LOCUS       NZ_CP026575             2444 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Escherichia coli strain WCHEC005237 plasmid p4_005237, complete
            sequence.
ACCESSION   NZ_CP026575
VERSION     NZ_CP026575.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06209723
            Assembly: GCF_002945135.3
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Feng,Y., McNally,A. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain WCHEC005237
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Feng,Y., McNally,A. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 2444)
  AUTHORS   Feng,Y., McNally,A. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002945135.3-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 03:28:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,106
            CDSs (total)                      :: 4,987
            Genes (coding)                    :: 4,712
            CDSs (with protein)               :: 4,712
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 275
            CDSs (without protein)            :: 275
            Pseudo Genes (ambiguous residues) :: 0 of 275
            Pseudo Genes (frameshifted)       :: 94 of 275
            Pseudo Genes (incomplete)         :: 184 of 275
            Pseudo Genes (internal stop)      :: 58 of 275
            Pseudo Genes (multiple problems)  :: 55 of 275
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP026575.2.
            On Jul 18, 2018 this sequence version replaced NZ_CP026575.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WCHEC005237"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p4_005237"
                     /geo_loc_name="China: Sichuan, Chengdu"
                     /collection_date="2014-10"
                     /note="also known as WCHEC1237 or 1237"
     gene            335..817
                     /locus_tag="C4E05_RS00105"
                     /old_locus_tag="C4E05_00110"
     CDS             335..817
                     /locus_tag="C4E05_RS00105"
                     /old_locus_tag="C4E05_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223375251.1"
                     /translation="MNTKKISRLERHTYMDENGEVKQDSKIETFSIPAEPPYVKLYIAD
                     IAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVD
                     KQIFFRAGRGELEANPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVMDDEC"
                     /name="hypothetical protein"
     gene            804..1034
                     /locus_tag="C4E05_RS00110"
                     /old_locus_tag="C4E05_00115"
     CDS             804..1034
                     /locus_tag="C4E05_RS00110"
                     /old_locus_tag="C4E05_00115"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_124788667.1"
                     /translation="MMNAEDKLFKNVDRLMLHDSLKKNEVLTVWERTFLSSVYSIYRRT
                     SGNKYSTKGLSPKQKNAAFSILKKHNYHLND"
                     /name="hypothetical protein"
     gene            complement(1104..2072)
                     /gene="mobV"
                     /locus_tag="C4E05_RS00115"
                     /old_locus_tag="C4E05_00120"
     CDS             complement(1104..2072)
                     /gene="mobV"
                     /locus_tag="C4E05_RS00115"
                     /old_locus_tag="C4E05_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     Typing          complement(1108..2081)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.077"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 aagcggaatt tccactcccc gtacatagaa atgcgtacac tccttgctaa aaaaaggcag
       61 tttttcagtg tctaaaaatg ccgtttttca gcccttattt tcccccttaa tatatagata
      121 tagatatggg aacccgcttg taccaagggc tagagcgaag aatcgtactc acctcaagta
      181 ccaatcgtac tcacctcaag tactaatcgt actcacctca aggactaatc gtactcacct
      241 caagtactaa tcgtacttga tacaaaggac ttgactgtat gtctctttag tacttaaatg
      301 cgagtactaa atcatggcga aacgtggggc tggagtgaat acgaaaaaga tttctaggct
      361 tgaacggcat acatacatgg atgaaaacgg tgaggtaaaa caagactcta agatcgagac
      421 cttttccatc ccagccgaac ctccctacgt caagctatac attgcagaca ttgcgatgat
      481 tcatggttta tcaaaatctc aaaatgatgt gcttttacaa ttggcaatgc tgattggatg
      541 ggatgggttt gtgtctgtat caaaaactag atttgaaaaa accatacagc ccagggttca
      601 tattgaatat cagaccttta aaaacataat tgcggctctt gtagataaac aaatattctt
      661 ccgggcaggg cgtggggaac ttgaagctaa cccctttctt tttgcacggg gagactggcc
      721 atcaatttac gaaagaagaa agaaaattga gttaaagatc agttatgaca ccaatggaag
      781 aacggtgcag acgaaggtaa tggatgatga atgctgaaga taaacttttt aaaaatgtag
      841 acaggctaat gctgcatgat tcactaaaaa agaatgaggt tctgactgtt tgggagcgta
      901 cttttttaag cagtgtttac agcatatatc gtagaactag cggtaataaa tactcaacta
      961 agggtttgtc accaaagcag aaaaacgcag ctttttctat tttaaaaaaa cataattacc
     1021 atctgaatga ttaatttttt cgtggtgaat gccgcaaatg acgtcacttt gcggcgttag
     1081 ccctatcctg catcgcatca aaactaacgc tccataccac ccatgccacg cgatcgcgtc
     1141 tgtcgctgtt tttcacgttc aatacgctgc tgtttttcct tttcctggtt ttgctgctta
     1201 aaccgctgag ccatcatcag gatttcggtc atctgctctc tggaaagccc cttaaaaggc
     1261 tctgtgacgc tctgtaggcg tttctgctgc gcatccatag tcttctgtag ctcacgcgct
     1321 ctttttgcgt tctgggcgct ctgagaggcc atagcgatgg ttcctgcaaa gccatcgttc
     1381 actgcgtggc ttagacgttt ggctatggct gcctgatctt ccacgtcttt ggtgaaaatg
     1441 cccttccgca atacacgcgg ctccagcgcc tccggcgaga tcgatacctg gctacgggtg
     1501 ccacggttta tcgattcgta gtagtgctga actgtctgat gcgtggcgcg gcttccctcg
     1561 atgccgcgct caagacccag tttttttact gattcagcgt aggtgctctg atcttcacgc
     1621 atctttgacc gcccgccaat aaactctttc gcggacagcc gtccatcctg cgtcaatggc
     1681 accacgaacg cagacaggtg cggggtggcc tcgtcccggt gaaccaccgc cgccacaaca
     1741 cgatccttcc cgtatttctt ctccagccat tgctcagagc gggcgaaaaa ctccgcctgc
     1801 tgccgtggtg tggcctcttt ccaccactca gggcttgctg tcataacgta ttcaacggcc
     1861 agtaccgcat ctttacggcg tttttccggc agcagctccc gcacacgccc cattgcctga
     1921 tccgttgatt tggacacaga gcagtagttt tctggtgtcc gttcagcgtt agcgttcggc
     1981 gtctcacgct ctcggtagca gtgctgcaac gcgctggcca cgcttcccat tcccgtcagc
     2041 tttttgcagc gcattatcgc gtagttcgcc atgttccccc ctgattccgg cattcattca
     2101 gccaggcggg ggagcgcatt gcgatacctc cggcgggcca ctatcaagat gatagtgtaa
     2161 actcactaga ctttgctacg caaaatcgtt cgggcagcaa gctgccagaa ggtcaaaatc
     2221 aacggcggtc tggcaggtgg aaggcgtcgc ttcgcgccac cccccaccac tgccaagcct
     2281 gttttatccg gagtgtcgtc aagggtaaat ggcactcgct acgctcgccc ttgacgaaat
     2341 caggcaatga aatcgtcttt gaaagagcag gaaccgctcg ccgcagcttt ggctgaggcg
     2401 taccgcagga gcaacgcgac gaggaacgaa cagccaaagc gagg
//
