LOCUS       NZ_CP028323            12894 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Escherichia coli O18:H1 strain CFSAN067215 plasmid p0.1229_3,
            complete sequence.
ACCESSION   NZ_CP028323
VERSION     NZ_CP028323.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08737532
            Assembly: GCF_004771135.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O18:H1
  ORGANISM  Escherichia coli O18:H1
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12894)
  AUTHORS   Hoffman,M., Sanchez,M., Keefer,A., Figler,H. and Dudley,E.
  TITLE     Whole genome sequencing of cultured foodborne pathogen
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12894)
  AUTHORS   Hoffman,M., Sanchez,M., Keefer,A., Figler,H. and Dudley,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2018) CFSAN-ORS-DM-MMSB, US Food and Drug
            Administration, 5100 Paint Branch Parkway, College Park, MD 20740,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 405.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004771135.1-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 16:17:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,154
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,788
            CDSs (with protein)               :: 4,788
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 241
            CDSs (without protein)            :: 241
            Pseudo Genes (ambiguous residues) :: 0 of 241
            Pseudo Genes (frameshifted)       :: 105 of 241
            Pseudo Genes (incomplete)         :: 148 of 241
            Pseudo Genes (internal stop)      :: 50 of 241
            Pseudo Genes (multiple problems)  :: 54 of 241
            Pseudo Genes (short protein)      :: 2 of 241
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028323.1.
            Bacteria and source DNA available from Bacterial culture available
            from Dwayne Roberson, FDA College Park Campus, 5001 Campus Drive,
            College Park, MD 20740.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12894
                     /organism="Escherichia coli O18:H1"
                     /mol_type="genomic DNA"
                     /strain="CFSAN067215"
                     /host="Homo sapiens"
                     /db_xref="taxon:2126982"
                     /plasmid="p0.1229_3"
                     /geo_loc_name="USA:CA"
                     /collection_date="2000"
                     /collected_by="Dudley Lab/Penn State"
     gene            join(12600..12894,1..2711)
                     /locus_tag="C7X15_RS26250"
                     /old_locus_tag="C7X15_27360"
     CDS             join(12600..12894,1..2711)
                     /locus_tag="C7X15_RS26250"
                     /old_locus_tag="C7X15_27360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143760.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGTLGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            3094..3792
                     /locus_tag="C7X15_RS26255"
                     /old_locus_tag="C7X15_27365"
     CDS             3094..3792
                     /locus_tag="C7X15_RS26255"
                     /old_locus_tag="C7X15_27365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000939065.1"
                     /translation="MLQHKMNSSSYAKVHNVSSLEDIMSYHNDDVLLKFRKEWNVTPEE
                     ADDIFNETKKFIWLASTCLTECYNIKVHEQLQIIDEMWHTFIQFTDAYTSFCEKYLGAY
                     LHHYPNTNDMLKNEIRHVNEHGITFQEYRFNEYKNQIEKIAFYLGHETVAKWYGDYAVR
                     YSIKNINTIRIPKESISSDSYIEKVKSITHLPAAEFVKIIMRKDVWNDNGSVCGCSGKG
                     CGAGCSCNSR"
                     /name="hypothetical protein"
     gene            3831..5423
                     /locus_tag="C7X15_RS26260"
                     /old_locus_tag="C7X15_27370"
     CDS             3831..5423
                     /locus_tag="C7X15_RS26260"
                     /old_locus_tag="C7X15_27370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842142.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_001009226.1"
                     /translation="MNFIEKYIITFNKCNLFFIILLSTISSFLFVLFGYLIKTVIDNKD
                     SIGEINSLLIFIACFLSIRFLMPAGYSISEYLTQKTNIELSVKLREQVIDNIQNSHQEH
                     FLKKNKGELNKVIESMLSSASSLFYTICSDVVPLLIQMIGIIITICISVNTLIAIEFII
                     IMAIYIIFVIKMTQRRFPMMKSVALSSKFASGRMFDMMHMYPMDKAFHTTDKSRKRVIQ
                     AVNTHSEKQRKVNNEFFLFGISSAFLSVIFSSLIILSAYWMFLHGRASRGSIIMLATFL
                     FQVFLPLNRIGYLFRQIKMARTEIDLYCAEINDIKKTNYTDKQYLHVKDNICNIEIYNK
                     SFNHKLILTKGVVTFITGENGSGKTTIAKILSGNITTEENTIKINGIQQEKANSPFVNV
                     LYVPQDLDLMPGTIEENILHYSGINNLNTIKKLLHRFKFDKPLDYEIKGYGHNLSGGQK
                     QKLGVSLTSGKNVDLIIFDEPTKGFDSLGIKIISDYIKEENKEKHIIVISHDEQLINNI
                     PKAQIIDITKHEV"
                     /name="ABC transporter ATP-binding protein"
     gene            5426..6319
                     /locus_tag="C7X15_RS26265"
                     /old_locus_tag="C7X15_27375"
     CDS             5426..6319
                     /locus_tag="C7X15_RS26265"
                     /old_locus_tag="C7X15_27375"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_001061104.1"
                     /translation="MNNLIKKEIIEKFKKYNFQKYPFVFTDVNYKNLINWNDLNKLLEK
                     DILHYPRVRMANDNFPEIRGYKGFIRYTYSQTGDRTPHINRHQLYKCLRDGATLIVDRC
                     QSFFESVDESRLWLSKELECTCSANLYAAFTATPSFGLHFDNHDVIAVQIEGIKKWKVY
                     NPTYSYPLEDERSFDYLPPNTSPDYEFDITPGQAIYLPAGYWHNVTTQSKHSLHISFTV
                     IRPRRLELFKTLFDELKNNPYMREPIEHGDSLSDKEKIKTIITNAINSFDIQFAENMLK
                     AQSRTYRYKKIKLEHI"
                     /name="cupin domain-containing protein"
     gene            complement(6344..6706)
                     /locus_tag="C7X15_RS26270"
                     /old_locus_tag="C7X15_27380"
     CDS             complement(6344..6706)
                     /locus_tag="C7X15_RS26270"
                     /old_locus_tag="C7X15_27380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="WP_000181070.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVIY
                     KKHQLRKIDMRPARILPSIERYEYDVVMREFKGIACVSGKAEIKQNDGNILMAFIENWI
                     KVNGGWKMQFISYEKI"
                     /name="DUF4440 domain-containing protein"
     gene            complement(6703..6942)
                     /locus_tag="C7X15_RS26275"
                     /old_locus_tag="C7X15_27385"
     CDS             complement(6703..6942)
                     /locus_tag="C7X15_RS26275"
                     /old_locus_tag="C7X15_27385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="WP_000703021.1"
                     /translation="MKISDIEKNIYKGLAEFISQKIRDEHDICLGQFESEELLDEILKR
                     VIPVIYNVAIDDAIKSVRNVGDRLEEELDMRKVV"
                     /name="DUF2164 family protein"
     gene            complement(7653..7886)
                     /locus_tag="C7X15_RS26520"
     CDS             complement(7653..7886)
                     /locus_tag="C7X15_RS26520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137439.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(7893..8411)
                     /locus_tag="C7X15_RS26525"
     CDS             complement(7893..8411)
                     /locus_tag="C7X15_RS26525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673203.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(<8449..9099)
                     /locus_tag="C7X15_RS27065"
                     /pseudo=""
     CDS             complement(<8449..9099)
                     /locus_tag="C7X15_RS27065"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(9089..9412)
                     /locus_tag="C7X15_RS26295"
                     /old_locus_tag="C7X15_27405"
     CDS             complement(9089..9412)
                     /locus_tag="C7X15_RS26295"
                     /old_locus_tag="C7X15_27405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_112015440.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSSDRVQVVAALMAIERELCSLRQVVREQGAR
                     NDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            9477..9668
                     /locus_tag="C7X15_RS26300"
                     /old_locus_tag="C7X15_27410"
     CDS             9477..9668
                     /locus_tag="C7X15_RS26300"
                     /old_locus_tag="C7X15_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            10327..10485
                     /locus_tag="C7X15_RS27310"
                     /old_locus_tag="C7X15_27425"
     CDS             10327..10485
                     /locus_tag="C7X15_RS27310"
                     /old_locus_tag="C7X15_27425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071529495.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            complement(10836..11696)
                     /locus_tag="C7X15_RS26320"
                     /old_locus_tag="C7X15_27430"
     CDS             complement(10836..11696)
                     /locus_tag="C7X15_RS26320"
                     /old_locus_tag="C7X15_27430"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            complement(11879..12436)
                     /locus_tag="C7X15_RS26325"
                     /old_locus_tag="C7X15_27435"
     CDS             complement(11879..12436)
                     /locus_tag="C7X15_RS26325"
                     /old_locus_tag="C7X15_27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     ARG             complement(10840..11696)
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(9747..9826)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="94.0"
                     /evalue="1.83e-34"
     Typing          9857..10399
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.312"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(7550..9099)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.787"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(9096..9445)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.744"
                     /coverage="99.0"
                     /evalue="2.78e-61"
ORIGIN
        1 aaaatacccg ccgtgagcat gcagcgctga tacgtcagca ctatcagtat cgtgaatttg
       61 cctggccctg gacatttcgc cttacccgtc ttttatatac ccggagctgg ataagcaacg
      121 aacgtcctgg cctgcttttc gatctggcga cagggtggct tatgcaacat cgtattattc
      181 tccccggagc cactacgctg acccggttga tttcagaggt aagggaaaag gcgacgttgc
      241 gcctgtggaa caaactggca ctgataccgt cagccgaaca gcgttcacag ctggagatgc
      301 tgctggggcc aactgattgc agccgcctgt ctttactgga atcactgaaa aagggccctg
      361 tgaccatcag tggtccggcg tttaatgaag caattgaacg ctggaaaact ctgaacgatt
      421 ttggcctgca tgctgaaaac ctgagtacac tcccggctgt gcgcctgaaa aatctcgcac
      481 gttatgctgg tatgacttcg gtgttcaata ttgccaggat gtcaccgcag aaaaggatgg
      541 cggttctggt tgcctttgtc cttgcatggg aaacgctggc gctggatgat gcattggacg
      601 ttctggacgc catgctggcc gttatcatcc gtgacgccag aaagattggg cagaaaaaac
      661 ggctccgctc gctgaaggat ctggataaat ctgcattggc gctcgccagc gcatgttcgt
      721 acctgctgaa agaagaaaca ccggacgaat cgattcgtgc tgaggtgttc agctacatcc
      781 caaggcaaaa gctggctgaa atcatcacgc ttgtccgtga aattgcccgg ccctcagacg
      841 ataattttca tgaagaaatg gtggagcagt acgggcgcgt tcgtcgtttc ctgccccatc
      901 tgctgaatac cgttaaattt tcatccgcac ctgccggggt taccactctg aatgcctgtg
      961 actacctcag ccgggagttc agctcacggc ggcagttttt tgacgacgca ccaacggaaa
     1021 ttatcagtcg gtcatggaaa cggctggtga ttaacaagga aaaacatatc acccgcaggg
     1081 gatacacgct ctgctttctc agtaaactgc aggatagtct gaggcggagg gatgtctacg
     1141 ttaccggcag taaccggtgg ggagatcctc gtgcaagatt actacagggt gctgactggc
     1201 aggcaaaccg gattaaggtt tatcgttctt tggggcaccc gacagacccg caggaagcaa
     1261 taaaatctct gggtcatcag cttgatagtc gttacagaca ggttgctgca cgtctttgcg
     1321 aaaatgaggc tgtcgaactc gatgtttctg gcccgaagcc ccggttgaca atttctcccc
     1381 tcgccagtct tgatgagccg gacagtctga aacgactgag caaaatgatc agtgatctac
     1441 tccctccggt ggatttaacg gagttgctgc tcgaaattaa cgcccatacc ggatttgctg
     1501 atgagttttt ccatgctagt gaagccagtg ccagagttga tgatctgccc gtcagcatca
     1561 gcgccgtgct gatggctgaa gcctgcaata tcggtctgga accactgatc agatcaaatg
     1621 ttcctgcact gacccgacac cggctgaact ggacaaaagc gaactatctg cgggctgaaa
     1681 ctatcaccag cgctaatgcc agactggttg attttcaggc aacgctgcca ctggcacaga
     1741 tatggggtgg aggagaagtg gcatctgcag atggaatgcg ctttgttacg ccagtcagaa
     1801 caatcaatgc cggaccgaac cgcaaatact ttggtaataa cagagggatc acctggtaca
     1861 actttgtgtc cgatcagtat tccggctttc atggcatcgt tataccgggg acgctgaggg
     1921 actctatctt tgtgctggaa ggtcttctgg aacaggagac cgggctgaat ccaaccgaaa
     1981 ttatgaccga tacagcaggt gccagcgaac ttgtctttgg ccttttctgg ctgctgggat
     2041 accagttttc tccacgcctg gctgatgccg gtgcttcggt tttctggcga atggaccatg
     2101 atgccgacta tggcgtgctg aatgatattg ccagagggca atcagatccc cgaaaaatag
     2161 tccttcagtg ggacgaaatg atccggaccg ctggctccct gaagctgggc aaagtacagg
     2221 tttcagtgct ggtccgttca ttgctgaaaa gtgaacgtcc ttccggactg actcaggcaa
     2281 tcattgaagt ggggcgcatc aacaaaacgc tgtatctgct taattatatt gatgatgaag
     2341 attaccgccg gcgcattctg acccagctta atcggggaga aagtcgccat gccgttgcca
     2401 gagccatctg tcacggtcaa aaaggtgaga taagaaaacg atataccgac ggtcaggaag
     2461 atcaactggg cacactgggg ctggtcacta acgccgtcgt gttatggaac actatttata
     2521 tgcaggcagc cctggatcat ctccgggcgc agggtgaaac actgaatgat gaagatatcg
     2581 cacgcctctc cccgctttgc cacggacata tcaatatgct cggccattat tccttcacgc
     2641 tggcagaact ggtgaccaaa ggacatctga gaccattaaa agaggcgtca gaggcagaaa
     2701 acgttgctta acgtgagttt tcgttccact gagcgtcaga ccccggaacc tacaacacat
     2761 gtgtaaaacg tcaatggagg gggctattat cggactgcaa cacatttcgt acagccttta
     2821 cactcggtga attagcggcc ctagatgcat ccactcattc aaccaactca attctcttct
     2881 cttagagtgg aaaaattttg ttttccttgc agcccctacc cgtaaaaact ggctgcaaaa
     2941 accttgtatt actaatggat cctataacca ttaaaaaact tgattactat ctcatttata
     3001 gaattaggca tattgacaca agcacaaatt taacaatact ataataaatg ataactattc
     3061 tcatctacat tcaaatatat aattgggggt gttatgctac aacataagat gaacagcagt
     3121 tcttatgcaa aagttcataa tgttagctca ttagaagata tcatgagtta tcacaatgat
     3181 gatgttcttc taaaatttcg taaagaatgg aatgtaacac cagaagaagc tgatgatatc
     3241 tttaatgaaa caaagaaatt catctggcta gcctcaacat gcctaacgga atgctacaat
     3301 ataaaagttc acgagcaatt acaaattata gatgaaatgt ggcatacgtt tattcaattt
     3361 acagatgctt ataccagctt ttgtgaaaaa tatcttggtg cttacctcca ccattatcca
     3421 aatacaaatg acatgctaaa aaatgagata aggcatgtta atgagcatgg tataacattc
     3481 caagaatatc gttttaacga atataaaaat caaattgaaa aaatcgcttt ttacctgggt
     3541 catgaaactg tcgcaaagtg gtatggtgat tatgctgtaa gatacagcat aaagaacatt
     3601 aatactataa gaattccaaa agaatccata tccagtgatt cttacatcga aaaagtaaag
     3661 agtataactc accttccagc cgcagaattt gtaaaaataa taatgcgaaa agatgtttgg
     3721 aatgataatg gttctgtttg tggttgcagc ggtaaaggat gtggcgctgg gtgctcatgt
     3781 aattctagat aacataaagc ccgtaatata cgggctttaa ggattataaa ttgaatttta
     3841 ttgaaaagta catcatcaca tttaataaat gtaatttgtt ttttattata ttattatcaa
     3901 ctataagtag ttttcttttc gttctattcg gatatttaat aaaaacagtt attgacaata
     3961 aagattcaat aggagaaata aactcattat taatatttat agcatgtttc ttatcaatta
     4021 gattccttat gcctgctgga tatagcatat cggaatactt gactcaaaaa acaaatatag
     4081 aattatctgt aaagcttaga gagcaagtta ttgataatat acaaaattct caccaagagc
     4141 attttttaaa gaaaaataaa ggagaactaa ataaggttat agaaagcatg ctttcttctg
     4201 catcatcatt attttataca atatgttctg atgtagtacc attattaata caaatgattg
     4261 gaataatcat aacaatttgt ataagtgtta atacattaat agccattgag tttataataa
     4321 taatggcaat ctatataata tttgtcataa aaatgacaca acgtagattt ccaatgatga
     4381 aatcagttgc acttagctcc aaattcgcat ctggaagaat gtttgatatg atgcacatgt
     4441 atcctatgga taaagctttc cataccacgg ataaaagtcg taaacgagta attcaagctg
     4501 taaatacaca ttcagaaaaa caaagaaaag taaataatga atttttcctg tttggtatca
     4561 gctccgcttt tctttcagtc atttttagta gtctgattat cctatcagca tactggatgt
     4621 ttcttcatgg tagagcaagc cgtggtagta ttattatgct tgccactttt ttattccaag
     4681 tttttctccc attaaatcgc attggttatc tatttagaca aataaaaatg gcaagaacag
     4741 aaattgattt atactgtgcc gaaataaacg acataaaaaa aacaaattac acagacaagc
     4801 aatatcttca tgtaaaagac aatatttgca atattgaaat atacaacaaa tcattcaacc
     4861 ataaattaat attaacaaaa ggcgttgtta cctttataac cggtgagaat ggttcgggaa
     4921 aaacaactat tgcaaaaatt ctctctggca atataacaac agaagaaaac acaataaaaa
     4981 tcaatgggat acaacaagaa aaagcaaatt ctccttttgt taatgtctta tacgttcctc
     5041 aagatctaga tctcatgcct ggaactatag aggaaaatat attacattat tccggcatta
     5101 ataatttaaa tacaattaaa aagctgctac acagatttaa gttcgacaaa ccactagatt
     5161 atgagatcaa aggttacgga cataatttat ctggtggaca aaaacaaaaa ttaggagtat
     5221 ctcttacatc tggaaaaaac gtagacttga ttattttcga tgaaccgaca aaagggtttg
     5281 attcacttgg tataaaaata atcagtgatt atataaaaga ggaaaacaag gaaaagcata
     5341 tcattgttat atctcatgat gaacaactta taaataatat acctaaagca cagattatag
     5401 atatcacaaa acatgaggtt taaaaatgaa taatcttata aaaaaggaaa tcatagaaaa
     5461 atttaagaaa tataatttcc agaaatatcc gtttgtattc acggatgtta attataaaaa
     5521 cttaatcaac tggaatgatc ttaataaatt gcttgaaaaa gatatattgc actatcctag
     5581 agttagaatg gcaaatgaca actttcccga aattaggggg tataaaggat ttataagata
     5641 cacatatagc caaacaggcg atagaacacc acatataaat cgccatcagc tatataaatg
     5701 cttacgtgat ggcgcaaccc ttatagtaga tcgatgccag tcattctttg aatctgtaga
     5761 tgaaagcaga ctatggttat ctaaagagtt agaatgtaca tgtagtgcta atctatatgc
     5821 tgcatttaca gcaacaccaa gctttgggct tcattttgac aatcatgatg taatagctgt
     5881 tcaaattgag ggaataaaaa aatggaaagt ttacaaccct acctattcat accctctcga
     5941 agatgaaaga agcttcgatt atctaccacc taatacctcc ccagattatg agtttgatat
     6001 aaccccaggt caggccatat atcttcctgc tggatactgg cacaatgtta cgactcagag
     6061 caaacactct cttcatatat cttttacagt tataagacct cgtcgattag aactatttaa
     6121 aacattattt gatgaattaa aaaacaatcc atatatgcgg gaacctatag agcatggtga
     6181 ttcattatct gataaagaaa aaataaaaac tataataact aatgctataa atagttttga
     6241 tattcagttt gcagaaaaca tgcttaaagc tcaaagcaga acttatcgct ataaaaaaat
     6301 aaaacttgaa catatataac cattaatcta agggggcttc cccttagatt ttttcatacg
     6361 atataaactg cattttccat ccaccattaa cctttatcca attctcaata aatgccatta
     6421 atatatttcc atcattttgc tttatttcag ctttccctga aacacaagca atgcctttaa
     6481 actcacgcat tacgacatca tattcatatc tttctatgct aggtaaaatc ctcgctggac
     6541 gcatatcaat ctttcgtaac tgatgttttt tatagataac tttttgacca tttgttgaaa
     6601 taaaccaatc agcttccaag taatctagca tttcagtatt acctgaataa aatgcattat
     6661 accattcttt tcttattaga tttaagtctg atttaaaatt agtcacacta ccttcctcat
     6721 atctaattcc tcctctaatc tatccccaac attccttact gattttatag catcatcaat
     6781 agccacatta tatatgactg ggattactct tttaagaatc tcatcaagta attcctctga
     6841 ttcaaactgt ccaaggcata tatcatgttc atctctgatt ttttgtgaaa taaattcagc
     6901 tagtccttta tatatgtttt tttcaatatc agaaattttc attctttatg aagcctcaca
     6961 aataaattaa taatataaca tttttcctat cataggaaaa ttacaactca accatactgc
     7021 aacctggaat ttcccaagca agcatattta aataagtgca ggataccacc tcgaatcata
     7081 ctctaaggaa caagataatt taccataatc ccttattgta cgcaccgctg aaacgcgttc
     7141 ggcgcgatca cggcagcaga caggtaaaaa tggcaacaaa ccacccgaaa aactgccgcg
     7201 atcgcacccg gtaaatttta accacatgca tagctatgca gccatgtgaa tcacgcagga
     7261 attgccagcc agagacagct gaaacggatt tttttcatgc tttcggaagc gaagaagacg
     7321 gggaccggag cgggaaaaca gaagttcagg ggaatgagcg cgatctggca atagaggcaa
     7381 aacacagcaa caaaagacac accagaatcg cgcccgtatg cgttttaacg cgttcctgtg
     7441 ttttcagcaa ggtctgactg aagcgcgttc ccggtgcccc cgaaccaacc attatcccaa
     7501 aaaatgcacc ggaaacgcgc tgacaacatt ttagcctgtc tctgattacc atcccagcga
     7561 agggccatcc tgtgtccgtt cctgtatttc cagctggcgc tcacgctcca gggataactc
     7621 atgttcgcgt tcctgtatca gccgttcatc ccttatctcc tgttctcgct gtataactgg
     7681 ctcaagcgtt ctgtctgctc gctcaagtgc tgcacctgct gactcaactg catgacccgc
     7741 tcgttcagca tcgcgttgtc ccgttgcgta agcgaaaaca ttttctgcaa ttccacgaag
     7801 gcgctctccc attcgttcag ccgctgcata tagtcctgtt gcagttgctc taaggcgttc
     7861 agcaaatgtc tttccagctc cgtcactctg tgtcactccg tcagttgtac ccagtccttc
     7921 ccctgatagg gaatcaccgt tgccgatttc ccctgcggga taaccagaaa tttccttctc
     7981 ccgtcctgca caaactccac gccccatgtc ttcgcgttca gtttctgcaa tgtttcttcc
     8041 tgtatcctga tttcttccag gttcgcctgt atcatcccgc caagatacca gagcgtcccg
     8101 ccactcacgg taaacaggaa aaggaccatc cccagtaaca tcatgcccgt attccctgcc
     8161 agctttaaca cgtccttcct gtgctgcatc atcgcctctt tcaccccttc ccggtgtttt
     8221 ttcagtgatt cctctgtcga agctgtgaac agggctatag cgtctttgat tttcgtctcg
     8281 tttgatgtca cagccttgct tacagatttt tcgagcttgc tgaactcgtc gttcagcatt
     8341 gtctctgtag attcggctct ctctttcagc tttttctcga actccgcgcc cgtctgtaaa
     8401 agattgctca taaaatgctc ctttcagcct gatattcttc ccaccgttcg ggtctgcaat
     8461 gctgatactg cttcgcatca ccctgaccac ttcaagccct gcctctgtga gcgcctgaat
     8521 cacatcctga cggcctttta tctccccgac atgataaaga gcgtctatcc cgcgtgtgac
     8581 gctctctgca agcgcctgtt tcgtttccgg caggttatca gggagagtca gtatccggcg
     8641 gttctccggg gcgttggggt catgcagccc gtaatggtga ttaaccagcg tctgccaggc
     8701 atcaattctc ggcctgtctg cccggtcgta gtacggctgg aggcgttttc cggtctgtag
     8761 ctccatgttc gggatgacaa aattcagctc aagccgtccc ttgtcctggt gctccaccca
     8821 caggatgctg tactgattct tttcgagacc gggcatcagt acacgctcaa agctcgccat
     8881 caccttttca cgtcctcccg gcggcagctc cttctccgcg aacgacagaa cacctgacgt
     8941 gtatttcttc gcaaatggcg tggcatcgat gagttcccga acttcttccg gattaccctg
     9001 aagcaccgtt gccccttccc ggtttcgctc ccttcccagc aggtaatcaa ccggaccact
     9061 gccaccgcct tttcccctgg catgaaattt aacaatcatt ccgcgctccc tgttccctga
     9121 cgacctgccg taagctgcac aactctctct cgatggccat cagtgcggcc accacctgaa
     9181 cccggtcact ggaagaccac tgcccgctat tcaccttcct cgctgtctga ttcaggttat
     9241 tcccgatggc ggccagctga cgcagtaacg gcggtgccag tgtcggcagc ttcccggagc
     9301 gtgcaaccgg ctcacccagg cagacccgcc gcatccatac cgccagctgt ttaccctcac
     9361 agcgttccag taaccgggca tgttcatcat cagtaacccg tatggtgagc atcctctcgc
     9421 gtttcatcgg tatcattacc ccataaaaca gaaatccccc ttacacggag gcatcagtga
     9481 ctaaacagga aaaaaccgcc cttaacatgg cccgttttat cagaagccag acattaacgc
     9541 tgctggagaa actcaacgaa ctggacgcag atgaacaggc cgatatttgt gaatcacttc
     9601 acgaccacgc cgacgagctt taccgcagct gcctcgcacg cttcggggat gacggtgaaa
     9661 acctctgaca catacagctc ccggagacgg tcacagcttg tctgtgagcg gatgccggga
     9721 gcagacaagc ccgtcagggc gcgtcagggg gtttcggggc gaagccctga accagtcacg
     9781 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagtgcgaat tgtatggaaa
     9841 gcgcaccatg tccgatgtga aatgccgcac agatgcgtaa ggagaaaatg cacgttccgg
     9901 cgctcttccg cttcctcgct cactgactag ctacactcgg tcgttcggct gcggcgagcg
     9961 gtgtctgctc actcaaaagc ggaggtgcgg ttatccacag aatcagggga taacgccaaa
    10021 agaaacatgt gagcaaaaaa caagaaccag gaaaaggctg cgccgttggc gtttttccat
    10081 aggctccgcc cccctgacga gcatcataaa aatcgacgct caagtcagag gtggcgaaac
    10141 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    10201 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    10261 ctttctcata gctcacgctg taggtatctc ggctcggtgt aggtcgttcg ctccaagctg
    10321 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    10381 cttgagtcca acccggtaag acacgacaaa gcgccactgg cagcagccac tggtaactgg
    10441 acaaggtgga ttgagatatt aagagttctt gaagtggtgg cctaactgcg gctacactag
    10501 aaggacagta tttggtatct gtgctccacc aagccagtta cccggttaag cagtccccaa
    10561 ctgacttaac cttcgactaa accgcctccc caggcggttt tttcgtttac tggcagcaga
    10621 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat ctttttgaga tccgcccact
    10681 cctggaacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    10741 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    10801 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    10861 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    10921 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    10981 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    11041 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    11101 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc
    11161 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    11221 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    11281 gttgtcagaa gtaagttggc agcagtgtta tcactcatgg ttatggcagc actgcataat
    11341 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    11401 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
    11461 aataccgcac cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    11521 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    11581 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    11641 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgaa aatgttgaat actcatactc
    11701 ttcctttttc aatattattg aagcatttac cagggttatt gtctcatgag cggatacata
    11761 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    11821 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
    11881 acgaggccct ttcgtcttca agaattttat aaaccgtgga gcgggcaata ctgagctgat
    11941 gagcaatttc cgttgcacca gtgcccttct gatgaagcgt cagcacgacg ttcctgtcca
    12001 cggtacgcct gcggccaaat ttgattcctt tcagctttgc ttcctgtcgg ccctcattcg
    12061 tgcgttctag gatcctccgg cgttcagcct gtgccacagc cgacaggatg gtgaccacca
    12121 tttgccccat atcaccgtcg gtactgatcc cgtcatcaat gaaccggact gccacgccct
    12181 gagcgtcaaa ttcctttatc agttggatca tatcggcagt gtcgcggcca agacggtcga
    12241 gcttcttaac cagaatgaca tcaccttcct ccaccttcat cctcagcaaa tccagccctt
    12301 cccggtctgt tgaactgccg gatgccttat cggtaaatat acggtttgct ttcacacctg
    12361 cgtctttgag tgctctgacc tgaagatcaa gagactgctg actggttgag acccgagcgt
    12421 aaccaaaaag tcgcataaaa atgtacctta aatcgaatat cggacaactc atgtctatta
    12481 ttacaaattt acgatttaat agacatatta atgtaacagt tttacgatgt ccgataattt
    12541 ataacatttc gtacggttgg aaaaatgtta ctaaatgccc gtcaggcagg gaggccgata
    12601 tgcccgttga ctttctgacc actgagcaga ctgaaagcta tggcagattc accggtgaac
    12661 cggatgagct tcagctggca cgatattttc accttgatga agcagacaag gaatttatcg
    12721 gaaaaagcag aggtgatcac aaccgtctgg gcattgccct gcaaattgga tgtgtccgtt
    12781 ttctgggcac cttcctcacc gatatgaatc atattccttc cggcgtccgg cattttaccg
    12841 ccagacagct cgggattcgt gatatcaccg ttcttgcaga atacggtcag aggg
//
