LOCUS       NZ_CP028114             6673 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli O103 str. RM8385 plasmid pRM8385-2, complete
            sequence.
ACCESSION   NZ_CP028114
VERSION     NZ_CP028114.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08724650
            Assembly: GCF_003112165.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O103 str. RM8385
  ORGANISM  Escherichia coli O103 str. RM8385
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Parker,C.T., Huynh,S., Cooper,K., Bono,J.L. and Smith,T.P.
  TITLE     Genome Sequence of Escherichia coli O103 str. RM8385
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6673)
  AUTHORS   Parker,C.T., Huynh,S., Cooper,K., Bono,J.L. and Smith,T.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2018) Produce Safety and Microbiology Research
            Unit, United States Department of Agriculture, Agricultural Research
            Service, 800 Buchanan St, USDA ARS, Albany, CA 94710, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2018
            Assembly Method :: HGAP v.3 as available in the SMRT Analysis v.
            2.3; Geneious Reference Assembly v. 11.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 160.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003112165.1-RS_2024_06_16
            Annotation Date                   :: 06/16/2024 01:43:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,851
            CDSs (total)                      :: 5,720
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 99
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 355
            CDSs (without protein)            :: 355
            Pseudo Genes (ambiguous residues) :: 0 of 355
            Pseudo Genes (frameshifted)       :: 169 of 355
            Pseudo Genes (incomplete)         :: 183 of 355
            Pseudo Genes (internal stop)      :: 79 of 355
            Pseudo Genes (multiple problems)  :: 66 of 355
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028114.1.
            Bacteria and source DNA available from Craig Parker, USDA ARS
            PSMRU, 800 Buchanan St. Albany, CA 94710.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Escherichia coli O103 str. RM8385"
                     /mol_type="genomic DNA"
                     /strain="RM8385"
                     /serotype="O103"
                     /isolation_source="feces"
                     /host="Bos taurus"
                     /db_xref="taxon:1055540"
                     /plasmid="pRM8385-2"
                     /geo_loc_name="USA: Salinas, California"
                     /lat_lon="36.68 N 121.67 W"
                     /collection_date="2009-01"
                     /collected_by="USDA ARS PSM"
     gene            complement(join(6665..6673,1..255))
                     /locus_tag="I3Q_RS31070"
                     /old_locus_tag="I3Q_31050"
     CDS             complement(join(6665..6673,1..255))
                     /locus_tag="I3Q_RS31070"
                     /old_locus_tag="I3Q_31050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="WP_001038407.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNIL
                     NCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
                     /name="colicin immunity domain-containing protein"
     gene            complement(252..2345)
                     /gene="cda"
                     /locus_tag="I3Q_RS31075"
                     /old_locus_tag="I3Q_31055"
     CDS             complement(252..2345)
                     /gene="cda"
                     /locus_tag="I3Q_RS31075"
                     /old_locus_tag="I3Q_31055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="WP_001286660.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPAAGN
                     NVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPEGSGVSPL
                     YISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGVNQPPVY
                     TQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFSNALEKVT
                     IPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGVVIEPGATS
                     VNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITLPAVVGAPSR
                     TILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDVGGLRDFIYWR
                     PDAAGTGVEAVYVMLNEPLDSGRFSRKQLDKKYKHAGDFGISDTKKNRETLTKFRDAIE
                     EHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKINPDADNGRIYLET
                     GEL"
                     /name="cda"
     gene            2609..2854
                     /locus_tag="I3Q_RS31080"
                     /old_locus_tag="I3Q_31060"
     CDS             2609..2854
                     /locus_tag="I3Q_RS31080"
                     /old_locus_tag="I3Q_31060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000820629.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRARI
                     LKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
                     /name="hypothetical protein"
     gene            complement(3344..3577)
                     /locus_tag="I3Q_RS31580"
     CDS             complement(3344..3577)
                     /locus_tag="I3Q_RS31580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001345152.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDGLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3584..4144)
                     /locus_tag="I3Q_RS31585"
     CDS             complement(3584..4144)
                     /locus_tag="I3Q_RS31585"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001345151.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTVGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4179..4859)
                     /locus_tag="I3Q_RS32580"
                     /pseudo=""
     CDS             complement(<4179..4859)
                     /locus_tag="I3Q_RS32580"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4822..5145)
                     /locus_tag="I3Q_RS31100"
                     /old_locus_tag="I3Q_31080"
     CDS             complement(4822..5145)
                     /locus_tag="I3Q_RS31100"
                     /old_locus_tag="I3Q_31080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5213..5404
                     /locus_tag="I3Q_RS31105"
                     /old_locus_tag="I3Q_31085"
     CDS             5213..5404
                     /locus_tag="I3Q_RS31105"
                     /old_locus_tag="I3Q_31085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165972.1"
                     /translation="MTKQEKTALNMARFIKSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6449..6595)
                     /locus_tag="I3Q_RS31125"
                     /old_locus_tag="I3Q_31105"
     CDS             complement(6449..6595)
                     /locus_tag="I3Q_RS31125"
                     /old_locus_tag="I3Q_31105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000726755.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIGV
                     AVQ"
                     /name="colicin release lysis protein"
     BGC             1..6673
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5490..5574)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          complement(6033..6220)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.296"
                     /coverage="100.0"
                     /evalue="2.18e-82"
     Typing          complement(3238..4832)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.872"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5205..5407)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.235"
                     /coverage="100.0"
                     /evalue="2.47e-11"
ORIGIN
        1 aaatttttcc aagatagatc ggacctcaac cttaagtcca ttatcatcaa tttcataatc
       61 agccctatct gcatcaggct caaatcgctc agcagccata aacaactctt ccccacaatt
      121 taatatattc ggagacaaat ccttaacacc atacaatctt ctccgttcaa cacaaattcg
      181 ctctgaaaac tcaattgctg taattttcga agctaaaaat aatttcgcca aatcgatcat
      241 tgccatcttg ttcatagttc acctgtctct aaataaattc gaccattatc cgcatctgga
      301 tttattttcc acccagataa gaactcacca tttgacttaa ttataaccac attcatcgta
      361 ttaggattaa aataaacttt tgaacctttt tctcttcggt atgttccttt ctctactgta
      421 tccttatccg ataaatgctc ctcaatagca tccctgaatt tagtaagagt ttcacgattc
      481 ttttttgtat cactaatacc aaaatcacca gcatgtttat attttttatc aagttgttta
      541 cgcgaaaatc gccctgaatc cagaggttca ttaagcatca cataaacagc ttcaacacca
      601 gtcccagcag catcaggacg ccagtaaata aaatctcgta gtccaccaac atcagaaaca
      661 ggagtaatcg tcgttactgg catttcgacc ggttttactt ccgttcctgt atgcactggt
      721 gtaaccggaa cgggttgatg attacctgta tctgttggca ccgaaggttg tggtaccgga
      781 ttaaccagaa tcgtccggga aggtgcgcct actactgctg gtaacgtgat tttatccagt
      841 cctgttgctg tatcacggac agcattaaga acaggaaccg cagctgggat actttcattc
      901 cctgttttca gtaaatccag agctaattgc ccgttactgt ttatcaattg tccacgaaca
      961 ggcagattta cactcgtcgc accaggttca atgaccacgc cctgtccagc caacatctga
     1021 gcactcagcg aaaacatcgc ctccagattt cgccccggca cgatatcact tcctgctcca
     1081 gcttcttctg aatgaaacca cagagcacca agagaaatcc gcataagcag gggactcact
     1141 tcgggtatgg ttactttctc cagagcatta ctgaaaaact tgcttatgtt gcctgcaata
     1201 tcaccagcag cagtccagac accagttccg gtcatcgcca gttgtacaga actggcagtg
     1261 ttattcagaa caagagctcc ctgctcattc attactgaat tagccctttc catcatttcc
     1321 tgtgtatata caggaggctg gttcacacct gctttactaa ataactcggc ttttgctttt
     1381 tcagcttcag cccgggctac cgcttcttct ttcttgactc tgaaatcatc ctttgcttta
     1441 ttttcggctt cctgacgctg actcagacca ttactgtcca gaatctctac agttgaaata
     1501 tataacggag aaacaccact gccttcagga aaacgaacaa tgacatcatg attattactg
     1561 gacatcgtat aaccagcctc aacataatta cctgtctcag caataactcc ttcttcctga
     1621 atttcgggat tagacagaac cggagtattc atctgaccca cacttaatac caggtccggt
     1681 agatttggtg atacaggggc actgtaaaca tctttaatat ttgttcccgg acgactccgg
     1741 tccggaactg gaacggcatc aactaccggc actgtcatcg gaatacctcc gataatagcc
     1801 atatattgcg taccgttctg cataaggtcc atcagacgat atttcaccgg tacattatta
     1861 cccgctgctg gtaactggct ggccggaata tccgttacct gttccagttg ctgtgtaacc
     1921 ataagacgaa taattttttg ctcatttccg gaagcaggct ccagacgggc aaaagaaaca
     1981 gcagccgggc gagacggttc tggcaaacca ccacgtaaac cattctcaag aactgttcgt
     2041 gcagcggaaa ctttctcgtt tccccaaggg ctatgcacac tgacagacca tcccgaccag
     2101 tctccctctt caagttcctt atccagaata atccggcgaa taattcccgg aaaaagaaca
     2161 ctttgtacaa gtgcgatacc ctgtgggctg taatcccccc atcccggagg catggatgga
     2221 gcgatacctg atgagcctcc tggtttcaca tcaccacccg aaatcagtcc tgttgacggc
     2281 cacacgacca tcgagtgccc ataatcaata ccttctgtcg gaccactacc ttcgtaatca
     2341 ctcataaaaa cacctctttg acttttaaaa caatgcgtta aaaacaacaa actgtacata
     2401 aaaccactgg ttttatgtac agtaaaaagt tacgtcacct cgtaaatcct gtcaacccac
     2461 tggatttacg tttttgtgaa aacgctcaag aaaaacaacc aatcattctt aaactcaatg
     2521 gcaacaggca tgaaagaagg ctgattagca taaggagaga gccaacagtt aaactgttat
     2581 gacaccagag aaacacccgg agaccaccat gaaacgccat agactgaccg aagccgaaga
     2641 gcgccgactg gcagaagcca ttgccgctga agctcgtgcc atggggctga cgaaaaagga
     2701 caaaatgccg caggaaacaa agcgacgcct ccgggcacgg atacttaaag ccattcttga
     2761 tgaacgaacg gccagcaaaa atcgagaagc cacactgact gccggacagg acccggatga
     2821 caccttcacc tggcaaccac tgcgccgccg ctaagctaag ggttatttaa cataacccct
     2881 tattgtacgc accgctgaaa tgcgttcagc gcgatcacgg ctgctgacag gtaaaaatgg
     2941 caacaaacca cccgaaaagc tgccgcgatc gcacctgata aattttaacc gtatgcatag
     3001 ctattcagcc atgtgaataa cgctggtttt gcctgcgtaa acctcatgac actgtttttt
     3061 ttccatcttt tcagttgatg acatacgcag acatcgcggg atgaggctga ggaatgagcg
     3121 cgatctggca aagaggcaaa acacagcaac aaaaacgaca cgccagaatc gcgcccggat
     3181 tcgtttttaa cgcgttccgg taccatctgg caacctcccg gaacaactca ccgtcacatc
     3241 cccattgacg ggccacgcca tacccgtact tcccgttcct gctcttcatg ccaggaccgc
     3301 gcacgctccc gttccaggcg tgccagcctt tcctgttcat cccttatctg ctgttcgtga
     3361 taaataaccg actcaagcgg tccacctgcc cggctaatct ctgcacctgc tgactcaagc
     3421 cgtcgcactg ttccctcagt tgcccgttct cctgtcgtgt cagctcgaac atatgctgca
     3481 aatccgtgaa ggcgctctcc cagtctttca gccgctgcgt atagtcctgc tgcaattgct
     3541 ctaaggcgtt cagtaagtgc atttccagct ctgtcatact cacttactcc ctgaccagtc
     3601 ttactgcgtt cttcttctcc accgtccagt tgttttcccc cttcaccccg gacggcaaca
     3661 ccagaaattt cccgttcctg ccctcgtgat acgtcacacc ccatgttttt tcccggagtt
     3721 tcgccagcgt ctcttcctgg tccctgatag ccaggatgtt cgccgcaata cggctttcct
     3781 gccactgaat caacccccat gacgccagaa aaatcccgac cgtgacgcaa agcgccgtca
     3841 gcgacgggta cagtatccgc cctttgacca gcttccagag cagctcttcc tgccgccggg
     3901 caagttcgtt ctctgtggcg ctgaactgcg cgttcacggc actgttcagc gtctccagtt
     3961 gttctttcac cgctgctgtg tgtgcgctga tagcgtctct gattttctgc ccgtttaagt
     4021 tcagttccct gtctacagac gcttcgagct tcctgaactc gctgttcagc atgttctctg
     4081 tagagacggc acgctctttc agtttcttct cgaagtctgt ccccatttgt aaaagattgc
     4141 tcatacagcg cccctttcag cctgagatta cgcccgccct ccgggtcggc gatactgata
     4201 ctgctcctgg ttgtcctcac cacctcaaaa cctgccgctg taagcgcctc agtgacatcc
     4261 tgacgcgttt ttagcgctcc ggcatggtaa agagcctcca ctcccctcgt aatcgcttct
     4321 gcggcctcct gtttcgcttt cggcagatta ttcggggtga caagtgtccg cctgttctcc
     4381 ggtgcgttcg ggtcgtgcag cccgtaatgg tgattcacca gtgtctgcca ggcattgatt
     4441 cgcggacggt ccgcccggtc gtaatagggc tggagccgtt ttccgctcgc cagctccata
     4501 ttcgggatga caaaattcag ctcaagacgc cccttgtcct ggtgctccac ccacaggatg
     4561 ctgtactgat ttttttcaag accgggcatc agtacccgct caaagctctc catcaccctt
     4621 tcacgctctc ccggtggcag tgtctgctct gcaaaagaca gaacccccga ggtgtatttt
     4681 ttcgcaaacg gcgtggcatc gatgagttcc cgcacctctt cgggagcacc ccgcagaact
     4741 ctcgcccctt cccggctacg ctcccggccc agcaggtaat caaccggacc actgccaccg
     4801 ccttttcccc tggcatgaaa cttaactatc atcccgttct ccctgtttac ggacctcatc
     4861 cctcagctca ctcagttcac gtccgatggc catcagtgca gccaccacat gaacccggtc
     4921 atgccccgac cactgtccgc tgtttatctt ccgggctatc tgattcaggt tattgccgac
     4981 cgaagcgaac tggcgcaaca gcggcggtgc cagtgtcgga agacctgacg ttttcgatgg
     5041 cggtgccccc aggcagacct tacgcatcca tgacgcaagt tgttttccct cacaacgtgc
     5101 cagcagccgc gcatgttcct catccgtgac ccgtatcgtg agcatccttt cgcgtttcac
     5161 cggtatcatt aaaaacctcc gacagactcc ccacacatgg agaaacagaa ctgtgactaa
     5221 acaggaaaaa accgccctta acatggcccg cttcatcaaa agccagacgc tgaccctgct
     5281 ggaaaaactg aatgaactgg acgccgacga ccaggctgac atctgcgaag cgcttcacga
     5341 ccacgctgac gagctttacc gcagctgcct cgcacgcttc ggggatgacg gtgaaaacct
     5401 ctgacacatg cagctcccgg agacggtcac agctggcctg tgagcggatg ccgggagcag
     5461 acaagcccgt cagggcgcgt cagcgggttt tagcgggtgt cggggcgcag ccatgaccca
     5521 gtcacatagc gatagcggag tgtatactgg cttaatcata tggcatcagt gagaattgta
     5581 tgaaaagtgc gccatggcgg gtgtgaaatg ccgcacagat gcgtaaggag aaaatgctcg
     5641 tccaggcgct tttccgcttc ctcgctcact gactcgctac gctcggtcgt tcggctgcgg
     5701 cgagcggtat ctgcccactc aaaagcggtg atagtgttat ccacagaatc aggggataag
     5761 gccggaaaga acatgtgagc aaaaaggcaa agggcaaaaa aagtcgcgcc gctggcgttt
     5821 ttccataggc tccgcccccc tgacggactt cacaaaacag acgctttttc tgtgcatctg
     5881 tgggagccat gaggctcaac catgaaatcc gccagcggtg gcgaaacccg acgtggctta
     5941 aagatcatca ccgtttccgg tgaatcgctc cctcctgcgc ccccacgttc ccgccatatc
     6001 gattaccgga taccttttcc gcctttctcc cttgcgggac acgtggcgct ttctcatagc
     6061 tcacgtcttc tggtatctcg gggcgactta ggcagttcgc tccgggctgg gctgtatgca
     6121 ggaacccccc gttcagcccg actgctgcac cttctccggt aactgttctc ttggggccaa
     6181 cccggaaaag acacgactta acgccactgg cagcagccat tggtaactga tttagaggag
     6241 ttgctcttga agttatgaac cagttaaggc taaactgaaa agacagtttt ggtgactgtg
     6301 ctcctccaag ccagttacca ccgccgatta gaagaggtcg aagacttctg atcggacgga
     6361 aggacccgcc acaaggcggg ttttttcgtt tacaggcagc agattacgcg cagaaaaaaa
     6421 ggatctcaag aagatccttt aatatttttc actgaaccgc caccccgatt agtttcgaag
     6481 atgatgatgg agcaattgtt ccaccctgaa catctcttac atagttagct tggcaagcag
     6541 tcagacacaa aacagcaaga acgagaatca tagatccccc tatctttttt ttcatgacat
     6601 ctccttgtca tgagtgaata tctgacattt atacttaaat gccagatatt aaaatcactc
     6661 atgattataa ttt
//
