LOCUS       NZ_CP027132             2101 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain AR_0372 plasmid unnamed2, complete sequence.
ACCESSION   NZ_CP027132
VERSION     NZ_CP027132.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07291515
            Assembly: GCF_002968735.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            Mcallister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-Kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            Mcallister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-Kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Sring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 2.3.0; HGAP v. 3.0; SPAdes v. 3.6.0
            Genome Coverage                   :: 14.00x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002968735.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 03:29:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,370
            CDSs (total)                      :: 5,240
            Genes (coding)                    :: 5,017
            CDSs (with protein)               :: 5,017
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 83 of 223
            Pseudo Genes (incomplete)         :: 151 of 223
            Pseudo Genes (internal stop)      :: 45 of 223
            Pseudo Genes (multiple problems)  :: 48 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP027132.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AR_0372"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
     gene            complement(802..1068)
                     /locus_tag="CSC09_RS01235"
                     /old_locus_tag="CSC09_0001"
     CDS             complement(802..1068)
                     /locus_tag="CSC09_RS01235"
                     /old_locus_tag="CSC09_0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(1072..2037)
                     /locus_tag="CSC09_RS01240"
                     /old_locus_tag="CSC09_0002"
     CDS             complement(1072..2037)
                     /locus_tag="CSC09_RS01240"
                     /old_locus_tag="CSC09_0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(1413..1644)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 tgcgagccag tacatgtcac cagagtttgc cgctgaggtg acacattcga aatgttgggg
       61 agtgttgacg cactccccaa cgccgattaa aaacgcctca attctgctcc attttctttg
      121 acctgtcaaa acccactcaa agcctcgtca ttactgggct aaaccactat ttcatatccg
      181 ccatttatcc cttaagtata aatacaagga tcacgcgcga gaattgcgca aaaaataacc
      241 acaattttaa ccccccaaaa caagcacccc cgaacggctt accccaacct tttccacaca
      301 cccgatcacc aaaacaggga ataaaaacaa aaccctgtct tacaggtgga tggcctcact
      361 tcgtgaaacc atccaccccg cataagtttt ttaatcggtg gtgtcgtcaa tcactaaatc
      421 ggaaccccaa aggggacccc cacgatttag catttgacga gattaactct cggagatggc
      481 atcatgcttt taacggactt taaaaaacgg tgaacggatg cggattttaa tccgtgtctg
      541 tgaggcgttg agcaacgagc aaagcgaggc ggtagaaaga gagccagcgg caggctcagg
      601 acgataatcc ggggaaaatg taccactcgc agagtgttaa catttgacat cggattgtct
      661 atcctgcatt ggtgtcgagt tggcaaggct gaggataatg ctgaggataa tgctgaggat
      721 aatgcaggat agacagagca ctagagattg catgcaatct cgtacctatc acctcagcct
      781 gccgctggct cttttccaga actaagaatg cgttcttaaa aatccaaact tgataccgtt
      841 taaatctatt ttcacaaaaa gagtttttac cctctcgttt gttttctgaa tatataaaat
      901 taattcatcc tcaaaacctt cgtgcggatg aaaaccaaat gtgccaaaac ttaatccatc
      961 aataagcaaa ataaggtttt taaacctgtc aggaatcacc gcttcacttc ttaaaaaaaa
     1021 taaaccaata gtcttatttc cattcaggta ttcaatatta atattcatac gttaatccga
     1081 tttatccttc tcaggggcgc gtttgtaacg ccgtttacct gaatcccaga caaacgccgt
     1141 ccgcttcccg tcatcagtgc catcccctac atcgtcggca atgacaagat cctcattggt
     1201 ttctcgatcc aactggagga cgttttttag cgccccaccg gtcgagataa aacggcgctt
     1261 gtgaagctgc cgcgtcagct caagaaacca ctcaggatcg tttgccatat cttccggttt
     1321 aacggagtat ttcagcgttt cagcaaccgc gctttgcagt tgctcggcaa cgttggccac
     1381 aacctcacct gtcttagttt ttactgcccg aatatcgata ttcggctcat agttcacccg
     1441 caagcaatcg cgccagagtt ctacccaacg ttcgtgctta acgtagttct tccctttaaa
     1501 ccaagaaggt tgcaccatca gcagacagtg aaaatgcgga tgtgcgctgc catccttacc
     1561 tcgcgtcacc tccgtagccc tgatccaccc ctgaacaggt gatagctcct ttcgcttttc
     1621 cattcgctta aacgccgcat tcattgctgt aaggactgtt ccaagttcac ctatctcgca
     1681 gttcctgaca gttaacgtca gaaacaacca tcgggaagac gggtaatcca caacgatttt
     1741 cggtagagcc tgataaaaac gggcttgcca catgagggtt cttctccact ggcagacagg
     1801 gcaatgacgg acacggcaaa attgcgcact acgaagccgt aggcgcgttt ctccggtttc
     1861 catgatggta gaccaaccaa aacgtaacaa accgctacag gaggccatac ggagtgcata
     1921 gcgctcgaat tcagcagcag tctggtagat acccccgaca gaatcggtaa gcgtgcgctg
     1981 ggtatcccaa actgcatcac gggggctgta gtccgaaagg aatttatctt ctgacattaa
     2041 gcctgctccg atctgggcag gctgtgattt gatgtggtat attcatcacg tacctgtgca
     2101 t
//
