LOCUS       NZ_CP027058            13841 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 2_GR_12 plasmid ColRNAI, complete
            sequence.
ACCESSION   NZ_CP027058
VERSION     NZ_CP027058.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04868722
            Assembly: GCF_001702045.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 13841)
  AUTHORS   Pitt,M.E., Nguyen,S.H., Duarte,T.P.S., Blaskovich,M.A.T.,
            Cooper,M.A. and Coin,L.J.M.
  TITLE     Evaluating the Resistome of Extensively Drug-Resistant Klebsiella
            pneumoniae via MinION Direct RNA Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13841)
  AUTHORS   Pitt,M.E., Nguyen,S.H., Duarte,T.P.S., Blaskovich,M.A.T.,
            Cooper,M.A. and Coin,L.J.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2018) Institute for Molecular Bioscience, The
            University of Queensland, Carmody Road, Brisbane, Queensland 4067,
            Australia
REFERENCE   3  (bases 1 to 13841)
  AUTHORS   Pitt,M.E., Elliotta,A.G., Cao,M.D., Ganesamoorthy,D., Karaiskos,I.,
            Giamarellou,H., Abboud,C.S., Cooper,M.A. and Coin,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2016) Institute for Molecular Biosience, The
            University of Queensland, 306 Camordy Rd, St Lucia, Brisbane,
            Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-DEC-2017
            Assembly Method                   :: Unicycler v. 0.3.1
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 67x
            Sequencing Technology             :: Illumina MiSeq/ ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 02:52:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,048
            CDSs (total)                      :: 5,921
            Genes (coding)                    :: 5,745
            CDSs (with protein)               :: 5,745
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 81 of 176
            Pseudo Genes (incomplete)         :: 100 of 176
            Pseudo Genes (internal stop)      :: 16 of 176
            Pseudo Genes (multiple problems)  :: 18 of 176
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP027058.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13841
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2_GR_12"
                     /isolation_source="Urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="ColRNAI"
                     /geo_loc_name="Greece: Athens"
                     /lat_lon="38.03 N 23.79 E"
                     /collection_date="2012-03-12"
                     /collected_by="Hygeia General Hospital"
                     /genotype="ST258"
     gene            complement(join(13656..13841,1..372))
                     /locus_tag="A6D68_RS30820"
                     /old_locus_tag="A6D68_031035"
     CDS             complement(join(13656..13841,1..372))
                     /locus_tag="A6D68_RS30820"
                     /old_locus_tag="A6D68_031035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            536..>3508
                     /locus_tag="A6D68_RS30825"
                     /old_locus_tag="A6D68_031040"
                     /pseudo=""
     CDS             536..>3508
                     /locus_tag="A6D68_RS30825"
                     /old_locus_tag="A6D68_031040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /name="Tn3-like element Tn3 family transposase"
     gene            <3576..3866
                     /locus_tag="A6D68_RS30830"
                     /old_locus_tag="A6D68_031045"
                     /pseudo=""
     CDS             <3576..3866
                     /locus_tag="A6D68_RS30830"
                     /old_locus_tag="A6D68_031045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /name="Exc2 family lipoprotein"
     gene            4172..4435
                     /locus_tag="A6D68_RS30835"
                     /old_locus_tag="A6D68_031050"
     CDS             4172..4435
                     /locus_tag="A6D68_RS30835"
                     /old_locus_tag="A6D68_031050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            4425..4724
                     /locus_tag="A6D68_RS30840"
                     /old_locus_tag="A6D68_031055"
     CDS             4425..4724
                     /locus_tag="A6D68_RS30840"
                     /old_locus_tag="A6D68_031055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4786..5163)
                     /locus_tag="A6D68_RS30845"
                     /old_locus_tag="A6D68_031060"
     CDS             complement(4786..5163)
                     /locus_tag="A6D68_RS30845"
                     /old_locus_tag="A6D68_031060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            5232..5411
                     /locus_tag="A6D68_RS30850"
                     /old_locus_tag="A6D68_031065"
     CDS             5232..5411
                     /locus_tag="A6D68_RS30850"
                     /old_locus_tag="A6D68_031065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5439..5969)
                     /locus_tag="A6D68_RS30855"
                     /old_locus_tag="A6D68_031070"
     CDS             complement(5439..5969)
                     /locus_tag="A6D68_RS30855"
                     /old_locus_tag="A6D68_031070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5976..6707)
                     /locus_tag="A6D68_RS30860"
                     /old_locus_tag="A6D68_031075"
     CDS             complement(5976..6707)
                     /locus_tag="A6D68_RS30860"
                     /old_locus_tag="A6D68_031075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(6707..8671)
                     /locus_tag="A6D68_RS30865"
                     /old_locus_tag="A6D68_031080"
     CDS             complement(6707..8671)
                     /locus_tag="A6D68_RS30865"
                     /old_locus_tag="A6D68_031080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(10152..10301)
                     /locus_tag="A6D68_RS30875"
                     /old_locus_tag="A6D68_031090"
     CDS             complement(10152..10301)
                     /locus_tag="A6D68_RS30875"
                     /old_locus_tag="A6D68_031090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(10386..10643)
                     /locus_tag="A6D68_RS30880"
                     /old_locus_tag="A6D68_031095"
     CDS             complement(10386..10643)
                     /locus_tag="A6D68_RS30880"
                     /old_locus_tag="A6D68_031095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(10653..11474)
                     /locus_tag="A6D68_RS30885"
                     /old_locus_tag="A6D68_031100"
     CDS             complement(10653..11474)
                     /locus_tag="A6D68_RS30885"
                     /old_locus_tag="A6D68_031100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199321.1"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]; GO:0043022 - ribosome binding
                     [Evidence IEA]; GO:0003723 - RNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /protein_id="WP_004199321.1"
                     /translation="MSFRSTERQTPTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDK
                     AKAELDAEDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEY
                     GSGWQDQVRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKD
                     AENKLNEEKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYG
                     DKKRKIYEWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            complement(11590..>11760)
                     /locus_tag="A6D68_RS30890"
                     /old_locus_tag="A6D68_031105"
                     /pseudo=""
     CDS             complement(11590..>11760)
                     /locus_tag="A6D68_RS30890"
                     /old_locus_tag="A6D68_031105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /name="serine hydrolase"
     gene            complement(11815..12517)
                     /locus_tag="A6D68_RS30895"
                     /old_locus_tag="A6D68_031110"
                     /pseudo=""
     CDS             complement(11815..12517)
                     /locus_tag="A6D68_RS30895"
                     /old_locus_tag="A6D68_031110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042893601.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(<12583..12798)
                     /locus_tag="A6D68_RS30900"
                     /old_locus_tag="A6D68_031115"
                     /pseudo=""
     CDS             complement(<12583..12798)
                     /locus_tag="A6D68_RS30900"
                     /old_locus_tag="A6D68_031115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /GO_function="GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing protein"
                     /name="nucleotidyltransferase domain-containing protein"
     gene            complement(12868..13422)
                     /gene="aac(6')-Ib"
                     /locus_tag="A6D68_RS30905"
                     /old_locus_tag="A6D68_031120"
     ARG             complement(12872..13422)
                     /name="aac(6')-Ib"
                     /gene="aac(6')-Ib"
                     /gene_name="AAC(6')-Ib family aminoglycoside
                     6'-N-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(12530..12786)
                     /name="aadA15"
                     /gene="aadA15"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.14"
                     /coverage="32.32"
     ARG             complement(12869..13473)
                     /name="AAC(6')-Ib10"
                     /gene="AAC(6')-Ib10"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="98.97"
                     /coverage="99.01"
     Typing          9471..10049
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5980..6707)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.34e-150"
ORIGIN
        1 ttctaggatc ctccggcgtt cagcctgtgc cacagccgac aggatggtga ccaccatttg
       61 ccccatatca ccgtcggtac tgatcccgtc atcaatgaac cggactgcca cgccctgagc
      121 gtcaaattcc tttatcagtt ggatcatatc ggcagtgtcg cggccaagac ggtcgagctt
      181 cttaaccaga atgacatcac cttcctccac cttcatcctc agcaaatcca gcccttcccg
      241 gtctgttgaa ctgccggatg ccttatcggt aaatatacgg tttgctttca cacctgcgtc
      301 tttgagtgct ctgacctgaa gatcaagaga ctgctgactg gttgagaccc gagcgtaacc
      361 aaaaagtcgc ataaaaatgt accttaaatc gaatatcgga caactcatgt ctattattac
      421 aaatttacga tttaatagac atattaatgt aacagtttta cgatgtccga taatttataa
      481 catttcgtac ggttggaaaa atgttactaa atgcccgtca ggcagggagg ccgatatgcc
      541 cgttgacttt ctgaccactg agcagactga aagctatggc agattcaccg gtgaaccgga
      601 tgagcttcag ctggcacgat attttcacct tgatgaagca gacaaggaat ttatcggaaa
      661 aagcagaggt gatcacaacc gtctgggcat tgccctgcaa attggatgtg tccgttttct
      721 gggcaccttc ctcaccgata tgaatcatat tccttccggc gtccggcatt ttaccgccag
      781 acagctcggg attcgtgata tcaccgttct tgcagaatac ggtcagaggg aaaatacccg
      841 ccgtgagcat gcagcgctga tacgtcagca ctatcagtat cgtgaatttg cctggccctg
      901 gacatttcgc cttacccgtc ttttatatac ccggagctgg ataagcaacg aacgtcctgg
      961 cctgcttttc gacctggcga cagggtggct tatgcaacat cgtattattc tccccggagc
     1021 caccacgctg acccggttga tttcagaggt aagggaaaag gcgacgttgc gcctgtggaa
     1081 caaactggca ctgataccgt cagccgaaca gcgttcacag ctggagatgc tgctggggcc
     1141 aactgattgc agccgcctgt ctttactgga atcactgaaa aaaggccctg tgaccatcag
     1201 tggtccggcg tttaatgaag caattgaacg ctggaaaact ctgaacgatt ttggcctgca
     1261 tgctgaaaac ctgagtacac tcccggctgt gcgcctgaaa aatctcgcac gttatgctgg
     1321 tatgacttcg gtgttcaata ttgccaggat gtcaccgcag aaaaggatgg cggttctggt
     1381 tgcctttgtc cttgcatggg aaacgctggc gctggatgat gcactggacg ttctggacgc
     1441 catgctggcc gttatcatcc gtgacgccag aaagattggg cagaaaaaac ggctccgctc
     1501 gctgaaggat ctggataaat ctgcattggc gctcgccagc gcatgttcgt acttgctgaa
     1561 agaagaaaca ccggacgaat cgattcgtgc tgaggtgttc agctacatcc ctaggcaaaa
     1621 gctggctgaa atcatcacgc ttgtccgtga aattgcccgg ccctcagacg ataattttca
     1681 tgacgaaatg gtggagcagt acgggcgcgt tcgtcgtttc ctgccccatc tgctgaatac
     1741 cgttaaattt tcatccgcac ctgccggggt taccactctg aatgcctgtg actacctcag
     1801 ccgggagttc agctcacggc ggcagttttt tgacgacgca ccaacggaaa tcatcagtca
     1861 gtcatggaaa cggctggtga ttaacaagga aaaacatatc acccgaaggg gatacacgct
     1921 ctgctttctc agtaaactgc aggatagtct gagacggagg gatgtctacg ttaccggcag
     1981 taaccggtgg ggagatcctc gtgcaagatt actacagggt gctgactggc aggcaaatcg
     2041 gattaaggtt tatcgttctt tggggcaccc gacagacccg caggaagcaa taaaatctct
     2101 gggccatcag cttgatagtc gttacagaca ggttgctgca cgtcttggcg aaaatgaggc
     2161 tgtcgaactc gatgtttctg gcccgaagcc ccggttgaca atttctcccc tcgccagtct
     2221 tgatgagccg gacagtctga aacgactgag caaaatgatc agtgatctgc tccctccggt
     2281 ggatttaacg gagttgctgc tcgaaattaa cgcccatacc ggatttgctg atgagttttt
     2341 ccatgccagt gaagccagtg ccagagttga tgatctgccc gtcagcatca gcgccgtgct
     2401 gatggctgaa gcctgcaata tcggtctgga accactgatc agatcaaatg ttcctgcact
     2461 gacccgacac cggcctgaac tggacaaaag cgaactatct gcgggctgaa actatcacca
     2521 gcgctaatgc cagactggtt gattttcagg caacgctgcc actggcacag atatggggtg
     2581 gaggagaagt ggcatctgca gatggaatgc gctttgttac gccagtcaga acaatcaatg
     2641 ccggaccgaa ccgcaaatac tttggtaata acagagggat cacctggtac aactttgtgt
     2701 ccgatcagta ttccggcttt catggcatcg ttataccggg gacgctgagg gactctatct
     2761 ttgtgctgga aggccttctg gaacaggaga ccgggctgaa tccaaccgaa attatgaccg
     2821 atacggcagg tgccagcgat cttgtctttg gccttttctg gctgctggga taccagtttt
     2881 ctccacgcct ggctgatgcc ggtgcttcgg ttttctggcg aatggaccat gatgccgact
     2941 atggcgtgct gaatgatatt gccagagggc aatcagatcc ccgaaaaata tccttcagtg
     3001 ggacgaaatg atccggaccg caggctccct gaagctgggc aaagtacagg cctcagtgct
     3061 ggtccgttca ttgctgaaaa gtgaacgtcc ctccggactg actcaggcaa tcattgaagt
     3121 ggggcgcatc aacaaaacgc tgtatctgct taattatatt gatgatgaag attaccgccg
     3181 gcgcattctg acccagctta atcggggaga aagccgtcat gcagttgcca gagccatctg
     3241 tcacggtcaa aaaggtgaga taagaaaacg atataccgac ggtcaggaag atcagttggg
     3301 agctctgggg ctggtcacta acgccgtcgt gttatggaac actatttata tgcaggcagc
     3361 tctggatcat ctccgggcgc agggtgaaac actgaatgat gaagatatcg cacgcctctc
     3421 cccgctttgc cacggacata tcaatatgct cggccattat tccttcacgc tggcagaact
     3481 ggtgaccaaa ggtcatctga gaccattaaa agaggcgtca gaggcagaaa acgttgctta
     3541 acgtgagttt tcgttccact gagcgtcaga ccccttttga tcccaatttt tccacccaga
     3601 aagctgacac aacacgaatg atggtgcctt ttttccggca gttctgggat atgggcgcaa
     3661 aggataaagc gaccggaaaa tcacggagtg atgtgcagca acgcattcag cagtttcaca
     3721 gtcaggaatt tttaaactca ctccaaggca caacacaatt tgcgggtacc gattaccgca
     3781 gcaaagacct cacaccgaaa aaatcaaggc tactggctga caccatttca gctgtttatc
     3841 tcgatggcta tgaaggcaga cagtaagtgg atttaccata acgcgtcatt acgcgcaccg
     3901 ctcagacggg ctcagggcgt ttccggcggc tacgggataa aacaggcaac aacccccccg
     3961 gaaaactgcc ggaaacgcgc ccgataaatt ttatcctaat gcatgcctat gcacgcaact
     4021 gcatggcata tgttttgacg taaaatggat gccgtaagcc cttttttacc gactttctgc
     4081 ttcatcagcc tgactgacac gaccatattc tttcagaccg ccccctgcta aacttccttc
     4141 atattgccgc attaacccgg gaggctccgc aatgagcaac actacacact acgaaaacgc
     4201 caactttctt cgggaactgg ctgaaagcct gccccgcata ctgccggaag gtggcccgga
     4261 caaagctgcg ctgttgcagc gactggctaa cgaagaactg gcccaggcag agtatgaaga
     4321 tcaggttcgc gccaaagtga ccgccgcccg tgctgacacg cgtccgggta tgaccacgga
     4381 acaactccgc cagcgcctgc atggccgtta tcaggaactg cgcgatgccg tatgacgtgg
     4441 aatggaagca gggagccatc gaggatgtaa cgacgctgtt tgactatatc gcggaaaatt
     4501 catccctgtg ggatgcgcag aacgttaccg accgggttct cgctgcggcg gacaaactgg
     4561 ccgacttccc gcgactctat gaagtggact cacgctatgg cgaggacgtt cgccggatca
     4621 gtctgatggg acagcatgtc ctctatgatg ttgatgacca gaccagaaaa gtccgggttc
     4681 tggccgtggt cggccagcgc cagaatcccc atattgttct ctaataaccg gtgacgggga
     4741 cgcaattcat tgcgtggccg tcgccggttg gacaagcaca gcgcgtcagc gttttcccca
     4801 cggccaccac cggcgcggtg ttccggccgg ggtctctgct gtctccggtt tgtgttccag
     4861 cagtctcatg gactgcattt cctgccgtag cccggccacc tcctccctga gtgcttttat
     4921 ctccgcgatc aacgcgtcgg aggccatttt ttcagtctca cccgacagtc tcaactcgtc
     4981 ccgctcgtga gtctcatcct gtcttaactc accgtacacc cggataagct cgctggtatc
     5041 caccacccgg ccgccatcag cttccgtacg gtatgatacg cggcctttcg ccatatcccg
     5101 atacagggaa cttcgggctt tacccgtcag ttccgtcgcc tgtctcaccg tcacccacgc
     5161 cattgtctca ccccgctatt tttgagacag tatacctgac aggcgcacac catgcccgga
     5221 aggaaagaat catgacaaaa caggaaaaaa cagccctcaa tatggctaaa tttattaagg
     5281 agcagtcgct gctattgctc gataaactga atgagctgga tatggatacc ccggccgaca
     5341 tgtgcgagcg gctacacgag gatgcggaaa cactctggca ggtgatgcgg gataagctcg
     5401 gggaggaata acagcaccgg catggccggt gctggcattt attttttgcc gaacggccac
     5461 caccggcggg caggtttgtc ctccggcagt tcaggtaccg gcacgttaaa atagcggctg
     5521 ttatattccc gcatcagctc cgctttcagc ttctcgttca ccgcgctttc cccgtgccgc
     5581 tcactcacca cgtcaatcat cgcgttaatc tgctccggtg agcacttccc ggcatcaacc
     5641 acctgcacac cgttctcgat ggcgaaccgc tccaccttgc gatagaggta atcacacagc
     5701 ttcattcctg tgggtttgtt ccgttcccat tgcggatttt taccagtgaa aaagtacgtg
     5761 gctttttcgt cgctgttgag ccgctcgtca tacatcccga tggatttcat catcgccaga
     5821 tgctgggccc ggtcgacaaa acagaaaaca tacgccccgg tctgccaggc gagcaccacc
     5881 atatcttcca gatgctcgct ggtcggcatg atgcagcgct cgccgttccg gtcctccgcg
     5941 tcttcaataa ataccatgta cagcgccata atccctcact ccgttgtcat aaatttaaag
     6001 cacgcaaaca accgttcacg atccagctct gccgttttgc gggcctcgcc ggtctgtggg
     6061 ttcacgattt catccaccga caggattgcc cgctgcaccc tgacctgcgt cgcccggtcc
     6121 gggcagatgt accagatttc atcgatgccg ttagctttgc ggttgaacag atacccggac
     6181 accacttcct gataccggcg acggctcttg acggtgcgct ccacctcaaa cgccaccgtt
     6241 ttaccgtcca gctccgccag ggcgtccggc actttcatgc ctttaagact cagacggtgc
     6301 agaatgcgcc agccggtggc ccctctggct tccatatcaa ggcgcaggga ttgcacggcc
     6361 agccggtgcg gcagatgtgc tgccgacacc cttgacggct gaaacggaat aacatccgcc
     6421 agcggttcgt cctcgtcgaa cgagaacgcc acgccatgcg gcgtcagccc ccacacgctg
     6481 acctgacggc cgtccaccgg atatttggtc cggaccagca gccctttcgc caccaggctg
     6541 tcgaggaagc ggtagaccgg ctgcaccgcc gcaatcccca gccgccgcgc aaccagctcc
     6601 gccgtggaac acagctcttc ccgcaaccag cgcagggtca gccgggtttt ctccgcgttc
     6661 cgcgcctgtc gtttcgcgtg gctgacgttg tacagctcca gcgccatcag taaatccccg
     6721 caaaatcatc atccgctttc ggcccgccgc cgtcggccgg gggccggaac tccgccaggg
     6781 gatcggtttt cgccagcacc gggaaacgct gcaacgggat ttcccggcgc tcaacccgca
     6841 gcggactgct gaacgccatc cgggccacac ccagcccggt catgaccgcg aacccgtccg
     6901 gcaggtgttg cagggtgttc acgtcaaaca gcggccgcgc ctccttctgg atgaagctgt
     6961 ccccgctgat atgctccacg ttccccgcct cccggctggc acggcggcgc tccacgtcca
     7021 ccctgatttc cccggtctgc cccgccgccc actgcgcgga ctccaggctg gccgcccggt
     7081 aaaaccagcg gatcggcgtg ttatccagta ccgtggtttt cacaaattca gcgggcagat
     7141 cctgcccgca ctgcccgaaa tcgcccagcg actggtgcgc gagcaacaga ttgcagtccc
     7201 ggctgcgcag cgtccccagt gcgttcagta catacttcga cagcagatat ttaatttcat
     7261 cgagcatcac gctgacgtgt ggccactccc ggaactcgtc ccgcgcaatg atgatttgcg
     7321 cgcagcgggc aaacagcatt ttttgcacac ggatcaccgc ctcgtcgtcc atcgagccga
     7381 taacgtacag gcagccgccg ccgttgataa tccccgccac atcgatgcca ctgtccgtct
     7441 gcaggacgct caggtcagcg acgttttcca gcctagtgat caaccccttc acgtcctttt
     7501 tcagggcctc cggcagcgcg tacgccgccg ccagcacctg ctgaatgttt gccccctgcg
     7561 gaaactgctc cgcaatcagc ttcgccgctt tctgctcgct gatgcggtag tggtccgcca
     7621 cgtcgccggt ctcgcccagg ttaagcccgg caatcagcag gtttttcagc gcgtactggt
     7681 cgagatcgcg gaacagattc agctgcgccg gtttccccgg ccgcagatcc agcagggtga
     7741 agtccggaca gtgcgcccgg aagacgtgcg gcgcccatgc gtcccctttg gggtcgaagt
     7801 acaccaccgc gtccccgaaa tcgcgtacgc agcgaatgag cgcgttggtg gccatcacgc
     7861 tcttgccgct cccgggcagg ccgaggatct gaatgttggt cttgcgccag gtatcacgcg
     7921 gaaccagcac cggcttgccc tgctcatcct tacccagaaa caccgcctgc tcctgccggg
     7981 cctgctccga ggaaaaatcg gccagcgcat taaccggtac cggctccccg aaatgcaggt
     8041 ggcgcacgtc ggtcatctct cctgtccgct ggctgctgcg ccggctctcc tgattcagtt
     8101 tcgcgttcac cgcctcgtca gcagccgcaa aatgaccgtc aagcagcagg aacgccagca
     8161 gccgcgccaa cacaatcccg acagccagcc cgcccgcgca ccaggcaatc ccgaacaggt
     8221 tagccggctg cagcaggtaa taatctgcgt ttgcgtagag gtccggacgc aggacggttt
     8281 ctccccagct ctcgataaac acggcgacaa ccagcccccc cagaagggcc agaccggccg
     8341 cgccggtcag ccagtacgcg tagttccaga catgtcgacg tgccctgacc atccgcccgg
     8401 gcgaatccag cgaggccagc accgaaaatg gcgctggccg tttattatcg cgcttatagc
     8461 gatcggcata ctcccgtcgc tccttatgac tcagccccac aaaacgcatc cgtccggccc
     8521 aaagttcagc cagaccgaaa aacaggccga taactaggaa agccccggcc agaccgtaat
     8581 actcctggta cagccagctg acccacggat aaatctgccg ggccagctcc ggaatgtgcc
     8641 acttccaggc aagcgaatcc gtattaaaca tgttctctct ccttcagacg atggtttgcc
     8701 ggcgtgatgt gcagcccccg ctgcacaagc cgcggacatg ccccaaggca tggcggcggc
     8761 ggtcacgtcc gtggtgattg cgttcagctt tcaccacgga ggccagcctc tttccgcccc
     8821 atgcggaaaa ggcgcagccc cggaggaaca acgagcacgc cccagcgtgt gagaggaaaa
     8881 ggcggcaccc cagtgcggtc ttttccggtg ttccggcccc cgtcagagtt cgccatagcg
     8941 agcggctgac cacccgcaaa atcaggagca gctttgctgc ttcgccaggt gcgtccagca
     9001 ccgtggattt ttgtgggtgg tcaggcgtga gcctgacggg ggccgggtga atttctggcg
     9061 tggccggcgt ccagccggac ggagccagaa agagggggag agaccgtgcg tcggtggcgt
     9121 ccagccagcg taagcagggg atatccccct gaggtcagcc acgccctcgc ggggatgacc
     9181 tgtatttctc cgtaaggaga aaaaagcaac agagataggt taacgtaaaa tgttcagcga
     9241 aaaaccgata tgattcacct atcctggtaa tttattatca gggaatataa agacttaatc
     9301 aggggacaat atgccacatc agcgcgtccg gtgtccgtct tgtaggtgcc ggggaaattg
     9361 cgcctcctcg ctcactcggt cgctacgctc cggccgtgag actgcggcgg gcgctgcgga
     9421 cacatgcaaa gttacccaca gattccgtga ataagcaggg gactaacatg tgaggcaaaa
     9481 cggcaaggcc acgcaggtgg cgtttttcca taggctccgc cctcctgact gagttcacat
     9541 aaacagacgc tttttcggtg catctgtggg agccggtggc tcaaccatga atcagtcagg
     9601 agtggcgaaa cccgacagga cttaaagatc atcaccgttt ccgatgaatc gctccctctt
     9661 gcgctctcct gttccaaccc tgccgtttcc cggatacctg ttccgccttt ctcccttgcg
     9721 ggaagtgtgg cgctttctca tagctcacac tctggtatct cggctcggtg taggtcgttc
     9781 gctccaagct gggctgtaag caagaacccc ccgttcagcc cgactgctgc gccttatccg
     9841 gtaactgttc acttgagtcc aacccggaaa agcacgcaaa acgccactgg cagcagccat
     9901 tggtaactgg gagttcgcag aggatttgtt tagctaaaca cgcggttgct cttgaagtgt
     9961 gcgccaaagt ccggctacac tggaaggaca gatttggttg ctgtgctctg cgaaagccag
    10021 ttaccacggt taagcagttc cccaactgac ttaaccttcg atcaaaccac ctccccaggt
    10081 ggttttttcg tttacagggc aaaagattac gcgcagaaaa aaaggatctc aagaagatcc
    10141 tttgatcttt tctactgaac cgcgatcccc gtcagttcag aggaggacga tggtgccacc
    10201 gttccaccct gaacatcccg gatatagttt gcctgacatg cgaccagaag aaagcctgaa
    10261 aaaatcaata taaaaagaaa aacaaccttt acctttttca tataccttcc ctgtatttaa
    10321 aaaccccggt attcatccct gaacaccgga agtctgtatt tcaaatcatc cttgataact
    10381 tttgcttacc agttaccatc ccggtaaaca aaggatacaa agtaatcaaa tttactaata
    10441 tcgattacat ttttaaagtg tggctgtaat atcgaaaccc atgatttttc aacatcaaac
    10501 caaccattat taatattatc ctttaaaggc atccccagac tttcaatgac agaaccatca
    10561 tcaccgaagt cttttgagta ttcaccacct ttaaactctt cggttttctt atcaaaccaa
    10621 tgaatatgta atttaagccc catatttacc tcttaaagat attttttaat gtttcgtttt
    10681 ggatccggcc ctttaacctg cttacccgtt tttggatcga atgccccgag gtgttcgcca
    10741 tcactggcgc ggtacccttc aagctcaccg tgctgggaat cccattcata aattttacgc
    10801 tttttatcac cataccatcg ggcccgctta ccaccaccac cttgtttagg ggttttaggt
    10861 ttaccctctt tcaactctcc caacccctta atatcttcag tcttgggatc aggaaaataa
    10921 tcatggccgt aatctttagt tcccttacga ggttttttct tttcctcatt taatttattt
    10981 tctgcatcct tcgctttttt ctccttttgt ttccggctct ccatcgcccc ggaaagcgca
    11041 gccttatccc tggataagga ttcattcatt tcgtttaaag atgtctgggc agcttttaat
    11101 ttctcattct gattctgaat atccttatca agatagcgaa cctgatcctg ccacccggag
    11161 ccatattcat gaggcgggtt ataagtcaca aaatcatctt tatttgcctc tgcctctttt
    11221 acctttttat cggcttcctg aacagcagcc cttgcaccgg ggatcgcctt ctctgtcgat
    11281 gcaatgcgac tgtttaaggt cgcaatattt ttatcttcag catccagctc agctttcgct
    11341 ttatcatact ctcttttcag cccctcttcc gggtgagcag cgtcccatgc ctgctggcga
    11401 cgcttttctt cctccagacg ctgttttacc tgtgccggag taggggtctg acgctcagtg
    11461 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    11521 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    11581 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    11641 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    11701 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    11761 agggcactgt tgcaaagtta gcgatgaggc agccttttgt cttattcaaa ggccttacat
    11821 ttcaaaaact ctgcttacca ggcgcatttc gcccagggga tcaccataat aaaatgctga
    11881 ggcctggctt tgcgtagtgc acgcatcacc tcaatacctt tgatggtggc gtaagccgtc
    11941 ttcatggatt taaatcccag cgtggcgccg attatccgtt tcagtttgcc atgatcgcat
    12001 tcaatcacgt tgttccggta cttaatctgt cggtgttcaa cgtcagacgg gcaccggcct
    12061 tcgcgtttga gcagagcaag cgcgcgacca taggcgggcg ctttatccgt gttgatgaat
    12121 cgcgggatct gccacttctt cacgttgttg aggattttaa cccagaaacc ggtatgcagc
    12181 tttgctgtta cgacgggagg agagataaaa atcgacagtg cggccccggc tgtcgacggc
    12241 ccggtacaga tacgcccagc ggccattgac ctcacgtagg tttcatccat gtgccacggg
    12301 caaagatcgg aagggttacg ccagtaccag cgcagccgtt tttccatttc aggcgcataa
    12361 cgctgaaccc agcggtaaat cgtggagtga tcgacattca ctccgcgttc agccagcatc
    12421 tcctgcagct cacggtaact gatgccgtat ttgcgtacca gcgtacggcc cacagaatga
    12481 tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg cagctccatc agcaaaaggg
    12541 gatgataagt ttatcaccac cgactatttg caacagtgcc gtttccaaaa ggtcgttgat
    12601 caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc
    12661 actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt
    12721 cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc
    12781 gatcaccgct tccctcatga tgtttaacgt ttgacatgag gggcggccaa gggcgccagc
    12841 ccttggacgt ccccctcgat ggaagggtta ggcaacactg cgtgttcgct cgaatgcctg
    12901 gcgtgtttga accatgtaca cggctggacc atctggggtg gttacggtac cttgcctctc
    12961 aaaccccgct ttctcgtagc atcggatcgc tcgcaagttg ctcggcgacg ggtccgtttg
    13021 gatcttggtg acctcgggat cattgaacag caactcaacc agagctcgaa ccagcttggt
    13081 tcccaagcct ttgcccagtt gtgatgcatt cgccagtaac tggtctattc cgcgtactcc
    13141 tggatcggtt tcttcttccc accatccgtc cccgcttcca agagcaacgt acgactgggc
    13201 atacccaatc ggctctccat tcagcattgc aatgtatgga gtgacggact cttgcgctaa
    13261 aacgcttggc aagtactgtt cctgtacgtc agcaagtgtc gggcgtgctt cttctccgcc
    13321 ccaccactcg acgatatgag atcgatttag ccactcatag agcatcgcaa ggtcatgctc
    13381 agtcatgagg cgcagtgtga cggaatcgtt gctgttggtc acgatgctgt actttgtgat
    13441 gcctaacttt gtttggaaat gttgaatact catactcttc ctttttcaat attattgaag
    13501 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    13561 acaaataggg gttccgcgca cttttccccg aaaagtgcca cctgacgtct aagaaaccat
    13621 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga
    13681 attttataaa ccgtggagcg ggcaatactg agctgatgag caatttccgt tgcaccagtg
    13741 cccttctgat gaagcgtcag cacgacgttc ctgtccacgg tacgcctgcg gccaaatttg
    13801 attcctttca gctttgcttc ctgtcggccc tcattcgtgc g
//
