LOCUS       NZ_CP027396            18636 bp    DNA     linear   CON 16-JUN-2024
DEFINITION  Escherichia coli O104:H4 strain FDAARGOS_349 plasmid unnamed3.
ACCESSION   NZ_CP027396
VERSION     NZ_CP027396.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06173362
            Assembly: GCF_002983645.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O104:H4
  ORGANISM  Escherichia coli O104:H4
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18636)
  AUTHORS   Minogue,T., Wolcott,M., Wasieloski,L., Aguilar,W., Moore,D.,
            Tallon,L.J., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A.,
            Nagaraj,S., Vavikolanu,K., Vyas,G., Nadendla,S., Aluvathingal,J. and
            Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18636)
  AUTHORS   Minogue,T., Wolcott,M., Wasieloski,L., Aguilar,W., Moore,D.,
            Tallon,L.J., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A.,
            Nagaraj,S., Vavikolanu,K., Vyas,G., Nadendla,S., Aluvathingal,J. and
            Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 2.3.0, HGAP v. 3.0
            Genome Coverage                   :: 485.42x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002983645.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 03:27:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,451
            CDSs (total)                      :: 5,325
            Genes (coding)                    :: 4,983
            CDSs (with protein)               :: 4,983
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 165 of 342
            Pseudo Genes (incomplete)         :: 196 of 342
            Pseudo Genes (internal stop)      :: 66 of 342
            Pseudo Genes (multiple problems)  :: 72 of 342
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP027396.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..18636
                     /organism="Escherichia coli O104:H4"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_349"
                     /serotype="O104:H4"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1038927"
                     /plasmid="unnamed3"
                     /geo_loc_name="Germany"
                     /collection_date="2001"
     gene            <1..283
                     /locus_tag="CEQ27_RS29185"
                     /old_locus_tag="CEQ27_29170"
                     /pseudo=""
     CDS             <1..283
                     /locus_tag="CEQ27_RS29185"
                     /old_locus_tag="CEQ27_29170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /name="transcription termination factor NusG"
     gene            535..1211
                     /locus_tag="CEQ27_RS30930"
                     /old_locus_tag="CEQ27_29180"
                     /pseudo=""
     CDS             535..1211
                     /locus_tag="CEQ27_RS30930"
                     /old_locus_tag="CEQ27_29180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /name="conjugal transfer protein TraC"
     gene            1339..1628
                     /locus_tag="CEQ27_RS29200"
                     /old_locus_tag="CEQ27_29185"
                     /pseudo=""
     CDS             1339..1628
                     /locus_tag="CEQ27_RS29200"
                     /old_locus_tag="CEQ27_29185"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /name="PilI type IV pilus biogenesis protein"
     gene            2623..3691
                     /locus_tag="CEQ27_RS29215"
                     /old_locus_tag="CEQ27_29200"
                     /pseudo=""
     CDS             2623..3691
                     /locus_tag="CEQ27_RS29215"
                     /old_locus_tag="CEQ27_29200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /name="TcpQ domain-containing protein"
     gene            5757..5879
                     /locus_tag="CEQ27_RS30770"
     CDS             5757..5879
                     /locus_tag="CEQ27_RS30770"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_283938987.1"
                     /translation="MADEDINPVVLLADPKVNHRVWAACLKWTPVVKNSASLTS"
                     /name="hypothetical protein"
     gene            6456..6755
                     /gene="pilO2"
                     /locus_tag="CEQ27_RS30585"
     CDS             6456..6755
                     /gene="pilO2"
                     /locus_tag="CEQ27_RS30585"
                     /inference="COORDINATES: protein motif:HMM:NF018562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="WP_249923302.1"
                     /translation="MASQPVISDFLKACADLRKPSPVALGRLETYRWKCTPETFTLIYE
                     RQPGGTIEGFLAEAKIFNVIPDFNLKDGARLASVTRLLPSLPRRDEAVPAPQNN"
                     /name="pilO2"
     gene            7313..7438
                     /gene="pilP"
                     /locus_tag="CEQ27_RS30590"
     CDS             7313..7438
                     /gene="pilP"
                     /locus_tag="CEQ27_RS30590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03021.1"
                     /GO_process="GO:0009297 - pilus assembly [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="WP_249923303.1"
                     /translation="MADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTSE"
                     /name="pilP"
     gene            7999..8205
                     /locus_tag="CEQ27_RS30775"
     CDS             7999..8205
                     /locus_tag="CEQ27_RS30775"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="WP_283938988.1"
                     /translation="MRLIPDDGDKVPTFKQLGFIPEQIRLLNVMLRRPEGKIVLSGPTG
                     SGKSTTLRSACRVYLTIIRGDTC"
                     /name="ATPase, T2SS/T4P/T4SS family"
     gene            8321..8614
                     /locus_tag="CEQ27_RS30780"
     CDS             8321..8614
                     /locus_tag="CEQ27_RS30780"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="WP_349636727.1"
                     /translation="MRLDPDAIMEGEMRDLVSMMSTTYAAQTGHIVLTTLHTNSALGIP
                     ERMITMGMNADLICDAQLLIGMITSVLYHSVSSCRIPWKSEYLNLAKTTALP"
                     /name="ATPase, T2SS/T4P/T4SS family"
     gene            complement(9846..10082)
                     /locus_tag="CEQ27_RS29255"
                     /old_locus_tag="CEQ27_29240"
     CDS             complement(9846..10082)
                     /locus_tag="CEQ27_RS29255"
                     /old_locus_tag="CEQ27_29240"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_143182668.1"
                     /translation="MGMNADLICDAQLLIGMISQRLVPTLVPPAVFHGKSEYLNLAKTT
                     ALTLSGTAIKIHSAVQIISGSEILTDAPTVITT"
                     /name="hypothetical protein"
     gene            complement(10132..10530)
                     /locus_tag="CEQ27_RS29260"
                     /old_locus_tag="CEQ27_29245"
     CDS             complement(10132..10530)
                     /locus_tag="CEQ27_RS29260"
                     /old_locus_tag="CEQ27_29245"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="WP_105887111.1"
                     /translation="MTETKFPRLSNWALYRSKSVAERHAAPPEGKIVLSGPTGSGNQQR
                     YAVPAVYILTISGRHLLTIEDPLEGQILGATQTPIICDKSDEEAVKLAWSRAISSAMRL
                     DPMPSWKGNARFVSMMSQPTRLRPVTSF"
                     /name="ATPase, T2SS/T4P/T4SS family"
     gene            complement(11105..11558)
                     /gene="pilP"
                     /locus_tag="CEQ27_RS30935"
                     /old_locus_tag="CEQ27_29260"
                     /pseudo=""
     CDS             complement(11105..11558)
                     /gene="pilP"
                     /locus_tag="CEQ27_RS30935"
                     /old_locus_tag="CEQ27_29260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /GO_process="GO:0009297 - pilus assembly [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /name="pilP"
     gene            complement(11545..12828)
                     /gene="pilO2"
                     /locus_tag="CEQ27_RS30785"
                     /old_locus_tag="CEQ27_29265"
                     /pseudo=""
     CDS             complement(11545..12828)
                     /gene="pilO2"
                     /locus_tag="CEQ27_RS30785"
                     /old_locus_tag="CEQ27_29265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /name="pilO2"
     gene            complement(12821..14493)
                     /locus_tag="CEQ27_RS30940"
                     /old_locus_tag="CEQ27_29270"
                     /pseudo=""
     CDS             complement(12821..14493)
                     /locus_tag="CEQ27_RS30940"
                     /old_locus_tag="CEQ27_29270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0009297 - pilus assembly [Evidence IEA];
                     GO:0009306 - protein secretion [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /name="PilN family type IVB pilus formation outer membrane
                     protein"
     gene            complement(14631..14942)
                     /gene="pilM"
                     /locus_tag="CEQ27_RS29290"
                     /old_locus_tag="CEQ27_29275"
     CDS             complement(14631..14942)
                     /gene="pilM"
                     /locus_tag="CEQ27_RS29290"
                     /old_locus_tag="CEQ27_29275"
                     /inference="COORDINATES: protein motif:HMM:NF019064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="WP_249923309.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQKMSETTNAQQYASASVWASQILM
                     IANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWYGCLNNQDLLKLCV"
                     /name="pilM"
     gene            complement(14942..16000)
                     /gene="pilL"
                     /locus_tag="CEQ27_RS29295"
                     /old_locus_tag="CEQ27_29280"
                     /pseudo=""
     CDS             complement(14942..16000)
                     /gene="pilL"
                     /locus_tag="CEQ27_RS29295"
                     /old_locus_tag="CEQ27_29280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /name="pilL"
     gene            complement(<16340..16904)
                     /locus_tag="CEQ27_RS30945"
                     /pseudo=""
     CDS             complement(<16340..16904)
                     /locus_tag="CEQ27_RS30945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334619.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /name="pili assembly chaperone"
     gene            complement(16894..17220)
                     /locus_tag="CEQ27_RS29305"
                     /old_locus_tag="CEQ27_29290"
     CDS             complement(16894..17220)
                     /locus_tag="CEQ27_RS29305"
                     /old_locus_tag="CEQ27_29290"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="WP_349636728.1"
                     /translation="MQRRLFSTKTPTDPDELARRFCTPDNCLVGTTRQRFLFRLERAPQ
                     PLPWHKAAAPAISIYRTGYMNATEAKCYSMVCDDQELHFLFQRIKQLILLSLLFSRLPS
                     QHEK"
                     /name="PilI type IV pilus biogenesis protein"
     gene            complement(17422..18103)
                     /locus_tag="CEQ27_RS29310"
                     /old_locus_tag="CEQ27_29295"
                     /pseudo=""
     CDS             complement(17422..18103)
                     /locus_tag="CEQ27_RS29310"
                     /old_locus_tag="CEQ27_29295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /name="conjugal transfer protein TraC"
     gene            complement(18351..>18636)
                     /locus_tag="CEQ27_RS30950"
                     /old_locus_tag="CEQ27_29300"
                     /pseudo=""
     CDS             complement(18351..>18636)
                     /locus_tag="CEQ27_RS30950"
                     /old_locus_tag="CEQ27_29300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="frameshifted; incomplete; too short partial abutting
                     assembly gap; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /name="transcription termination factor NusG"
ORIGIN
        1 tagcgccttc attgatttcg tgaagtttgg cggtgaaata aaacccgtca ataaagatat
       61 tgtagatgga ttaatgaaga tttacccgga ccctgtgcta aatccgggag ccagggaaga
      121 gctgaatgcg gcttccagca tatggctgac aaaagcacaa tatcagtatc tcctcagaat
      181 ggaaaatact ctccagcctg agtcgaggat atccctgctc ctggaactgg tatcaaatgc
      241 cgaacatcac ggctttatgg agcgccttgt aaacatccca tagccagtat ttcccggcct
      301 tcggccgggc ttttttcaat tcaagagaat attccggagt gaagcctgca cgaagccatc
      361 cccaccatgg actccccctg tcttttgatg gaaccgtgta tttcttaatg gcgaaagaaa
      421 aaaaggcttc accccagagt attgattcgt tttatcaaag gccggcccgt gccatcacgt
      481 gtggcggaga gaacggatac cggtcttctt ttcatctcac agtaggacat tcgcatgacc
      541 acgacagaac atcccgtgcg ccagttgctc tggtgtgcac tgaatgccct gaaaacggca
      601 caggaaaacc acaatataac cagtgaaacc ggattacggc attacctgct tgaatggctt
      661 tctggtgcca gcagacatcc tgagttcagt agtctccctg gcgaaatcat ggcgttaaaa
      721 gcattgaccg aaaaagatcg caatattccc atcatcggaa cgctgaatac gctctttctc
      781 agctctgcac tgtcggggaa tgtccgttgt ttcgttttcg ggctgcgctg ggtaaattac
      841 agaaagcagg ctggcgcact tatgtctgcc catggcctga gatggtgttc aatgaatcca
      901 ttgaacgggc ctgttccggg aaacgccatc tgctacaact aagtcgcacg aagagtgttt
      961 tctaccgaca ggcgatatga ggcaccagtt acgttgcagc tgatcactcc actacaccgc
     1021 aaactatgca gcttctggaa acggcagagt taatattctc cgacgaagga tttcaggtgg
     1081 tgagagatac gaacatcaat tcgggatcga acgcagagaa accctctttc acaccctatt
     1141 tatcgggctg cccagcttgc cagagattct ggggtgcaac acaggagcag acgactatca
     1201 cattacactg aggatgtgta atccccggca taccccgttt ccagtcttcg cagcctgtac
     1261 cggcccttcc tgccgtttcc gacattcttt ctgtcactac tcgcttgtgc cgttttttcg
     1321 caaccggtac gtgcagttat gacggtacag ctgcttcttt cccgttccgg aagagtcttt
     1381 gatgccacag caacatccgg acgactgcaa atactggtcg tcgacgccac tgcaaacgca
     1441 ggctgttttc acgaaaacac cgacagaccc ccgatgagct tgccgacgtt ctgcacgccg
     1501 gataactgtc tcgtggtggt actacgcgac aaccgatttc tgtccgactg gagcgtgctc
     1561 ccggcagcca ctgccgctgg cacaagggca gcagctccgc catcagcatc tacaggactg
     1621 cttcatgacg caaccgaagc aaaatgttat tcatggtgtg tgatgatcag gaactgcatt
     1681 tttttgttcc aaagaattaa acagttaatc ctccttagtc tgttatttag tcgcctgcct
     1741 cagccaacat gaaaaataat aacgcaccat tatatctctg gttgctttgg tgtacgcttt
     1801 gtctgtacat cactacccca tggcatactt acgttatcca tacactgaca acttatcatt
     1861 gcttatatgt tatattctgt gtggtggttg tgatttgcat tttcccacta ctttcagatg
     1921 tgtacttctt attgtaaggt ctgcatctaa atacgacttc acattctttg tgaagaagaa
     1981 aaagggaagc aagaaatcaa ctatacatct tagccctatg ctgaccacgt gccgaagtca
     2041 atgaggaaac actgggatgt attgctttct ctgatatcaa aagcaattaa taatgatgaa
     2101 atcattgtca tgcattcaca tctctttact ccagcaagaa taataaactt taaagaactg
     2161 aatcttatga tttgagtttt tcagttatta catacccacg gaaaacaagt ctgtacgaaa
     2221 aattatcaat tcctttatat atctcttgtt tcagtggtct ttcccgcatt tgcattcgac
     2281 ggaatgacag tcgtcattcg tcctctccag acccaccaca ccaattaaaa atacatttag
     2341 atataaaaag aatgctcata agcaggattt attttcacaa aaaatcccaa aacactggct
     2401 taattgctat ccacaacctc accttaaaat aacatgcaac attaaaattc aaaagataga
     2461 aaatgaaata ctaatcaaca tattaaagac ccaagcagac ttctgaatgc gactaaaacg
     2521 ggacattcag gagtaatctc tcaaaccggt atacattacc catccaatgc acataaaata
     2581 tgaaccctta cttaaagccc gcattaacta acaaacacgt atatacgaac acggagtctc
     2641 cgttatgaaa aaaactatct gacacttata ctgccgtcat tattgtccgg ctgtgccgga
     2701 attccggcgc aataacgggc accatcagat acgcctgctc tggttttgtt gacggacaaa
     2761 tcagtgagag tgctggcgtc ctggcactta cgcaacaacg actttctcca cctaaaataa
     2821 ctccgccccg agagtcagcg tgccagagac agtaaaaaca gcacagccag taagcgaatc
     2881 ccgaccgtta ccttcaccaa caataagtca gaatttctcc gtaatcatta caggcaagcc
     2941 tggtagcacc ctgtaacact atcgccgacg cccgtaaccg gaccgtcagt cagtggataa
     3001 aaacattact accggaagga tggaaattca gacaagagaa tgcagccacg cccaaactga
     3061 atacaaactg gtttcatggt ctgccaatga tcagtggacc cgttctctga accggttgct
     3121 ggaagaacaa cacctctggg ccatctggac tgggataata aaacgttgac cgtcacaaca
     3181 tcggctgctg caccggcaca ggacacggca accactatct cgccagcagc catggtaaca
     3241 catccacttc cccggagtct tcaacgacag caaatagcca gaacaaacca cgaaacccat
     3301 tcagggaaac agcgtatctc cgtctacgcc cgcagcaatc ccgactccga ccgctctaca
     3361 gtcaagtcta tccactcatg accgggacac ctgtcaaacc ggtatctcag ggtaaagagt
     3421 ggcgtgcgcc ggcaggcact acataaggga aacattatta agtgggcaga agaaacaaaa
     3481 tgcgaaagca tggcatcaac gcactggatg gtcatatggc cacttctgta accgattacc
     3541 gtcttgatgc gcctttgatg ttccggggat catttgaatc agccatggag caggtatttg
     3601 agttatatcg gactgcgcaa aacctttata cgcacaggct tctcgcatgc agtgcatcat
     3661 tacagtttct gatacaaggg atggtcgcta atgggatggc tggtcatgcc gtcggcctga
     3721 ccattctgat tatcactggt agctaccaga atcagaagat gtctgaaacc acaaacgccc
     3781 agcaatatgc aagtgcgtct gtatgggcaa gtcagatact aatgattgct aacagaatca
     3841 atgatatcag atacgtatca ggccagcagg acgagtaatt cctcagataa actggctctc
     3901 cctgtcactc cggactcccg aattaaacat cagttacaac agggcgtctg tggtatggat
     3961 gcctgaacaa ccaggacttg ttgaaacttt gcgtttaaaa agtcgtggtt ctgcactaat
     4021 aggatttcca aaacgggcac tcacctggca tcaggaactg caaccggtct gacacccccg
     4081 gcaggcatta ctgctggttc tgtcgtctac gttaactaat ctggatatat tcatgaaaaa
     4141 tcacaccagc gttcaatgaa gctggcgtgc tcccctgcat gatcgccgtc gctcttccat
     4201 ctccggatgt actttcagcg aaatcaacaa atgcagaaaa aagcacagga agactcagca
     4261 catgcacggg aaaaggtatc agccctttcg gcccgtaaat cgcaggctct tactggctcg
     4321 ataatcaatg gataaccctg ttccggtcgc tcaggtatca agagagaaaa aacaaagctc
     4381 agcctgctac atcacgcagg caagaaagga gagatcactc tgcaggaact gggcaacgta
     4441 ttactgccgt atgcggcatc cctgtgatca tcacgcctga cgcagccaat tcaactcttg
     4501 aaggaggcct accgccagat acaggacact accagaccag atgaaaatgg acgtctaccg
     4561 ttaagcagcc tggcagcaca acaatgacta cctccactca gccattaacg ctgaataacc
     4621 tcatgtgcag ggagatatca atggtctctg gatctgatgg ccagccggag tggtctgtac
     4681 tggcgcatgg ataatggtcg gattgtattc tatctgactg aaaccagaac gtatccactt
     4741 catatgctga acaccaaacc agcagcagtt ccagtgtcag ctctggctca acaagcacaa
     4801 tgggggcaac aggagccagg ataactcagc atccggtgat gcaacgtcct ctcagagcaa
     4861 accgtgtcag gaatacgatc tgtatgagat atccggaaaa ctattgaagc aatgctgaca
     4921 ccagaaaagg ccgttactgg tttctgcatc gagctcaacg ctgacagtca ctgatactcc
     4981 agctgtcagg aagccgtcac acgatattag acgaacaaac agtattatga accgccaggt
     5041 agccctgaac gtacaggttc tgagcgtcag caataccaga aacgaacagt tcggtctgga
     5101 ctggaacttg tttataaatc gtacattccg ccggagcaac gttgaacaat gcaagcggag
     5161 atttacaggc gctacatctg caggcgtatc aatctggata cggcaacagg aatgccgcca
     5221 aatcagcggt tccagccttc tgattaaagc atgagtgaac aaggcgatgt cgcgtgtaac
     5281 ttcacaagaa agcactgtca caacctgacg ccggtaccta tccagatggc agatcagaca
     5341 gtttacgtcg ccagtcagca acaactacga ctacggatgt aggagcaaca acaacattaa
     5401 caccggcatg atcaccactg gattcaatat gacctgctgc cttaattcag aaaacaggca
     5461 atctccagtt gcagatgaac tttaatctgt ctgatccccc aactatccgt actttacgtc
     5521 aaaagacgga aacagttaca tcgaaatgcc atataccaac tgcgttcact gaccagaagg
     5581 tcaatcgaaa gaagggcaat cacttgtcgt tactggtttc gatcagaaca atacgacgac
     5641 aagtaaagcc ggtacattta cgccagcaaa tccattattg gtggttcaca aaccgggaaa
     5701 atgaacgcag cacgcttgtg atcatcatta ccccgacttt ccctcaggag gcaataatgg
     5761 ctgatgaaga tatcaacccg gtagtgcttc tcgcagatcc taaagtaaat catcgcgtgt
     5821 gggctgcctg tcttaaatgg actcctgtcg taaaaaacag cgcgtccctc acatcataaa
     5881 caagtatcat gtaaacccag acggcttacc tccctgaaag tgactgtagg cagcagaaat
     5941 tcccgtggaa agatcagccg ccttacgcac aggaatactg accagccaga acgcaatcat
     6001 tatttttccc tggcactggc tttctgctca tgggtcagaa atggatacgg tgtcttccgg
     6061 tacagcgata aggagctctt attcctgctt ccattaatgg ccagcctgcc gttatggctg
     6121 acttatcagg aaatgatgcc gatgttgccc aaaagtcagc cttttctgcg atgaacgaag
     6181 agccccccga aaaatggcag gttgatcgtc ttggaacatc ctgataactg ggaaagtatt
     6241 attacccggt tatcatcagc agacctccgc gttgcaactt actgtcgcaa tcgcagcaaa
     6301 ttcacactgc ctgccgtact gttttttagc agccggcgca ggcacgtatt ctggatgact
     6361 cagccagagc ctgatgtcgg accgaccgcc gaggagtagc tgcacgggca agattacagt
     6421 ttaaaagccc gaaccaccac cggagctccg catccatggc gtcacaacct gtaatcagtg
     6481 attttctgaa agcctgcgcg gaccttcgaa aaccatcacc ggtcgcgcta ggaaggctgg
     6541 aaacttaccg gtggaaatgt actcccgaga cattcacact gatttacgaa cgacagccag
     6601 gaggaactat tgaaggcttt ctggcagaag caaaaatctt taatgttatt cctgacttta
     6661 accttaaaga tggtgcacgc ctggcttctg taacccggct actgccttcc ctgccccgca
     6721 gggatgaggc agtccctgcc cctcagaaca actgatgcgg gtgttacatg gtttcagaaa
     6781 aacagttaac gcggccatta atgaaatagc cataccggaa ccattaccag gtaacaatgg
     6841 tgaaccagcc ctgttcagaa tggaaagaat atcagttcag tttatcacgc ctgtaatcct
     6901 gatgcgttgt tccctttatt tcaggatacc ggcgttcgtc tgagcaatat ccatttgaat
     6961 taaacggcgg tactttcagt tattcctcag aaggtcatat ttatgcgtcc aggtaaatca
     7021 ctcattatcc atccatattt tcttctccgg tttcatttgc aacaacacaa ctcttgtcac
     7081 aataggagaa cttgaagcac aacaaacgga tatttttaca ggcaaaattc agggcgcaca
     7141 gctacagaaa caactcgaag aatcaatgtt aactgtcctc tgaaactgtt caggtttctc
     7201 tggtatgact gcctctttgc ctcagtttca gagcagccga catctaaaac agaaaggaat
     7261 tgcccgttat atggaataaa cgcaaagata aacgtcttca tgcgttcttc gcatggctga
     7321 tggacgtcag acaagcgtca ccactggtag ccagcttccg ggtacatccg tcacggtgaa
     7381 gtcgatctca ttgtctggcg tcacgttaag tgatggcacc acccttactt ctgagtgatg
     7441 tgatatggac aaatataatt tgaaggatgc tctccttttt atatcccggg agacacccat
     7501 gaattctcat cgaaacaaat cagcgaacaa gacctgacgt acagagtaat ctccagaact
     7561 gctaaactta taccctgaat catccaaaag tagtctcgct gtctgagttc aggaagccgg
     7621 tcaggatgat gaaaataaag gtccgaaaaa acggattatt catctgaaag atcttgcaga
     7681 tgtcagtgaa agtgaaagaa agttctctcc tattttgaaa cggccagaac tggtgcttca
     7741 gatattcatt tttaatttca gagtctattt caaattcgca tgagaatatc ggtgaactgc
     7801 aaactgtaga tgaagatcaa cctgcgtggg ttattcactt tgcgcaacag ccattctgtc
     7861 aatggctgat gtgactgaaa ccagtttctt ccctcaaagg gaacaggatg cgcgcctgtc
     7921 cccacaatta atgcgaaaat aggaatattt ggtgcacgat acagtcacag acctacggga
     7981 gatggtctga ttgcagttat gcgcctgata cccgatgacg gagacaaagt tcccacgttt
     8041 aagcaactgg gctttatacc ggagcaaatc cggttgctga acgtcatgct gcgccgcccg
     8101 gaagggaaaa tagttctgtc tggtccaaca ggttcaggta aatcaacaac gttacgcagt
     8161 gcctgccgtg tatatctgac gataatcagg ggcgacacct gctgacgatc gaagatccgc
     8221 tggaaggaca gattctgggg caacacaaac cccgattatc tgtgacaaat ccgacgaaga
     8281 ggccgtcaaa ctggcatgga gtcgtgctat ttcatcggca atgcgacttg accccgatgc
     8341 catcatggaa ggggaaatgc gcgatcttgt ctcgatgatg tcaacaacct acgcggctca
     8401 gaccggtcac atcgttctga cgacattaca tacaaactca gctctgggta taccggagcg
     8461 gatgattacg atgggtatga atgccgatct tatttgcgat gcgcaattac tgattgggat
     8521 gatcaccagc gtcttgtacc actctgtgtc ctcctgccgt attccatgga aaagcgagta
     8581 cctgaactta gcgaagacga ccgcgctacc ttgagcggca ctgcaataaa gattcactct
     8641 gcagtacaga taatatctgg ttccgaaatc ctcacggatg ctccgactgt aatcacgacg
     8701 taattattaa cggcagaaag cgcggtgaaa taggtaaagg cctcaccggc aggaccgtaa
     8761 ttgctgaagt gatagaacct gataatcgct tatttcagat tctgaaaaca cgcggcaaag
     8821 ttgcagcacg taaatactgg cttgaaaaca tgcatggcat cagtcggtag agcatcttct
     8881 tcgccgtatc aatgaaggcc ggtggaccct cttgaaggcc gacagaatca ctccctctgg
     8941 acgaagacga acgcctcagc atcgatgatg cctaacagga gaacaagcat ggccgtgatg
     9001 aatgttcagt caacgccacc acacttatca gatgccaaag cagactccac aggcataggc
     9061 aaatggctat ttacccggtg gggttaatca tgatgacggg gacgatatat ttgctcgtac
     9121 aggtttagct tcgtattaag aacatataat gtccccgatc atcatgatta aaacaagacc
     9181 ggtgaaaagc catttgcata tggcctgatg gagttgccgt ttggcatcgg tagtgtggtg
     9241 gcaattgtaa tgcatccaca atgaaccact tttattcgca ctgattactg acctctttcc
     9301 tgggaaccca ttaaaccaag tagtatattc cagagagttt tcgccactgt ttttcacgaa
     9361 gagactcaat acatctgtgg ccagttcagc aaagggatgc cattttcgtc aaagtctgtc
     9421 caggcatccc gcatctgttc cagtgcgtct ttaatggctg tttattttcc agcagaaaac
     9481 gcagagcttc gtaaactgca cctgtagggg gcgctgaacg tttttctgac tgaaacgtct
     9541 cagccgtgat gaaattcatt tcacgcatgt tgttctctgt taggcatcat cgatgctgag
     9601 gcgttcgtct tcgtccagag ggatgattct gtcggcttca agaggtccac caggccttca
     9661 ttgatacggc gaagaagatg ctctacacga ctgatgccat gcatgttttc aagccagtat
     9721 ttacgtgctg caactttgcc gcgtgttttc agaatctgaa taagcgatta tcaggttcta
     9781 tcacttcagc aattacggtc ctgccggtga gcctttacta ttcaccgcgc ttctgccgtt
     9841 aataattacg tcgtgattac agtcggagca tccgtgagga tttcggaacc agatattatc
     9901 tgtactgcag agtgaatctt tattgcagtg ccgctcaagg taagcgcggt cgtcttcgct
     9961 aagttcaggt actcgctttt cccatggaat acggcaggag ggacaagagt tggtacaaga
    10021 cgctggctga tcatcccaat cagtaattgc gcatcgcaaa taagatcggc attcataccc
    10081 atcgtaatca tccgctccgg tatacccaga gctggtttgt atgtaatgtc gtcagaacga
    10141 tgtgaccggt ctgagccgcg taggttgtga catcatcgag acaaatcgcg catttccctt
    10201 ccatgatggc atcgggtcaa gtcgcattgc cgatgaaata gcacgactcc atgccagttt
    10261 gacggcctct tcgtcggatt tgtcacagat aatcggggtt tgtgttgccc ccagaatctg
    10321 tccttccagc ggatcttcga tcgtcagcag gtgtcgccct gatatcgtca agatatacac
    10381 ggcaggcact gcgtaacgtt gttgattacc tgaacctgtt ggaccagaca gaactatttt
    10441 cccttccggc ggcgcagcat gacgttcagc aacggatttg ctccggtata aagcccagtt
    10501 gcttaaacgt gggaactttg tctccgtcat cgggtatcag cgcataactg caatcagacc
    10561 atctcccgta ggtctgtgac tgtatcgtgc accaaatatt cctattttcg cattaattgt
    10621 gggacaggcg cgcatcctgt tcctttgagg gaagaactgg tttcagtcac atcagccatg
    10681 acagaatggc tgttgcgcaa agtgaataac ccaacgcagg ttgatcttca tctacagttt
    10741 gcagttcacc gaatattctc atgcgaactt tgaaatagac tctgaaatta aaaatgaata
    10801 tctgagcacc aagttttctg gccgtttcaa atagagagaa ctttcttctc actttcactg
    10861 acatctgcaa gatctttcag atgaataatc cgttctttcg gaccttattt catcatcctg
    10921 accggcttcc tgtaactcag acagcgagac tactttggga tgatgtcagg gtataagttt
    10981 agcagttcct ggagattact ctgtacgtca ggtcttgttc gctgatttgt ttcgatgaga
    11041 atttcatggg tgtctccccg gatataaaag gagagcatcc ttcaattata ttgtccatat
    11101 cacatcactc agaagtaagg gtggtgccat cacttaacgt gacgccagac aatgagatcg
    11161 acttcaccgt gacggatgta cccggaagct ggctaccagt ggtgacgctt gtctgacgtc
    11221 catcagccat gcgaagaacg gcatgaagac gtttatcttt gccgtttatt tccataataa
    11281 cgggcaattc ctttctgttt tagatgtcgg ctgctctgaa actgatggca aagaggcagt
    11341 cataccagag aaacctgaaa cagtttcaga ggacagatta acatctgatt cttcgagttg
    11401 tttctgtagc tgtgcgccct gaacttttgc ctgtaaaaga atattccggt ttgttgtgct
    11461 tcaagttctc ctattgtgac aagaggttgt gttgttgcaa atgaaaaacc ggagaagaaa
    11521 aatatggatg gaataatgag tgatttacct ggacgcataa atatgacctt ctgaggaata
    11581 actgaaagta ccgccgttta attcaaaatg gatattgctc agacgaacgc cggtatcctg
    11641 aaataaaggg aacaacgcat caggatttac aggcgttgat aaactgaact gatattcttt
    11701 ccatttctga acaggggctg gttcaccatt gttacctggt aatggttccg gtatggctat
    11761 ttcattaatg gccggcgtta actgtttttt ctgaaaccat gtgaacaccc gcatcagttg
    11821 ttctgagggg cagggactgc ctcatccctg cggggcagga aggcagtagc cgggttacag
    11881 aagccaggcg tgcaccatct ttaaggttaa agtcaggaat aacattaaag atttctttgc
    11941 ttcttgccag aaagccttca atagttcctc ctggctgtcg ttcgtaaatc agtgtgaatg
    12001 tctcgggagt acatgttcca ccggtaagtt tccagccttc tagcgcgacc ggtgatggtt
    12061 ttcgaaggtc cgcgcaggct tcagaaatca ctgattacag gttgtgacgc ccatggatgc
    12121 gggagctccg gtggtggttc gggcttttaa ctgtaatctt gcccgtgcag ctatctcctc
    12181 ggcggtcggt ccgacatcag gctctggctg agtcatccag aatactgtgc ctgcgccggc
    12241 tgctactaaa acagtacggc aggcagtgtg aatttgctgc gattgccgac agtaagtttg
    12301 caacggcgga ggtctgctga tgataaccgg gtaataatac tttcccagtt atcaggatgt
    12361 tccagagacg atacaacctg ccatttttcg ggggctcttc gttcatcgcc agaaaaggct
    12421 gaccttttgg gcaacatcgc atcatttcct gataagtcag ccataacggc aggctggcca
    12481 ttaatggaag caaggaataa gagctcttat cgctgtaccg gaagacaccg tatccatttc
    12541 tgacccatga gcagaaagcc agtgccaggg aaaaataatg attgcgttct ggcctggtca
    12601 gtattcctgt gccggtaagg cggctgatct ttccacggga atttctgctg cctacagtca
    12661 ctttcaggga ggtaagccgt ctgggtttta catgatactt gtgtttatga tgtgagggga
    12721 cgcgctgttt tttacgacag gagtccattt aagacaggca gcccacacgc gatgatttac
    12781 tttaggatct gcgagaagca ctaccgggtt gatatcttca tcagccatta ttgcctcctg
    12841 acgggaagtc gggtaatgat gatcacaagc gtgctgcgtt catttttccc ggtttgtgaa
    12901 ccaccaaata atggatttgc tggcgtaaat gtaccggctt tacttgtcgt cgtattgttc
    12961 tgatcgaaac cagtaacgac aagtgattgc ccttctttca gatgaccttc tggctcagtg
    13021 aacgcagttt ggtatatggc atttcgatgt aactgtttcc gtcttttgac gtaaagctac
    13081 ggatagttgg gggatcagac agattaaagt tcatctgcaa ctggagattg cctgttttct
    13141 gaattaaagg cagcagggtc atattgaatc cagtggtgat catgcccggt gttaatgttg
    13201 ttgttgctcc tacatccgta gtcgtagttg ttgctgactg ggcgacgtaa ctgtctgatc
    13261 tgccatctgg ataggtaccg gcgtcaggtt tgtgacagtg ctttcttgtg aagttacaac
    13321 gctgacatcg ccttgttcac tcagtgcttt aatcagaagg ctggaaccgc tgaatttggc
    13381 ggcattcctg ttgccgtatc cagaattgat acgcctgcag atgtagcgcc tgtaaaatct
    13441 ccgcttgcat tgttcaacgt tgctccggcg gaatgtagcg atttataaac aaggttccag
    13501 tccagaccga actgttcgtt tctggtattg ctgacgctca gaacctgtac gttcagggct
    13561 acctggcggt tcataatact gttttgttcg tctacatatc gtgtgacggc ttcctggaca
    13621 gctggagtat cagtgactgt cagcgttgag ctcgatgcag ataaccagta acggcctttt
    13681 tctggtgtca gcattgcttc aatagttttc cggatatctt catacagatc gtattcctga
    13741 ccaacggttg tgctctgaga ggacgttgca tcaccggatg ctgagttatc ctggcctcct
    13801 gttgccccat tgtgcttgtt gagccagagc tgacactgga actgctgctg gttttggtgt
    13861 tcagcatatg aagtggatac gttctggttt cagtcagata gaatacatcc gaccattatc
    13921 catgcgccag tacagaccac tccggctggc catcagatcc agaagaccat tgatatctcc
    13981 ctgccacatg aggttattca gcgttaatgg ctgagtggag gtagtcattg ttgtgctgcc
    14041 aggctgctta acggtagacg tccattttca tctggtgctg gtagtgttcc tgtcatctgg
    14101 cggtagcgcc tccttcaaga gttgaattgg ctgcgtcagg cgtgatgatc acagggatgc
    14161 cgcatacggc agtaatacgt tgccccagtt cctgcagagt gatctctcct ttcttgcctg
    14221 cgtgatgtag caggctggag ctgtttgttt tttctctctt gatacctgag cgaccggaac
    14281 agggtttatc cattgattat cgagccaggt aagagcctgc gatttacggg ccgaaagggc
    14341 tgataccttt tcccgtgcat gtgctgagtc ttcctgtgct tttttctgca ttttgttgat
    14401 ttcgctgaaa gtacatccgg agatggaaag agcgacggcg atcatgcagg ggagcaccgc
    14461 cagcttcatt gaacgctggt gtgatttttt catgaatata tccagattag ttaacgtaga
    14521 cgacagaacc agcagtaatg cctgccgggg gtgtcagacc ggttgcagtt cctgatagcc
    14581 aggtgagttg cccgtttgga agatccctat tagtgcagaa ccacgacttt ttaaacgcaa
    14641 agtttcaaca agtcctggtt gttcaggcat ccataccaca gacgcccctg ttgtaactga
    14701 tgtttaattc gggagtccgg agtgacaggg agagccagtt tatctgagga aattactccg
    14761 tcctgctggc ctgatacgta tctgatatca ttgattctgt tagcaatcat tagtatctga
    14821 cttgcccata cagacgcact tgcatattgc tgggcgtttg tggtttcaga catcttctga
    14881 ttctggtagc taccagtgat aatcagaatg gtcaggccga cggccatgac cagccatccc
    14941 attagcgacc atcccttgta tcagaaactg taatgatgca ctgcatgcga gaagcctgtg
    15001 cgtataaagg ttttgcgcag tccgatataa ctcaaatacc tgctccatgg ctgattcaaa
    15061 tgatccccgg aacatcaaag gcgcatcaag acggtaatcg gttacagaaa gtggccatat
    15121 gaccatccag tgcgttgatg ccatgctttc gcatttgttt cttctgccac ttaataatgt
    15181 tttcccttaa tgtagtgcct gccggcgcac gccactcttt accctgagat accggtttga
    15241 caggtgtccc ggtcatgagt gggatagact tgactgtaga gccggtcgga gtcgggattg
    15301 ctgcgggcgt agacggagat acgctgtttc ccctgaatgg gtttcgtggt ttgttctggc
    15361 tatttgctgt cgttgaagac tccgggaagt ggatgtggtt accatggctg ctggcgagat
    15421 agtggttgcc gtgtcctgtg ccggtgcagc agccgatgtt gtgacggtca acgttttatt
    15481 atcccagtcc agatggccca gaggtgttgt tcttccagca accggttcag agaacgggtc
    15541 cactgatcat tggcagacca tgaaaccagt ttggtattca gtttgggcgt ggctgcattc
    15601 tcttgtctga atttccatcc ttccggtagt aatgttttta tccactgact gacggtccgg
    15661 ttacgggccg tcggcgatag tgttacaggg gtgctaccag gcttgcctgt aatgattaac
    15721 cgggagaaat tctgacttat tgttggtgaa ggtaacggtc gggattcgct tactggctgt
    15781 gctgttttac tgtctctggc acgctgactc tcggggcgga gttattttag gtggagaaag
    15841 tcgttgttgc gtaagtgcca ggacgccagc actctcactg atttgtccgt caacaaaacc
    15901 agagcaggcg tatctgatgg tgccacgtta ttgccgccgg aattccggca cagccggaca
    15961 ataatgacgg cagtataagt gtcagatagt tttttttcat aacggagact ccgtgttcgt
    16021 atatacgtgt ttgttagtta atgcgggctt taagtaaggg ttcattattt atgtgcaatt
    16081 ggatgggtaa tgtataccgg tattgagaga attactcctg aatgtccgtt ttagtcgcat
    16141 tcagaagtct gcttgggtct ttaatattgt tgattagtat tttcattatt ctatcttttg
    16201 aattttaatg ttgcatgtta tttaaaggtg aggttgtgga tagccaatta agccagtgtt
    16261 ttggattttt gtgaaaataa aaatcctgct tatgagcatt ctttttatat ctaaatgtat
    16321 ttttaattgt gtggtgggtc tggagaggac gaatgacgac tgtcattccg tccgaatgca
    16381 aatgcgggaa agaccactga aacaagagat atataaaagg aattgataat tttcgtacag
    16441 acttgttttc cgtgggtatg taataactga aaaactcaaa tcataagatt ccagtctctt
    16501 taaagtttat ttattcttgc tggagtaaag agatgtgaat gcatgacatg atttcatcat
    16561 tattaattgc tttgatatca gagaaagcaa tacatccagt gtttcctcat tgacttcggg
    16621 caacgtgtgt cagcataggg gctaagatgt atagttgatt tcttgcttcc cttttcttct
    16681 tcacaaagaa tgtgaagtcg tatttagatg cagaccttac aataagaagt acacatctga
    16741 aagtagtggg aaaatgcaaa tcacaacacc acacagaaat ataacatata aggcaatgat
    16801 aattgtcagt gtatggataa gtaagtatgc catggggtag tgatgtacag acaaagcgta
    16861 caccaaagca accagagata taatggtgcg ttattatttt tcatgttggc tgggcaggcg
    16921 actaaataac agactaagga ggattaactg tttaattctt tggaacaaaa aatgcagttc
    16981 ctgatcatca cacaccattg aataacattt tgcttcggtt gcgttcatgt agccagtcct
    17041 gtagatgctg atggcgggag ctgctgcctt gtgccacggc agtggctgcg gagcacgctc
    17101 cagtcggaac agaaatcgtt gtcgcgtagt acccacgaga cagttatccg gcgtgcagaa
    17161 acgtcgggca agctcatcgg ggtctgtcgg tgttttcgtt gaaaacagcc tgcgttgcag
    17221 tgtgcgtcga cgaccagtat ttgcagtcgt ccgggatttg ctgtggcatc aaagactctt
    17281 ccggaacggg aaaagaagca gctgtacgtc ataactgacg taccggttgc gaaaaaacgg
    17341 cacaagcgag tagtgacaga agaatgtcgg aaacggcaga agggcggtac agctgcgaga
    17401 ctggaacggg tatgccgggg attacacatc ctcagtgtat gtgatagtcg tctgctctgt
    17461 gttgcaccca gacatcttct ggcaagctgg gcagcccgat aaataggtgt aaagagggtt
    17521 tctctgcgtt cgatcccgaa ttgatgttcg tatcctctca ccacctaatc cttcgtcggg
    17581 aatattactc tgccgtttcc agaagctgca tagtttgcgg tgtagtggag tgatcagctg
    17641 caacgtaact gtgccctcat atcgcctgtc ggtagaaaac actctcggtg cgacttagtt
    17701 gtagcagatg gcgtttcccg gaacaggccc gttcaatgga ttcattgaac accatctcag
    17761 gccatgggca gacataagtg cgccagcctg ctttctgtaa tttacccagc gcagcccgaa
    17821 aacgaaacaa cggacattcc ccgacagtgg cagagctgag aaagagcgta ttcagcgttc
    17881 cgatgatggg aatattgcga tctttttcgg tcaatgcttt taacgccatg atttcgccag
    17941 ggagactact gaactcagga tgtctgctgg caccagaaag ccattcaagc aggtaatgcc
    18001 gtaatccggt ttcactggtt atattgtggt tttcctgtgc cgttttcagg gcattcagtg
    18061 cacaccagag caactggcgc acgggatgtt ctgtcgtggt catgcgaatg tcctactgtg
    18121 agatgaaaga agaccggtat ccgttctctc cgccacacgt gatggcacgg gccggccttt
    18181 tgataaaacg aatcaatact ctggggtgaa gccttttatt tctttcgcca ttaagaaata
    18241 cacggttcca tcaaagacag ggggagtcca tgggggatgg ctcgtgcagg cttcactccg
    18301 gaatattctc tgaatgaaaa aagcccggcc cgaaggccgg gaaatactgg ctatgggatg
    18361 tttacaaggc gctccataaa gccgtgatgt tcggcatttg ataccagttc aggagcaggg
    18421 atatcctcga ctcaggctgg agagtatttt ccattctgag gagatactga tattgtgctt
    18481 ttgtcagcca tatgctggaa gccgcattca gctcttccct ggctcccgga tttagcacag
    18541 ggtccgggta aatcttcatt aatccatcta caatatcttt attgacggtt ttatttcacc
    18601 gccaaacttc acgaaatcaa tgaaggcgct atggcg
//
