LOCUS       NZ_CP027065            10060 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain WCHKP8F4 plasmid p1_095084, complete
            sequence.
ACCESSION   NZ_CP027065
VERSION     NZ_CP027065.3
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06671960
            Assembly: GCF_002258145.4
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Yao,Z., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain WCHKP095084
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Yao,Z., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 10060)
  AUTHORS   Yao,Z., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   4  (bases 1 to 10060)
  AUTHORS   Yao,Z., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 09:02:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,388
            Genes (coding)                    :: 5,262
            CDSs (with protein)               :: 5,262
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 47 of 126
            Pseudo Genes (incomplete)         :: 82 of 126
            Pseudo Genes (internal stop)      :: 11 of 126
            Pseudo Genes (multiple problems)  :: 14 of 126
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP027065.3.
            On Aug 18, 2021 this sequence version replaced NZ_CP027065.2.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP8F4"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p1_095084"
                     /geo_loc_name="China: Sichuan, Chengdu"
                     /collection_date="2015-04"
     gene            join(10016..10060,1..105)
                     /locus_tag="B7D38_RS00005"
                     /old_locus_tag="B7D38_00015"
     CDS             join(10016..10060,1..105)
                     /locus_tag="B7D38_RS00005"
                     /old_locus_tag="B7D38_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            1589..3553
                     /locus_tag="B7D38_RS00010"
                     /old_locus_tag="B7D38_00005"
     CDS             1589..3553
                     /locus_tag="B7D38_RS00010"
                     /old_locus_tag="B7D38_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            3553..4284
                     /gene="mobC"
                     /locus_tag="B7D38_RS00015"
                     /old_locus_tag="B7D38_00070"
     CDS             3553..4284
                     /gene="mobC"
                     /locus_tag="B7D38_RS00015"
                     /old_locus_tag="B7D38_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            4291..4821
                     /locus_tag="B7D38_RS00020"
                     /old_locus_tag="B7D38_00065"
     CDS             4291..4821
                     /locus_tag="B7D38_RS00020"
                     /old_locus_tag="B7D38_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4848..5027)
                     /locus_tag="B7D38_RS00025"
                     /old_locus_tag="B7D38_00060"
     CDS             complement(4848..5027)
                     /locus_tag="B7D38_RS00025"
                     /old_locus_tag="B7D38_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5053..5481)
                     /locus_tag="B7D38_RS00030"
                     /old_locus_tag="B7D38_00055"
     CDS             complement(5053..5481)
                     /locus_tag="B7D38_RS00030"
                     /old_locus_tag="B7D38_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(5645..5959)
                     /locus_tag="B7D38_RS00035"
                     /old_locus_tag="B7D38_00050"
     CDS             complement(5645..5959)
                     /locus_tag="B7D38_RS00035"
                     /old_locus_tag="B7D38_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5947..6225)
                     /locus_tag="B7D38_RS00040"
                     /old_locus_tag="B7D38_00045"
     CDS             complement(5947..6225)
                     /locus_tag="B7D38_RS00040"
                     /old_locus_tag="B7D38_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(6400..6765)
                     /locus_tag="B7D38_RS27370"
                     /old_locus_tag="B7D38_28425"
     CDS             complement(6400..6765)
                     /locus_tag="B7D38_RS27370"
                     /old_locus_tag="B7D38_28425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(6762..7133)
                     /gene="tolA"
                     /locus_tag="B7D38_RS00050"
                     /old_locus_tag="B7D38_00035"
     CDS             complement(6762..7133)
                     /gene="tolA"
                     /locus_tag="B7D38_RS00050"
                     /old_locus_tag="B7D38_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(7407..7652)
                     /locus_tag="B7D38_RS00055"
                     /old_locus_tag="B7D38_00030"
     CDS             complement(7407..7652)
                     /locus_tag="B7D38_RS00055"
                     /old_locus_tag="B7D38_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7979..9664
                     /locus_tag="B7D38_RS00060"
                     /old_locus_tag="B7D38_00025"
     CDS             7979..9664
                     /locus_tag="B7D38_RS00060"
                     /old_locus_tag="B7D38_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            9674..9931
                     /locus_tag="B7D38_RS00065"
                     /old_locus_tag="B7D38_00020"
     CDS             9674..9931
                     /locus_tag="B7D38_RS00065"
                     /old_locus_tag="B7D38_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     BGC             2979..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(209..787)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3554..4281
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 ggctttcttc tggccgcatg tcaggcaaat tatatccggg atgttcaggg cggaacggtg
       61 gcaccatcgt cctcctctga actgacgggg atcgcggttc agtagaaaag atcaaaggat
      121 cttcttgaga tccttttttt ctgcgcgtaa tcttttgccc tgtaaacgaa aaaaccacct
      181 ggggaggtgg tttgatcgaa ggttaagtca gttggggaac tgcttaaccg tggtaactgg
      241 ctttcgcaga gcacagcaac caaatctgtc cttccagtgt agccggactt tggcgcacac
      301 ttcaagagca accgcgtgtt tagctaaaca aatcctctgc gaactcccag ttaccaatgg
      361 ctgctgccag tggcgttttg cgtgctttcc cgggttggac tcaagtgaac agttaccgga
      421 taaggcgcag cagtcgggct gaacggggag ttcttgctta cagcccagct tggagcgaac
      481 gacctacacc gagccgagat accagtgtgt gagctatgag aaagcgccac acttcccgca
      541 agggagaacg gcggacaggt atccggtaaa cggcagggtc ggaacaggag agcgcaagag
      601 ggagcgacct accggaaacg gtggggatct ttaagtcctg tcgggtttcg cccgtactgt
      661 cagattcatg gttgagcctc acggctccca cagatgcacc gaaaaagcgt ctgtttatgt
      721 gaactctggc aggagggcgg agcctatgga aaaacgccac cggcgcggcc ctgccgtttt
      781 gcctcacatg ttagtcccct gcttatccac ggaatctgtg ggtaactttg tatgtgtccg
      841 cagcgcccgc cgcagtctca cgcccggagc gtagcgaccg agtgagcgag gaggcgcaat
      901 ttccccggca cctgcaagac ggtcaccgga cgcgctgatg tgggatattg tcccctgatt
      961 aagtctttct attccctgac gatcaattat caggataggt gaaccatgtc ggtttttcgc
     1021 tgaacatttt acgttaacct atctctgttg cttttttctc cttgcggaga aatgcaagtc
     1081 atccccgctg gggcgtggct gacttcaggg gaaaagacct tgcttacgct ggctggacgc
     1141 caccgacgca cggtcttttc ccctctttct ggctctgtcc ggctggacgc cggccacgcc
     1201 agaaattcac ccggcccccg tcaggctcac gcctgaccac ccacaaaaat ccacggtgct
     1261 ggacgcacct gacgaagcag caaagctgct cctgattttg cgggtggtca gccgctcgct
     1321 atggcgaact ctgacggggg ccggaacacc ggaaaagacc gcactggggt gccgcctttt
     1381 cctctcacac gctggggcgt gctcgttgtt cctccggggc tgcgcctttt ccgcatgggg
     1441 cggaaagagg ctggcctccg cggtgaaagc tgaacgcaat caccacggac gtgaccgccg
     1501 ccgccatgcc ttggggcatg tccgcggctt gtgcagcggg ggctgcgcat cacgccggta
     1561 aacccgtcgt ctgaaggaga gagattgcat gtttaatacg gattcgcttg cctggcagtg
     1621 gcatatcccg gagctggccc ggcagattta cccgtgggtc agctggctgt accgggagta
     1681 ttacagtctg gccggttttt ttctggttat cggcctgttt ttcggtctgg ctgaactctg
     1741 ggccggccga atgcgttttg tggggctgag ccataaggcg cggcgggagt atgccgatcg
     1801 ctataagcgc gataataacc ggactgtgcc attttcggtg ctggcctcgc tggattcacc
     1861 cgggcggatg gtcagggcac ggcgacatgt ctggaactac gcgtactggc tgaccggcgc
     1921 tgccggtctg gcccttctgg gggggctggt tgtcgccgtg tttatcgaga gctggggaga
     1981 aaccgtcctg cgcccggacc tctatgcgaa cgcagattat tacctgctgc agccggctaa
     2041 cctgttcggg atagcctggt gtgccggagg gcttgtcgcc ggcgtccttc tggcgcggct
     2101 gctggcgttc ctgctgcttg atggtcattt tgcggccgct gacgaggcgg tgaacgcgaa
     2161 actgaatcag gagagccggc gcagcagcca gcggaccgga gaaatgacag acgtccgcca
     2221 cctgcatttc ggggagccgg taccggttaa cgcgctggcc gatttttcca cggagcaggc
     2281 ccggaaacag caggcggtgt ttctgggtaa ggatgagcag ggccagccgg tgctggtccc
     2341 tcgtgatacc tggcgcaaga ccaatattca gatcctcggc ctgcccggga gcggcaagag
     2401 cgcgatggcc accaacgcgc tcattcgctg cgtacgcgat ttcggggacg ctgtggtgta
     2461 cttcgacccc aaaggggacg cgtgggcgcc gcacgtcttc cgggcgcact gcccggactt
     2521 caccctgctg gatctgcggc cggggaaacc ggcgcagctg aatctgttcc gcgatctcga
     2581 ccagtacgcc ctgaagaatc tgctggttgc cgggtttaac ctgagtgaga ccggcgacgt
     2641 ggcggaccac taccgcatca gcgagcagaa agcggcgaag ctgattgcgg agcagttccc
     2701 gcagggggca aacattcagc aggtgctggc ggcggcgtac gcgttgccgg aggccctgaa
     2761 aaaggacgtg aaggggctga tcaccaagct ggagaacgtg gccgacctga gcgtcctgca
     2821 gacggacagc ggtatcgatg tggcggggat aatcaacggc ggcggctgcc tgtacgtcat
     2881 cggctcgatg gacgatgagg cggtgatccg ggtgcagaag atgctgtttg cccgctgcgc
     2941 gcaaatcatc attgcgcggg atgagttccg gaggtggccg cacgcgagca tcatgctcga
     3001 tgagattaaa tatctgctgt cgaagtacgt gctgaacgcg ctggggacgc tgcgcagccg
     3061 ggactgcaac ctgctgctcg cgcaccagtc gctgggcgac ttcggtcagt gcgggcagga
     3121 tctgcccgct gattttgtga aaaccacggt actggataac acgccgatcc gctggttcta
     3181 tcgcgcggcc agccaggagt ccgcgcagtg ggcggcgggc cagaccgggg agatccgggt
     3241 ggacgtggag cgccgccggg ccagccggga ggcggggaac gtggagcaca tcagcggcga
     3301 cagcttcatc cagaaggagg cgcggccgct gtttgacgtg aacaccctgc aacacctgcc
     3361 tgacgggttc gcggtgatga ccgggctggg cgtggcgcgg ctggcgttca gcagcccgct
     3421 gcgggttgag cgccgggaaa tttcgctgaa aaccttcccg gtgctggcga aaacggaccc
     3481 gctggcggag tacctgcctc cggccgacgg cggcggacca aaagcggatg atgattttgc
     3541 ggggctttac tgatgacact ggagcagtac aacgtcagcc acgcgaagcg gcaggcgcgg
     3601 aacgcggaga aaacacggct gaccctgcgc tggctgcggg aagagctgtg ttccacggcg
     3661 gagctggttg cgcgacggct ggggattgcg gcggtacagc cggtttaccg cttcctggac
     3721 agtctggtgg cgaaagggct gctggtccgg gcgaaatacc cggttgacgg ccgtcaggtc
     3781 agcgtgtggg ggctgacgcc gcacggggtg gcgttctcgt tcgacgagga cgagccgctg
     3841 acggacgtta ttccgtttca gccgtcaagg gtgtcggcgg cgcagctgcc gcaccggctg
     3901 gccgtgcagt ccctgcgcct ctccatggag ggccgggggg ccaccggctg gcggtacctg
     3961 caccgtatgg ccctgaaagg gatgaaagtg ccggacgcgc tggcggagct ggacggcaga
     4021 acggtggcat ttgaggtaga gcgcaccgtc aagagccgca ggcggtatca ggaagtggtg
     4081 tccgggtatc tgttcaaccg ccgggcgaac ggcatcgatg aaatctggta catctgccct
     4141 gaccggccga cgcttgtccg ggtacagcgg gcgattctgg cggtggatga aatcgtgaat
     4201 ccacagaccg gcgaggcccg caaaacggtg gagctggacc gggagaggct gttcgcgtgc
     4261 tttaaattta tgacaacgga gtgagggatt atggcgctgt acatggtatt tattgaggac
     4321 gtggaagaca aagacggcga gcgctgcatt atgccaacca gcgagcatct ggaagacatg
     4381 gtggtgctcg cctggcagac cggggcgtat gttttctgtt ttgtcgaccg ggcccagcat
     4441 ctggcgatga tgaaatccat cgggatgtat gacgagcggc tcaacagcga cgaaaaagcc
     4501 acgtactttt tcaccggtga aaatccgcaa tgggaacggg acaaacccac aggaatgaag
     4561 ctgtgtgatt acctctatcg aaaggtggag cggttcgcca tcgagaacgg tgtgcaggtg
     4621 gttgatgccg ggaagtgctc accggagcag attaacgcga tgattgaggt ggtcagcgag
     4681 cggcacgggg aaagcgcggt gaacgagaag ctgaaagcgg agctgatgcg ggaatataac
     4741 agccgctatt ttaacgtgcc ggtgcctgaa ctgccggagg acaaacccgc ccgccggtgg
     4801 tggccgttcg gcaaaaaata acgccagcac cggcatggcc ggtgctgtta ttcttccccg
     4861 agcttatccc gcatcacctg ccagagtgtt tccgcatcct cgtgaagccg ttcgcacatg
     4921 tcggccgggg tatccatgtc cagctcgttc agcttttcca gcaacagcag cgactgatcc
     4981 ttaatgaatt tagccatatt gagggcagtt ttttcctgct tcgtcatgtt ctccattccc
     5041 ttcggtaagt tctcagctaa accagccgga ccagaagcgt tttttcttct gtgtcagttc
     5101 tgttgtcaat ctgtccactt cggcctggag tttccggcgc tcatcatcgt gctggcggcg
     5161 ttcttcttcc cgggatgttt tatcttccag catcagggtg acagcctgcc ggagatcggc
     5221 cagttccttt tgcattgcca gaagctgttc ctgtgggaaa tttttttgtg tattttcacg
     5281 gtgtgaaatg ccgggaattt gtttctcagg gatattcaca tttccgtata cacggatcag
     5341 ttctgacaca tccacgacag ggttgttttt cccgtcacgt gacacggtaa cctttccctg
     5401 ttttacatga ttgtatagcg ttcttctggt aatgcccgcg gcttgtgctg cctgtgaaag
     5461 gttgagcaat gttttcgcca ctgtataccc ctgattttat cgggtgtgaa aggtgtgtaa
     5521 tttctcacgg taaatgtatc gctgaatttt acccgttttt ccgttcagga tgtggttttc
     5581 tgacgcgctg cgcttgtcaa accggcgacg gccacgcaat gaattgcgcc cccgtcatcg
     5641 gttattagag gctgtctgcc cccggatacc cggattcttt ctgatgcctg acggtgacga
     5701 tcacaatctg gtccatttcc cggtcgtggc ggtagagcat cacatagcca ctgtcgccaa
     5761 acccgatcac cagttcctta tattccagcg gcagaaacgg caccgggcgg ccaatgtctg
     5821 gcaatgtttt cagttgctgg atagcccgga cgatcacctc acctcctttt ctggctgcca
     5881 gccgattttt ggttttgaga aagtcctgaa ggcgttgtaa atcgcgctgt gccagcgcgg
     5941 aaatcgttac ctgtggcatg gcggagcatc ctgctcgttc tcagttcccc aggtacttat
     6001 ccaggcttcc gcttcttccg cagtaaggtg cagacccgtt tcctgatagt gctgccatgc
     6061 ggcctgtccg tcccgcaggt actggctgcg cttctcttcc cggtcgatgt attcggtgat
     6121 tgcctccagc attagcgcgt gcgctgagcg gtgccggtca tcggccagcg ttttcagccg
     6181 gtccttaagt tcttcatcga gcctgatgga tgttgctgat gacataaaag cccctttgta
     6241 gtcatatgta ttacacatga ctactttaat ccgtggcggc gctatttgcc agaaatacgg
     6301 cttttgctgc ccatttttca gcttcgcttc cactaatcag ggctgctaca ggctcactca
     6361 tcatccagga ctattgcagc ctgcgggtta aaggacctgt tactggcggc aaccatcatc
     6421 attgagcttt tgggtggtga ctggtcgcgg atcctgtaag ccgtcatttt tcagactgaa
     6481 ttcaaaattt ataacctgtc gcattgccgg gtgccctgcg ggatatagcc agtgcctcat
     6541 tgccgtcagc gtttcccgaa aaaaaaggtg acagccggta agcgacacgt tgtccactcg
     6601 tccatcgtcc agaatatcaa acgtggcggt cacctgacct tcgatgcgtt ttgctgcggc
     6661 ctgtaccggg taggttggct gcagtgtggt tacgggtttt agtggtatcg catgtaccgg
     6721 agtgaggaac atggccgcca gcactgtcag agtaaaagct ttcatggtct gaaatccaga
     6781 ggggcgtttt tgaatatttg ccaggtattt tcatccggtg ccggcggaat tttggcgtgg
     6841 gtaacggctg atattgctgc tttacagagt ttcggatctc ctccttcagc aatggcgctg
     6901 ttcagagtgc catcacgttg tatggatatc cgcacggtgc acatttttcc cgcataggcc
     6961 tctggttcaa acatctgcat ttcaacagcg gtcttgatgg ccgcagcgta gctactgatt
     7021 ttagatgact tatccagagt ataaattttc ggcggctggg tggtggaatg cttacaggcc
     7081 gtcaggagta aggggctgca caatgccagc aggataaagg gtattaaacg catcgggttg
     7141 atgacctgtc agtaaaaaat gttacatgta tatttgtcac tctttacggc cacgttcatt
     7201 tacataaaca cggcttatcg tatcgttttt acaccaaacg gcccgccatg cagttgcgtg
     7261 cataggcatg cagcgggtta caatttaccg ggcgcgtttc cggcggtttt ccgggtggtt
     7321 tgttgcctgt tttatcccgt agccgccgga aacgccctca gaccgtctga gcggtgcggg
     7381 caatgcgacg ttatggtaaa taccccttac cggcggcgct gtggctgcca ggtgaaggtg
     7441 gtttccgtag cctcgtattt ctcccgacgc atttcctgcg cccgcctggc ttcacgttcg
     7501 tcctgaatgg cacgaagcac cctttcccgc acctgttgtt ttaccgcctt tggcagcgtt
     7561 ttgttccctt tcaggcccat ggccagtgcc tgagcgacaa aggcgtccgc caggcgcttt
     7621 tcttcagcgg gagtcagttt caggtgcgtc atagtggcct ccgggcgtct caggtttccc
     7681 gaacagttta accgcaggtc ctctcctcat gccagttagc tttcagatgt gtctatacca
     7741 tctgaaatgt aagtgtgatg aatgtcttaa ataataaaat ttgttgccat gagaaatttt
     7801 cgggaaaaga cgcgatctgc ttacaggtgg ctccgggcgc ttttttgtgc ctgaagatga
     7861 gaactaatct catatattga cacctgaaaa cgggaggagt aaggtaataa ttatactgtg
     7921 tatatgtaca gtatgttggc tttaatttat tgttttaaat gtcaaagagg aaaacggtat
     7981 gagtggtgga gacggtcgag gtccgggtaa ttcaggtctg ggacataatg gtggtcaggc
     8041 cagtgggaat gtgaacggta cgtctggtaa aggtggccct tcatcaggtg gtggtacgga
     8101 tccaaacagc gggccgggct ggggtacgac gcatacgccg aatggagata ttcataacta
     8161 taatccgggg gagtttggta acggcgggag taaacccggt ggtaatggtg gtaacagcgg
     8221 caatcatagc ggtagctctg gtggcggaca gtcttcggcc actgcgatgg ccttcggtct
     8281 gcctgccctg gctactccgg gggctgaagg actggcttta tcagtttccg gcgatgcgtt
     8341 gtcggccgcc gttgctgatg tgctggctgc cctgaagggg ccgtttaagt ttggtctgtg
     8401 ggggattgcg atctacggtg tgctgccttc tgagattgca aaagatgatc cgaaaatgat
     8461 gtcaaaaatt atgacgtcat taccggccga tgcggtgacg gagactccgg caagtacttt
     8521 accactggac caggcgacgg ttcgtgtcag acaacgggtt gtggatgtgg tgaaggatga
     8581 gcggcagcat attgcggttg tcgcaggtcg gccaatgagt gtccctgtgg tggatgcgaa
     8641 accgacaaaa cgtccggggg tattcagtgt gtcgattccg ggtctcccgt ctctgcaggt
     8701 gagcgtaccg aaaggtgttc cgacagcgaa agccccgcca aaaggcattg ttgctgaaaa
     8761 aggtgattca cgtccggctg gttttacagc cggtggtaac tcccgtgagg ccgttattcg
     8821 tttcccgaaa gagaccggac agaagccggt ttatgtgtcg gtgacagatg ttcttacccc
     8881 ggcacaggta aaacagcgtc aggaggaaga aaagcgtcgc cagcaggcat gggacgccgc
     8941 tcatccggaa gaggggctga aaagagacta tgataaagcg aaagctgagc tggatgccga
     9001 agataaaaat attgcgacct taaacagccg cattgcatcg acagagaagg cgctccccgg
     9061 tgcaagggct gctgttcagg aagccgataa aaaggtaaaa gaggcagagg caaataaaga
     9121 tgattttgtg acttataacc cgcctcatga atatggctcc gggtggcagg atcaggttcg
     9181 ctatcttgat aaggatattc agaatcagaa tgagaaatta aaagcggctc aggcatcttt
     9241 aaacgcaatg aatgaatcct tatccagaga taaggctgca cttaccgggg cgatggagag
     9301 ccggaaacaa aaggagaaaa aagcgaagga tgcagaaaat aagttaaatg aggaaaagaa
     9361 caagcctcgc aaggggacta aagactacgg ccatgattat catccagtac ctaaaacaga
     9421 agacataaag ggacttggag agttaaagcg aggtgatcct aaaacaccta aacaaggtgg
     9481 tggtggtaag cgggcccgat ggtatggcga taaaaaacgt aaaatttatg aatgggattc
     9541 ccagcacggt gagcttgaag ggtatcgtgc cagtgatggc gaacacctag gcgcatttga
     9601 tcccaaaacg ggtaagcagg ttaaagggcc agatccaaaa cggaacatta aaaaatatct
     9661 ttgagaggta aatatggggc ttaaattacg tttgacatgg tttgataaga aaactgaaga
     9721 gtttaaaggg gaagagtatt ctaaagactt tggtgacgat ggttctgtca ttgaaagctt
     9781 gggaatgcct ttaaaggata atattaataa tggtgagttt gatttgaaga aagaatgggt
     9841 tcctttacta cagccatttt ttaaaaatca aataaaacct gatgtggata attgttatat
     9901 agcttttgat tatcgggacg gtaactggta acaaaaaatt atcaaggatg atttgaaata
     9961 ctgacttccg gtgttcaggg atgaatgccg gagtctttaa atacagggaa agtatatgaa
    10021 aaaggtaaag actatttttc tttttatatt gattgttgct
//
