LOCUS       NZ_CP025947             2443 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Escherichia coli strain SCEC020023 plasmid p3_020023, complete
            sequence.
ACCESSION   NZ_CP025947
VERSION     NZ_CP025947.3
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07768275
            Assembly: GCF_002850675.4
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain SCEC020023
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 2443)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   4  (bases 1 to 2443)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002850675.4-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 03:24:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,022
            CDSs (total)                      :: 4,900
            Genes (coding)                    :: 4,638
            CDSs (with protein)               :: 4,638
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 82 of 262
            Pseudo Genes (incomplete)         :: 195 of 262
            Pseudo Genes (internal stop)      :: 44 of 262
            Pseudo Genes (multiple problems)  :: 54 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP025947.4.
            On Aug 26, 2019 this sequence version replaced NZ_CP025947.2.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCEC020023"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p3_020023"
                     /geo_loc_name="China: Sichuan, Yibin"
                     /collection_date="2016-10-27"
     gene            complement(join(1929..2443,1..454))
                     /gene="mobV"
                     /locus_tag="C1A20_RS00640"
                     /old_locus_tag="C1A20_00305"
     CDS             complement(join(1929..2443,1..454))
                     /gene="mobV"
                     /locus_tag="C1A20_RS00640"
                     /old_locus_tag="C1A20_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            1433..1867
                     /locus_tag="C1A20_RS00645"
                     /old_locus_tag="C1A20_00310"
     CDS             1433..1867
                     /locus_tag="C1A20_RS00645"
                     /old_locus_tag="C1A20_00310"
                     /inference="COORDINATES: protein motif:HMM:NF017542.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004193480.1"
                     /translation="MSNRITQIDTATGEEVGSFVAVIRPKQKSAFERHFTMNQDALKIL
                     AKTLTGEQFKVLMMLLADLDYENYIQIAQADIADALGMQKTNVSRSVKALIDLSVILQG
                     PKVGRSYSYRLNPQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(3..463)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.156"
                     /coverage="96.0"
                     /evalue="2.45e-97"
     Typing          complement(1933..2441)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.882"
                     /coverage="96.0"
                     /evalue="8.140000000000001e-86"
ORIGIN
        1 gcatctttga ccgcccgcca ataaactctt tcgcggacag ccgtccatcc tgcgtcaatg
       61 gcaccacgaa cgcagacagg tgcggggtgg cctcgtcccg gtgaaccacc gccgccacaa
      121 cacgatcctt cccgtatttc ttctccagcc attgctcaga gcgggcgaaa aactccgcct
      181 gctgccgtgg tgtggcctct ttccaccact cagggcttgc tgtcataacg tattcaacgg
      241 ccagtaccgc atctttacgg cgtttttccg gcagcagctc ccgcacacgc cccattgcct
      301 gatccgttga tttggacaca gagcagtagt tttctggtgt ccgttcagcg ttagcgttcg
      361 gcgtctcacg ctctcggtag cagtgctgca acgcgctggc cacgcttccc attcccgtca
      421 gctttttgca gcgcattatc gcgtagttcg ccatgttccc ccctgattcc ggcattcatt
      481 cagccaggcg ggggagcgca ttgcgatacc tccggcgggc cactatcaag atgatagtgt
      541 actcactaga ctttgctacg caaaatcgtt cgggcagcaa gctgccagaa ggtcaaaatc
      601 aacggcggtc tggcaggtgg aaggcgtcgc ttcgcgccac cccccaccac tgccaagcct
      661 gttttatccg gagtgtcgtc aagggtaaat ggcactcgct acgctcgccc ttgacgaaat
      721 caggcaatga aatcgtcttt gaaagagcag gaaccgctcg ccgcagcttt ggctgaggcg
      781 taccgcagga gcaacgcgac gaggaacgaa cagccaaagc gaggaagcgg aatttccact
      841 ccccgtacat agaaatgcgt acactccttg ctaaaaaaag gcagtttttc agtgtctaaa
      901 aatgccgttt ttcagccctt attttccccc ttaatatata gatatagata tgggaacccg
      961 cttgtaccaa gggctagagc gaagaatcgt actcacctca agtaccaatc gtactcacct
     1021 caagtactaa tcgtactcac ctcaagtact aatcgtactc acctcaagta ctaatcgtac
     1081 tttaggtatg gcactcgcta cgctcgccct tgacgaatgt caggcaacaa agtcgtcttt
     1141 gaaagagcac gcgccgctcg ccgcaggtca ggctgaggcg taccgcagcg cagcgaggaa
     1201 cgaacagcct gaacgaggaa gcggaatatc acctgaacgg gcatagaaat gcttacgcac
     1261 gaaccgatga aaatagggtt cgctatccgg gcatatgagc ttctcgctat aagatacaga
     1321 agagagcctt ctctcagccc ttgtggggcg cggctctcgc ttgacgatag ttatcattat
     1381 tgataacgtt gttatcatta ttgataacta tttttttggg agagcctctt aaatgagtaa
     1441 ccgaattact cagatagaca ctgctacagg ggaagaggta ggtagttttg tcgcagttat
     1501 ccgacccaaa cagaagtcag cttttgagag gcatttcact atgaaccaag acgctttaaa
     1561 gatccttgct aagaccctca caggggagca attcaaagtc ttgatgatgc tgttagctga
     1621 tctcgattat gaaaattaca tccaaattgc ccaagccgat attgccgacg ctttaggtat
     1681 gcagaaaacc aatgtaagcc gttcggttaa ggcgctaatt gacctatccg tgattcttca
     1741 aggcccaaaa gtagggcgca gttacagtta ccgtctgaac cctcagtttg gctggaaagg
     1801 tactgtcagc aaccacaaga aagcactaaa aaacggcctc agcgttatac agggcggcag
     1861 aacctaatct ggaatgccgc aaatgacgtc actttgcggc gttagcccta tcctgcatcg
     1921 catcaaaact aacgctccat accacccatg ccacgcgatc gcgtctgtcg ctgtttttca
     1981 cgttcaatac gctgctgttt ttccttttcc tggttttgct gcttaaaccg ctgagccatc
     2041 atcaggattt cggtcatctg ctctctggaa agccccttaa aaggctctgt gacgctctgt
     2101 aggcgtttct gctgcgcatc catggtcttc tgtagctcac gcgctctttt tgcgttctgg
     2161 gcgctctgag aggccatagc gatggttcct gcaaagccat cgttcactgc gtggcttaga
     2221 cgtttggcta tggctgcctg atcttccacg tctttggtga aaatgccctt ccgcaataca
     2281 cgcggctcca gcgcctccgg cgagatcgat acctggctac gggtgccacg gtttatcgat
     2341 tcgtagtagt gctgaactgt ctgatgcgtg gcgcggcttc cctcgatgcc gcgctcaaga
     2401 cccagttttt ttactgattc agcgtaggtg ctctgatctt cac
//
