LOCUS       NZ_CP026582            10060 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain WCHKP095649 plasmid p1_095649, complete
            sequence.
ACCESSION   NZ_CP026582
VERSION     NZ_CP026582.3
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06019522
            Assembly: GCF_001887985.4
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Yao,Z., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain WCHKP095649
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Yao,Z., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-FEB-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 10060)
  AUTHORS   Yao,Z., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   4  (bases 1 to 10060)
  AUTHORS   Yao,Z., Feng,Y., McNally,A. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   5  (bases 1 to 10060)
  AUTHORS   Yao,Z., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2016) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu 610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 09:30:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,611
            CDSs (total)                      :: 5,491
            Genes (coding)                    :: 5,371
            CDSs (with protein)               :: 5,371
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 49 of 120
            Pseudo Genes (incomplete)         :: 72 of 120
            Pseudo Genes (internal stop)      :: 16 of 120
            Pseudo Genes (multiple problems)  :: 17 of 120
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP026582.3.
            On Aug 18, 2021 this sequence version replaced NZ_CP026582.2.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP095649"
                     /isolation_source="Secreta"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p1_095649"
                     /geo_loc_name="China: Sichuan"
                     /lat_lon="30.64155078 N 104.06053988 E"
                     /collection_date="2015-11-12"
                     /collected_by="Zhihong Yao"
     gene            complement(join(9987..10060,1..184))
                     /locus_tag="BT120_RS00005"
                     /old_locus_tag="BT120_00065"
     CDS             complement(join(9987..10060,1..184))
                     /locus_tag="BT120_RS00005"
                     /old_locus_tag="BT120_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(194..1879)
                     /locus_tag="BT120_RS00010"
                     /old_locus_tag="BT120_00060"
     CDS             complement(194..1879)
                     /locus_tag="BT120_RS00010"
                     /old_locus_tag="BT120_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            2206..2451
                     /locus_tag="BT120_RS00015"
                     /old_locus_tag="BT120_00055"
     CDS             2206..2451
                     /locus_tag="BT120_RS00015"
                     /old_locus_tag="BT120_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            2725..3096
                     /gene="tolA"
                     /locus_tag="BT120_RS00020"
                     /old_locus_tag="BT120_00050"
     CDS             2725..3096
                     /gene="tolA"
                     /locus_tag="BT120_RS00020"
                     /old_locus_tag="BT120_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            3093..3458
                     /locus_tag="BT120_RS27925"
     CDS             3093..3458
                     /locus_tag="BT120_RS27925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            3633..3911
                     /locus_tag="BT120_RS00030"
                     /old_locus_tag="BT120_00040"
     CDS             3633..3911
                     /locus_tag="BT120_RS00030"
                     /old_locus_tag="BT120_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            3899..4213
                     /locus_tag="BT120_RS00035"
                     /old_locus_tag="BT120_00035"
     CDS             3899..4213
                     /locus_tag="BT120_RS00035"
                     /old_locus_tag="BT120_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4377..4805
                     /locus_tag="BT120_RS00040"
                     /old_locus_tag="BT120_00030"
     CDS             4377..4805
                     /locus_tag="BT120_RS00040"
                     /old_locus_tag="BT120_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            4831..5010
                     /locus_tag="BT120_RS00045"
                     /old_locus_tag="BT120_00025"
     CDS             4831..5010
                     /locus_tag="BT120_RS00045"
                     /old_locus_tag="BT120_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5037..5567)
                     /locus_tag="BT120_RS00050"
                     /old_locus_tag="BT120_00020"
     CDS             complement(5037..5567)
                     /locus_tag="BT120_RS00050"
                     /old_locus_tag="BT120_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5574..6305)
                     /gene="mobC"
                     /locus_tag="BT120_RS00055"
                     /old_locus_tag="BT120_00015"
     CDS             complement(5574..6305)
                     /gene="mobC"
                     /locus_tag="BT120_RS00055"
                     /old_locus_tag="BT120_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(6305..8269)
                     /locus_tag="BT120_RS00060"
                     /old_locus_tag="BT120_00010"
     CDS             complement(6305..8269)
                     /locus_tag="BT120_RS00060"
                     /old_locus_tag="BT120_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(9753..9902)
                     /locus_tag="BT120_RS00065"
                     /old_locus_tag="BT120_00070"
     CDS             complement(9753..9902)
                     /locus_tag="BT120_RS00065"
                     /old_locus_tag="BT120_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1..6879
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          9072..9650
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5578..6305)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 atggctgtag taaaggaacc cattctttct tcaaatcaaa ctcaccatta ttaatattat
       61 cctttaaagg cattcccaag ctttcaatga cagaaccatc gtcaccaaag tctttagaat
      121 actcttcccc tttaaactct tcagttttct tatcaaacca tgtcaaacgt aatttaagcc
      181 ccatatttac ctctcaaaga tattttttaa tgttccgttt tggatctggc cctttaacct
      241 gcttacccgt tttgggatca aatgcgccta ggtgttcgcc atcactggca cgataccctt
      301 caagctcacc gtgctgggaa tcccattcat aaattttacg ttttttatcg ccataccatc
      361 gggcccgctt accaccacca ccttgtttag gtgttttagg atcacctcgc tttaactctc
      421 caagtccctt tatgtcttct gttttaggta ctggatgata atcatggccg tagtctttag
      481 tccccttgcg aggcttgttc ttttcctcat ttaacttatt ttctgcatcc ttcgcttttt
      541 tctccttttg tttccggctc tccatcgccc cggtaagtgc agccttatct ctggataagg
      601 attcattcat tgcgtttaaa gatgcctgag ccgcttttaa tttctcattc tgattctgaa
      661 tatccttatc aagatagcga acctgatcct gccacccgga gccatattca tgaggcgggt
      721 tataagtcac aaaatcatct ttatttgcct ctgcctcttt taccttttta tcggcttcct
      781 gaacagcagc ccttgcaccg gggagcgcct tctctgtcga tgcaatgcgg ctgtttaagg
      841 tcgcaatatt tttatcttcg gcatccagct cagctttcgc tttatcatag tctcttttca
      901 gcccctcttc cggatgagcg gcgtcccatg cctgctggcg acgcttttct tcctcctgac
      961 gctgttttac ctgtgccggg gtaagaacat ctgtcaccga cacataaacc ggcttctgtc
     1021 cggtctcttt cgggaaacga ataacggcct cacgggagtt accaccggct gtaaaaccag
     1081 ccggacgtga atcacctttt tcagcaacaa tgccttttgg cggggctttc gctgtcggaa
     1141 cacctttcgg tacgctcacc tgcagagacg ggagacccgg aatcgacaca ctgaataccc
     1201 ccggacgttt tgtcggtttc gcatccacca cagggacact cattggccga cctgcgacaa
     1261 ccgcaatatg ctgccgctca tccttcacca catccacaac ccgttgtctg acacgaaccg
     1321 tcgcctggtc cagtggtaaa gtacttgccg gagtctccgt caccgcatcg gccggtaatg
     1381 acgtcataat ttttgacatc attttcggat catcttttgc aatctcagaa ggcagcacac
     1441 cgtagatcgc aatcccccac agaccaaact taaacggccc cttcagggca gccagcacat
     1501 cagcaacggc ggccgacaac gcatcgccgg aaactgataa agccagtcct tcagcccccg
     1561 gagtagccag ggcaggcaga ccgaaggcca tcgcagtggc cgaagactgt ccgccaccag
     1621 agctaccgct atgattgccg ctgttaccac cattaccacc gggtttactc ccgccgttac
     1681 caaactcccc cggattatag ttatgaatat ctccattcgg cgtatgcgtc gtaccccagc
     1741 ccggcccgct gtttggatcc gtaccaccac ctgatgaagg gccaccttta ccagacgtac
     1801 cgttcacatt cccactggcc tgaccaccat tatgtcccag acctgaatta cccggacctc
     1861 gaccgtctcc accactcata ccgttttcct ctttgacatt taaaacaata aattaaagcc
     1921 aacatactgt acatatacac agtataatta ttaccttact cctcccgttt tcaggtgtca
     1981 atatatgaga ttagttctca tcttcaggca caaaaaagcg cccggagcca cctgtaagca
     2041 gatcgcgtct tttcccgaaa atttctcatg gcaacaaatt ttattattta agacattcat
     2101 cacacttaca tttcagatgg tatagacaca tctgaaagct aactggcatg aggagaggac
     2161 ctgcggttaa actgttcggg aaacctgaga cgcccggagg ccactatgac gcacctgaaa
     2221 ctgactcccg ctgaagaaaa gcgcctggcg gacgcctttg tcgctcaggc actggccatg
     2281 ggcctgaaag ggaacaaaac gctgccaaag gcggtaaaac aacaggtgcg ggaaagggtg
     2341 cttcgtgcca ttcaggacga acgtgaagcc aggcgggcgc aggaaatgcg tcgggagaaa
     2401 tacgaggcta cggaaaccac cttcacctgg cagccacagc gccgccggta aggggtattt
     2461 accataacgt cgcattgccc gcaccgctca gacggtctga gggcgtttcc ggcggctacg
     2521 ggataaaaca ggcaacaaac cacccggaaa accgccggaa acgcgcccgg taaattgtaa
     2581 cccgctgcat gcctatgcac gcaactgcat ggcgggccgt ttggtgtaaa aacgatacga
     2641 taagccgtgt ttatgtaaat gaacgtggcc gtaaagagtg acaaatatac atgtaacatt
     2701 ttttactgac aggtcatcaa cccgatgcgt ttaataccct ttatcctgct ggcattgtgc
     2761 agccccttac tcctgacggc ctgtaagcat tccaccaccc agccgccgaa aatttatact
     2821 ctggataagt catctaaaat cagtagctac gctgcggcca tcaagaccgc tgttgaaatg
     2881 cagatgtttg aaccagaggc ctatgcggga aaaatgtgca ccgtgcggat atccatacaa
     2941 cgtgatggca ctctgaacag cgccattgct gaaggaggag atccgaaact ctgtaaagca
     3001 gcaatatcag ccgttaccca cgccaaaatt ccgccggcac cggatgaaaa tacctggcaa
     3061 atattcaaaa acgcccctct ggatttcaga ccatgaaagc ttttactctg acagtgctgg
     3121 cggccatgtt cctcactccg gtacatgcga taccactaaa acccgtaacc acactgcagc
     3181 caacctaccc ggtacaggcc gcagcaaaac gcatcgaagg tcaggtgacc gccacgtttg
     3241 atattctgga cgatggacga gtggacaacg tgtcgcttac cggctgtcac cttttttttc
     3301 gggaaacgct gacggcaatg aggcactggc tatatcccgc agggcacccg gcaatgcgac
     3361 aggttataaa ttttgaattc agtctgaaaa atgacggctt acaggatccg cgaccagtca
     3421 ccacccaaaa gctcaatgat gatggttgcc gccagtaaca ggtcctttaa cccgcaggct
     3481 gcaatagtcc tggatgatga gtgagcctgt agcagccctg attagtggaa gcgaagctga
     3541 aaaatgggca gcaaaagccg tatttctggc aaatagcgcc gccacggatt aaagtagtca
     3601 tgtgtaatac atatgactac aaaggggctt ttatgtcatc agcaacatcc atcaggctcg
     3661 atgaagaact taaggaccgg ctgaaaacgc tggccgatga ccggcaccgc tcagcgcacg
     3721 cgctaatgct ggaggcaatc accgaataca tcgaccggga agagaagcgc agccagtacc
     3781 tgcgggacgg acaggccgca tggcagcact atcaggaaac gggtctgcac cttactgcgg
     3841 aagaagcgga agcctggata agtacctggg gaactgagaa cgagcaggat gctccgccat
     3901 gccacaggta acgatttccg cgctggcaca gcgcgattta caacgccttc aggactttct
     3961 caaaaccaaa aatcggctgg cagccagaaa aggaggtgag gtgatcgtcc gggctatcca
     4021 gcaactgaaa acattgccag acattggccg cccggtgccg tttctgccgc tggaatataa
     4081 ggaactggtg atcgggtttg gcgacagtgg ctatgtgatg ctctaccgcc acgaccggga
     4141 aatggaccag attgtgatcg tcaccgtcag gcatcagaaa gaatccgggt atccgggggc
     4201 agacagcctc taataaccga tgacgggggc gcaattcatt gcgtggccgt cgccggtttg
     4261 acaagcgcag cgcgtcagaa aaccacatcc tgaacggaaa aacgggtaaa attcagcgat
     4321 acatttaccg tgagaaatta cacacctttc acacccgata aaatcagggg tatacagtgg
     4381 cgaaaacatt gctcaacctt tcacaggcag cacaagccgc gggcattacc agaagaacgc
     4441 tatacaatca tgtaaaacag ggaaaggtta ccgtgtcacg tgacgggaaa aacaaccctg
     4501 tcgtggatgt gtcagaactg atccgtgtat acggaaatgt gaatatccct gagaaacaaa
     4561 ttcccggcat ttcacaccgt gaaaatacac aaaaaaattt cccacaggaa cagcttctgg
     4621 caatgcaaaa ggaactggcc gatctccggc aggctgtcac cctgatgctg gaagataaaa
     4681 catcccggga agaagaacgc cgccagcacg atgatgagcg ccggaaactc caggccgaag
     4741 tggacagatt gacaacagaa ctgacacaga agaaaaaacg cttctggtcc ggctggttta
     4801 gctgagaact taccgaaggg aatggagaac atgacgaagc aggaaaaaac tgccctcaat
     4861 atggctaaat tcattaagga tcagtcgctg ctgttgctgg aaaagctgaa cgagctggac
     4921 atggataccc cggccgacat gtgcgaacgg cttcacgagg atgcggaaac actctggcag
     4981 gtgatgcggg ataagctcgg ggaagaataa cagcaccggc catgccggtg ctggcgttat
     5041 tttttgccga acggccacca ccggcgggcg ggtttgtcct ccggcagttc aggcaccggc
     5101 acgttaaaat agcggctgtt atattcccgc atcagctccg ctttcagctt ctcgttcacc
     5161 gcgctttccc cgtgccgctc gctgaccacc tcaatcatcg cgttaatctg ctccggtgag
     5221 cacttcccgg catcaaccac ctgcacaccg ttctcgatgg cgaaccgctc cacctttcga
     5281 tagaggtaat cacacagctt cattcctgtg ggtttgtccc gttcccattg cggattttca
     5341 ccggtgaaaa agtacgtggc tttttcgtcg ctgttgagcc gctcgtcata catcccgatg
     5401 gatttcatca tcgccagatg ctgggcccgg tcgacaaaac agaaaacata cgccccggtc
     5461 tgccaggcga gcaccaccat gtcttccaga tgctcgctgg ttggcataat gcagcgctcg
     5521 ccgtctttgt cttccacgtc ctcaataaat accatgtaca gcgccataat ccctcactcc
     5581 gttgtcataa atttaaagca cgcgaacagc ctctcccggt ccagctccac cgttttgcgg
     5641 gcctcgccgg tctgtggatt cacgatttca tccaccgcca gaatcgcccg ctgtacccgg
     5701 acaagcgtcg gccggtcagg gcagatgtac cagatttcat cgatgccgtt cgcccggcgg
     5761 ttgaacagat acccggacac cacttcctga taccgcctgc ggctcttgac ggtgcgctct
     5821 acctcaaatg ccaccgttct gccgtccagc tccgccagcg cgtccggcac tttcatccct
     5881 ttcagggcca tacggtgcag gtaccgccag ccggtggccc cccggccctc catggagagg
     5941 cgcagggact gcacggccag ccggtgcggc agctgcgccg ccgacaccct tgacggctga
     6001 aacggaataa cgtccgtcag cggctcgtcc tcgtcgaacg agaacgccac cccgtgcggc
     6061 gtcagccccc acacgctgac ctgacggccg tcaaccgggt atttcgcccg gaccagcagc
     6121 cctttcgcca ccagactgtc caggaagcgg taaaccggct gtaccgccgc aatccccagc
     6181 cgtcgcgcaa ccagctccgc cgtggaacac agctcttccc gcagccagcg cagggtcagc
     6241 cgtgttttct ccgcgttccg cgcctgccgc ttcgcgtggc tgacgttgta ctgctccagt
     6301 gtcatcagta aagccccgca aaatcatcat ccgcttttgg tccgccgccg tcggccggag
     6361 gcaggtactc cgccagcggg tccgttttcg ccagcaccgg gaaggttttc agcgaaattt
     6421 cccggcgctc aacccgcagc gggctgctga acgccagccg cgccacgccc agcccggtca
     6481 tcaccgcgaa cccgtcaggc aggtgttgca gggtgttcac gtcaaacagc ggccgcgcct
     6541 ccttctggat gaagctgtcg ccgctgatgt gctccacgtt ccccgcctcc cggctggccc
     6601 ggcggcgctc cacgtccacc cggatctccc cggtctggcc cgccgcccac tgcgcggact
     6661 cctggctggc cgcgcgatag aaccagcgga tcggcgtgtt atccagtacc gtggttttca
     6721 caaaatcagc gggcagatcc tgcccgcact gaccgaagtc gcccagcgac tggtgcgcga
     6781 gcagcaggtt gcagtcccgg ctgcgcagcg tccccagcgc gttcagcacg tacttcgaca
     6841 gcagatattt aatctcatcg agcatgatgc tcgcgtgcgg ccacctccgg aactcatccc
     6901 gcgcaatgat gatttgcgcg cagcgggcaa acagcatctt ctgcacccgg atcaccgcct
     6961 catcgtccat cgagccgatg acgtacaggc agccgccgcc gttgattatc cccgccacat
     7021 cgataccgct gtccgtctgc aggacgctca ggtcggccac gttctccagc ttggtgatca
     7081 gccccttcac gtcctttttc agggcctccg gcaacgcgta cgccgccgcc agcacctgct
     7141 gaatgtttgc cccctgcggg aactgctccg caatcagctt cgccgctttc tgctcgctga
     7201 tgcggtagtg gtccgccacg tcgccggtct cactcaggtt aaacccggca accagcagat
     7261 tcttcagggc gtactggtcg agatcgcgga acagattcag ctgcgccggt ttccccggcc
     7321 gcagatccag cagggtgaag tccgggcagt gcgcccggaa gacgtgcggc gcccacgcgt
     7381 cccctttggg gtcgaagtac accacagcgt ccccgaaatc gcgtacgcag cgaatgagcg
     7441 cgttggtggc catcgcgctc ttgccgctcc cgggcaggcc gaggatctga atattggtct
     7501 tgcgccaggt atcacgaggg accagcaccg gctggccctg ctcatcctta cccagaaaca
     7561 ccgcctgctg tttccgggcc tgctccgtgg aaaaatcggc cagcgcgtta accggtaccg
     7621 gctccccgaa atgcaggtgg cggacgtctg tcatttctcc ggtccgctgg ctgctgcgcc
     7681 ggctctcctg attcagtttc gcgttcaccg cctcgtcagc ggccgcaaaa tgaccatcaa
     7741 gcagcaggaa cgccagcagc cgcgccagaa ggacgccggc gacaagccct ccggcacacc
     7801 aggctatccc gaacaggtta gccggctgca gcaggtaata atctgcgttc gcatagaggt
     7861 ccgggcgcag gacggtttct ccccagctct cgataaacac ggcgacaacc agccccccca
     7921 gaagggccag accggcagcg ccggtcagcc agtacgcgta gttccagaca tgtcgccgtg
     7981 ccctgaccat ccgcccgggt gaatccagcg aggccagcac cgaaaatggc acagtccggt
     8041 tattatcgcg cttatagcga tcggcatact cccgccgcgc cttatggctc agccccacaa
     8101 aacgcattcg gccggcccag agttcagcca gaccgaaaaa caggccgata accagaaaaa
     8161 aaccggccag actgtaatac tcccggtaca gccagctgac ccacgggtaa atctgccggg
     8221 ccagctccgg gatatgccac tgccaggcaa gcgaatccgt attaaacatg caatctctct
     8281 ccttcagacg acgggtttac cggcgtgatg cgcagccccc gctgcacaag ccgcggacat
     8341 gccccaaggc atggcggcgg cggtcacgtc cgtggtgatt gcgttcagct ttcaccgcgg
     8401 aggccagcct ctttccgccc catgcggaaa aggcgcagcc ccggaggaac aacgagcacg
     8461 ccccagcgtg tgagaggaaa aggcggcacc ccagtgcggt cttttccggt gttccggccc
     8521 ccgtcagagt tcgccatagc gagcggctga ccacccgcaa aatcaggagc agctttgctg
     8581 cttcgtcagg tgcgtccagc accgtggatt tttgtgggtg gtcaggcgtg agcctgacgg
     8641 gggccgggtg aatttctggc gtggccggcg tccagccgga cagagccaga aagaggggaa
     8701 aagaccgtgc gtcggtggcg tccagccagc gtaagcaagg tcttttcccc tgaagtcagc
     8761 cacgccccag cggggatgac ttgcatttct ccgcaaggag aaaaaagcaa cagagatagg
     8821 ttaacgtaaa atgttcagcg aaaaaccgac atggttcacc tatcctgata attgatcgtc
     8881 agggaataga aagacttaat caggggacaa tatcccacat cagcgcgtcc ggtgaccgtc
     8941 ttgcaggtgc cggggaaatt gcgcctcctc gctcactcgg tcgctacgct ccgggcgtga
     9001 gactgcggcg ggcgctgcgg acacatacaa agttacccac agattccgtg gataagcagg
     9061 ggactaacat gtgaggcaaa acggcagggc cgcgccggtg gcgtttttcc ataggctccg
     9121 ccctcctgcc agagttcaca taaacagacg ctttttcggt gcatctgtgg gagccgtgag
     9181 gctcaaccat gaatctgaca gtacgggcga aacccgacag gacttaaaga tccccaccgt
     9241 ttccggtagg tcgctccctc ttgcgctctc ctgttccgac cctgccgttt accggatacc
     9301 tgtccgccgt tctcccttgc gggaagtgtg gcgctttctc atagctcaca cactggtatc
     9361 tcggctcggt gtaggtcgtt cgctccaagc tgggctgtaa gcaagaactc cccgttcagc
     9421 ccgactgctg cgccttatcc ggtaactgtt cacttgagtc caacccggga aagcacgcaa
     9481 aacgccactg gcagcagcca ttggtaactg ggagttcgca gaggatttgt ttagctaaac
     9541 acgcggttgc tcttgaagtg tgcgccaaag tccggctaca ctggaaggac agatttggtt
     9601 gctgtgctct gcgaaagcca gttaccacgg ttaagcagtt ccccaactga cttaaccttc
     9661 gatcaaacca cctccccagg tggttttttc gtttacaggg caaaagatta cgcgcagaaa
     9721 aaaaggatct caagaagatc ctttgatctt ttctactgaa ccgcgatccc cgtcagttca
     9781 gaggaggacg atggtgccac cgttccgccc tgaacatccc ggatataatt tgcctgacat
     9841 gcggccagaa gaaagccagc aacaatcaat ataaaaagaa aaatagtctt tacctttttc
     9901 atatactttc cctgtattta aagactccgg cattcatccc tgaacaccgg aagtcagtat
     9961 ttcaaatcat ccttgataat tttttgttac cagttaccgt cccgataatc aaaagctata
    10021 taacaattat ccacatcagg ttttatttga tttttaaaaa
//
