LOCUS       NZ_CP028123             8120 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli O43 str. RM10042 plasmid pRM10042-3, complete
            sequence.
ACCESSION   NZ_CP028123
VERSION     NZ_CP028123.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08165042
            Assembly: GCF_003112205.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O43 str. RM10042
  ORGANISM  Escherichia coli O43 str. RM10042
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8120)
  AUTHORS   Parker,C.T., Huynh,S., Cooper,K., Bono,J.L. and Smith,T.P.
  TITLE     Genome Sequence of Escherichia coli O43 str. RM10042
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8120)
  AUTHORS   Parker,C.T., Huynh,S., Cooper,K., Bono,J.L. and Smith,T.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2018) Produce Safety and Microbiology Research
            Unit, United States Department of Agriculture, Agricultural Research
            Service, 800 Buchanan St, USDA ARS, Albany, CA 94710, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2018
            Assembly Method :: HGAP v.3 as available in the SMRT Analysis v.
            2.3; Geneious Reference Assembly v. 11.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 292.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003112205.1-RS_2024_06_16
            Annotation Date                   :: 06/16/2024 01:57:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,172
            CDSs (total)                      :: 5,051
            Genes (coding)                    :: 4,858
            CDSs (with protein)               :: 4,858
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 193
            CDSs (without protein)            :: 193
            Pseudo Genes (ambiguous residues) :: 0 of 193
            Pseudo Genes (frameshifted)       :: 96 of 193
            Pseudo Genes (incomplete)         :: 94 of 193
            Pseudo Genes (internal stop)      :: 53 of 193
            Pseudo Genes (multiple problems)  :: 45 of 193
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028123.1.
            Bacteria and source DNA available from Craig Parker, USDA ARS
            PSMRU, 800 Buchanan St. Albany, CA 94710.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8120
                     /organism="Escherichia coli O43 str. RM10042"
                     /mol_type="genomic DNA"
                     /strain="RM10042"
                     /serotype="O43"
                     /isolation_source="feces"
                     /host="Bos taurus"
                     /db_xref="taxon:1055541"
                     /plasmid="pRM10042-3"
                     /geo_loc_name="USA: California"
                     /lat_lon="36.68 N 121.67 W"
                     /collection_date="2009-10"
                     /collected_by="USDA ARS PSM"
     gene            1..2220
                     /gene="mobQ"
                     /locus_tag="I3W_RS27465"
                     /old_locus_tag="I3W_27460"
     CDS             1..2220
                     /gene="mobQ"
                     /locus_tag="I3W_RS27465"
                     /old_locus_tag="I3W_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_024224537.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAARET
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKEQDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSLGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGFCPV
                     LVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            2453..4198
                     /gene="col"
                     /locus_tag="I3W_RS27470"
                     /old_locus_tag="I3W_27465"
     CDS             2453..4198
                     /gene="col"
                     /locus_tag="I3W_RS27470"
                     /old_locus_tag="I3W_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="WP_009451403.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAVPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFDRFAHDPMSGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGKA
                     TGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRRKFWEEVSKDPELSKQ
                     FNPGNKKRLSQGLAPRARNKDTVGGRRSFELHHDKPISQDGGVYDMDNLRITTPKRHID
                     IHRGQ"
                     /name="col"
     gene            4201..4458
                     /locus_tag="I3W_RS27475"
                     /old_locus_tag="I3W_27470"
     CDS             4201..4458
                     /locus_tag="I3W_RS27475"
                     /old_locus_tag="I3W_27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000421100.1"
                     /translation="MELKNSISDYTETEFKKIIEDIINCEGDEKKQDDNLEHFISVTEH
                     PSGSDLIYYPEGNNDGSPEAVIKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            4520..4663
                     /locus_tag="I3W_RS27480"
                     /old_locus_tag="I3W_27475"
     CDS             4520..4663
                     /locus_tag="I3W_RS27480"
                     /old_locus_tag="I3W_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001314446.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            5128..5268
                     /locus_tag="I3W_RS27490"
                     /old_locus_tag="I3W_27485"
     CDS             5128..5268
                     /locus_tag="I3W_RS27490"
                     /old_locus_tag="I3W_27485"
                     /inference="COORDINATES: protein motif:HMM:NF020569.4"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]; GO:0043022 - ribosome binding
                     [Evidence IEA]; GO:0003723 - RNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /protein_id="WP_231237649.1"
                     /translation="MYEWDSQHGELEGYRASDGQHLGSFDPKTGNQLKGPDPKRNIKKY
                     L"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            5278..5535
                     /locus_tag="I3W_RS27495"
                     /old_locus_tag="I3W_27490"
     CDS             5278..5535
                     /locus_tag="I3W_RS27495"
                     /old_locus_tag="I3W_27490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_000523347.1"
                     /translation="MGLKLHINWFDKTTEEFKGGEYSKDFGDDGSVIERLGMPLKDNIN
                     NGWFDVIAEWVPLLQPYFNHQIDISGNEYFVSFDYRDGDW"
                     /name="colicin E3-like toxin immunity protein"
     gene            5555..5821
                     /locus_tag="I3W_RS27500"
                     /old_locus_tag="I3W_27495"
     CDS             5555..5821
                     /locus_tag="I3W_RS27500"
                     /old_locus_tag="I3W_27495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000861936.1"
                     /translation="MKVELKKSISDYTEAEFLELVKKICRAEGATEEDDNKLVREFERL
                     TEHPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            5883..6026
                     /locus_tag="I3W_RS27505"
                     /old_locus_tag="I3W_27500"
     CDS             5883..6026
                     /locus_tag="I3W_RS27505"
                     /old_locus_tag="I3W_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000724383.1"
                     /translation="MKKITGIILLLLAVIILSACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            6523..7449
                     /locus_tag="I3W_RS27515"
                     /old_locus_tag="I3W_27510"
     CDS             6523..7449
                     /locus_tag="I3W_RS27515"
                     /old_locus_tag="I3W_27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271358.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAIERALCEKLGADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNVSLPVPLSP
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKIGGAKSKRGAVATS
                     ARTLKPWETLGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(7667..7942)
                     /locus_tag="I3W_RS27520"
                     /old_locus_tag="I3W_27515"
     CDS             complement(7667..7942)
                     /locus_tag="I3W_RS27520"
                     /old_locus_tag="I3W_27515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091751.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPKPPAKPQNH"
                     /name="hypothetical protein"
     BGC             1..8120
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2..359)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.443"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(7947..8120)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          6524..7449
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.44"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..2217
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.917"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcgattt atcatctcag catgaaaatc atttcgcgaa aaaacggcta cagtgctgtt
       61 gcttctgctg cctaccgttc cggctctgtc atacccgatg accgtaccgg attaatccac
      121 gattacaccc gtaaacgcgg cgttgatgat gcggtcattc tcacccctgc gaatgcaccg
      181 tcctggtgtg gtgaccgttc cgttctctgg aatgcggtcg agaaagccga acagcgccgg
      241 aactcccagc tggcaaggga gattgaactc gccattcccc gtgagatttc ccgcgaggcc
      301 gcacgggaga ccgttctcgc ttttgtccgg gaaaactttg tcagtcgggg catgattgcc
      361 gatgtggcgt tccatcacat ggaccggacc aatccccatg cgcacatcat gctgaccacg
      421 agagctgtcg gggaaacggg attcgcagga aaggtcaggg actggaacga ccgggcactc
      481 gccgagacgt ggcgcgcatc atgggctgac catgcgaaca gagcgcttgc gaacgccggc
      541 taccaggaag agatagacca tcgttcatac gagcgtcagg gactggagaa agcgccgggc
      601 cttcacctcg gaaaggctgc ctgtgcgatg gaaaaacggg gaatggaaac agaacgcggt
      661 gagcagaacc gcctgattaa cagccttaac ctggaaatac aggtttcccg cacaaggctg
      721 gcagtcatcc aggtacagga gaacgtgcgg aaacgggaac tcagcgatgc cgcacgtcgt
      781 gcagcggaag cccttaacct gaccattccc gctgcgaatg cctcagcgga taccctgcgg
      841 gaattcattg ccacgttgcc gcaggaatgc ggcaacgcgt gggagatgac cccggagttc
      901 ctggcgatga gcgggaaggt gaacgacatc gaacgtgagg ggaatgcgct gctgaaagag
      961 caggccattc tcgaaaagga gatgaccgga ctcaaaaaag cacgccctgt cgcgtccctt
     1021 ctgtcagaga ttcccctgat gacatgggct gaaccggaat accgcaaaag acaactccgt
     1081 ttctggaaac tcgggaaaca gattgaatct cttcgccgca cctacagggc cgtgaaagaa
     1141 caggacattc ccgcccgtcg tcaggccttt gaaacgcagt ggaatacgtg gattgcgccg
     1201 ggaatggcag agctgaaaga aaaactgtca gcacgggaag cggagcggcg cagggaggaa
     1261 gccgaagcgg aagcgcgccg gaaggaacag gagcatgagg cgcggctgaa acgtcatgat
     1321 aaccaccgtc tgagccgtga aacggcatta gtcggggtta ttacggagct ggggcgtgcc
     1381 agagagccgg gaacgggcag gataacccgc tacatgatgc tgagtaacag agccggagaa
     1441 ttcacggtat ggggtgatga gctggcgcat tacccccaga gtgttcatga cccggtgaat
     1501 gtttacctgt cgctaggcgg ggctgtgatg gtctcggata tacgtgaggg aatgccagaa
     1561 tctcatgaga cgatggcgcg gcctgagcgt gtgagaatgt attccggtgc gacggtccgg
     1621 catgtactgg aacagatgcg ccaggggtgg ccctcttacg gttttccggc gctgccgcat
     1681 cactggccgg ataattttta tttcagcgac gaccgcaggc ccgtagcctc tccgctgccg
     1741 tcagcgcacc gggtggatat caccgctgat gctgtaccgg aacaactcat gcctgttgta
     1801 ttttcgagag agggaaacag caggacgctg aatctgttgt tatgcaaagg gccggaggaa
     1861 atgcttgtcg ggtttgttcg ccgggaagga gggttttgcc ccgttcttgt gcttccgtcg
     1921 ccggattaca gtcatctgat agtcagcacc atcacggaga acggggtatg cctggcaggt
     1981 tacggagaag ctataaacca tgatgaggat actccgtacc caccggaacc gcacctgatg
     2041 cagttccggc tcaaaggcca tcatgacagg cttttggctg ctgtccacaa accggaagag
     2101 atgccggatt atctcttccg tcaactcggt tttaatcaga cctggcatga gtggaagcgg
     2161 gacgaacagc acaggcaaca acaacgccgc cccgggcact tccggggcat gagtatgtga
     2221 tgtccggggc tgcaccccgg accccgccaa cacatcacgg gccacaaaat tttttgtggc
     2281 ccgctctgcg ttttctaagt gttatccctc ctgatttcta aaaaattttc cacctgagct
     2341 tgacagaaaa aacgatgacg ggtacttttt gatctgtaca taaaaccagt ggttttatgt
     2401 acagtattaa tcatgtaatt aattgtttta acgcttaaaa gagggaattt ttatgagcgg
     2461 tggcgatgga cgcggccata acacgggcgc gcatagcaca agtggtaaca ttaatggtgg
     2521 cccgaccggg cttggtgtag gtggtggtgc ttctgatggc tccggatgga gttcggaaaa
     2581 taacccgtgg ggtggtggtt ccggtagcgg cattcactgg ggtggtggtt ccggtcatgg
     2641 taatggcggg gggaatggta attccggtgg tggctcggga acaggcggta atctgtcagc
     2701 agtagctgtg ccagtggcat ttggttttcc ggcactttcc actccaggag ctggcggtct
     2761 ggcggtcagt atttcagcgg gagcattatc ggcagctatt gctgatatta tggctgccct
     2821 gaaaggaccg tttaaatttg gtctttgggg ggtggcttta tatggtgtat tgccatcaca
     2881 aatagcgaaa gatgacccca atatgatgtc aaagattgtg acgtcattac ccgcagatga
     2941 tattactgaa tcacctgtca gttcattacc tctcgataag gcaacagtaa acgtaaatgt
     3001 tcgtgttgtt gatgatgtaa aagacgaacg acagaatatt tcggttgttt caggtgttcc
     3061 gatgagtgtt ccggtggttg atgcaaaacc taccgaacgt ccgggtgttt ttacggcatc
     3121 aattccaggt gcacctgttc tgaatatttc agttaataac agtacgccag cagtacagac
     3181 attaagccca ggtgttacaa ataatactga taaggatgtt cgcccggcag gatttactca
     3241 gggtggtaat accagggatg cagttattcg attcccgaag gacagcggtc ataatgccgt
     3301 atatgtttca gtgagtgatg ttcttagccc tgaccaggta aaacaacgtc aggatgaaga
     3361 aaatcgccgt cagcaggaat gggatgctac gcatccggtt gaagcggctg agcgaaatta
     3421 tgaacgcgcg cgtgcagagc tgaatcaggc aaatgaagat gttgccagaa atcaggagcg
     3481 acaggctaaa gctgttcagg tttataattc gcgtaaaagc gaacttgatg cagcgaataa
     3541 gactcttgct gatgcaatag ctgaaataaa acaatttgat cgatttgccc atgacccaat
     3601 gtctggcggt cacagaatgt ggcaaatggc cgggcttaaa gcccagcggg cgcagacgga
     3661 tgtaaataat aagcaggctg catttgatgc tgctgcaaaa gagaagtcag atgctgatgc
     3721 tgcattaagt gccgcgcagg agcgccgcaa acagaaggaa aataaagaaa aggacgctaa
     3781 ggataaatta gataaggaga gtaaacggaa taagccaggg aaggcgacag gtaaaggtaa
     3841 accagttggt gataaatggc tggatgatgc aggtaaagat tcaggagcgc caattccaga
     3901 tcgcattgct gataagttgc gtgataaaga atttaaaaac ttcgacgatt ttcggagaaa
     3961 attctgggaa gaagtgtcga aagatcctga gttaagtaag caatttaatc caggtaataa
     4021 aaaacgcttg agccaaggat tagctccacg ggctaggaat aaagatactg tcggtgggcg
     4081 tcgctcattt gagcttcatc acgacaagcc aatcagtcag gatggtggtg tttatgatat
     4141 ggacaacctc cgtatcacta cgccgaaacg ccatattgat attcacagag gtcagtaaaa
     4201 atggaactaa aaaacagcat tagtgattac actgaaactg aattcaaaaa aattattgaa
     4261 gacatcatca attgtgaagg tgatgaaaaa aaacaggatg ataacctcga gcattttata
     4321 agtgttactg agcatcctag tggttctgat ctgatttatt acccagaagg taataatgat
     4381 ggtagccctg aagctgttat taaagagatt aaagaatggc gagctgctaa cggtaagtca
     4441 ggatttaaac agggctgaaa tatgaatgcc agttgtttaa ggatgaatga ctggcattct
     4501 ttcacaacaa ggagtcgtta tgaaaaaaat aacagggatt attttattgc ttcttgcagt
     4561 cattattctg gctgcatgtc aggcaaacta tatccgggat gttcagggcg ggaccgtatc
     4621 tccgtcatca acagctgaag tgaccggatt agcaacgcag taacccgaaa tcctctttga
     4681 caaaaacaaa gcgtgtcagg ctgattctga tgcgcttttt ttgaaatgtc acaaaagttc
     4741 catgtgggag atgggatcta aaatccctgt gcagaacctt ccatccggtg ggagaaaact
     4801 tgtcattttg acctgttcgc cattcggggg gggcgcttgg aaaacggaaa ttttcccacg
     4861 ctaagagaat tgagttgaac gagtggttac atctaggcca gctaactcac tgaacgtaaa
     4921 ggctgtacga aatgttccga ccgtccgata accatttggc ttacttccgt acatttcctt
     4981 gcgtagatgt acagaattgt acgatttcgg acaccaaaac cgctgttgtc ccgccattct
     5041 ggcgattcct gaaagttatt tgactaaaaa atagtcttaa cgtggtttat tttccgaatt
     5101 ccaagcggac cccataaagg ccgtaagatt tatgaatggg actcccagca cggtgaactt
     5161 gaagggtatc gtgccagtga tggtcagcat cttggctcat tcgatcctaa gactggtaat
     5221 cagttgaaag gacctgatcc aaaacgaaat atcaaaaagt atctttgaga ggatgttatg
     5281 gggcttaaat tacatattaa ttggtttgat aagacgaccg aggaatttaa aggtggtgag
     5341 tattcaaaag atttcggtga tgatggctcg gtcattgaac gtcttggaat gcctttaaaa
     5401 gataatatca ataatggttg gtttgatgtt atagctgaat gggtaccttt gctacaacca
     5461 tactttaatc atcaaattga tatttccggt aatgagtatt ttgtttcatt tgattatcgt
     5521 gatggtgatt ggtgatcaaa tattctcagg agagatgaag gtggagctaa agaaaagtat
     5581 tagtgattat accgaggctg aatttctgga gcttgtaaaa aaaatatgta gagctgaagg
     5641 tgctactgaa gaggatgaca ataaattagt gagagagttt gagcgattaa ctgagcaccc
     5701 agatggttca gatctgattt attatcctcg cgatgacagg gaagatagtc ctgaagggat
     5761 tgtcaaggaa attaaagaat ggcgagctgc taacggtaag tcaggattta aacagggctg
     5821 aaatatgaat gccggttgtt tatggatgaa tggctggcat tctttcacaa caaggagtcg
     5881 ttatgaaaaa aataacaggg attattttat tgcttcttgc agtcattatt ctgtctgcat
     5941 gtcaggcaaa ctatatccgg gatgttcagg gcgggaccgt atctccgtca tcaacagctg
     6001 aagtgaccgg attagcaacg cagtaacccg aaatcctctt tgacaaaaac aaagcgtgtc
     6061 aggctgattc tgatgcgctt ttttttttga aatgtcacaa aaattccatg tgggagatgg
     6121 gatctaaaat cctcgtgcag aactttccat ccagggggag aaaacttgtc gttttgagcc
     6181 gttcggtgtt cagaacgcac gaaaccgatc gcgcatcgct ttcgtgaata gttatgcagg
     6241 cccctgaaaa cgattctgac gcgttttttc ggttttgcct ggtgttttcc tgtctttttg
     6301 cgttttttgc gtcagaacgc gtctgagggc gttttaaggg gtgcgtacaa cgggagttat
     6361 ggtaaatgga tcgggttttc gggaaggttc gacaggattt gccgttgggt gtagtgtaag
     6421 cgactgaaaa acaaacgccc ctgaaattcg tgtctctccg ccaagattgt cacgagaata
     6481 caggggcgag gtcggtcgat aacctggaag gaatcgtaac acatgagcgc cgcgcttcaa
     6541 tacttcgaag aaaatttacc ccaccgtccc tatcacacgg atgatctcgc ttttggtctt
     6601 cgcatctccg gcaaagggcg tgcgcttctt gcgcggtaca tccagcagaa ccagcctcat
     6661 gcgcagttct ggctggtttt tgacgttgac cgcgaggggg ctgcgattga ctggagcgac
     6721 cggaacgccc ccgcgccgaa catcaccgta aaaaatcccg tgaacggtca tgctcacctg
     6781 ctctatgcac tcaacatcgc cgtgagaacc gcgcctgatg cgtcggttaa ggcactgaag
     6841 tacgctgccg cgatagagcg tgcgctgtgt gagaaacttg gtgcagatgt gaactacagc
     6901 ggactgattt gcaaaaatcc gttccacctg gaatggctgg tgatggagtg gcgcgaggaa
     6961 gcctataccc tcgatgaact ggctgattat ctcgatttga gcgcctcaga gcgtcgtagc
     7021 atcgataaac attacgggat ggggcgaaac tgccacctgt tcgaaatgac gcgcaaatgg
     7081 gcttacaggg cgattcgtca gggctggcct gtattctcac aatggcttga tgccgtgatc
     7141 cagcgtgtcg aaatgtacaa cgtatcgctt cccgttccgc tttctccggc ggaatgtcgg
     7201 gctattggca agagcattgc gaaatacacg cacaggaact tcacgccgga aactttcgca
     7261 cagtatgtgg ctgatacgca cacgccagaa attcaggctg cacgtggtcg caagggcggg
     7321 aaaattggag gtgctaagtc taagcgtggt gctgttgcca catctgcgcg aacgttgaaa
     7381 ccgtgggaga ctcttggaat tagccgcgcc tggtattacc aactgaaaaa acgaggtctt
     7441 gtagagtaga ccaaataaaa cctatatcag ataacagcgc ctttttggcg cttttttgag
     7501 cagcttggtt tgttgctatt tccctcgttg aatcccgcaa tggcgcggct ttccgcatga
     7561 ttgaggttgt agcgctcgcc gcagtctcat gaccgagcgt agcgagcgaa tgagcgagga
     7621 agcgcaaagg cgtccggtgg tgcatgtggc tcttacgcgc cggggcttag tggttctgcg
     7681 gtttcgcagg tggtttgggt agcttctcca gctcgttaat cagcggttgt agtcggttca
     7741 catccgcctg tcttgtgact tcctttcgca gaaactggag caggaacgca cgcagttgcg
     7801 cctcttccgg cctccgtacc ctcgccagca tggcagcccc cacaatgact ttttgcgccg
     7861 tgtccaggct ccggctcttc gccttcaggc gctgtaatct ggcctcagct tcggcaatct
     7921 tctgttcgag tgttctgctc attttgtgac tccgtgcgcg gtgaaaaatc gcattttatc
     7981 gcgtcactgg tagttgcaaa actgaactgg cataatgcac ggcacatcac gaagtgcgca
     8041 cttatacaat ctccacttcg tttcgattgt gtgcggtctg cgacgctaaa agaaaacggc
     8101 aaaaaggcat tacggcagaa
//
