LOCUS       NZ_CP027113             5523 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain BW plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP027113
VERSION     NZ_CP027113.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08554725
            Assembly: GCF_009930935.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Wood,R., Raygoza Garay,J.A., Mwangi,M. and Read,A.
  TITLE     Whole genome sequencing of an Enterobacter cloacae infection that
            evolved carbapenem resistance in a human patient
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Wood,R., Raygoza Garay,J.A., Mwangi,M. and Read,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2018) Biology, Penn State University, W-230A
            Millennium Science Complex, University Park, PA 16802, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-FEB-2018
            Assembly Method :: Canu for assembly of PacBio reads v. 1.6; Pilon
            for correction with Illumina reads v. 1.22
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: PacBio; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009930935.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 04:03:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,062
            CDSs (total)                      :: 4,948
            Genes (coding)                    :: 4,867
            CDSs (with protein)               :: 4,867
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 25 of 81
            Pseudo Genes (incomplete)         :: 58 of 81
            Pseudo Genes (internal stop)      :: 10 of 81
            Pseudo Genes (multiple problems)  :: 12 of 81
            Pseudo Genes (short protein)      :: 1 of 81
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP027113.1.
            Bacteria and source DNA available from Robert Wood.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="BW"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: Ann Arbor, Michigan"
                     /lat_lon="42.2845477 N 83.7296668 W"
                     /collection_date="2014-05-30"
                     /collected_by="Robert Wood"
     gene            complement(203..334)
                     /locus_tag="C5I89_RS25520"
                     /old_locus_tag="C5I89_25825"
     CDS             complement(203..334)
                     /locus_tag="C5I89_RS25520"
                     /old_locus_tag="C5I89_25825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_059686908.1"
                     /translation="MHEPPVQSDCCACSGNYHLESNPERHDKTPLAAATGKGCTRYY"
                     /name="hypothetical protein"
     gene            983..1306
                     /locus_tag="C5I89_RS25105"
                     /old_locus_tag="C5I89_25830"
     CDS             983..1306
                     /locus_tag="C5I89_RS25105"
                     /old_locus_tag="C5I89_25830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_172971213.1"
                     /translation="MLTMWVTDDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLDKGRD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1515..>1949
                     /locus_tag="C5I89_RS25430"
                     /pseudo=""
     CDS             1515..>1949
                     /locus_tag="C5I89_RS25430"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1984..2469
                     /locus_tag="C5I89_RS25115"
     CDS             1984..2469
                     /locus_tag="C5I89_RS25115"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_057060552.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAILSERNSGVQLSTCGEQGRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            2473..2688
                     /locus_tag="C5I89_RS25120"
     CDS             2473..2688
                     /locus_tag="C5I89_RS25120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137421.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2892..3443)
                     /locus_tag="C5I89_RS25125"
                     /old_locus_tag="C5I89_25840"
     CDS             complement(2892..3443)
                     /locus_tag="C5I89_RS25125"
                     /old_locus_tag="C5I89_25840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058999046.1"
                     /translation="MKDMKAVVVFTGKDLNIMRTEGGSGYWHARTDRLNDAEYLIAVRN
                     RRETWAVKDLEHGTAFLIAKITGCFKNPDYDDRNVITFDEYAEIHSPDAWKMLTGGQRY
                     PVAYLNAQEAFLRIGVTPEQLEWKKFHPSSPSLPNAVVPSLAEEKTEKLSLNEAIERAK
                     KDISNATGIDSSAITISIKI"
                     /name="hypothetical protein"
     gene            3842..4027
                     /locus_tag="C5I89_RS25130"
     CDS             3842..4027
                     /locus_tag="C5I89_RS25130"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_109553065.1"
                     /translation="MTEDNVKNISDYRKKNDAVDANGSAVSSKNAPARLSEKLRNAIPS
                     GLRTQMRQQMRNKHGL"
                     /name="hypothetical protein"
     gene            4017..4274
                     /locus_tag="C5I89_RS25135"
                     /old_locus_tag="C5I89_25845"
     CDS             4017..4274
                     /locus_tag="C5I89_RS25135"
                     /old_locus_tag="C5I89_25845"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_057060549.1"
                     /translation="MGFNNDQNKDLQDKQADLLDQTYTLGWMDGYSEGYDDAIDDSQNA
                     LNKLENENELLNEIIEKKDNIINEMASGVGKALQTVKKNK"
                     /name="hypothetical protein"
     gene            complement(4303..5361)
                     /locus_tag="C5I89_RS25140"
                     /old_locus_tag="C5I89_25850"
     CDS             complement(4303..5361)
                     /locus_tag="C5I89_RS25140"
                     /old_locus_tag="C5I89_25850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508929.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_071949222.1"
                     /translation="MAIYAVWNNKGGVGKSYLTFQLASEYARQNPTKKVLVIDLCPQAN
                     SSSMLLGGMVNGENTLNQIHSATQRKTISGYIEDRIRSPYIPTNTGSNYVINVSTYNSR
                     IPNNLYLVTGDEQLELQASRVVGASFPGPHDAWRTVHLWINDLISDICNSWDNDDSCVF
                     IDCNPSFSIYTELALTAAERLLIPFSADGSSKRAVKAVLSLVYGERRHQNEPQSDFYLN
                     SNRYSMSLPQIYMYVGNRLTQMNNSSASAFKTVVTEIGDEIWKVWQTKPSLFCIHPNGN
                     STPNNKKSFRNMFQYEVNDANTASVVSGALGIPIATLTAGNKNVSGRTIKVNQTQLDKQ
                     VPNIVALAGMIE"
                     /name="ParA family protein"
     Typing          697..869
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.103"
                     /coverage="100.0"
                     /evalue="9.410000000000001e-66"
     Typing          complement(167..772)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.388"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1297..2792
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.184"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgaaaggatc tcttgagatc cttagtcgtg ggatgtaatc ttttgctccg taaacgaaaa
       61 aaccacctgg ggaggtggtt tttcgaagga tcagtaagtt ggggaacttc tgaaccgtgg
      121 taacagggtg tacaagaccg ctgccaccaa atctgtcctt tcagtttagc tgtagttagg
      181 cttcaacttc aagaactctg cgttagtaat atcttgtaca tcccttacca gtggctgctg
      241 ccagtggcgt tttgtcgtgt ctttccggat tggactcaag atgatagtta ccggaacagg
      301 cgcagcagtc ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct
      361 tcccggaact gagtgtctgg cgtggaatga gagaaccgcg gccataacag cggaatgaca
      421 ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg agccatcagg gggaaacgcc
      481 tggtatcttt atagtcctgt cgggtttcgc caccactgat ttgagcgtca gatttcgtga
      541 tgcttgtcag gggggcggag cctatggaaa aacggctccg ccgtggcctt atcgcttccc
      601 cgttaagttt cttcctggca tctcctagga aatttccgcc ccgcctgtaa gccggtaccg
      661 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaatatatcc
      721 tgtatcacat attatgctta cgctgcgctg ctgcatttct ctcctgccac atgaagcact
      781 tcactgactt ccgcacctgt gccaacatat taagccagta tacactccgc tagcgctgcg
      841 tgactggttc agggctgcgc cccgaaaccc gctaaaacca ctgacgcgcc tgcggcttgt
      901 ccaacaccgc gccgaccggg aacagatttc ccgcccgtcg cggcgttatc aggaggccgg
      961 attggcagac aaacgcagca aaatgctcac gatgtgggtg accgacgatg agcaccgccg
     1021 tctgctggaa cgctgcgacg gtaaacagct tgcggcctgg atgcggcaga cgtgcctgga
     1081 cgagaagccc gcccgcgccg gcaaacttcc ctcgatctcg ccggcgctgc tgcggcagct
     1141 cgccggcatg ggtaataacc tgaaccagat agcccgccgg gttaatgccg ggggcggtac
     1201 cggccatgac agggtgcaga ttgtggcggc gctgatggcc atcgatgccg gactggagcg
     1261 cctgcgtcat gctgtgctgg ataaggggcg ggatgatgat cgttaaattt catccccggg
     1321 gacgcggagg tggttccggg ccggtggatt accttctggg aaaagaccgg cagcgcgagg
     1381 gcgccagtgt gcttcaggga aaaccggagg aagtccggga gcttatcgac gcctcgccct
     1441 acgccaaaaa atacacctcc ggcgtcctgt cctttgccga aaaggattta ccgcccggcc
     1501 agcgtgaaaa gctgatggcg agcttcgagc gggttctgat gcccggactt gataaagacc
     1561 agtacagcgt gctgtgggtt gagcaccagg acaaggggcg gctggagctg aacttcctga
     1621 tcccaaatac ggagctgctg accggcaggc gtctccagcc gtattacgac cgcgccgacc
     1681 gtccgcgcat cgatgcctgg cagaccatcg ttaacggcag gctgggactg cacgacccga
     1741 acgcgccgga aaaccggcgg gcgctggtca cgccgtccgc gctgccggaa gcgaagcagg
     1801 aagcagctca ggcgattacg cggggtttac tggccttagc ctcgtccggg gagcttaaaa
     1861 cgcgtcagga cgtcactgag gcgctggaaa gcgcaggttt tgaggtgatc cgcaccacaa
     1921 aaagcagcat cagcattgcc gacccggacg gggggcgaaa catccgactg aagggagcca
     1981 tctatgaaca gtcttttaac gctggcgaag gacttagagc agaaatcgaa agcgcagcag
     2041 cagagtaccg gcgagatgct gaaagccgcc ttcagcgagc acgagaagtc tgtcagagcg
     2101 gaactgagcg aaagcgagaa gagaatcagc gccgccatcc tcgaccacga ccggaagctg
     2161 tcctcagcca tgagccagcg cacgaaaggg atgctgcgca tggtcagcca gacgtggctg
     2221 accatcgtcc tggtctccgt cctgctgatc gcctcgagcg cgggcattct gtggtggcag
     2281 gggcagcaga tactcgacaa ttacacgacc atccgggagc agaagagtac gcaggccata
     2341 ctgtccgaga ggaacagcgg cgtgcagctc tcgacctgcg gagagcaggg acgccgctgc
     2401 gtgagggtga acccggaagc gggacggttc ggagaggatt cgagctggat gatactggcg
     2461 gggaaatagc acatgacaga gctggaaaaa cagttgctga gcgcattaga gcagctacag
     2521 caggactact cgaaaaggct ggacgagtgg gagaacgcct tcgcggaatg gcggacgatg
     2581 tctggtctta tgcaacggga gaacgcggcg ctgagcgagc gcgtcacggg cttgagcagg
     2641 caggtgcaga gtttgagcga gcagctgcgc cggttgtcgc aaggctgaac tgtgtcgagg
     2701 cacaccggga gcaggcgcgc gcggcgcagc accagaaagc gctggagctg gaacgatcgc
     2761 agcgacagcg gacgtacaac ggtccgtcac tttgaatctg tgcgccggaa aagcatgagc
     2821 ttacttctgg cgcacagatt cagttctcgc atgttagaac gtgaggctgc tatcctgttg
     2881 ttcacgctgc atcagatctt gatgcttatt gttatggctg agctatcaat tcctgttgcg
     2941 ttgctaatat cctttttggc tctttcaatt gcctcgttaa gggagagttt ttctgttttc
     3001 tcttcagcca gactaggaac cactgcatta ggaagcgatg gggatgaagg atggaatttc
     3061 ttccattcca gttgttccgg tgttacccca atacgtaaaa aggcttcctg tgcgttcagg
     3121 taagcaacag gatagcgctg accaccggtt agcattttcc atgcgtctgg agagtgaatt
     3181 tctgcatact catcaaaggt tatgacgttc ctgtcatcgt aatcgggatt tttaaagcac
     3241 ccggtaattt ttgcaatcag gaaggctgtg ccgtgttcca ggtcttttac tgcccacgtt
     3301 tcccttctgt ttcttactgc tatgaggtat tcggcatcgt taagcctgtc tgttctggca
     3361 tgccagtagc ctgacccccc ctcagttctc attatgttca aatcttttcc ggtaaaaaca
     3421 acaacagcct tcatatcttt catatagaaa tccttactag gtgaatgaat ctcatttttt
     3481 acttaatcta tacgatagaa tttaaggtat caataatttt atgcgataaa atctcgatca
     3541 ttgcctgttt taccgttagt ggtaagactc tctgctccga gtttttgatg aatcaggtaa
     3601 aatagggctt taggtgaaga tctacattga aagcctgagc cattcacccg gctgcatggc
     3661 tatgcagtcc ggggaaacta atcaggacgg attttcgcgg tttcgggcag agaaagggat
     3721 gccttttggc ccggacgcgc taagatcgcg ctgagggcct ttgcgggggt gcgggcaagg
     3781 gatgttatgt tcaattctcc agtgtatatt gggttattca cagcgatagg agtagtgttc
     3841 tatgactgaa gataacgtta aaaatattag tgactacaga aaaaagaatg atgctgttga
     3901 tgctaatggt agcgctgttt caagtaaaaa tgctccagcc aggctgagtg agaaacttag
     3961 aaatgcaatt ccttctggac tgagaactca aatgcgccaa caaatgagga acaaacatgg
     4021 gctttaataa cgatcagaat aaagacctac aagataagca agctgacctt ctggaccaga
     4081 cttatactct tggctggatg gatggttatt ctgaaggata cgatgatgcc attgacgata
     4141 gtcagaatgc tttgaataaa ctggaaaatg aaaacgaatt actcaatgaa atcattgaaa
     4201 aaaaagataa tataatcaat gaaatggcgt ccggtgtagg caaggcgtta caaacagtaa
     4261 agaaaaacaa ataaaaaaac aggccctttc aagggcctgt ttttactcaa tcataccggc
     4321 aagagctaca atgttgggaa cttgcttatc aagctgtgtt tgatttacct ttattgttct
     4381 tcctgaaacg tttttatttc ctgcggttag cgtggcaatt ggaatcccaa gtgctcctga
     4441 tacgacagat gctgtatttg catcattcac ttcatactgg aacatgttcc taaatgattt
     4501 tttattattc ggcgttgagt ttccatttgg atggatgcag aacagacttg gttttgtttg
     4561 ccacactttc caaatctcat cgccaatctc tgttaccaca gtcttaaatg cgctagcaga
     4621 tgagttgttc atttgggtta atctattacc tacatacatg tatatttgcg gtaatgacat
     4681 agagtagcga ttgctgttta aatagaaatc tgactgtggt tcgttctgat gacggcgctc
     4741 gccataaact aaagaaagaa cagctttaac tgctcttttt gatgaaccat cagcagagaa
     4801 tggaattaag aggcgttctg ctgcggttaa cgctagctca gtataaatag agaagctagg
     4861 gttgcagtcg atgaacacac agctgtcgtc attatcccat gagttacaaa tgtcactaat
     4921 tagatcgtta atccaaaggt gaactgttcg ccatgcgtcg tgaggccctg ggaatgatgc
     4981 tccaacaact cttgatgctt gaagttctaa ttgttcatcg ccagtaacaa gatatagatt
     5041 atttggtatt ctgctattgt atgtagatac gttgattacg tagttagaac ctgtgtttgt
     5101 cggaatgtat gggcttctga tcctatcctc aatatatcca gaaatagtct ttctttgtgt
     5161 cgcagaatga atttgattta gcgtattttc gccattaacc atcccgccaa gtaacattga
     5221 ggatgagtta gcttgcgggc atagatcaat caccagaacc ttttttgtcg ggttctgtct
     5281 ggcgtactca gaggctagtt gaaaggtaag ataacttttt cctacaccac ctttgttatt
     5341 ccatacggca tagatcgcca tctattactc ctaatctagt tttgttgtta gcttaacgtc
     5401 agagtactag agattattgt tctaatgtta gtttgtttca gctaactgag tgtgtttcga
     5461 gcagtagacc ctggttaggg gatacgtatt ggcatgactt ctttggcata gtaggaacgt
     5521 gat
//
