LOCUS       NZ_CP026427             8765 bp    DNA     linear   CON 05-JAN-2024
DEFINITION  Acinetobacter sp. ACNIH1 plasmid unnamed, complete sequence.
ACCESSION   NZ_CP026427
VERSION     NZ_CP026427.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03455971
            Assembly: GCF_002934695.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. ACNIH1
  ORGANISM  Acinetobacter sp. ACNIH1
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Weingarten,R.A., Johnson,R.C., Conlan,S., Ramsburg,A.M.,
            Dekker,J.P., Lau,A.F., Khil,P., Odom,R.T., Deming,C., Park,M.,
            Thomas,P.J., NISC Comparative Sequencing Program,C., Henderson,D.K.,
            Palmore,T.N., Segre,J.A. and Frank,K.M.
  TITLE     Genomic Analysis of Hospital Plumbing Reveals Diverse Reservoir of
            Bacterial Plasmids Conferring Carbapenem Resistance
  JOURNAL   MBio (2018) In press
REFERENCE   2  (bases 1 to 8765)
  AUTHORS   Segre,J.A. and Mullikin,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2018) NISC, NIH, 5625 Fishers Lane, Rockville, MD
            20852, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 13:28:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,625
            CDSs (total)                      :: 3,512
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 113
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 91 of 228
            Pseudo Genes (incomplete)         :: 141 of 228
            Pseudo Genes (internal stop)      :: 76 of 228
            Pseudo Genes (multiple problems)  :: 75 of 228
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP026427.1.
            Bacteria available from Karen Frank, Department of Laboratory
            Medicine, National Institutes of Health Clinical Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8765
                     /organism="Acinetobacter sp. ACNIH1"
                     /mol_type="genomic DNA"
                     /strain="ACNIH1"
                     /db_xref="taxon:1636603"
                     /plasmid="unnamed"
                     /geo_loc_name="USA"
                     /lat_lon="38.984652 N 77.094709 W"
                     /collection_date="2014"
     gene            complement(<1..>88)
                     /locus_tag="C3F22_RS18440"
                     /pseudo=""
     CDS             complement(<1..>88)
                     /locus_tag="C3F22_RS18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005198822.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /name="BrnA antitoxin family protein"
     gene            177..839
                     /locus_tag="C3F22_RS18190"
                     /old_locus_tag="C3F22_18200"
     CDS             177..839
                     /locus_tag="C3F22_RS18190"
                     /old_locus_tag="C3F22_18200"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_104427084.1"
                     /translation="MSKLIYFTILNFFLINSVYAENNKFLDMVQSKVKQEMKDPDSTKF
                     QKLREIKNSVGESVACGEVNAKNSYGGYVGYHLFSYSSDGIVILNSPKDSDYYRNKFVY
                     DKSGCAGEKEEFLARNKDMFKNYCSVAYELFTDVIVNNKSNVDAITHAMNNYQKYNLNV
                     FNKDLDKAKIDLLANLEQVLQSKETVKKIKKDKNGMFKAQYMFICPINMEKNYIFEK"
                     /name="hypothetical protein"
     gene            complement(921..1316)
                     /locus_tag="C3F22_RS18195"
                     /old_locus_tag="C3F22_18205"
     CDS             complement(921..1316)
                     /locus_tag="C3F22_RS18195"
                     /old_locus_tag="C3F22_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family toxin"
                     /protein_id="WP_086185555.1"
                     /translation="MIYLLDTNICIYVINHKPQQVFERFKQYQLGQLAISSITASELAF
                     GVEKSGSERNKQALNKFLSPLDILPYDEQAIWHYAKLRQDLQSTGKTIGSLDMLIAAHA
                     LALDVVLVTNNIKEFERIDGLKLENWI"
                     /name="type II toxin-antitoxin system VapC family toxin"
     gene            complement(1313..1543)
                     /gene="vapB"
                     /locus_tag="C3F22_RS18200"
                     /old_locus_tag="C3F22_18210"
     CDS             complement(1313..1543)
                     /gene="vapB"
                     /locus_tag="C3F22_RS18200"
                     /old_locus_tag="C3F22_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="WP_104427085.1"
                     /translation="METAKVFQTGRSQAVRLPKAFRFNGTEVAIKSFGRGVLLMPIENP
                     WDIMLEAINEFEVGFQLERADQGEQLREDFK"
                     /name="vapB"
     gene            2268..2477
                     /locus_tag="C3F22_RS18205"
                     /old_locus_tag="C3F22_18215"
     CDS             2268..2477
                     /locus_tag="C3F22_RS18205"
                     /old_locus_tag="C3F22_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005249157.1"
                     /translation="MFNLNIFNKISSEVLTIKNDLELNSENQLITKYKTSTSEDYKQAI
                     VLIFKERGYTRLEIGQLLREPKAS"
                     /name="hypothetical protein"
     gene            2602..3483
                     /locus_tag="C3F22_RS18210"
                     /old_locus_tag="C3F22_18220"
     CDS             2602..3483
                     /locus_tag="C3F22_RS18210"
                     /old_locus_tag="C3F22_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282326.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISAba9 family transposase"
                     /protein_id="WP_004282326.1"
                     /translation="MSIEEFIIFVYVIIEELYPIVVTQPLRTRGFPPAVTDAEIITMQI
                     VGEFLGLDTDKNIWMYFKNNWLEWFPKLGSYPNFCKHCANLWHVSQSIMAQLIQRYGSD
                     NIHFIDGFPLPVCSYTRARKHKNFKEYAGFSYCAAKQEKYYGFKAHIVINLSGMISGYT
                     FAPAHCDERDVAPEITNDIYGLLGADKGYLRPELKRYYEYQGIDLQTPFRKNMIDFRPK
                     ETLQILMKARRKIETVIGQLTDRFHIQKVRAKDLWHLTHRITRKILSHTICVVLNKKLG
                     HSPIQFENLILS"
                     /name="IS982-like element ISAba9 family transposase"
     gene            3707..5161
                     /locus_tag="C3F22_RS18215"
                     /old_locus_tag="C3F22_18225"
     CDS             3707..5161
                     /locus_tag="C3F22_RS18215"
                     /old_locus_tag="C3F22_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995702.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="WP_005249159.1"
                     /translation="MLSNVREQWFSNIRGDVLAGLVVGLALIPEAIAFSIIAGVDPKVG
                     LYASFCIAVVISFVGGRPAMISAATGAMALLMVTLVKEHGLEYLLAATILTGVIQIMAG
                     YLKLAKLMRFVSKSVVIGFVNALAILIFMAQLPELINVTWHVYALVALGLAIIYLFPMI
                     PKLGKLLPSPLVCIIAITLLAIALGIDVRTVGDMGSLPDTLPMFLIPNIPLNIETLMII
                     LPYSVALAAVGLLESMMTATIVDEMTDTPSDKFQECKGQGIANIASGFMGGMAGCAMIG
                     QSMINVKSGGLTRLSTFSAGIFLLILVVFISDWLKVIPMAALVAVMIMVSISTFEWSSV
                     TQLKDNPKSSNIVMIATVIVVVATHNLALGVLTGVLLSALFLANKLENDIQVIASFEGT
                     ARLYDLRGQIFFSSSEKFMQGFDFKEDVKDVIIDLTHSHIWDVTSVAMLDSVVNKFQKN
                     SVNVTVRGLNEASSIMIDKYGTHARI"
                     /name="SulP family inorganic anion transporter"
     gene            complement(5376..5921)
                     /locus_tag="C3F22_RS18220"
                     /old_locus_tag="C3F22_18230"
     CDS             complement(5376..5921)
                     /locus_tag="C3F22_RS18220"
                     /old_locus_tag="C3F22_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_104427086.1"
                     /translation="MKTSTKYSVSDAATLYKKSRATLYKDIKNGVLSRDHDGSIDFSEL
                     LRVYGEPFGGTNSKRLDTSPRQNIDTPEYTDNTVVELKNQIEFLKKQLEKAEDRETKAN
                     ARIDTLLTLIEMKPPQANISTFTEQQSDQDIATDPRSKKPNEDGLTTQEIKRIPVPEHI
                     EPEPEKRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(5985..6878)
                     /gene="repM"
                     /locus_tag="C3F22_RS18225"
                     /old_locus_tag="C3F22_18235"
     CDS             complement(5985..6878)
                     /gene="repM"
                     /locus_tag="C3F22_RS18225"
                     /old_locus_tag="C3F22_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004734502.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001038666.1"
                     /translation="MNKNHVVKSNQVIEASYQLSAVEQRIVLAAISRIPKNQPITDDEL
                     YPVSINELRQLGVHEKTAYRDLKEGINRLYERSINLSIDDKSIKMRWVQEVQFLDSQSV
                     IGIRFSKPILPFISNLSREFTKYALSDIAGINSGYGIRIYELLVQYRQIGKREISVENL
                     RTMLELGKKYPLFADFKKRVIDTAIDQINECSPLNVTYEQKKTGRKVTSIIFSFKEKTK
                     SISHQNTDVPKEFYKLTDAQINMFGNQLSRLHELSHLAQQGESYDDLAITIKDMLRDPK
                     QQKQFLPYLKNLGFKL"
                     /name="repM"
     gene            complement(7413..8591)
                     /locus_tag="C3F22_RS18230"
                     /old_locus_tag="C3F22_18240"
     CDS             complement(7413..8591)
                     /locus_tag="C3F22_RS18230"
                     /old_locus_tag="C3F22_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_104427087.1"
                     /translation="MASYHFSVKSKNKGYALSHYLYISRLMQYEGIRKTSNETVEHIEP
                     GRCMPSFVKDPIEFWQAADTYERANAKAYMEYEIALPNEFTPDQRKMLVKTFFDKHIVP
                     QQYPHSYAIHNVKSRLSGEEQPHCHLMFSLKADDGIERTAEQYFKRYNPQNPAKGGAKK
                     IQLQDGHADYSTFLLHIRKAWENHLNDALAKYCPTVTYQIDGQDITIKNQVSADNYEKY
                     NEIHGTLYLPEPKLGAGKQNETMEYLAEIQKIRLHNAKEREMEQKQRQQTQQHEHFYSC
                     LNEPYTSLEVMYYLNYLVKKAKNQKELNQDFNEIIKTQKPILQAEHWNLNKMDYQLKNS
                     LQYTDPQIEFNPVTVLKSQVEQPQVVQGNQPTQPSIQPKPKNNDYDFGGPGF"
                     /name="MobA/MobL family protein"
     Typing          complement(5986..6878)
                     /name="rep_cluster_1364"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.888"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7417..8513)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cataggattt tattcatacg tgtttgatat ccttttcctt aagcgattgc ataacgtctg
       61 catcaatgcg tacagtcata acttgcttaa tctacgtatt caatttgggt agaatctaaa
      121 aaatagcatc cacagattca gccatcttat gcaaattgtt aaattatgag gactttatga
      181 gtaaattaat atacttcacc attcttaatt tttttttaat taattcagtt tatgctgaaa
      241 ataataaatt tttagatatg gttcaaagta aagtaaaaca ggaaatgaaa gatccagact
      301 ctacaaaatt tcaaaaatta agggaaataa agaattctgt tggtgagagt gttgcttgtg
      361 gtgaagtaaa tgctaaaaac tcctatggag gatatgttgg atatcattta ttttcgtata
      421 gttcagatgg aattgtaatt ttaaattcgc caaaagatag tgattattat agaaataagt
      481 ttgtttatga caaatctggc tgtgcaggtg aaaaagaaga atttttagct cgaaataaag
      541 atatgttcaa aaattattgt tctgttgcgt atgaactttt tacagatgta atagtaaata
      601 ataaaagtaa tgtagatgct attacgcatg ccatgaataa ttatcaaaaa tataatttga
      661 acgtgtttaa taaagatcta gataaagcaa aaatagattt attagcaaat ttggagcaag
      721 tattgcaatc taaagaaacc gtaaaaaaaa ttaaaaagga caaaaacgga atgtttaaag
      781 cgcaatatat gtttatttgc cctataaata tggaaaaaaa ttatattttt gaaaaataat
      841 ttatgtatga aagtatgtag tttttctaaa attaacataa tggtgcttat acgaaattta
      901 gcccttttaa gggctaaaaa ttagatccag ttctctaact ttaacccatc tatacgctca
      961 aattccttga tgttgtttgt gactagtaca acatctaaag cgagggcatg agctgctatt
     1021 agcatatcta aactaccaat agttttacct gttgattgga gatcttgacg taatttagcg
     1081 taatgccaaa tcgcttgctc atcgtagggt aaaatatcta agggtgataa aaacttgttc
     1141 agagcttgct tattgcgttc agatccagac ttttcaacgc caaaagccag ctctgacgcg
     1201 gttatgctag aaatagctaa ctgtccaagc tgatattgct taaagcgttc aaaaacttgc
     1261 tgtggtttat gattgatcac gtagatacaa atatttgtgt ctaagaggta aatcatttga
     1321 agtcctcacg taattgctca ccctgatcag ctcgttcaag ctgaaatcct acttcaaatt
     1381 cgttaattgc ttctaacatg atgtcccatg ggttttcgat gggcattaat aagacaccac
     1441 ggccaaaact cttgatagca acttcagtac cattaaagcg aaatgcttta ggtaaacgga
     1501 cagcttgact acgacctgtt tgaaaaactt tagcggtttc catatttgcc ccccaataaa
     1561 atagatatca ttgatacata tcataccatc aaactattta ttacacgtga tttattgtaa
     1621 gtaatcgttg ctttctaaaa ttgatctaaa agatacaggc aataaaaaac cccggcatcc
     1681 tgccggggtt tttcgtattg gttcgctcgg tataactcct gtcgtacctt acagtccttg
     1741 tgcttatctc ttgttattct tgtatggaac ttcctgttcc cgatgagttc atcataggaa
     1801 aattcacctc tcctggacag ccgtaaagaa ccggaattta cgtaagccta ggcctacaaa
     1861 atagcctaaa ttttatccac aaaaaatgtg gataatttta agcattcaag cattgataaa
     1921 atttaaaaaa tcgcaaaatc gactactttt taaacaaatt tattggcaaa aactatcaaa
     1981 accgttcata aaaaagccga cttgtttcaa agtcggcttt ttacggattt tagggttgag
     2041 tgtgaaattt taagtcattg atttttatat taatatacaa gtgtacttcg tataaccgcc
     2101 attatgttaa atggatggca tttgggagat attatttatt ctttcaatag gcagaaactt
     2161 gcagaatttt tcttaatttg taatcctttc ggctaagggt gtctagcgta gctaagacag
     2221 gtattagtta tagtcgggcc gctataatac ggatgacgag tgcctgtatg tttaacctaa
     2281 atatctttaa taagatctct agcgaagtat tgaccatcaa aaatgatctt gagctgaatt
     2341 ctgaaaatca actgataact aagtataaga cttctacttc tgaagattat aaacaggcta
     2401 ttgttttgat ttttaaagaa cgtggctata cacgtttgga aataggtcaa ttacttaggg
     2461 agccaaaagc ttcataaaat accgattaaa caaagcttgg atataacatc caggctttac
     2521 cccaatctca actttttaag tgattgaaat aaaaaggaca taaccctatt ttagtttcca
     2581 ccaagaaatt aaagcaaagt tatgtccatt gaagaattta tcatttttgt ctatgtaatc
     2641 atagaagaac tttatcccat tgttgttact cagcccctga gaactcgtgg ttttccgcct
     2701 gctgttactg atgcagaaat catcactatg caaatagtag gtgaatttct tggtttagat
     2761 acagacaaaa atatttggat gtatttcaaa aacaattggt tagaatggtt tccaaaatta
     2821 ggatcttatc ccaatttctg taaacattgt gcaaacctgt ggcatgtcag tcaatccatt
     2881 atggcacagc tcattcagcg ttatggttca gataacattc actttattga tggcttccca
     2941 ctccctgtct gtagttatac cagagctaga aagcataaaa actttaaaga atacgcaggt
     3001 tttagttatt gtgctgcaaa acaagaaaaa tattatggtt tcaaagctca tatcgtcatt
     3061 aatctttcag gcatgatttc gggttatacc tttgcacctg ctcattgtga tgagcgagat
     3121 gttgcacctg aaattacgaa tgatatttac ggtttactgg gagcagataa gggctattta
     3181 agaccagaat tgaaacgata ttatgaatat cagggtattg atttacaaac accctttaga
     3241 aaaaacatga tagatttcag acctaaagag acactgcaaa tactgatgaa agccagaaga
     3301 aagattgaaa cagtgattgg tcaattaaca gatcgctttc acattcagaa agtgagggca
     3361 aaagatctat ggcatttaac ccaccgcata actcgtaaaa ttctgtcgca tacaatttgt
     3421 gtggtgctaa ataaaaagct tggtcattca ccaattcaat tcgaaaatct tattttaagt
     3481 tgagattggc gtaggcttta taaaaaagta cctagctgtt acccttgtcc tgattttttt
     3541 actctaaacc ttctttcatc ttatgacact tacgccacct taagtttatt taccgtctta
     3601 aatctgtaaa taactcatca ctgtctagtg atgtggtgta gtcgcttgtg tgttgtatat
     3661 atcacatcac tagcctatct gaaaaattaa aaaacaggct atgtttatgt tgtctaatgt
     3721 ccgagaacag tggttctcta acatccgtgg ggatgtactt gctggattag tcgtcggtct
     3781 tgcactcatc cctgaagcca tcgcattttc aattattgca ggtgtagatc ctaaagtcgg
     3841 tctatacgca tctttctgta tagccgtcgt gatttctttc gtaggcggtc gtccagctat
     3901 gatttcagca gctacagggg ctatggcact gctcatggtt actttggtca aagagcatgg
     3961 gcttgaatac ctgcttgcag caacaatttt gacgggtgta attcaaataa tggctggcta
     4021 tttaaagctc gccaagttaa tgcgatttgt atctaaatct gtggttattg gattcgtaaa
     4081 tgcgttagca attttaattt ttatggctca gctgcctgaa ctgattaatg tgacctggca
     4141 tgtatatgct ttagttgctt taggtttggc gattatttat ctcttcccta tgattcccaa
     4201 attaggaaaa ttattgcctt ctccacttgt ctgcatcatt gcaatcacat tactggcaat
     4261 tgctctagga attgatgtaa gaactgtggg tgatatggga tcgttacctg acacacttcc
     4321 aatgttcttg attccaaata ttcctttgaa tattgaaaca ttgatgatta ttttgcctta
     4381 ttcagtcgca ctggcggctg taggtttact tgaatcgatg atgacagcaa ccatcgtgga
     4441 tgaaatgaca gatacaccaa gtgataaatt ccaagaatgt aaaggacaag gtatcgcgaa
     4501 tattgcttct ggctttatgg gggggatggc cggttgtgcc atgattggtc aatccatgat
     4561 taacgtgaaa tcgggtggtc ttacacgctt atcaacattc tctgcgggta tttttcttct
     4621 tattctggtt gtatttatca gcgattggct caaagtgatt ccaatggctg cattggtagc
     4681 ggtaatgatc atggtctcta tcagtacctt tgaatggagt tctgtcactc agttaaaaga
     4741 taatcctaag agcagtaata tcgtcatgat tgcgactgtg attgtagttg ttgctacgca
     4801 taacctggct ttgggtgtgt taacaggcgt attactttca gctttgttcc tagcgaataa
     4861 acttgaaaat gatatccaag taatagcatc ttttgaagga acagcccgtt tatatgattt
     4921 gagaggtcag atcttcttta gttcttctga aaaattcatg caagggtttg actttaaaga
     4981 ggatgtaaag gatgtgatta ttgatttgac ccattctcac atttgggatg taacatcagt
     5041 cgccatgctt gattctgttg taaataaatt ccaaaagaat agtgtgaatg tcactgtgcg
     5101 tggcttaaat gaagccagct ctatcatgat tgataagtat ggaacacatg ccagaatcta
     5161 agtgcaggag attttacgtt tatgtagaat ctctaaaatt aacataatac accttatacg
     5221 aaatgcgtta taattctctt gaaaattcaa cgctgtagtt cctagttgat agcccgaaca
     5281 tccctgttcg ggcttttttt atgatttttc acgttccacg cctattcttt aaccaatgtt
     5341 tcacgacttt gttcatatta gttaaacaat cgtggttaat catagggtct aaaaaaacgg
     5401 ctccaaaagc cccttttttc aggctctggt tcaatatgct ccggcactgg aatgcgttta
     5461 atttcttgag tagtcaatcc gtcctcattt ggtttttttg accgaggatc tgttgctata
     5521 tcctggtcag attgctgctc tgtaaacgtg cttatattgg cttgtggtgg cttcatttca
     5581 ataagtgtaa gtagcgtgtc aatacgagca tttgccttcg tttcccgatc ttctgctttt
     5641 tccaactgtt tctttaaaaa ctcaatctga tttttgagtt caacaactgt attatcagtg
     5701 tattcaggtg tatcaatgtt ttgtcttgga gacgtatcaa gacgttttga gtttgttcca
     5761 ccaaaaggct caccatagac acgaagtaat tctgaaaagt cgatagaacc atcatgatca
     5821 cgacttaata ctccgttttt tatatctttg tagagtgtgg cccgagattt tttatacagt
     5881 gttgcagcat cactaacact gtattttgta cttgttttca tattgtatta ctgtgtatta
     5941 acgatacgta ttggagaata caataagtgt attctccaat aaactcaaag tttaaagcct
     6001 aagtttttta gataaggtaa aaactgtttt tgctgttttg gatctctcaa catatcttta
     6061 attgttatag ctaaatcatc atagctttca ccttgctgtg ctagatgtga tagttcatgt
     6121 aaacgagaaa gttgatttcc aaacatatta atttgagcat ctgttagttt ataaaattct
     6181 ttaggtacat ctgtattctg atgactaata ctcttagtct tttctttaaa tgaaaaaata
     6241 atactagtaa ctttacgacc agtctttttc tgttcataag tgacatttaa agggctgcat
     6301 tcgtttattt ggtctatagc agtatcaatt actcgtttct tgaaatctgc gaataaaggg
     6361 tactttttac caagctcaag cattgtccgc aaattctcaa ctgaaatttc acgcttacct
     6421 atttgtcgat attgaacaag tagctcataa atacgaatac cgtacccact atttattcca
     6481 gcgatgtctg acagggcata tttggtgaat tctctactta gattagatat gaaaggcaga
     6541 attggttttg aaaaacgaat accaatgaca ctttgactat ctagaaattg aacttcttgt
     6601 acccatcgca tttttataga cttgtcatca atactgaggt taatagatcg ttcatagagt
     6661 ctattaatac cttcctttaa atctctgtat gcagtttttt catgtacacc taattgcctt
     6721 aactcattga tactaacagg atatagctca tcatctgtaa taggctgatt ttttggaatc
     6781 cgtgaaatgg cagctaagac aattcgttgc tctacggcac ttaattgata tgaggcttct
     6841 attacttgat ttgattttac aacgtgattt ttattcaaaa taaaaccatt aatttttaat
     6901 atatgacaaa tatatacttg tcatagttag tagtcaatcc gtcatagtta agtagtcaat
     6961 ccgtcatagt tagtagtcaa tccgtcatag ttagtagtca atccgtcata gttaagcttt
     7021 aaaaccctta ctgtataagg catacagggg actgaaaaga tttaaaagat ttaaaatata
     7081 tttaaaaaaa gcaaattgaa aaattgccct tggttttcgc tacgctcaaa ctctattgaa
     7141 tctcgctttc gctcgattcg tcggggcaat cttttggtta ataccctcta gaatttaata
     7201 aaaagcaaaa gcagtaggaa ttcgctacgc tcataatttt tttatcttcg cttcgctcag
     7261 tcatgactta aaaatatcaa gtcgctttgc tctgtacttg agtcttagcg acaaaaggga
     7321 gcttgtaatc agggaaaaaa acataacgag aattttgatt gcctcagttc gctcgtagac
     7381 actcgctctg taaatgccta caagattcat attcaaaaac caggaccacc aaaatcgtaa
     7441 tcattatttt tcggcttagg ctgtatggat ggttgagtag gttgattgcc ttgaacaact
     7501 tgaggttgtt caacctgtga ttttaagaca gtaacaggat taaactcgat ttgaggatct
     7561 gtatattgga gtgaattttt tagttggtaa tccatcttat tcaaattcca atgttcggcc
     7621 tgaagtattg gcttctgtgt tttgatgatt tcattgaagt cttggttgag ttctttttgg
     7681 tttttggctt ttttgaccaa gtaattcaag taatacatga cctcgagtga ggtatacggc
     7741 tcattcaggc aagaatagaa atgttcatgc tgttgcgttt gttgccgctg cttttgttcc
     7801 atttctcgtt ctttggcatt gtgcagccgg attttctgga tttcagccag gtattccatg
     7861 gtttcatttt gttttccagc acctaatttg ggttccggga ggtacaaggt gccgtggatc
     7921 tcgttgtatt tctcatagtt gtcggctgat acctggttct tgatggtgat atcttggccg
     7981 tcaatctggt aggtcacagt agggcagtac ttggccaaag catcattgag atggttttcc
     8041 caagctttgc ggatgtgcaa taagaatgtg gagtaatctg cgtggccatc ctgtagctgg
     8101 attttcttgg caccgccttt ggccggattt tgaggattgt agcgtttgaa atattgttct
     8161 gctgtgcgtt caatgccgtc atcagctttg agggagaaca tgaggtggca atgcggctgt
     8221 tcttcgccac tgagacgcga tttaacgttg tgtatggcgt atgaatgcgg atattgctgt
     8281 gggactatgt gtttgtcaaa aaacgtcttt acgagcattt tacgctggtc tggcgtgaac
     8341 tcattgggca gggcaatttc atattccatg taggctttgg cgttggcacg ttcatacgta
     8401 tctgctgctt gccagaattc gataggatct ttaacgaaag aaggcataca acgaccaggt
     8461 tcaatgtgtt ctacggtttc gttgcttgtt ttacggatgc cttcatattg cataagtctg
     8521 gaaatgtaga ggtaatggct aagtgcatag cctttatttt tgcttttgac gctgaaatgg
     8581 tagctggcca tgttcacctt tttgttggtc tgggaccggt ttttatggag tgaaacgaga
     8641 tgaaaaccgc ccggatttgg aatgcctgga tggctgaaaa atccggttga agggggtcaa
     8701 gggggaagcg ccccattgcg tactagtttc tccgattagg agaaactgtc aagtgcgaat
     8761 tattt
//
