LOCUS       NZ_CP027141             4604 bp    DNA     linear   CON 22-APR-2024
DEFINITION  Enterobacter hormaechei subsp. hoffmannii strain AR_0365 plasmid
            unnamed4.
ACCESSION   NZ_CP027141
VERSION     NZ_CP027141.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07291508
            Assembly: GCF_002968455.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei subsp. hoffmannii
  ORGANISM  Enterobacter hormaechei subsp. hoffmannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            Mcallister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-Kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            Mcallister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-Kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Sring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 2.3.0; HGAP v. 3.0; SPAdes v. 3.6.0
            Genome Coverage                   :: 16.64x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002968455.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 03:18:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,233
            CDSs (total)                      :: 5,117
            Genes (coding)                    :: 5,009
            CDSs (with protein)               :: 5,009
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 29 of 108
            Pseudo Genes (incomplete)         :: 79 of 108
            Pseudo Genes (internal stop)      :: 22 of 108
            Pseudo Genes (multiple problems)  :: 19 of 108
            Pseudo Genes (short protein)      :: 1 of 108
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP027141.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Enterobacter hormaechei subsp. hoffmannii"
                     /mol_type="genomic DNA"
                     /strain="AR_0365"
                     /sub_species="hoffmannii"
                     /db_xref="taxon:1812934"
                     /plasmid="unnamed4"
     gene            complement(655..2211)
                     /locus_tag="CSC02_RS00005"
                     /old_locus_tag="CSC02_0001"
     CDS             complement(655..2211)
                     /locus_tag="CSC02_RS00005"
                     /old_locus_tag="CSC02_0001"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013199864.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_045326453.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLD
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVFV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPFKSVTGNLIILLSHPIYD
                     KNGNYIGHVGGSIYLKKHSLFSDVLSRHFFKSDTEISIVSDDGTVIFNNDNSAVGRPMV
                     MSDDLKSKLTESDRGEGEFFFSGNRYLLGYAHMKNTAWNVLVYSHADSATTILITYAKS
                     VLVLLIVIIITFSLLSYFIASRISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTHVKVMMERVDSLNEVALKDPLTGVNNRLGFSTKIQNNKYGAGASVIAIDVDFF
                     KKINDSFGHGVGDEVLVSLAQIIKSCCRSEDIICRFGGEEFVVFLPNTSVVTAECVAER
                     IRSVIERTVFPNNLRVTVSAGVATQDDPLGGIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     gene            complement(2557..2772)
                     /locus_tag="CSC02_RS26910"
     CDS             complement(2557..2772)
                     /locus_tag="CSC02_RS26910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015589619.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2776..3261)
                     /locus_tag="CSC02_RS26915"
                     /old_locus_tag="CSC02_0004"
     CDS             complement(2776..3261)
                     /locus_tag="CSC02_RS26915"
                     /old_locus_tag="CSC02_0004"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_045326458.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<3296..4000)
                     /locus_tag="CSC02_RS27505"
                     /pseudo=""
     CDS             complement(<3296..4000)
                     /locus_tag="CSC02_RS27505"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3939..4262)
                     /locus_tag="CSC02_RS00015"
                     /old_locus_tag="CSC02_0005"
     CDS             complement(3939..4262)
                     /locus_tag="CSC02_RS00015"
                     /old_locus_tag="CSC02_0005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(4376..4548)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.126"
                     /coverage="100.0"
                     /evalue="1.99e-77"
     Typing          2..358
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.593"
                     /coverage="80.0"
                     /evalue="1.54e-148"
     Typing          complement(2454..3949)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3946..4262)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="2.47e-61"
ORIGIN
        1 gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca
       61 gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gtggctccct
      121 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc aatccgctgt tatggccgcg
      181 tttatctcat tccacgcctg acactcggtt ccgggaaggc agttcgctcc aagctggact
      241 gtatgcacga accccccgtt cagcccgact accacgttgt cccggtaact atcatcttga
      301 gtccaacccg gaacagacac gaaaaatcgc cagtggcggt agccattagt aagaggattc
      361 acggagcgga gtagtagagt gttctacaga gttcttgaag tggtggcctg actgcggcta
      421 cactggaagg acagttttgg tacctgcgct ccgtgaaagc cagttacctt cggaagaaag
      481 agttggtagc tcttgattcc ggcaaacgaa ccaccgttgg tagcggtggt ttttttgttt
      541 gcagggcatg agcttacgac gacatcgaag ggataacaag aggatccgct tcttgaaccc
      601 ccctccccac aaaaaaaaac ggcacactag cgcgtaaagc gtcatcaaaa aaactcaaat
      661 agcgctatat acaatcactt tattacgccc ctctctctta gcttgataaa gagcatcgtc
      721 agcatttttc agcaaaaaat cgatcccacc aagaggatca tcttgtgttg ccacaccagc
      781 agatacggtg acacgcaagt tattagggaa aacagtacgt tcaataacac tcctaatacg
      841 ctccgcaaca cactccgctg ttaccacaga tgtattcggc agaaaaacaa caaactcttc
      901 tccaccaaac cgacaaataa tatcttcact acggcaacaa gacttaataa tctgagccaa
      961 agaaacaagc acctcatctc cgaccccatg cccgaaacta tcatttattt ttttaaaaaa
     1021 atcaacatca atagcaataa ctgaagctcc tgccccatac ttgttattct gtattttagt
     1081 tgaaaacccc aatctattgt tcacgccggt aaggggatct ttaagcgcca cctcatttaa
     1141 agaatcaacc ctttccatca ttaccttaac atgcgtaaaa agtgcctttt taagtcgatc
     1201 agcttcggca taccaagcat tgatagcttt aatataaact aacgaagccc ctatatcttt
     1261 agcattggta gatatagcaa gcttttctaa aggaagtgaa atacgggagg caatgaaata
     1321 tgaaagaagt gaaaatgtaa taattattac aattagcaga acaagtacac ttttagcata
     1381 agtaataaga atagtagtcg cgctgtcagc atggctataa accaacacat tccaagcagt
     1441 atttttcata tgagcataac ccagaagata tctattcccc gaaaaaaaaa attccccctc
     1501 acctctatca gactccgtta actttgattt caaatcatca gacataacca tgggccgacc
     1561 aacagcactg ttatcgttat taaagatcac agttccatca tcactaacaa tgctaatttc
     1621 agtgtcgctc ttgaaaaaat gcctgctcaa aacatcgcta aataaactat gtttttttag
     1681 atatatagac ccaccgacat gccctatgta attgccattc ttatcataaa taggatgtga
     1741 caacaaaata atcaaattcc cagtaactga tttgaacggt gaagatatga atggtttctt
     1801 ttcctcaatc gccaatttgc tggcacttga attcaagcgg gtaccaacta aatcaagaga
     1861 ttctggcgaa gtcgccagaa caacagcatc attactaaca acaaaaactg aattaaacat
     1921 accagactgc aatcttaacc tatccgcctc atctttaagt gagtcaatat cattagttga
     1981 ttttattttt tttgctccat atgctaattc accttgagcc atttcaatat aaccactcat
     2041 tacatctgac attttcatag cgtacgatga attagcatca agcaaatctt tttctatatt
     2101 ttcgcgctga aaaaccataa taacggcaat cagaaaaata gaagttaaca aaatcccacc
     2161 aacggacaag gttatgacta tcggacgaag gtgaggcttt ttaattctca taaaaataca
     2221 ctccatataa taaaaaatgc cgcatttacc ataacatccc ttatcagcac cgccccggac
     2281 gggttcggaa caaatgcggc gtcagcccgg taaaaccggc agcaaactct ccgctaaacc
     2341 gcgcattctg cgccagataa atttcacccg gctgcatggc tatgcagtcg ggtgaatggc
     2401 tccggctttc agcgcagatg tccaccgaaa gccccgtttt atcaggttat cacagtgaag
     2461 ggctgttata cgtccgctgt cgctgagatc gctccagctc cagcgctttc tgatgctgca
     2521 ccacgcgctc ctgctcccgg tgcagctcga cggcattcag tcgctcgaca accggcgcag
     2581 ctgctcgctc aaactctgca cctgcctgct caagcccgtg acgcgctcgc tcagcgctgc
     2641 gttctcccgt tgcataagac cagacatcgt ccgccattcc gcgaaggcgc tctcccactc
     2701 gtccagcctt ttcgagtagt cctgctgtag ctgctctaat gcgctcagca actgtttttc
     2761 cagctccgtc atgtgctatt tccccgccag tatcatccag ctcgaatcct ctccgaaccg
     2821 tcccgcttcc gggttcacct tcacgcagcg gcgtctctgc tcgccgcagg tcgagagctg
     2881 cacgccgctg ttcctctctg acagcatggc ctgcgtgctc ttctgctccc ggatggtcgt
     2941 gtagttgtcg agtatctgct gcccctgcca ccacagaatg cccgcgctcg aggcgatcag
     3001 cagcgcggag accaggacga tggtcagcca cgtctggctg accatgcgca ccatgccttt
     3061 cgtgcgctgg ctcatggctg aggacagttt ccggtcgtgg tcgaggatgg cggcgctgat
     3121 tctcttcgcg ctttcgttca gttccgcttt tacagatttc tcgtgctcgc tgaatgcggc
     3181 tttcagcatc tcgccggtac tctgctgctg cgctttcgat ttctgctcta agtccttcgc
     3241 cagcgttaaa agactgttca taaatggctc ccttcagtcg gatgtttcgc cccccgtccg
     3301 ggtcggcaat gctgatactg ctttttgtgg tgcggaccac ctcaaaacct gcgctttcca
     3361 gcgcctcagt gacatcctga cgggttttca gctccccgga tgaggcaaga gcaagcagac
     3421 cgcgtgtaat cgcttctgca gcctcctgct tcgctttagg cagtgcagac ggtgtgacca
     3481 gtgcccgccg gttctccggt gcgttcgggt cgtgcagccc cagtctgccg ttcacgatgg
     3541 tctgccaggc atcgatgcgt ggacggtcgg cgcgatcgta atacggctgg agccgtctgc
     3601 cggtcagcag ctccgtgttc gggatcagaa aattcagctc aagccgcccc ttgtcacgat
     3661 gctccaccca caggatgctg tactggtctt tatcaagtcc gggcatcaga acccgctcaa
     3721 agcttgccat cagcttttcc cgctggccgg agggtaaatc cgcttccgca aacgacagta
     3781 ccccggaggt gtatttctta acgtaaggcg acgcgtcaat cagctcccgg acttcttccg
     3841 gcttcccctg caggacggtg gcgccttcac gctgccggtc tttacccagc agatagtcca
     3901 ccggcccggc gccaccgccg cgaccgcgtg gatgaaattt aacgatcatc gtccgttcct
     3961 ttctcaagaa cggcgtgccg cagccgctca agtccggcat cgatggccat cagcgccgca
     4021 acaatctgca ccctgtcatg gccggtaccg ccgccggcgt taacacggcg ggctatctgg
     4081 ttcaggttat tgcccatgcc ggcaagctgg cgcagcagcg ccggcgagat tgagggaagc
     4141 tttccggaac gtgccggctt ttcgtccagg caggtctgcc gcatccacgc tgccagctgc
     4201 ctgccgtcgc agcgctcaag cagccgccgg tgctcgtcct ccgtcaccca catggtgagc
     4261 atcttgttcc gtttctctga caaaccggcc tcctgataac gccgggacgg gcgggaaagc
     4321 ctttcccggt cggcgcgggg ttggacaagc cgcaggcgcg tcaggctttt ggcgggtttc
     4381 ggggcgcagc cctgaaccag tcacgtagcg ctagcggagt gtatactggc ttactatgtt
     4441 ggcacggatg cgagtgtcag tgaaatgctt catgtggcag gagaaaaatg cagcagcgga
     4501 gcgtcagcag aatatgtgat acaagatata ttccgcttcc tcgctcactg actcgctgcg
     4561 ctcggtcgtt cggctgcggc gagcggtacc ggcttacata caga
//
