LOCUS       NZ_CP027128             1565 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain AR_0374 plasmid unnamed1, complete sequence.
ACCESSION   NZ_CP027128
VERSION     NZ_CP027128.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07291517
            Assembly: GCF_002999075.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            Mcallister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-Kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            Mcallister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-Kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Sring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 2.3.0; HGAP v. 3.0; SPAdes v. 3.6.0
            Genome Coverage                   :: 18.16x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002999075.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 03:23:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,870
            CDSs (total)                      :: 4,751
            Genes (coding)                    :: 4,571
            CDSs (with protein)               :: 4,571
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 63 of 180
            Pseudo Genes (incomplete)         :: 118 of 180
            Pseudo Genes (internal stop)      :: 32 of 180
            Pseudo Genes (multiple problems)  :: 31 of 180
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP027128.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AR_0374"
                     /db_xref="taxon:562"
                     /plasmid="unnamed1"
     gene            928..1407
                     /locus_tag="CSC11_RS26015"
                     /old_locus_tag="CSC11_0001"
     CDS             928..1407
                     /locus_tag="CSC11_RS26015"
                     /old_locus_tag="CSC11_0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000275450.1"
                     /translation="MYIGTEEQRKKRLKELEKLNKESESDPKNNGPFTQVYQKGWERIR
                     ELSKDKQGVVAIPLYSFLAEHIDPSCGAVVADQQFLADKLGVSRSTIKRWLNYLESKNA
                     LVRIPVAGKVCAYALDPHEVWKGYNTSKNYAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          468..548
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.341"
                     /coverage="87.0"
                     /evalue="2.0600000000000002e-29"
     Typing          complement(93..353)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.275"
                     /coverage="100.0"
                     /evalue="1.83e-109"
ORIGIN
        1 ttccggcagc gccttgagcg caaccggaaa ccgtctgggg agaaccccag acccccatcg
       61 ctgtaacacc gacggtctac gaccacctac caaccgagtg gctgcctgta ccgtgcaaat
      121 gggctttgca gggggctggc gcggttcctg gcgacctgaa agcccctggc taggggtatc
      181 ggcgcgcctg acctcctgaa acgctctgta agcgtttttc gcgccttcca tgccctgacg
      241 ggcatttaaa ccccgtttcg tgcgtctgag agcttctgag cgcgtttgaa gggctatctg
      301 acgacttatc cccgaaagaa caacagcgga atcaccgaca acatgcgaga ggtggcaccg
      361 ctcgccgcag ccccagcgac cgaacgttag tgagcgagtg ggcgaggaag cggaagagaa
      421 ccggaaacca cattgagcac gtacgcactg atgcggggtg tcggggcgaa gccctgacca
      481 gatggcaaac ggaatatcgt cgcgggtgac ggtattacat ttgcacatcc tgtcccgatt
      541 tctgaggcgt tttagcggtg aacggacggc aaaaccatga tggagctgtt gaacgttgag
      601 agcgtgaaat ctggcaaaaa tcacagctgg taaggcttgc agaggagtaa gccagtatac
      661 actccgctat cgctacgtga ctgggtcagg gcttcgcccc gacaccccaa aaaagtgttc
      721 cgctttgcgc aacactttca gcagaaaaga tatttaaggg cgattttgta agttattgat
      781 tttttagcta cttgcattat gggttagctc acgcatgtgt ggtctaaggt tagctcacgc
      841 atgtgtgagc taggtcgacc tatcatggtt tacgtttgag ctatgatggc ttatatttga
      901 gccatcatta gaccgtggag atttaccatg tatatcggga ctgaagagca gcgaaaaaag
      961 cgtctaaaag aactggaaaa attaaacaaa gaatcggaat cagatccgaa aaataacggc
     1021 ccattcaccc aggtttatca gaagggctgg gaaaggatcc gagaactgtc gaaggacaag
     1081 cagggagttg tagcaatacc gctttactcg tttttagctg agcacataga tccctcatgc
     1141 ggtgctgttg ttgccgatca gcagttttta gcagataaat tgggtgttag ccgaagcaca
     1201 attaagcgct ggctcaacta cttagaatca aaaaacgcgt tagtcagaat tcctgttgct
     1261 ggtaaggttt gtgcgtatgc actcgatcca catgaagtct ggaagggata caacacatct
     1321 aaaaactacg cggcgtttgt cactaaaaca ctggtcaaca aagacggtga tattcagcgc
     1381 cgaatcatgg ccatgttttc aaattgagcc agcggcaggc acaggcaaaa accccgtttt
     1441 tggacaatct gattcaaatg ttaacatttg acatcgggtt gtctatcctg cattgctctt
     1501 ttgccgcctc aaaatccctt gcgtgttttt gctccccgat ctccagaacg atcgagccgc
     1561 cacgg
//
