LOCUS       NZ_CP026422            13080 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter sp. ACNIH1 plasmid pACI-a283, complete sequence.
ACCESSION   NZ_CP026422
VERSION     NZ_CP026422.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03455971
            Assembly: GCF_002934695.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. ACNIH1
  ORGANISM  Acinetobacter sp. ACNIH1
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 13080)
  AUTHORS   Weingarten,R.A., Johnson,R.C., Conlan,S., Ramsburg,A.M.,
            Dekker,J.P., Lau,A.F., Khil,P., Odom,R.T., Deming,C., Park,M.,
            Thomas,P.J., NISC Comparative Sequencing Program,C., Henderson,D.K.,
            Palmore,T.N., Segre,J.A. and Frank,K.M.
  TITLE     Genomic Analysis of Hospital Plumbing Reveals Diverse Reservoir of
            Bacterial Plasmids Conferring Carbapenem Resistance
  JOURNAL   MBio (2018) In press
REFERENCE   2  (bases 1 to 13080)
  AUTHORS   Segre,J.A. and Mullikin,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2018) NISC, NIH, 5625 Fishers Lane, Rockville, MD
            20852, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 13:28:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,625
            CDSs (total)                      :: 3,512
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 113
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 91 of 228
            Pseudo Genes (incomplete)         :: 141 of 228
            Pseudo Genes (internal stop)      :: 76 of 228
            Pseudo Genes (multiple problems)  :: 75 of 228
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP026422.1.
            Bacteria available from Karen Frank, Department of Laboratory
            Medicine, National Institutes of Health Clinical Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13080
                     /organism="Acinetobacter sp. ACNIH1"
                     /mol_type="genomic DNA"
                     /strain="ACNIH1"
                     /db_xref="taxon:1636603"
                     /plasmid="pACI-a283"
                     /geo_loc_name="USA"
                     /lat_lon="38.984652 N 77.094709 W"
                     /collection_date="2014"
     gene            complement(123..1727)
                     /locus_tag="C3F22_RS16135"
                     /old_locus_tag="C3F22_16145"
     CDS             complement(123..1727)
                     /locus_tag="C3F22_RS16135"
                     /old_locus_tag="C3F22_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611704.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="WP_004733110.1"
                     /translation="MNTLPDLSQLTHEQLLEFTRQLAMQHQSLAQSNQELEKSNQQLDA
                     KVQHLSILTQKYEHELALFKQHKFGSKNEHLTAKQIHLWDEAVEEDIAAVDLELERLNA
                     DKTDAATEKATVNKPKRRLLPDHLHTIRIEHEPASTQCSCGCQLRRIGEDISEKLHFRP
                     AQFYKEQHVRGKWVCDQCDTLTQQAMPAYVIDKGIASPELLSHVLVSKYADHLPLYRQR
                     LIYQRAGIDLSRSTLSDWIGRCGVELEPLANALKEVVLQQRVIHADETPVTIMRMGDDE
                     KKPKKGYVWAYATTQYNPVQAVIYDFQDSRSGQHAEEFLNGWQGHLVCDDYSGYKARFK
                     SGDVIEVGCMAHARRKFHELHVTGKSQIAEQALLMIQKLYAIEAELRKKTDSTAEHRRE
                     YRQQHSQPVMQQLYEWLNQHYLTVPSSSPTAKAINYSLKRWPALSRYLDDGNLPIDNNW
                     AENSMRPWALGRKNWLFAGSLRSGQRAANIMTLIQSAKLNGLDPYAYLSDVLKRLPTHK
                     ATQIEELLPHRWKSNSN"
                     /name="IS66 family transposase"
     gene            complement(1802..2137)
                     /gene="tnpB"
                     /locus_tag="C3F22_RS16140"
                     /old_locus_tag="C3F22_16150"
     CDS             complement(1802..2137)
                     /gene="tnpB"
                     /locus_tag="C3F22_RS16140"
                     /old_locus_tag="C3F22_16150"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="WP_158651691.1"
                     /translation="MIRIDEIWLSTQPLDMRAGMDTAMAQVVRAFGYIKPHCAYLFCNK
                     RGHRMKVLVHDGLGIWLCARRLEQGKFHWAQVHQGESMAISPEQLHALIQGLPWQRIGR
                     QQVVTML"
                     /name="tnpB"
     gene            complement(2134..2517)
                     /locus_tag="C3F22_RS16145"
                     /old_locus_tag="C3F22_16155"
     CDS             complement(2134..2517)
                     /locus_tag="C3F22_RS16145"
                     /old_locus_tag="C3F22_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005100737.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_005232272.1"
                     /translation="MTTNHQTSIASLAKKRRTYSAEFKQQIVQACKAPDVSIASVALQH
                     GLNTNLVSKWIRLIDAKPGNDRSPLPNKPAFIALSCSTPLDPTPTDMLTVQITLPHSKA
                     EIGLKWQVSEISALAELLKALAT"
                     /name="transposase"
     gene            complement(2875..3486)
                     /locus_tag="C3F22_RS16155"
                     /old_locus_tag="C3F22_16165"
     CDS             complement(2875..3486)
                     /locus_tag="C3F22_RS16155"
                     /old_locus_tag="C3F22_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889724.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_005407429.1"
                     /translation="MIKMNTRIYLRASTKDQDAERALQILQDLNQNLNLGETIVYVENY
                     SGTKLDRPELNKLLSEANQGDTLLVESIDRLSRLTQQDFQELKRRIQEKGLRLVVADLP
                     TTYQMIQTSDSITHSILELINNMLIDLLATMARLDNEKRIERIKQGLARSGYKPTGKKA
                     NEAKHKRIKELLAAGNMTKEEIAKAVNCGVATVYRVAKVI"
                     /name="recombinase family protein"
     gene            4630..4938
                     /locus_tag="C3F22_RS16170"
                     /old_locus_tag="C3F22_16180"
     CDS             4630..4938
                     /locus_tag="C3F22_RS16170"
                     /old_locus_tag="C3F22_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019396631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228203597.1"
                     /translation="MSCDEPELEFRFSDQILQKLDEKHGVTIEEVYEAFDNSDGRTLID
                     TRDRHKSHPPTEWFVSQTRKGRALKICYIFDEDKNIIFVKTAYEPNAEEVRIFNKYA"
                     /name="hypothetical protein"
     gene            5039..5419
                     /locus_tag="C3F22_RS16175"
                     /old_locus_tag="C3F22_16185"
     CDS             5039..5419
                     /locus_tag="C3F22_RS16175"
                     /old_locus_tag="C3F22_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_104427042.1"
                     /translation="MKQDKAVNTQDNIFEDVAQWESRNLGADESFVKSKGMSKKLKNLL
                     NNRTSQKMQMISIRLPSTLIDDLKNIGEVEGIGYQALSREILQRFVDAENRKKFNDVVA
                     QKRELQRQVELMEAQLKELKHA"
                     /name="hypothetical protein"
     gene            5531..5815
                     /locus_tag="C3F22_RS16180"
                     /old_locus_tag="C3F22_16190"
     CDS             5531..5815
                     /locus_tag="C3F22_RS16180"
                     /old_locus_tag="C3F22_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_104427043.1"
                     /translation="MLNIENNLIIHEKIDQDVTILKGGYLTLHGQITKNLYIQAGGTAV
                     INGMIVENLYIDPQGSAIVNGMVCKDVLNKGNFSAPGMVCGNIINLSKD"
                     /name="hypothetical protein"
     gene            complement(5963..7126)
                     /locus_tag="C3F22_RS16185"
                     /old_locus_tag="C3F22_16195"
     CDS             complement(5963..7126)
                     /locus_tag="C3F22_RS16185"
                     /old_locus_tag="C3F22_16195"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004994878.1"
                     /GO_function="GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]; GO:0004024 - alcohol dehydrogenase
                     activity, zinc-dependent [Evidence IEA]; GO:0008270 - zinc
                     ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="WP_005104957.1"
                     /translation="MRALTYHGARDVRVESVPDPVIQEPDDVILRVTATAICGSDLHLY
                     RGKIPATEDGDIFGHEFMGIVEEVGPEVTEVKKGDRVIIPFVIACGHCFFCEHELMAAC
                     ENTNTGRGAILNKKQIPPGAALFGFSHLYGGIPGGQAEYVRIPKGNVGPFKVPGSLPDE
                     KVLFLTDILPTAWQAVNNAQVGRGSSVAIYGAGPVGLLAAACARMLGAEQIFMVDHHPY
                     RLSFAHQTYGVIPVNFDEVDAAEFIIQNTTGYRGVDAVIDAVGFEAKGSMLETVMTNLK
                     LEGSSGSALRQCIAAVRRGGVVSVPGVYAGPIHGFLFGDAFDKGLTFKMGQTHVHNYLP
                     QLLEHIENGDLSPDLIITHRMKLEDAAEGYRIFDKKEEDCRKVILTP"
                     /name="zinc-dependent alcohol dehydrogenase"
     gene            complement(7370..7702)
                     /locus_tag="C3F22_RS16190"
                     /old_locus_tag="C3F22_16200"
     CDS             complement(7370..7702)
                     /locus_tag="C3F22_RS16190"
                     /old_locus_tag="C3F22_16200"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032049750.1"
                     /translation="MPKPFLGSADDLAKFLDKHFRLGVKENGSSSFKFLNKGEAYSVLK
                     YVARDVINEEVERLKVEDPKAVARYNPGPSKIRLLDPLKPSTTGYIHIVLKRRYGSGNY
                     CIRPIR"
                     /name="hypothetical protein"
     gene            complement(7787..8782)
                     /locus_tag="C3F22_RS16195"
                     /old_locus_tag="C3F22_16205"
     CDS             complement(7787..8782)
                     /locus_tag="C3F22_RS16195"
                     /old_locus_tag="C3F22_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032049752.1"
                     /translation="MIHYSPMSRYTAQKIVDKVGHGAYFYSHFSVEGEDNLFFPKIDKL
                     IKKLTDKYHLDLTSRQRSYRLNTKKEPIADLIVQKRVNSTIFDFWLLITTPNTHKFNTQ
                     LSQINLKPRLSGQRVAEAENVVWNRENEQQEISVIQDYFRDQEKFKFVLQKPYLKLNFG
                     NGKYVELVRLSHSTKNSKKYASNRKKSEKNYTWTWRYDEPTVHLIEKKYKEIINDLISN
                     PNKSVGIGKWQQLNADLQHYTVFKGNRHQVGRLFTQAVGYHYKKGQSNLRNAEYYQPLT
                     LSYLPRQENYAEDFIQFVILRRLFEETGREFGKENVHEENYNQLINQYLI"
                     /name="hypothetical protein"
     gene            11116..12045
                     /gene="repM"
                     /locus_tag="C3F22_RS16200"
                     /old_locus_tag="C3F22_16210"
     CDS             11116..12045
                     /gene="repM"
                     /locus_tag="C3F22_RS16200"
                     /old_locus_tag="C3F22_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237331.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_104427044.1"
                     /translation="MSKELVVKTNRLNQAFQTLSLSEFHIVQLAIVDARHTGTGLSTDT
                     PLRIDALRYAEVFGTTRQNAYQRMKEAEDSLFNRRFSFFDEDGKLVKSRWIQQVKYLDD
                     EGAIELVFTLAVVQGISKIDGIKDFFTQYLLSQTAQLNSTYSARLYELLIQWRAIGKTP
                     VFELATFREQLGIGVNEYKRMDHFKTRVLDLAISEISEKTDIEATYQQHKKGRSISGFS
                     FSFKQKKSKTKLLENQTISGNLDLFSKKMTDSQRHLFSNKLSELPEMSKYSQGTESYPQ
                     FAVRIAEMLKDSEKLKEFAPMLEKVGYR"
                     /name="repM"
     gene            12114..12659
                     /locus_tag="C3F22_RS16205"
                     /old_locus_tag="C3F22_16215"
     CDS             12114..12659
                     /locus_tag="C3F22_RS16205"
                     /old_locus_tag="C3F22_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_171284765.1"
                     /translation="MKALSVIELAQLYGMNRQSIYKRINKGDLSKNSDGKIDFSEALRV
                     FGEPSHNNKDVTTLQSTSVQKETEVDMLKQQVDILKKQLELAHEREVFQREQLEAKDNQ
                     IEAIQRLLEAPKTNMTTFTDQKPSQDIATDPRSKPEPKHDGLTTPEQTEKKRIPVPEHI
                     EQEPEKRSFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(12849..13052)
                     /locus_tag="C3F22_RS16210"
                     /old_locus_tag="C3F22_16220"
     CDS             complement(12849..13052)
                     /locus_tag="C3F22_RS16210"
                     /old_locus_tag="C3F22_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005407430.1"
                     /translation="MFNFKIFNKVSTEVLTIKNDLQLNSELQLINKYKTSTSEDYRKAI
                     VLIFKERGYTRLEIGQLCQRSI"
                     /name="hypothetical protein"
     Typing          11117..12045
                     /name="rep_cluster_1261"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.57"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cggcccgact acaacaaaca cctgccttag ctgagctaga caccctttgc ggcaaggatt
       61 acaaattaaa aagggatctg caagtttttc agtaagcgtc cgctgaaccc catgcctatt
      121 ttttaatttg agttggattt ccagcgatgt ggtagtaatt cttctatttg ggtcgcttta
      181 tgcgtcggca gccttttcag cacatcactg agataggcat acggatccaa cccattcagc
      241 tttgctgact ggattaaagt catgatattt gccgctcgct gtccactgcg cagcgaacct
      301 gcaaacaacc agttcttacg gcccaatgcc cagggacgca ttgagttctc tgcccagtta
      361 ttgtcaatgg gtagattgcc atcatccaga tagcggctta aggctggcca acgcttcaga
      421 ctgtaattga tggccttggc ggttggagaa ctcgatggca ccgtcagata atgttggttg
      481 agccattcat acagttgttg catcaccggt tggctgtgct gttgtcggta ttcgcggcgg
      541 tgttctgctg tactatcggt ctttttcctg agttctgctt ctatcgcata cagtttctga
      601 atcatcagta atgcctgttc agcgatctga cttttcccag tcacatgtag ctcatggaat
      661 ttacgacgtg catgcgccat gcagcccacc tcaatgacat cgcctgattt aaagcgtgct
      721 ttataaccac tgtaatcatc acagaccaga tggccctgcc agccattcag gaactcttct
      781 gcatgctgac ctgaacggct atcctgaaag tcatagatca ccgcttgaac tggattgtac
      841 tgtgtagtgg cataggccca gacataacct ttcttcggtt ttttctcatc atcacccatc
      901 cgcatgatgg tgaccggcgt ttcatcggca tggatcaccc gctgttgcag caccacctct
      961 tttaaggcat tggccagagg ctccagttct acaccgcagc gacctatcca gtcagataaa
     1021 gtagatctgg acagatcaat gcccgcccgc tgatagatca ggcgctgacg gtacagcggc
     1081 aaatgatcgg catacttcga taccagcaca tggctgagca gttcaggtga agcaatgcct
     1141 ttatcaatca cataggcggg catcgcttgc tgagtcaggg tgtcacactg atcacagacc
     1201 catttgccac gcacatgctg ttccttatag aactgtgccg gtctgaaatg cagtttttca
     1261 ctgatatctt cgccgatacg acgtaactgg cagccacaac tgcattgggt tgatgcaggt
     1321 tcatgctcaa tacggatggt gtgtagatga tccggcagca gtcgacgttt aggtttgttg
     1381 actgtggctt tctctgtcgc tgcatcggtt ttatctgcat tcagccgttc cagttccaga
     1441 tcaacggctg caatatcttc ttcaaccgct tcgtcccaca gatggatttg ttttgcggtg
     1501 agatgttcgt ttttactgcc aaatttgtgc tgtttaaata gtgcgagctc atgctcgtat
     1561 ttttgcgtga gaatggaaag atgttgaact ttggcatcca attgctggtt tgatttttct
     1621 aattcttgat ttgattgtgc cagagactga tgctgcattg ccaactgtct ggtaaattcc
     1681 agcagttgtt catgggtcag ttggcttaag tctggcagcg tattcatgac cgcagtatgc
     1741 ctgaggtcat gacgcagagg aaatagaacg tttggagaat agcagaatgg accagcctag
     1801 tttagagcat cgtcaccacc tgctgtcgtc caatgcgctg ccaaggtaaa ccctggatca
     1861 gtgcatgtaa ctgttccgga ctgatcgcca tgctttcacc ctggtgaacc tgcgcccagt
     1921 ggaattttcc ctgttccagc cgccgggcac acagccagat gcccagtcca tcatgtacca
     1981 gcactttcat gcgatggcca cgtttattac agaacaggta agcacaatgc ggtttgatgt
     2041 agccaaaggc tctcaccacc tgagccatgg ccgtatccat ccctgctcgc atatccagag
     2101 gttgggtaga aagccagatt tcatcaatgc ggatcatgtt gcaagtgcct tgagtaattc
     2161 tgctaaagca gatatttctg atacttgcca tttcaagcca atttctgctt ttgagtgggg
     2221 taaagtaatt tgaaccgtta acatgtcagt aggagtagga tctaatggtg tagagcaaga
     2281 taaggcaata aatgcaggtt tattcggtag tggtgagcga tcattccctg gcttagcatc
     2341 aattaagcga atccatttgg atacgagatt tgtattcaat ccatgttgca aagcgaccga
     2401 agcaattgaa acgtccggtg ctttacaagc ctgaacgatc tgctgtttaa attcagcact
     2461 gtatgttctt cgttttttcg caagagatgc gatggatgtc tggtgatttg tagtcataaa
     2521 ttttagtccc cacttgtttt taagtgggga ctaaattaag gcttatagga cgaattcata
     2581 aggtgtgttc agcggacgct tacttttggg cttagttatt agcgttagtt aaataagtat
     2641 aagtcaattt ttacctaatg gatttgcttt tcaactagtt ccaatttgtt taatgcttgc
     2701 ccttttttat gatcattatt aatctttaca actagagcta aaataaaaac catcactata
     2761 aaaacaagga aaatcaaatg ctttttcatc attaatccct tcttttgatc tttaagaatg
     2821 ccttgttcct ttgtaaatag atatgacata agggaagcac tgatacaatc ctttttaaat
     2881 gactttagca actcgataaa cagttgcaac tccacaattc actgctttgg caatttcttc
     2941 cttagtcata ttgccagctg ctagtaattc ttttattcgt ttgtgcttag cctcattggc
     3001 tttcttacct gttggcttgt aacccgagcg tgccaagccc tgcttaatac gttctatacg
     3061 tttctcattg tccaggcggg ccattgttgc caggagatca atcaacatat tattgatgag
     3121 ttccaggatt gaatgagtaa tgctgtcact ggtttgaatc atttggtagg tagtaggaag
     3181 atccgctacg actaaacgaa gtcccttctc ctggatcctg cgcttcagct cttgaaaatc
     3241 ctgttgggtt aggcgtgata atcggtcaat actttcaact aacaaggtgt caccttgatt
     3301 tgcttctgac agtagcttat tcaattcagg tcggtctaac ttcgtaccac tatagttttc
     3361 cacgtacaca atggtttcgc ccaaattcaa gttttggttt agatcctgaa gaatctgcaa
     3421 agccctttct gcatcttgat ctttagttga agctcgtaga taaatacgtg tattcatttt
     3481 tatcatttaa tcttaattct attagattaa tgataacact atcatttaat tattgcatta
     3541 agtttaatga tagatatttt atgttatttg gctcgttatc attcagctat actcttttga
     3601 taaaaaaagc atgactaaaa gccatgcctt gggaattcgt ataacgtgta ttatgttaat
     3661 tttaagaaca cttcagaact gatctactta ctgataataa atactttgcc taatcgtttt
     3721 agtatttttt cgatagtggg ctctaagact tctggagtta aatttagttt aatttctaca
     3781 ggataagagg ttaataactc tttgatagga ggcctttgtt cataaggcag acactcaatt
     3841 agaatcatta gaactttagg ctcgaagcat ctttttgatg tcgctttgag agttaggcta
     3901 ggcatggacc acctaagtta taatatttta tataagtgcc actgtatcag atatttaaat
     3961 aaagaaagaa tggttctttc tatcaaaaaa agaatcagat gataaatata tgatcgtatt
     4021 ttattcccca tttaacataa tggtgattat acgcaataca cttgtatatt aatataaata
     4081 tcaatgactt aaaaatttcg tcataaccaa gaaaaggcaa aaaaacgact tttgtaataa
     4141 gttggtgagt ttttaagcga atctgtaact tttagccaat aaaatcggtt aaaaaatgct
     4201 cattttcggc atttccccaa tgttcccggt cttcccaggg ccaaaaatat ccccaatttc
     4261 tgtggataaa atttaggcta ttttgtacgc tcaggcttac gtgaatctcg gccctttgcg
     4321 gctatctagg atactggaac tttcctatga tgaactcatc aggaacagga agttccataa
     4381 cataaaacaa aaattataag ttattgcaca tggaccagag ggtacaacag cacaatcagc
     4441 gacaataaaa aaccccggca ggatgccggg gttttttatt tttaaagttt ttaattgtat
     4501 atacattttt tttaatttta tgacttgaat aacataaatt catcaccata taacggtttg
     4561 tgtatgtatt taacaataaa acgtatatac acggatcgtt taaaacttta gaataatgga
     4621 gtacaaaagg tgtcatgtga tgaacccgaa ttagaatttc gcttctctga tcagattcta
     4681 caaaaactgg atgagaagca tggtgtcaca attgaagaag tctatgaggc ttttgacaat
     4741 agtgatggaa gaaccttaat agatacacga gatcggcata aatcacatcc acctacggaa
     4801 tggtttgtta gccaaactcg caaaggcaga gcccttaaaa tttgctatat atttgatgag
     4861 gataagaaca ttatttttgt taagacggca tacgagccta atgcagaaga agttcgaatc
     4921 tttaacaaat atgcttaatg ctccaacgcg ttagtggggg atccccccac ttaactcatc
     4981 tacttattga agtagatgca atctaaatag attgtaaatt aataattaga gtaaagttat
     5041 gaaacaagac aaagctgtaa acactcaaga taacatcttt gaagatgttg ctcagtggga
     5101 gtctagaaat ctaggagccg acgagtcatt tgtgaagtct aaaggcatgt ctaaaaagct
     5161 taaaaatctc ttgaacaata gaacttcaca aaaaatgcag atgatatcta tacgtttacc
     5221 atcgacatta atagatgatt taaaaaatat cggtgaagta gaaggtattg gctatcaagc
     5281 attatctcgt gaaattcttc aaagatttgt agatgccgaa aatagaaaga aatttaatga
     5341 tgttgtagca caaaaaagag agttacaacg tcaagtagaa ctaatggaag cacaacttaa
     5401 agagctaaaa catgcttaaa atatgattct ttgtactcca taaaaataaa cgatcccaga
     5461 acagcaaggt attaccttgc tgttctgtat tatgatataa gcaattgtta attaaggact
     5521 taatagttat atgttaaaca tagaaaataa tttaataatt catgaaaaaa tcgatcaaga
     5581 cgttactatc ttaaaaggcg gctatttaac tcttcatgga caaatcacta aaaacctata
     5641 cattcaagct ggtggtacag ctgttattaa tggaatgatc gtggagaatc tttacattga
     5701 ccctcaaggt agcgcaattg ttaatggaat ggtttgtaag gatgtgctca ataaaggaaa
     5761 tttttcagca ccaggaatgg tctgtggaaa tataattaat ttaagtaaag attaaataat
     5821 ttctttctcc tgaggatcct caatacagag atttgattta acataaaatc tcttatacgc
     5881 aattcagttg taacccccaa atggaaatgt ccgatttctc caaagtaaaa aagcgcctga
     5941 tggcgctttc ctttaatcat atttatggcg tcagaataac tttacgacag tcttcttctt
     6001 ttttatcaaa aatacggtaa ccttccgcag catcttccag tttcatgcga tgggtaataa
     6061 tcagatctgg agacagatct ccattttcaa tatgttctag caactgcggc aagtaattat
     6121 gcacatgagt ttgccccatt ttaaaggtta aacctttgtc aaaggcatca ccaaacagga
     6181 agccatgaat tggaccagca taaactccag gtacactcac cacaccgccg cggcggaccg
     6241 ctgcaataca ttgtcttaac gctgaaccac tcgaaccttc taatttcagg ttagtcatta
     6301 cggtttccag catgctgcct ttggcttcaa aaccgactgc atcaatgacg gcatcgactc
     6361 cacgataacc cgtggtattt tgaatgataa attcagcggc atcgacttca tcaaaattga
     6421 ccggaatcac cccataggtt tgatgtgcga aactcagacg ataaggatgg tgatcgacca
     6481 taaaaatctg ctcggcaccg agcatccgtg cacaggcagc agccagcaga ccaactgggc
     6541 cagcaccata aatcgccaca ctggaacctc gaccaacttg ggcattgttc actgcttgcc
     6601 aggcggttgg tagaatatcg gtcaggaata atactttttc atcaggcagg gagcctggca
     6661 ctttaaatgg tccgacattg cccttgggga tccgcacata ttcagcttga ccacccggta
     6721 taccaccata cagatggcta aagccaaata aagctgcccc tggtggaatc tgttttttat
     6781 tgagaattgc accacggccg gtattggtat tttcacaggc agccattagt tcatgttcac
     6841 agaaaaaaca gtgaccacat gcaatcacga aaggaataat tacccggtcg ccttttttga
     6901 cttctgtgac ttcaggaccg acttcttcta caattcccat aaattcatgg ccgaaaatat
     6961 cgccatcttc agtggccgga attttacccc ggtataggtg cagatctgag ccacagatgg
     7021 cagtcgctgt cacccttaaa atgacgtcat ctggttcctg aatcactgga tctggaacgg
     7081 attcaactcg tacatcccga gcaccatgat aggtaagagc acgcatgcgg atttcctctc
     7141 tctagttcaa cattaaacag actcgtatca aggctgccaa aaataaaaaa cacttttatt
     7201 agagccttat gcgccaagaa atcgctgtaa attaatgtat gaaataagac atccgagtgt
     7261 ttaaaaaata aatttatata gggtttacgt aattcgtgat cttatgggtc gaatgaatag
     7321 aaaagaacag aattaaagaa acacctagat tgttagaacc catttctagc tatcttatag
     7381 gcctaatgca gtagtttccg ctgccatatc ttcgcttaag tactatatgt atataccctg
     7441 ttgtagaagg tttaagagga tccaataatc taattttaga aggtccggga ttgtaacgag
     7501 ctactgcttt cggatcttca acttttaaac gctctacttc ttcattgata acatcacggg
     7561 ccacatattt taaaacgcta taagcctctc ctttattcag aaatttgaaa gaggaacttc
     7621 cattttcctt tactcctaaa cggaagtgct tatctaaaaa tttagctaaa tcatcagcag
     7681 aacctaaaaa aggttttggc ataatcttct ccttaaacta atccttatat tattcagcat
     7741 tagaactgca aactttatta agtatcaatc cattaacagt tttaatttaa ataagatatt
     7801 gattaatcaa ttgattataa ttttcttcat gcacattttc cttaccaaac tctcgtcctg
     7861 tttcttcaaa aagacgtctt aaaatcacga attgaataaa atcttccgca taattttctt
     7921 gcctaggcaa atacgataac gtcaacggct gataatattc agcgttcctc aaattcgatt
     7981 gtcctttctt gtagtgatag ccgacagctt gcgtgaacag tcgcccgact tgatgacgat
     8041 tacctttgaa cacagtgtaa tgttgtaaat cggcatttaa ctgctgccat ttcccgatgc
     8101 ctactgattt attagggtta gaaatcaaat cattaataat ttctttgtat ttcttctcga
     8161 ttaaatgaac ggttggttcg tcataccgcc atgtccaagt gtagtttttc tcactctttt
     8221 tacgatttga agcatatttt tttgagtttt tggtcgagtg tgaaagtcga actagctcga
     8281 catacttacc attgccaaaa ttgagcttta aatagggttt ttgaagtaca aatttgaact
     8341 tttcctgatc tcgaaagtag tcctggatga cgcttatttc ttgttgttca ttctcacgat
     8401 tccaaacaac attttcagcc tcagctactc gttgtccaga tagacgtggt ttcaagttta
     8461 tttggctgag ctgcgtgtta aatttatgag tattaggcgt agtaataaga agccaaaaat
     8521 caaaaatcgt actgtttaca cgtttttgca caatgagatc ggcaataggc tcttttttag
     8581 tgttcagtcg gtatgatctc tgcctcgaag ttaggtctaa gtggtattta tcggttaatt
     8641 tcttaatcag cttatctatt ttagggaaaa aaaggttgtc ttctccctca acagaaaaat
     8701 gagaataaaa ataagcgcca tgcccgacct tatctacgat tttctgagct gtataacgac
     8761 tcattgggct gtagtgaatc attttattgt caccaagcaa tatttataaa attgtcatca
     8821 aacaatatta caaaaacttg attgtaatca agcaatattt ataaaaggtt aaagacttat
     8881 tttataagat ttttataaaa atttagtggc gaagccactt ttgcaaatgc cgaaggcatt
     8941 agccggactt aattgacaca atttgtcatt gtgcaattaa gtggttccta agtgacaata
     9001 tttacacctg tttctacctg ctatatgatg gaattattca ttgactgaag cagtctgacc
     9061 aactgcctga ccatttcgtt tttacgattt tgagcaggcg tcagcctgct taaagccatg
     9121 tttttttaga tagcgcacgt tcctctacta gccattttgg gatgaccgat tgagcgtact
     9181 acctctgaag gggggttcgg tacgatctga tgggcgctgg agtttactct ttcgatccaa
     9241 tccaagctgt gatttttctt tatgactttt cacggggttg ggcgacaagg acacgggagc
     9301 ctataagcaa tcgaaagatt gtgaaatggt cgttaggtta gatatagcct agctagatgg
     9361 ttgtttacat cgaaaaccct gttctagcaa ttaccgtgag aacctgacag gacgttaagg
     9421 ttcgttaata acggttatgt attgcggctc atgcacgttg gaaacaacgg ataggcgcag
     9481 ttcataggca caactgtagg cgtttgaggt aacagcgatg ttatcggctg cggagaggaa
     9541 acttggaagc agcggcacca ctgagtgaga tagtctcaag caaggtgggt caacggatag
     9601 ctcaagcagt atacatcggg atgcatgaaa taattccata attgcccgag atacgtgtag
     9661 atcaagagcc atacgctcag attttttctg atttgccata tcctcatttt tttaaggaac
     9721 cgcgttgggg ggaaactgga agtgcaggtc aggagagaga gcattggcgg tctggtcgtt
     9781 aagattgcta gaaataactt gggaatctct tagctgctgc tgtcatggca ctggtaggtt
     9841 aagagaggga gagacgatga gtagggcgta taaggcatat aacttgttat atgttgaagt
     9901 cgtggcaaat attgctaaaa caataggcga actcaattgc gactggcagt cgtaattggt
     9961 aaagggagtc taaactctat gtgatgttta cgtggagccg tttccgaaag tttgagtcgg
    10021 ttttatgttg ttatctaaaa aagcgaaaaa ctcgttaaag atggtttggt cgccatcgtt
    10081 gttttgcttg ggtcaggatt ggtctcctga cttatgcaag actaaacaac agtctaatga
    10141 aagaatgtga tgtgatattt agccactttt agagtgcaat agtcttgtta atcaccgctc
    10201 tgttcaatag tgccaggtgc tatttctctg gatagtttta aagtttagaa aaactgatgt
    10261 tagcagacat tccaatctgt gagtttttac tcttttttag taaagaaagg gcgtactgtg
    10321 atgattctta ggctctgccg acctaagtta ttcgataaac agcgtggtag ttacgtcagt
    10381 gcactatagc cccctcggtt ttcttcggca aagaaagcga aacgaggtgg gtgtctttaa
    10441 tttctaaaat gcgatatttt gcgattttag gaactttcta acttaggaca tagaatctat
    10501 gcttaaacgt agtaaatgag ctaaaacccc cattttttgc gtttaaaagc cattctcaga
    10561 acgagtgtct acgagtgaac attgaaaagt tgcgcctaga cttcctgaaa aacagttttt
    10621 tcagtatata aatttctaaa aacagaaaat tacgtctcca aagtcgattc cagatcgagc
    10681 gtagcgagca aaaaaagctt atgagcgaag cgaattccga gttgcttttg ctttttctaa
    10741 aagtcacgaa aatattattc aaaaattgcc ccgacgaatc gagcggaagc gagattcaat
    10801 agagtttgag cgtagcgaaa accgagggca atttttcatt ccctgtgttt ttaattattt
    10861 taaggttttt aaatgttttt aacttatttt aaattattaa atttcaataa ctaacctttg
    10921 cattccccca cgtttagtta cccattcccc cacgtttagt tacccattcc cccacgttta
    10981 gttacccatt cccccacgtt tagttaccca ttcccccacg tttagttacc ctcctaatta
    11041 cacccttaac tacacagttg tagttatacc cacattgtta taatctgtgg gtataaaaat
    11101 atttcaatcg agtctatgtc aaaagaatta gttgtaaaaa ccaatcggtt aaatcaagct
    11161 tttcaaactc tatctttatc agagtttcat attgttcaat tagccattgt tgatgctaga
    11221 catacgggta ctggactaag tacagatact cctttacgaa tagatgcttt gagatatgct
    11281 gaagtttttg ggacaacaag gcaaaatgct tatcagagaa tgaaagaagc agaggattct
    11341 ttatttaaca gaagatttag cttctttgat gaagatggga aattagtcaa aagtagatgg
    11401 attcagcaag taaaatatct agatgatgaa ggagctattg aacttgtttt cactttagct
    11461 gttgtacaag gcataagtaa aattgatggt attaaagact tttttactca atatttacta
    11521 agccaaacag cccagcttaa cagtacatat tcagcaagat tatacgagtt attaattcaa
    11581 tggagagcta ttggaaaaac gccagtattt gaattagcaa cttttagaga acaacttgga
    11641 attggtgtta atgaatacaa gcgtatggat cactttaaaa ctagagttct tgacttagcc
    11701 atttctgaaa ttagcgaaaa aacagatata gaggcaacct accaacagca taaaaaaggc
    11761 cgttcaatat ctggtttttc tttttctttt aaacaaaaaa aatctaaaac taaattatta
    11821 gaaaatcaga caatatctgg gaatctagat cttttttcaa aaaaaatgac tgactctcaa
    11881 cgccacttat tttccaataa gctatctgaa cttcctgaaa tgagtaaata ctctcaagga
    11941 actgaaagct atcctcaatt tgcagtaaga attgctgaaa tgcttaaaga ttctgagaag
    12001 ttaaaagagt ttgctccaat gctcgaaaag gttggctatc gttaaatcat tgatgtaacg
    12061 ttacatcact aaatattgtc tactgacata cagactttta cttgggttac atgatgaaag
    12121 cactatctgt tattgaatta gctcaactct atggaatgaa tcgtcaaagt atctataaac
    12181 gtataaataa aggggactta tcaaaaaata gtgatggaaa aatagatttt tcagaagctc
    12241 tacgtgtatt tggtgaacca tcccacaata ataaggatgt aaccacgtta cagtctactt
    12301 cggtacaaaa agagacagaa gtagacatgc ttaagcaaca agtagacata ctaaaaaaac
    12361 agctagaact tgcacacgaa cgagaagttt ttcaacgaga acaactagaa gcaaaagaca
    12421 atcaaataga agcaatacaa cgactgttag aagctccaaa aaccaatatg actacgttta
    12481 ccgatcagaa acctagccaa gatatagcaa cggatcctcg gtcaaaacct gagccgaaac
    12541 atgacggatt gactactccg gaacaaacgg aaaagaaacg gattccagta ccggagcaca
    12601 ttgaacagga accggaaaaa aggagctttt ggagccgttt ttttagaccc tatgattaac
    12661 cacgattgtt taactaatat gaataaagtc gtgaaacttt gattaaataa taagcgtgga
    12721 acgtgaaaaa atcataaaaa aaagcccacc ttggggaggt ggggcaaaaa agaaactaca
    12781 gcgttgaatt ttcaagagaa ttatacagca tttcgtataa ggtgtattat gttaatttta
    12841 ggaaaagttt aaattgatct ttggcatagt tgacctattt ctaacctggt gtaaccacgt
    12901 tctttaaaga ttaaaacaat agcttttcta taatcttcag aagtagacgt tttatattta
    12961 ttgattaatt gcagctcaga attaagctga aggtcatttt ttatcgtcaa tacttcagta
    13021 gagactttat taaaaatctt gaaattaaac atacaggtgc tctttatcaa tattgtagcg
//
