LOCUS       NZ_CP027259             6812 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain EC11 plasmid unnamed4, complete sequence.
ACCESSION   NZ_CP027259
VERSION     NZ_CP027259.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08619547
            Assembly: GCF_002996665.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Bai,F.
  TITLE     Whole Genome Sequence of Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6812)
  AUTHORS   Bai,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2018) College of Food Science and Technology,
            Shanghai ocean university, Ministry of Agriculture, 999# Hu Cheng
            Huan Road, Shanghai 201306, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-NOV-2017
            Assembly Method :: A5-miseq v. v20150522; CANU v. v1.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 358.0x
            Sequencing Technology             :: Illumina MiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002996665.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 03:27:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,035
            CDSs (total)                      :: 4,918
            Genes (coding)                    :: 4,742
            CDSs (with protein)               :: 4,742
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 80 of 176
            Pseudo Genes (incomplete)         :: 100 of 176
            Pseudo Genes (internal stop)      :: 47 of 176
            Pseudo Genes (multiple problems)  :: 45 of 176
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP027259.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC11"
                     /isolation_source="cow dung"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="China:Shanghai"
                     /collection_date="2016-05-10"
                     /collected_by="Fengjia Bai"
     gene            complement(join(6705..6812,1..412))
                     /locus_tag="C6C13_RS27695"
                     /pseudo=""
     CDS             complement(join(6705..6812,1..412))
                     /locus_tag="C6C13_RS27695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /name="MbeB family mobilization protein"
     gene            complement(<450..1148)
                     /locus_tag="C6C13_RS27700"
                     /pseudo=""
     CDS             complement(<450..1148)
                     /locus_tag="C6C13_RS27700"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1090..1413)
                     /locus_tag="C6C13_RS26575"
                     /old_locus_tag="C6C13_26570"
     CDS             complement(1090..1413)
                     /locus_tag="C6C13_RS26575"
                     /old_locus_tag="C6C13_26570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1477..1668
                     /locus_tag="C6C13_RS26580"
                     /old_locus_tag="C6C13_26575"
     CDS             1477..1668
                     /locus_tag="C6C13_RS26580"
                     /old_locus_tag="C6C13_26575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2338..2499
                     /locus_tag="C6C13_RS27705"
     CDS             2338..2499
                     /locus_tag="C6C13_RS27705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596883.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRARFI
                     QMLTEFLK"
                     /name="replication initiation protein"
     gene            complement(2688..2825)
                     /locus_tag="C6C13_RS26600"
                     /old_locus_tag="C6C13_26595"
     CDS             complement(2688..2825)
                     /locus_tag="C6C13_RS26600"
                     /old_locus_tag="C6C13_26595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329514.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_024139133.1"
                     /translation="MCGKILLILFFIMTLSACQVNHIRDVKGGTVAPSSSSRLTGVEIK
                     "
                     /name="colicin release lysis protein"
     gene            2853..3377
                     /gene="cni"
                     /locus_tag="C6C13_RS26605"
                     /old_locus_tag="C6C13_26600"
     CDS             2853..3377
                     /gene="cni"
                     /locus_tag="C6C13_RS26605"
                     /old_locus_tag="C6C13_26600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_061352107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin N immunity protein"
                     /protein_id="WP_047649571.1"
                     /translation="MDIKDRNKISKKISFSLLLLLSPFALIFFSYNNAPIPLLEKIIAY
                     LSLPGFHSLNNPPLSEAFNLYVHTAPLAAISLFIFTHKELELKPKSSPLRALKILTPFT
                     ILYISMIYCFLLTDTELTLSSKTFVLMSKNDLFLSFFYITLYIGIYIFTYLYFWFLIGT
                     YKLFTRGGILP"
                     /name="cni"
     gene            complement(3386..4549)
                     /gene="cna"
                     /locus_tag="C6C13_RS26610"
                     /old_locus_tag="C6C13_26605"
     CDS             complement(3386..4549)
                     /gene="cna"
                     /locus_tag="C6C13_RS26610"
                     /old_locus_tag="C6C13_26605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin colicin N"
                     /protein_id="WP_032488335.1"
                     /translation="MGSNGADNAHNNAFGGGKNPGIGNTSGAGSNGSASSNRGNSNGWS
                     WSNKPHKNDGFHSDGSYHITFHGDNNSKPKPGGNSGNRGNNGDGASAKVGEITITPDNS
                     KPGRYISSNPEYSLLAKLIDAESIKGTEVYTFHTRKGQYVKVTVPDSNIDKMRVDYVNW
                     KGPKYNNKLVKRFVSQFLLFRKEEKEKNEKEALLKASELVSGMGDKLGEYLGVKYKNVA
                     KEVANDIKNFHGRNIRSYNEAMASLNKVLANPKMKVNKSDKDAIVNAWKQVNAKDMANK
                     IGNLGKAFKVADLAIKVEKIREKSIEGYNTGNWGPLLLEVESWIIGGVVAGVAISLFGA
                     VLSFLPISGLAVTALGVIGIMTISYLSSFIDANRVSNINNIISSVIR"
                     /name="cna"
     gene            4808..5245
                     /locus_tag="C6C13_RS26615"
                     /old_locus_tag="C6C13_26610"
     CDS             4808..5245
                     /locus_tag="C6C13_RS26615"
                     /old_locus_tag="C6C13_26610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024197979.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGFVSYRTSQDGRR
                     EFETSELIRAYGELKQNETPERHSAGQAENPHDQPTERILRELNELKQCLTLMLEDKQA
                     QDMDRKRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            5326..5745
                     /locus_tag="C6C13_RS26620"
                     /old_locus_tag="C6C13_26615"
     CDS             5326..5745
                     /locus_tag="C6C13_RS26620"
                     /old_locus_tag="C6C13_26615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_024168414.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQHFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            5939..6109
                     /locus_tag="C6C13_RS27095"
     CDS             5939..6109
                     /locus_tag="C6C13_RS27095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005074609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_163448562.1"
                     /translation="MSKAPIGTTASTGQKCPESGVWKVVGTPSTTAPIAKGNVMPPYNG
                     KAVTWKLTQYA"
                     /name="hypothetical protein"
     gene            complement(6465..6698)
                     /locus_tag="C6C13_RS26885"
     CDS             complement(6465..6698)
                     /locus_tag="C6C13_RS26885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_097314068.1"
                     /translation="MTELEIHLLNALEQQQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1754..1841)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          1855..2410
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.086"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(4..1100)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.448"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(6362..6810)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.904"
                     /coverage="99.0"
                     /evalue="2.06e-61"
ORIGIN
        1 cgccccatgt cttcgcgttc agtttctgca atgtctcttc ctgcttcctg atttcttcca
       61 ggttcgtctg tatccgtccg ccaagatacc agagcgtccc gccactcgcg gtaaacagga
      121 gaaagactat ccccagtaac atcatgcccg tattccctgc cagctttaac acgtccttcc
      181 tgtgctgcat catcgcctct ttcacccctt cccggtgttt ttccagcgat ttctctgtcg
      241 aggctgtgaa cagggctata gcgtctctga ttttcgtctc gtttgaggtc acagcctcgc
      301 tgacagactc gccgagcttc ctgaactcgt cgttcagcat tttctctgta gattcggctc
      361 tctctttcag ctttttctcg aactccgcgc ccgtctgtaa aagattgctc ataaaacgct
      421 cctttcagtc tgatattcct cccgccgttc gggtctgcaa tgctgatact gcttcgcgtc
      481 accctgacca cttcaagccc cgcctctgtg agcgcctgaa tcacatcctg acggcctttt
      541 atctctccgg catggtaaag cgcgtctatc ccgcgtgtaa cgccctcagc aatcgcctgt
      601 ttcgtttccg gcagattatc agagagtgtc agcgtcctgc ggttctccgg ggcgttcggg
      661 tcatgcagcc cgtaatggtg attaaccagc gtctgccagg catcaattct aggcctgtct
      721 gcgcggtcgt agtacggctg gaggcgtttt ccggtctgta gctccatgtt cggaatgaca
      781 aaattcagct caagccgtcc cttgtcctgg tgctccaccc acaggatgct gtactgattt
      841 ttttcgagac cgggcatcag tacacgctca aagctcgcca tcactttttc acgttctccc
      901 ggcggcagct ccttctccgc gaacgacaga acaccggacg tgtatttctt cgcaaatggc
      961 gtggcatcga tgagttcccg gacttcttcc ggattaccct gaagcaccgt tgcgccttcg
     1021 cggttacgct ccctccccag caggtaatca accggaccac tgccaccacc ttttcccctg
     1081 gcatgaaatt taactatcat cccgcgcccc ctgttccctg acggccagac gcagccggcg
     1141 cagctcatcc ccgatggcca tcagtgcggc caccacctga acccggtcac cggaagacca
     1201 ctgcccgctg ttcaccttcc tcgctgtctg attcaggtta ttcccgatgg cggccagctg
     1261 acgcagtaac ggcggtgcca gtgtcggcag cttcccggaa cgggcaaccg gctcacccag
     1321 gcagacccgc cgcatccata ctgccagttg tttaccctca cagcgttcaa gtaacctggc
     1381 atgttcatca tcagtaaccc gtatcgtgag catcctctcg cgtttcatcg gtatcattac
     1441 cccatgaaca gaaatccccc ttacacggag gcatcagtga ctaaacagga aaaaaccgcc
     1501 cttaacatgg cgcgctttat cagaagccag acattaacgc tgctggaaaa gctcaacgaa
     1561 ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc cgatgagctt
     1621 taccgcagct gcctcgcacg tttcggggat gacggtgaaa acctctgaca catgcagctc
     1681 ccggagacgg tcacagcttg tctgtgagcg gatgccggga gcagacaagc ccgtcagggc
     1741 gcgtcagcag gttttagcgg gtgtcggggc gcagccctga cccagtcacg tagcgatagc
     1801 ggagtgtata ctggcttaac tatgcggcat cagtgcggat tgtatgaaaa gcacgccatg
     1861 ccgggtgtga aatgccgcac agatgcgtaa ggagaacata ccgcatcagg cgctcttccg
     1921 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1981 actcaaagac ggtaatacgg ttatccacag aatcagggga taaggccgga aagaacatgt
     2041 gagcaaaaga ccaggaaccg gaaaaaggcc gcgccgatgg cgtttttcca taggctccgc
     2101 ccccctgacg agcatcacaa aaaccgacac tcaagtcaga ggtggcgaaa cccgacagga
     2161 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2221 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2281 agctcacgct gttggtatct cggcttggtg taggtcgttc gctccaagct gggctgtgtg
     2341 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2401 aacccggtaa gacacgcctt aacgccactg gcagcagcca ctggtaaccg gattagcaga
     2461 gcgagattta tccagatgct tacagagttc ttgaagtgat ggcccaactg cggctacact
     2521 gaaaggacag tatttggtat ctgcgctctg ctgaagccag ttacctcggt taagcagtcc
     2581 ccaactgact taaccttcga ctaaaccgcc tccccaggcg gttttttcgt ttacaggcag
     2641 cagattacgc gtagaaaaaa aggatctcaa gaggatcctt tgatctttta cttaatttca
     2701 acccctgtta gtctggatga tgatgatgga gcaacagtcc ctcctttcac atcacggatg
     2761 tgattgacct gacatgcact aagtgtcatg ataaaaaaca agattaataa aattttccca
     2821 cacataattt tcaaccatcc aaagaaagca acatggatat aaaagacaga aataagatat
     2881 caaaaaaaat atcattcagt cttctgctct tactttcccc attcgcatta atatttttca
     2941 gttataataa tgcaccaata ccactcctcg aaaaaatcat cgcataccta tccctaccag
     3001 gatttcattc attaaacaac ccgcccctaa gcgaagcatt caatctctat gttcatacag
     3061 cccctttagc tgcaatcagc ttattcatat tcacacacaa agaattagag ttaaaaccaa
     3121 agtcgtcacc tctgcgggca ctaaagatat taactccttt cactattctt tatatatcca
     3181 tgatatactg tttcttgcta actgacacag aactaacctt gtcatcaaaa acatttgtat
     3241 taatgtcaaa aaacgatctg tttttgtctt ttttctatat aacactatat attgggatat
     3301 atatattcac atatttgtac ttttggttcc ttataggaac atataagcta tttaccaggg
     3361 gaggaatact cccctgacac taatatcatc gaataacact agatataatg ttatttatat
     3421 tcgaaactcg atttgcatct atgaaagatg acaagtaact aatcgtcatt attcctatta
     3481 cccccaacgc agtaactgca agtccagaga ttgggagaaa actcaacaca gccccgaata
     3541 aactaatagc aactccagca acaacgccac caatgatcca tgattcaacc tccaacagga
     3601 gaggtcccca gttgccagta ttgtatccct caatgctttt ttccctaatt ttctcaacct
     3661 ttatagctaa atcagcaacc ttaaatgcct tgccaagatt accaatctta ttagccatgt
     3721 ccttagcatt aacctgtttc caggcattca caatggcatc cttatcagat ttgtttactt
     3781 tcatctttgg atttgctaac actttattaa gtgaagccat tgcttcatta tagctacgaa
     3841 tattacgacc atggaagttt ttaatatcat tggcaacttc cttagctaca tttttatatt
     3901 ttactccaag atactcgcca agcttatccc ccataccaga cacaagttca ctagccttta
     3961 gcaaggcttc tttttcattt ttttcttttt cttccttcct aaataataaa aactggctca
     4021 caaacctctt cacaagttta ttgttatatt tcggtccctt ccagttcaca taatcaactc
     4081 tcattttatc aatattacta tctggaacag taaccttaac atactgacct tttctggtgt
     4141 gaaaagtata tacctctgta cctttaattg attccgcatc aattaatttt gccaacaatg
     4201 aatattcagg atttgacgaa atataacgac ctggtttcga gttgtcaggt gtgattgtta
     4261 tctctccaac cttagcactc gctccatcac cgttattacc tcgatttcca ctattccctc
     4321 caggtttagg ctttgaatta ttgtccccat gaaatgtaat atggtaagaa ccatcactgt
     4381 ggaagccatc atttttatga ggcttattac tccatgacca tccattggaa tttcctcggt
     4441 tacttgatgc actaccatta cttcctgcgc cactggtatt accaatgccc ggatttttcc
     4501 ctccaccaaa agcattgtta tgtgcattat ctgcgccatt actacccata ataaatccct
     4561 ctttgacttt taaaacaaac cgttaaaaat aaatactgta cacataacca ctggttttat
     4621 atacagttta aaattacgcc gctgcgtttt tcctgtcaac cctgggaatt ttcgtttttg
     4681 tgaaaacgat caaaaaaaca gccacccaca ccacgtgcta ttcccgccgg ataccacaaa
     4741 aaccagcaca aacattaccg ttctcagacc tcattatgtt ttactgaaac catgagatga
     4801 gacacctatg ggacactgtc actttatggc atggcacaca ctccgtgacg cactgaaaat
     4861 gacaggcaga tcgcgctcac agttttaccg tgatatgcgc gcaggctttg tcagttaccg
     4921 taccagtcaa gacggacgac gggagtttga aaccagtgaa ctgatccggg catacggcga
     4981 attaaagcag aatgagacac cagaaaggca cagtgcggga caggcagaaa atccacatga
     5041 tcagccgaca gaacgcattc tccgggagct gaatgagctg aaacaatgcc tgacgctgat
     5101 gcttgaggac aaacaggcac aggatatgga tcgcaaacgc caggaagcag aacgggaaca
     5161 gctgcagaat gagatagccc agctcaggca ggcactggaa ctggaaaaga aacggggatt
     5221 ctggtccagg ctgttcggtc gctgaacgct gtcagagact gatgataaaa tagtcttcgg
     5281 ataataactc accgagaata aacacttgaa ggtagggaga cactcatgag acgtaccgga
     5341 aacaaactgt gtcttatcgc catgataaca gcaacagtag ctctcacagc ctgtacccca
     5401 aagggcagcg tggaacaaca tacccggcat tacgtatatg cttctgatga cggttttgat
     5461 cctaactttt ccacccagaa ggccgacaca acacgaatga tggtgccttt ttttcggcag
     5521 ttctgggata tgggagctaa agataaagcg acaggaaaat cacggagtga tgtgcagcaa
     5581 cgcattcagc actttcacag tcaggaattt ttaaactcac tccgaggcac aacacaattt
     5641 gcgggtaccg attaccgcag caaagacctc accccgaaaa aatccaggct gctggctgac
     5701 accatttcgg ctgtttatct cgatggctac gagggcaggc agtaagttga ttttccataa
     5761 gtcctttata acaaaaccaa aacttacaac gccaaaatca tatacaactt tgttaaaaat
     5821 atgaccacaa tcacatctgt gaaaaaacga cctttaagcc cgcttgaaag ctgatttcta
     5881 aaccagaaat aagtattgct acacaagcat acggataaaa catacaagag gaatcaatat
     5941 gtctaaagcc ccaattggta caacagcttc aaccggacaa aaatgccctg aaagcggtgt
     6001 ttggaaagtg gtaggaacgc cttcaactac agcaccaatt gccaaaggca atgtaatgcc
     6061 tccgtataac ggaaaagccg taacttggaa attaacccaa tacgcttgat atctcaaaag
     6121 cccccaccgg gggctttttc gttattcttg gatttaccat aatcccttaa ttgtacgcac
     6181 cgccaaaacg cgttcggtgc gatcacggca gcagacaggt aaaaatggca acaaaccacc
     6241 cgaaaaactg ccgcgatcgc gcctgataaa ttttaaccgc atgaatacct atgcaaccag
     6301 atggtacagg ccacattaac ccctcttaat ccactgaagc tgccattttt catggtttca
     6361 ccatcccagc gaaggaccat ccagcgtgcg ttcctgtatt tccggctgac gctcccgttc
     6421 cagggataac gcatgttcgc gctcctgtat cagccgttcc tctcttatct ccagttctcg
     6481 ctgtataact ggctcaagcg ttctgtctgc tcgctcaagt gctgcacctg ctgactcaac
     6541 tgcatgaccc gctcgttcag catcgcgttg tcccgttgcg taagcgaaaa catcttctgc
     6601 aattccacga aggcgctctc ccattcgctc agccgctgca tatagtcctg ttgctgctgc
     6661 tctaaggcgt tcagcaaatg tatttccagc tctgtcactc tgtgtcactc cttcagatgt
     6721 acccactctt tcccctgaaa gggaatcacc tccgctgatt tcccgtacgg aaggacaagg
     6781 aatttcctgt tcccgtcctg cacaaactcc ac
//
