LOCUS       NZ_CP026126            11305 bp    DNA     circular CON 26-DEC-2023
DEFINITION  Acinetobacter baumannii strain ABNIH28 plasmid pABA-6973, complete
            sequence.
ACCESSION   NZ_CP026126
VERSION     NZ_CP026126.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06040401
            Assembly: GCF_002902885.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11305)
  AUTHORS   Weingarten,R.A., Johnson,R.C., Conlan,S., Ramsburg,A.M.,
            Dekker,J.P., Lau,A.F., Khil,P., Odom,R.T., Deming,C., Park,M.,
            Thomas,P.J., Henderson,D.K., Palmore,T.N., Segre,J.A. and Frank,K.M.
  CONSRTM   NISC Comparative Sequencing Program
  TITLE     Genomic Analysis of Hospital Plumbing Reveals Diverse Reservoir of
            Bacterial Plasmids Conferring Carbapenem Resistance
  JOURNAL   mBio 9 (1) (2018) In press
REFERENCE   2  (bases 1 to 11305)
  AUTHORS   Segre,J.A. and Mullikin,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2018) NISC, NIH, 5625 Fishers Lane, Rockville, MD
            20852, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 221x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/26/2023 04:39:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,009
            CDSs (total)                      :: 3,913
            Genes (coding)                    :: 3,746
            CDSs (with protein)               :: 3,746
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 167
            CDSs (without protein)            :: 167
            Pseudo Genes (ambiguous residues) :: 0 of 167
            Pseudo Genes (frameshifted)       :: 49 of 167
            Pseudo Genes (incomplete)         :: 122 of 167
            Pseudo Genes (internal stop)      :: 30 of 167
            Pseudo Genes (multiple problems)  :: 27 of 167
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP026126.1.
            Bacteria available from Karen Frank, Department of Laboratory
            Medicine, National Institutes of Health Clinical Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11305
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="ABNIH28"
                     /db_xref="taxon:470"
                     /plasmid="pABA-6973"
                     /geo_loc_name="USA"
                     /lat_lon="38.984652 N 77.094709 W"
                     /collection_date="2016"
     gene            complement(join(11182..11305,1..233))
                     /locus_tag="C2U32_RS18590"
                     /old_locus_tag="C2U32_18580"
     CDS             complement(join(11182..11305,1..233))
                     /locus_tag="C2U32_RS18590"
                     /old_locus_tag="C2U32_18580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269904.1"
                     /translation="MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSNKKRFYTEMLAIADEQ
                     YALHLSTLGDQSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(771..1238)
                     /locus_tag="C2U32_RS18595"
                     /old_locus_tag="C2U32_18585"
     CDS             complement(771..1238)
                     /locus_tag="C2U32_RS18595"
                     /old_locus_tag="C2U32_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004727917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="WP_079393076.1"
                     /translation="MIEKGAIISDCNKYRYSLWRIWDKEKPIFTFVGLNPSTADHQQDD
                     RTIRRCINFAKSWGGGGIYMANLFAYRATDPQEMMSQEDPIGPDNDAHLKQLAAESHKL
                     IACWGNDGVFKARSTFVKQLLTDKLYYLKLNKSGEPGHPLYIHSSTQIQPF"
                     /name="DUF1643 domain-containing protein"
     gene            1425..1943
                     /locus_tag="C2U32_RS18600"
                     /old_locus_tag="C2U32_18590"
     CDS             1425..1943
                     /locus_tag="C2U32_RS18600"
                     /old_locus_tag="C2U32_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447196.1"
                     /translation="METFAEPVFSNDLLAKAESGDTSAQLELAEIYLYGHGVDSDENQA
                     EIWAIKSAENGNVAAMFWLADGYVTYARLIEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNIKKALELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            1982..>2257
                     /locus_tag="C2U32_RS18605"
                     /old_locus_tag="C2U32_18595"
                     /pseudo=""
     CDS             1982..>2257
                     /locus_tag="C2U32_RS18605"
                     /old_locus_tag="C2U32_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019767798.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /name="SMI1/KNR4 family protein"
     gene            complement(2294..3517)
                     /locus_tag="C2U32_RS18610"
                     /old_locus_tag="C2U32_18600"
     CDS             complement(2294..3517)
                     /locus_tag="C2U32_RS18610"
                     /old_locus_tag="C2U32_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343018.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISAba26 family transposase"
                     /protein_id="WP_004738162.1"
                     /translation="MDEATIKSMAAELAKGLKTPEDLNQMTAVFKKFMIETALNTELSD
                     HLGYEKHQPKKGSNSRNGFSSKTITTQDGQLALDIPRDREGSFEPQIIKKHQTRITSMD
                     DQILSLYAKGMTNREIVAFFKEMYDADVSASLISKVTDAVIEQVTEWQNRALDSLYPVV
                     YLDCIVVKVRQHSNVINKSVYLALGINMDGQKELLGMWIAQTEGAKFWLSVMTELKNRG
                     VQDILVACLAKQRFSGLKGFPDAIASVYPHTDIQLCIVHVVRNSLRFVSWKDYKAVTSG
                     LKAIYQASTEENALKSLDIFCDQWNHQYPKIGESWRANWENIRTIFSYPAEIRHAIYTT
                     NAIESLNSVIRHSTKKRKIFSSDDSVKKVIYLATSNAAKKWTMPIQNWRLAMNWFTIQF
                     DDRLKDHL"
                     /name="IS256-like element ISAba26 family transposase"
     gene            <3593..3835
                     /locus_tag="C2U32_RS18615"
                     /old_locus_tag="C2U32_18605"
                     /pseudo=""
     CDS             <3593..3835
                     /locus_tag="C2U32_RS18615"
                     /old_locus_tag="C2U32_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019767798.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /name="SMI1/KNR4 family protein"
     gene            4018..4446
                     /locus_tag="C2U32_RS18620"
                     /old_locus_tag="C2U32_18610"
     CDS             4018..4446
                     /locus_tag="C2U32_RS18620"
                     /old_locus_tag="C2U32_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016653412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016653412.1"
                     /translation="MKYSNEKIVKALLLSPLPLLFFTAVLFIVMNQEYSLYSILVVLVG
                     HGLVYLAYCILTVPFSFIFSILLNRYNSLNLLTICIASIIIATPFFILFGWSHTGAISK
                     EWWKMYTDTWTIFMALFPGLCYWLFLINLKDKKSKNIE"
                     /name="hypothetical protein"
     gene            complement(4512..4703)
                     /locus_tag="C2U32_RS18625"
                     /old_locus_tag="C2U32_18615"
     CDS             complement(4512..4703)
                     /locus_tag="C2U32_RS18625"
                     /old_locus_tag="C2U32_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102616.1"
                     /translation="MSTELVNSANIISFPKPCAFCESTEHVQLFAGLMLCRSCQENIKI
                     TNPDLFATNDQIQQKAQD"
                     /name="hypothetical protein"
     gene            complement(5166..5384)
                     /locus_tag="C2U32_RS20680"
                     /old_locus_tag="C2U32_18620"
     CDS             complement(5166..5384)
                     /locus_tag="C2U32_RS20680"
                     /old_locus_tag="C2U32_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_029424581.1"
                     /translation="MNGQKMIFLRFSKPRNKYFFLYKTKHQSYVTLANFLIKDPAHAGI
                     FLSHTTHLKMITLKPLLDMVFIGFFII"
                     /name="hypothetical protein"
     gene            6113..7042
                     /gene="repM"
                     /locus_tag="C2U32_RS18635"
                     /old_locus_tag="C2U32_18625"
     CDS             6113..7042
                     /gene="repM"
                     /locus_tag="C2U32_RS18635"
                     /old_locus_tag="C2U32_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005133531.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLRDASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YEPQSGIVFLRFTQDIVPLITRLEENFTKYELEQVSRLTSSYAIRLYELLIQWRSAGKT
                     PIFDLSIFRQQLGVEAHQYKTMSNFKTYVLDFALKQVNELTDVKAKYEQHKKGRSISGF
                     SFSFKQKKTNSDKVIKGTDTLALFTKMSDKQRHLFANKLSELPEMGQYSEGTESFQQFA
                     IRIAEMLQDYQKFQELFPYLQKVGYQAA"
                     /name="repM"
     gene            7121..7645
                     /locus_tag="C2U32_RS18640"
                     /old_locus_tag="C2U32_18630"
     CDS             7121..7645
                     /locus_tag="C2U32_RS18640"
                     /old_locus_tag="C2U32_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008307689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_005127114.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSDNKIDLAEAIRV
                     FGEPSKKQDVKESVKIDSPISTEVLLLRQQINMLQNQLDDAKERESFYQNQIETMQRLL
                     EAPKPEIQEPSEPELPQSAPIVEMKEQVIVVPPKDDGLTTPENKRIPVPEHVEPEPKKR
                     GFWSRFFLPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            7983..8300
                     /locus_tag="C2U32_RS18645"
                     /old_locus_tag="C2U32_18635"
     CDS             7983..8300
                     /locus_tag="C2U32_RS18645"
                     /old_locus_tag="C2U32_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005065053.1"
                     /translation="MLGENHHFLKKFSISDAAIAYKKPRSTILKYIKSGDFELDYNKLI
                     EYSQLVRKFGELTYPIQDEREQQIKKLKDEVMHLKKQLNFFKYTSISNKNRLLISHKHS
                     I"
                     /name="hypothetical protein"
     gene            complement(8774..9046)
                     /gene="nadS"
                     /locus_tag="C2U32_RS18650"
                     /old_locus_tag="C2U32_18640"
     CDS             complement(8774..9046)
                     /gene="nadS"
                     /locus_tag="C2U32_RS18650"
                     /old_locus_tag="C2U32_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_000369780.1"
                     /translation="MDNNLFDDLVASIKEAGAIKRKEIKASRVTELELPDIKEVREKTG
                     LTQAEFAARLHISARTLQNWEQGRRYPTGPAATLIRILDAHPTLI"
                     /name="nadS"
     gene            complement(9039..9359)
                     /locus_tag="C2U32_RS18655"
                     /old_locus_tag="C2U32_18645"
     CDS             complement(9039..9359)
                     /locus_tag="C2U32_RS18655"
                     /old_locus_tag="C2U32_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000897309.1"
                     /translation="MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGDLIKNGGGI
                     RKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAYPKSVKDNLTDKETSILRQLVKEQF
                     HG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(9546..9743)
                     /locus_tag="C2U32_RS18660"
                     /old_locus_tag="C2U32_18650"
     CDS             complement(9546..9743)
                     /locus_tag="C2U32_RS18660"
                     /old_locus_tag="C2U32_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000476222.1"
                     /translation="MFKQTLSVKDQFGVKHAIQATVDHEFANTQSHLNIKHITVDGEDI
                     RPSFEMLFQSTLSGKIFKII"
                     /name="hypothetical protein"
     gene            complement(9810..10037)
                     /locus_tag="C2U32_RS18665"
                     /old_locus_tag="C2U32_18655"
     CDS             complement(9810..10037)
                     /locus_tag="C2U32_RS18665"
                     /old_locus_tag="C2U32_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000921865.1"
                     /translation="MLKVNDLIAKSKNGTEIIVSLIPLHKMQNTRQGLKQIEVGKRVLL
                     ESGIEVDLNLDGRTFYTSLNQLFKLNHKVA"
                     /name="hypothetical protein"
     gene            complement(10135..10350)
                     /locus_tag="C2U32_RS18670"
                     /old_locus_tag="C2U32_18660"
     CDS             complement(10135..10350)
                     /locus_tag="C2U32_RS18670"
                     /old_locus_tag="C2U32_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004693381.1"
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]; GO:0003723 - RNA binding [Evidence IEA]; GO:0001072 -
                     transcription antitermination factor activity, RNA binding
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="WP_000071892.1"
                     /translation="MSNSNVVKGTVKWFNETKGFGFIQQESGPDVFAHFSEIASSGFKT
                     LFEGQQVEFSVAQGQKGPNAVNIVAI"
                     /name="cold-shock protein"
     gene            complement(10685..10894)
                     /locus_tag="C2U32_RS18675"
                     /old_locus_tag="C2U32_18665"
     CDS             complement(10685..10894)
                     /locus_tag="C2U32_RS18675"
                     /old_locus_tag="C2U32_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069475.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCEYFS
                     HCKRPSYYIDIFDNAYWLKYYEIA"
                     /name="hypothetical protein"
     gene            complement(10887..11189)
                     /locus_tag="C2U32_RS18680"
                     /old_locus_tag="C2U32_18670"
     CDS             complement(10887..11189)
                     /locus_tag="C2U32_RS18680"
                     /old_locus_tag="C2U32_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001129974.1"
                     /translation="MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     Typing          6114..7038
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.684"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tgtcttaggg gatcgaatgc aaaaaatgcc cttaacggtt taccacgatg ctgaacacgt
       61 agttccttca tattggtaaa tttagaatcg tagacggtat ctactaaagg acgacctaaa
      121 ctaggccctt gctgttgtag aacaaccaat gcagctaaga ctttctcttg tgttgcttca
      181 tcttgctgtt caagccattc attaaacaga tccgttgtaa tgactgtcca catattacaa
      241 tcacaatata gattacagtc tatatattaa atctgattga atgactttgc aaatggcaat
      301 aaaaaatccc gacgtcatgt cgggattttt cggtatggag cgcataggta gggctgacta
      361 cctgatggtc atggtgcctg actgattatt attgttgttt taagtcattg aacatcctgt
      421 ccatatagat cgatcataag agcttcatcg ggggatagat acagacaatt tcgtgaaacg
      481 ctgtaagcca aaacgtacaa aaaataatca aaatttatcc cagtggttat ccacagaaat
      541 agtggattct tttgggtaaa aaagcttata aactctatgg aattgctatt tttgattatt
      601 ttttaaacaa ttttattggc aagaagtatc aaaattgaac ataaaaaaac cgatttattt
      661 ctaaatcggt tgaacgagat cgttccaggg gtttaactca atgtaagtta ttgatcttaa
      721 agtaaactta aagggccatt tcgcataagc accattatgt taaatgggat ctaaaaaggc
      781 tgtatctgag tggaagaatg tatatataac ggatgtccag gttctccact tttatttaat
      841 ttaagataat agagtttgtc cgtaagcaat tgttttacaa aagttgatct cgctttaaaa
      901 acaccatcat ttccccaaca agcaataagt ttgtgggatt ctgctgctaa ttgctttaag
      961 tgggcatcgt tatcaggacc aatagggtct tcttgggaca tcatttcttg tggatcagta
     1021 gctctatatg caaatagatt agccatataa atgccaccac ctccccaact tttagcaaaa
     1081 ttaatgcatc gtcttatggt tcggtcatct tgctgatggt ctgcagttga tggatttaag
     1141 ccgacaaagg taaatattgg tttttcttta tcccaaatac gccaaagtga ataacgatat
     1201 ttgttacagt cggaaatgat tgcccctttt tctatcacta cattgtcctt cttgatctat
     1261 ttcgaataag agtttctatg ttaaatagga ctaaaaatat actctaattt tagagttttc
     1321 ttttaggtat gatgtaaaaa catacaagcc taagagttta atttaaaggt taagaattga
     1381 ataaattagt aaatattttt atggctttag gagtttgcct ttcaatggaa acatttgcag
     1441 aacctgtttt ttctaatgat ttattagcga aagcagaaag tggagatact tctgcccagt
     1501 tagaattagc tgagatttat ctatatggtc atggtgttga ttcagatgaa aatcaagctg
     1561 aaatttgggc tattaaatca gctgaaaatg gaaatgtagc agcaatgttt tggttagctg
     1621 atggatatgt tacctatgct agattaatag aggatgatga caaaaacgat tctttagaac
     1681 atttccaaaa agcttttaag tggtttcaga aagcttcaga aaatggtcat tctgaatcaa
     1741 tggttgagtt agctgaccta tatactcgcg cagatagcgg aatagaagtt aatattaaga
     1801 aagctttaga acttcgtgaa aaggctgcaa agttaggtaa taagaaagca atgcgaagtc
     1861 tttccgttat gtatcgtgat ggtataggca ttcctaaaaa tactgattta gctcaaagtt
     1921 ggtgggataa gtctgaaaat taattttagt aaaagttaag gattttttaa gagtctaata
     1981 aatgaatagt gttcatgaaa ttatagaaaa aatacataat gaatgggaga ttgaacctaa
     2041 gaaagctatt cagagaggta gggaatgtcc ctttcctttg cagtgttcat taaacctaaa
     2101 aagcaaaatt tacccacaaa taccccaagt acttttacca aaagtattag aggattttta
     2161 tacagtatct aatggtgcag atctatttaa agatcaagaa tacggacaat ggggcttaaa
     2221 attatattct attgaggaag taatttttgc ttcaaaaatg agcctgtaaa ttattttgtg
     2281 taagttccat tttttataaa tgatctttta atcgatcatc gaactgaatc gtaaaccaat
     2341 tcattgctaa acgccaattt tgaattggca tcgtccattt cttcgcagca tttgatgttg
     2401 ctaagtaaat gaccttcttt actgagtcat cagatgaaaa gattttcctt ttcttcgttg
     2461 aatggcgtat tacgctattc aacgactcaa tcgcatttgt tgtataaatc gcatgacgta
     2521 tttcggctgg atagctaaag atcgttcgga tattttccca attggcccgc caggattctc
     2581 caattttggg atactggtga ttccattgat cacagaagat gtctagggac tttaaagcat
     2641 tttcctctgt acttgcctga taaatcgctt tcagacccga cgtaacagcc ttgtagtctt
     2701 tccagcttac aaatctcagg ctattgcgta caacatgcac aatacacagt tgaatatcag
     2761 tatgagggta aacagaggct atcgcgtcag ggaagccttt taatcctgag aagcgctgct
     2821 tcgcaaggca ggcaacaaga atgtcctgta ctcctcgatt ttttagctct gtcatgactg
     2881 acagccagaa tttggcacct tctgtctgag caatccacat acccagtaat tctttttgcc
     2941 catccatatt gatgcctaaa gcaaggtata cggacttgtt aatcacattg gagtgctgac
     3001 ggactttgac aacaatacag tcaagataga caacaggata aaggctatct aaggctctat
     3061 tttgccactc agtcacttgc tcaatcacag catcggtaac tttgctgatg agagatgctg
     3121 acacatcggc atcgtacatt tctttgaaga aggctacaat ttccctatta gtcattcctt
     3181 ttgcatacag tgagaggatt tggtcatcca tactggtgat gcgtgtttgg tgctttttga
     3241 taatttgtgg ctcaaatgaa ccttctcgat cacggggaat atctaaagcc agttgtccat
     3301 cttgagttgt aatggtttta gaactaaacc cattacggct atttgagcct ttcttgggct
     3361 gatgcttttc ataaccgaga tggtctgaaa gttcagtatt gagtgcagtt tcaatcatga
     3421 attttttaaa gactgctgtc atttggttta agtcttctgg tgtttttaga cctttagcca
     3481 attcggcagc catacttttg attgttgctt catccatgtg aagtaccttt tgtaattatc
     3541 ctctgaagga taaatgaaaa ttaagtactt acacaaaatt tagaacagtc cctcaaaaat
     3601 ttataaaagt aatcgtaata atgacgctct tcaaagtgac ttaattattg gagaattcta
     3661 tggagattca gacttattgt tagttcgatg tgatcctaat agtgatgatt acggctctat
     3721 ttttgttgtc ttacctatag atcaaagaca agattggtac attgttgcta atacttttca
     3781 agaattcatt agtaaatttt atgaaactca gggtgataaa ttctgggaac cttaaaatta
     3841 tatggccggt ataagctcta tttaacttat gttcacctaa aatttattta attatcacaa
     3901 aaatagtatc attttttgaa tttcatataa cagccattat gttaaataga atcaaaaaac
     3961 ctccaataaa atcgttatag tattattgtt tattgagtag ataagagaat taaattagtg
     4021 aaatactcaa atgaaaaaat agtaaaagct ctcttacttt ctcctttacc actacttttt
     4081 ttcacagcag tcctgtttat cgtaatgaat caggaataca gcctatactc gattttagtg
     4141 gttcttgtag ggcacggtct tgtctacttg gcttattgta ttttgactgt acctttcagc
     4201 ttcatattct caattctttt aaatcgttat aacagtctta atttattaac tatctgtatt
     4261 gcctctataa ttattgctac tccatttttt attctttttg gatggagtca cacaggagca
     4321 atttcgaaag agtggtggaa gatgtataca gatacatgga ctatctttat ggctctattt
     4381 cctggcttat gttattggct atttctgatt aacctaaaag ataagaaatc aaagaatatt
     4441 gaataaacca ttgcatagag attcttaaat ttccctaaaa ttaacataat acaccttata
     4501 cgaagcccag gttaatcctg ggctttttgt tgaatctgat cattggtagc aaacagatca
     4561 ggattggtaa ttttgatgtt ttcctgacaa ctcctgcaaa gcatcagccc agcaaaaagt
     4621 tgtacatgtt ccgttgattc acagaaggca catggcttag gaaaagagat gatattggct
     4681 gaattgacta attctgttga cataagaagt aaccttggat tgtacatatt tatttttaat
     4741 aaatttcaat actttatacc taatttagcc gctaaaattt gtacattatt gtataatcca
     4801 tgtgtacata ttatttttta taattttcat tcacttagac ccaaaatagt atttattttt
     4861 gtacaacacc atgtacagaa caataatcat ataaatcaaa tttttagcat aaaaaagtcc
     4921 attaattttg tacacaattc tgaaacttaa aaatctaaac tttcatcaat ttttttcata
     4981 atttcaataa attaacctta attttaagat atattctgaa agttggtttg aaagttgttt
     5041 ttacattata tttcaatact ttaaatcaaa aaattggata ttttttgaaa aactcaataa
     5101 aagtttattt tttatttaaa aacaactagt tatattagtt tttatccatt tttttgaaac
     5161 agtttctata tgataaaaaa acctataaaa accatatcta gcaaaggttt gagggttatc
     5221 atctttagat gcgtggtgtg tgacaaaaaa atcccggcat gtgccggatc cttgattaga
     5281 aaattggcta aagtgacgta ggactggtgc ttggttttat atagaaaaaa gtatttattt
     5341 cttggtttag aaaaacgtaa aaaaatcatt ttttgtccgt tcatccaaat taaaaatttc
     5401 aaactaaaat tttaattatg gaaattttca cagaaagcat tcaaatgcga tttaagagcc
     5461 tttaagtaaa aaacataggt cttatagtga aaaacagaaa acagctcaaa aaacgcaaat
     5521 gagcgcgaaa taaagaggga ttttgattct agatagattc atagagcgag tgtctacgag
     5581 cgaacttata aaatttgcct ctcagccttt ctgaaaaaca gtttttgggg ctttttagcc
     5641 taagaaagcg taagttttat gcagaaattt gttcctagac cgagcgtagc gagtaaaaaa
     5701 gttttatgag cgaagcgaat tccgagttgt ttttgctttt tcttaaaatc acgtaagtat
     5761 taaccaaaaa attgccccga cgaactgagc gaaagcgaag ttcaatagag tttgagcgaa
     5821 gcgaaaacca agggcaattt ttctttacct aggcttttaa ttatttttaa tcttttaaat
     5881 gcttttaagt aagctgtaac cctttattca taatagtttt agagttaata aacctaggtt
     5941 tatcgaccca taaaactagg tttatcgacc cataaaacta ggtttatcga cccataaaac
     6001 taggtttatc gaccttaaaa ctaggtttat cgaccccaaa ttaagtcgat ttacaattca
     6061 attaaaatag tctattgtac aacctagttt aattataaat ttattattag aaatgagtga
     6121 attaatcgta aaggataatg ctttaattca agctagctat accctagata cagttgaaca
     6181 aaggctgatc ctattagcca ttgctgaagc tcgggaaaca gggcacggga taactgaaaa
     6241 tagcctttta gaagtacatg caagtagtta tatcaatacc tttaatgtcg agaaacacac
     6301 tgcttatacc gtgcttaggg atgcatctaa aagcttgttt gatcgctatg tcacgtatca
     6361 tgacattaat cctaagactg gtaaggatcg tagctttcac tgtcgttggg tcgataaaat
     6421 tggatatgaa cctcaatccg ggatcgtttt cctacgtttc acgcaagaca ttgttccact
     6481 cataactcga ctggaagaaa actttactaa atatgaattg gagcaggttt cgagattaac
     6541 tagctcttat gctattcggt tatacgagtt attaattcaa tggagatctg ctggtaaaac
     6601 gccaattttt gatctatcga tctttagaca acaacttggt gtcgaagctc atcaatacaa
     6661 aacaatgagt aattttaaaa cgtatgtttt agattttgcc ctcaagcaag taaatgagtt
     6721 aaccgatgta aaggctaaat atgaacaaca taaaaaagga cgttcaattt ccggtttttc
     6781 tttcagtttt aagcagaaaa aaaccaactc tgacaaagta attaagggaa cggatacatt
     6841 agcccttttc accaaaatga gtgataaaca acgtcatctc tttgccaaca aactttctga
     6901 attacctgag atggggcaat attcagaagg caccgaaagt ttccaacaat ttgctatacg
     6961 cattgcagaa atgctccaag attatcaaaa attccaagaa ttatttcctt atcttcaaaa
     7021 agttggctat caagctgcct aaaatttatg cttgatcatt tgtctattag gcagtctaat
     7081 agacaacaac ttttgacagg tttagacata gatttacaac atgaaaagcc ttacagttct
     7141 tgagctttct aagctttaca atatcaatag acaaacgatt tacaacaaca taaagaaagg
     7201 aattttaagt aaaaattctg ataataaaat tgatctagcc gaggctatcc gtgtttttgg
     7261 tgaaccttca aaaaaacagg atgtaaaaga atctgtaaaa atagacagtc caatatcgac
     7321 agaagtttta ctacttagac aacagataaa catgctgcaa aatcagcttg atgatgcaaa
     7381 agagcgtgaa tccttttatc aaaatcaaat tgagacaatg caacgtctac tggaagcacc
     7441 taaacctgag atccaagagc ctagtgaacc cgaactccct caatcagctc ccattgtaga
     7501 aatgaaggag caggtgatag ttgttccacc taaagatgac ggattgacta ctccagagaa
     7561 taagcgcatt cctgttccag agcatgttga accagagcct aaaaagcgtg gcttctggag
     7621 tcgttttttt cttccatatg gttaacttag gctaaataaa accgtggaac attgctcaat
     7681 aaataagcgt ggaacgtgta aaatcataaa aaaaacccac cttggggaag atgggctata
     7741 aactagaaac tacagcattg atttctatag ataattataa cgcatttcgt ataaggtgta
     7801 ttatgttaat aatataaatt tttaaaagca tattgattta ataatagaaa aattatcaga
     7861 gtagggcatg tgtaaagtaa acgttcaatt attaaccaat aatgattaaa gatttatatc
     7921 ttgtatgtgt taattttatt aacatatata tatgaaatta ataatgcatt aagtgaaata
     7981 gtatgctagg tgaaaatcat cattttttaa aaaaattttc gatcagtgat gctgctatag
     8041 cgtataaaaa acctcgctcg actatactta aatatataaa aagcggagat ttcgaacttg
     8101 actacaataa attgattgaa tacagtcagt tagttagaaa atttggtgaa cttacttacc
     8161 ctatacagga cgaaagagag caacaaatca aaaaactaaa agatgaagtt atgcatttaa
     8221 aaaaacaact taactttttt aaatatactt caatctcaaa caaaaataga ctattaattt
     8281 ctcataaaca ttccatttaa cataatggtg cttatgcgaa atggcccttt aagtttactt
     8341 taagatcaat aacttacatt gagttaaacc cctggaacga tctcgttcaa ccgatttaga
     8401 aataaatcgg tttttttatg ttcaattttg atacttcttg ccaataaaat tgtttaaaaa
     8461 ataatcaaaa atagcaattc catagagttt ataagctttt ttacccaaaa gaatccacta
     8521 tttctgtgga taaccactgg gataaatttt gattattttt tgtacgtttt ggcttacagc
     8581 gtttcacgaa attgtctgta tctatccccc gatgaagctc ttatgatcga tctatatgga
     8641 caggatgttc aatgacttaa aacaacaata ataatcagtc aggcaccatg accatcaggt
     8701 agtcagccct acctatgcgc tccataccga aaaatcccga catgacgtcg ggatttttta
     8761 ttatctaaac ttcttaaatt aaagttggat gagcatctaa aatacgaatt aaagtcgctg
     8821 caggaccagt cggatagcga cggccttgtt cccagttttg cagggttctc gcgctgatat
     8881 gcaaacgtgc agcaaattca gcctgggtta agcctgtttt ctcacgtact tctttaatat
     8941 ccggtaattc aagctctgtg actcgacttg cctttatctc cttacgcttg atggcaccag
     9001 cttctttgat tgaagcaacc aggtcatcaa ataagttgtt atccatgaaa ttgctccttt
     9061 actaattgac gtagaatcga ggtttctttg tctgtcagat tatctttcac agactttgga
     9121 taagccacca ggaaaaagat ctgatcatct tctgtaaccc aataatagat cacacggata
     9181 ccgccacttt tgcctttatt gccctgagca caacgtactt tacgaattcc accaccattc
     9241 ttgattaagt cgcccttatc aggctgtacc aagagatctt gctggagctg acgatactct
     9301 tcatcactca caagctcttt gatttgctta gtaaaaatac tggtttcaat gaataacata
     9361 ggtttaagta cgtcaatggc gtataagaat tttagcagtt ttagaataaa aaaacgagag
     9421 ctgtgaactc tcgtttcgca taagcaaagg cttacaaata taaatattag gtataaaaaa
     9481 cccgcatgat agcgagtagg tcctgatatt tcattcagac tatagatttt ataaaaacta
     9541 tatttctaga tgattttaaa tatttttcca cttaaggtac tttgaaaaag catttcaaaa
     9601 ctaggtcgaa tatcttcacc atcaacggtg atatgtttta tattcaaatg agattgtgtg
     9661 ttggcaaact catgatccac tgtggcttga atagcgtgtt taacgccaaa ctggtctttt
     9721 acgctgagag tctgtttaaa catatatatt tccttaaaaa aattgcagtg gaaatcccac
     9781 ataaattatt gatgtttaaa aatttatcgc taagcaactt tatgatttaa tttaaatagc
     9841 tgatttagag aagtgtaaaa tgtacggcca tccaaattta aatcaacttc gattccagat
     9901 tccagtagaa ctcttttacc tacttcgatt tgttttaaac cttgacgtgt attttgcatt
     9961 ttatgtagag gaattaatga aacaataatt tcagtaccat ttttagattt tgcaattaaa
    10021 tcatttactt ttaacaactt atatcctatt ttttagagat tttttcaatt ttgaaaacat
    10081 tttaaagctt aaaaaaagca cttatgagtg ctttttatta ctgcttatta ttacttagat
    10141 agcaacaata tttacagcat tcggtccttt ttgaccttga gctacactga attcaacttg
    10201 ctggccttca aataaggtct tgaaacctga actcgcgatt tcgctgaaat gagcaaaaac
    10261 gtctggacct gattcttgtt gaatgaaacc aaaaccttta gtttcgttaa accacttaac
    10321 agtaccttta acaacatttg agtttgacat aatatatcct actaattttt aataattttt
    10381 tagtcatttt attgactgat taataacttt gaaaataata aagaatgaga cttaaaatct
    10441 aaaaaacgaa ggattatgac taaaactgcg atacttaagg aagatttacc gaaacaagac
    10501 tttttttcta gttaagttaa atatacagta aaaataaaaa taatcaagtt ttttgatata
    10561 aatattaatt tttcttataa aatccatatt tttttcttta aaaattaatt aaattcaata
    10621 agttatttat tatattattt aatattttct ttaacataaa atctcttatg cgaatctaga
    10681 aggattaagc tatttcgtaa tattttaacc aataagcatt gtcaaaaata tcaatgtaat
    10741 agcttggcct cttacaatgt gaaaaatatt cacagcataa atctctaata atttcaattt
    10801 cttctaggct atcggcagca ttgactaaaa atataaactt gtctaagtta acaagtaatt
    10861 tagtactatt tttcaaaggt ttattattag acattgaacc ctatgtgttt tcctgttgga
    10921 agctctacat caatacgaag ttttccaccc attgcttcaa catacttttt catcgttgaa
    10981 attttaagat catgaccacg attttcgatt gctgataatg acggctgttt aattccaagg
    11041 gttgcagcta gttctttctg ggaaatttct aactcttctc ggataaggtg aagctgactt
    11101 tctaaaagta attcatcagc cattttttga atacgagctt gactctctgg agagcgacta
    11161 gccaataatt cttgtaagct cttagccatt agattgatct cctaaagttg agagatgaag
    11221 tgcgtattgc tcatctgcaa ttgctagcat ctctgtataa aaacgctttt tattactctt
    11281 atcaccaata cacagcacaa tcgct
//
