LOCUS       NZ_CP027131             1552 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain AR_0372 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP027131
VERSION     NZ_CP027131.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07291515
            Assembly: GCF_002968735.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            Mcallister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-Kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            Mcallister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-Kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Sring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 2.3.0; HGAP v. 3.0; SPAdes v. 3.6.0
            Genome Coverage                   :: 14.00x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002968735.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 03:29:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,370
            CDSs (total)                      :: 5,240
            Genes (coding)                    :: 5,017
            CDSs (with protein)               :: 5,017
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 83 of 223
            Pseudo Genes (incomplete)         :: 151 of 223
            Pseudo Genes (internal stop)      :: 45 of 223
            Pseudo Genes (multiple problems)  :: 48 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP027131.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AR_0372"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
     gene            381..857
                     /locus_tag="CSC09_RS01215"
                     /old_locus_tag="CSC09_0003"
     CDS             381..857
                     /locus_tag="CSC09_RS01215"
                     /old_locus_tag="CSC09_0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(194..302)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.95e-50"
     Typing          1451..1536
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.954"
                     /coverage="95.0"
                     /evalue="4.39e-26"
     Typing          complement(1088..1348)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.511"
                     /coverage="100.0"
                     /evalue="3.95e-106"
ORIGIN
        1 tgaacggacg gcaaaaaacg tgcaggagct gttgaacgtg aaaaccacga aatctggcaa
       61 aaatcacggc tggcgaggct tgcagaggag taagccagta tacactccgc tatcgctacg
      121 tgactgggtc agggcttcgc cccgacaccc ccaaagggcg ttgcgttgcg cgcaacaccc
      181 ttgcctagaa tagatctttt acgccgattt gtaagttgtt gtttttttag tgtgtttttt
      241 gtttacctgt cgcatccagg gtgacacaac gttgtcgcat cctggatgcg acaacacgca
      301 acaaggtgtt gcacagatgt gtcatgatgg cttagatttg aaccaacgtt agtgcatggg
      361 atttttagag ggaaaaaatc atgtttattg attcagaaaa acgactgaaa caactttcag
      421 atgaggcaaa gaaaaacacc gaggatctcg aagaagcaaa gaagaattca aggtttacac
      481 aggtatcccc aaaaggttgg gaacgcgttc gagagctgct gaaggatagc caaggcatat
      541 cagcactgaa gctgtactca tttttagcgg agcatatcga tcctacgtgt ggcgctgtcg
      601 ttgcggatca gcaattccta gctgaaaaac ttggagttag cagaagcaca attattcgtt
      661 ggctcaatta cttagaatca aaaaatgcat tagttagaat ccccgttgct ggtaaggttt
      721 gtgcgtatgc cctcgatcca catgaagtct ggaagggata caacactacg aaaaaccatg
      781 cagcatttgt cactaaaaca ctggtcaaca aagacggtga tattcagcgc cgaatcatgg
      841 ccatgttttc aaattgagcc agcggcaggc ggacaatcag gggttacgtg ttaacgttct
      901 gaccatgatt gtctatcctg cattgctctt ttgccgcctc aaaatccctt gcgtgttttt
      961 gctccccgat ctccagaacg accgagccgc cacggttccg gcagcgcctt gagcgcaacc
     1021 ggaaaccgtc tggggagaac cccagacccc catcgctgta acaccgacgg tctacgacca
     1081 cctaccaacc gagtgactgc ctgtaccgtg caaatgggct ttgcaggggg ctggcgcggt
     1141 tcctggcgac ctgaaagccc ctggctaggg gtatcggcgc gcctgacctc ctgaaacgct
     1201 ctgtaagcgt ttttcgcgcc ttccatgccc tgacgggcat ttaaaccccg cctcgtgcgt
     1261 ctgagagctt ctgagcgcgt ttgaagggct atctgacgac tcatccccga aagaacaaca
     1321 gcggaatcac cgacaacatg cgagaggtgg caccgctcgc cgcagcccca gcgaccgaac
     1381 gttagtgagc gagtgggcga ggaagcggaa gagaactgga aaccacattg agcacgtacg
     1441 cactgatacg gggtgtcggg gcgaagccct gaccagatgg caaacggaat atcgtcgcgg
     1501 gtgacggtat tacatttgca catcctgtcc cgatttctgc gggggttaac gg
//
