..894
                     /locus_tag="DBA32_RS25325"
                     /old_locus_tag="DBA32_26860"
     CDS             13..894
                     /locus_tag="DBA32_RS25325"
                     /old_locus_tag="DBA32_26860"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071448089.1"
                     /translation="MATLKLYHNGLTAGIPPMKNNHERAKRGEVKGWSKSASRNNTKFL
                     RSVHVPDLTGAAVAFTLTIRDCPPSSEDWKILRENFIKRLRRAGMVRLHWLTEWQQRGV
                     PHLHGIAYFPDAYEPSTVIWNWIAVAEKYGCNMRSQHVVPITDMQGWFMYLAKHAGRSE
                     THYQRNPEFVPAGWAKTGRVWGYCGDWPIDEPVTLDASHKAFCALRRICRNWRLADARS
                     ANYGTVKDAEKKRRRRIISARRMLRCNKLGQSASHGVSEWIPLDVSLTAAYWCMASHPM
                     PEVDDSRYQIEI"
                     /name="hypothetical protein"
ORIGIN
        1 taaggaaaca acttggctac gctgaaatta tatcacaacg gtctgacggc tgggattccc
       61 cctatgaaga ataatcatga gcgcgccaag cgcggggaag ttaaaggctg gtctaaaagc
      121 gcctcccgca acaacaccaa attccttcgc tccgttcacg ttcccgatct cactggcgct
      181 gctgtggcgt ttacgctaac tattcgtgat tgcccgccat catctgagga ttggaagatc
      241 ctccgcgaga atttcattaa gcgccttcgc cgtgctggga tggtgcgcct tcactggctt
      301 acggaatggc aacagcgggg tgttcctcac cttcacggga tcgcctattt tcctgatgct
      361 tacgaaccct ccactgtaat ctggaattgg atcgccgttg ctgagaagta cggctgcaat
      421 atgcgctcgc agcatgttgt acctatcacg gatatgcagg gttggttcat gtacctggct
      481 aagcatgctg gccgctctga gactcactat cagcgcaatc ctgagtttgt gcccgcagga
      541 tgggctaaaa cgggccgtgt gtggggttac tgcggtgatt ggcccattga tgagcctgtg
      601 acgcttgatg cctctcacaa ggctttctgt gcgcttaggc gtatttgccg gaactggcgt
      661 cttgccgatg cccgttcagc caattacggc actgtgaagg acgcagaaaa gaagcgccgc
      721 cggcgtatta tctccgcccg cagaatgctc cgctgtaata agctcggtca gtccgcttcg
      781 cacggtgttt ctgagtggat ccctctcgat gtttccctta ccgctgcgta ctggtgtatg
      841 gccagtcacc ccatgccgga agtagacgac tcacgctatc agattgaaat ctgacagcgt
      901 tcgccgccgc cgaatcgtca actgctaaat cgggggatcc ctttagggtt ccgatttagt
      961 ggttgcacga ctgcaccccc ccataacctt gtattgggtg atggttcaca tggtgagcca
     1021 tcgccctgta aagccggttt taacccccct tcaaggcgtt gcctgtgggg taggtgcgca
     1081 agccctgccc tacaggtgat gccgtggggt gttcccgttt tgctcggtat gccttatttg
     1141 tcgatttttg tctaagtgag gtcatggggg atggtcgtgc tgctacggtg aggaacgggg
     1201 acgcccccgc agctctgcga ggaggcgctc gccgttctga atcgtagtcc actctgttta
     1261 atagtggact ctcgtagcaa aatgcaacaa aaggccggtc gtgcagtgta tgtaatactg
     1321 cacgttcgtc tcattctgat tctgttcgtt gagtgtttga gtgcttcgat ttttcagcgg
     1381 ttcaggtgct tcccttgatt agttgttaca taactgaccg cgaccccttc ctaactaatc
     1441 tttttgggtt tattgtttcc ctgtttttta ggcaaaaaat taccccgcaa ctgcgtcaac
     1501 agtccggggc ttggtaacca ctcgaatgtg tt
//
