LOCUS       NZ_CP027529             8731 bp    DNA     circular CON 04-JAN-2024
DEFINITION  Acinetobacter baumannii strain AR_0083 plasmid unnamed, complete
            sequence.
ACCESSION   NZ_CP027529
VERSION     NZ_CP027529.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04014924
            Assembly: GCF_002996805.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Conlan,S., Thomas,P.J., Mullikin,J., Frank,K.M. and Segre,J.A.
  TITLE     Whole genome sequencing of Acinetobacter baumannii AR_0083
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8731)
  AUTHORS   Segre,J.A. and Mullikin,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2018) NIH Intramural Sequencing Center, 5625
            Fishers Lane, Rockville, MD 20852, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3
            Genome Coverage                   :: 220x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 16:33:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,042
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,838
            CDSs (with protein)               :: 3,838
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 35 of 108
            Pseudo Genes (incomplete)         :: 65 of 108
            Pseudo Genes (internal stop)      :: 22 of 108
            Pseudo Genes (multiple problems)  :: 12 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP027529.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="AR_0083"
                     /db_xref="taxon:470"
                     /plasmid="unnamed"
     gene            44..2455
                     /gene="znuD2"
                     /locus_tag="AM462_RS20315"
                     /old_locus_tag="AM462_20315"
     CDS             44..2455
                     /gene="znuD2"
                     /locus_tag="AM462_RS20315"
                     /old_locus_tag="AM462_20315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            2760..3221
                     /locus_tag="AM462_RS20320"
                     /old_locus_tag="AM462_20320"
     CDS             2760..3221
                     /locus_tag="AM462_RS20320"
                     /old_locus_tag="AM462_20320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(3544..3717)
                     /locus_tag="AM462_RS20555"
     CDS             complement(3544..3717)
                     /locus_tag="AM462_RS20555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282484.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQN"
                     /name="hypothetical protein"
     gene            complement(3717..4088)
                     /locus_tag="AM462_RS20325"
                     /old_locus_tag="AM462_20325"
     CDS             complement(3717..4088)
                     /locus_tag="AM462_RS20325"
                     /old_locus_tag="AM462_20325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            complement(4360..4614)
                     /locus_tag="AM462_RS20865"
                     /old_locus_tag="AM462_20330"
     CDS             complement(4360..4614)
                     /locus_tag="AM462_RS20865"
                     /old_locus_tag="AM462_20330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014303.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     gene            5298..6248
                     /gene="repM"
                     /locus_tag="AM462_RS20340"
                     /old_locus_tag="AM462_20340"
     CDS             5298..6248
                     /gene="repM"
                     /locus_tag="AM462_RS20340"
                     /old_locus_tag="AM462_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            6241..6816
                     /locus_tag="AM462_RS20345"
                     /old_locus_tag="AM462_20345"
     CDS             6241..6816
                     /locus_tag="AM462_RS20345"
                     /old_locus_tag="AM462_20345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            6836..6979
                     /locus_tag="AM462_RS20560"
     CDS             6836..6979
                     /locus_tag="AM462_RS20560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(7168..7686)
                     /locus_tag="AM462_RS20350"
                     /old_locus_tag="AM462_20350"
     CDS             complement(7168..7686)
                     /locus_tag="AM462_RS20350"
                     /old_locus_tag="AM462_20350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            8059..8346
                     /locus_tag="AM462_RS20355"
                     /old_locus_tag="AM462_20355"
     CDS             8059..8346
                     /locus_tag="AM462_RS20355"
                     /old_locus_tag="AM462_20355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            8333..8647
                     /locus_tag="AM462_RS20360"
                     /old_locus_tag="AM462_20360"
     CDS             8333..8647
                     /locus_tag="AM462_RS20360"
                     /old_locus_tag="AM462_20360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     Typing          5299..6248
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttaagtgtt atattataat ataacattaa atttggttgt tttatgctaa ataaaagtaa
       61 actattttta gctttaatta ctttaggggc ctcgaaaatt ttattggcag ctgaagggcc
      121 tgttactacc ttaaatacaa tagtattaac tgcacaatct gatgagttag ggtctgagct
      181 gttaggcaaa agtctaaatg ttagtaatca gtttattgat actagtaaac ttaaacagcg
      241 ttcgacaact ctaggggatg ccttgggtac ggaattagga attcattcta atcaatatgg
      301 aggtggagca tctactccta ttattcgtgg gcaggaagga aaacgtatta aagttttaca
      361 gaataatgct gacgtgttgg atatgtctaa tatgtcccca gatcacgctg tcacagttga
      421 accaagttta gctaagtcta ttgaaattat acgtggtgca agtacccttt tgtatagttc
      481 aaatagtgcg gcaggggtag tcaatgtcat tgactataaa attccaaccc aaatgcctca
      541 agacggatta gagggtaata ctacattacg ttttaatact ggcagtaatg agaagttgac
      601 cacagcaggt gtaacagttg gcttaagccc acatgtagct ttacgggcag aagggttata
      661 tcgtaatgct ggtaactata aaactcctca ttaccagtct agctcttata attctttaga
      721 agatcttgaa aaccaaaaca gtatttataa aaatttaaaa tatttaccag aaagctgggc
      781 agagtcgaga gtaggtacgc ttggattatc ctggatagac gataatactt atttaggagt
      841 ttcttataca catcgtcatg atgaatatgg tttaccagcc catagccatt tatatgaagg
      901 ttgtggtgcc tctgcaatcg gtatcgattc acgaattagt ggattaaaaa attatctttt
      961 atattatcct caattgatgg atgagcaaga tattaattac ataaatccac gtcctgattg
     1021 tcatcagcat aatcatattc atgaaactaa tttctctcat aatgctcctt atattgattt
     1081 aaatactcga cgttatgatg tgcgtggaga attcactcaa ccatttaccg gtattgacaa
     1141 aattcgtacc tcattgagtt atatagatta ttttcacaat gaactcgagg gagataagat
     1201 tactaacttt tttaaaaata caggaaaagt aggccgcata gagttatcac atcagccatt
     1261 aggggaatta accggtatct tggggctaca atatttggaa caggataata gtgctttatc
     1321 accagtccat tctcaagaag gccataccac ctatctagat aatcagcagc tattaaatcg
     1381 aaatgtcact aaaaacttta gtgtatttgg cctagaaaaa tataactgga acgactttac
     1441 tttcgaacta ggtgctcgta tagaaaagca gaaagttagt atggattatg atatagaaaa
     1501 aattaaagat tcaatgaaac cttggcctaa taaatataac agtccttatg tagaaaaaaa
     1561 taataaaatc agggctcaaa atttaaaatc tattttagaa gctgttcaac ctaacaaaga
     1621 aacagctttt tcttatgcag gtacagtcca ctggagattt gctccaaatt atatcttatc
     1681 attaacaggt acacatcaag aaagattacc taatgcacaa gaaatgtata ctcatggtat
     1741 gcatctcgct actaattctt ttgaaatagg taaccgcttt ctaagaaaag agaaatccaa
     1801 taatttagaa atcagtctgg cctataagga tgatttactt gattatcaaa tcagtactta
     1861 ctattatgat tttgataact atatatattt acagacactc aatgaagttt taggaactac
     1921 aaaagttaga gatcagcata cactacgtat taatcattat agccagtctg ctgctaattt
     1981 ttatggatta gagggcaaca tagggtatca atttaattct gtttatcacg gctctctttt
     2041 tggagattat gtgaaaggcc gtttaacaaa tttacctgat gctgtaattg catacgatat
     2101 ctggaataga gaaccgactt tagctcccca aaaggatcgt tacactccta gattacctcc
     2161 tgctcgtttg ggtacacgac taaaagctga ttttgatgaa tcgcttaaag gtgaaataga
     2221 atattatcga gtctttaaac aagataatat ttctaaattt gagcaggtta catcaggtta
     2281 taatatgctt aatatgactt tagcatataa aaataagcta tctcacacag aatatgattt
     2341 attctttaaa gcgaataatt tacttgatca gaaagtctat gcccatgaaa catttttgcc
     2401 atatattccc caaattggaa gaaattttag tttaggctta aatcttaatt tctagataag
     2461 atactgtaat cttctaagaa aatttcttag aagatttttc ttataaaatg attagattaa
     2521 taattaaggt aagcgtccgc tgaatcccat acctattttt taattcgatt taggtttcca
     2581 gcagtgtggc agtaactctt caatctgggt cactttatgt gtcggcagcc ttttcagcac
     2641 tgaatagcca ccgattttgt agacaccttt tagctagata aaaagcaatt tttttgatct
     2701 ttaaagaatg aggatataag ctgtttaaaa cttaattcta attggttaat aggtgaaaaa
     2761 tgaaattagc agaagctctc ttacttcgaa gtgatcaaca aaaaaaaatt atttcactaa
     2821 aacaacgtat caatgcaaat gtattggtac aagatggtga tcaaccatct gaagatccaa
     2881 atgaactact aaaacaagta ttttctttaa ttcaagaatt tcaaaaatta agttatgcaa
     2941 ttcatgaaac taatgcttta acaaaattaa atgatggccg ttctctactt gctttattga
     3001 ctttgcgtga tgaatttgta gaacagcaca aaaccctaac tgcagcaata agtaatacat
     3061 ctcgtgaatc tgatcgttat agtacgcggg aaataaagtg gcataaagtt attccagttt
     3121 catcattaca aaaacaagct gatgatatta gtcttaaatt acgtgattta aacgtgttaa
     3181 ttcagtcgac taattggaaa attgatttga ttgaagccta atagtccttt agatataatc
     3241 aagttgaaca tactgggcga gtacaagatc ccttacttcc acaacatagg attgtaaata
     3301 aagtgtagtg gcttgcaaag caagtggata agcttaaaat tttaagtaca aatcaatcca
     3361 tcacttggca taactcaaat ctttgtgcaa acaaagttac acgttactta taactaccat
     3421 gtactgacag tatgttcacc tcaattacta aaaataaaga aatgaacaag catcagctca
     3481 actacatttc tatttttaat tcagttcggc atttaacata aaatctctta tacgaagccc
     3541 aggttaattc tgggcttttt gttcaatctc atcattggcc gagaacatac ttggattcgt
     3601 aagcctgata ttttcctgac aacttccaca cagcatcaat cctgcaaaaa gttgtacatt
     3661 ttgtctattt tcacaaagcg tacatggctt tgaaagctga ataatattat ctgacactac
     3721 tttaccctct tatagttatc atctgcatat gaataacctt tagcttgtat cattctaacc
     3781 tcatctttag aagcgccatt tttcattaag agccacaaca tcatgaaata tagtccagaa
     3841 aagtcttcat agtatttttc agaaaaattg ttatcttcct caaaatcatc tattcgcttt
     3901 tctaatcgat tcaccagatc ctcaatacga tcggcttgct gtttaacagc aatagattga
     3961 aaatcattgg tcaaaatttt actgaataat ttactggttc tgattccttc tgaattagcc
     4021 aattgatcgt agaattctgc tacttgtgga tctaacctga ctgcataagt tttgactttt
     4081 tcgcccataa attacacctc ttattaaaat tagattttgt acaaaattaa atatatataa
     4141 ttcaactaat taaataaaaa ataataatta gattacgtac aaacttattt gtacagaatt
     4201 aatttattct aaaacaaaaa cttatttaaa tataatgctt tttttatgta caatcatgag
     4261 tacatatttt ttcttaaatt tatcaatatc ttatctaaaa aaagtgttgt tttgatgtac
     4321 aaccatgagc acagcataaa aaatagataa ttcaatcact tattctaaaa aaagaccatt
     4381 tttctgtaca cgtttctgag accctaaaaa atcgctgaaa tccttatcct gtaaggcttt
     4441 gaggggtgtc atcgttagat gcgtggtgtg tgacaaaaaa atcccggcat gtgccggatc
     4501 ttggattgga aaattggcta aagtgacgta gggccggtgc ttggttttgc atggaaaaaa
     4561 gtatttattt ccttgtttag aaaaacgtaa aaaatcaatt ttttgtccat tcatccaggt
     4621 taaaaatttc aacccaaaac tttaattatg aaaagcttca tagaacgcgt tcaaatgcga
     4681 tttaagagcc tttacctaga aaacatagat ctcatagtga aaaacacaaa acagctcaaa
     4741 aaacgcaaat gagagcgaaa taaagaggta ttttgacttt agatagatgc atagagcgag
     4801 tgtctacgag cgaactatca aaatttgcgc ctagaccctc tgaaaaacat ttttttgtcc
     4861 tctttagcct aagaaagcgt aagtttcgtg cagaaatttg ctcctggatc gagcgtagcg
     4921 agtaaaaaag ttttatgagc gaagcgaatt ccaagtttct tttgcttttt tttaaagtca
     4981 cgccagtatt aaccaaaaaa ttgccccaac gaactgagcg aaagcgaagt tcaatagagt
     5041 ttgagcgaag cgaaaaccaa gggcaatttt tcattccctg ggcttttaat tattttaaag
     5101 tttttaaata cttttaaatg gcttgaaagc cttatgaata aagggtttta gctcttatat
     5161 gtccacgttt accttgcaat atgtccacgt ttaccttgca atatgtccac gtttaccttg
     5221 caatatgtcc acgtttacct tgcattagta cacaaataat attaacgtgt acttatacac
     5281 aataaaaaat agtggctatg agagatttag ttgtaaagga caatgcctta atcaacgcaa
     5341 gctataactt agacttagta gaacaacgtt taattttatt ggctattgtt gaagcaaggg
     5401 aaagtgggaa agggattaat gcaaatgatc cattagaagt tcatgcagat agttatatca
     5461 atcaatttgg tgtacaccgt aatacagctt atcaagcctt aaaagatgct tgtaaggatt
     5521 tattcgcgcg tcaatttagc tatcaagaga aaaaagctaa tgggaatatc cgaaatgtta
     5581 tgagtcgttg ggtatctcaa attgcttata acgacaatga agcaactgta gacttaatat
     5641 ttgcacctgc tgttgttccc ttcataaccc gactggaaga acaatttact aaatatgaat
     5701 tacagcaagt tagtagtctt agtagcgctt atgccattcg cttatatgag cttctaattc
     5761 agtggcgaag cactggtaaa accccaacca tagaactaca agaatttaga aagaagttag
     5821 gcgttcttga taatgaatat ttacggatgg ctcatttaaa agagcgtgtt ttagagcttt
     5881 caattaaaca aataaatgag catacggata taactgtaaa atatgaacag cataaaagag
     5941 gacgttctat ttcaggattt tcttttacct ttaaacagaa gaagaaggat agcccatcaa
     6001 tagaaagaga tccgaacact ttggagcttt tttcaaagat gaccgatgct caacggcata
     6061 tgtttgcaaa taaactttca gaactccctg aaatgggtcg ctattcacaa ggaacagaaa
     6121 gctaccaaca gtttgctgta cgtattgctg agatgctaca agatcccgct caattcaaag
     6181 agctataccc atacctaaaa aaagtgggat acatgccatc aaataaaaag gacaccgtaa
     6241 atggctaagt tatcactaag tgaagtatct aaaaaatttc atgtggatag atcaaccatt
     6301 tacagagctg tacgtaatgg acgtttatca cgctccagtg atggacaatt cgatctagca
     6361 gaggtcatac gatgctttgg agaacctgag caaacatctc aaaaaattga atcatctaag
     6421 caagaaggtg atgaatctac aaaaaaactt attgcccatt tagaaaatga agtcaaaaaa
     6481 taccaagaac gtgaagaacg gttaatgcaa caaattgacc gtatgcaaac actcattgag
     6541 ctgaaaagtg ttgcacctgc cacagcagca ccacaccaag atgctacggc atgcgacacc
     6601 aagatgccac agcatgcgac aacacaacaa gacactgata acaaaaagaa taatgaatta
     6661 aatattgcag aaaatgtagc agtgccacag caagaaacta cggcatacca cacccaaacg
     6721 ctacagcatg ccacgttgca aagtgtggca gtgccacaac acaaaaaacg tggtttattt
     6781 ggccgtgtgc tgaatgccgt ctttgataat gactgagcaa taagggtgaa agatcatgcc
     6841 gaaactgaaa gacattgccc tgggaattat tgtagctccg ctgctgatcc cgatcatgct
     6901 gattgcatcg taccaggata aaaaggcgct gaagaaagag ctggatgaat gccaaaaaga
     6961 aaaagaccag gcatcctgat tattcaattg agctaatcaa aagtgtggaa cattgatgaa
     7021 ataatagcgt ggaacgtgaa aagtcataaa aaaagcccga acagggatgc tcgggctata
     7081 aactagaaac tacagcgttg aattataagg ataattatac agcatttcgt ataaggtgta
     7141 ttatgttaat tttaggaaat attaaaacta attttcagac ttatcccacc aactttgagc
     7201 taaatcagta tttttaggaa tacctatacc atcacgatac ataacggaaa gacttcgcat
     7261 tgctttctta ttacctaact ttgcagcctt ttcacgaagt tcaaaagctt tattaatatt
     7321 aacttctatt ccgctatctg cacgagtata tagatcagct aactcaacca ttgattcaga
     7381 atggccattt tctgaggctt tctgaaacca cttaaaagct ttttggaaat gttctaaaga
     7441 atcgtttttg tcatcatctt ccattaatct agcataagta acatatccat cagctaacca
     7501 aaacattgct gctacatttc cattttctgc tgatttaaga gcccaaactt cagcttgatt
     7561 ttcatctgaa tcaacaccat gaccatatag ataaatctcg gctaattcta actgagcaga
     7621 agtatctcca ttttctgctt tcgctaataa atcattagaa aaaacaggtt ctgcaaatgt
     7681 ttccattgaa aggcaaactc ctaaagccat aaaaatattt gttaatttat tcaattttta
     7741 acctttaaac taaactgtta ggcttgtata tttttatatc atacctaaaa gaaaactcta
     7801 aaattagagt atgtcttatc tctatttaac ataatggcga ttatacgaag tacacttgta
     7861 tattaatata aatatcaact acttaataaa aatagaaaaa tcccaaaatg cccaaaaagc
     7921 cgactttgaa acaagtcggc ttttttatga gcagttttga tacttcttgc caataatttt
     7981 gactaaaaat tacacgaatc tggttgagtg atacaatttt gtgatacatt attgtatata
     8041 caaatttgaa tgttttttat ggaacagtat tttgaatggg atgaggctaa gaatcgaaag
     8101 aatcagaaaa agcatgatat ctctttcgaa actgcaagcc ttgtttttga agatccatta
     8161 cggatctcaa tccaagacag acataccaat ggtgaagaac gttggcaaac cattggacga
     8221 gttaaaggcg tactaatgtt attggtggct cacaccattt ttgatgaaga tgactgtgaa
     8281 atcatccgaa tcattagtgc aaggcaagtc actaaagcgg agcgaaacaa atatgagcat
     8341 ggttagatac tcacacaaag agctgaatga gaaatttggt gagaagcaag atgctgaaat
     8401 tcaacgcttg cttgctaaag gcacggttcc tgatgatcag ctagatctat cagatattcc
     8461 tgaaattaca gactggagca atgctgtacg ccaaaatcaa ttttaccgcc cagtaaagca
     8521 acaaacttca attcggttag atgctgatgt acttgcttgg ttcaaagcac aaggcaaagg
     8581 ttatcaaaca cgtatgaatg aaattttacg agatgctatg ctaaaagaat taaaaaatca
     8641 tcaataagaa tgggagctta ggctcccatt tttgacttca tttttagggg ggattaccat
     8701 tcaaaaattt aaatagtaga ttttataaat t
//
