LOCUS       NZ_CP026214             6974 bp    DNA     circular CON 13-AUG-2023
DEFINITION  Citrobacter sp. CFNIH10 plasmid pCIT-dfda, complete sequence.
ACCESSION   NZ_CP026214
VERSION     NZ_CP026214.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06040393
            Assembly: GCF_002934585.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. CFNIH10
  ORGANISM  Citrobacter sp. CFNIH10
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   Weingarten,R.A., Johnson,R.C., Conlan,S., Ramsburg,A.M.,
            Dekker,J.P., Lau,A.F., Khil,P., Odom,R.T., Deming,C., Park,M.,
            Thomas,P.J., Henderson,D.K., Palmore,T.N., Segre,J.A. and Frank,K.M.
  CONSRTM   NISC Comparative Sequencing Program
  TITLE     Genomic Analysis of Hospital Plumbing Reveals Diverse Reservoir of
            Bacterial Plasmids Conferring Carbapenem Resistance
  JOURNAL   MBio 9 (1) (2018) In press
REFERENCE   2  (bases 1 to 6974)
  AUTHORS   Segre,J.A. and Mullikin,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2018) NISC, NIH, 5625 Fishers Lane, Rockville, MD
            20852, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 253x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/13/2023 03:58:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,756
            CDSs (total)                      :: 5,634
            Genes (coding)                    :: 5,439
            CDSs (with protein)               :: 5,439
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 41 of 195
            Pseudo Genes (incomplete)         :: 164 of 195
            Pseudo Genes (internal stop)      :: 22 of 195
            Pseudo Genes (multiple problems)  :: 28 of 195
            Pseudo Genes (short protein)      :: 1 of 195
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP026214.1.
            Bacteria available from Karen Frank, Department of Laboratory
            Medicine, National Institutes of Health Clinical Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6974
                     /organism="Citrobacter sp. CFNIH10"
                     /mol_type="genomic DNA"
                     /strain="CFNIH10"
                     /db_xref="taxon:1920110"
                     /plasmid="pCIT-dfda"
                     /geo_loc_name="USA"
                     /lat_lon="38.984652 N 77.094709 W"
                     /collection_date="2016"
     gene            join(6109..6974,1..409)
                     /locus_tag="C2U53_RS03130"
                     /old_locus_tag="C2U53_03125"
     CDS             join(6109..6974,1..409)
                     /locus_tag="C2U53_RS03130"
                     /old_locus_tag="C2U53_03125"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISApu2 family transposase"
                     /protein_id="WP_011191341.1"
                     /translation="MTIINATQYLKQLLSSSELNRIGKFTGFCQRLRDIQPARLLPALL
                     SGLGCDKVDGIAGLHRHFNALQLHDTDQIAYKPFHNQLRKQGFPLFMRALVERAIALRL
                     KECLPDAHGLAGFEQVLLQDGSSFALHPQLAEHFPGRFNKHSPAAVECHMTMSLLDQSP
                     ISMSITADTESERKHLPAANSLNNKLLLADAGYISREYFADVTKAKGSYLVRGSQNLNP
                     KVQAAYRQDGREMPKLLGKKLKDIDRRSCRAEVLDLDVSSGKYKYRLIRRWFAEEKRFC
                     IWMTNLPRERWPAEKVMALYRCRWQIELLFKELKSHNRLKAFATRQKAIAEGLIWASLL
                     ALLVKRRLALTLIGSAELSMLKIAKNSALWLMPILASIIHGAWQEITEKLEWAMVYLSK
                     NGKKSRQRKSKQNNTLDGIINSLAA"
                     /name="IS4-like element ISApu2 family transposase"
     gene            <423..614
                     /locus_tag="C2U53_RS03135"
                     /old_locus_tag="C2U53_03130"
                     /pseudo=""
     CDS             <423..614
                     /locus_tag="C2U53_RS03135"
                     /old_locus_tag="C2U53_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="klebicin B"
                     /name="klebicin B"
     gene            616..873
                     /locus_tag="C2U53_RS03140"
                     /old_locus_tag="C2U53_03135"
     CDS             616..873
                     /locus_tag="C2U53_RS03140"
                     /old_locus_tag="C2U53_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_025714833.1"
                     /translation="MAGNKISDFTEEEFTAFVSKIIAADFPTDKENDDAIYSFAQLTEH
                     PAGWNLIFKPKVGEDSSAKGIVKTVKEWRAANGKPGFKKA"
                     /name="bacteriocin immunity protein"
     gene            981..1136
                     /locus_tag="C2U53_RS03145"
                     /old_locus_tag="C2U53_03140"
     CDS             981..1136
                     /locus_tag="C2U53_RS03145"
                     /old_locus_tag="C2U53_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323621.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071561715.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     gene            1860..2051
                     /locus_tag="C2U53_RS03160"
                     /old_locus_tag="C2U53_03155"
     CDS             1860..2051
                     /locus_tag="C2U53_RS03160"
                     /old_locus_tag="C2U53_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2051..2473
                     /locus_tag="C2U53_RS03165"
                     /old_locus_tag="C2U53_03160"
     CDS             2051..2473
                     /locus_tag="C2U53_RS03165"
                     /old_locus_tag="C2U53_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714834.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3221..>3562)
                     /locus_tag="C2U53_RS03175"
                     /old_locus_tag="C2U53_03170"
                     /pseudo=""
     CDS             complement(3221..>3562)
                     /locus_tag="C2U53_RS03175"
                     /old_locus_tag="C2U53_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            <3561..>3980
                     /locus_tag="C2U53_RS03180"
                     /old_locus_tag="C2U53_03175"
                     /pseudo=""
     CDS             <3561..>3980
                     /locus_tag="C2U53_RS03180"
                     /old_locus_tag="C2U53_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /name="S-type pyocin domain-containing protein"
     gene            complement(3995..5263)
                     /locus_tag="C2U53_RS03185"
                     /old_locus_tag="C2U53_03180"
     CDS             complement(3995..5263)
                     /locus_tag="C2U53_RS03185"
                     /old_locus_tag="C2U53_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011191342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISApu1 family transposase"
                     /protein_id="WP_103215790.1"
                     /translation="MTIINANYYLEQLLAPAALERIARLCKFCLRQRAITPAMLVPALL
                     RAMGGDQVNDIAGLHRHFNALQLVNTHQVSYKPFHNQLRKESFALFMKALVERAIALRI
                     DHQLKAASLGAFKQVLLHDGTSFAVHRRLAADFPGRFKTISPAAIECHMTMSLSEQSPL
                     CMSVSADTAGERQFLPEAHTLTNCLLLADAGYIDRAWFEQVNDAGGFYLVRGTKSLNPK
                     IIQAWRGDGREVPKLAGLSLREAGRRRCRAEVLDMVVKSGQVEYRLIRRWFAEEKRFCL
                     WMTNLPRAAWSAEQVMSLYRCRWQVELLFKEWKSHNRLKGFVTGEKAIAEGLVWTSLLS
                     LVMKRRVAQSVMSGALSMLKASKNSATWWLPLLEAVAHMALTEIRERLEWAADYLAKNA
                     CRTKQRKSIQNRTLEGVLNGLAA"
                     /name="IS4-like element ISApu1 family transposase"
     gene            complement(5581..5952)
                     /locus_tag="C2U53_RS03190"
                     /old_locus_tag="C2U53_03185"
     CDS             complement(5581..5952)
                     /locus_tag="C2U53_RS03190"
                     /old_locus_tag="C2U53_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019838990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004099020.1"
                     /translation="MTQERIQTRYPLGGPFATQAELEQAITQAITTHRQDFFPLLERLV
                     DVGDTRVNLSQENPLRVLSIELADGREGVAHINYESNFAESCLIINEYDQHQTSLAFRL
                     DGDALIFDVELPIAWNFNN"
                     /name="hypothetical protein"
     Typing          complement(1343..1812)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.834"
                     /coverage="92.0"
                     /evalue="2.73e-170"
ORIGIN
        1 tgagcgatgg ccagccgaga aggtgatggc attgtatcgt tgccgttggc agatcgaact
       61 gttgttcaaa gagctgaaat cacataaccg gttgaaggcg ttcgcgacac gacaaaaagc
      121 gatagcggaa ggcttgatct gggctagttt gctggccttg ctggtcaaac gtcggctggc
      181 gctgacgctg attggcagcg cggagttatc gatgctgaag attgccaaaa acagcgcgct
      241 ttggttaatg ccgatattgg cgagcatcat ccatggtgcg tggcaggaaa taacagagaa
      301 gttggagtgg gcaatggtgt atttatccaa aaacggcaaa aagagtaggc agagaaaatc
      361 caagcaaaac aacacgttag acggtattat taattcgctt gccgcttaag gttctgtaca
      421 tgagtgaagc tgatattaat cagatgaaag ctggaagggc acctactgcg gactttttag
      481 aatcagtagg gaaacgtgtt aaaattgaat tacaccacga aaaagaaata tctcaaggag
      541 gggctgttat ggatgtcgat aacattaagg cgctaacacc taaaaatcat attgagacac
      601 ataaaggaaa ataaaatggc tggtaataaa atttcagatt ttacggaaga agaatttact
      661 gcttttgtta gtaaaataat cgctgctgat tttccaactg acaaagagaa cgatgatgct
      721 atttattcgt tcgcccaact aacagagcat cctgctggtt ggaacctcat cttcaagcct
      781 aaggtaggcg aagattcttc ggcgaaagga attgttaaaa ccgtaaagga atggcgagct
      841 gctaacggta agccaggttt taaaaaagcg tgattgaatt tgcgggttca agttatgagc
      901 ccgctcattt gtaactctgg atttgaagaa taattactag atatccattt gcctagaata
      961 ataaaaataa aaggtgatat atgaaaaaat tacttttcct aggggttgtt accgtttctg
     1021 tggttctggc agcatgtcag gtcaataatg ttcgagacac cggaggtggg gctgtttcac
     1081 cttcatcgac ggtgaccgga gtcacgcttg atagcggtac gaacggcaat ccgtaaagga
     1141 tcttcttgag atcccatttg gatcgtcgta atctcttgct ctgtaaacga aaaaaccgcc
     1201 ttgcagggcg gtttttccga aggttcaaac acttgcaggt gattgaacca aggtaactgg
     1261 cttagaggag cgcagtcacc aaaacttgtt ctttcagttt agccttaaca ggcgcatgac
     1321 ttcaagacca actcctctaa atccgttacc agtggctgct gccagtggcg ctttgtcgtg
     1381 tctttccggg ttggactcaa gatgatagtt accggataag acgcagcagt cggactgaac
     1441 ggggggttcg tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag
     1501 gcgtggaatg agacaaacgc ggccataaca gcggaatgac accggtaaac cgaatggcag
     1561 gaacaggaga gcgcacgagg gagccatcag ggggaaacgc ctggtatctt tatagtcctg
     1621 tcgggtttcg ccaccactga tttgagcgtc aaattctgtg atgcttgtca ggggggcgga
     1681 gcctatggaa aaacggccgc tgtgctgcct tctccctctc cgagctgtct gctctatgtc
     1741 gttatgtgcg cgttgcgacc gctcgccgca gccgaacggc aggagcgtag cgactgagtg
     1801 agcgaggaag cggaaaagag atctgtctga cattgagcac tgacgctctg aggcccttta
     1861 tgacgaaaca acagaaaaca gcgctgaaca tggctaaatt catccagaat cagtctctgc
     1921 tcctgctgga gaagcttaac gagcttgatc tcgatgttga ggccgacctt tgtgagaaac
     1981 tccacgatga tgcggagcat cttttccgta cactgtcctc gagactggat gaattacagg
     2041 atggcaattg atggccagag tgtctattag tgaagcggca cggcttgtta aggttagccg
     2101 tcccacaatt tacaagatga ttaacagtgg gaagttaagt tacacctctg ttgtaaagca
     2161 cgggaaggct attaaagtta ttgatacttc agagcttatt agagtttttg gttctcttga
     2221 tggtgtcatc gatactgtaa aatatgatgt aaaaagtgac gcagaatcta caagtattaa
     2281 cagcgttggt ttacatgatt tacaacatag aatcgctcta cttgaagctg aaaacgatgg
     2341 tcttaaaggg gctgttaagg ctcgagatga gcatattgat tcccttcgtc aggctatgca
     2401 gcttctggag cacaagcgcg agccttcttc accaccccat tccccgtggt ggaaattctg
     2461 gaaaaaatcc tgatcctgcc atgcttgagt tttcctctcc ggccatccca gggagttcct
     2521 gaaccttccc aaaaatcgcc agatctctga acgctcctca cgactaaaaa tacccacaca
     2581 ctacgcaaaa ttaaaatttt gcagattcct tatttaaaac aattggttaa tgaaaaatta
     2641 atgtgcagtg atgtgttaca aagtgcagat ccaagtcgtg gacgactcta tggcgaatcg
     2701 tttacgaatt gcccacgatt catatgtgaa tcacgcttta ttatcatgtt gttatgatga
     2761 atcgactctt gattcaattc atcaatttgg gcgttttttg ttcatggtga ctcttgattc
     2821 aattcacgat tcgaatcgcc ggtgaatcgt gtggtgagtc gtccggcgac ttgccataac
     2881 gtgattgccg gccgtcgacg aacccgattc ctgaacgtgc tgaaaaccgg ttttccggct
     2941 gcgccggcaa cgcctcacgg ccacgcaata aattgcgtcc ccgttcaccg ttttcggatc
     3001 agaagaacgc cggaaacagg acttaccctg gctgaatggc gtgaatttcc gcgctatgca
     3061 cttgcgcgca tactcatgca tgccgtaaaa acagagcctg cgcgtttctg gcgggttttc
     3121 gggtggtttg ttgcctgttt taccggtttc ccgtcagaaa cgtcctgagg ccgttttagc
     3181 ggtgcgtaca attaagggat tatggtaaat ggtgacttgg ttaatcataa acatcctggg
     3241 ggatggcata caccagttgg atatccggtg catcgttgag ccatcgacct gattttttca
     3301 ttaagtagcc accaatttct tcggtagtca gccgggcatg aaacaatgct tcatcattga
     3361 accagcagtc agggtgatag atgagatcca gtgggtttgc cttgaaatta atctccagta
     3421 actggagtgc atagctgtgg tcagatgcac taccttctgc tttgcgtatg aagttgataa
     3481 cgtcgttcag ttctttccat gtaaagtcat cgaggtattt ttcctgatta agcgccatcc
     3541 tggtgaaatc tagtgcactt gacgcacctg gtgcaaacgg accactgacg ctaagcgggc
     3601 cggtaccact accggagcgt attctgcata ccggtgatca gatttcggcg ccacaggcta
     3661 cagataaaac cgtgacgccg gtagcggatg acctcgattt tgacgatatt attctggtgt
     3721 ttcctccgga atccggccta aaaccgctgt atgtgatgta ccgtagccct cgtaacatgc
     3781 ccggtacagt gagcggcaaa gggcagaacg ttggcaataa ctggatgggg ggggccagta
     3841 ccggggatgg tgctcctgtt ccttcccaga ttgcggataa attacgcggg aagactttcg
     3901 gtagttttga ttctttccgt cgggcgttct ggaaagcggt tgctgacgat tctgcactga
     3961 gcaagcaatt tagtgaagct gcatgtacag gaccttaagc ggcgagtcca tttaaaacgc
     4021 cttccaaggt cctgttctgt atcgactttc tctgcttggt tcggcaggcg ttcttggcca
     4081 aataatcggc agcccattcc aatctttccc ttatttctgt cagcgccata tgggccactg
     4141 cttccaatag cggtagccac cacgtcgcac tgttcttcga cgctttcagc atcgatagcg
     4201 ccccactcat cacactctgc gcgacccgtc tcttcatcac cagcgacagc aggctcgtcc
     4261 acaccaaccc ctcggcaatc gctttctcgc ccgtcacaaa ccccttgagc cggttgtgcg
     4321 atttccactc tttaaataac aactccacct gccaacggca gcgatacagg ctcatcacct
     4381 gctctgctga ccatgctgct cgcggtaggt tcgtcatcca cagacagaac cgcttttcct
     4441 cagcaaacca gcgccggatc aaccggtact cgacctggcc ggacttcacc accatatcca
     4501 gtacctcggc ccgacagcgt cgccgtccgg cctctctcag agatagtccc gccagcttgg
     4561 gtacttcccg tccatcaccg cgccaggctt ggattatctt ggggttcagg cttttcgtgc
     4621 cgcgcaccag ataaaatccg ccagcgtcgt tcacctgctc aaaccaagcc ctgtcaatgt
     4681 aacctgcgtc agccagcagc aggcagttgg tcagcgtgtg ggcttcaggc agaaattgcc
     4741 gttcccctgc ggtatcggca gacacactca tgcacaacgg gctctgctcc gacagtgaca
     4801 tggtcatatg gcactcgata gctgccggac tgatggtctt gaaccggccg ggaaaatcag
     4861 cagcaaggcg ccgatgcacc gcaaaggagg tgccatcgtg cagtaacacc tgcttgaacg
     4921 cgccgaggga ggctgctttg agttgatggt cgatacgcaa ggcgatggcc cgctcgacca
     4981 gggctttcat aaacagggca aagctctctt tgcgcagctg attgtgaaac ggtttgtaag
     5041 aaacctgatg ggtgtttacc agttgcaagg cattgaaatg gcggtgtaaa ccggcaatgt
     5101 cattgacctg gtcgcctccc atggcccgca gcagtgcagg tacgagcatg gctggggtaa
     5161 tggcacgctg acgcagacaa aacttgcaga gccgagcaat gcgctcgagt gcggcgggag
     5221 ccaagagttg ctccagatag taattagcgt tgataatagt cataggcttc gcgcgtgttg
     5281 attttgtttg gcgataaaat cagatcacaa acgaagcctt ttttatatct atttcaattg
     5341 gttgccgcta aagatcctgt acatgagtga gggttatggt ggctatatgc tggatattgc
     5401 tattcagatg acagccatat ggggaagatt acaggcgcct gaaataacag cagagtatgg
     5461 agtgactacc acggctggag gttggcacgg gctgatcccc tctccctttg ggagagggtt
     5521 agggtgaggg gagctggctg accgggtgta gcaaccaggc ggcattacac cgctgaatct
     5581 tcagttattg aaattccacg cgatcggcag ttcgacgtcg aagataagtg catccccgtc
     5641 gaggcgaaag gccaggctgg tctggtgctg gtcgtattcg ttgataatca ggcagctctc
     5701 ggcgaagttg gactcgtagt tgatatgggc cactccttca cggccgtcag cgagttcgat
     5761 gctgagcacc cgcagcgggt tctcctgact gaggttgacc cgggtgtccc ccacgtccac
     5821 cagccgttcc agcagaggga agaaatcttg tctgtgggtc gttatggcct gggtgatggc
     5881 ctgctccagc tcagcctggg tggcgaaggg gccgccgagt gggtagcggg tctggatcct
     5941 ttcctgagtc attgcgtttc ctcctgaact gagatgtggc aacagtttgg ccgctgcagt
     6001 gacctgtcat gtacaggatc tttagctaca accaattgaa atagatataa aaaagtcttc
     6061 gtttgtgatc tgatttaatc gcaaaaaaaa accgacacgc ccgaagccat gactattatc
     6121 aatgcaaccc agtacctcaa gcaacttctt agctcttctg aactcaaccg gatcggtaaa
     6181 tttaccggtt tttgtcagcg actccgggat atccagcctg cgagactact cccggctctg
     6241 ctctcagggc ttggttgcga caaggtggat ggtattgctg gtttgcatcg ccatttcaat
     6301 gcgttgcagc tccatgatac tgaccaaata gcgtataaac cctttcacaa ccagttacgc
     6361 aagcagggtt ttcccttgtt catgcgcgca ctggttgaac gagccatcgc cttacgcctc
     6421 aaggagtgtc taccggacgc ccacggactg gcaggctttg agcaggtact gctgcaggac
     6481 gggtcgtcat tcgcgttaca tccgcaattg gcagaacatt tcccgggccg attcaataag
     6541 cacagccccg ctgcggtaga gtgtcatatg accatgtcct tgctggacca gtcgccgata
     6601 tcgatgagta tcacggcgga taccgaatcg gagcgcaagc atcttccggc cgcaaattcg
     6661 ttaaataaca aactgttatt ggcggatgcc ggttacatca gccgcgagta ctttgctgat
     6721 gtgaccaaag ccaaaggaag ctatctggtc agagggagtc aaaatctcaa tcccaaagta
     6781 caggcagctt accgacagga cggcagggaa atgcccaagt tactcggaaa aaagctcaag
     6841 gatatcgatc ggagaagctg cagagccgag gttctggatc tggatgtgag ttcgggaaag
     6901 tataaatatc gactcataag acgttggttt gcagaggaaa aacggttctg tatatggatg
     6961 accaatcttc cgcg
//
