LOCUS       NZ_CP027187             6078 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter baumannii strain AR_0052 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP027187
VERSION     NZ_CP027187.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04014893
            Assembly: GCF_003547115.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2018) Bioinformatics, Institute for Genome
            Sciences, University of Maryland, Baltimore, 801 West Baltimore
            Street, room no 646, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.6.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 14.08x
            Sequencing Technology             :: PacBio;Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 21:37:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,222
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 59 of 148
            Pseudo Genes (incomplete)         :: 86 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 28 of 148
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP027187.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="AR_0052"
                     /db_xref="taxon:470"
                     /plasmid="unnamed3"
     gene            complement(143..676)
                     /gene="aadB"
                     /locus_tag="CSB68_RS21250"
                     /old_locus_tag="CSB68_0001"
     CDS             complement(143..676)
                     /gene="aadB"
                     /locus_tag="CSB68_RS21250"
                     /old_locus_tag="CSB68_0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(767..1453)
                     /locus_tag="CSB68_RS21255"
                     /old_locus_tag="CSB68_0002"
     CDS             complement(767..1453)
                     /locus_tag="CSB68_RS21255"
                     /old_locus_tag="CSB68_0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     gene            2456..2863
                     /locus_tag="CSB68_RS21260"
                     /old_locus_tag="CSB68_0003"
     CDS             2456..2863
                     /locus_tag="CSB68_RS21260"
                     /old_locus_tag="CSB68_0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2853..4667
                     /locus_tag="CSB68_RS21265"
                     /old_locus_tag="CSB68_0004"
     CDS             2853..4667
                     /locus_tag="CSB68_RS21265"
                     /old_locus_tag="CSB68_0004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4776..5261
                     /locus_tag="CSB68_RS21270"
                     /old_locus_tag="CSB68_0005"
     CDS             4776..5261
                     /locus_tag="CSB68_RS21270"
                     /old_locus_tag="CSB68_0005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     gene            5394..6062
                     /locus_tag="CSB68_RS21275"
                     /old_locus_tag="CSB68_0006"
     CDS             5394..6062
                     /locus_tag="CSB68_RS21275"
                     /old_locus_tag="CSB68_0006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     ARG             complement(147..676)
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             complement(144..688)
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          2854..4664
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caaacaatcg aaaggtcaga ctattttgca aatgttgaat taacaaggtt agtagtaaag
       61 cagtaaatcc gataacggta gcagtgtttc ctaacacctg agttaagccg cgccgcaaag
      121 cggcgtcggc ttggacgaat tgttaggccg catatcgcga cctgaaagcg gcacgcaaga
      181 cctcaacctt ttccgccccg agtgaggtgc atgcgaacct gtaggactct atgtgctttg
      241 taggccagtc cactggtggt acttcatcgg catagtaaaa gtaatcccag atgatcgcct
      301 cccagctgtt acaacggact ggccgcccgg cgatgacacc ctcagccgcc tctgggcacg
      361 agccctgcgg agcctccgcg atttcatacg cttcgtctgc ccaccaagca ggttcgcagt
      421 caagtaactc atccccgatc tccgctaaga atccatagtc caactcctcc gtgacgcgcc
      481 cgccgagcat ttcaactatt gcctcgagct cgccgcgcct ctcgccggga aaagtcagat
      541 caatatcatc gtgcttgcgt gttacacgcc ctagccgtgc atcgatcgcc cagcccccac
      601 cgatccagag cggcagattt cgctcatctg ccgcagctag aatttggtgt atcaatgcga
      661 cctgcgttgt gtccatgcgg cctaacatag taagccctcc cagaatgtca gtaattagac
      721 tattaattga attgttacat ccaatagtga gctcccagtg attgtcttaa ctatccaacc
      781 tttcgattgg atggggcaga gcaccattat tctgtttgaa tgcggtgtta gtgtcaacta
      841 ggttctttat ttcttcaagg agtttgaaag tgctaagtat ttttacttta ctttgttcct
      901 caatataaat ttttttaaga ccatttagca gcttgtattt ggatattttg gataggagaa
      961 tgaataaggt tgcggtattg attcttcccc cttttagatg aagtaaacgg tttttttgca
     1021 gtattggaat atttctatat tctgttagtt gatcttttaa gtcatcagga attttcattt
     1081 cgtaaaaatt atatttttta gaacagtcta atatcgagtg atttaggctt tctgtatttg
     1141 tattaataaa aatgcatttt tcaaatgtgc aatttttaaa gcgacaatct ctaaaaatag
     1201 cagcataaaa tacgcacttt ttgaagtgaa cattcgtaaa cctattctta tttaagatta
     1261 ctcctgaaaa atcagataaa acaattttta tatttttgaa tgaacatgat cgtaggcggg
     1321 atcctctcaa attaacatta aaaaatgttt tattagagaa acgtacttta gaagaaatag
     1381 atttggctag gaggctattt gaatacttac gtttttcgat gtgcttgtta tgaaatctag
     1441 gaagttttga cattaatagt ccaaagaata ggagtttcag cttaattctt aggtgtaatc
     1501 aaaaaaaata atggacaggt ctcttgcgaa atctgtccat tagttttaaa ttcgatgggt
     1561 acagttattt gatgcttgca acatcaatta acgaaagatc ggcagagctt gcaactcata
     1621 ctgatattca ccttaaaggc tagtaccact atctatttta aagaattaga acagtttgac
     1681 aagcctcaat ttgaagtcct tcgttaattc agcagtttaa atcatcaaat aagcacctaa
     1741 cggtcgagct ttatctactt ctgtgcgcct tttttaagga agatatgcgt tgcatgtgga
     1801 gggaataagt gtcactggtg catcgtagcg atagtcgcaa ggctaaaccg cttacagggg
     1861 atctcacata ggcggagcgt tggaagtagt tgtataggtt ggttaagttg catgttggag
     1921 taatcctcgc agagatacca ccatacttcg acagggagcg cgtgggagaa ggtgcaaaac
     1981 ctttagtcct tgtctgcatg gtcagcaaaa ccatgtgcgg atacgaccga tgcgtggctc
     2041 cgtcagccca attacaggtt aatcggcaat acttaagagg atatttgatt tttttaaatc
     2101 aaagcctctt agggaaagta ttgccgaaac tctctcaacg ttcaccatca gcccgagcaa
     2161 agcgagggat gtggggacgg tgagaaagag ttgagcgcat ttgtttttac aagattcatg
     2221 ccaataccta ctagagttta tataccgtgt catttgatta gtttttattg aatttcactc
     2281 gtccattttt aaataagcaa ggatacacag ctacccctat ttaacttcgt taaacagtga
     2341 tagtctgtga ccttgatcag gggcttcacc cccgataccc ccgaaaagct ttaaacagaa
     2401 aaatcaaaga ttttccaatt taaagcttac aaaagccaaa gcagggtaga ttgaaatgtc
     2461 aggacaggaa caagaaaaca aacagagcaa agcagttcga gaacgaacct ttaaagtcag
     2521 actttcagaa tcagaatatc aattgctcga ttccaataat ccttatggtt cgattgcaaa
     2581 gttgcttaga caagcagcag tagagaaagc gactaaaatt cataattcca aagcagaaga
     2641 tgaccaaaaa attaaagcaa caaaggttgt tgaatcttcg tatagcaaga cagagcgagc
     2701 attaatttta gagcttggtc ggattggcaa caatgtgaat cagattgcta aagcagtgaa
     2761 taccgatatt gcaggggtag gtacttttga caaagtgaag ttacttcatt tgttgatttc
     2821 catagatcag cagcttcggg agttacgtcc agatgatcgt tgatttcttt agacatggtt
     2881 caggtttatc aaaaggctgt ctcgattatt tgttaggtga agatcgagag cgtgaacatg
     2941 ctcaagtgct caatggtgat gtagaactga cagcacaact cattgatagc agtccttttg
     3001 ccaaaaaata taccagtggt tgtttgtcct tttatgagca tgatttatct gaccaggata
     3061 aacagaaaat catgcaaaat tttgaggaat gtttattccc gggactagat aaagaccaat
     3121 atcaaatttt atgggtacag caccaagaca aaattaatca ggacacaggt gagacaaggc
     3181 ttgagcttaa ttttgtgatt cctaatgttg agctttcaac aggcaaacgc ctacagccat
     3241 tctatgcgcc agtcgattta gatcgagtcg atctatttaa gcagatcact aataccgaac
     3301 attcactgta tgaccccgat gatcctgagc atcgacagct atttttaaat aaaaagaacc
     3361 tacccaaaga tattaaggat tttaaagcgc agttacatca acgtgtttac cgagcggttg
     3421 ccaatggtga tgttgccgat cggcaagaat tggtgcaatg gcttgaatcg aatcaaatca
     3481 atgtcactcg acaagtaaaa aactccatat ccattgaaaa cccatatgag ggcgcaaaac
     3541 gtcccatccg tttagagggc gagatttatg agcaaggctt tagagctact ggcgaatata
     3601 gacaagaagt acaacaacga attgaagaat acagaggaac aacttctgag cggtatcgaa
     3661 caaacgtcac agactatcag agacagcttg aacataagag ccaatatcac tgcgaccgct
     3721 atccaacagt cggacgagaa aatagcccag agcattcaaa acaacgccca gacggtcgag
     3781 aagcagttaa gcctgtatca aaccttgctg caatcgaaat tgagccattt aacgcaatta
     3841 aacgagcaaa tacagagcca agaacaacaa gcccagaatc aagcggtaca gaaaaaactt
     3901 accactttga atatgggact gatttcagta gcagttattt tgcttatagc aattttctcg
     3961 cttggtctcg tcaccaaaaa caagtacagc gagatgcaga cgctaaatac cgtgatcaac
     4021 gagaagcaaa tgagggtcga ccatttgaac cgatcgggag gcagtttgac gatcagcaca
     4081 tgcaagcgac cagacagcaa agcacagcgc ctatgtattc agatcaacaa gaacgcaggc
     4141 gaatggcaga acggctacat gattccaatg gggtattaaa cgatgaccga attagaaaca
     4201 caattattga gaatcatcga agaacaacag caacaattaa tgcaacaaca agtgcaattg
     4261 cagaagcaac agcaagcttt agacacaatg caaaccaaaa ttatccaagc cttgtcagca
     4321 caatcaaaga caatcaagag cgatcagcaa agcttacagc aagcacagaa atcattagcg
     4381 acaatgcaga agcaatttca cgagctagaa cgaagtacag caagctatac cgagcagatc
     4441 aatggcaaag tccgagagat gagccttatc acagccaaca acgaagaaat tctggagaga
     4501 atctcaacca cacagcattc tgtctcgtca gaagtagcca aagcgcagca gaatttgctc
     4561 gaaacatccg agatattgaa aaaaatctcg tccagatgaa tgagcgtaaa caagagatag
     4621 aaaaacctcg acctaagcaa gatcgaggta tggattttgg gatgtaatta ttttctagta
     4681 tgttggtgtt tatttcttag tattagcttg tactaatgtt taaatttagc tatattgaat
     4741 gtagtatagg cttgtttata tattttggtg gtgttatgaa taataactgt gtagtagtac
     4801 taactgctcg tggaaagact agaattttaa aagaaggtgg tagtcagtct tggagactaa
     4861 atgctcataa tgcaggaaag tgcgattatc tagtttgtgt gcaaaatagg aaacaatctt
     4921 ggggagaacc agaggctgat catcacactg catttttagt tggcaaaata tctagtatca
     4981 gccaatctca agaagaagat gcagatggta gatggataat aaatatctcc gaatatgctg
     5041 atatatctat tcccaatatg tgggatggta atcggaatcc agtttcttat agttcattag
     5101 aagagatgaa tatcgatttg aatgagctga aatttaccaa attaaatgaa gataattctt
     5161 ctaaaacact ggataaaata tctattcctg aagctaagag actattagct aattattttg
     5221 atgtccctca agaaaatatt tcaattacaa tcaatatgta agcttttaat tgaccaaaaa
     5281 aagaaaaaag cctttcacgt taatgaaagg cttttttgaa tcttagcgct tgggagggaa
     5341 gatcttagat ccgcagtctc tcaaacttac aactatatat tagggtatat acggtggaat
     5401 tgtcaaatga tgcagtgctt aagatagagc aggctttatc tataccacgc ttatctaagt
     5461 atgaaaattt ctataaagat aaaggtgaac catatgaaaa atctgatgta ttgatgcttt
     5521 atgaacgtaa tttaataatt tccaataagt ttttttactt attaaattat tttgaagttg
     5581 ttctgagaaa tgctgttgtt caggcaatag aaatatcttt taggtgtaat gaaacaaata
     5641 gttggcatga aaatgaagct tttattcgaa gccttagccg tagagggagg tatagcccta
     5701 aaagtatgtt tgatagtgct aaagagaagt tcccagattc gcctagtaaa atgatccctg
     5761 agcttaaatt tgtattctgg caaaagatgc ttatggctaa ttatgaagaa cgtatttggc
     5821 agggatgttt taattctatt tttcctaatg caacagttga aaacaggcaa attttttacg
     5881 attggacaga tcaattacgt gaattaagaa acaggattgc tcatcatgaa ccgattattt
     5941 ttaaccgtaa tctagaaaat gatttagagg ttttaacaaa atttatttac tgtcgttgta
     6001 ttaatacata cgattttatc gagcaatctg cattagatct aaaagaatat ttagattttt
     6061 aagataagaa tttcaccc
//
