LOCUS       NZ_CP026421            11503 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter sp. ACNIH1 plasmid pACI-3bd5, complete sequence.
ACCESSION   NZ_CP026421
VERSION     NZ_CP026421.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03455971
            Assembly: GCF_002934695.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. ACNIH1
  ORGANISM  Acinetobacter sp. ACNIH1
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 11503)
  AUTHORS   Weingarten,R.A., Johnson,R.C., Conlan,S., Ramsburg,A.M.,
            Dekker,J.P., Lau,A.F., Khil,P., Odom,R.T., Deming,C., Park,M.,
            Thomas,P.J., NISC Comparative Sequencing Program,C., Henderson,D.K.,
            Palmore,T.N., Segre,J.A. and Frank,K.M.
  TITLE     Genomic Analysis of Hospital Plumbing Reveals Diverse Reservoir of
            Bacterial Plasmids Conferring Carbapenem Resistance
  JOURNAL   MBio (2018) In press
REFERENCE   2  (bases 1 to 11503)
  AUTHORS   Segre,J.A. and Mullikin,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2018) NISC, NIH, 5625 Fishers Lane, Rockville, MD
            20852, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 13:28:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,625
            CDSs (total)                      :: 3,512
            Genes (coding)                    :: 3,284
            CDSs (with protein)               :: 3,284
            Genes (RNA)                       :: 113
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 91 of 228
            Pseudo Genes (incomplete)         :: 141 of 228
            Pseudo Genes (internal stop)      :: 76 of 228
            Pseudo Genes (multiple problems)  :: 75 of 228
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP026421.1.
            Bacteria available from Karen Frank, Department of Laboratory
            Medicine, National Institutes of Health Clinical Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11503
                     /organism="Acinetobacter sp. ACNIH1"
                     /mol_type="genomic DNA"
                     /strain="ACNIH1"
                     /db_xref="taxon:1636603"
                     /plasmid="pACI-3bd5"
                     /geo_loc_name="USA"
                     /lat_lon="38.984652 N 77.094709 W"
                     /collection_date="2014"
     gene            join(11231..11503,1..653)
                     /gene="repM"
                     /locus_tag="C3F22_RS16065"
                     /old_locus_tag="C3F22_16075"
                     /pseudo=""
     CDS             join(11231..11503,1..653)
                     /gene="repM"
                     /locus_tag="C3F22_RS16065"
                     /old_locus_tag="C3F22_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004734284.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /name="repM"
     gene            688..1215
                     /locus_tag="C3F22_RS16070"
                     /old_locus_tag="C3F22_16080"
     CDS             688..1215
                     /locus_tag="C3F22_RS16070"
                     /old_locus_tag="C3F22_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004734286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_104427038.1"
                     /translation="MSEVDISKYAALYEYQKNQFSIVKGHYEKLEDKATKYLTYLSIFI
                     TAFSLLAKYYFIDTNIKAPFFLTCLTSLYLALTFIFVCLASGKLFSCLQVKEVFQVNTD
                     DQMIDYFQNNKIATVYLGLAHHYKKVISSYTEKNDEKANLLHKAFDYIQLAGASLVIVI
                     ILIILGKFLGSL"
                     /name="hypothetical protein"
     gene            complement(1245..2453)
                     /locus_tag="C3F22_RS16075"
                     /old_locus_tag="C3F22_16085"
     CDS             complement(1245..2453)
                     /locus_tag="C3F22_RS16075"
                     /old_locus_tag="C3F22_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="WP_031974690.1"
                     /translation="MNEQKLKDLAAEFAKGIKTEDDLNQFTRLLTKLTVETALNAELTE
                     HLGHEKNARKNGSNARNGYSSKTVLSDDGEIEITTPRDRDGTFEPQLIKKNQTRITQMD
                     NQILSLYAKGMTTREIVATFKEMYDADVSPTLISKVTDAVKEQVAEWQNRPLDALYPIV
                     YMDCIVVKVRHNGSVINKAVFLALGINLDGQKELLGMWMAENEGAKFWLNVLTELKNRG
                     LQDILIACVDGLKGFPDAINSVYPQTHIQLCIIHMLRNSLKYVSWKDYKAVTQDLKTVY
                     QSPTEEAALMALDQFAQTWDDKYPQISKSWRTHWENLNTFFAYPAEIRKAIYTTNAIES
                     LNSVIRQAIKKRKVFPTDDSVRKVIYLAIDAASKKWNMPIRDWRLAMSRFIIEFGDRLS
                     NHL"
                     /name="IS256 family transposase"
     gene            complement(2958..3239)
                     /locus_tag="C3F22_RS16080"
                     /old_locus_tag="C3F22_16090"
     CDS             complement(2958..3239)
                     /locus_tag="C3F22_RS16080"
                     /old_locus_tag="C3F22_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005022338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005022338.1"
                     /translation="MTKAAETLEKKIEAQLEKLKQLKARKQAIEARERSKQKEQERKDD
                     TRRKILLGSYLIKKMQENEANKEKILAELNEYLTEERDRKLFNLSEKF"
                     /name="hypothetical protein"
     gene            3454..4407
                     /gene="mobQ"
                     /locus_tag="C3F22_RS16085"
                     /old_locus_tag="C3F22_16095"
     CDS             3454..4407
                     /gene="mobQ"
                     /locus_tag="C3F22_RS16085"
                     /old_locus_tag="C3F22_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_005022340.1"
                     /translation="MAIYHCTTKTVNRSSGRTAVASMAYRAGEKLTDERTGLTHDFTRK
                     EGVVYTEILSNLDTELDRSKVWNLAEKSENRKDARTAREWVIALPDELDEEQRKELARE
                     FAQSLVDRYGVVADLAIHAPSKGGDDKNHHAHILLTTRKAELDTENKLVLTQKSEIELS
                     NTKRKSLGMGTSQEEIKQIRATWANLANHALEYAGYRERIDHRSYADQGNQLQATIHEG
                     SKVTQMRRKGIDTEISRFNDTIKQQNSQRLQYKQQHKEQTLEQGFNRVEKGFEQWKKDQ
                     EAKRLELEHKKQLKLQQEQTMKLKQRKSMNRNGPSL"
                     /name="mobQ"
     gene            4404..4874
                     /locus_tag="C3F22_RS16090"
                     /old_locus_tag="C3F22_16100"
     CDS             4404..4874
                     /locus_tag="C3F22_RS16090"
                     /old_locus_tag="C3F22_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005022342.1"
                     /translation="MSNQNDLDDQLYILLASMKEYREAIADDNKRLETFYNKVASGVLE
                     QSKKTLNNANQEATRALQGRIQELDKATDKLNYRFIALLCAIFLSLVLVFLSFIFLFIP
                     SFDEIKERRAEAAWLEQRYNLDIKNCNGKSCVRIMKNDCHGPNKDYCVIDPK"
                     /name="hypothetical protein"
     gene            complement(4994..5209)
                     /locus_tag="C3F22_RS16095"
                     /old_locus_tag="C3F22_16105"
     CDS             complement(4994..5209)
                     /locus_tag="C3F22_RS16095"
                     /old_locus_tag="C3F22_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005022345.1"
                     /translation="MQNELERYKNFDPTNAPVVKPNFVKKLQERHAIAQAKAFDADVVT
                     WLSSQDDETKQHINDMVRHIMALKRV"
                     /name="hypothetical protein"
     gene            complement(5199..5495)
                     /locus_tag="C3F22_RS16100"
                     /old_locus_tag="C3F22_16110"
     CDS             complement(5199..5495)
                     /locus_tag="C3F22_RS16100"
                     /old_locus_tag="C3F22_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_004282231.1"
                     /translation="MPLIEYYGLLFEWHDKKMEIVYRGREITFEEVCSVFLDPELISFD
                     DLGHYDEQRLISIGLSNRGRLLTVVWVERGDVARIITAFEPSHNQKRRYNHAK"
                     /name="BrnT family toxin"
     gene            complement(5909..7216)
                     /locus_tag="C3F22_RS16105"
                     /old_locus_tag="C3F22_16115"
     CDS             complement(5909..7216)
                     /locus_tag="C3F22_RS16105"
                     /old_locus_tag="C3F22_16115"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011533810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_104427039.1"
                     /translation="MIKMTSDKRHHVVVVGAGFGGLDVVNCLSSADVNITIIDRRNYHL
                     FQPLLYQVAGASLSTTEIAWPIRHLFRNRQNVQTLMAEVQGVDVDARQIILDNGSTVGY
                     DTLVLATGATHAYFGHDDWEPHAPGLKTLEDATTIRGRILCAFEQAERSTSSAERAALQ
                     TFVIIGGGPTGVELAGTIAELAKDTLAHDFRSIDPRTTRVVLIEAGNRLLSVFPETLSE
                     YTRQALEKLGVEVVLGSPVTDCSAEGVIVAGKQLAAKTIVWAAGVQASPAARWLNVKSD
                     RAGRVLVGPDLTVPGHSEIFVIGDTAAAMMPDGKYVPGIAPAAKQQGKYVANLVMQRLK
                     GKSLDEPFMYKHQGNLAIIGRGLAVIDMGHVKLRGAIAWWIWKLAHIYFLIGVRNRLSV
                     ALSWVWNHSVGYRGSRLIMQPSEPAEHPPRKPTSTD"
                     /name="NAD(P)/FAD-dependent oxidoreductase"
     gene            complement(7233..8066)
                     /gene="fghA"
                     /locus_tag="C3F22_RS16110"
                     /old_locus_tag="C3F22_16120"
     CDS             complement(7233..8066)
                     /gene="fghA"
                     /locus_tag="C3F22_RS16110"
                     /old_locus_tag="C3F22_16120"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439570.1"
                     /GO_function="GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA]"
                     /GO_process="GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="WP_086374880.1"
                     /translation="MERIEHHASFEGWQDVFQHESTVLGCMMRFGIYLPPQAEHEKLPV
                     LYWLSGLTCTEQNFITKAAVQRYAAEHGIIVVAPDTSPRGESVADDAAYDLGQGAGFYV
                     DATELPWSDHYQMYSYVVDELPALIEANFSASSRRSISGHSMGGHGALVIALRNPGRYA
                     SVSAFSPIVAPTQVPWGQKALAAYLGEKNLEAWKQYDTVELIRVAQERLPLLVDQGLSD
                     EFLEHQLRPQLLRIACEQVDHPLTLNLRPGHDHSYYFISTYLSEHMAHHAAALNR"
                     /name="fghA"
     gene            complement(8540..8938)
                     /locus_tag="C3F22_RS16120"
                     /old_locus_tag="C3F22_16130"
     CDS             complement(8540..8938)
                     /locus_tag="C3F22_RS16120"
                     /old_locus_tag="C3F22_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009639360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="WP_086374882.1"
                     /translation="MATPVISGNDIFSHVFLGAVDLKKSVAFYDAALGALGIKNLGPFG
                     NDWVLYGRDKPAFMIARPGNGAEPTSNGVTVGFAAATPAEVNAFHAAGLAAGGTDEGQP
                     GPRGHLPGAYAAYLRDPAGNKVCAYTFV"
                     /name="VOC family protein"
     gene            complement(8991..10100)
                     /locus_tag="C3F22_RS16125"
                     /old_locus_tag="C3F22_16135"
     CDS             complement(8991..10100)
                     /locus_tag="C3F22_RS16125"
                     /old_locus_tag="C3F22_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /GO_function="GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA]; GO:0051903 -
                     S-(hydroxymethyl)glutathione dehydrogenase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006113 - fermentation [Evidence IEA];
                     GO:0009404 - toxin metabolic process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="WP_086374883.1"
                     /translation="MKSRAAVAFGPGKPLEIVEIDVEPPRKGEVLVKITHTGVCHTDAF
                     TLSGDDPEGLFPVVLGHEGAGIVVEVGEGVTSVKPGDHVIPLYTAECGECLFCKSGKTN
                     LCVAVRATQGKGLMPDGTTRFSYNGQPLFHYMGCSTFSEYTVVAEVSLAKINPDANPEH
                     VCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGGIGLAAIQGARQAKAGRIIAIDTNPT
                     KFELARSFGATECINPKDYEKPIHEVLIEMTGWGVDHTFECIGNVNVMRSALEAAHRGW
                     GQSIIIGVAGAGKEISTRPFQLVTGRTWKGSAFGGVKGRTQLPGMVEDAMKGEIDLAPF
                     VTHTMGLDKINDAFDLMHEGKSIRTVIHY"
                     /name="S-(hydroxymethyl)glutathione dehydrogenase/class III
                     alcohol dehydrogenase"
     gene            complement(10122..10397)
                     /locus_tag="C3F22_RS16130"
                     /old_locus_tag="C3F22_16140"
     CDS             complement(10122..10397)
                     /locus_tag="C3F22_RS16130"
                     /old_locus_tag="C3F22_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal/formaldehyde-sensitive transcriptional
                     repressor"
                     /protein_id="WP_031983787.1"
                     /translation="MPNQVEDKKKILTRVRRIKGQAEAIEKALESNVECNAILQQICSI
                     RGAINGLMNEMLEVHLKDTLVSGETTEQQRNEELAEIAKILKSYLK"
                     /name="metal/formaldehyde-sensitive transcriptional
                     repressor"
     Typing          4..634
                     /name="rep_cluster_1280"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.506"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          3455..4404
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.283"
                     /coverage="100.0"
                     /evalue="2.59e-177"
ORIGIN
        1 agaatgtaat aagtcgatgg gtacaacgaa tatcttatgt agagaatgaa gctcttgttc
       61 gtattaagtt ttctgatgat gttgtaccgt tgattacaaa tttagaaaaa cacttcacca
      121 gttatgaact agagcaagtc agcagtttaa ctagtgttta cgctatacgc ttgtatgagt
      181 tgcttatcgc atggcgtagt acaggcaaag tgactatggt agagctagaa gagctaagat
      241 taaaactagg catagaaccc aatgaataca agagaatggg gcaatttaaa gaaaaagttt
      301 tacaccttgc cattgatcaa attaataagt acacagatat aaaagcagag tatgaacaac
      361 acaaacgtgg tcgttcaatt attggttttt catttaagtt taagcagaaa caacaaccga
      421 aaaaattaga ttctaagcgt gacccaaaca cacctgactt ttttatcaga atgaccgatg
      481 cacaacgcca tttatttgcc aataaaatgt cagaaatgcc tgaaatgggg aaatattcac
      541 aaggtacaga aagctaccaa caatttgcta tccgtatagc agatatgctg ttagagcctg
      601 aaaaattcag agagctttat ccaatattag aaaagtctgg attcaaccca tgagtaaaag
      661 agcaaatagc ataatgaaag aggaagtatg agcgaagtag atataagcaa atatgcagct
      721 ttatatgaat accaaaaaaa tcaattttcg atagtaaaag gacactatga aaaattagaa
      781 gataaagcta ctaagtattt aacatattta tctattttta ttactgcctt ttctttatta
      841 gcaaaatact atttcataga tacaaatatt aaggctccat tttttttaac ttgtttgacc
      901 tcactttatt tggctttaac ttttattttt gtatgtttgg catcaggaaa gcttttttct
      961 tgtctacagg taaaagaagt ttttcaagta aatactgatg atcaaatgat tgattatttt
     1021 cagaataata agattgctac tgtttacctt ggtttagctc atcactataa aaaagttatc
     1081 agttcatata ctgaaaaaaa tgatgaaaaa gcaaacttat tacataaagc ttttgactat
     1141 attcaactag ctggagctag tttagttatt gtgataatac tgattatatt aggaaaattt
     1201 ttagggagcc tgtaaataat tttgtgtaat tgcttttcat aaatttaaag gtgattgctt
     1261 aagcggtcac cgaattcaat aataaagcgg ctcatggcta aacgccagtc gcgaataggc
     1321 atattccact ttttagacgc tgcatcaatt gccaggtaaa tcactttacg tacagaatca
     1381 tccgttggaa agactttacg ctttttgatc gcctgacgta ttacgctgtt taatgattcg
     1441 atcgcattgg tggtatagat ggctttgcgt atctcggctg gataagcaaa gaaggtattt
     1501 agattctccc agtgtgtacg ccagcttttg ctgatctgtg ggtacttgtc atcccatgtt
     1561 tgggcgaatt gatccaaggc catcaaggct gcctcttcag taggtgactg ataaacggtt
     1621 tttaaatcct gagtgacggc cttgtaatct ttccatgata cgtatttcaa gctgttacgc
     1681 agcatatgga taatgcacag ctggatatgg gtttgcgggt atacgctgtt aatggcatca
     1741 ggaaagcctt ttagtccatc cacacaggcg atcagaatat cctgcaaccc tcggttttta
     1801 agctcagtca ggacattcag ccagaacttt gcaccttcat tctcagccat ccacatgccg
     1861 agcagttctt tctgtccatc aagattaatg cctaaggcaa ggaatacagc cttgttgata
     1921 acactgccat tatgacggac tttaactaca atacagtcca tatagacgat gggatagagt
     1981 gcatccagcg gacggttttg ccattcagcg acttgctcct taacagcatc agtcaccttg
     2041 gaaataagcg ttggcgatac atcagcatcg tacatttctt tgaaagtagc cacgatttca
     2101 cgggtagtca tgcctttggc ataaagggat agaatttggt tatccatttg cgtgatacga
     2161 gtctgattct ttttaattag ctgtggctca aatgtgccat cacgatctcg tggtgtggtg
     2221 atctcaatct caccatcatc gctcagcacg gtcttactgg aataaccgtt acgagcattt
     2281 gaaccgttct tacgagcatt cttttcatgt ccgagatgtt cggtaagttc ggcattgaga
     2341 gccgtttcaa cagtgagttt agtcaacaat cgggtgaatt gattgagatc gtcttctgtt
     2401 ttgattcctt tagcaaattc tgctgcaagg tcttttagtt tttgttcgtt catcatgttg
     2461 cctgactccg ttgtgaatat gaacatagca aattcaggca attacacaat ttaatttaca
     2521 gtctcttttt aggataacgt aaatgagtaa tccagagcct aaacctgagt atgttccgcc
     2581 accaccacct gttcgtgatc aacttacaac agacttacca aaacctaagt gattcaaaat
     2641 ttcaaaatta gaggtaaaaa tggctgaagc taaacccgaa tatgttccac caccaccacc
     2701 tgttcgtgat caatttacgg cagataaacc caaaccaaag tgattgattt aaaatttcaa
     2761 aatagaggta aaaatggctg aagctaaacc cgaatatgtt ccaccaccac cacctgttcg
     2821 tgatcagaaa ggactgaagt aatgtctaaa aatactgaac ctaaacctga gtatgttccg
     2881 ccaccaccac taataaaaat aggtgattca aaaggtaata aaaagtaact tccaagtaga
     2941 cagtgaaatc tgtctactta aaatttctca cttaaattga ataactttct gtctctttct
     3001 tcagttaaat attcattaag ttcagccaat attttttctt tattggcttc attttcctgc
     3061 attttcttaa tcaagtaaga accgagcaaa attttacgtc gagtatcatc cttcctttcc
     3121 tgctcttttt gctttgacct ttctctagct tcgatagctt gtttgcgagc ctttaactgt
     3181 ttcagctttt ctaactgggc ttcgattttc ttttcgagag tttcagcagc ttttgtcatg
     3241 attcagattt cgggatatag aaaaccactc aagcataagg ccctatgaat tgaaattcaa
     3301 gcgggctata tgctatcgtg tttttcatga aatgcgcact tacgacttgg atttcatcca
     3361 agtcaaagtt tgcgtaaggg gatacccctt aaccccaaaa aattgctacg caatttttta
     3421 aaagcaaaag caaaaccatc acaaggctaa ggcatggcaa tttatcactg tacgaccaaa
     3481 accgttaacc gaagttcggg acgaactgcg gttgcctcta tggcttatcg tgcaggagaa
     3541 aaattgacag atgaacggac agggttaacg cacgacttca cgagaaaaga aggtgtggtg
     3601 tatacagaga ttctttctaa cttggatact gaattagacc gaagcaaagt ttggaatctg
     3661 gcggaaaaat cagaaaatcg gaaagatgcc cgaaccgcac gtgaatgggt aattgctcta
     3721 ccggacgagc tggatgaaga acagcgaaaa gaactagcaa gagagtttgc tcaatcttta
     3781 gtagaccgtt atggggtggt tgcagattta gcgattcatg cacccagcaa aggtggagat
     3841 gataaaaacc atcatgcaca tatcttactg acaactcgca aagcagaatt agatactgaa
     3901 aataaactgg tcctcactca aaaatcggaa attgagttaa gtaacaccaa aagaaaaagc
     3961 ttaggcatgg gaacaagcca ggaagaaatt aaacagattc gagccacctg ggcaaactta
     4021 gccaatcatg ctctggaata tgcagggtat cgggaaagaa tcgaccaccg cagctatgcc
     4081 gatcaaggta atcagctaca agccaccata catgaaggca gtaaagtcac ccagatgcgc
     4141 agaaagggca tagatactga aattagccgt ttcaacgaca cgatcaaaca gcagaacagt
     4201 caacggctcc aatacaaaca acagcacaag gaacagacct tagaacaggg tttcaaccgt
     4261 gttgaaaaag gctttgagca atggaagaaa gatcaggaag ctaaacgcct agaactggaa
     4321 cataaaaagc agttaaagct acagcaagaa caaacgatga agctgaaaca acgcaaaagc
     4381 atgaatagaa atgggccttc cctatgagca atcaaaacga tttagacgac cagttatata
     4441 ttttattggc atccatgaag gaatacaggg aagccatagc agatgataac aagcgattag
     4501 agacgtttta caataaggta gctagtgggg tactagaaca gagcaaaaag accctaaata
     4561 acgcaaatca ggaagctaca cgcgcccttc aaggtcgtat tcaggagtta gataaagcca
     4621 ccgataaatt gaactacaga tttattgcct tgttgtgtgc aattttcctt agtctggtat
     4681 tagttttcct gtcttttata tttttattta ttccaagttt tgacgagatc aaagaacgtc
     4741 gtgcagaagc agcttggcta gaacaaagat ataatctaga tattaaaaac tgtaatggta
     4801 aatcctgtgt ccgaattatg aaaaatgact gtcacgggcc aaacaaagat tattgcgtga
     4861 ttgaccctaa ataaatacgg gtaagtactt tgctcggttc ttctcttaat tttattgata
     4921 ccggatttcg caaagtggct cgactttgaa acatggcaaa agccatattt caaagcatcg
     4981 agctaaattt taattaaaca cgtttcaacg ccatgatatg gcgcaccata tcgttaatgt
     5041 gctgttttgt ttcatcatct tgagaactta gccaagttac aacatctgca tcaaaagcct
     5101 ttgcttgagc aatagcatgg cgttcttgta attttttaac aaaattcggt tttactactg
     5161 gcgcattggt agggtcaaaa tttttatagc gttctaattc attttgcatg gttatacctc
     5221 cgcttttgat tgtgcgatgg ctcaaaggca gtaatgattc ttgcaacatc accacgttca
     5281 acccaaacca ctgttagtag tctaccacga ttacttagac cgattgatat tagccgttgc
     5341 tcatcgtaat gaccaagatc gtcaaaggaa atcagctctg gatcaagaaa aacgctacat
     5401 acttcttcaa aagtaatttc acgccctcga tagactattt ccattttttt atcgtgccat
     5461 tcaaatagca gaccgtaata ctcaatcaac ggcatgttta gcctttttcc tagatttaca
     5521 acctctaaaa gtctacttag agggttttag ttattcaata attcattaat ttaaataggt
     5581 tctaacatcc aaatttcgta taacagccat tatgttaaat agaatgtaaa ttaagctaaa
     5641 actgtgtttt taatgtagac aaactaatgg agtgagatag ctcgaacact ttgaaagatt
     5701 attggtcttt taaatgttta tgaaacaaag ccactttcga agcggacatc atcgaaaacg
     5761 atacgctcag catgtcaggt tggatgggct tcctgcccac tgcagtaagc tccaccatca
     5821 ctctcatagc agccttgctg cagagatcct cgcaaaaagc tgaaccagga gattgttcac
     5881 ttgtagagaa caacagcctc cctgatcgtc aatccgtcga ggttggcttg cgaggggggt
     5941 gctccgcggg ttcactgggc tgcatgatta gacgtgagcc acgataaccg acactgtggt
     6001 tccataccca actcagtgca acgcttaggc gattacgcac accaatcaaa aagtagatgt
     6061 gcgccagctt ccatatccac caggcgatcg ccccgcgcaa cttgacgtgc cccatatcga
     6121 tgaccgccag gccgcgaccg atgatggcga ggttgccctg atgtttgtac atgaacggct
     6181 catcaaggct tttgcccttc agtcgctgca ttaccagatt ggccacgtac ttgccttgct
     6241 gcttagctgc aggtgcgata cctggcacat acttcccatc aggcatcatc gctgctgcgg
     6301 tgtctccgat cacgaagatc tcggaatgac ccggcacggt aaggtccggc ccgaccagca
     6361 cacgccccgc gcgatcagac ttaacgttta accaacgggc tgccggtgaa gcctgcaccc
     6421 cagctgccca gacaatggtt ttagccgcaa gctgtttgcc agcgactatt acaccctccg
     6481 cggagcaatc ggttacagga gaccccagta ccacttccac tccgagtttt tcaagcgctt
     6541 gcctggtata ttccgaaagg gtctcgggaa atacagaaag caagcgattg cctgcctcaa
     6601 tcagtacaac gcgtgtggta cggggatcga tggagcgaaa atcatgcgcc aacgtatcct
     6661 tcgccagttc agcgatggtg ccagccaact cgacacccgt agggccacca ccaatgatca
     6721 cgaatgtctg cagtgcggca cgttccgccg agctagtgga acgctcagcc tgctcgaacg
     6781 cgcaaagaat cctccctcga attgtggtgg cgtcctccag agtctttagg ccaggagcat
     6841 ggggctccca atcgtcgtga ccaaagtagg catgcgtcgc ccctgtcgca agaacaagcg
     6901 tgtcgtaacc gacggttgat ccgttatcga gaatgatttg ccgcgcgtcg acatcgacac
     6961 cctgcacttc cgccattagc gtctgcacat tctgacggtt gcggaacaag tggcgaatcg
     7021 gccaagcgat ttcagttgtc gagagcgatg cgccggccac ctggtaaagc aagggctgga
     7081 ataggtgata gttgcgtcga tcgatgatcg ttatattgac atcagcgctg gataagcaat
     7141 tgacaacgtc taacccaccg aagcccgcgc cgacaacaac aacatgatgc cgtttgtcag
     7201 aggtcatttt tatcattagg gtctcccaat gttcagcgat tcaatgcagc cgcatggtgg
     7261 gccatgtgtt cgctcaagta ggtcgaaata aagtagtagc tatggtcatg accaggccgc
     7321 agattgagcg ttagcgggtg gtctacctgt tcacacgcga tacgcaacag ttgcggtcga
     7381 agctggtgct cgagaaattc atcgctcagc ccttgatcga caagcagcgg taagcgttct
     7441 tgcgccacgc ggatcagctc gaccgtgtca tattgcttcc aggcttccag attcttttcg
     7501 cccaagtaag ccgccagagc cttctgcccc catggaactt gcgtaggcgc cacgatcggc
     7561 gagaacgctg agacacttgc atagcgaccg gggttgcgaa gcgcaataac cagagcgcca
     7621 tgaccgccca tggagtgccc actgatgctg cgtcttgacg acgcagagaa gttcgcttcg
     7681 atcagcgctg gtaattcgtc gaccacatag gaatacatct gatagtgatc gctccaaggt
     7741 agctctgtgg cgtctacata gaaacctgca ccttgaccta gatcataggc agcatcgtcg
     7801 gcaacgcttt ctccgcgcgg gctggtatct ggggctacga caatgattcc gtgctccgcg
     7861 gcataccgct gcaccgcagc cttggtgatg aagttctgtt cggtacaggt cagacctgag
     7921 agccagtaca gaaccggaag cttttcatgc tcagcctgag gcggaagata gattccaaac
     7981 ctcatcatgc agccaagcac tgttgactca tgctgaaaaa cgtcctgcca accctcgaag
     8041 ctggcgtgat gttcaatgcg ttccacggat tttctcctta tttcaacgag gtggtgctca
     8101 ggcgccaatc aagacgaatt ttcgttccat tcaggattag ttaaaaatgc ttcacagcac
     8161 cactcagcag gtttccagcg ttgtcgctgg catgaggatc gctgctacaa acccagacgc
     8221 caccacacca cgaaggttgg atgtcgactt caatgtacaa gcaataaagt gtgtaattca
     8281 gggagccact accgcgatgc tcatttcgaa ttctgtgttt cttgcatcac gcaccgaact
     8341 ttcagtccga tcgtgctcat tgaccgtctt tggccatgaa ggcgaggtaa cagcctttct
     8401 gagctctaat cagcagatag aggctcagag taccgtaaac cgtgcggcat gagcgggcat
     8461 gccacacggt tgcagtggaa gatgagattg tttttggctg gaaccagccc ttacacaatg
     8521 acctcacttc tcctaggttt tagacaaagg tgtaggcgca gaccttatta ccagccggat
     8581 cacgcaggta ggccgcgtaa gcacccggca ggtggccacg tgggccaggc tgaccttcat
     8641 cggtgccgcc agcggcgagg ccggctgcgt ggaatgcatt tacctctgca ggggtggcag
     8701 cggcgaagcc taccgtcaca ccattgctgg tgggttctgc gccattgcct gggcgggcga
     8761 tcatgaaagc aggtttgtcg cgaccgtaca gaacccaatc gttaccgaat ggccctaggt
     8821 ttttaatgcc gagggcacct agagcggcat cgtagaatgc aaccgacttc ttaaggtcga
     8881 ctgcgccgag gaagacatgg gagaaaatat cgttaccgga aattactgga gtagccatta
     8941 aagtgtttcc ttgatggttg gttttgaaaa attaggtatt gatccgtgga tcaatagtga
     9001 atgacagtgc gaatcgactt accttcgtgc atcagatcga acgcgtcgtt gattttatcg
     9061 agtcccatgg tgtgggtgac gaatggagcg aggtcgattt cacctttcat cgcgtcttct
     9121 accatgcctg gaagttgggt acgtcccttg acaccgccga aggcagagcc cttccaggtc
     9181 cggccagtga ctaattggaa cggacgggta gagatttcct taccggcgcc agcaacgccg
     9241 atgatgatcg attgacccca gcctcggtgg gctgcttcca gcgccgaacg catcacattg
     9301 acgttgccaa tgcactcgaa ggtatggtcg acaccccagc cagtcatctc gataagaacc
     9361 tcgtgaatcg gcttctcgta gtccttaggg ttgatgcact cggtggcacc aaaactgcgt
     9421 gccagttcga acttggtcgg gttggtatcg atggcgatga tgcggccagc cttggcttgg
     9481 cgtgcaccct gaatcgcagc aagaccgata ccgccgaggc cgaacactgc caccgagtca
     9541 ccaggctgca ccttagccgt gttgtgcacg gcaccgatac cggtggtcac gccacaaccg
     9601 agcaggcaga catgttccgg attggcgtcc gggttgatct tggccagaga tacctctgca
     9661 acgacggtgt attcgctgaa ggtcgagcaa cccatgtagt ggaacagtgg ctgaccgttg
     9721 tatgagaaac gggtcgtgcc gtcgggcatc aggcccttgc cctgggtagc gcgcactgcc
     9781 acacaaaggt tggtttttcc tgacttgcaa aacaggcact cgccacattc ggcggtgtat
     9841 agtggaatga catgatcgcc aggtttcacg ctagttacac cttcaccaac ttcgacgacg
     9901 atgcctgcgc cttcatggcc cagaacgacc gggaagaggc cttcgggatc gtcaccagag
     9961 agggtgaatg catcggtatg gcaaacgcca gtgtgggtga tcttgaccag cacctcgccc
    10021 ttgcgcggcg gctcgacgtc aatttcgact atttccagag gttttcctgg cccgaaggct
    10081 acagctgcac gtgatttcat ggaatttctc cttacacaaa tttattttaa atatgacttg
    10141 agaatcttgg ctatttcagc aagttcttcg tttcgttgtt gttctgtagt ttcgccagat
    10201 actagagtgt cttttaagtg aacctctagc atctcattca ttagcccatt aatggcaccg
    10261 cgtatagaac atatttgttg cagaatggca ttacattcca cattgctttc tagcgctttt
    10321 tcaatcgctt cagcctgacc tttgattcgt ctgacacgcg tcagaatctt ttttttatct
    10381 tcgacttgat ttggcataaa gctaattcct taaaagtttc ttatagtata gtaccctata
    10441 gtatgttggt tggcaagaag cctttataaa cttaataatt gataaaagct tataatcaaa
    10501 aaattctata tttattttta aatttttcga aaattaacat aatacacctt atacgaaatg
    10561 ctctaaatta tttattataa ttcatagtct tattgtgtat caaaaagccc aagtatgcgt
    10621 acttgggctt ttttaatgat ttgtcacgtt ccacgcctat tatttaagca atgtttcaca
    10681 actttgatta gctccagtaa ataattaaaa cctagataat aagaaccgag cgagtgtcta
    10741 cgagcgattg agcatcatca aaatttcgcc ctcgcctttt ctctgattac aagctccctt
    10801 tcgattttaa acggtaggta cagagcatcc cgaatgggtg cgaactttgg agcttttgct
    10861 tttcaaaaag tatgagcgaa gcgaatacat taccaatgct tttgctttta gatttcacaa
    10921 acaggattaa ccaaaaactg ccccacgaag cgaggaacga gcttcaatag agtacgagct
    10981 ttagcgagta catagggcag tttttagcac tcccctcttt ttaataattt ttaatttttt
    11041 aatttttagc atctgtaatt aattgataaa taataaaaaa aataagaata agtacacctt
    11101 tatcttgcaa taagtacacc tttatcttgc aataagtaca cctctatctt gcaataagta
    11161 cacctctatc ttgcaaagtg acacttaata aaataaagtg tacttatata cttaaaaaac
    11221 atgtgtgctt atgaaaaaag aactagtcgt taaagataat gcactaataa atgccagtta
    11281 taatttagac ctatcagaac aacgtttaat actgttagcc atacttgaag ctaggcaatc
    11341 aaatacaccc aatgataaag atttgacgat tcatgctgaa agctatatca accatttcaa
    11401 tgttcataga aatacagcct ataaagttct taaagatgct tgcaaaagcc tatttgatcg
    11461 tagatttagc tatcaaaaac tgactcaaaa gggcaacatt gag
//
