LOCUS       NZ_CP027201             2088 bp    DNA     circular CON 17-JUN-2024
DEFINITION  Escherichia coli strain WCHEC025943 plasmid p3_025943, complete
            sequence.
ACCESSION   NZ_CP027201
VERSION     NZ_CP027201.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07510673
            Assembly: GCF_002811555.3
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain WCHEC025943
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 2088)
  AUTHORS   Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   4  (bases 1 to 2088)
  AUTHORS   Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002811555.3-RS_2024_06_17
            Annotation Date                   :: 06/17/2024 07:46:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,298
            CDSs (total)                      :: 5,180
            Genes (coding)                    :: 4,917
            CDSs (with protein)               :: 4,917
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 83 of 263
            Pseudo Genes (incomplete)         :: 171 of 263
            Pseudo Genes (internal stop)      :: 54 of 263
            Pseudo Genes (multiple problems)  :: 39 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP027201.2.
            On Oct 9, 2018 this sequence version replaced NZ_CP027201.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WCHEC025943"
                     /isolation_source="Sewage"
                     /db_xref="taxon:562"
                     /plasmid="p3_025943"
                     /geo_loc_name="China: Sichuan, Chengdu"
                     /collection_date="2017-04"
     gene            join(1809..2088,1..686)
                     /locus_tag="CXB56_RS01115"
                     /old_locus_tag="CXB56_01115"
     CDS             join(1809..2088,1..686)
                     /locus_tag="CXB56_RS01115"
                     /old_locus_tag="CXB56_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_100728248.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNTAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLAIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            690..956
                     /locus_tag="CXB56_RS01120"
                     /old_locus_tag="CXB56_01125"
     CDS             690..956
                     /locus_tag="CXB56_RS01120"
                     /old_locus_tag="CXB56_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          115..346
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.571"
                     /coverage="100.0"
                     /evalue="9.53e-117"
ORIGIN
        1 tttatcaggc tctaccgaaa atcgttgtgg attacccgtc ttcccgatgg ttgtttctga
       61 cgttaactgt caggaactgc gagataggtg aacttggaac agtccttaca gcaatgaata
      121 cggcgtttaa gcgaatggaa aagcgaaagg agctatcacc tgttcagggg tggatcaggg
      181 ctacggaggt gacgcgaggt aaggatggca gcgcacatcc gcattttcac tgtctgctga
      241 tggtgcaacc ttcttggttt aaagggaaga actacgttaa gcacgaacgt tgggtagaac
      301 tctggcgcga ttgcttgcgg gtgaactatg agccgaatat cgatattcgg gcagtaaaaa
      361 ctaagacagg tgaggttgtg gccaacgttg ccgagcaact gcaaagcgcg gttgctgaaa
      421 cgctgaaata ctccgttaaa ccggaagata tggcaaacga tcctgagtgg tttcttgagc
      481 tgacgcggca gcttcacaag cgccgtttta tctcgaccgg tggagcgcta aaaaacgtcc
      541 tccagttgga tcgagaaacc aatgaggatc ttgccattgc cgacgatgta ggggatggca
      601 ctgatgacgg gaagcggacg gcgtttgtct gggattcagg taaacggcgt tacaaacgcg
      661 cccctgagaa ggataaatcg gattaacgta tgaatattaa tattgaatac ctgaatggaa
      721 ataagactat tggtttattt tttttaagaa gtgaagcggt gattcctgac aggtttaaaa
      781 accttatttt gcttattgat ggattaagtt ttggcacatt tggttttcat ccgcacgaag
      841 gttttgagga tgaattaatt ttatatattc agaaaacaaa cgagagggta aaaactcttt
      901 ttgtgaaaat agatttaaac ggtatcaagt ttggattttt aagaacgcat tcttagttct
      961 ggaaaagagc cagcggcagg ctgaggtgat aggtacgaga ttgcatgcaa tctctagtgc
     1021 tctgtctatc ctgcattatc ctcagcatta tcctcagcct tgccaactcg acaccaatgc
     1081 aggatagaca atccgatgtc aaatgttaac actctgcgag tggtacattt tccccggatt
     1141 atcgtcctga gcctgccgct ggctctcttt ctaccgcctc gctttgctcg ttgctcaacg
     1201 cctcacagac acggattaaa atccgcatcc gttcaccgtt ttttaaagtc cgttaaaagc
     1261 atgatgccat ctccgagagt taatctcgtc aaatgctaaa tcgtgggggt cccctttggg
     1321 gttccgattt agtgattgac gacaccaccg attaaaaaac ttatgcgggg tggatggttt
     1381 cacgaagtga ggccatccac ctgtaagaca gggttttgtt tttatcccct gttttggtga
     1441 tcgggtgtgt ggaaaaggtt ggggtaagcc gttcggggtg cttgttttgg ggggttaaaa
     1501 ttgtggttat tttttgcgca attctcgcgc gtgatccttg tatttatact taagggataa
     1561 atggcgggta tgaaatagtg gtttagccca gtaatgacga ggctttgagt gggttttgac
     1621 aggtcaaaga aaatggagca gaattgaggc gtttttaatc ggcgttgggg agtgcgtcaa
     1681 cactccccaa catttcgaat gtgtcacctc agcggcaaac tctggtgaca tgtactggct
     1741 cgcaatgcac aggtacgtga tgaatatacc acatcaaatc acagcctgcc cagatcggag
     1801 caggcttaat gtcagaagat aaattccttt cggactacag cccccgtgat gcagtttggg
     1861 atacccagcg cacgcttacc gattctgtcg ggggtatcta ccagactgct gctgaattcg
     1921 agcgctatgc actccgtatg gcctcctgta gcggtttgtt acgttttggt tggtctacca
     1981 tcatggaaac cggagaaacg cgcctacggc ttcgtagtgc gcaattttgc cgtgtccgtc
     2041 attgccctgt ctgccagtgg agaagaaccc tcatgtggca agcccgtt
//
