LOCUS       NZ_CP026572             6200 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Escherichia coli strain WCHEC005237 plasmid p1_005237, complete
            sequence.
ACCESSION   NZ_CP026572
VERSION     NZ_CP026572.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06209723
            Assembly: GCF_002945135.3
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Feng,Y., McNally,A. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain WCHEC005237
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Feng,Y., McNally,A. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 6200)
  AUTHORS   Feng,Y., McNally,A. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002945135.3-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 03:28:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,106
            CDSs (total)                      :: 4,987
            Genes (coding)                    :: 4,712
            CDSs (with protein)               :: 4,712
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 275
            CDSs (without protein)            :: 275
            Pseudo Genes (ambiguous residues) :: 0 of 275
            Pseudo Genes (frameshifted)       :: 94 of 275
            Pseudo Genes (incomplete)         :: 184 of 275
            Pseudo Genes (internal stop)      :: 58 of 275
            Pseudo Genes (multiple problems)  :: 55 of 275
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP026572.2.
            On Jul 18, 2018 this sequence version replaced NZ_CP026572.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WCHEC005237"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p1_005237"
                     /geo_loc_name="China: Sichuan, Chengdu"
                     /collection_date="2014-10"
                     /note="also known as WCHEC1237 or 1237"
     gene            complement(214..363)
                     /locus_tag="C4E05_RS25645"
     CDS             complement(214..363)
                     /locus_tag="C4E05_RS25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            934..1092
                     /locus_tag="C4E05_RS26525"
                     /old_locus_tag="C4E05_00050"
     CDS             934..1092
                     /locus_tag="C4E05_RS26525"
                     /old_locus_tag="C4E05_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            1552..2367
                     /gene="sul2"
                     /locus_tag="C4E05_RS00020"
                     /old_locus_tag="C4E05_00040"
     ARG             1553..2364
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            2428..3231
                     /gene="aph(3'')-Ib"
                     /locus_tag="C4E05_RS00025"
                     /old_locus_tag="C4E05_00035"
     ARG             2429..3228
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3231..4067
                     /locus_tag="C4E05_RS00030"
                     /old_locus_tag="C4E05_00030"
     CDS             3231..4067
                     /locus_tag="C4E05_RS00030"
                     /old_locus_tag="C4E05_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="WP_094885524.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYSGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="APH(6)-I family aminoglycoside O-phosphotransferase"
     gene            complement(<4174..4578)
                     /locus_tag="C4E05_RS00035"
                     /old_locus_tag="C4E05_00025"
                     /pseudo=""
     CDS             complement(<4174..4578)
                     /locus_tag="C4E05_RS00035"
                     /old_locus_tag="C4E05_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(4622..5011)
                     /locus_tag="C4E05_RS00040"
                     /old_locus_tag="C4E05_00020"
     CDS             complement(4622..5011)
                     /locus_tag="C4E05_RS00040"
                     /old_locus_tag="C4E05_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            5229..5669
                     /locus_tag="C4E05_RS00045"
                     /old_locus_tag="C4E05_00015"
     CDS             5229..5669
                     /locus_tag="C4E05_RS00045"
                     /old_locus_tag="C4E05_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     ARG             3232..4064
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.64"
                     /coverage="100.0"
     Typing          complement(399..572)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          496..1101
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cccgccatgg ttgattattt ttcaacctca aaatggtcat acacctaacg cagccgcgat
       61 tttcatcgca actgcatgaa ttaaaagaaa aaaaaggaat atttcgtttt cacgagtaat
      121 acccgggtat taaaaatcca ctgtaattac atgaaaataa agaaataaaa ggtaataccg
      181 gagtattacc acgtacgcgg gcaagcgaaa aagctataac gcttcccggc cgacaggcga
      241 cccaaaatcc acaggggcat gcaggttctc ttcagtaacc tgcgccatta aatcaaaaag
      301 gtcatattcg ccgggaagct gcgtatcagt cgaatccagt tgttcatcat attcagatgg
      361 catggggtga tatccagtag ctgagaggtt gttagcgggt ttcggggcgc agccctgaac
      421 cagtcatgta gcgctagcgg agtgtatact ggcttactat gttgccacga ttgcgaaaat
      481 cagtgaagtg cttcatgtgg caggagaaaa aaggcagcac cggcgcgtca gcagaatatg
      541 cgatacagga gatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcggctg
      601 cggcgagcgg taccggctta cggacgggga cggaaatttc ctggaagata ccaggaagac
      661 acttagcaaa gaagagataa ggccacggcg aagccgtttt tccataggct ccgcccccct
      721 gacaagcatc gcaaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa
      781 agatcccagg cgtttccccc tggtagctcc ctcgtgcgct ctcctgttcc tgcctttcgg
      841 tttaccggtg tcattccgct gttatggccg cggtttctca ttccacgcct gacactcagt
      901 tccgtgaagg cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac
      961 cgctgcgcct tgtccggtaa ctatcaactt gagtccaacc cggaaagaca cgacaaaacg
     1021 ccactggcag cagccattag taacaggagt gcacgagaag atagattaag aggtcttgaa
     1081 gtagaggcct gactgcggct acactgaaag aacagttttg gtaatggcgg tctcgtgaac
     1141 cctgttaccg ggttaagcag ttccccaact gacttaacct tcgaacaaac cgcctcccca
     1201 ggcggttttt tcgtttacag agcaagagat tacgattcat tgaaaaggat ctcaagaaga
     1261 tcctttgtag attactatca attaatcttc cgaacatgac accagtcact gttgattacg
     1321 gttaaaccgc tccgtaacag gtttccctcg tattggtgac ttgcagcacc aaagaagcaa
     1381 aaaagataaa tatatctact tattgagaga tgatatattt atttccaaaa tgctacagat
     1441 acatcatcat gaaacggatc accgcaaaca ggttactcgc ctggggattc cctttcgacc
     1501 cgagcatccg tatgagactc atgctcgatt attattatta tagaagcccc catgaataaa
     1561 tcgctcatca ttttcggcat cgtcaacata acctcggaca gtttctccga tggaggccgg
     1621 tatctggcgc cagacgcagc cattgcgcag gcgcgtaagc tgatggccga gggggcagat
     1681 gtgatcgacc tcggtccggc atccagcaat cccgacgccg cgcctgtttc gtccgacaca
     1741 gaaatcgcgc gtatcgcgcc ggtgctggac gcgctcaagg cagatggcat tcccgtctcg
     1801 ctcgacagtt atcaacccgc gacgcaagcc tatgccttgt cgcgtggtgt ggcctatctc
     1861 aatgatattc gcggttttcc agacgctgcg ttctatccgc aattggcgaa atcatctgcc
     1921 aaactcgtcg ttatgcattc ggtgcaagac gggcaggcag atcggcgcga ggcacccgct
     1981 ggcgacatca tggatcacat tgcggcgttc tttgacgcgc gcatcgcggc gctgacgggt
     2041 gccggtatca aacgcaaccg ccttgtcctt gatcccggca tggggttttt tctgggggct
     2101 gctcccgaaa cctcgctctc ggtgctggcg cggttcgatg aattgcggct gcgcttcgat
     2161 ttgccggtgc ttctgtctgt ttcgcgcaaa tcctttctgc gcgcgctcac aggccgtggt
     2221 ccgggggatg tcggggccgc gacactcgct gcagagcttg ccgccgccgc aggtggagct
     2281 gacttcatcc gcacacacga gccgcgcccc ttgcgcgacg ggctggcggt attggcggcg
     2341 ctgaaagaaa ccgcaagaat tcgttaactg cacattcggg atatttctct atattcgcgc
     2401 ttcatcagaa aactgaagga acctccattg aatcgaacta atattttttt tggtgaatcg
     2461 cattctgact ggttgcctgt cagaggcgga gaatctggtg attttgtttt tcgacgtggt
     2521 gacgggcatg ccttcgcgaa aatcgcacct gcttcccgcc gcggtgagct cgctggagag
     2581 cgtgaccgcc tcatttggct caaaggtcga ggtgtggctt gccccgaggt catcaactgg
     2641 caggaggaac aggagggtgc atgcttggtg ataacggcaa ttccgggagt accggcggct
     2701 gatctgtctg gagcggattt gctcaaagcg tggccgtcaa tggggcagca acttggcgct
     2761 gttcacagcc tatcggttga tcaatgtccg tttgagcgca ggctgtcgcg aatgttcgga
     2821 cgcgccgttg atgtggtgtc ccgcaatgcc gtcaatcccg acttcttacc ggacgaggac
     2881 aagagtacgc cgcagctcga tcttttggct cgtgtcgaac gagagctacc ggtgcggctc
     2941 gaccaagagc gcaccgatat ggttgtttgc catggtgatc cctgcatgcc gaacttcatg
     3001 gtggacccta aaactcttca atgcacgggt ctgatcgacc ttgggcggct cggaacagca
     3061 gatcgctatg ccgatttggc actcatgatt gctaacgccg aagagaactg ggcagcgcca
     3121 gatgaagcag agcgcgcctt cgctgtccta ttcaatgtat tggggatcga agcccccgac
     3181 cgcgaacgcc ttgccttcta tctgcgattg gaccctctga cttggggttg atgttcatgc
     3241 cgcctgtttt tcctgctcat tggcacgttt cgcaacctgt tctcattgcg gacacctttt
     3301 ccagcctcgt ttggaaagtt tcattgccag acgggactcc tgcaatcgtc aagggattga
     3361 aacctataga agacattgct gatgaactgc gcggggccga ctatctggta tggcgcaatg
     3421 ggaggggagc agtccggttg ctcggtcgtg agaacaatct gatgttgctc gaatattccg
     3481 gggagcgaat gctctctcac atcgttgccg agcacggcga ctaccaggcg accgaaattg
     3541 cagcggaact aatggcgaag ctgtatgccg catctgagga acccctgcct tctgcccttc
     3601 tcccgatccg ggatcgcttt gcagctttgt ttcagcgggc gcgcgatgat caaaacgcag
     3661 gttgtcaaac tgactacgtc cacgcggcga ttatagccga tcaaatgatg agcaatgcct
     3721 cggaactgcg tgggctacat ggcgatctgc atcatgaaaa catcatgttc tccagtcgcg
     3781 gctggctggt gatagatccc gtcggtctgg tcggtgaagt gggctttggc gccgccaata
     3841 tgttctacga tccggctgac agagacgacc tttgtctcga tcctagacgc attgcacaga
     3901 tggcggacgc attctctcgt gcgctggacg tcgatccgcg tcgcctgctc gaccaggcgt
     3961 acgcttatgg gtgcctttcc gcagcttgga acgcggatgg agaagaggag caacgcgatc
     4021 tagctatcgc ggccgcgatc aagcaggtgc gacagacgtc atactagata tcaagcgact
     4081 tctcctatcc cctgggaaca catcaatctc accggagaat atcgctggcc aaagccttag
     4141 cgtaggattc cgccccttcc cgcaaacgac cccaaacagg aaacgcagct gaaacgggaa
     4201 gctcaacacc cactgacgca tgggttgttc aggcagtact tcatcaacca gcaaggcggc
     4261 actttcggcc atccgccgcg ccccacagct cgggcagaaa ccgcgacgct tacagctgaa
     4321 agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc catgctccag
     4381 ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca attcctttcc
     4441 ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct gatagagaag
     4501 ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc tggccgtcct
     4561 ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata cagtctatcg
     4621 cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga cattgccagc
     4681 cagtgcccgt cactcccact cttacattgt tcgcagtatc taggtacatg ttattgcgtg
     4741 caccttcatg atgtataatg ttattaatga cagcagaaag atcgacagaa ttaatattct
     4801 tcgtacctgg gacagttatc gggtgtcggc cagtgatgag accaaaggat gcagtataag
     4861 atgaaccact actattatca tggccaccat taccaccacc gttaaaacta cctcccgaac
     4921 ttccagagcc gctatgacca tcattgtagg ggtcatttgg tcccatgtga ccaaaaccac
     4981 cgacagtacc gctatttaac tctgtactca taaaattatc ctctttgact tttaaaaaca
     5041 gcaaaaactg tatttataac aatgtgttta tatacagtat tcgaaatact acgcccgtca
     5101 aaaccacacc gtcaacaggt tttttgtgta tttttcacgc ctcacgggca gcctcagacc
     5161 ttttgtgtta aaacagtaat atgttctgct gttacaatac ggtacacaca ggagacactg
     5221 tcacttttat ggcatggcac acagttcaag agacactgaa attaacagga aagtcgcgct
     5281 cgcaattata tcgtgacatg gctaaaggcc ttgtcagcta tcgcacagga aaaaatgatc
     5341 gccgggagtt cgagaccagt gaactgattc gggcctatgg cgagctgcgc acttctgaga
     5401 caccaaaaag gcacaaggag ggacactcca aatctgtcgt tagccaggcg gaaaatgctc
     5461 agctagacgt cattcagcag aaactggacg ctctgcagca gactgttacc ctaatgcttg
     5521 aagacaagat ggcgcgggaa gcagaaaacc gcctgcgaaa tgaggagagg gaacaactac
     5581 aggccgaaat tagccgtctg gctgacgcgc tggcacagga aaaaaagcgg ggattctggt
     5641 cgcggctgtt tcgctccggt tcagagtaac agtgcaggtg actctgattt accataacat
     5701 ctgttatcag caccgcctga gaggccctca gggcgtttct gacgggaaac cggtaaaaca
     5761 ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta cggcatgcat
     5821 gagtatgcgc acaagtgcat agcgcggaaa ttcacaccat tcagccgggg taagtcctgt
     5881 ttccggcgtt cttcccatcc gaaaacggtg aacggggacg caatttattg cgtggccgtg
     5941 aagcgttgcc ggcgcagccg gaaaacccgc tgtcagcacg ttcaggaagg gggtgatgta
     6001 caaatggcac agaggatgaa agtgacgtat gagctgaagg aatgatgtaa aagtgatgta
     6061 taagtgatgt aaaagtgatg tatgaactga aggagcgatg taattctgta gtcaggaaga
     6121 ataacccgtt catgcaggca tgaacgatac agtgaggaac gctttttttg tcacgatctc
     6181 cccttaacct gcgtaaatca
//
