LOCUS       NZ_CP028119             7072 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli O111 str. RM9322 plasmid pRM9322-2, complete
            sequence.
ACCESSION   NZ_CP028119
VERSION     NZ_CP028119.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08724616
            Assembly: GCF_003112245.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O111 str. RM9322
  ORGANISM  Escherichia coli O111 str. RM9322
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Parker,C.T., Huynh,S., Cooper,K., Bono,J.L. and Smith,T.P.
  TITLE     Genome Sequence of Escherichia coli O111 str. RM9322
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7072)
  AUTHORS   Parker,C.T., Huynh,S., Cooper,K., Bono,J.L. and Smith,T.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2018) Produce Safety and Microbiology Research
            Unit, United States Department of Agriculture, Agricultural Research
            Service, 800 Buchanan St, USDA ARS, Albany, CA 94710, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2018
            Assembly Method :: HGAP v.3 as available in the SMRT Analysis v.
            2.3; Geneious Reference Assembly v. 11.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 310.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003112245.1-RS_2024_06_16
            Annotation Date                   :: 06/16/2024 01:32:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,303
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,841
            CDSs (with protein)               :: 4,841
            Genes (RNA)                       :: 136
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 101
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 132 of 326
            Pseudo Genes (incomplete)         :: 185 of 326
            Pseudo Genes (internal stop)      :: 75 of 326
            Pseudo Genes (multiple problems)  :: 57 of 326
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028119.1.
            Bacteria and source DNA available from Craig Parker, USDA ARS
            PSMRU, 800 Buchanan St. Albany, CA 94710.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Escherichia coli O111 str. RM9322"
                     /mol_type="genomic DNA"
                     /strain="RM9322"
                     /serotype="O111"
                     /isolation_source="Creek"
                     /db_xref="taxon:1055542"
                     /plasmid="pRM9322-2"
                     /geo_loc_name="USA: Salinas, California"
                     /lat_lon="36.68 N 121.67 W"
                     /collection_date="2009-07"
                     /collected_by="USDA ARS PSM"
     gene            complement(826..972)
                     /locus_tag="I3O_RS28025"
                     /old_locus_tag="I3O_28015"
     CDS             complement(826..972)
                     /locus_tag="I3O_RS28025"
                     /old_locus_tag="I3O_28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000726755.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIGV
                     AVQ"
                     /name="colicin release lysis protein"
     gene            complement(1042..1305)
                     /locus_tag="I3O_RS28030"
                     /old_locus_tag="I3O_28020"
     CDS             complement(1042..1305)
                     /locus_tag="I3O_RS28030"
                     /old_locus_tag="I3O_28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="WP_001038407.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNIL
                     NCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
                     /name="colicin immunity domain-containing protein"
     gene            complement(1302..3395)
                     /gene="cda"
                     /locus_tag="I3O_RS28035"
                     /old_locus_tag="I3O_28025"
     CDS             complement(1302..3395)
                     /gene="cda"
                     /locus_tag="I3O_RS28035"
                     /old_locus_tag="I3O_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="WP_001286659.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPAAGN
                     NVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPEGSGVSPL
                     YISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGVNQPPVY
                     TQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFSNALEKVT
                     IPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGVVIEPGATS
                     VNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITLPAVVGAPSR
                     TILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDVGGLRDFIYWR
                     PDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNRETLTKFRDAIE
                     EHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKINPDADNGRIYLET
                     GEL"
                     /name="cda"
     gene            3659..3904
                     /locus_tag="I3O_RS28040"
                     /old_locus_tag="I3O_28030"
     CDS             3659..3904
                     /locus_tag="I3O_RS28040"
                     /old_locus_tag="I3O_28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000820629.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRARI
                     LKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
                     /name="hypothetical protein"
     gene            complement(4395..4628)
                     /locus_tag="I3O_RS28450"
     CDS             complement(4395..4628)
                     /locus_tag="I3O_RS28450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_112029644.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDNLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4635..5195)
                     /locus_tag="I3O_RS28455"
     CDS             complement(4635..5195)
                     /locus_tag="I3O_RS28455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<5230..5910)
                     /locus_tag="I3O_RS29400"
                     /pseudo=""
     CDS             complement(<5230..5910)
                     /locus_tag="I3O_RS29400"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5873..6196)
                     /locus_tag="I3O_RS28060"
                     /old_locus_tag="I3O_28050"
     CDS             complement(5873..6196)
                     /locus_tag="I3O_RS28060"
                     /old_locus_tag="I3O_28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_171844413.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGRWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6264..6455
                     /locus_tag="I3O_RS28065"
                     /old_locus_tag="I3O_28055"
     CDS             6264..6455
                     /locus_tag="I3O_RS28065"
                     /old_locus_tag="I3O_28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165972.1"
                     /translation="MTKQEKTALNMARFIKSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(<6613..6825)
                     /locus_tag="I3O_RS29405"
                     /pseudo=""
     CDS             complement(<6613..6825)
                     /locus_tag="I3O_RS29405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     BGC             1..7072
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(6541..6628)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.753"
                     /coverage="100.0"
                     /evalue="1.48e-35"
     Typing          complement(410..597)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.296"
                     /coverage="100.0"
                     /evalue="2.18e-82"
     Typing          complement(4289..5883)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.812"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6256..6458)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.235"
                     /coverage="100.0"
                     /evalue="2.48e-11"
ORIGIN
        1 aaaggaacaa atgctccgcc aggcgcttta ccgcttcctc gctcactgac tcgctgcgmt
       61 cggtcgttcg gctgcggcga gcggtatctg cccactcaaa agcggtgata gtgttatcca
      121 cagaatcagg ggataaggcc ggaaagaaca tgtgagcaaa aaggcaaagg gcaaaaaaag
      181 tcgcgccgct ggcgtttttc cataggctcc gcccccctga cggacttcac aaaacagacg
      241 ctttttctgt gcatctgtgg gagccatgag gctcaaccat gaaatccgcc agcggtggcg
      301 aaacccgacg tggcttaaag atcatcaccg tttccggtga atcgctccct cctgcgcccc
      361 cacgttcccg ccatatcgat taccggatac cttttccgcc tttctccctt gcgggacacg
      421 tggcgctttc tcatagctca cgtcttctgg tatctcgggg cgacttaggc agttcgctcc
      481 gggctgggct gtatgcagga accccccgtt cagcccgact gctgcacctt ctccggtaac
      541 tgttctcttg gggccaaccc ggaaaagaca cgacttaacg ccactggcag cagccattgg
      601 taactgattt agaggagttg ctcttgaagt tatgaaccag ttaaggctaa actgaaaaga
      661 cagttttggt gactgtgctc ctccaagcca gttaccaccg ccgattagaa gaggtcgaag
      721 acttctgatc ggacggaagg acccgccaca aggcgggttt tttcgtttac aggcagcaga
      781 ttacgcgcag aaaaaaagga tctcaagaag atcctttaat atttttcact gaaccgccac
      841 cccgattagt ttcgaagatg atgatggagc aattgttcca ccctgaacat cccttacata
      901 gttagcttgg caagcagtca gacacaaaac agcaagaacg agaatcatag atccccctat
      961 cttttttttc atgacatctc cttgtcatga gtgaatatct gacatttata cttaaatgcc
     1021 agatattaaa atcactcatg attataattt aaatttttcc aagatagatc ggacctcaac
     1081 cttaagtcca ttatcatcaa tttcataatc agccctatct gcatcaggct caaatcgctc
     1141 agcagccata aacaactctt ccccacaatt taatatattc ggagacaaat ccttaacacc
     1201 atacaatctt ctccgttcaa cacaaattcg ctctgaaaac tcaattgctg taattttcga
     1261 agctaaaaat aatttcgcca aatcgatcat tgccatcttg ttcatagttc acctgtctct
     1321 aaataaattc gaccattatc cgcatctgga tttattttcc acccagataa gaactcacca
     1381 tttgacttaa ttataaccac attcattgta ttaggattaa aataaacttt tgaacctttt
     1441 tctcttcggt atgttccttt ctctactgta tccttatccg ataaatgctc ctcaatagca
     1501 tccctgaatt tagtaagagt ttcacgattc ttttttgtat cactaatacc aaaatcacca
     1561 gcatgtttat attttttatc aagttgttta cgcgaaaatc gccctgaatc cagaggatca
     1621 ttaagcatca cataaacagc ttcaacacca gtcccagcag catcaggacg ccagtaaata
     1681 aaatctcgta gtccaccaac atcagaaaca ggagtaatcg tcgttactgg catttcgacc
     1741 ggttttactt ccgttcctgt atgcactggt gtaaccggaa cgggttgatg attacctgta
     1801 tctgttggca ccgaaggttg tggtaccgga ttaaccagaa tcgtccggga aggtgcgcct
     1861 actactgctg gtaacgtgat tttatccagt cctgttgctg tatcacggac agcattaaga
     1921 acaggaaccg cagctgggat actttcattc cctgttttca gtaaatccag agctaattgc
     1981 ccgttactgt ttatcaattg tccacgaaca ggcagattta cactcgtcgc accaggttca
     2041 atgaccacgc cctgtccagc caacatctga gcactcagcg aaaacatcgc ctccagattt
     2101 cgccccggca cgatatcact tcctgctcca gcttcttctg aatgaaacca cagagcacca
     2161 agagaaatcc gcataagcag gggactcact tcgggtatgg ttactttctc cagagcatta
     2221 ctgaaaaact tgcttatgtt gcctgcaata tcaccagcag cagtccagac accagttccg
     2281 gtcatcgcca gttgtacaga actggcagtg ttattcagaa caagagctcc ctgctcattc
     2341 attactgaat tagccctttc catcatttcc tgtgtatata caggaggctg gttcacacct
     2401 gctttactaa ataactcggc ttttgctttt tcagcttcag cccgggctac cgcttcttct
     2461 ttcttgactc tgaaatcatc ctttgcttta ttttcggctt cctgacgctg actcagacca
     2521 ttactgtcca gaatctctac agttgaaata tataacggag aaacaccact gccttcagga
     2581 aaacgaacaa tgacatcatg attattactg gacatcgtat aaccagcctc aacataatta
     2641 cctgtctcag caataactcc ttcttcctga atttcgggat tagacagaac cggagtattc
     2701 atctgaccca cacttaatac caggtccggt agatttggtg atacaggggc actgtaaaca
     2761 tctttaatat ttgttcccgg acgactccgg tccggaactg gaacggcatc aactaccggc
     2821 actgtcatcg gaatacctcc gataatagcc atatattgcg taccgttctg cataaggtcc
     2881 atcagacgat atttcaccgg tacattatta cccgctgctg gtaactggct ggccggaata
     2941 tccgttacct gttccagttg ctgtgtaacc ataagacgaa taattttttg ctcatttccg
     3001 gaagcaggct ccagacgggc aaaagaaaca gcagccgggc gagacggttc tggcaaacca
     3061 ccacgtaaac cattctcaag aactgttcgt gcagcggaaa ctttctcgtt tccccaaggg
     3121 ctatgcacac tgacagacca tcccgaccag tctccctctt caagttcctt atccagaata
     3181 atccggcgaa taattcccgg aaaaagaaca ctttgtacaa gtgcgatacc ctgtgggctg
     3241 taatcccccc atcccggagg catggatgga gcgatacctg atgagcctcc tggtttcaca
     3301 tcaccacccg aaatcagtcc tgttgacggc cacacgacca tcgagtgccc ataatcaata
     3361 ccttctgtcg gaccactacc ttcgtaatca ctcataaaaa cacctctttg acttttaaaa
     3421 caatgcgtta aaaacaacaa actgtacata aaaccactgg ttttatgtac agtaaaaagt
     3481 tacgtcacct cgtaaatcct gtcaacccac tggatttacg tttttgtgaa aacgctcaag
     3541 aaaaacaacc aatcattctt aaactcaatg gcaacaggca tgaaagaagg ctgattagca
     3601 taaggagaga gccaacagtt aaactgttat gacaccagag aaacacccgg agaccaccat
     3661 gaaacgccat agactgaccg aagccgaaga gcgccgactg gcagaagcca ttgccgctga
     3721 agctcgtgcc atggggctga cgaaaaagga caaaatgccg caggaaacaa agcgacgcct
     3781 ccgggcacgg atacttaaag ccattcttga tgaacgaacg gccagcaaaa atcgagaagc
     3841 cacactgact gccggacagg acccggatga caccttcacc tggcaaccac tgcgccgccg
     3901 ctaagctaag ggttatttaa cataacccct tattgtacgc accgctgaaa tgcgttcagc
     3961 gcgatcacgg ctgctgacag gtaaaaatgg caacaaacca cccgaaaagc tgccgcgatc
     4021 gcacctgata aattttaacc gtatgcatag ctattcagcc atgtgaataa cgctggtttt
     4081 gcctgcgtaa acctcatgac actgtttttt tttccatctt ttcagttgat gacatacgca
     4141 gacatcgcgg gatgaggctg aggaatgagc gcgatctggc aaagaggcaa aacacagcaa
     4201 caaaaacgac acgccagaat cgcgcccgga tgcgttttta acgcgttccg gtaccatctg
     4261 gcaacctccc ggaacaactc accgtcacat ccccattgac gggccacgcc atacccgtgc
     4321 ttcccgttcc tgctcttcat gccaggaccg cgcacgctcc cgttccaggc gtgcctgcct
     4381 ttcctgttca tcccttatct gctgttcgtg ataaataacc gactcaagcg gtccacctgc
     4441 ccggctaatc tctgcacctg ctgactcaag ttgtcgcact gttccctcag ttgcccgttc
     4501 tcctgtcgtg tcagctcgaa catatgctgc aaatccgtga aggcgctctc ccagtctttc
     4561 agccgctgcg tatagtcctg ctgcaattgc tctaaggcgt tcagtaagtg catttccagc
     4621 tctgtcatac tcacttactc cctgaccagt cttactgcgt tcttcttctc caccgtccag
     4681 ttgttttccc ccttcacccc ggacggcaac accagaaatt tcccgttcct gccctcgtga
     4741 tacgtcacac cccatgtttt ttcccggagt ttcgccagcg tctcttcctg gtccctgata
     4801 gccaggatgt tcgccgcaat ccggctttcc tgccactgaa tcagccccca tgacgccaga
     4861 aaaatcccgc ccgtgacgca aagcgccgtc agcgacgggt acagtatccg ccctttgacc
     4921 agcttccaga gcagctcttc ctgccgccgg gccagttcgt tctctgtggc gctgaactgc
     4981 gcgttcacgg cactgttcag cgtctccagt tgttctttca ccgctgctgt gtgtgcgctg
     5041 atagcgtctc tgattttctg cccgtttaag ttcagttccc tgtctacaga cgcttcgagc
     5101 ttcctgaact cgctgttcag catgttctct gtagagacgg cacgctcttt cagtttcttc
     5161 tcgaagtctg tccccatttg taaaagattg ctcatacagc gcccctttca gcctgagatt
     5221 acgcccgccc tccgggtcgg cgatactgat actgctcctg gttgtcctca ccacctcaaa
     5281 acctgccgct gtaagcgcct cagtgacatc ctgacgcgtt tttagcgctc cggcatggta
     5341 aagagcctcc agtcccctcg taatcgcttc tgcggcctcc tgtttcgctt tcggcagatt
     5401 attcggggtg acaagtgtcc gcctgttctc cggtgcgttc gggtcgtgca gcccgtaatg
     5461 gtgattcacc agtgtctgcc aggcattgat tcgcggacgg tccgcccggt cgtaataggg
     5521 ctggagccgt tttccgctcg ccagctccat attcgggatg acaaaattca gctcaagacg
     5581 ccccttgtcc tggtgctcca cccacaggat gctgtactga tttttttcaa gaccgggcat
     5641 cagtacccgc tcaaagctct ccatcaccct ttcacgctct cccggtggca gggtctgctc
     5701 tgcaaaagac agaacccccg aggtgtattt tttcgcaaac ggcgtggcat cgatgagttc
     5761 ccgcacctct tcgggagcac cccgcagaac tctcgcccct tcccggctac gctcccggcc
     5821 cagcaggtaa tcaaccggac cactgccacc gccttttccc ctggcatgaa acttaactat
     5881 catcccgttc tccctgttta cggacctcat ccctcagctc actcagttca cgtccgatgg
     5941 ccatcagtgc agccaccaca tgaacccggt catgccccga ccaccgtccg ctgtttatct
     6001 tccgggctat ctgattcagg ttattgccga ccgaagcgaa ctggcgcaac agcggcggtg
     6061 ccagtgtcgg aagacctgac gttttcgatg gcggtgcccc caggcagacc ttacgcatcc
     6121 atgacgcaag ttgttttccc tcacaacgtg ccagcagccg cgcatgttcc tcatccgtga
     6181 cccgtatcgt gagcatcctt tcgcgtttca ccggtatcat taaaaacctc cgacagactc
     6241 cccacacatg gagaaacaga actgtgacta aacaggaaaa aaccgccctt aacatggccc
     6301 gcttcatcaa aagccagacg ctgaccctgc tggaaaaact gaatgaactg gacgccgacg
     6361 accaggctga catctgcgaa gcgcttcacg accacgctga cgagctttac cgcagctgcc
     6421 tcgcacgctt cggggatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     6481 cagctggcct gtgagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtt
     6541 ttagcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     6601 gcttaatcgt ttagcatcag tgaggattgt atgaaaagtg caccatgccg ggtgtgaaat
     6661 gccgcacaga tgcgtaagga gaaaatgctc gtccaggcgc ttttccgctt cctcgctcac
     6721 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tctgcccact caaaagcggt
     6781 gatagtgtta tccacagaat caggggataa ggccggaaag aacatgtgag caaaaaggca
     6841 aagggcaaaa aaagtcgcgc cgctggcgtt tttccatagg ctccgccccc ctgacggact
     6901 tcacaaaaca gacgcttttt ctgtgcatct gtgggagcca tgaggctcaa ccatgaaatc
     6961 cgccagcggt ggcgaaaccc gacgtggctt aaagatcatc accgtttccg gtgaatcgct
     7021 ccctcctgcg cccccacgtt cccgccatat cgattaccgg ataccttttc cc
//
