LOCUS       NZ_CP026183             6974 bp    DNA     circular CON 25-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KPNIH49 plasmid pKPN-dfda, complete
            sequence.
ACCESSION   NZ_CP026183
VERSION     NZ_CP026183.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06040388
            Assembly: GCF_002903025.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   Weingarten,R.A., Johnson,R.C., Conlan,S., Ramsburg,A.M.,
            Dekker,J.P., Lau,A.F., Khil,P., Odom,R.T., Deming,C., Park,M.,
            Thomas,P.J., Henderson,D.K., Palmore,T.N., Segre,J.A. and Frank,K.M.
  CONSRTM   NISC Comparative Sequencing Program
  TITLE     Genomic Analysis of Hospital Plumbing Reveals Diverse Reservoir of
            Bacterial Plasmids Conferring Carbapenem Resistance
  JOURNAL   mBio 9 (1) (2018) In press
REFERENCE   2  (bases 1 to 6974)
  AUTHORS   Segre,J.A. and Mullikin,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2018) NISC, NIH, 5625 Fishers Lane, Rockville, MD
            20852, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/25/2023 04:57:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,475
            CDSs (total)                      :: 6,351
            Genes (coding)                    :: 6,072
            CDSs (with protein)               :: 6,072
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 280
            CDSs (without protein)            :: 279
            Pseudo Genes (ambiguous residues) :: 0 of 280
            Pseudo Genes (frameshifted)       :: 103 of 280
            Pseudo Genes (incomplete)         :: 199 of 280
            Pseudo Genes (internal stop)      :: 40 of 280
            Pseudo Genes (multiple problems)  :: 55 of 280
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP026183.1.
            Bacteria available from Karen Frank, Department of Laboratory
            Medicine, National Institutes of Health Clinical Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6974
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPNIH49"
                     /db_xref="taxon:573"
                     /plasmid="pKPN-dfda"
                     /geo_loc_name="USA"
                     /lat_lon="38.984652 N 77.094709 W"
                     /collection_date="2016"
     gene            242..403
                     /locus_tag="C2U49_RS35310"
                     /old_locus_tag="C2U49_30780"
     CDS             242..403
                     /locus_tag="C2U49_RS35310"
                     /old_locus_tag="C2U49_30780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049165871.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPERHDKAPLAAATGNGFRGVGLE
                     VMRLLRLN"
                     /name="hypothetical protein"
     gene            complement(561..716)
                     /locus_tag="C2U49_RS30780"
                     /old_locus_tag="C2U49_30785"
     CDS             complement(561..716)
                     /locus_tag="C2U49_RS30780"
                     /old_locus_tag="C2U49_30785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323621.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071561715.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     gene            complement(824..1081)
                     /locus_tag="C2U49_RS30785"
                     /old_locus_tag="C2U49_30790"
     CDS             complement(824..1081)
                     /locus_tag="C2U49_RS30785"
                     /old_locus_tag="C2U49_30790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_025714833.1"
                     /translation="MAGNKISDFTEEEFTAFVSKIIAADFPTDKENDDAIYSFAQLTEH
                     PAGWNLIFKPKVGEDSSAKGIVKTVKEWRAANGKPGFKKA"
                     /name="bacteriocin immunity protein"
     gene            complement(1083..>1274)
                     /locus_tag="C2U49_RS30790"
                     /old_locus_tag="C2U49_30795"
                     /pseudo=""
     CDS             complement(1083..>1274)
                     /locus_tag="C2U49_RS30790"
                     /old_locus_tag="C2U49_30795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="klebicin B"
                     /name="klebicin B"
     gene            complement(1288..2562)
                     /locus_tag="C2U49_RS30795"
                     /old_locus_tag="C2U49_30800"
     CDS             complement(1288..2562)
                     /locus_tag="C2U49_RS30795"
                     /old_locus_tag="C2U49_30800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011222061.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISApu2 family transposase"
                     /protein_id="WP_011191341.1"
                     /translation="MTIINATQYLKQLLSSSELNRIGKFTGFCQRLRDIQPARLLPALL
                     SGLGCDKVDGIAGLHRHFNALQLHDTDQIAYKPFHNQLRKQGFPLFMRALVERAIALRL
                     KECLPDAHGLAGFEQVLLQDGSSFALHPQLAEHFPGRFNKHSPAAVECHMTMSLLDQSP
                     ISMSITADTESERKHLPAANSLNNKLLLADAGYISREYFADVTKAKGSYLVRGSQNLNP
                     KVQAAYRQDGREMPKLLGKKLKDIDRRSCRAEVLDLDVSSGKYKYRLIRRWFAEEKRFC
                     IWMTNLPRERWPAEKVMALYRCRWQIELLFKELKSHNRLKAFATRQKAIAEGLIWASLL
                     ALLVKRRLALTLIGSAELSMLKIAKNSALWLMPILASIIHGAWQEITEKLEWAMVYLSK
                     NGKKSRQRKSKQNNTLDGIINSLAA"
                     /name="IS4-like element ISApu2 family transposase"
     gene            2719..3090
                     /locus_tag="C2U49_RS30800"
                     /old_locus_tag="C2U49_30805"
     CDS             2719..3090
                     /locus_tag="C2U49_RS30800"
                     /old_locus_tag="C2U49_30805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019838990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004099020.1"
                     /translation="MTQERIQTRYPLGGPFATQAELEQAITQAITTHRQDFFPLLERLV
                     DVGDTRVNLSQENPLRVLSIELADGREGVAHINYESNFAESCLIINEYDQHQTSLAFRL
                     DGDALIFDVELPIAWNFNN"
                     /name="hypothetical protein"
     gene            3408..4676
                     /locus_tag="C2U49_RS30805"
                     /old_locus_tag="C2U49_30810"
     CDS             3408..4676
                     /locus_tag="C2U49_RS30805"
                     /old_locus_tag="C2U49_30810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011191342.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISApu1 family transposase"
                     /protein_id="WP_103215790.1"
                     /translation="MTIINANYYLEQLLAPAALERIARLCKFCLRQRAITPAMLVPALL
                     RAMGGDQVNDIAGLHRHFNALQLVNTHQVSYKPFHNQLRKESFALFMKALVERAIALRI
                     DHQLKAASLGAFKQVLLHDGTSFAVHRRLAADFPGRFKTISPAAIECHMTMSLSEQSPL
                     CMSVSADTAGERQFLPEAHTLTNCLLLADAGYIDRAWFEQVNDAGGFYLVRGTKSLNPK
                     IIQAWRGDGREVPKLAGLSLREAGRRRCRAEVLDMVVKSGQVEYRLIRRWFAEEKRFCL
                     WMTNLPRAAWSAEQVMSLYRCRWQVELLFKEWKSHNRLKGFVTGEKAIAEGLVWTSLLS
                     LVMKRRVAQSVMSGALSMLKASKNSATWWLPLLEAVAHMALTEIRERLEWAADYLAKNA
                     CRTKQRKSIQNRTLEGVLNGLAA"
                     /name="IS4-like element ISApu1 family transposase"
     gene            complement(<4691..>5110)
                     /locus_tag="C2U49_RS30810"
                     /old_locus_tag="C2U49_30815"
                     /pseudo=""
     CDS             complement(<4691..>5110)
                     /locus_tag="C2U49_RS30810"
                     /old_locus_tag="C2U49_30815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /name="S-type pyocin domain-containing protein"
     gene            <5109..5450
                     /locus_tag="C2U49_RS30815"
                     /old_locus_tag="C2U49_30820"
                     /pseudo=""
     CDS             <5109..5450
                     /locus_tag="C2U49_RS30815"
                     /old_locus_tag="C2U49_30820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(6198..6620)
                     /locus_tag="C2U49_RS30825"
                     /old_locus_tag="C2U49_30830"
     CDS             complement(6198..6620)
                     /locus_tag="C2U49_RS30825"
                     /old_locus_tag="C2U49_30830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714834.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(6620..6811)
                     /locus_tag="C2U49_RS30830"
                     /old_locus_tag="C2U49_30835"
     CDS             complement(6620..6811)
                     /locus_tag="C2U49_RS30830"
                     /old_locus_tag="C2U49_30835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2..352
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.875"
                     /coverage="86.0"
                     /evalue="5.97e-166"
ORIGIN
        1 ccgtttttcc ataggctccg cccccctgac aagcatcaca gaatttgacg ctcaaatcag
       61 tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctga tggctccctc
      121 gtgcgctctc ctgttcctgc cattcggttt accggtgtca ttccgctgtt atggccgcgt
      181 ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg
      241 tatgcacgaa ccccccgttc agtccgactg ctgcgtctta tccggtaact atcatcttga
      301 gtccaacccg gaaagacacg acaaagcgcc actggcagca gccactggta acggatttag
      361 aggagttggt cttgaagtca tgcgcctgtt aaggctaaac tgaaagaaca agttttggtg
      421 actgcgctcc tctaagccag ttaccttggt tcaatcacct gcaagtgttt gaaccttcgg
      481 aaaaaccgcc ctgcaaggcg gttttttcgt ttacagagca agagattacg acgatccaaa
      541 tgggatctca agaagatcct ttacggattg ccgttcgtac cgctatcaag cgtgactccg
      601 gtcaccgtcg atgaaggtga aacagcccca cctccggtgt ctcgaacatt attgacctga
      661 catgctgcca gaaccacaga aacggtaaca acccctagga aaagtaattt tttcatatat
      721 caccttttat ttttattatt ctaggcaaat ggatatctag taattattct tcaaatccag
      781 agttacaaat gagcgggctc ataacttgaa cccgcaaatt caatcacgct tttttaaaac
      841 ctggcttacc gttagcagct cgccattcct ttacggtttt aacaattcct ttcgccgaag
      901 aatcttcgcc taccttaggc ttgaagatga ggttccaacc agcaggatgc tctgttagtt
      961 gggcgaacga ataaatagca tcatcgttct ctttgtcagt tggaaaatca gcagcgatta
     1021 ttttactaac aaaagcagta aattcttctt ccgtaaaatc tgaaatttta ttaccagcca
     1081 ttttattttc ctttatgtgt ctcaatatga tttttaggtg ttagcgcctt aatgttatcg
     1141 acatccataa cagcccctcc ttgagatatt tctttttcgt ggtgtaattc aattttaaca
     1201 cgtttcccta ctgattctaa aaagtccgca gtaggtgccc ttccagcttt catctgatta
     1261 atatcagctt cactcatgta cagaacctta agcggcaagc gaattaataa taccgtctaa
     1321 cgtgttgttt tgcttggatt ttctctgcct actctttttg ccgtttttgg ataaatacac
     1381 cattgcccac tccaacttct ctgttatttc ctgccacgca ccatggatga tgctcgccaa
     1441 tatcggcatt aaccaaagcg cgctgttttt ggcaatcttc agcatcgata actccgcgct
     1501 gccaatcagc gtcagcgcca gccgacgttt gaccagcaag gccagcaaac tagcccagat
     1561 caagccttcc gctatcgctt tttgtcgtgt cgcgaacgcc ttcaaccggt tatgtgattt
     1621 cagctctttg aacaacagtt cgatctgcca acggcaacga tacaatgcca tcaccttctc
     1681 ggctggccat cgctcacgcg gaagattggt catccatata cagaaccgtt tttcctctgc
     1741 aaaccaacgt cttatgagtc gatatttata ctttcccgaa ctcacatcca gatccagaac
     1801 ctcggctctg cagcttctcc gatcgatatc cttgagcttt tttccgagta acttgggcat
     1861 ttccctgccg tcctgtcggt aagctgcctg tactttggga ttgagatttt gactccctct
     1921 gaccagatag cttcctttgg ctttggtcac atcagcaaag tactcgcggc tgatgtaacc
     1981 ggcatccgcc aataacagtt tgttatttaa cgaatttgcg gccggaagat gcttgcgctc
     2041 cgattcggta tccgccgtga tactcatcga tatcggcgac tggtccagca aggacatggt
     2101 catatgacac tctaccgcag cggggctgtg cttattgaat cggcccggga aatgttctgc
     2161 caattgcgga tgtaacgcga atgacgaccc gtcctgcagc agtacctgct caaagcctgc
     2221 cagtccgtgg gcgtccggta gacactcctt gaggcgtaag gcgatggctc gttcaaccag
     2281 tgcgcgcatg aacaagggaa aaccctgctt gcgtaactgg ttgtgaaagg gtttatacgc
     2341 tatttggtca gtatcatgga gctgcaacgc attgaaatgg cgatgcaaac cagcaatacc
     2401 atccaccttg tcgcaaccaa gccctgagag cagagccggg agtagtctcg caggctggat
     2461 atcccggagt cgctgacaaa aaccggtaaa tttaccgatc cggttgagtt cagaagagct
     2521 aagaagttgc ttgaggtact gggttgcatt gataatagtc atggcttcgg gcgtgtcggt
     2581 ttttttttgc gattaaatca gatcacaaac gaagactttt ttatatctat ttcaattggt
     2641 tgtagctaaa gatcctgtac atgacaggtc actgcagcgg ccaaactgtt gccacatctc
     2701 agttcaggag gaaacgcaat gactcaggaa aggatccaga cccgctaccc actcggcggc
     2761 cccttcgcca cccaggctga gctggagcag gccatcaccc aggccataac gacccacaga
     2821 caagatttct tccctctgct ggaacggctg gtggacgtgg gggacacccg ggtcaacctc
     2881 agtcaggaga acccgctgcg ggtgctcagc atcgaactcg ctgacggccg tgaaggagtg
     2941 gcccatatca actacgagtc caacttcgcc gagagctgcc tgattatcaa cgaatacgac
     3001 cagcaccaga ccagcctggc ctttcgcctc gacggggatg cacttatctt cgacgtcgaa
     3061 ctgccgatcg cgtggaattt caataactga agattcagcg gtgtaatgcc gcctggttgc
     3121 tacacccggt cagccagctc ccctcaccct aaccctctcc caaagggaga ggggatcagc
     3181 ccgtgccaac ctccagccgt ggtagtcact ccatactctg ctgttatttc aggcgcctgt
     3241 aatcttcccc atatggctgt catctgaata gcaatatcca gcatatagcc accataaccc
     3301 tcactcatgt acaggatctt tagcggcaac caattgaaat agatataaaa aaggcttcgt
     3361 ttgtgatctg attttatcgc caaacaaaat caacacgcgc gaagcctatg actattatca
     3421 acgctaatta ctatctggag caactcttgg ctcccgccgc actcgagcgc attgctcggc
     3481 tctgcaagtt ttgtctgcgt cagcgtgcca ttaccccagc catgctcgta cctgcactgc
     3541 tgcgggccat gggaggcgac caggtcaatg acattgccgg tttacaccgc catttcaatg
     3601 ccttgcaact ggtaaacacc catcaggttt cttacaaacc gtttcacaat cagctgcgca
     3661 aagagagctt tgccctgttt atgaaagccc tggtcgagcg ggccatcgcc ttgcgtatcg
     3721 accatcaact caaagcagcc tccctcggcg cgttcaagca ggtgttactg cacgatggca
     3781 cctcctttgc ggtgcatcgg cgccttgctg ctgattttcc cggccggttc aagaccatca
     3841 gtccggcagc tatcgagtgc catatgacca tgtcactgtc ggagcagagc ccgttgtgca
     3901 tgagtgtgtc tgccgatacc gcaggggaac ggcaatttct gcctgaagcc cacacgctga
     3961 ccaactgcct gctgctggct gacgcaggtt acattgacag ggcttggttt gagcaggtga
     4021 acgacgctgg cggattttat ctggtgcgcg gcacgaaaag cctgaacccc aagataatcc
     4081 aagcctggcg cggtgatgga cgggaagtac ccaagctggc gggactatct ctgagagagg
     4141 ccggacggcg acgctgtcgg gccgaggtac tggatatggt ggtgaagtcc ggccaggtcg
     4201 agtaccggtt gatccggcgc tggtttgctg aggaaaagcg gttctgtctg tggatgacga
     4261 acctaccgcg agcagcatgg tcagcagagc aggtgatgag cctgtatcgc tgccgttggc
     4321 aggtggagtt gttatttaaa gagtggaaat cgcacaaccg gctcaagggg tttgtgacgg
     4381 gcgagaaagc gattgccgag gggttggtgt ggacgagcct gctgtcgctg gtgatgaaga
     4441 gacgggtcgc gcagagtgtg atgagtgggg cgctatcgat gctgaaagcg tcgaagaaca
     4501 gtgcgacgtg gtggctaccg ctattggaag cagtggccca tatggcgctg acagaaataa
     4561 gggaaagatt ggaatgggct gccgattatt tggccaagaa cgcctgccga accaagcaga
     4621 gaaagtcgat acagaacagg accttggaag gcgttttaaa tggactcgcc gcttaaggtc
     4681 ctgtacatgc agcttcacta aattgcttgc tcagtgcaga atcgtcagca accgctttcc
     4741 agaacgcccg acggaaagaa tcaaaactac cgaaagtctt cccgcgtaat ttatccgcaa
     4801 tctgggaagg aacaggagca ccatccccgg tactggcccc ccccatccag ttattgccaa
     4861 cgttctgccc tttgccgctc actgtaccgg gcatgttacg agggctacgg tacatcacat
     4921 acagcggttt taggccggat tccggaggaa acaccagaat aatatcgtca aaatcgaggt
     4981 catccgctac cggcgtcacg gttttatctg tagcctgtgg cgccgaaatc tgatcaccgg
     5041 tatgcagaat acgctccggt agtggtaccg gcccgcttag cgtcagtggt ccgtttgcac
     5101 caggtgcgtc aagtgcacta gatttcacca ggatggcgct taatcaggaa aaatacctcg
     5161 atgactttac atggaaagaa ctgaacgacg ttatcaactt catacgcaaa gcagaaggta
     5221 gtgcatctga ccacagctat gcactccagt tactggagat taatttcaag gcaaacccac
     5281 tggatctcat ctatcaccct gactgctggt tcaatgatga agcattgttt catgcccggc
     5341 tgactaccga agaaattggt ggctacttaa tgaaaaaatc aggtcgatgg ctcaacgatg
     5401 caccggatat ccaactggtg tatgccatcc cccaggatgt ttatgattaa ccaagtcacc
     5461 atttaccata atcccttaat tgtacgcacc gctaaaacgg cctcaggacg tttctgacgg
     5521 gaaaccggta aaacaggcaa caaaccaccc gaaaacccgc cagaaacgcg caggctctgt
     5581 ttttacggca tgcatgagta tgcgcgcaag tgcatagcgc ggaaattcac gccattcagc
     5641 cagggtaagt cctgtttccg gcgttcttct gatccgaaaa cggtgaacgg ggacgcaatt
     5701 tattgcgtgg ccgtgaggcg ttgccggcgc agccggaaaa ccggttttca gcacgttcag
     5761 gaatcgggtt cgtcgacggc cggcaatcac gttatggcaa gtcgccggac gactcaccac
     5821 acgattcacc ggcgattcga atcgtgaatt gaatcaagag tcaccatgaa caaaaaacgc
     5881 ccaaattgat gaattgaatc aagagtcgat tcatcataac aacatgataa taaagcgtga
     5941 ttcacatatg aatcgtgggc aattcgtaaa cgattcgcca tagagtcgtc cacgacttgg
     6001 atctgcactt tgtaacacat cactgcacat taatttttca ttaaccaatt gttttaaata
     6061 aggaatctgc aaaattttaa ttttgcgtag tgtgtgggta tttttagtcg tgaggagcgt
     6121 tcagagatct ggcgattttt gggaaggttc aggaactccc tgggatggcc ggagaggaaa
     6181 actcaagcat ggcaggatca ggattttttc cagaatttcc accacgggga atggggtggt
     6241 gaagaaggct cgcgcttgtg ctccagaagc tgcatagcct gacgaaggga atcaatatgc
     6301 tcatctcgag ccttaacagc ccctttaaga ccatcgtttt cagcttcaag tagagcgatt
     6361 ctatgttgta aatcatgtaa accaacgctg ttaatacttg tagattctgc gtcacttttt
     6421 acatcatatt ttacagtatc gatgacacca tcaagagaac caaaaactct aataagctct
     6481 gaagtatcaa taactttaat agccttcccg tgctttacaa cagaggtgta acttaacttc
     6541 ccactgttaa tcatcttgta aattgtggga cggctaacct taacaagccg tgccgcttca
     6601 ctaatagaca ctctggccat caattgccat cctgtaattc atccagtctc gaggacagtg
     6661 tacggaaaag atgctccgca tcatcgtgga gtttctcaca aaggtcggcc tcaacatcga
     6721 gatcaagctc gttaagcttc tccagcagga gcagagactg attctggatg aatttagcca
     6781 tgttcagcgc tgttttctgt tgtttcgtca taaagggcct cagagcgtca gtgctcaatg
     6841 tcagacagat ctcttttccg cttcctcgct cactcagtcg ctacgctcct gccgttcggc
     6901 tgcggcgagc ggtcgcaacg cgcacataac gacatagagc agacagctcg gagagggaga
     6961 aggcagcaca gcgg
//
