LOCUS       NZ_CP028466             3398 bp    DNA     circular CON 02-DEC-2023
DEFINITION  Escherichia coli strain WCHEC050613 plasmid p3_050613, complete
            sequence.
ACCESSION   NZ_CP028466
VERSION     NZ_CP028466.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06197014
            Assembly: GCF_001969285.3
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Zhao,F., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain WCHEC050613
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Zhao,F., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/02/2023 11:20:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,030
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 66 of 260
            Pseudo Genes (incomplete)         :: 185 of 260
            Pseudo Genes (internal stop)      :: 45 of 260
            Pseudo Genes (multiple problems)  :: 35 of 260
            Pseudo Genes (short protein)      :: 4 of 260
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028466.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WCHEC050613"
                     /isolation_source="Sewage"
                     /db_xref="taxon:562"
                     /plasmid="p3_050613"
                     /geo_loc_name="China: Sichuan, Chengdu"
                     /collection_date="2015-11"
     gene            complement(363..512)
                     /locus_tag="DBA32_RS25475"
     CDS             complement(363..512)
                     /locus_tag="DBA32_RS25475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001442891.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            1758..2189
                     /locus_tag="DBA32_RS25310"
                     /old_locus_tag="DBA32_26845"
     CDS             1758..2189
                     /locus_tag="DBA32_RS25310"
                     /old_locus_tag="DBA32_26845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660103.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_071448170.1"
                     /translation="MKAKTEPLEKKWGERSMLMGWTAIPTSLLFLQAELSISPLGFNLL
                     MNLISHWWETTEHPYPSQESMAKRMGVSKRSIQREITTLVELGLLDKQPNKAGSKKFKG
                     RNTYDLSKLVQVLNEKTPDKISSFKRYKKNGESLDSELE"
                     /name="helix-turn-helix domain-containing protein"
     gene            2199..2651
                     /locus_tag="DBA32_RS25315"
                     /old_locus_tag="DBA32_26850"
     CDS             2199..2651
                     /locus_tag="DBA32_RS25315"
                     /old_locus_tag="DBA32_26850"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001141956.1"
                     /translation="MPTFTQSGTGKFDYWLIDGVKSFSKIPANTLPSITVDMPIRLQVG
                     NGYFGSTHITARHGKWLQRYQPDGCVATFIHKKLSTSGKILLLEEQDKIGLALRLNPDS
                     ALILKNIGDFFSVTTIYYKRSGLQGNEIGRYTGSSWATSPFIDRKR"
                     /name="hypothetical protein"
     gene            complement(3010..3378)
                     /locus_tag="DBA32_RS25320"
                     /old_locus_tag="DBA32_26855"
     CDS             complement(3010..3378)
                     /locus_tag="DBA32_RS25320"
                     /old_locus_tag="DBA32_26855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001273380.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNDFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     Typing          complement(506..720)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.041"
                     /coverage="100.0"
                     /evalue="1.57e-69"
     Typing          696..1183
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.688"
                     /coverage="93.0"
                     /evalue="0.0"
ORIGIN
        1 catttgtctc ataatagtcc aaaaataaga gtaaaacgca aaaaatgtct tacaattgtc
       61 ttatttttgt cctttccggt tcatgtatcc atgaaccgcc tgatgtggaa cgctttttat
      121 cgatctcatg cgctgccaaa gatagattca ttctttaaaa gtcaggttac attatccctt
      181 aaatggccat acaccatacg caaccgcgat tttcatcgca attgcatgaa ttaaaaggta
      241 aaaaccgaat ttcccgttta tttatgtaat acgcaacgta ttacaaccac ctgtaatcac
      301 atgaaaataa agaaataaga tgtaataccg gcgtattaca cgtacgcggg taagcgaaaa
      361 agctataacg cttcacggcc gacaggtggc ccaaaatcca ccggggcgtg caggttctct
      421 tcagtaacct gcgccattaa atcaaaaagg tcatattcgc cgggaagctg tgtatcagtc
      481 gaatccagtt gttcatcata ttcagatggc atggggtgat atccaatagc tgagaggttg
      541 ttagagggtt tcggggcgca gccctgaacc agtcacgtag cgctagcgga gtgtatactg
      601 gctgaagatg ttagatcgga gtggtaattc agagaagtta ttcatgttgc aagggaaaat
      661 gcggctatag ggcgtaagtg caaaatgcag gtaacggtga catgacgcct cctcgctcac
      721 tcggtcgcta cgctcctgcc gtgaggctgc ggcgggcgtt acctgctcac gaatgacaag
      781 ataaagagat ggcactcaag tcaggaaggg ccggaaaaag gatacggcgc tgccgttttt
      841 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggcggcg
      901 aaacccgaca ggactataaa gatcccaggc gtttccccct ggtagctccc tcgtgcgctc
      961 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca
     1021 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg
     1081 aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcaactt gagtccaacc
     1141 cggaaagaca cgacaaagcg ccactggcag cagccatagg taagaaggtg tacaagaaga
     1201 ttactagtgc agagttcttg aagtctgagc caaattacgg ctaaactgga aggacgaatt
     1261 tggtggcagc ggtcttgtac accctgttac cacggttcat aaacttgccg gtttaatgaa
     1321 ccttcgaaaa accaccttgc cgggtggttt tttcgtttct gagactacat ttttgcttac
     1381 cttcctgtca atcaagaaga taacaaacaa aaaaagctgc ccctggcagc tttttttatg
     1441 agtccgagtg gcgtacgtga tgtctgtgat cagattcatc atccctctct gtgagggagc
     1501 ccactcaagt ggctgcgaaa cgcagggcga tccactatca gcgcatgttg cctcgccatt
     1561 atatcgccat gacggacaaa tcgtcttctg taagactcat agcaagtatg gtgacaaaac
     1621 catatcaaaa aaaggcgaca aaatgtctat acatatgaat atacatatat aattataacc
     1681 aagaacactc taacatcatt aaataatgat gttagagtgt tcttggtgtc gctacaaatt
     1741 aaaaaacgag gttcgtaatg aaagcgaaga cagagccact tgaaaaaaag tggggagaac
     1801 gaagcatgct tatgggatgg acagcgatcc ctacatcgct gctttttcta caggcggaac
     1861 tatcaattag tcctttgggt ttcaatttgt tgatgaacct tatttctcac tggtgggaaa
     1921 ctacagagca cccttaccct tcacaagaat cgatggcaaa aagaatgggc gtttcgaaac
     1981 gttctatcca gagagaaatc acaactttgg tagagcttgg cttactcgat aaacagccga
     2041 ataaggccgg gagcaagaaa tttaaaggtc gaaacaccta tgacttaagc aagcttgttc
     2101 aagtccttaa tgaaaaaaca cctgataaaa taagcagttt caaacgttat aaaaaaaatg
     2161 gagaatcact agattcagag ctggagtaat cttagaaaat gccgacattc acgcaatctg
     2221 gaactgggaa gtttgattat tggcttatag atggagttaa gtctttttca aaaataccgg
     2281 ctaacacact gccatcaatc accgtagata tgccgatccg cttacaagtc ggaaatggtt
     2341 actttggctc aacacacata accgccaggc atggtaagtg gcttcaacgc tatcaaccag
     2401 atggttgtgt agcaacattc atccataaga agctcagcac cagtggaaag atcttactgt
     2461 tggaagagca agataaaata ggtttagctc tcagactgaa cccagactca gctctgatac
     2521 tgaaaaacat tggtgatttt ttctctgtaa caacaatcta ttacaaacga agtgggcttc
     2581 agggaaatga aataggaagg tataccggtt catcgtgggc gacatctcct tttatcgata
     2641 gaaagcgata accccaccaa gatttaccat aacatctctt gcgtgcaccg cctgagaggc
     2701 tctcagggcg tttctgtggc ctgagccata aaaagcccca tcttctcccc gaaaacccgc
     2761 cagaaacgcg ctgagagatt ttcagcccta tgcatgacta tgcaccggac taactcgcag
     2821 gcgattttgg cctgaattca ggccggatga cttaaaatct gcaccaaagc caacagaaaa
     2881 cggtgaacgg gtgcgcaatt tattgcgtgg ccgtgaggca ttgaccaagc cgtaaggcac
     2941 ggtaggtaat tgcaggtatc cgacaatttc gagtagagga tccactattt cactacttag
     3001 aattcaaaat cagaatcctt tcttcctgct aaggctatct ctgaatgact caaccactct
     3061 ttgagattct tcttctgctc gcatgaagtc attcccggac agaaaaaaat cattgtcagg
     3121 cccactggct tcagagaaat tcgtttctgc agcagacaca gaatccacat cagcaagaga
     3181 cggttcatct gtactttttc tcaatctgta aagagcctta tcaaacgcaa ccagtgagat
     3241 attcaggccg taacgttcat tcagcacagt cacaatctct tccctacgga taccattttt
     3301 cttccaggtt tctatatcgt tcttggaatc cctgacagct gcaatggctg ttcttggacc
     3361 agggttaaaa ttcgccatat accgcccata atgtctta
//
