LOCUS       NZ_CP026393             6761 bp    DNA     circular CON 25-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KPNIH48 plasmid pKPN-8171, complete
            sequence.
ACCESSION   NZ_CP026393
VERSION     NZ_CP026393.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06040387
            Assembly: GCF_002935085.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Weingarten,R.A., Johnson,R.C., Conlan,S., Ramsburg,A.M.,
            Dekker,J.P., Lau,A.F., Khil,P., Odom,R.T., Deming,C., Park,M.,
            Thomas,P.J., NISC Comparative Sequencing Program,C., Henderson,D.K.,
            Palmore,T.N., Segre,J.A. and Frank,K.M.
  TITLE     Genomic Analysis of Hospital Plumbing Reveals Diverse Reservoir of
            Bacterial Plasmids Conferring Carbapenem Resistance
  JOURNAL   MBio (2018) In press
REFERENCE   2  (bases 1 to 6761)
  AUTHORS   Segre,J.A. and Mullikin,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2018) NISC, NIH, 5625 Fishers Lane, Rockville, MD
            20852, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 226x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/25/2023 04:45:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,373
            CDSs (total)                      :: 6,248
            Genes (coding)                    :: 6,055
            CDSs (with protein)               :: 6,055
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 193
            CDSs (without protein)            :: 193
            Pseudo Genes (ambiguous residues) :: 0 of 193
            Pseudo Genes (frameshifted)       :: 73 of 193
            Pseudo Genes (incomplete)         :: 123 of 193
            Pseudo Genes (internal stop)      :: 24 of 193
            Pseudo Genes (multiple problems)  :: 24 of 193
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP026393.1.
            Bacteria available from Karen Frank, Department of Laboratory
            Medicine, National Institutes of Health Clinical Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPNIH48"
                     /db_xref="taxon:573"
                     /plasmid="pKPN-8171"
                     /geo_loc_name="USA"
                     /lat_lon="38.984652 N 77.094709 W"
                     /collection_date="2016"
     gene            join(6247..6761,1..754)
                     /locus_tag="C3F39_RS28420"
                     /old_locus_tag="C3F39_28420"
     CDS             join(6247..6761,1..754)
                     /locus_tag="C3F39_RS28420"
                     /old_locus_tag="C3F39_28420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011191342.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISApu1 family transposase"
                     /protein_id="WP_011191342.1"
                     /translation="MTIINANYYLEQLLAPAALERIARLCKFCLRQRAITPAMLVPALL
                     RAMGGDQVNDIAGLHRHFNALQLVNTHQVSYKPFHNQLRKESFALFMKALVERAIALRI
                     DHQLKAASLGAFKQVLLHDGTSFAVHRRLAADFPGRFKTISPAAIECHMTMSLSEQSPL
                     CMSVSADTAGERQFLPEAHTLTNCLLLADAGYIDRAWFEQVNDAGGFYLVRGTKSLNPK
                     IIQAWRGDGREVPKLAGLSLREAGRRRCRAEVLDMVVKSGQVEYRLIRRWFAEEKRFCL
                     WMTNLPRAAWSAEQVMSLYRCRWQVELLFKEWKSHNRLKGFVTGEKAIAEGLVWTSLLS
                     LVMKRRVAQSVMSGALSMLKASKNSATWWLPLLEAVAHRALTEIRERLEWAADYLAKNA
                     CRTKQRKSIQNRTLEGVLNGLAA"
                     /name="IS4-like element ISApu1 family transposase"
     gene            787..903
                     /locus_tag="C3F39_RS28425"
                     /old_locus_tag="C3F39_28425"
     CDS             787..903
                     /locus_tag="C3F39_RS28425"
                     /old_locus_tag="C3F39_28425"
                     /inference="COORDINATES: protein motif:HMM:NF014457.3"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_072072242.1"
                     /translation="MVLAACQVNNVRDTGGGAVSPSSTVTGVTLDSGTNGNP"
                     /name="colicin release lysis protein"
     gene            complement(1061..1222)
                     /locus_tag="C3F39_RS35050"
                     /old_locus_tag="C3F39_28430"
     CDS             complement(1061..1222)
                     /locus_tag="C3F39_RS35050"
                     /old_locus_tag="C3F39_28430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049165871.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPERHDKAPLAAATGNGFRGVGLE
                     VMRLLRLN"
                     /name="hypothetical protein"
     gene            1627..1818
                     /locus_tag="C3F39_RS28440"
                     /old_locus_tag="C3F39_28440"
     CDS             1627..1818
                     /locus_tag="C3F39_RS28440"
                     /old_locus_tag="C3F39_28440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1818..2240
                     /locus_tag="C3F39_RS28445"
                     /old_locus_tag="C3F39_28445"
     CDS             1818..2240
                     /locus_tag="C3F39_RS28445"
                     /old_locus_tag="C3F39_28445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714834.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2988..>3236)
                     /locus_tag="C3F39_RS28455"
                     /old_locus_tag="C3F39_28455"
                     /pseudo=""
     CDS             complement(2988..>3236)
                     /locus_tag="C3F39_RS28455"
                     /old_locus_tag="C3F39_28455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178052.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            <3235..3717
                     /locus_tag="C3F39_RS28460"
                     /old_locus_tag="C3F39_28460"
                     /pseudo=""
     CDS             <3235..3717
                     /locus_tag="C3F39_RS28460"
                     /old_locus_tag="C3F39_28460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /name="S-type pyocin domain-containing protein"
     gene            3719..3976
                     /locus_tag="C3F39_RS28465"
                     /old_locus_tag="C3F39_28465"
     CDS             3719..3976
                     /locus_tag="C3F39_RS28465"
                     /old_locus_tag="C3F39_28465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_025714833.1"
                     /translation="MAGNKISDFTEEEFTAFVSKIIAADFPTDKENDDAIYSFAQLTEH
                     PAGWNLIFKPKVGEDSSAKGIVKTVKEWRAANGKPGFKKA"
                     /name="bacteriocin immunity protein"
     gene            complement(4127..5401)
                     /locus_tag="C3F39_RS28470"
                     /old_locus_tag="C3F39_28470"
     CDS             complement(4127..5401)
                     /locus_tag="C3F39_RS28470"
                     /old_locus_tag="C3F39_28470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011222061.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISApu2 family transposase"
                     /protein_id="WP_011191341.1"
                     /translation="MTIINATQYLKQLLSSSELNRIGKFTGFCQRLRDIQPARLLPALL
                     SGLGCDKVDGIAGLHRHFNALQLHDTDQIAYKPFHNQLRKQGFPLFMRALVERAIALRL
                     KECLPDAHGLAGFEQVLLQDGSSFALHPQLAEHFPGRFNKHSPAAVECHMTMSLLDQSP
                     ISMSITADTESERKHLPAANSLNNKLLLADAGYISREYFADVTKAKGSYLVRGSQNLNP
                     KVQAAYRQDGREMPKLLGKKLKDIDRRSCRAEVLDLDVSSGKYKYRLIRRWFAEEKRFC
                     IWMTNLPRERWPAEKVMALYRCRWQIELLFKELKSHNRLKAFATRQKAIAEGLIWASLL
                     ALLVKRRLALTLIGSAELSMLKIAKNSALWLMPILASIIHGAWQEITEKLEWAMVYLSK
                     NGKKSRQRKSKQNNTLDGIINSLAA"
                     /name="IS4-like element ISApu2 family transposase"
     gene            5558..5929
                     /locus_tag="C3F39_RS28475"
                     /old_locus_tag="C3F39_28475"
     CDS             5558..5929
                     /locus_tag="C3F39_RS28475"
                     /old_locus_tag="C3F39_28475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019838990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004099020.1"
                     /translation="MTQERIQTRYPLGGPFATQAELEQAITQAITTHRQDFFPLLERLV
                     DVGDTRVNLSQENPLRVLSIELADGREGVAHINYESNFAESCLIINEYDQHQTSLAFRL
                     DGDALIFDVELPIAWNFNN"
                     /name="hypothetical protein"
     Typing          complement(1110..1579)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.834"
                     /coverage="92.0"
                     /evalue="2.73e-170"
ORIGIN
        1 aggggaacgg caatttctgc ctgaagccca cacgctgacc aactgcctgc tgctggctga
       61 cgcaggttac attgacaggg cttggtttga gcaggtgaac gacgctggcg gattttatct
      121 ggtgcgcggc acgaaaagcc tgaaccccaa gataatccaa gcctggcgcg gtgatggacg
      181 ggaagtaccc aagctggcgg gactatctct gagagaggcc ggacggcgac gctgtcgggc
      241 cgaggtactg gatatggtgg tgaagtccgg ccaggtcgag taccggttga tccggcgctg
      301 gtttgctgag gaaaagcggt tctgtctgtg gatgacgaac ctaccgcgag cagcatggtc
      361 agcagagcag gtgatgagcc tgtatcgctg ccgttggcag gtggagttgt tatttaaaga
      421 gtggaaatcg cacaaccggc tcaaggggtt tgtgacgggc gagaaagcga ttgccgaggg
      481 gttggtgtgg acgagcctgc tgtcgctggt gatgaagaga cgggtcgcgc agagtgtgat
      541 gagtggggcg ctatcgatgc tgaaagcgtc gaagaacagt gcgacgtggt ggctaccgct
      601 attggaagca gtggcccata gggcgctgac agaaataagg gaaagattgg aatgggctgc
      661 cgattatttg gccaagaacg cctgccgaac caagcagaga aagtcgatac agaacaggac
      721 cttggaaggc gttttaaatg gactcgccgc ttaaggtcct gtacatgagg ggttgttacc
      781 gtttctgtgg ttctggcagc atgtcaggtc aataatgttc gagacaccgg aggtggggct
      841 gtttcacctt catcgacggt gaccggagtc acgcttgata gcggtacgaa cggcaatccg
      901 taaaggatct tcttgagatc ccatttggat cgtcgtaatc tcttgctctg taaacgaaaa
      961 aaccgccttg cagggcggtt tttccgaagg ttcaaacact tgcaggtgat tgaaccaagg
     1021 taactggctt ggaggagcgc agtcactaaa acttgttctt tcagtttagc cttaacaggc
     1081 gcatgacttc aagaccaact cctctaaatc cgttaccagt ggctgctgcc agtggcgctt
     1141 tgtcgtgtct ttccgggttg gactcaagat gatagttacc ggataagacg cagcagtcgg
     1201 actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga
     1261 gtgtcaggcg tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga
     1321 atggcaggaa caggagagcg cacgagggag ccatcagggg gaaacgcctg gtatctttat
     1381 agtcctgtcg ggtttcgcca ccactgattt gagcgtcaaa ttctgtgatg cttgtcaggg
     1441 gggcggagcc tatggaaaaa cggccgctgt gctgccttct ccctctccga gctgtctgct
     1501 ctatgtcgtt atgtgcgcgt tgcgaccgct cgccgcagcc gaacggcagg agcgtagcga
     1561 ctgagtgagc gaggaagcgg aaaagagatc tgtctgacat tgagcactga cgctctgagg
     1621 ccctttatga cgaaacaaca gaaaacagcg ctgaacatgg ctaaattcat ccagaatcag
     1681 tctctgctcc tgctggagaa gcttaacgag cttgatctcg atgttgaggc cgacctttgt
     1741 gagaaactcc acgatgatgc ggagcatctt ttccgtacac tgtcctcgag actggatgaa
     1801 ttacaggatg gtaattgatg gccagagtgt ctattagtga agcggcacgg cttgttaagg
     1861 ttagccgtcc cacaatttac aagatgatta acagtgggaa gttaagttac acctctgttg
     1921 taaagcacgg gaaggctatt aaagttattg atacttcaga gcttattaga gtgtttggtt
     1981 ctcttgatgg tgtcatcgat actgtaaaat atgatgtaaa aagtgacgca gaatctacaa
     2041 gtattaacag cgttggttta catgatttac aacatagaat cgctctactt gaagctgaaa
     2101 acgatggtct taaaggggct gttaaggctc gagatgagca tattgattcc cttcgtcagg
     2161 ctatgcagct tctggagcac aagcgcgagc cttcttcacc accccattcc ccgtggtgga
     2221 aattctggaa aaaatcctga tcctgccatg cttgagtttt cctctccggc catcccaggg
     2281 agttcctgaa ccttcccaaa aatcgccaga tctctgaacg ctcctcacga ctaaaaatac
     2341 ccacacacta cgcaaaatta aaattttgca gattccttat ttaaaacaat tggttaatga
     2401 aaaattaatg tgcagtgatg tgttacaaag tgcagatcca agtcgtggac gactctatgg
     2461 cgaatcgttt acgaattgcc cacgattcat atgtgaatca cgctttatta tcatgttgtt
     2521 atgatgaatc gactcttgat tcaattcatc aatttgggcg ttttttgttc atggtgactc
     2581 ttgattcaat tcacgattcg aatcgccggt gaatcgtgtg gtgagtcgtc cggcgacttg
     2641 ccataacgtg attgccggcc gtcgacgaac ccgattcctg aacgtgctga aaaccggttt
     2701 tccggctgcg ccggcaacgc ctcacggcca cgcaataaat tgcgtccccg ttcaccgttt
     2761 tcggatcaga agaacgccgg aaacaggact taccctggct gaatggcgtg aatttccgcg
     2821 ctatgcactt gcgcgcatac tcatgcatgc cgtaaaaaca gagcctgcgc gtttctggcg
     2881 ggttttcggg tggtttgttg cctgttttac cggtttcccg tcagaaacgt cctgaggccg
     2941 ttttagcggt gcgtacaatt aagggattat ggtaaatggt gacttggtta atcataaaca
     3001 tcctggggga tggcatacac cagttggata tccggtgcat cgttgagcca tcgacctgat
     3061 tttttcatta agtagccacc aatttcttcg gtagtcagcc gggcatgaaa caatgcttca
     3121 tcattgaacc agcagtcagg gtgatagatg agatccagtg ggtttgcctt gaaattaatc
     3181 tccagttact ggagtgcata gctgtggtca gatgcactac cttctgcttt gcgtatggcg
     3241 gatgacctcg attttgacga tattattctg gtgtttcctc cggaatccgg cctaaaaccg
     3301 ctgtatgtga tgtaccgtag ccctcgtaac atgcccggta cagtgagcgg caaagggcag
     3361 aacgttggca ataactggat ggggggggcc agtaccgggg atggtgctcc tgttccttcc
     3421 cagattgcgg ataaattacg cgggaagact ttcggtagtt ttgattcttt ccgtcgggcg
     3481 ttctggaaag cggttgctga cgattctgca ctgagcaagc aatttagtga agctgatatt
     3541 aatcagatga aagctggaag ggcacctact gcggactttt tagaatcagt agggaaacgt
     3601 gttaaaattg aattacacca cgaaaaagaa atatctcaag gaggggctgt tatggatgtc
     3661 gataacatta aggcgctaac acctaaaaat catattgaga cacataaagg aaaataaaat
     3721 ggctggtaat aaaatttcag attttacgga agaagaattt actgcttttg ttagtaaaat
     3781 aatcgctgct gattttccaa ctgacaaaga gaacgatgat gctatttatt cgttcgccca
     3841 actaacagag catcctgctg gttggaacct catcttcaag cctaaggtag gcgaagattc
     3901 ttcggcgaaa ggaattgtta aaaccgtaaa ggaatggcga gctgctaacg gtaagccagg
     3961 ttttaaaaaa gcgtgattga atttgcgggt tcaagttatg agcccgctca tttgtaactc
     4021 tggatttgaa gaataattac tagatatcca tttgcctaga ataataaaaa taaaaggtga
     4081 tatatgaaaa aattactttt cctaggggtt gttcatgtac agaaccttaa gcggcaagcg
     4141 aattaataat accgtctaac gtgttgtttt gcttggattt tctctgccta ctctttttgc
     4201 cgtttttgga taaatacacc attgcccact ccaacttctc tgttatttcc tgccacgcac
     4261 catggatgat gctcgccaat atcggcatta accaaagcgc gctgtttttg gcaatcttca
     4321 gcatcgataa ctccgcgctg ccaatcagcg tcagcgccag ccgacgtttg accagcaagg
     4381 ccagcaaact agcccagatc aagccttccg ctatcgcttt ttgtcgtgtc gcgaacgcct
     4441 tcaaccggtt atgtgatttc agctctttga acaacagttc gatctgccaa cggcaacgat
     4501 acaatgccat caccttctcg gctggccatc gctcacgcgg aagattggtc atccatatac
     4561 agaaccgttt ttcctctgca aaccaacgtc ttatgagtcg atatttatac tttcccgaac
     4621 tcacatccag atccagaacc tcggctctgc agcttctccg atcgatatcc ttgagctttt
     4681 ttccgagtaa cttgggcatt tccctgccgt cctgtcggta agctgcctgt actttgggat
     4741 tgagattttg actccctctg accagatagc ttcctttggc tttggtcaca tcagcaaagt
     4801 actcgcggct gatgtaaccg gcatccgcca ataacagttt gttatttaac gaatttgcgg
     4861 ccggaagatg cttgcgctcc gattcggtat ccgccgtgat actcatcgat atcggcgact
     4921 ggtccagcaa ggacatggtc atatgacact ctaccgcagc ggggctgtgc ttattgaatc
     4981 ggcccgggaa atgttctgcc aattgcggat gtaacgcgaa tgacgacccg tcctgcagca
     5041 gtacctgctc aaagcctgcc agtccgtggg cgtccggtag acactccttg aggcgtaagg
     5101 cgatggctcg ttcaaccagt gcgcgcatga acaagggaaa accctgcttg cgtaactggt
     5161 tgtgaaaggg tttatacgct atttggtcag tatcatggag ctgcaacgca ttgaaatggc
     5221 gatgcaaacc agcaatacca tccaccttgt cgcaaccaag ccctgagagc agagccggga
     5281 gtagtctcgc aggctggata tcccggagtc gctgacaaaa accggtaaat ttaccgatcc
     5341 ggttgagttc agaagagcta agaagttgct tgaggtactg ggttgcattg ataatagtca
     5401 tggcttcggg cgtgtcggtt tttttttgcg attaaatcag atcacaaacg aagacttttt
     5461 tctatctatt tcaattggtt gtagctaaag atcctgtaca tgacaggtca ctgcagcggc
     5521 caaactgttg ccacatctca gttcaggagg aaacgcaatg actcaggaaa ggatccagac
     5581 ccgctaccca ctcggcggcc ccttcgccac ccaggctgag ctggagcagg ccatcaccca
     5641 ggccataacg acccacagac aagatttctt ccctctgctg gaacggctgg tggacgtggg
     5701 ggacacccgg gtcaacctca gtcaggagaa cccgctgcgg gtgctcagca tcgaactcgc
     5761 tgacggccgt gaaggagtgg cccatatcaa ctacgagtcc aacttcgccg agagctgcct
     5821 gattatcaac gaatacgacc agcaccagac cagcctggcc tttcgcctcg acggggatgc
     5881 acttatcttc gacgtcgaac tgccgatcgc gtggaatttc aataactgaa gattcagcgg
     5941 tgtaatgccg cctggttgct acacccggtc agccagctcc cctcacccta accctctccc
     6001 aaagggagag gggatcagcc cgtgccaacc tccagccgtg gtagtcactc catactctgc
     6061 tgttatttca ggcgcctgta atcttcccca tatggctgtc atctgaatag caatatccag
     6121 catatagcca ccataaccct cactcatgta caggatcttt agcggcaacc aattgaaata
     6181 gatataaaaa aggcttcgtt tgtgatctga ttttatcgcc aaacaaaatc aacacgcgcg
     6241 aagcctatga ctattatcaa cgctaattac tatctggagc aactcttggc tcccgccgca
     6301 ctcgagcgca ttgctcggct ctgcaagttt tgtctgcgtc agcgtgccat taccccagcc
     6361 atgctcgtac ctgcactgct gcgggccatg ggaggcgacc aggtcaatga cattgccggt
     6421 ttacaccgcc atttcaatgc cttgcaactg gtaaacaccc atcaggtttc ttacaaaccg
     6481 tttcacaatc agctgcgcaa agagagcttt gccctgttta tgaaagccct ggtcgagcgg
     6541 gccatcgcct tgcgtatcga ccatcaactc aaagcagcct ccctcggcgc gttcaagcag
     6601 gtgttactgc acgatggcac ctcctttgcg gtgcatcggc gccttgctgc tgattttccc
     6661 ggccggttca agaccatcag tccggcagct atcgagtgcc atatgaccat gtcactgtcg
     6721 gagcagagcc cgttgtgcat gagtgtgtct gccgataccg c
//
