LOCUS       NZ_CP026176            18977 bp    DNA     circular CON 25-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KPNIH50 plasmid pKPN-bb08, complete
            sequence.
ACCESSION   NZ_CP026176
VERSION     NZ_CP026176.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06040389
            Assembly: GCF_002903005.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 18977)
  AUTHORS   Weingarten,R.A., Johnson,R.C., Conlan,S., Ramsburg,A.M.,
            Dekker,J.P., Lau,A.F., Khil,P., Odom,R.T., Deming,C., Park,M.,
            Thomas,P.J., Henderson,D.K., Palmore,T.N., Segre,J.A. and Frank,K.M.
  CONSRTM   NISC Comparative Sequencing Program
  TITLE     Genomic Analysis of Hospital Plumbing Reveals Diverse Reservoir of
            Bacterial Plasmids Conferring Carbapenem Resistance
  JOURNAL   mBio 9 (1) (2018) In press
REFERENCE   2  (bases 1 to 18977)
  AUTHORS   Segre,J.A. and Mullikin,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2018) NISC, NIH, 5625 Fishers Lane, Rockville, MD
            20852, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 214x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/25/2023 04:24:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,345
            CDSs (total)                      :: 6,220
            Genes (coding)                    :: 6,029
            CDSs (with protein)               :: 6,029
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 191
            CDSs (without protein)            :: 191
            Pseudo Genes (ambiguous residues) :: 0 of 191
            Pseudo Genes (frameshifted)       :: 73 of 191
            Pseudo Genes (incomplete)         :: 126 of 191
            Pseudo Genes (internal stop)      :: 29 of 191
            Pseudo Genes (multiple problems)  :: 33 of 191
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP026176.1.
            Bacteria available from Karen Frank, Department of Laboratory
            Medicine, National Institutes of Health Clinical Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..18977
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPNIH50"
                     /db_xref="taxon:573"
                     /plasmid="pKPN-bb08"
                     /geo_loc_name="USA"
                     /lat_lon="38.984652 N 77.094709 W"
                     /collection_date="2016"
     gene            complement(541..963)
                     /locus_tag="C2U50_RS03640"
                     /old_locus_tag="C2U50_03645"
     CDS             complement(541..963)
                     /locus_tag="C2U50_RS03640"
                     /old_locus_tag="C2U50_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714834.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(963..1154)
                     /locus_tag="C2U50_RS03645"
                     /old_locus_tag="C2U50_03650"
     CDS             complement(963..1154)
                     /locus_tag="C2U50_RS03645"
                     /old_locus_tag="C2U50_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1559..1720
                     /locus_tag="C2U50_RS34340"
                     /old_locus_tag="C2U50_03660"
     CDS             1559..1720
                     /locus_tag="C2U50_RS34340"
                     /old_locus_tag="C2U50_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049165871.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPERHDKAPLAAATGNGFRGVGLE
                     VMRLLRLN"
                     /name="hypothetical protein"
     gene            complement(1878..1994)
                     /locus_tag="C2U50_RS03660"
                     /old_locus_tag="C2U50_03665"
     CDS             complement(1878..1994)
                     /locus_tag="C2U50_RS03660"
                     /old_locus_tag="C2U50_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014457.3"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_072072242.1"
                     /translation="MVLAACQVNNVRDTGGGAVSPSSTVTGVTLDSGTNGNP"
                     /name="colicin release lysis protein"
     gene            complement(2027..>3211)
                     /locus_tag="C2U50_RS03665"
                     /old_locus_tag="C2U50_03670"
                     /pseudo=""
     CDS             complement(2027..>3211)
                     /locus_tag="C2U50_RS03665"
                     /old_locus_tag="C2U50_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011191342.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISApu1 family transposase"
                     /name="IS4-like element ISApu1 family transposase"
     gene            complement(3245..6217)
                     /locus_tag="C2U50_RS03670"
                     /old_locus_tag="C2U50_03675"
     CDS             complement(3245..6217)
                     /locus_tag="C2U50_RS03670"
                     /old_locus_tag="C2U50_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_001138073.1"
                     /translation="MPRRSILSATERESLLALPDAKDELIRHYTFNETDLSVIRQRRGA
                     ANRLGFAVQLCYLRFPGTFLGVDEPPFPPLLRMVAAQLKMPVESWSEYGQREQTRREHL
                     VELQTVFGFKPFTMSHYRQAVHTLTELALQTDKGIVLASALVENLRRQSIILPAMNAIE
                     RASAEAITRANRRIYAALTDSLLSPHRQRLDELLKRKDGSKVTWLAWLRQSPAKPNSRH
                     MLEHIERLKSWQALDLPAGIERQVHQNRLLKIAREGGQMTPADLAKFEVQRRYATLVAL
                     AIEGMATVTDEIIDLHDRIIGKLFNAAKNKHQQQFQASGKAINDKVRMYGRIGQALIEA
                     KQSGSDPFAAIEAVMPWDTFAASVTEAQTLARPADFDFLHHIGESYATLRRYAPQFLGV
                     LKLRAAPAAKGVLDAIDMLRGMNSDSARKVPADAPTAFIKPRWAKLVLTDDGIDRRYYE
                     LCALSELKNALRSGDVWVQGSRQFKDFDEYLVPVEKFATLKLASELPLAVATDCDQYLH
                     DRLELLEAQLATVNRMAAANDLPDAIITTASGLKITPLDAAVPDAAQAMIDQTAMLLPH
                     LKITELLMEVDEWTGFTRHFTHLKTSDTAKDKTLLLTTILADAINLGLTKMAESCPGTT
                     YAKLSWLQAWHIRDETYSTALAELVNAQFRQPFAGNWGDGTTSSSDGQNFRTGSKAEST
                     GHINPKYGSSPGRTFYTHISDQYAPFSAKVVNVGIRDSTYVLDGLLYHESDLRIEEHYT
                     DTAGFTDHVFGLMHLLGFRFAPRIRDLGETKLFIPKGDAAYDALKPMISSDRLNIKQIR
                     AHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSV
                     ELRRRVHAGLNKGEARNALARAVFFYRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTV
                     YLERATSALRGNGTALDDTLLQYLSPLGWEHINLTGDYLWRSSAKVGAGKFRPLRPLPP
                     A"
                     /name="Tn3 family transposase"
     gene            complement(6220..6777)
                     /locus_tag="C2U50_RS03675"
                     /old_locus_tag="C2U50_03680"
     CDS             complement(6220..6777)
                     /locus_tag="C2U50_RS03675"
                     /old_locus_tag="C2U50_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236504.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001162012.1"
                     /translation="MQGQRIGYVRVSSFDQNPERQLEGVQVARVFTDKASGKDTQRPEL
                     ERLLAFVREGDTVVVHSMDRLARNLDDLRRIVQGLTQRGVRMEFVKEGLKFTGEDSPMA
                     NLMLSVMGAFAEFERALIRERQREGIVLAKQRGAYRGRKKSLNSEQIAELKRRVAAGDQ
                     KTLVARDFGISRETLYQYLRED"
                     /name="recombinase family protein"
     gene            complement(6907..7119)
                     /locus_tag="C2U50_RS03685"
                     /old_locus_tag="C2U50_03690"
     CDS             complement(6907..7119)
                     /locus_tag="C2U50_RS03685"
                     /old_locus_tag="C2U50_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000993245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="WP_000993245.1"
                     /translation="MNANAPNTASCTTCCVCCKEIPLDAAFTPEGAEYVEHFCGLDCYE
                     RFQARAKAATESDIAPVPGGSQPSD"
                     /name="DUF3330 domain-containing protein"
     gene            complement(7185..7421)
                     /gene="merE"
                     /locus_tag="C2U50_RS03695"
                     /old_locus_tag="C2U50_03700"
     ARG             complement(7189..7421)
                     /name="merE"
                     /gene="merE"
                     /gene_name="broad-spectrum mercury transporter MerE"
                     /tool="amrfinderplus"
                     /drug_class="MERCURY"
                     /identity="96.15"
                     /coverage="100.0"
     gene            complement(7418..7783)
                     /gene="merD"
                     /locus_tag="C2U50_RS03700"
                     /old_locus_tag="C2U50_03705"
     CDS             complement(7418..7783)
                     /gene="merD"
                     /locus_tag="C2U50_RS03700"
                     /old_locus_tag="C2U50_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277466.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]; GO:0006355 -
                     regulation of DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance co-regulator MerD"
                     /protein_id="WP_001277463.1"
                     /translation="MSAYTVSRLALDAGVSVHIVRDYLLRGLLRPVACTTGGYGLFDDT
                     ALQRLRFVRAAFEAGIGLDALARLCRALDAADGDGASAQLAVLRQLVERRREALASLEM
                     QLAAMPTEPAQHAESLP"
                     /name="merD"
     gene            complement(7801..9483)
                     /gene="merA"
                     /locus_tag="C2U50_RS03705"
                     /old_locus_tag="C2U50_03710"
     CDS             complement(7801..9483)
                     /gene="merA"
                     /locus_tag="C2U50_RS03705"
                     /old_locus_tag="C2U50_03710"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281123.1"
                     /GO_function="GO:0016152 - mercury (II) reductase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="WP_000281123.1"
                     /translation="MYLNITGMTCDSCATHVKDALEKVPGVLSALVSYPKGSAQLATDP
                     GTSPEALTAAVAGLGYKATPADAPSTSARGRLLGKALGWLGGGDKAGGDGDGLHVAVIG
                     SGGAAMAAALKAVEQGANVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRESPFDS
                     GIAATVPAIDRSKLLAQQQARVDELRHAKYEGILDSNPAITVLHGEARFKDDQSLAVRL
                     NDGGERVVAFDRCLVATGASPAVPPIPGLKESPYWTSTEALVSDTIPERLAVIGSSVVA
                     LELAQAFARLGSKVTILARSTLFFREDPAIGEAVTAAFRAEGIKVLEYTQASQVAHVDG
                     EFVLTTGYGEIRADQLLVATGRAPNTRSLALEAAGVAANAQGAIVIDKGMRTSTPHIYA
                     AGDCTDQPQFVYVAAAAGTRAAINMTGGDAALDLTAMPAVVFTDPQVATVGYSEAEAHH
                     DGIETDSRTLTLDNVPRALANFDTRGFIKLVIEEGSGRLIGVQAVAPEAGELIQTAVLA
                     IRNRMTVQELADQLFPYLTMVEGLKLAAQTFSKDVKQLSCCAG"
                     /name="merA"
     gene            complement(9522..9929)
                     /gene="merC"
                     /locus_tag="C2U50_RS03710"
                     /old_locus_tag="C2U50_03715"
     CDS             complement(9522..9929)
                     /gene="merC"
                     /locus_tag="C2U50_RS03710"
                     /old_locus_tag="C2U50_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial transporter MerC"
                     /protein_id="WP_000522993.1"
                     /translation="MGLITRIADKAGALGSVVSAMGCAACFPAIASLGAAIGLGFLQEY
                     EGLFISKLLPLFAVVALLANALGWLSHRQWYRSLLGMVGPAIVLAAMLLFFGNWWTANL
                     LYVGLALMIGVSIWDLLSPANRRCELPPKHG"
                     /name="merC"
     gene            complement(9957..10232)
                     /gene="merP"
                     /locus_tag="C2U50_RS03715"
                     /old_locus_tag="C2U50_03720"
     CDS             complement(9957..10232)
                     /gene="merP"
                     /locus_tag="C2U50_RS03715"
                     /old_locus_tag="C2U50_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229640.1"
                     /GO_function="GO:0045340 - mercury ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="WP_000732276.1"
                     /translation="MKKLFAALALAAVVAPVWAATQTVTLSVPGMTCSTCPITVKKAIS
                     KVEGVSKIDVTFETREAVVTFDDAKTSVQKLTKATGDAGYPSSVKQ"
                     /name="merP"
     gene            complement(10248..10598)
                     /locus_tag="C2U50_RS03720"
                     /old_locus_tag="C2U50_03725"
     CDS             complement(10248..10598)
                     /locus_tag="C2U50_RS03720"
                     /old_locus_tag="C2U50_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transport protein MerT"
                     /protein_id="WP_001294660.1"
                     /translation="MSEPQNGRGALFAGGLAAILASACCLGPLVLIALGFSGAWIGNLT
                     VLEPYRPIFIGAALVALFFAWRRIYRPAQACKPGEVCAIPQVRATYKLIFWIVAALVLV
                     ALGFPYVMPFFY"
                     /name="mercuric transport protein MerT"
     gene            10670..11125
                     /gene="merR"
                     /locus_tag="C2U50_RS03725"
                     /old_locus_tag="C2U50_03730"
     CDS             10670..11125
                     /gene="merR"
                     /locus_tag="C2U50_RS03725"
                     /old_locus_tag="C2U50_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166627.1"
                     /GO_function="GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="WP_001166628.1"
                     /translation="MQINFENLTIGVFAKAAGVNVETIRFYQRKGLLPEPDKPYGSIRR
                     YGEADVTRVRFVKSAQRLGFSLDEIAELLRLEDGTHCEEASGLAEHKLKDVREKMADLA
                     RMEAVLSELVCACHARKGNVSCPLIASLQDGTKLAASARGSHGVTTP"
                     /name="merR"
     gene            <11158..11313
                     /locus_tag="C2U50_RS33100"
                     /old_locus_tag="C2U50_03735"
                     /pseudo=""
     CDS             <11158..11313
                     /locus_tag="C2U50_RS33100"
                     /old_locus_tag="C2U50_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /name="DNA-binding protein"
     gene            complement(12432..12623)
                     /locus_tag="C2U50_RS03740"
                     /old_locus_tag="C2U50_03745"
     CDS             complement(12432..12623)
                     /locus_tag="C2U50_RS03740"
                     /old_locus_tag="C2U50_03745"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049106033.1"
                     /translation="MLKKDDIIFMKSCGNAKTIIFLRGVDEKLMINASFEEIKFAINNN
                     LQELPGVQAVAATLKIAT"
                     /name="hypothetical protein"
     gene            12865..13975
                     /locus_tag="C2U50_RS03745"
                     /old_locus_tag="C2U50_03750"
                     /pseudo=""
     CDS             12865..13975
                     /locus_tag="C2U50_RS03745"
                     /old_locus_tag="C2U50_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611476.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(14344..14457)
                     /locus_tag="C2U50_RS34345"
                     /old_locus_tag="C2U50_03755"
     CDS             complement(14344..14457)
                     /locus_tag="C2U50_RS34345"
                     /old_locus_tag="C2U50_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071789001.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            14862..15053
                     /locus_tag="C2U50_RS03760"
                     /old_locus_tag="C2U50_03765"
     CDS             14862..15053
                     /locus_tag="C2U50_RS03760"
                     /old_locus_tag="C2U50_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            15053..>15325
                     /locus_tag="C2U50_RS03765"
                     /old_locus_tag="C2U50_03770"
                     /pseudo=""
     CDS             15053..>15325
                     /locus_tag="C2U50_RS03765"
                     /old_locus_tag="C2U50_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(15359..18256)
                     /locus_tag="C2U50_RS03770"
                     /old_locus_tag="C2U50_03775"
     CDS             complement(15359..18256)
                     /locus_tag="C2U50_RS03770"
                     /old_locus_tag="C2U50_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_001553819.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            18351..18956
                     /locus_tag="C2U50_RS03775"
                     /old_locus_tag="C2U50_03780"
     CDS             18351..18956
                     /locus_tag="C2U50_RS03775"
                     /old_locus_tag="C2U50_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     ARG             complement(10252..10598)
                     /name="merT"
                     /gene="merT"
                     /gene_name="mercuric transport protein MerT"
                     /tool="amrfinderplus"
                     /drug_class="MERCURY"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          1203..1669
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.979"
                     /coverage="92.0"
                     /evalue="2.73e-170"
     Typing          complement(14345..14721)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.825"
                     /coverage="92.0"
                     /evalue="3.51e-178"
ORIGIN
        1 ggaaccgacc cctgatccga aaacggtgaa cggggacgca atttattgcg tggccgtgag
       61 gcgttgccgg cgcagccgga aaaccggttt tcagcacgtt caggaatcgg gttcgtcgac
      121 ggccggcaat cacgttatgg caagtcgccg gacgactcac cacacgattc accggcgatt
      181 cgaatcgtga attgaatcaa gagtcaccat gaacaaaaaa cgcccaaatt gatgaattga
      241 atcaagagtc gattcatcat aacaacatga taataaagcg tgattcacat atgaatcgtg
      301 ggcaattcgt aaacgattcg ccatagagtc gtccacgact tggatctgca ctttgtaaca
      361 catcactgca cattaatttt tcattaacca attgttttaa ataaggaatc tgcaaaattt
      421 taattttgcg tagtgtgtgg gtatttttag tcgtgaggag cgttcagaga tctggcgatt
      481 tttgggaagg ttcaggaact ccctgggatg gccggagagg aaaactcaag catggcagga
      541 tcaggatttt ttccagaatt tccaccacgg ggaatggggt ggtgaagaag gctcgcgctt
      601 gtgctccaga agctgcatag cctgacgaag ggaatcaata tgctcatctc gagccttaac
      661 agccccttta agaccatcgt tttcagcttc aagtagagcg attctatgtt gtaaatcatg
      721 taaaccaacg ctgttaatac ttgtagattc tgcgtcactt tttacatcat attttacagt
      781 atcgatgaca ccatcaagag aaccaaacac tctaataagc tctgaagtat caataacttt
      841 aatagccttc ccgtgcttta caacagaggt gtaacttaac ttcccactgt taatcatctt
      901 gtaaattgtg ggacggctaa ccttaacaag ccgtgccgct tcactaatag acactctggc
      961 catcaattac catcctgtaa ttcatccagt ctcgaggaca gtgtacggaa aagatgctcc
     1021 gcatcatcgt ggagtttctc acaaaggtcg gcctcaacat cgagatcaag ctcgttaagc
     1081 ttctccagca ggagcagaga ctgattctgg atgaatttag ccatgttcag cgctgttttc
     1141 tgttgtttcg tcataaaggg cctcagagcg tcagtgctca atgtcagaca gatctctttt
     1201 ccgcttcctc gctcactcag tcgctacgct cctgccgttc ggctgcggcg agcggtcgca
     1261 acgcgcacat aacgacatag agcagacagc tcggagaggg agaaggcagc acagcggccg
     1321 tttttccata ggctccgccc ccctgacaag catcacagaa tttgacgctc aaatcagtgg
     1381 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctgatgg ctccctcgtg
     1441 cgctctcctg ttcctgccat tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg
     1501 tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat
     1561 gcacgaaccc cccgttcagt ccgactgctg cgtcttatcc ggtaactatc atcttgagtc
     1621 caacccggaa agacacgaca aagcgccact ggcagcagcc actggtaacg gatttagagg
     1681 agttggtctt gaagtcatgc gcctgttaag gctaaactga aagaacaagt tttagtgact
     1741 gcgctcctcc aagccagtta ccttggttca atcacctgca agtgtttgaa ccttcggaaa
     1801 aaccgccctg caaggcggtt ttttcgttta cagagcaaga gattacgacg atccaaatgg
     1861 gatctcaaga agatccttta cggattgccg ttcgtaccgc tatcaagcgt gactccggtc
     1921 accgtcgatg aaggtgaaac agccccacct ccggtgtctc gaacattatt gacctgacat
     1981 gctgccagaa ccacagaaac ggtaacaacc cctcatgtac aggaccttaa gcggcgagtc
     2041 catttaaaac gccttccaag gtcctgttct gtatcgactt tctctgcttg gttcggcagg
     2101 cgttcttggc caaataatcg gcagcccatt ccaatctttc ccttatttct gtcagcgccc
     2161 tatgggccac tgcttccaat agcggtagcc accacgtcgc actgttcttc gacgctttca
     2221 gcatcgatag cgccccactc atcacactct gcgcgacccg tctcttcatc accagcgaca
     2281 gcaggctcgt ccacaccaac ccctcggcaa tcgctttctc gcccgtcaca aaccccttga
     2341 gccggttgtg cgatttccac tctttaaata acaactccac ctgccaacgg cagcgataca
     2401 ggctcatcac ctgctctgct gaccatgctg ctcgcggtag gttcgtcatc cacagacaga
     2461 accgcttttc ctcagcaaac cagcgccgga tcaaccggta ctcgacctgg ccggacttca
     2521 ccaccatatc cagtacctcg gcccgacagc gtcgccgtcc ggcctctctc agagatagtc
     2581 ccgccagctt gggtacttcc cgtccatcac cgcgccaggc ttggattatc ttggggttca
     2641 ggcttttcgt gccgcgcacc agataaaatc cgccagcgtc gttcacctgc tcaaaccaag
     2701 ccctgtcaat gtaacctgcg tcagccagca gcaggcagtt ggtcagcgtg tgggcttcag
     2761 gcagaaattg ccgttcccct gcggtatcgg cagacacact catgcacaac gggctctgct
     2821 ccgacagtga catggtcata tggcactcga tagctgccgg actgatggtc ttgaaccggc
     2881 cgggaaaatc agcagcaagg cgccgatgca ccgcaaagga ggtgccatcg tgcagtaaca
     2941 cctgcttgaa cgcgccgagg gaggctgctt tgagttgatg gtcgatacgc aaggcgatgg
     3001 cccgctcgac cagggctttc ataaacaggg caaagctctc tttgcgcagc tgattgtgaa
     3061 acggtttgta agaaacctga tgggtgttta ccagttgcaa ggcattgaaa tggcggtgta
     3121 aaccggcaat gtcattgacc tggtcgcctc ccatggcccg cagcagtgca ggtacgagca
     3181 tggctggggt aatggcacgc tgacgcagac aggggtcgtc tcagaaaacg gaaaataaag
     3241 cacgctaagc cggtggcagc ggtcgcaatg gcctaaactt ccccgcaccg actttggcgc
     3301 tgctgcgcca taggtaatcg ccggtcaggt tgatgtgctc ccaccccagc ggcgacagat
     3361 attgcaacaa tgtgtcgtcc agcgccgtgc cgttgccacg caaagcactg gtggcacgct
     3421 ccagatatac cgtgttccac aacacgatgg ccgccgtcac cagattgagg ccgctggccc
     3481 ggtagcgctg ctgctcaaaa ctgcggtcgc ggatttcacc caatcggtag aagaagaccg
     3541 ccctggccag cgcgttgcgc gcctcgccct tattcagccc cgcatggacg cggcggcgca
     3601 gctccacgct ttgcagccaa tccaaaatga acagcgtgcg ctcgatgcgc cccagctcgc
     3661 gcaacgccac ggccaagccg ttctggcgcg ggtagctgcc gagtttgcgc agcatcagcg
     3721 aagccgttac cgtgccttgc ttgatggagg tggccagccg cagaatttca tcccaatggg
     3781 cgcgtatttg cttgatgttc agcctgtcgc tgctaatcat cggcttgagc gcgtcatagg
     3841 cggcatcgcc cttggggatg aatagcttgg tttcgcccaa gtcacggata cgcggcgcga
     3901 agcgaaatcc cagcaaatgc atcaagccaa acacgtgatc ggtgaagcct gccgtgtcgg
     3961 tgtagtgttc ctcgatgcgc aagtccgact cgtggtacag caggccatca agcacgtaag
     4021 ttgaatcacg aatgcccacg ttgaccacct tggcactgaa gggcgcgtac tggtcggaga
     4081 tatgggtgta gaaagtccgt cctggactgc ttccatactt cgggttgata tgaccagtgc
     4141 tttctgcttt gctgccggtt ctgaagttct ggccgtccga cgatgacgtg gtgccgtcac
     4201 cccagttgcc ggcgaagggt tgccgaaact gcgcattcac cagctcggcc agcgccgtcg
     4261 aataggtttc atcgcggatg tgccaggctt gcagccaaga cagcttggcg taggtggtgc
     4321 cagggcagga ctcggccatt ttggtcagac ccaggttgat cgcgtcggcc aggatcgtcg
     4381 tcaacagcaa ggttttgtcc ttggccgtgt cgctggtctt caggtgtgtg aagtggcggg
     4441 tgaagcccgt ccattcatcg acctccatca gcaactcggt gattttgagg tgcggcagca
     4501 gcatagctgt ctggtcgatc atggcttgcg cggcgtctgg tactgccgcg tccagcggcg
     4561 tgatcttcag gcctgacgcg gtggtgatga tggcatccgg taagtcgttg gccgcagcca
     4621 tgcggttgac tgtggcgagt tgcgcctcca acaattccaa ccggtcatgc aggtattggt
     4681 cgcagtcggt ggccactgcc agcggcaatt cgctggccag cttcaaagtg gcgaacttct
     4741 cgaccggcac caggtattcg tcgaagtcct tgaactggcg agaaccctgc acccagacat
     4801 caccggagcg cagcgcgttc ttcagctccg acagggcgca taactcgtag taacgccggt
     4861 cgatgccgtc gtcggtcaga accagctttg cccagcgcgg cttgatgaat gcggttggcg
     4921 catcggcggg caccttgcgc gcgctgtcgc tgttcatgcc gcgcagcatg tcgatggcat
     4981 cgagcacacc cttggcggcg ggcgcagccc gcaatttgag cacgcccagg aactgcggcg
     5041 cgtagcggcg tagcgtggca tagctttcac cgatgtggtg caggaaatca aagtcggcag
     5101 gccgcgccaa tgtttgcgct tcggtgacgc tggcggcgaa ggtgtcccag ggcataacgg
     5161 cctcgatggc ggcgaacgga tcgctgccgc tttgcttggc ctcaatcaac gcttgaccga
     5221 tgcgcccata catccgcacc ttgtcgttga tcgccttgcc ggaagcctgg aactgctgct
     5281 gatgcttgtt cttggccgcg ttgaacagct tgccgatgat gcgatcgtga aggtcgatga
     5341 tttcatcggt gacggtggcc atgccttcga tggccagcgc taccagcgtg gcatagcgtc
     5401 gttgcacctc gaactttgcc agatcagcag gcgtcatctg gccaccttca cgagcgattt
     5461 tgagcaggcg gttctggtga acctgccgct cgatgcctgc gggcagatca agtgcttgcc
     5521 aggatttcag gcgctcaata tgttcgagca tgtggcgaga gttcggtttg gcaggcgact
     5581 ggcgcagcca tgccagccac gtcactttac tgccgtcctt gcgcttgaga agttcgtcca
     5641 ggcgctgacg gtggggtgat aacaaagaat cggtcagcgc cgcgtaaatg cgtcggttgg
     5701 cacgggtgat ggcctcggcg cttgcgcgct cgatggcatt catggcgggc aggataatgc
     5761 tctgccgccg cagattctcg acaagtgcgc tcgccagcac gatgcctttg tcggtctgca
     5821 aggccagctc ggtcaatgta tgcacggctt gccgatagtg gctcatggtg aagggcttga
     5881 acccaaaaac cgtttgcagc tcgaccaagt gctcccgccg tgtctgttcg cgctggccgt
     5941 actcgctcca actttccact ggcatcttga gttgcgcggc caccatgcgc aacaggggcg
     6001 gaaacggagg ctcatcgacg cccaaaaagg tgccagggaa tcgcaagtag caaagctgca
     6061 cagcgaagcc caatcgattc gcggcgccgc gacgctgacg gatcaccgac aggtcggttt
     6121 cgttgaacgt gtagtgccgt atcagttcgt ctttggcatc tggcagtgcc agcaggcttt
     6181 cgcgctcggt ggcggacagg attgagcggc gtggcatggt cagtcttccc gcaggtactg
     6241 gtacaaggtt tcgcggctga tgccgaagtc acgggccacc aaggtttttt ggtcgcctgc
     6301 cgcaactcgc cgtttcaact cggcaatttg ttcgctgttc agcgatttct ttcgtccccg
     6361 gtaggcaccg cgctgcttgg ccagcacgat tccctcgcgc tgacgttcgc ggatcagggc
     6421 gcgctcgaac tcagcgaagg ctcccatgac cgacagcatc agattggcca tcggtgagtc
     6481 ctcgccggtg aacttcagcc cttctttgac gaactccatg cgcacgcccc gttgtgtcag
     6541 cccttggacg atgcggcgca ggtcatcaag gttgcgtgcc agcctgtcca tgctatgcac
     6601 caccacggtg tcgccctcgc ggacgaaggc cagcagcctt tccagctcgg gacgctgggt
     6661 gtccttgcca gaagccttgt cggtgaacac ccgcgccacc tgaacaccct ccaattgccg
     6721 ttccgggttc tggtcgaagc tgctgacgcg gacatagccg atgcgttgac cttgcaagat
     6781 gcctccaaag gcaaaagtgt caggatgaaa tctattacct ttgacggaat atgtcaatca
     6841 ataggaaatt taactctatt ctgacatcgt ttgcacatgg catctggcat caagttaggg
     6901 tatgcctcaa tctgacggct gcgaaccgcc agggacaggc gcaatgtcag attctgtcgc
     6961 ggccttggcg cgtgcctgga agcgttcata gcaatccagc ccgcagaaat gttcgacgta
     7021 ttccgcgcct tccggggtga aggcggcatc gagcggaatt tctttgcagc atacgcagca
     7081 ggtggtgcaa ctggcagtgt tcggggcgtt tgcgttcatg gtggtactcc tccagattgg
     7141 tagcgaagct caaagctgcc cactctcggc tggctgggaa gcggtcatga tcttcccttg
     7201 aaggcccgca gcagccgcgt cacagacagg acaaacaagc cggtcagcgt gagggctgca
     7261 ataccccagt gctccccgat gaacgcgccg gccgtcgtgc cggctagcac aatggcgaga
     7321 atcggcaaat ggcagggaca ggtgagcacg gccagcgcgc cccacaagta gccggtgatc
     7381 ggtttgtgcg tctcagacgg caagtgctct gggctgttca tggcagactc tccgcgtgct
     7441 gtgccggttc ggttggcatg gcggccagtt gcatttcgag gctggccagg gcctcgcgcc
     7501 gacgctcgac gagttgccgc aacacggcaa gctgcgcaga cgcaccgtca ccgtccgcag
     7561 catccagcgc ccggcacagc cgcgccagtg cgtccaggcc gatacccgct tcgaaggcag
     7621 cccgtacaaa gcgcagccgt tgcaacgcgg tgtcatcgaa caagccgtag ccgcccgtgg
     7681 tgcacgcgac cggccgtagc aatccgcgca gcaggtagtc gcgcacgata tgcacgctca
     7741 ccccggcatc aagggccagc cgggacactg tgtaggcgct cattgaacac ctccttttcc
     7801 tcatccggcg cagcacgaaa gctgcttcac gtccttgctg aaggtctgcg ccgcgagctt
     7861 cagcccttcg accatggtca ggtaggggaa caattggtcg gccagttcct gcacggtcat
     7921 acggttgcga atggcgagca ccgccgtctg gatcagttca cccgcttccg gggccaccgc
     7981 ttgcacgccg atgagccgtc cgctaccttc ctcgatgacc agcttgatga agccgcgtgt
     8041 gtcgaagttg gcaagcgcac gcggcacgtt gtccaaggtc aaggtgcgac tgtcggtctc
     8101 gatcccgtcg tgatgtgctt ccgcctcgct gtagcccacg gtggcgacct gcgggtcggt
     8161 gaacaccacg gccggcattg cggtcaggtc cagggccgca tcgccgccag tcatgttgat
     8221 cgcagcacgg gtgccggccg ctgccgccac atagacgaac tgaggctggt cggtgcagtc
     8281 gccagccgca taaatgtgtg gcgtactggt gcgcatgccc ttgtcgatga cgatggcccc
     8341 ctgcgcattg gcagcgactc ccgccgcttc caatgccagg ctgcgcgtgt tcggtgccct
     8401 gccagtggcg accagcagct ggtcggcgcg tatttcaccg tacccggtgg tcagcacgaa
     8461 ttcgccgtcc acatgcgcga cctggctggc ttgcgtgtat tccaatacct tgatcccttc
     8521 ggcacggaac gcggctgtaa cggcctcgcc gatggccggg tcttcgcgga agaacagcgt
     8581 gctgcgtgcc aggatcgtca ctttgctgcc cagccgggca aaggcttgcg ccagttccag
     8641 cgccaccaca gacgagccga tcacggccag gcgctcggga atggtgtcgc tgaccagggc
     8701 ctcggtggaa gtccagtagg gtgactcttt caggcccgga atcggcggca cggccgggct
     8761 ggcacccgtg gcgaccaggc agcggtcgaa cgccaccaca cgctcgccgc catcgtttaa
     8821 acggacggca aggctctggt cgtccttgaa acgcgcttca ccatgcagca cggtgatggc
     8881 cgggttgctg tccaggatgc cttcgtactt ggcgtggcgc agctcatcga cgcgcgcctg
     8941 ctgctgggcc agtagtttgc tgcgatcaat cgcaggcaca gtcgccgcga tgccactgtc
     9001 gaatgggctt tcacggcgca aatgggcgat atgggcagcg cggatcatga tcttggacgg
     9061 cacgcagccg acattgacgc aagtaccgcc gatggtgccg cgctcgatca gcgtgacgtt
     9121 cgcaccttgc tcgacggcct tcagcgccgc cgccatcgcg gctccgccgc tgccaataac
     9181 ggcgacgtgc agtccgtccc catcaccacc agccttgtcg ccaccgccca gccatcccag
     9241 cgccttgcca agcagtctgc cccgcgctga agtggatggg gcatcggcgg gtgtggcctt
     9301 gtagccgagg ccggccacag cggcggtcag cgcctctggc gaggtgccgg gatcggtggc
     9361 gagttgcgcg gagcctttcg ggtaggacac gagcgccgac agcacgcccg gcactttctc
     9421 cagggcgtcc ttgacatgtg tcgcgcacga gtcgcaggtc attccggtga tattcaaata
     9481 cattgcatcg ttccttttcg tttcggcaac tgccaatgca gttagccgtg tttgggtggt
     9541 agttcgcagc ggcggttggc cggtgagagc agatcccaga tcgacacccc gatcatcaag
     9601 gccagaccga catagaggag gttcgccgtc caccaattgc caaaaaacaa gagcatggcc
     9661 gccagcacga tggctgggcc gaccatcccg agcaggctgc ggtaccattg ccgatgactc
     9721 agccagccca gtgcattcgc cagcaaagcc acgactgcga acagcggcag cagcttggag
     9781 ataaacaacc cttcgtattc ctgtagaaag ccaaggccga tggccgcgcc caggctggcg
     9841 atagccggga aacaggcagc gcatcccatc gcggaaacaa cgctgccaag cgcgccagcc
     9901 ttgtcggcaa tgcgtgtaat cagtcccatg atcgccccag gttcgggttc agtggctcac
     9961 tgcttgacgc tggacggata gcccgcgtct ccggttgcct tggtcagctt ctgcacgctg
    10021 gtcttggcat catcgaacgt gacaaccgct tcgcgtgtct cgaaggtcac gtcaattttg
    10081 ctgacgcctt cgaccttgga aatcgccttc ttgacggtga tcgggcaggt ggagcaggtc
    10141 atgcccggta cggacagcgt gacggtctgg gtggcggccc acacgggggc aacaacggca
    10201 gcgagggcga gggcggcaaa cagttttttc atgatgaact cctgtgatta atagaaaaat
    10261 ggcatgacgt agggaaatcc gagcgcgacc aggaccagcg cggccacgat ccagaaaatg
    10321 agcttgtaag tagctcgcac ttggggaatc gcgcagacct cacccggttt gcaggcttgc
    10381 gccgggcggt agatgcgccg ccaggcgaaa aacagcgcga ccagcgctgc gccgatgaag
    10441 atcgggcgat agggttccag caccgtcagg ttgccgatcc atgccccgct gaaccccaag
    10501 gcgatcaaaa ccagcggccc caggcagcag gccgacgcaa gaatggcggc cagcccaccg
    10561 gcgaagagcg cgccgcgccc gttttgtggt tcagacatac gcttgtcctt tcaaatttgg
    10621 tttggatagc ttaagcttac ttccgtagtt atgtacggag tcaagcgata tgcaaattaa
    10681 ttttgagaat ctgaccattg gcgtttttgc caaggcggcc ggggtcaatg tggagaccat
    10741 ccggttctac cagcgcaagg gcctgctgcc ggagccagac aagccctatg gcagcattcg
    10801 ccgctatggc gaggcggatg taacacgagt gcggttcgtg aaatcggccc agcggctggg
    10861 ctttagcctg gacgaaatcg ccgagctact gcggctggag gatggcaccc attgcgagga
    10921 agccagcggc ctggccgagc acaagctcaa ggatgtgcgc gagaagatgg ccgacttggc
    10981 acgcatggag gccgtgctgt ctgaactggt gtgcgcctgc catgcgcgga aagggaacgt
    11041 ttcctgcccg ctgattgcgt cactgcaaga cggaacgaag ctcgctgcat cggcgcgggg
    11101 gagtcacggg gtgactacgc cttagcgtgc tttattttcc gaattctgag acgaccccta
    11161 cttgaagctg aaaacgatgg tcttaaaggg gctgttaagg ctcgagatga gcatattgat
    11221 tcccttcgtc aggctatgca gcttctggag cacaagcgcg agccttcttc accaccccat
    11281 tccccgtggt ggaaattctg gaaaaaatcc tgatcctgcc atgcttgagt tttcctctcc
    11341 ggccatccca gggagttcct gaaccttccc aaaaatcgcc agatctctga acgctcctca
    11401 cgactaaaaa tacccacaca ctacgcaaaa ttaaaatttt gcagattcct tatttaaaac
    11461 aattggttaa tgaaaaatta atgtgcagtg atgtgttaca aagtgcagat ccaagtcgtg
    11521 gacgactcta tggcgaatcg tttacgaatt gcccacgatt catatgtgaa tcacgcttta
    11581 ttatcatgtt gttatgatga atcgactctt gattcaattc atcaatttgg gcgttttttg
    11641 ttcatggtga ctcttgattc aattcacgat tcgaatcgcc ggtgaatcgt gtggtgagtc
    11701 gtccggcgac ttgccataac gtgattgccg gccgtcgacg aacccgattc ctgaacgtgc
    11761 tgaaaaccgg ttttccggct gcgccggcaa cgcctcacgg ccacgcaata aattgcgtcc
    11821 ccgttcaccg ttttcggatc agaagaacgc cggaaacagg acttaccctg gctgaatggc
    11881 gtgaatttcc gcgctatgca cttgcgcgca tactcatgca tgccgtaaaa acagagcctg
    11941 cgcgtttctg gcgggttttc gggtggtttg ttgcctgttt taccggtttc ccgtcagaaa
    12001 cgccctgagg ccgttttagc ggtgcgcgta agaagacgtt atggtaaatc caaaaatgct
    12061 ggcattcgca attcagtata tcggtacgca gcataataat gggggttaat ttccaacgta
    12121 tgagccatca ggtgctaagg ttgcttccga accaccaacg tatgagccat caggtgctaa
    12181 ggttgcttcc gaaccactaa cgtacgatcc atcaggtgct aaggttactt gggatatcgc
    12241 ggttagtggt aatgctgaca tagccattaa aacggcacca agagataatg ctctcatcac
    12301 tattactcct gttaatatca aaaatagaat tgaatcgttc agcggtgccg aacgccggtt
    12361 ccagccgcgg gattcgtgat cgggatgtta tagtaaattg atctcttgat tttgagtgcc
    12421 cttactcgaa atcaggttgc tattttcagt gtcgcggcta ctgcttgtac ccccggaagc
    12481 tcctgaagat tattattgat agcaaacttt atctcttcaa agctcgcgtt aatcatgagc
    12541 ttctcatcca cgccacgtaa aaatattatt gtttttgcat taccacatga tttcataaag
    12601 ataatatcat ctttttttag catgatactg tcgttactta gtgtagtgag ataaagcata
    12661 gccactgttt ttgtgtgttt ttgttaaggc tgttaagatt attcctaacc actgattttg
    12721 tacatatcat caaaaaaaat aaaaaaacta atgcccccgt ggtgggtata taacaaatca
    12781 cggggagcgg agctgacctg ctccccgttg attaatacac cgcgatgtta gtaatgtctt
    12841 cataagccac atgaggacat cctcatgaag aagcgttttt ccgacgaaca gatcatcagt
    12901 attctccgcg aggccgaagc cggggtttct gcccgtgagc tctgccgtaa gcacgccatt
    12961 tccgacgcca tcttttacac ctggcgtaag aagtatggcg gtatggaggt gcccgaggtt
    13021 aagcgcctta agtcgcttga ggaagagaac gccagactca agaaactgct tgccgaagcc
    13081 atgctggata agcaggcgct tcaggtggct ctggggcgaa agtactgacg acagaccaga
    13141 agcaggaagc cgttgagttt atgtgtgatg cgaccggtct gtcgcaacgt agtgcctgca
    13201 ggcttacagg tttgcccctg tcgacctgcc gctatgaggc tcagcgttcg gcggctgatg
    13261 cgcatttatc acggagctgg cactggagcg caggcgtttt ggctaccgac gcatctggca
    13321 gttactgcgc cgtgaaggcc ttcatgttaa tcacaagcgc gtgtaccgtc tttaccacct
    13381 taacgggctg ggtgtaaaac gcagacgacg tcgtaaaggt ctggcaacag aacgtctgcc
    13441 gctgctccgc ccggaggcgc ccaacctgac ctggccgatg gattttgtca tggacgcgct
    13501 ggccaccggt cgcaggatca agtgcctgac ctgcgtggac gacttcacga aggagtgtct
    13561 gacaattacc gccgtcttcg ggatttcagg cgttcaggtc acgcgaattc tggacagcat
    13621 tgcactgttt cgcggctatc cggcgacgat aagaactgac caggggccgg agtttacctg
    13681 cagagcactc gaccagtggg cttatgagca tggggtggag ctacggctta tccagccggg
    13741 caggccaaca cagaacggat ttattgaaag ttttaacgga cgattcaggg atgagtgcct
    13801 caatgagcac tggttcagcg atatcgttca cgccaggaaa acgattaatg actggtggca
    13861 ggattagagc gagtgtcgtc cacattcatc gctgaactac cagactccgg ctgaatttgc
    13921 ggcgggctgg cgaaacggga aatatgaaga aaaacgaacc gacattacta actgaaggtt
    13981 gtatctaatc ctggggggca ggtcaaccgc gttggatgat tgtggatcat gattatatat
    14041 gttttttctt atcagaaaca atgggttaaa tagttttatt ttgatggttg gtgtcgcgga
    14101 gtggtgctgg tttcacttca ggggtaggca tatggaacag gatcctcttg agatcctttc
    14161 tttagcatcg taatctcttg ctcagtaaac gaaaaaaccg ccctgcaagg cggttttttc
    14221 gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag gagcacagtc
    14281 accaaaactg ttcgtttagt ttagccttaa ccggcgcata acttcaagtc catctcctct
    14341 aaatcagtta ccagtggctg ctgccagtgg cgcttttgca tgtcttaccg ggttggactc
    14401 aagacgatag ttaccggata aggcgcagca gtcggactga acggggggtt cgtgcataca
    14461 gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacaaac
    14521 gcggccataa cagcggaatg acaccggtaa accgaatggc aggaacagga gagcgcacga
    14581 gggagccatc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact
    14641 gatttgagcg tcaaattctg tgatgcttgt caggggggcg gagcctatgg aaaaacggcc
    14701 gctgtgctgc cttctccctc tccgagctgt ctgctctatg tcgttatgtg cgcgttgcga
    14761 ccgctcgccg cagccgaacg gcaggagcgt agcgactgag tgagcgagga agcggaaaag
    14821 agatctgtct gacattgagc actgacgctc tgaggccctt tatgacgaaa caacagaaaa
    14881 cagcgctgaa catggctaaa ttcatccaga atcagtctct gctcctgctg gagaagctta
    14941 acgagcttga tctcgatgtt gaggccgacc tttgtgagaa actccacgat gatgcggagc
    15001 atcttttccg tacactgtcc tcgagactgg atgaattaca ggatggtaat tgatggccag
    15061 agtgtctatt agtgaagcgg cacggcttgt taaggttagc cgtcccacaa tttacaagat
    15121 gattaacagt gggaagttaa gttacacctc tgttgtaaag cacgggaagg ctattaaagt
    15181 tattgatact tcagagctta ttagagtgtt tggttctctt gatggtgtca tcgatactgt
    15241 aaaatatgat gtaaaaagtg acgcagaatc tacaagtatt aacagcgttg gtttacatga
    15301 tttacaacat agaatcgctc tacttggggt cggttccggc tgagggcgaa atgacaccct
    15361 aagctttcgg ttccttgggc caaagatatt cgccagtcag tagaatgtgc gcccagccca
    15421 atggggatat gtggggaaga aattcagggg gaacatccaa cccttcgttc cgccgctccg
    15481 tgacggcatg accaagatgg acggtattcc agtaaatgat caccgcagtc aataaattga
    15541 gcccagcgat tcggtagtgc tgcccctctg tcgtgcgatc gcgaatttcc ccctgcctcc
    15601 cgatacggag cgcatttttg agcgcatggt gggcctctcc cttgttaaga ccgatctgag
    15661 cacgccgctg catgtccgta tccaggatcc actcaataat gaaaagggtc cgttcaatac
    15721 gaccaacttc acgaagcgca actgcaaggt tgttttgtcg tgggtaagaa gcgagcttgc
    15781 gcaggagttg gctgggcctg attttgccag cggtcatcgt cgcggcacaa cggaaaatat
    15841 caggccagtt cgcaacgata agatcctccc gggcttttcc acctaccaac ttgcgtaact
    15901 ccctgggggt cgtatcggga ttaaatacgt acaaccgctt cgatggcaga tccctgattc
    15961 gcagaacgag attgtagccg agcaggctac tggctccgaa caaatggtcg gtgaatcctg
    16021 ctgtatcggc atactgttcg cgaacatggc gaccgacctc gttcatcagt agtccatcga
    16081 gaatatacgg tgcctcgctc acggtcgccg ggatcgactg acaagcgaat ggcgcgaact
    16141 ggtcgcttac gtgagtatac gctttgaggc cgggaacaga accatatttg gcattgacca
    16201 tgttcatggc ttcgccatgc cgcgctgtcg ggaaaaactg accatcgctc gatgctgacg
    16261 tgcccatccc ccagacgcgt gacatcggca gtttaccctg cgcggccacc acaattgcca
    16321 atgcctggtt catggcttcg ctttcaacat gccagcgggc aaggcgtgag agctgccagt
    16381 aatcatgcgt gtttgtagct tccgccatct tacgcaggcc cagattgagc ccttcagcga
    16441 gcaggacgtt gagcagaccg atccggtcgc gacatggagc cccggttctc agatgggtaa
    16501 acgcatctgt gaaaccaagg gctgcatcaa cttcaagcag catgtcggta atccgaacgg
    16561 acggcattcg gcgatacaga tccagtatga gtgcctcggc accatccggc acgtctgctg
    16621 tcaacctgtc gatccgcaac gttccatctt ctatgctacc gtgcggaata gtgccgttac
    16681 gggcagcccg ggccagccgc ttaagagcga tcgtgagtcg cgcctttctg tctgccagcc
    16741 aatcctgtgg gttggaaggc acggccagtt ttgcattttc ctgcgccgcg atcatcggca
    16801 ccagtacctg cttgaggtca ccatagcggc gcgaatgagc gagccagaca tctccggaac
    16861 gaaaagcatc ccggaggtga aagagtaccg ccacttccca aagacgggta tctccttttt
    16921 cctgagctcg taaatgacgg ttccatttgg agctgggccg caggaaacgc ctttctggcg
    16981 atgcaacacc tttcatctct ccgatcgaca aagctgctgc taccaatggt ccggcgaccg
    17041 gcgcggcttc gagcttcaga cagcgcaaca tgcggggcgc ataacgacga aagcgatggt
    17101 atccctgccc gacatatgca agaggctcat cggctagcgt gttgctgagt tgagtccctg
    17161 tcgctaccag ttgagcgagc cggtcccatg caaccgaact ggcgacagcc atctccagcg
    17221 gggttccgtc actgcgggcc tcaagcaacg aagctcccag cgcggtgaag gtacggatcg
    17281 tatccgtgag tgtggcttta gagccggaaa ttgtttcgtc atgctggcgc ttcgcttccc
    17341 gccaggtttt tcctacgatc ctgtcatggg tttcgactat ggcatcagca atcgccgctt
    17401 cccactccac aacacagacg gcaaggatcg cccagcggcg gtccgaagtg atgtcacgca
    17461 aaccgtcggt gaagtagcgt tcaccctgcc gacgcagccg ggcaatgcga tgggcaggta
    17521 tgctggccaa agcactatga ttgatattca gggtacgcag aaattcgagc ctgtcgagca
    17581 aacggttagc agcagccgag ttgttaccaa cctcgaagtt gcgaagccag atgaaacgac
    17641 tgatattgcc ggcgagcatt tcactcagaa gtttgtccag gtgatcgcga acatccgctg
    17701 ttaaattttc cgcaatccgc gtttcaatcc gccgctcagc ggcgaccaga gcatccgcgc
    17761 acaagcgctc gattgtcgat actgcgggca gaatggtgga agtttcccga caccgcacaa
    17821 taaaacgatg agcaagatcc tcgtttgatc tggcatcttc ggcctggccg aaagtccact
    17881 cccgcagatc acgggcacca cggcccgtga aggtcttgta gccgtaaatt tcgcgcagcg
    17941 tgtccatgtg ctgctgacgg gtttggcgcc gtgtggcata agtgagaagc gcatcagccg
    18001 gaactccaag ctgagcaccg acgaaggaaa ggacttcacg cgggatcatc tcaccaggag
    18061 ccagtgcacg gcccggatat cgtaaggcac aaagttgcag ggcaaagcca atcctgtttt
    18121 ccggtctgcg gcgctgccta atgttttcca ggtcatcatc gcccagcgtg tagaacttca
    18181 gtagcgacag ttcgtccgtg ggcagatcga acagcgctgc tcgctgccgt tcggtgaaaa
    18241 tatggcgtcg tgacatacaa attcgtccct tttgaagtat agtctgtttt ggacaacagc
    18301 cagcccatat aaatcagggc gttccgatac aaaaatccag gagggttcaa ttgggacatc
    18361 gtgccgccat ttactgccgg gtttcaacag cggatcagtc ttgtgaacgc caggaatttg
    18421 atctgcgagc cttcgccggc cgtgccggct acgacgtggt gggaatattt aaggaaacag
    18481 gttcaggaac taaactcgac cgggccgagc gaaagaaagt cctggcgctt gcccagtcca
    18541 gacaaattga tgcaatcctg gtcactgagc tttcccggtg ggggcgctcg acgctcgatc
    18601 tgctcaatac gctacgtgaa ctggagaact ggaaggtttc cgtgatagcc atgaatggaa
    18661 tggcgttcga tctttcgtcg ccgtatggac gaatgctggc gacgtttctt tccggcattg
    18721 cggagtttga gcgggatctc atcagcgagc gggtcaagtc aggccttgct gttgcgaagg
    18781 cacgtggtaa gaggcttggt cgtcaggccg gagtgcgacc aaaatcagac cgacttttgc
    18841 ctaaggtggt tgcgatgagg gccgagggac gcagctatcg ctggatcgca cgcgagctcg
    18901 gtatcagcaa gaataccgtc gctgacatcg tgcaacgaca cagagctaac gcttagggtg
    18961 tcatttcgcc ctcagcc
//
