LOCUS       NZ_CP027124             8731 bp    DNA     circular CON 19-DEC-2023
DEFINITION  Acinetobacter baumannii strain AR_0056 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP027124
VERSION     NZ_CP027124.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04014897
            Assembly: GCF_003029495.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            Mcallister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-Kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     FDA/CDC Antimicrobial Resistant Isolate Bank Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8731)
  AUTHORS   Benahmed,F.H., Lutgring,J.D., Yoo,B., Machado,M., Brown,A.,
            Mcallister,G., Perry,A., Halpin,A.L., Vavikolanu,K., Ott,S.,
            Zhao,X., Tallon,L.J., Sadzewicz,L., Aluvathingal,J., Nadendla,S.,
            Voskania-Kordi,A., Simonyan,V., Patel,J. and Shawar,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Sring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 2.3.0; HGAP v. 3.0; SPAdes v. 3.6.0
            Genome Coverage                   :: 900x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/19/2023 03:45:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,121
            CDSs (total)                      :: 4,027
            Genes (coding)                    :: 3,904
            CDSs (with protein)               :: 3,904
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 33 of 123
            Pseudo Genes (incomplete)         :: 86 of 123
            Pseudo Genes (internal stop)      :: 11 of 123
            Pseudo Genes (multiple problems)  :: 7 of 123
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP027124.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="AR_0056"
                     /db_xref="taxon:470"
                     /plasmid="unnamed3"
     gene            303..764
                     /locus_tag="C7R87_RS20725"
                     /old_locus_tag="C7R87_0001"
     CDS             303..764
                     /locus_tag="C7R87_RS20725"
                     /old_locus_tag="C7R87_0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(1087..1260)
                     /locus_tag="C7R87_RS20960"
                     /old_locus_tag="C7R87_0002"
     CDS             complement(1087..1260)
                     /locus_tag="C7R87_RS20960"
                     /old_locus_tag="C7R87_0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282484.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQN"
                     /name="hypothetical protein"
     gene            complement(1260..1631)
                     /locus_tag="C7R87_RS20730"
                     /old_locus_tag="C7R87_0003"
     CDS             complement(1260..1631)
                     /locus_tag="C7R87_RS20730"
                     /old_locus_tag="C7R87_0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            complement(1903..2157)
                     /locus_tag="C7R87_RS21215"
     CDS             complement(1903..2157)
                     /locus_tag="C7R87_RS21215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014303.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     gene            2841..3791
                     /gene="repM"
                     /locus_tag="C7R87_RS20745"
                     /old_locus_tag="C7R87_0004"
     CDS             2841..3791
                     /gene="repM"
                     /locus_tag="C7R87_RS20745"
                     /old_locus_tag="C7R87_0004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            3784..4359
                     /locus_tag="C7R87_RS20750"
                     /old_locus_tag="C7R87_0005"
     CDS             3784..4359
                     /locus_tag="C7R87_RS20750"
                     /old_locus_tag="C7R87_0005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            4379..4522
                     /locus_tag="C7R87_RS20965"
                     /old_locus_tag="C7R87_0006"
     CDS             4379..4522
                     /locus_tag="C7R87_RS20965"
                     /old_locus_tag="C7R87_0006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(4711..5229)
                     /locus_tag="C7R87_RS20755"
                     /old_locus_tag="C7R87_0007"
     CDS             complement(4711..5229)
                     /locus_tag="C7R87_RS20755"
                     /old_locus_tag="C7R87_0007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            5602..5889
                     /locus_tag="C7R87_RS20760"
                     /old_locus_tag="C7R87_0008"
     CDS             5602..5889
                     /locus_tag="C7R87_RS20760"
                     /old_locus_tag="C7R87_0008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            5876..6190
                     /locus_tag="C7R87_RS20765"
                     /old_locus_tag="C7R87_0009"
     CDS             5876..6190
                     /locus_tag="C7R87_RS20765"
                     /old_locus_tag="C7R87_0009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            6318..8729
                     /gene="znuD2"
                     /locus_tag="C7R87_RS20770"
                     /old_locus_tag="C7R87_0010"
     CDS             6318..8729
                     /gene="znuD2"
                     /locus_tag="C7R87_RS20770"
                     /old_locus_tag="C7R87_0010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     Typing          2842..3791
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagatactgt aatcttctaa gaaaatttct tagaagattt ttcttataaa atgattagat
       61 taataattaa ggtaagcgtc cgctgaatcc catacctatt ttttaattcg atttaggttt
      121 ccagcagtgt ggcagtaact cttcaatctg ggtcacttta tgtgtcggca gccttttcag
      181 cactgaatag ccaccgattt tgtagacacc ttttagctag ataaaaagca atttttttga
      241 tctttaaaga atgaggatat aagctgttta aaacttaatt ctaattggtt aataggtgaa
      301 aaatgaaatt agcagaagct ctcttacttc gaagtgatca acaaaaaaaa attatttcac
      361 taaaacaacg tatcaatgca aatgtattgg tacaagatgg tgatcaacca tctgaagatc
      421 caaatgaact actaaaacaa gtattttctt taattcaaga atttcaaaaa ttaagttatg
      481 caattcatga aactaatgct ttaacaaaat taaatgatgg ccgttctcta cttgctttat
      541 tgactttgcg tgatgaattt gtagaacagc acaaaaccct aactgcagca ataagtaata
      601 catctcgtga atctgatcgt tatagtacgc gggaaataaa gtggcataaa gttattccag
      661 tttcatcatt acaaaaacaa gctgatgata ttagtcttaa attacgtgat ttaaacgtgt
      721 taattcagtc gactaattgg aaaattgatt tgattgaagc ctaatagtcc tttagatata
      781 atcaagttga acatactggg cgagtacaag atcccttact tccacaacat aggattgtaa
      841 ataaagtgta gtggcttgca aagcaagtgg ataagcttaa aattttaagt acaaatcaat
      901 ccatcacttg gcataactca aatctttgtg caaacaaagt tacacgttac ttataactac
      961 catgtactga cagtatgttc acctcaatta ctaaaaataa agaaatgaac aagcatcagc
     1021 tcaactacat ttctattttt aattcagttc ggcatttaac ataaaatctc ttatacgaag
     1081 cccaggttaa ttctgggctt tttgttcaat ctcatcattg gccgagaaca tacttggatt
     1141 cgtaagcctg atattttcct gacaacttcc acacagcatc aatcctgcaa aaagttgtac
     1201 attttgtcta ttttcacaaa gcgtacatgg ctttgaaagc tgaataatat tatctgacac
     1261 tactttaccc tcttatagtt atcatctgca tatgaataac ctttagcttg tatcattcta
     1321 acctcatctt tagaagcgcc atttttcatt aagagccaca acatcatgaa atatagtcca
     1381 gaaaagtctt catagtattt ttcagaaaaa ttgttatctt cctcaaaatc atctattcgc
     1441 ttttctaatc gattcaccag atcctcaata cgatcggctt gctgtttaac agcaatagat
     1501 tgaaaatcat tggtcaaaat tttactgaat aatttactgg ttctgattcc ttctgaatta
     1561 gccaattgat cgtagaattc tgctacttgt ggatctaacc tgactgcata agttttgact
     1621 ttttcgccca taaattacac ctcttattaa aattagattt tgtacaaaat taaatatata
     1681 taattcaact aattaaataa aaaataataa ttagattacg tacaaactta tttgtacaga
     1741 attaatttat tctaaaacaa aaacttattt aaatataatg ctttttttat gtacaatcat
     1801 gagtacatat tttttcttaa atttatcaat atcttatcta aaaaaagtgt tgttttgatg
     1861 tacaaccatg agcacagcat aaaaaataga taattcaatc acttattcta aaaaaagacc
     1921 atttttctgt acacgtttct gagaccctaa aaaatcgctg aaatccttat cctgtaaggc
     1981 tttgaggggt gtcatcgtta gatgcgtggt gtgtgacaaa aaaatcccgg catgtgccgg
     2041 atcttggatt ggaaaattgg ctaaagtgac gtagggccgg tgcttggttt tgcatggaaa
     2101 aaagtattta tttccttgtt tagaaaaacg taaaaaatca attttttgtc cattcatcca
     2161 ggttaaaaat ttcaacccaa aactttaatt atgaaaagct tcatagaacg cgttcaaatg
     2221 cgatttaaga gcctttacct agaaaacata gatctcatag tgaaaaacac aaaacagctc
     2281 aaaaaacgca aatgagagcg aaataaagag gtattttgac tttagataga tgcatagagc
     2341 gagtgtctac gagcgaacta tcaaaatttg cgcctagacc ctctgaaaaa catttttttg
     2401 tcctctttag cctaagaaag cgtaagtttc gtgcagaaat ttgctcctgg atcgagcgta
     2461 gcgagtaaaa aagttttatg agcgaagcga attccaagtt tcttttgctt ttttttaaag
     2521 tcacgccagt attaaccaaa aaattgcccc aacgaactga gcgaaagcga agttcaatag
     2581 agtttgagcg aagcgaaaac caagggcaat ttttcattcc ctgggctttt aattatttta
     2641 aagtttttaa atacttttaa atggcttgaa agccttatga ataaagggtt ttagctctta
     2701 tatgtccacg tttaccttgc aatatgtcca cgtttacctt gcaatatgtc cacgtttacc
     2761 ttgcaatatg tccacgttta ccttgcatta gtacacaaat aatattaacg tgtacttata
     2821 cacaataaaa aatagtggct atgagagatt tagttgtaaa ggacaatgcc ttaatcaacg
     2881 caagctataa cttagactta gtagaacaac gtttaatttt attggctatt gttgaagcaa
     2941 gggaaagtgg gaaagggatt aatgcaaatg atccattaga agttcatgca gatagttata
     3001 tcaatcaatt tggtgtacac cgtaatacag cttatcaagc cttaaaagat gcttgtaagg
     3061 atttattcgc gcgtcaattt agctatcaag agaaaaaagc taatgggaat atccgaaatg
     3121 ttatgagtcg ttgggtatct caaattgctt ataacgacaa tgaagcaact gtagacttaa
     3181 tatttgcacc tgctgttgtt cccttcataa cccgactgga agaacaattt actaaatatg
     3241 aattacagca agttagtagt cttagtagcg cttatgccat tcgcttatat gagcttctaa
     3301 ttcagtggcg aagcactggt aaaaccccaa ccatagaact acaagaattt agaaagaagt
     3361 taggcgttct tgataatgaa tatttacgga tggctcattt aaaagagcgt gttttagagc
     3421 tttcaattaa acaaataaat gagcatacgg atataactgt aaaatatgaa cagcataaaa
     3481 gaggacgttc tatttcagga ttttctttta cctttaaaca gaagaagaag gatagcccat
     3541 caatagaaag agatccgaac actttggagc ttttttcaaa gatgaccgat gctcaacggc
     3601 atatgtttgc aaataaactt tcagaactcc ctgaaatggg tcgctattca caaggaacag
     3661 aaagctacca acagtttgct gtacgtattg ctgagatgct acaagatccc gctcaattca
     3721 aagagctata cccataccta aaaaaagtgg gatacatgcc atcaaataaa aaggacaccg
     3781 taaatggcta agttatcact aagtgaagta tctaaaaaat ttcatgtgga tagatcaacc
     3841 atttacagag ctgtacgtaa tggacgttta tcacgctcca gtgatggaca attcgatcta
     3901 gcagaggtca tacgatgctt tggagaacct gagcaaacat ctcaaaaaat tgaatcatct
     3961 aagcaagaag gtgatgaatc tacaaaaaaa cttattgccc atttagaaaa tgaagtcaaa
     4021 aaataccaag aacgtgaaga acggttaatg caacaaattg accgtatgca aacactcatt
     4081 gagctgaaaa gtgttgcacc tgccacagca gcaccacacc aagatgctac ggcatgcgac
     4141 accaagatgc cacagcatgc gacaacacaa caagacactg ataacaaaaa gaataatgaa
     4201 ttaaatattg cagaaaatgt agcagtgcca cagcaagaaa ctacggcata ccacacccaa
     4261 acgctacagc atgccacgtt gcaaagtgtg gcagtgccac aacacaaaaa acgtggttta
     4321 tttggccgtg tgctgaatgc cgtctttgat aatgactgag caataagggt gaaagatcat
     4381 gccgaaactg aaagacattg ccctgggaat tattgtagct ccgctgctga tcccgatcat
     4441 gctgattgca tcgtaccagg ataaaaaggc gctgaagaaa gagctggatg aatgccaaaa
     4501 agaaaaagac caggcatcct gattattcaa ttgagctaat caaaagtgtg gaacattgat
     4561 gaaataatag cgtggaacgt gaaaagtcat aaaaaaagcc cgaacaggga tgctcgggct
     4621 ataaactaga aactacagcg ttgaattata aggataatta tacagcattt cgtataaggt
     4681 gtattatgtt aattttagga aatattaaaa ctaattttca gacttatccc accaactttg
     4741 agctaaatca gtatttttag gaatacctat accatcacga tacataacgg aaagacttcg
     4801 cattgctttc ttattaccta actttgcagc cttttcacga agttcaaaag ctttattaat
     4861 attaacttct attccgctat ctgcacgagt atatagatca gctaactcaa ccattgattc
     4921 agaatggcca ttttctgagg ctttctgaaa ccacttaaaa gctttttgga aatgttctaa
     4981 agaatcgttt ttgtcatcat cttccattaa tctagcataa gtaacatatc catcagctaa
     5041 ccaaaacatt gctgctacat ttccattttc tgctgattta agagcccaaa cttcagcttg
     5101 attttcatct gaatcaacac catgaccata tagataaatc tcggctaatt ctaactgagc
     5161 agaagtatct ccattttctg ctttcgctaa taaatcatta gaaaaaacag gttctgcaaa
     5221 tgtttccatt gaaaggcaaa ctcctaaagc cataaaaata tttgttaatt tattcaattt
     5281 ttaaccttta aactaaactg ttaggcttgt atatttttat atcataccta aaagaaaact
     5341 ctaaaattag agtatgtctt atctctattt aacataatgg cgattatacg aagtacactt
     5401 gtatattaat ataaatatca actacttaat aaaaatagaa aaatcccaaa atgcccaaaa
     5461 agccgacttt gaaacaagtc ggctttttta tgagcagttt tgatacttct tgccaataat
     5521 tttgactaaa aattacacga atctggttga gtgatacaat tttgtgatac attattgtat
     5581 atacaaattt gaatgttttt tatggaacag tattttgaat gggatgaggc taagaatcga
     5641 aagaatcaga aaaagcatga tatctctttc gaaactgcaa gccttgtttt tgaagatcca
     5701 ttacggatct caatccaaga cagacatacc aatggtgaag aacgttggca aaccattgga
     5761 cgagttaaag gcgtactaat gttattggtg gctcacacca tttttgatga agatgactgt
     5821 gaaatcatcc gaatcattag tgcaaggcaa gtcactaaag cggagcgaaa caaatatgag
     5881 catggttaga tactcacaca aagagctgaa tgagaaattt ggtgagaagc aagatgctga
     5941 aattcaacgc ttgcttgcta aaggcacggt tcctgatgat cagctagatc tatcagatat
     6001 tcctgaaatt acagactgga gcaatgctgt acgccaaaat caattttacc gcccagtaaa
     6061 gcaacaaact tcaattcggt tagatgctga tgtacttgct tggttcaaag cacaaggcaa
     6121 aggttatcaa acacgtatga atgaaatttt acgagatgct atgctaaaag aattaaaaaa
     6181 tcatcaataa gaatgggagc ttaggctccc atttttgact tcatttttag gggggattac
     6241 cattcaaaaa tttaaatagt agattttata aatttttaag tgttatatta taatataaca
     6301 ttaaatttgg ttgttttatg ctaaataaaa gtaaactatt tttagcttta attactttag
     6361 gggcctcgaa aattttattg gcagctgaag ggcctgttac taccttaaat acaatagtat
     6421 taactgcaca atctgatgag ttagggtctg agctgttagg caaaagtcta aatgttagta
     6481 atcagtttat tgatactagt aaacttaaac agcgttcgac aactctaggg gatgccttgg
     6541 gtacggaatt aggaattcat tctaatcaat atggaggtgg agcatctact cctattattc
     6601 gtgggcagga aggaaaacgt attaaagttt tacagaataa tgctgacgtg ttggatatgt
     6661 ctaatatgtc cccagatcac gctgtcacag ttgaaccaag tttagctaag tctattgaaa
     6721 ttatacgtgg tgcaagtacc cttttgtata gttcaaatag tgcggcaggg gtagtcaatg
     6781 tcattgacta taaaattcca acccaaatgc ctcaagacgg attagagggt aatactacat
     6841 tacgttttaa tactggcagt aatgagaagt tgaccacagc aggtgtaaca gttggcttaa
     6901 gcccacatgt agctttacgg gcagaagggt tatatcgtaa tgctggtaac tataaaactc
     6961 ctcattacca gtctagctct tataattctt tagaagatct tgaaaaccaa aacagtattt
     7021 ataaaaattt aaaatattta ccagaaagct gggcagagtc gagagtaggt acgcttggat
     7081 tatcctggat agacgataat acttatttag gagtttctta tacacatcgt catgatgaat
     7141 atggtttacc agcccatagc catttatatg aaggttgtgg tgcctctgca atcggtatcg
     7201 attcacgaat tagtggatta aaaaattatc ttttatatta tcctcaattg atggatgagc
     7261 aagatattaa ttacataaat ccacgtcctg attgtcatca gcataatcat attcatgaaa
     7321 ctaatttctc tcataatgct ccttatattg atttaaatac tcgacgttat gatgtgcgtg
     7381 gagaattcac tcaaccattt accggtattg acaaaattcg tacctcattg agttatatag
     7441 attattttca caatgaactc gagggagata agattactaa cttttttaaa aatacaggaa
     7501 aagtaggccg catagagtta tcacatcagc cattagggga attaaccggt atcttggggc
     7561 tacaatattt ggaacaggat aatagtgctt tatcaccagt ccattctcaa gaaggccata
     7621 ccacctatct agataatcag cagctattaa atcgaaatgt cactaaaaac tttagtgtat
     7681 ttggcctaga aaaatataac tggaacgact ttactttcga actaggtgct cgtatagaaa
     7741 agcagaaagt tagtatggat tatgatatag aaaaaattaa agattcaatg aaaccttggc
     7801 ctaataaata taacagtcct tatgtagaaa aaaataataa aatcagggct caaaatttaa
     7861 aatctatttt agaagctgtt caacctaaca aagaaacagc tttttcttat gcaggtacag
     7921 tccactggag atttgctcca aattatatct tatcattaac aggtacacat caagaaagat
     7981 tacctaatgc acaagaaatg tatactcatg gtatgcatct cgctactaat tcttttgaaa
     8041 taggtaaccg ctttctaaga aaagagaaat ccaataattt agaaatcagt ctggcctata
     8101 aggatgattt acttgattat caaatcagta cttactatta tgattttgat aactatatat
     8161 atttacagac actcaatgaa gttttaggaa ctacaaaagt tagagatcag catacactac
     8221 gtattaatca ttatagccag tctgctgcta atttttatgg attagagggc aacatagggt
     8281 atcaatttaa ttctgtttat cacggctctc tttttggaga ttatgtgaaa ggccgtttaa
     8341 caaatttacc tgatgctgta attgcatacg atatctggaa tagagaaccg actttagctc
     8401 cccaaaagga tcgttacact cctagattac ctcctgctcg tttgggtaca cgactaaaag
     8461 ctgattttga tgaatcgctt aaaggtgaaa tagaatatta tcgagtcttt aaacaagata
     8521 atatttctaa atttgagcag gttacatcag gttataatat gcttaatatg actttagcat
     8581 ataaaaataa gctatctcac acagaatatg atttattctt taaagcgaat aatttacttg
     8641 atcagaaagt ctatgcccat gaaacatttt tgccatatat tccccaaatt ggaagaaatt
     8701 ttagtttagg cttaaatctt aatttctaga t
//
