LOCUS       NZ_CP026710             9294 bp    DNA     circular CON 30-NOV-2023
DEFINITION  Citrobacter koseri strain AR_0024 plasmid unitig_1_pilon, complete
            sequence.
ACCESSION   NZ_CP026710
VERSION     NZ_CP026710.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04014865
            Assembly: GCF_002947675.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter koseri
  ORGANISM  Citrobacter koseri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Conlan,S., Thomas,P.J., Mullikin,J., Frank,K.M. and Segre,J.A.
  TITLE     Whole genome sequencing of Citrobacter koseri AR_0024
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Segre,J.A. and Mullikin,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2018) NIH Intramural Sequencing Center, 5625
            Fishers Lane, Rockville, MD 20852, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3
            Genome Coverage                   :: 197x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/30/2023 14:49:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,579
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 24 of 51
            Pseudo Genes (incomplete)         :: 31 of 51
            Pseudo Genes (internal stop)      :: 10 of 51
            Pseudo Genes (multiple problems)  :: 11 of 51
            Pseudo Genes (short protein)      :: 1 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP026710.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Citrobacter koseri"
                     /mol_type="genomic DNA"
                     /strain="AR_0024"
                     /db_xref="taxon:545"
                     /plasmid="unitig_1_pilon"
     gene            complement(join(8307..9294,1..977))
                     /locus_tag="AM351_RS24250"
                     /old_locus_tag="AM351_24220"
     CDS             complement(join(8307..9294,1..977))
                     /locus_tag="AM351_RS24250"
                     /old_locus_tag="AM351_24220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(2458..2607)
                     /locus_tag="AM351_RS24260"
                     /old_locus_tag="AM351_24230"
     CDS             complement(2458..2607)
                     /locus_tag="AM351_RS24260"
                     /old_locus_tag="AM351_24230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(2692..2949)
                     /locus_tag="AM351_RS24265"
                     /old_locus_tag="AM351_24235"
     CDS             complement(2692..2949)
                     /locus_tag="AM351_RS24265"
                     /old_locus_tag="AM351_24235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(2959..4644)
                     /locus_tag="AM351_RS24270"
                     /old_locus_tag="AM351_24240"
     CDS             complement(2959..4644)
                     /locus_tag="AM351_RS24270"
                     /old_locus_tag="AM351_24240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5047..5466
                     /locus_tag="AM351_RS24280"
                     /old_locus_tag="AM351_24250"
     CDS             5047..5466
                     /locus_tag="AM351_RS24280"
                     /old_locus_tag="AM351_24250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            5772..6035
                     /locus_tag="AM351_RS24285"
                     /old_locus_tag="AM351_24255"
     CDS             5772..6035
                     /locus_tag="AM351_RS24285"
                     /old_locus_tag="AM351_24255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            6025..6324
                     /locus_tag="AM351_RS24290"
                     /old_locus_tag="AM351_24260"
     CDS             6025..6324
                     /locus_tag="AM351_RS24290"
                     /old_locus_tag="AM351_24260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6386..6763)
                     /locus_tag="AM351_RS24295"
                     /old_locus_tag="AM351_24265"
     CDS             complement(6386..6763)
                     /locus_tag="AM351_RS24295"
                     /old_locus_tag="AM351_24265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            6832..7011
                     /locus_tag="AM351_RS24300"
                     /old_locus_tag="AM351_24270"
     CDS             6832..7011
                     /locus_tag="AM351_RS24300"
                     /old_locus_tag="AM351_24270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7039..7569)
                     /locus_tag="AM351_RS24305"
                     /old_locus_tag="AM351_24275"
     CDS             complement(7039..7569)
                     /locus_tag="AM351_RS24305"
                     /old_locus_tag="AM351_24275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7576..8307)
                     /locus_tag="AM351_RS24310"
                     /old_locus_tag="AM351_24280"
     CDS             complement(7576..8307)
                     /locus_tag="AM351_RS24310"
                     /old_locus_tag="AM351_24280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     BGC             1..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1777..2355
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7580..8307)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 agattcagct gcgccggttt ccccggccgc agatccagca gggtgaagtc cggacagtgc
       61 gcccggaaga cgtgcggcgc ccatgcgtcc cctttggggt cgaagtacac caccgcgtcc
      121 ccgaaatcgc gtacgcagcg aatgagcgcg ttggtggcca tcacgctctt gccgctcccg
      181 ggcaggccga ggatctgaat gttggtcttg cgccaggtat cacgcggaac cagcaccggc
      241 ttgccctgct catccttacc cagaaacacc gcctgctcct gccgggcctg ctccgaggaa
      301 aaatcggcca gcgcattaac cggtaccggc tccccgaaat gcaggtggcg cacgtcggtc
      361 atctctcctg tccgctggct gctgcgccgg ctctcctgat tcagtttcgc gttcaccgcc
      421 tcgtcagcag ccgcaaaatg accgtcaagc agcaggaacg ccagcagccg cgccaacaca
      481 atcccgacag ccagcccgcc cgcgcaccag gcaatcccga acaggttagc cggctgcagc
      541 aggtaataat ctgcgtttgc gtagaggtcc ggacgcagga cggtttctcc ccagctctcg
      601 ataaacacgg cgacaaccag cccccccaga agggccagac cggccgcgcc ggtcagccag
      661 tacgcgtagt tccagacatg tcgacgtgcc ctgaccatcc gcccgggcga atccagcgag
      721 gccagcaccg aaaatggcgc tggccgttta ttatcgcgct tatagcgatc ggcatactcc
      781 cgtcgctcct tatgactcag ccccacaaaa cgcatccgtc cggcccaaag ttcagccaga
      841 ccgaaaaaca ggccgataac taggaaagcc ccggccagac cgtaatactc ctggtacagc
      901 cagctgaccc acggataaat ctgccgggcc agctccggaa tgtgccactt ccaggcaagc
      961 gaatccgtat taaacatgtt ctctctcctt cagacgatgg tttgccggcg tgatgtgcag
     1021 cccccgctgc acaagccgcg gacatgcccc aaggcatggc ggcggcggtc acgtccgtgg
     1081 tgattgcgtt cagctttcac cacggaggcc agcctctttc cgccccatgc ggaaaaggcg
     1141 cagccccgga ggaacaacga gcacgcccca gcgtgtgaga ggaaaaggcg gcaccccagt
     1201 gcggtctttt ccggtgttcc ggcccccgtc agagttcgcc atagcgagcg gctgaccacc
     1261 cgcaaaatca ggagcagctt tgctgcttcg ccaggtgcgt ccagcaccgt ggatttttgt
     1321 gggtggtcag gcgtgagcct gacgggggcc gggtgaattt ctggcgtggc cggcgtccag
     1381 ccggacggag ccagaaagag ggggagagac cgtgcgtcgg tggcgtccag ccagcgtaag
     1441 caggggatat ccccctgagg tcagccacgc cctcgcgggg atgacctgta tttctccgta
     1501 aggagaaaaa agcaacagag ataggttaac gtaaaatgtt cagcgaaaaa ccgatatgat
     1561 tcacctatcc tggtaattta ttatcaggga atataaagac ttaatcaggg gacaatatgc
     1621 cacatcagcg cgtccggtgt ccgtcttgta ggtgccgggg aaattgcgcc tcctcgctca
     1681 ctcggtcgct acgctccggc cgtgagactg cggcgggcgc tgcggacaca tgcaaagtta
     1741 cccacagatt ccgtgaataa gcaggggact aacatgtgag gcaaaacggc aaggccacgc
     1801 aggtggcgtt tttccatagg ctccgccctc ctgactgagt tcacataaac agacgctttt
     1861 tcggtgcatc tgtgggagcc ggtggctcaa ccatgaatca gtcaggagtg gcgaaacccg
     1921 acaggactta aagatcatca ccgtttccga tgaatcgctc cctcttgcgc tctcctgttc
     1981 caaccctgcc gtttcccgga tacctgttcc gcctttctcc cttgcgggaa gtgtggcgct
     2041 ttctcatagc tcacactctg gtatctcggc tcggtgtagg tcgttcgctc caagctgggc
     2101 tgtaagcaag aaccccccgt tcagcccgac tgctgcgcct tatccggtaa ctgttcactt
     2161 gagtccaacc cggaaaagca cgcaaaacgc cactggcagc agccattggt aactgggagt
     2221 tcgcagagga tttgtttagc taaacacgcg gttgctcttg aagtgtgcgc caaagtccgg
     2281 ctacactgga aggacagatt tggttgctgt gctctgcgaa agccagttac cacggttaag
     2341 cagttcccca actgacttaa ccttcgatca aaccacctcc ccaggtggtt ttttcgttta
     2401 cagggcaaaa gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2461 ctgaaccgcg atccccgtca gttcagagga ggacgatggt gccaccgttc caccctgaac
     2521 atcccggata tagtttgcct gacatgcgac cagaagaaag cctgaaaaaa tcaatataaa
     2581 aagaaaaaca acctttacct ttttcatata ccttccctgt atttaaaaac cccggtattc
     2641 atccctgaac accggaagtc tgtatttcaa atcatccttg ataacttttg cttaccagtt
     2701 accatcccgg taaacaaagg atacaaagta atcaaattta ctaatatcga ttacattttt
     2761 aaagtgtggc tgtaatatcg aaacccatga tttttcaaca tcaaaccaac cattattaat
     2821 attatccttt aaaggcatcc ccagactttc aatgacagaa ccatcatcac cgaagtcttt
     2881 tgagtattca ccacctttaa actcttcggt tttcttatca aaccaatgaa tatgtaattt
     2941 aagccccata tttacctctt aaagatattt tttaatgttt cgttttggat ccggcccttt
     3001 aacctgctta cccgtttttg gatcgaatgc cccgaggtgt tcgccatcac tggcgcggta
     3061 cccttcaagc tcaccgtgct gggaatccca ttcataaatt ttacgctttt tatcaccata
     3121 ccatcgggcc cgcttaccac caccaccttg tttaggggtt ttaggtttac cctctttcaa
     3181 ctctcccaac cccttaatat cttcagtctt gggatcagga aaataatcat ggccgtaatc
     3241 tttagttccc ttacgaggtt ttttcttttc ctcatttaat ttattttctg catccttcgc
     3301 ttttttctcc ttttgtttcc ggctctccat cgccccggaa agcgcagcct tatccctgga
     3361 taaggattca ttcatttcgt ttaaagatgt ctgggcagct tttaatttct cattctgatt
     3421 ctgaatatcc ttatcaagat agcgaacctg atcctgccac ccggagccat attcatgagg
     3481 cgggttataa gtcacaaaat catctttatt tgcctctgcc tcttttacct ttttatcggc
     3541 ttcctgaaca gcagcccttg caccggggat cgccttctct gtcgatgcaa tgcgactgtt
     3601 taaggtcgca atatttttat cttcagcatc cagctcagct ttcgctttat catactctct
     3661 tttcagcccc tcttccgggt gagcagcgtc ccatgcctgc tggcgacgct tttcttcctc
     3721 cagacgctgt tttacctgtg ccggagtaag aacatccgtc accgacacat agaccggttt
     3781 ctgtccgctc tctttcggga aacgaataac ggcttcacgg gagttaccac cggccgtaaa
     3841 accagccgga cgtgaatcac ctttttcagc aataatgcct tttggcgggg ctttcgcggc
     3901 cggaacacct ttaggtacgc tcacctgcag agacgggaga cccggaatcg acacactgaa
     3961 tacccccgga cgttttgtcg gtttcgcatc caccacagga acactcattg gccggccggt
     4021 gacaaccgca atatgctgcc gctcgtcctt cacaatatcc gccacacgtt tagtgacgct
     4081 gaccgtcgcc tggtccagcg gcagggagct gaccggcgtc tccgttaccg tgtctgccgg
     4141 caatgacgtc acgatttttg acatcatttt cggatcatct tttgctatct cagaaggcag
     4201 cactccgtaa atcgcaatcc cccacagacc aaacttaaac ggacctttca gggcagccag
     4261 cacatcagca acagcggccg acaacgcatc gccggaaacg gataaagcca gtccttcagc
     4321 ccccggagtg gccaaggcag gcagaccgaa ggccatcgcg gtggccgaag actgtccgcc
     4381 accagagctc ccgctatgat tgccgctgtt accgccatta ccaccgggtt tactcccgcc
     4441 gttaccaaac tcccccgggt tatagttatg aatatccccg ttaggtgtat gcgtcgtacc
     4501 ccagcccggc ccgctgtttg gatccgtacc accacctgat gaagggccac ctttacctga
     4561 tgtaccgttc acattcccac tggcctgacc accattgtgt cccagacctg aattacccgg
     4621 acctcgtccg tctccgccac tcatatactt ttcctctttg acttttaaaa caataaacta
     4681 aaataaaata ctgtacatat aaccactggt tttatataca gtataaaaat tacgctgcct
     4741 tgtttttcat gtcaagtctg taaatttgcg tttttgtgaa aatgatcaaa aaaacagcca
     4801 ctcacaccat gcgcaatccc ctccagatgc aacaaaaacc agcacaaacg gcgccgttct
     4861 ccggcctcag tatgttttac tgaaatcatt atatgagata ccagaaagcc ccctccggga
     4921 caggaaaaga aacggggggt ctggttcggg ttgttcggtc actgaacacg gtcagaaact
     4981 gatgataaga tagcctgcgg atatataact caatgagaaa aaagaattaa aggtaaggag
     5041 acgctcatga gacgttccgg cagcaaactg tgtcttatcg ccatgataac agcgacagca
     5101 actctcactg cctgtgctcc aaagggcagc gtggaacagc atacccggca ttacgtatat
     5161 gcttcagatg acggttttga tcccaatttt tccacccaga aagctgacac aacacgaatg
     5221 atggtgcctt ttttccggca gttctgggat atgggcgcaa aggataaagc gaccggaaaa
     5281 tcacggagtg atgtgcagca acgcattcag cagtttcaca gtcaggaatt tttaaactca
     5341 ctccaaggca caacacaatt tgcgggtacc gattaccgca gcaaagacct cacaccgaaa
     5401 aaatcaaggc tactggctga caccatttca gctgtttatc tcgatggcta tgaaggcaga
     5461 cagtaagtgg atttaccata acgcgtcatt acgcgcaccg ctcagacggg ctcagggcgt
     5521 ttccggcggc tacgggataa aacaggcaac aacccccccg gaaaactgcc ggaaacgcgc
     5581 ccgataaatt ttatcctaat gcatgcctat gcacgcaact gcatggcata tgttttgacg
     5641 taaaatggat gccgtaagcc cttttttacc gactttctgc ttcatcagcc tgactgacac
     5701 gaccatattc tttcagaccg ccccctgcta aacttccttc atattgccgc attaacccgg
     5761 gaggctccgc aatgagcaac actacacact acgaaaacgc caactttctt cgggaactgg
     5821 ctgaaagcct gccccgcata ctgccggaag gtggcccgga caaagctgcg ctgttgcagc
     5881 gactggctaa cgaagaactg gcccaggcag agtatgaaga tcaggttcgc gccaaagtga
     5941 ccgccgcccg tgctgacacg cgtccgggta tgaccacgga acaactccgc cagcgcctgc
     6001 atggccgtta tcaggaactg cgcgatgccg tatgacgtgg aatggaagca gggagccatc
     6061 gaggatgtaa cgacgctgtt tgactatatc gcggaaaatt catccctgtg ggatgcgcag
     6121 aacgttaccg accgggttct cgctgcggcg gacaaactgg ccgacttccc gcgactctat
     6181 gaagtggact cacgctatgg cgaggacgtt cgccggatca gtctgatggg acagcatgtc
     6241 ctctatgatg ttgatgacca gaccagaaaa gtccgggttc tggccgtggt cggccagcgc
     6301 cagaatcccc atattgttct ctaataaccg gtgacgggga cgcaattcat tgcgtggccg
     6361 tcgccggttg gacaagcaca gcgcgtcagc gttttcccca cggccaccac cggcgcggtg
     6421 ttccggccgg ggtctctgct gtctccggtt tgtgttccag cagtctcatg gactgcattt
     6481 cctgccgtag cccggccacc tcctccctga gtgcttttat ctccgcgatc aacgcgtcgg
     6541 aggccatttt ttcagtctca cccgacagtc tcaactcgtc ccgctcgtga gtctcatcct
     6601 gtcttaactc accgtacacc cggataagct cgctggtatc caccacccgg ccgccatcag
     6661 cttccgtacg gtatgatacg cggcctttcg ccatatcccg atacagggaa cttcgggctt
     6721 tacccgtcag ttccgtcgcc tgtctcaccg tcacccacgc cattgtctca ccccgctatt
     6781 tttgagacag tatacctgac aggcgcacac catgcccgga aggaaagaat catgacaaaa
     6841 caggaaaaaa cagccctcaa tatggctaaa tttattaagg agcagtcgct gctattgctc
     6901 gataaactga atgagctgga tatggatacc ccggccgaca tgtgcgagcg gctacacgag
     6961 gatgcggaaa cactctggca ggtgatgcgg gataagctcg gggaggaata acagcaccgg
     7021 catggccggt gctggcattt attttttgcc gaacggccac caccggcggg caggtttgtc
     7081 ctccggcagt tcaggtaccg gcacgttaaa atagcggctg ttatattccc gcatcagctc
     7141 cgctttcagc ttctcgttca ccgcgctttc cccgtgccgc tcactcacca cgtcaatcat
     7201 cgcgttaatc tgctccggtg agcacttccc ggcatcaacc acctgcacac cgttctcgat
     7261 ggcgaaccgc tccaccttgc gatagaggta atcacacagc ttcattcctg tgggtttgtt
     7321 ccgttcccat tgcggatttt taccagtgaa aaagtacgtg gctttttcgt cgctgttgag
     7381 ccgctcgtca tacatcccga tggatttcat catcgccaga tgctgggccc ggtcgacaaa
     7441 acagaaaaca tacgccccgg tctgccaggc gagcaccacc atatcttcca gatgctcgct
     7501 ggtcggcatg atgcagcgct cgccgttccg gtcctccgcg tcttcaataa ataccatgta
     7561 cagcgccata atccctcact ccgttgtcat aaatttaaag cacgcaaaca accgttcacg
     7621 atccagctct gccgttttgc gggcctcgcc ggtctgtggg ttcacgattt catccaccga
     7681 caggattgcc cgctgcaccc tgacctgcgt cgcccggtcc gggcagatgt accagatttc
     7741 atcgatgccg ttagctttgc ggttgaacag atacccggac accacttcct gataccggcg
     7801 acggctcttg acggtgcgct ccacctcaaa cgccaccgtt ttaccgtcca gctccgccag
     7861 ggcgtccggc actttcatgc ctttaagact cagacggtgc agaatgcgcc agccggtggc
     7921 ccctctggct tccatatcaa ggcgcaggga ttgcacggcc agccggtgcg gcagatgtgc
     7981 tgccgacacc cttgacggct gaaacggaat aacatccgcc agcggttcgt cctcgtcgaa
     8041 cgagaacgcc acgccatgcg gcgtcagccc ccacacgctg acctgacggc cgtccaccgg
     8101 atatttggtc cggaccagca gccctttcgc caccaggctg tcgaggaagc ggtagaccgg
     8161 ctgcaccgcc gcaatcccca gccgccgcgc aaccagctcc gccgtggaac acagctcttc
     8221 ccgcaaccag cgcagggtca gccgggtttt ctccgcgttc cgcgcctgtc gtttcgcgtg
     8281 gctgacgttg tacagctcca gcgccatcag taaatccccg caaaatcatc atccgctttc
     8341 ggcccgccgc cgtcggccgg gggccggaac tccgccaggg gatcggtttt cgccagcacc
     8401 gggaaacgct gcaacgggat ttcccggcgc tcaacccgca gcggactgct gaacgccatc
     8461 cgggccacac ccagcccggt catgaccgcg aacccgtccg gcaggtgttg cagggtgttc
     8521 acgtcaaaca gcggccgcgc ctccttctgg atgaagctgt ccccgctgat atgctccacg
     8581 ttccccgcct cccggctggc acggcggcgc tccacgtcca ccctgatttc cccggtctgc
     8641 cccgccgccc actgcgcgga ctccaggctg gccgcccggt aaaaccagcg gatcggcgtg
     8701 ttatccagta ccgtggtttt cacaaattca gcgggcagat cctgcccgca ctgcccgaaa
     8761 tcgcccagcg actggtgcgc gagcaacaga ttgcagtccc ggctgcgcag cgtccccagt
     8821 gcgttcagta catacttcga cagcagatat ttaatttcat cgagcatcac gctgacgtgt
     8881 ggccactccc ggaactcgtc ccgcgcaatg atgatttgcg cgcagcgggc aaacagcatt
     8941 ttttgcacac ggatcaccgc ctcgtcgtcc atcgagccga taacgtacag gcagccgccg
     9001 ccgttgataa tccccgccac atcgatgcca ctgtccgtct gcaggacgct caggtcagcg
     9061 acgttttcca gcctagtgat caaccccttc acgtcctttt tcagggcctc cggcagcgcg
     9121 tacgccgccg ccagcacctg ctgaatgttt gccccctgcg gaaactgctc cgcaatcagc
     9181 ttcgccgctt tctgctcgct gatgcggtag tggtccgcca cgtcgccggt ctcgcccagg
     9241 ttaagcccgg caatcagcag gtttttcagc gcgtactggt cgagatcgcg gaac
//
