LOCUS       NZ_CP027393            12402 bp    DNA     linear   CON 16-JUN-2024
DEFINITION  Escherichia coli O104:H4 strain FDAARGOS_349 plasmid unnamed4.
ACCESSION   NZ_CP027393
VERSION     NZ_CP027393.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06173362
            Assembly: GCF_002983645.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O104:H4
  ORGANISM  Escherichia coli O104:H4
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12402)
  AUTHORS   Minogue,T., Wolcott,M., Wasieloski,L., Aguilar,W., Moore,D.,
            Tallon,L.J., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A.,
            Nagaraj,S., Vavikolanu,K., Vyas,G., Nadendla,S., Aluvathingal,J. and
            Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12402)
  AUTHORS   Minogue,T., Wolcott,M., Wasieloski,L., Aguilar,W., Moore,D.,
            Tallon,L.J., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A.,
            Nagaraj,S., Vavikolanu,K., Vyas,G., Nadendla,S., Aluvathingal,J. and
            Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 2.3.0, HGAP v. 3.0
            Genome Coverage                   :: 485.42x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002983645.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 03:27:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,451
            CDSs (total)                      :: 5,325
            Genes (coding)                    :: 4,983
            CDSs (with protein)               :: 4,983
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 165 of 342
            Pseudo Genes (incomplete)         :: 196 of 342
            Pseudo Genes (internal stop)      :: 66 of 342
            Pseudo Genes (multiple problems)  :: 72 of 342
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP027393.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..12402
                     /organism="Escherichia coli O104:H4"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_349"
                     /serotype="O104:H4"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1038927"
                     /plasmid="unnamed4"
                     /geo_loc_name="Germany"
                     /collection_date="2001"
     gene            <1..747
                     /gene="trbA"
                     /locus_tag="CEQ27_RS29765"
                     /pseudo=""
     CDS             <1..747
                     /gene="trbA"
                     /locus_tag="CEQ27_RS29765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="frameshifted; incomplete; too short partial abutting
                     assembly gap; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /name="trbA"
     gene            766..1833
                     /gene="trbB"
                     /locus_tag="CEQ27_RS30260"
                     /old_locus_tag="CEQ27_00550"
                     /pseudo=""
     CDS             766..1833
                     /gene="trbB"
                     /locus_tag="CEQ27_RS30260"
                     /old_locus_tag="CEQ27_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /name="trbB"
     gene            1826..4113
                     /gene="trbC"
                     /locus_tag="CEQ27_RS30265"
                     /old_locus_tag="CEQ27_00555"
                     /pseudo=""
     CDS             1826..4113
                     /gene="trbC"
                     /locus_tag="CEQ27_RS30265"
                     /old_locus_tag="CEQ27_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /name="trbC"
     gene            complement(4150..6844)
                     /gene="nikB"
                     /locus_tag="CEQ27_RS00560"
                     /old_locus_tag="CEQ27_00560"
                     /pseudo=""
     CDS             complement(4150..6844)
                     /gene="nikB"
                     /locus_tag="CEQ27_RS00560"
                     /old_locus_tag="CEQ27_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /name="nikB"
     gene            complement(6855..7183)
                     /gene="nikA"
                     /locus_tag="CEQ27_RS29770"
                     /pseudo=""
     CDS             complement(6855..7183)
                     /gene="nikA"
                     /locus_tag="CEQ27_RS29770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxosome accessory protein NikA"
                     /name="nikA"
     gene            7414..7745
                     /locus_tag="CEQ27_RS00570"
                     /old_locus_tag="CEQ27_00570"
                     /pseudo=""
     CDS             7414..7745
                     /locus_tag="CEQ27_RS00570"
                     /old_locus_tag="CEQ27_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372351.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /name="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
     gene            complement(7830..8688)
                     /locus_tag="CEQ27_RS30270"
                     /pseudo=""
     CDS             complement(7830..8688)
                     /locus_tag="CEQ27_RS30270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /name="DUF4942 domain-containing protein"
     gene            9018..9217
                     /locus_tag="CEQ27_RS30800"
                     /pseudo=""
     CDS             9018..9217
                     /locus_tag="CEQ27_RS30800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            9245..9495
                     /locus_tag="CEQ27_RS00595"
                     /old_locus_tag="CEQ27_00595"
                     /pseudo=""
     CDS             9245..9495
                     /locus_tag="CEQ27_RS00595"
                     /old_locus_tag="CEQ27_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(9535..9726)
                     /locus_tag="CEQ27_RS00600"
                     /old_locus_tag="CEQ27_00600"
                     /pseudo=""
     CDS             complement(9535..9726)
                     /locus_tag="CEQ27_RS00600"
                     /old_locus_tag="CEQ27_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150316.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(9723..9920)
                     /locus_tag="CEQ27_RS29815"
     CDS             complement(9723..9920)
                     /locus_tag="CEQ27_RS29815"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_192941388.1"
                     /translation="MEGRWKERLKSPGYCVPAQKMGMTPEQIKQATGLSDDELKKSFTD
                     ENLTARQNTGPLPAGAEKTV"
                     /name="hypothetical protein"
     gene            complement(9946..10107)
                     /locus_tag="CEQ27_RS00605"
                     /old_locus_tag="CEQ27_00605"
     CDS             complement(9946..10107)
                     /locus_tag="CEQ27_RS00605"
                     /old_locus_tag="CEQ27_00605"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_249923298.1"
                     /translation="MDNPAGHGEISDEQVRVRLLHRNAGDSVSPEFMRALAERLPQHED
                     KLMTIADS"
                     /name="hypothetical protein"
     gene            <10320..>10514
                     /locus_tag="CEQ27_RS30275"
                     /pseudo=""
     CDS             <10320..>10514
                     /locus_tag="CEQ27_RS30275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038327.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /name="Rpn family recombination-promoting nuclease/putative
                     transposase"
     gene            10575..10781
                     /locus_tag="CEQ27_RS30280"
     CDS             10575..10781
                     /locus_tag="CEQ27_RS30280"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_249923299.1"
                     /translation="MIHYIVTQGLCITGILRALVSVCRSRDKLMTIAERLEQKGRQEGR
                     MEGRMEGALEKALAMRASFRKWG"
                     /name="hypothetical protein"
     gene            10907..11110
                     /locus_tag="CEQ27_RS00615"
                     /old_locus_tag="CEQ27_00615"
     CDS             10907..11110
                     /locus_tag="CEQ27_RS00615"
                     /old_locus_tag="CEQ27_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_105887101.1"
                     /translation="MTIAERLIQKGFDEGFDEGFKEGFKKGALEVRGKQPAAAGYGWTP
                     ERIQEAAGLSGEELKKLFPDEQ"
                     /name="hypothetical protein"
     gene            complement(11141..11390)
                     /locus_tag="CEQ27_RS00620"
                     /old_locus_tag="CEQ27_00620"
                     /pseudo=""
     CDS             complement(11141..11390)
                     /locus_tag="CEQ27_RS00620"
                     /old_locus_tag="CEQ27_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(11434..11619)
                     /locus_tag="CEQ27_RS30805"
                     /old_locus_tag="CEQ27_00625"
     CDS             complement(11434..11619)
                     /locus_tag="CEQ27_RS30805"
                     /old_locus_tag="CEQ27_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_105887102.1"
                     /translation="MQGAPHPARLKKNDGTTMACGGHWHTTPAQVKTEKTARHLQPKKV
                     KFLHFFSLIYRADWQL"
                     /name="hypothetical protein"
     gene            11967..>12402
                     /locus_tag="CEQ27_RS00635"
                     /old_locus_tag="CEQ27_00635"
                     /pseudo=""
     CDS             11967..>12402
                     /locus_tag="CEQ27_RS00635"
                     /old_locus_tag="CEQ27_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /name="DUF4942 domain-containing protein"
     Typing          complement(7185..7284)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.098"
                     /coverage="100.0"
                     /evalue="3.87e-37"
     Typing          complement(5165..5820)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.543"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(5913..6844)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.534"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2609..4080
                     /name="MPF_I"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="87.018"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 catggtggca aacggcctga gcggcgggtt gctctcaccc cggaagcatt tgtcgagcag
       61 catgcactca tcaccaatat gcagctcgat ttgcagctgc agacgatgtt tcatggccca
      121 gtgggtaaac ctttaacgtc ctggaagaca tggcaccaca tgaaaaagcg ctctttgcca
      181 tatttggcct tcagtatttc ctggatgatc gtaaggcagc attaaagctc atggatacac
      241 tgaacctctc atcagaatta aaagcaaacg ggacagcggg aaattctgta cccctgttta
      301 ttcactggcc aaatcagctt tccagagagt gattaaagca acggagcaca gcaatggctg
      361 aaacaacatc gctacgtccg cagtgggctg gtctggctgt atgcacatga cctgcggctt
      421 acgccaccca actggatttg gctgaaaggc gtggacagaa cgctgtttta tgcattacat
      481 ccgcgccaat accacgaaag gatttattga aggcgctggt gtggtggccg ttgcacgtgc
      541 agaggccgaa gccatgcgtt ttggccttcc ctgccctgaa ccctgtgttg atgaagcggt
      601 ggaaggctta cggcgggata tgctcagtct cggtcttatc tgggatgaac cacaaaccag
      661 atcgcgaccg gaacgccgca tcctgaccaa ctggtccctc accgacgaca tcctgcccgt
      721 acacctgcta cagacaacga attctgatta tccggagaac tgaaaatgac aatcgagtac
      781 tttgcaacac gcattgcaaa catatttccg tgcaggcaat ctggccggat ggcaggacag
      841 aaattattgc cgtccttcca cgggatgccc tatcagggct ggtaacacga aataatcaac
      901 tgattcatat agcctttgac gccatgggca cccgtaacag agaaaccgtc ctgattgata
      961 accaggagca taacgctgac cagatactta cagctattga ttcctgcctg cgccttgaat
     1021 acctgatgga acgccgcttc agcagaacgc ctttgttccg tggcataatc gccagcgttg
     1081 tgctgttcgt aatggcaacg attggcttct ctctgttccg ctatgttgat cgtgtattct
     1141 gggatgatac aacgcctgaa gcagtacaga cagcaggtga gccacgactg cttcccctca
     1201 cctgaatcac actgtgccat taaatgaagg cattcaactc ccggttcccc cgaaaaaaga
     1261 cgttcaggta ccggagaaaa caatcacaag caaaaatccg gaagccgcag ctgcgcgaca
     1321 taatctggct actgttctga agcggaacgc cgatcggggc atgtttacca tcaacctgtc
     1381 atccggtcat gaacgaaccc tgttcgcatt tctcgatcct gcctgtccta actgcagact
     1441 tctggagccg gccttaaacg cctggccagc gacttcaatg tcgttatcta cccggtttca
     1501 gttattggtg gagaggaatc caccgaccgt gtggcaccac tgctgtgtga aaaagatgct
     1561 cagaaacgtg ctgccggctg gcaccgactg tattccgcag ataacggaat gatgacgcca
     1621 tcagaagaaa caacgcctgc agatgaaaca tgcctgaaag cagccagagc tgcgattgat
     1681 gttaacaatg tcgccttccg taagttcgga tttgccggta ccccatgggt gctgtccgac
     1741 accggctggc atctgcctac aggaattctg caggaaactg gcacgcttaa tttattcctg
     1801 aaaaccaccg acagcgagtc tggccatgag tgaacatcgc gttaaccccg agcttttaca
     1861 ccgtacagcc tggggaaatc ctgtctggaa tgcactccag agtctcaata tctatggctt
     1921 ttgcctggtg gcaagtcttg tggccagttt tatctggcca cttgcattac cggcatgtct
     1981 gttgttcaca ctgataacaa tgctggtatt tagtctgcag cgatggcgct gccctcttcg
     2041 tatgcccatg acgctggaat gtgctgaccc gtcacaggac agaatgatta aaagaagtct
     2101 tttcagcttc tggccaaccc tgttccagta tgaggtcatt ctggaatcac cggccagcgg
     2161 catattctat gttggttatc aacgcgtcag ggatattggc agggagttat ggctgagtat
     2221 ggacgatctg acccgccata taatgttttt cgccaccacc ggtggcggta aaacggaaac
     2281 catctttgcc tgggcgatta acccactttg ctgggcaaga ggattcaccc ttgtcgatgg
     2341 caaggcacag aatgatacgg caagaacgat ctggtatctg gccagacgct ttggacgtga
     2401 agacgatgtg gaagtcatta acttcatgaa cggaggaaaa tcccgcagtg aaatcattct
     2461 gagtggtgaa aaaacgcgtc ctcagtcaaa tacctggaat cccttctgtt acagcactga
     2521 ggcattcaca gcggagacaa tgcagtcaat gctgccccag aacgtccagg ggggcgagtg
     2581 gcagtcccgg gccattgcca tgaataaggc actggtattt ggtactaaat tctggtgtgt
     2641 gcgcgaaggc aaacaatgtc gctgcagatg ctccgtgaac atatgactct cgaagggatg
     2701 gccaaacttt actgtcgtgg cctcgatgat cagtggccgg aagaagctat cgcaccgtta
     2761 cgtaactacc ttcaggacgt tcccggtttc gacctgtcgc tggtcaggac accatcagcc
     2821 tggacggaag aacccagaaa acaacatgct tatctctccg ggcagttttc tgaaactttc
     2881 agcacattca cggaagcatt cgggatattt tgctgaagat tccggtgata tcgatatccg
     2941 tgacagcatt cacagcgaca ggattctgat ggtaatgatc cccgctctgg atacatcagc
     3001 gcatacaacg tctgctctgg gacgaatgtt tatcacccag aaagtatgat tctcgcccgt
     3061 gatttgggct accgcctcga aggaactgat tccgatgcac tggaagtcaa aaaatataag
     3121 gggcgtttcc cttatctgtg tttccttgat gaagtggggg catactatac ggaccgtatt
     3181 gctgtggaag ccactcaggt gcgttcactg gattttgctc tgattttgac ggcacaggac
     3241 caggaacgta tagaaggaca gacaacagcc acgaatactg caacactgat gcagaacacg
     3301 gggacaaaat ttgccggcag gattgtcagc gagggtagta cggccagaac gctgaaaagc
     3361 gccgccggtg aagaagccag ggcgagaatg aacaacctgc agcgtcagga cggcatattt
     3421 ggtgaatcat ggattgacag cccacaaatc agcatcctga tggaaagcaa gataaatgtt
     3481 caggagttga ttgaacttca tcctggcgag ttcttttcta ttttccgggg agaaaccgta
     3541 ccatcagcat cattcttcat ccctgacgat gagaaaagct gcagcagcga tcccgttgtt
     3601 ataaacaggt atatcagcgt ggatgcgcca cgcctggacc gtctccgccg tcttgttccc
     3661 cgtactactc aacgtcgaat accgtcaccg gaaaatgtca gtgccatcat tggcgtcctg
     3721 actgcaaaac cctccagaaa aagacgaaaa attcgcacag agccgcacac gatcgtggat
     3781 acattccagc aacgcattgc aggacgtcag gcggctatgg caatgctgga agagtacgac
     3841 acagatatca acgcacgtga aagtgccttg tgggaaacag cggtgaatac gcttaaaacg
     3901 accacacggg aagagcgacg aattcgctat atcacgctga acagaccaga actacctgaa
     3961 acaaaagagg aaaatcagat atcagtgaga gcagaaagag caggtataaa cctgttaacg
     4021 cttccacagg acaataacca cccgacaggg cgtcctgtaa acgggtttca tcataaaaaa
     4081 aacaaccgtc cggactggga cggaatgtac tgaacagtgg ccgcgccgga ttctggcgcg
     4141 gccttttttt taacctggag aaggcggttc ccagcgttta tcctgctccg gcggaggcag
     4201 tgatgcgtca tcgttgctgt tcgccgcgat ataatcgcgg tagtagcgtt cacttccttc
     4261 acggtgatga tccacgtccg gtcgcgtaat cctgtagtct tcgtgaccgg tacggcctgt
     4321 cacattatgc caggcaacca tttcagcaaa gacctggtta aattgtttgt cgtttccttc
     4381 tggttcaaaa cccatgcgtg gatcacgccc gaataatacc ggtgcaatca aatccagttt
     4441 atctgccagc gcattgcggt catggtgatt acggaaaaca ccctgtcacc ataatccgta
     4501 cagacaaact ctcccgtccg acggtcacgg aaacgaacac tcccgttctt ctgcagacgg
     4561 gcctcgagat caccggtatt accgtatacc ggcgttccgc ccggttcaca aagcacgaca
     4621 cagcggtctg tcgtcgttgt gcgtgacctg tctttacggc gatcgcgata atcccagcct
     4681 ctcaactgcg atacagcagc cctgtctccc tgagcagcct gaatttcgac ccactgacgg
     4741 taagagggtg gataccactt cccgtcagca ataagtttct gtcgctcatc cctgatgtct
     4801 tctttcagcc tgatcaatgc ctgcattcgc tgaacttctg caatgtgata atgcagctta
     4861 cgtaatgccg gatcatcgta ctgcgcacga atgtgtgact tccgcagacg gcatgcctga
     4921 tgaatttccc gacaacgttc ccataacgca gatctggttt acgccattgt tcacgccatg
     4981 ccagatagcg ggcacgcagg tcagcacggg cctcagctct ggcttcacgt cgctgccggc
     5041 gcagcattgg gtcacgttta ctgctgaccc cgtacctgtc actgacagcc agctccgggt
     5101 tatagcgact ttcaggttgc accctgtcaa acaaatctgc tggggcactc acaaaaggtc
     5161 cggctgtggc tctgcacgcc ccagtgtcag atcaggatga atgctgctgg ccttcaccgg
     5221 cgtttgctca tgattaaagg catcaacgac aacaagcccg tgatgttgtt tctgtagcat
     5281 cagtccctgt tgtgcaaaca gctcatgaca acgctgccag gtaaactcac cctgtgtgat
     5341 catttcttca atctgagggc tacaatggct gatgctgtat ccccacagac tttctttgtc
     5401 agaaaacagt tcgcgtttga tagccccctg cggccccgtg cctgtacgca tacggtgcca
     5461 ttctttatcg gtcgcaggac agctctccat acgacgacca cggaagcgtt ctgttggcgt
     5521 aacctggcgg aaaatatctg ttggtacagg ctgccagcca ccatcgagtt gattcacatt
     5581 gtcgagcgtc agcagcgacc atacgctgtc agcacgaacg ggagtctggt tatgatcata
     5641 accgtcacag ataaccaggt gaccatgctg aacacgcatc cataatcccg cttcagccag
     5701 tgtgcggtga atagcctggc agttttccag ccgtccttcc cgtgccattt ccggcagacg
     5761 ttctccaagg tgacgacagg catactgctg cagactttct gtttcagcga tttttccggg
     5821 cgaacatcag cggcaatgaa gtccggatta tagcgtcctg gtgcttccac ctggctgaaa
     5881 atgtcttttg gtactggcgt gtagtcaccc atttttttca tgagttttct gcacaccagg
     5941 agggaccaaa cgagtcaagc tgtacacctc ccctgttgcg atcccagcca tccatcacca
     6001 gaaattttcc gtccatctga gacaggtaca gaccatcttc cgcaagacga cgatgcaggg
     6061 ataaccagtc atgtacaggt tcactgcgta aaccagcgac cgctgtctgc gcaacatact
     6121 cgcgaaaagt ttgttttttt ccgcgtgtcc aggcgttctg gcgatcacgt tcaacggcag
     6181 ttttgcgaac gatacgattt cccggcgcat ggacccagca accgttatcc ggagcaaaac
     6241 catgcttcag ttcaagctca cggcaggcac ggctgagttt ttcctgactc catgaaaggc
     6301 aattgagata acctgtaacc ggatgcaccc ggttgaccgc aacgtgaaca tgcaggttat
     6361 cagtatcggt atgcacggca gagacatact gatgttcacc cagcccgaga gatttcaggg
     6421 tatggcgtac actgtcataa atctgttccg gacgcgggct ttcatgggcc tgccaggaaa
     6481 gaatgtagtg aaaaacagga tcagtattat ctttcgcata gtgtgcctgc tgtgcaatgt
     6541 actccatatc tgcagcggca gtttcaagcg atgtacagtt gtgaaaacag gtcacaccat
     6601 aaaagttaac ccattcgcag ccgtccttca tgacatcaac cagcgccacg aatgactcat
     6661 ttcgaagacg tgtcgcataa tcaacaagac gactgaagcg gtttcggtgc ggttgttcag
     6721 cctgcgaaga agaagataaa tccagttcct catcggtcat gtcatcccgg acagagacgt
     6781 aggacaccag gtcttcgaac gaagacttgc cgtcccttct ctttttcgga atgactgcat
     6841 tcaccggaga atcctcaacc ttccattaac tgtttacgca atgtattgta agttcggtta
     6901 tggcaacaag cacttctgcg tattccttgt caccggttct tttccctgaa caaacagatg
     6961 cttctgcatc cttcccagtc tcattaactc cttcagaaaa gcatcatctg tccgggagtt
     7021 aattcgctta ttcagtgctg catttcggat gtaagcagat acagtaagtc cggaatcttc
     7081 agcttttttc ctgatggttc atcctcaaca ggagagaatc tgagtgttct gacaactgtc
     7141 tttgccgtac ctcacttttc tttcttactg cactatcact cacttcaggc tccttacggg
     7201 gtgtcggggc gaagccctga ccagatggta attgtaatag cgtcgcgtgt gacggtatac
     7261 aattacacat cctgtcccgt ttttcaggcc atatagcctg ttattttgtg catcatagtc
     7321 ttatgcagac ggcagaggca gggcgcagcc ctgccaggag gcagtttttg atgataaaat
     7381 cagacttcaa catgaaacgg agagaaagtg ccaatggctt ctaaaaattt tactcagatg
     7441 atgacatcca gctggcaaaa gctgccttat cggaattgcc ggatctcacc gctcaacgga
     7501 aaacattgcg tgatttcttg atgccattcg tgatgacatt attgtcctgg taaggacaaa
     7561 gggttataca ctggcagatg ttcgtgacac gctcaaaaat gcagggtatg aagtaggaga
     7621 gaaggcactt cgtgacatat tcgggaagca gaatcgaaaa aaccatcccg ccgttcttcc
     7681 tctaaaacag cacctaaaaa gacagattca ggcaggaaag atgcatcgat atgaacaaca
     7741 attgatgtga caatgtcaca tcgtgttctg agattatgat gattacagaa aaaaaaagcc
     7801 cggcataaat accagggctt tctgtttaat cagactcgtg gcggcaatgc accaggataa
     7861 tgccgtgcaa taatatcatt cagtttttca acaaggtcag aacgcttaaa gacaatgtgt
     7921 acggtccctt tctggtagcc acgcaccgta aatattcccc ttctaacagc ttaccgacat
     7981 tgtcgccgtt cccgagaaat tcaccatact gcgccccgat gctgttcctg aagtcaggaa
     8041 cgttgcgccc ctccagcaga taaaacggac gtaccaggtc atccagtgct cccgtccgct
     8101 gcagtctagc gtcacacgcc cgttactcca gcggtacagc aggtttcaat aatgattttc
     8161 ttacccaggc ggcagggatt attggttctg taatccatga caatttccgg tagacatcaa
     8221 tcagcccctg ttcaaacgtc tgcatcttgc tggcattaag atggcggaat gttgccagta
     8281 cattgtccag ggtgatttca ggacaggtgt cgctcatcag ctggctgttc cactcatcac
     8341 gctggcatga actcatgaag gtgtacatgc ccgtatctgt catcaggcgg tcccagattt
     8401 ccggtcggca agacgggtga tatccttttc gcttcttccg gtttatccat gcgacgaacg
     8461 gaatcagtca cgcagtcttc aaacccgtaa agaaaaaata tcacctgacg catcgagcag
     8521 aatcctgcgc gctgtcgcca ggcattccat cgcctgcatg aacagtgcaa taccttcgtt
     8581 acgctgcgcg atgatgcggt cgatggccac agacggaata acatctgtcg tgccggcagt
     8641 attcaggtca gcagtgggta agtctgattc atcacattgt ctcctcatcg tcacgactgc
     8701 cgggtcagcc aggtcatttc ccggaggagc gaagctcctt ctgccgtccg ggtctcgggg
     8761 atttccccgc agtggacctt tccgacgggg acgctcacca cgtcacgggg cagttgtccg
     8821 ggggcagacg caaaagtttt gacgcctgcg gcaaaaagtt ttgtggctgg tacatctctc
     8881 ccctggatga ctgaaacggg atgtggtcgt gtatgtaccc ccggaaagtc cgtctgcgga
     8941 gaacaggccg cagtgcggcg gcagacaggc agagacagct gcgccatcag gcgcagtggc
     9001 cttacacacc gtaaggtgtg cagggagcgc cgcatcccgg cgcgactgaa gaaaaacgac
     9061 ggcacaacaa tggcatgtgg tggtcactgg cacaccactc ccgcacaggt gaaacagaga
     9121 aaccgcaacg acattacaac caaaaaaagt gaaatttctt cacttttttt cacttattta
     9181 tcgcgctgat tggcaatgtg atgataaaat aaagtgaagt tttttcactt ttcaaaggtg
     9241 ttttatgact ggctgggaac tgcgaatctg gcgaaaaagc atgctctggt cacgcgaaaa
     9301 agcggccaga gaatttggcg tgacgcagag aacatggcac gcctgggaaa atgcggaaca
     9361 ggtggaacgt caccgtctgg aggaccaccc aggcactttc agtccgtgac ctgctgcctc
     9421 acatgcaggg gatgcgcaag gcagatatca tcaggcgtct ggaaatgaac tggggaaacg
     9481 gcagaggatg tgtgagcgtg attcacagcc tggcagaatg ccaggctacg ctcatcagaa
     9541 acagtttttc agttcttcac cggaaagtcc ggctgcctcc tgaatccgtt ccggcgtcca
     9601 gcccatatcc cgcagccggc aggctgcttc ccgcgccact tcaagcgcac cttcttaaaa
     9661 ccttcttaaa accttcgtcg aaacctcgtc gaaacctttc tgtataagac gttctgcaat
     9721 cgtcatactg ttttctctgc tcctgctggc aaaggcccgg tattttgccg ggctgtgagg
     9781 ttctcatcag tgaatgattt cttcagttcg tcatcggaaa gtccggtagc ctgcttaatc
     9841 tgctccggcg tcatccccat tttctgagct ggcacgcaat agccagggct tttcaagcgc
     9901 tccttccatc gtccttccat cctgccttcc tggcgacctt tttgctcaag agtctgcgat
     9961 agtcatcagt ttatcctcat gctgcggcag acgctcagcc agcgcccgca taaattccgg
    10021 tgatacagag tcccctgcgt tacgatgtaa taatcgaaca cgcacctgct cgtctgaaat
    10081 ttcaccatga cctgcggggt tgtccacagc tcatcatgtc acgaggcgaa tgcttatcac
    10141 agcgtcagtg cggcatccgg cgaggttcaa tgattcattg tcatcaacga cagtgaatca
    10201 accagcggaa acggttgttt atatagccga gcccgattct tacatgtttc cgcatgttac
    10261 ttacagtgtg gccatgagaa catctggagc acacaaaacg ttgcactggt gattccgggc
    10321 tgttctatca tggtgcgcag ccgtatccgt acagcatgaa ctggctggac gttttgagat
    10381 ccggcgcttg cggctaaaat atacacaaag ccgtttccgc tggttgatat cactgtcgtt
    10441 gatgacaatg aaatcatgaa ccatcgccgg atgccgcact gacgctgctg atgaagcata
    10501 tccgccatcg tgactgatgg agctgctgac actcccgcag tcatggtgga aatttcagac
    10561 gagcaggtgc gtgtttgatt cattacatcg taacgcaggg actctgtatc accggaattt
    10621 tgcgggcgct ggtgagcgtc tgccgcagca gggataaact gatgactatc gcagaacgtc
    10681 ttgagcaaaa aggtcgccag gaaggcagga tggaaggacg catggaagga gcgcttgaaa
    10741 aagccctggc tatgcgtgcc agcttcagaa aatggggatg acgccggagc agattaagca
    10801 gctaccggac tttccgatga cgaactgaag aaaatcattc actgatgaga acctcacagc
    10861 ccggcaaaat accgggcctt ttgccagcag gagcagagaa aacagtatga cgattgcaga
    10921 acgtcttata cagaaaggtt tcgacgaagg tttcgacgaa ggttttaaag aaggttttaa
    10981 gaaaggtgcg cttgaagtgc gcgggaagca gcctgcggct gcgggatatg gctggacgcc
    11041 ggaacggatt caggaggcag ccggactttc cggtgaagaa ctgaaaaaac tgtttcctga
    11101 tgagcagtag cctggcattc tgccaggctg tgaatcacgc tcacacatcc tctgccgttt
    11161 cccccagttc attttccaga cgcctgatga tatctgcctt gcgcatcccc tgcatgtgag
    11221 gcagcaggtc acggactgaa agtgcctggg tgtcctccag acggtgacgt ccacctgttc
    11281 cgcattttcc caggcgtgcc atgttctctg cgtcacgcca aattctctgg ccgcttttcg
    11341 cgtgaccaga gcatgctttt tcgccagatt cgcagttccc agccagtcat aaaacacctt
    11401 tgaaaaagtg aaaaaacttc actttattta tcatcacaat tgccaatcag cgcgataaat
    11461 aagtgaaaaa aagtgaagaa atttcacttt ttttggttgt aaatgtcgtg cggttttctc
    11521 tgttttcacc tgtgcgggag tggtgtgcca gtgaccacca catgccattg ttgtgccgtc
    11581 gtttttcttc agtcgcgccg gatgcggcgc tccctgcaca ccttacggtg tgtaaggcca
    11641 ctgcgcctga tggcgcagct gtctctgcct gtctgccgcc gcactgcggc ctgttctccg
    11701 cagacggact ttccgggggt acatacacga ccacatcccg tttcagtcat ccaggggaga
    11761 gatgtaccag ccacaaaact ttttgccgca ggcgtcaaaa cttttgcgtc tgcccccgga
    11821 caactgcccc gtgacgtggt gagcgtcccc cgtcggaaag gtccactgcg gggaaatccc
    11881 cgaagacccg gacggcagaa ggagcttcgc tcctccggga aatgacctgg ctgacccggc
    11941 agtcgtgacg atgaggagac aatgtgatga atcagacttt acccactgct gacctgaata
    12001 ctgccggcac gacagatgtt attccgtctg tggccatcga ccgcatcatc gcgcagcgta
    12061 acgaagtatt gcactgttca tgcaggcgat ggaatgcctg gcgacagcgc gcaggattct
    12121 gctcgatgcg tcaggtgata ttttctttac gggtttgaag actgcgtgac tgattccgtt
    12181 cgtcgcatgg ataaaccgga agaagcgaaa aggaatatca cccgtcttgc cgaccggaaa
    12241 atctgggacc gcctgatgac agatacgggc atgtacacct tcatgagttc atgccagcgt
    12301 gatgagtgga acagccagct gatgagcgac acctgtcctg aaatcaccct ggacaatgta
    12361 ctggcaacat tccgccatct aatgccagca agatcagacg tt
//
