LOCUS       NZ_CP027181             6078 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter baumannii strain AR_0070 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP027181
VERSION     NZ_CP027181.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04014911
            Assembly: GCF_003431865.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            McAllister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2018) Bioinformatics, Institute for Genome
            Sciences, University of Maryland, Baltimore, 801 West Baltimore
            Street, room no 646, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.6.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 22.16x
            Sequencing Technology             :: PacBio;Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 20:57:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,262
            CDSs (total)                      :: 4,165
            Genes (coding)                    :: 4,025
            CDSs (with protein)               :: 4,025
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 51 of 140
            Pseudo Genes (incomplete)         :: 82 of 140
            Pseudo Genes (internal stop)      :: 32 of 140
            Pseudo Genes (multiple problems)  :: 21 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP027181.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="AR_0070"
                     /db_xref="taxon:470"
                     /plasmid="unnamed3"
     gene            complement(4..672)
                     /locus_tag="CSB70_RS20890"
                     /old_locus_tag="CSB70_0001"
     CDS             complement(4..672)
                     /locus_tag="CSB70_RS20890"
                     /old_locus_tag="CSB70_0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     gene            complement(805..1290)
                     /locus_tag="CSB70_RS20895"
                     /old_locus_tag="CSB70_0002"
     CDS             complement(805..1290)
                     /locus_tag="CSB70_RS20895"
                     /old_locus_tag="CSB70_0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     gene            complement(1399..3213)
                     /locus_tag="CSB70_RS20900"
                     /old_locus_tag="CSB70_0003"
     CDS             complement(1399..3213)
                     /locus_tag="CSB70_RS20900"
                     /old_locus_tag="CSB70_0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3203..3610)
                     /locus_tag="CSB70_RS20905"
                     /old_locus_tag="CSB70_0004"
     CDS             complement(3203..3610)
                     /locus_tag="CSB70_RS20905"
                     /old_locus_tag="CSB70_0004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4613..5299
                     /locus_tag="CSB70_RS20910"
                     /old_locus_tag="CSB70_0005"
     CDS             4613..5299
                     /locus_tag="CSB70_RS20910"
                     /old_locus_tag="CSB70_0005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     gene            5390..5923
                     /gene="aadB"
                     /locus_tag="CSB70_RS20915"
                     /old_locus_tag="CSB70_0006"
     CDS             5390..5923
                     /gene="aadB"
                     /locus_tag="CSB70_RS20915"
                     /old_locus_tag="CSB70_0006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     ARG             5391..5920
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             5379..5923
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          complement(1403..3213)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atcttaaaaa tctaaatatt cttttagatc taatgcagat tgctcgataa aatcgtatgt
       61 attaatacaa cgacagtaaa taaattttgt taaaacctct aaatcatttt ctagattacg
      121 gttaaaaata atcggttcat gatgagcaat cctgtttctt aattcacgta attgatctgt
      181 ccaatcgtaa aaaatttgcc tgttttcaac tgttgcatta ggaaaaatag aattaaaaca
      241 tccctgccaa atacgttctt cataattagc cataagcatc ttttgccaga atacaaattt
      301 aagctcaggg atcattttac taggcgaatc tgggaacttc tctttagcac tatcaaacat
      361 acttttaggg ctatacctcc ctctacggct aaggcttcga ataaaagctt cattttcatg
      421 ccaactattt gtttcattac acctaaaaga tatttctatt gcctgaacaa cagcatttct
      481 cagaacaact tcaaaataat ttaataagta aaaaaactta ttggaaatta ttaaattacg
      541 ttcataaagc atcaatacat cagatttttc atatggttca cctttatctt tatagaaatt
      601 ttcatactta gataagcgtg gtatagataa agcctgctct atcttaagca ctgcatcatt
      661 tgacaattcc accgtatata ccctaatata tagttgtaag tttgagagac tgcggatcta
      721 agatcttccc tcccaagcgc taagattcaa aaaagccttt cattaacgtg aaaggctttt
      781 ttcttttttt ggtcaattaa aagcttacat attgattgta attgaaatat tttcttgagg
      841 gacatcaaaa taattagcta atagtctctt agcttcagga atagatattt tatccagtgt
      901 tttagaagaa ttatcttcat ttaatttggt aaatttcagc tcattcaaat cgatattcat
      961 ctcttctaat gaactataag aaactggatt ccgattacca tcccacatat tgggaataga
     1021 tatatcagca tattcggaga tatttattat ccatctacca tctgcatctt cttcttgaga
     1081 ttggctgata ctagatattt tgccaactaa aaatgcagtg tgatgatcag cctctggttc
     1141 tccccaagat tgtttcctat tttgcacaca aactagataa tcgcactttc ctgcattatg
     1201 agcatttagt ctccaagact gactaccacc ttcttttaaa attctagtct ttccacgagc
     1261 agttagtact actacacagt tattattcat aacaccacca aaatatataa acaagcctat
     1321 actacattca atatagctaa atttaaacat tagtacaagc taatactaag aaataaacac
     1381 caacatacta gaaaataatt acatcccaaa atccatacct cgatcttgct taggtcgagg
     1441 tttttctatc tcttgtttac gctcattcat ctggacgaga tttttttcaa tatctcggat
     1501 gtttcgagca aattctgctg cgctttggct acttctgacg agacagaatg ctgtgtggtt
     1561 gagattctct ccagaatttc ttcgttgttg gctgtgataa ggctcatctc tcggactttg
     1621 ccattgatct gctcggtata gcttgctgta cttcgttcta gctcgtgaaa ttgcttctgc
     1681 attgtcgcta atgatttctg tgcttgctgt aagctttgct gatcgctctt gattgtcttt
     1741 gattgtgctg acaaggcttg gataattttg gtttgcattg tgtctaaagc ttgctgttgc
     1801 ttctgcaatt gcacttgttg ttgcattaat tgttgctgtt gttcttcgat gattctcaat
     1861 aattgtgttt ctaattcggt catcgtttaa taccccattg gaatcatgta gccgttctgc
     1921 cattcgcctg cgttcttgtt gatctgaata cataggcgct gtgctttgct gtctggtcgc
     1981 ttgcatgtgc tgatcgtcaa actgcctccc gatcggttca aatggtcgac cctcatttgc
     2041 ttctcgttga tcacggtatt tagcgtctgc atctcgctgt acttgttttt ggtgacgaga
     2101 ccaagcgaga aaattgctat aagcaaaata actgctactg aaatcagtcc catattcaaa
     2161 gtggtaagtt ttttctgtac cgcttgattc tgggcttgtt gttcttggct ctgtatttgc
     2221 tcgtttaatt gcgttaaatg gctcaatttc gattgcagca aggtttgata caggcttaac
     2281 tgcttctcga ccgtctgggc gttgttttga atgctctggg ctattttctc gtccgactgt
     2341 tggatagcgg tcgcagtgat attggctctt atgttcaagc tgtctctgat agtctgtgac
     2401 gtttgttcga taccgctcag aagttgttcc tctgtattct tcaattcgtt gttgtacttc
     2461 ttgtctatat tcgccagtag ctctaaagcc ttgctcataa atctcgccct ctaaacggat
     2521 gggacgtttt gcgccctcat atgggttttc aatggatatg gagtttttta cttgtcgagt
     2581 gacattgatt tgattcgatt caagccattg caccaattct tgccgatcgg caacatcacc
     2641 attggcaacc gctcggtaaa cacgttgatg taactgcgct ttaaaatcct taatatcttt
     2701 gggtaggttc tttttattta aaaatagctg tcgatgctca ggatcatcgg ggtcatacag
     2761 tgaatgttcg gtattagtga tctgcttaaa tagatcgact cgatctaaat cgactggcgc
     2821 atagaatggc tgtaggcgtt tgcctgttga aagctcaaca ttaggaatca caaaattaag
     2881 ctcaagcctt gtctcacctg tgtcctgatt aattttgtct tggtgctgta cccataaaat
     2941 ttgatattgg tctttatcta gtcccgggaa taaacattcc tcaaaatttt gcatgatttt
     3001 ctgtttatcc tggtcagata aatcatgctc ataaaaggac aaacaaccac tggtatattt
     3061 tttggcaaaa ggactgctat caatgagttg tgctgtcagt tctacatcac cattgagcac
     3121 ttgagcatgt tcacgctctc gatcttcacc taacaaataa tcgagacagc cttttgataa
     3181 acctgaacca tgtctaaaga aatcaacgat catctggacg taactcccga agctgctgat
     3241 ctatggaaat caacaaatga agtaacttca ctttgtcaaa agtacctacc cctgcaatat
     3301 cggtattcac tgctttagca atctgattca cattgttgcc aatccgacca agctctaaaa
     3361 ttaatgctcg ctctgtcttg ctatacgaag attcaacaac ctttgttgct ttaatttttt
     3421 ggtcatcttc tgctttggaa ttatgaattt tagtcgcttt ctctactgct gcttgtctaa
     3481 gcaactttgc aatcgaacca taaggattat tggaatcgag caattgatat tctgattctg
     3541 aaagtctgac tttaaaggtt cgttctcgaa ctgctttgct ctgtttgttt tcttgttcct
     3601 gtcctgacat ttcaatctac cctgctttgg cttttgtaag ctttaaattg gaaaatcttt
     3661 gatttttctg tttaaagctt ttcgggggta tcgggggtga agcccctgat caaggtcaca
     3721 gactatcact gtttaacgaa gttaaatagg ggtagctgtg tatccttgct tatttaaaaa
     3781 tggacgagtg aaattcaata aaaactaatc aaatgacacg gtatataaac tctagtaggt
     3841 attggcatga atcttgtaaa aacaaatgcg ctcaactctt tctcaccgtc cccacatccc
     3901 tcgctttgct cgggctgatg gtgaacgttg agagagtttc ggcaatactt tccctaagag
     3961 gctttgattt aaaaaaatca aatatcctct taagtattgc cgattaacct gtaattgggc
     4021 tgacggagcc acgcatcggt cgtatccgca catggttttg ctgaccatgc agacaaggac
     4081 taaaggtttt gcaccttctc ccacgcgctc cctgtcgaag tatggtggta tctctgcgag
     4141 gattactcca acatgcaact taaccaacct atacaactac ttccaacgct ccgcctatgt
     4201 gagatcccct gtaagcggtt tagccttgcg actatcgcta cgatgcacca gtgacactta
     4261 ttccctccac atgcaacgca tatcttcctt aaaaaaggcg cacagaagta gataaagctc
     4321 gaccgttagg tgcttatttg atgatttaaa ctgctgaatt aacgaaggac ttcaaattga
     4381 ggcttgtcaa actgttctaa ttctttaaaa tagatagtgg tactagcctt taaggtgaat
     4441 atcagtatga gttgcaagct ctgccgatct ttcgttaatt gatgttgcaa gcatcaaata
     4501 actgtaccca tcgaatttaa aactaatgga cagatttcgc aagagacctg tccattattt
     4561 tttttgatta cacctaagaa ttaagctgaa actcctattc tttggactat taatgtcaaa
     4621 acttcctaga tttcataaca agcacatcga aaaacgtaag tattcaaata gcctcctagc
     4681 caaatctatt tcttctaaag tacgtttctc taataaaaca ttttttaatg ttaatttgag
     4741 aggatcccgc ctacgatcat gttcattcaa aaatataaaa attgttttat ctgatttttc
     4801 aggagtaatc ttaaataaga ataggtttac gaatgttcac ttcaaaaagt gcgtatttta
     4861 tgctgctatt tttagagatt gtcgctttaa aaattgcaca tttgaaaaat gcatttttat
     4921 taatacaaat acagaaagcc taaatcactc gatattagac tgttctaaaa aatataattt
     4981 ttacgaaatg aaaattcctg atgacttaaa agatcaacta acagaatata gaaatattcc
     5041 aatactgcaa aaaaaccgtt tacttcatct aaaaggggga agaatcaata ccgcaacctt
     5101 attcattctc ctatccaaaa tatccaaata caagctgcta aatggtctta aaaaaattta
     5161 tattgaggaa caaagtaaag taaaaatact tagcactttc aaactccttg aagaaataaa
     5221 gaacctagtt gacactaaca ccgcattcaa acagaataat ggtgctctgc cccatccaat
     5281 cgaaaggttg gatagttaag acaatcactg ggagctcact attggatgta acaattcaat
     5341 taatagtcta attactgaca ttctgggagg gcttactatg ttaggccgca tggacacaac
     5401 gcaggtcgca ttgatacacc aaattctagc tgcggcagat gagcgaaatc tgccgctctg
     5461 gatcggtggg ggctgggcga tcgatgcacg gctagggcgt gtaacacgca agcacgatga
     5521 tattgatctg acttttcccg gcgagaggcg cggcgagctc gaggcaatag ttgaaatgct
     5581 cggcgggcgc gtcacggagg agttggacta tggattctta gcggagatcg gggatgagtt
     5641 acttgactgc gaacctgctt ggtgggcaga cgaagcgtat gaaatcgcgg aggctccgca
     5701 gggctcgtgc ccagaggcgg ctgagggtgt catcgccggg cggccagtcc gttgtaacag
     5761 ctgggaggcg atcatctggg attactttta ctatgccgat gaagtaccac cagtggactg
     5821 gcctacaaag cacatagagt cctacaggtt cgcatgcacc tcactcgggg cggaaaaggt
     5881 tgaggtcttg cgtgccgctt tcaggtcgcg atatgcggcc taacaattcg tccaagccga
     5941 cgccgctttg cggcgcggct taactcaggt gttaggaaac actgctaccg ttatcggatt
     6001 tactgcttta ctactaacct tgttaattca acatttgcaa aatagtctga cctttcgatt
     6061 gtttggggtg aaattctt
//
