LOCUS       NZ_CP025946            12819 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Escherichia coli strain SCEC020023 plasmid p2_020023, complete
            sequence.
ACCESSION   NZ_CP025946
VERSION     NZ_CP025946.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07768275
            Assembly: GCF_002850675.4
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12819)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain SCEC020023
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12819)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 12819)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002850675.4-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 03:24:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,022
            CDSs (total)                      :: 4,900
            Genes (coding)                    :: 4,638
            CDSs (with protein)               :: 4,638
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 82 of 262
            Pseudo Genes (incomplete)         :: 195 of 262
            Pseudo Genes (internal stop)      :: 44 of 262
            Pseudo Genes (multiple problems)  :: 54 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP025946.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCEC020023"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2_020023"
                     /geo_loc_name="China: Sichuan, Yibin"
                     /collection_date="2016-10-27"
     gene            1..951
                     /locus_tag="C1A20_RS00600"
                     /old_locus_tag="C1A20_00265"
     CDS             1..951
                     /locus_tag="C1A20_RS00600"
                     /old_locus_tag="C1A20_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946646.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001293213.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
                     /name="replication initiation protein"
     gene            959..1372
                     /locus_tag="C1A20_RS00605"
                     /old_locus_tag="C1A20_00270"
     CDS             959..1372
                     /locus_tag="C1A20_RS00605"
                     /old_locus_tag="C1A20_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001229901.1"
                     /translation="MALVSISEASRLTNKSRTTVHRYISKGKLSICTDENGVKKIDTSE
                     LLRVFGAFKAVHHEQIDNVTDEQHVTPNRTGETSKTKQLEHEIEHLRQLVTAQQSHIDS
                     LKQAMLLIESKLPTTREPVTPPVGKKSWQFWKK"
                     /name="hypothetical protein"
     gene            complement(1607..2479)
                     /locus_tag="C1A20_RS00610"
                     /old_locus_tag="C1A20_00275"
     CDS             complement(1607..2479)
                     /locus_tag="C1A20_RS00610"
                     /old_locus_tag="C1A20_00275"
                     /inference="COORDINATES: protein motif:HMM:NF037202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="WP_172619769.1"
                     /translation="MMHRVSKIPEIFPEKNSNNLIIIITGRGSTKGFSSLISNEIVDLE
                     CISKGQCFPLYVYDNKKPSILFGNQDESVYLDGEYERKYAISDTGMIYFKKHYPEEKIN
                     QEDVFYYIYGLLHSEDYRSRYADNLSKELPYIPCVKKIEDFWSFSKAGRELAELHLNYE
                     TVEPYKADIDTGSLSYSQLGKDDFYVEKMKFAKKGEKNTVIYNNKIRIKNIPIEAYDYI
                     VNGKPALEWVMERQGVSTHKDSGIVNDANDWAIETMDNPRYPLELFLRVITVSLETQKI
                     VNSMPKLDI"
                     /name="type ISP restriction/modification enzyme"
     gene            complement(2556..5573)
                     /locus_tag="C1A20_RS00615"
                     /old_locus_tag="C1A20_00280"
     CDS             complement(2556..5573)
                     /locus_tag="C1A20_RS00615"
                     /old_locus_tag="C1A20_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490160.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element IS3000 family transposase"
                     /protein_id="WP_004199413.1"
                     /translation="MAFEERVQILSEAEQDELYGPPAFTSADQRFFFSLNDKELAIAKS
                     LRHRGQRYMLVVLLGYFKAKPVVLNPGFHQIKQDLKYVYQTVLPGPGCRPFNLTPKENE
                     RIYQRVFQLCNYQRWNVKDHGAALRDYLSQQARAWTAPRHLFDAANEYCSGQKIAIPAY
                     STLQKIISQVVGDEQEHMAAHLERAMSRGLKQALAELVNGTGPLPFRQLRQSARNFTGT
                     ELEKELIVYRHIQHWMPEVDLLLSTLSLSQKNLQHLAEKVDYYGAKLKRQTVGSQWLYL
                     LCYLQTRWQQALERIADGFVHHVRQTKQKAKDYAQEAVFKDWQKAAKNVSKAAEVLHLF
                     IDDSIDLQLPFATVRQQALSLLTKRDLESVCLFLNEQRRSVDEAMWQYCDEKESLRKGL
                     LRELFLCLRFEGCDGTQHLAAALAKTQNELNGQDAQLQTADTRLLSKKSREFLLDGEGN
                     ILIDRYEWFLYQQIPDRLNGQLTLPDITKYRALDADLIDGEHWRKNKYTLLQQSHFTKL
                     AEEPEKLIKQMAMELDTRLYEVGEYLEQEDNRNIILRNPQGKRFWRLPSASKHHLVNNP
                     FFQQIPTTGIADVLRMVDRDTGFIDCFAHVLGSQSRSRSHEYDLLAILVGNATNQGIYG
                     MAQISDRTYDQLSTIQANYLRLETLNAANDNINNATAKLPIFRYYNIQEDVIHASADGQ
                     KFEARRETFKTRYSSKYFGTQKGVSAMTLIANHAAINARVIGANEHESHYIFDLLMSNT
                     SDIIPDVLSTDTHGVNHVNFALLDLFGYQFAPRYAQVGKVINDMFDVKEDKEHRIQLCL
                     KKPINTHRIAQHWDTIQRIAVSLKQRKTTQATLVRKLSEYKRNHPLLEALTEYNRLVKA
                     NYLLCYIDDASLRNYVQRALNRGEAYHQLRRAVSSVNGDQFRGSSDEEIQLWNECARLV
                     TNAIVYFNSRILSQLLTSFEYQGDTKRIDIVKQASPVAWHNINLKGTYHFELSEKLPDL
                     EELMRSIEGYLPVSEK"
                     /name="Tn3-like element IS3000 family transposase"
     gene            complement(<5727..9770)
                     /locus_tag="C1A20_RS00620"
                     /old_locus_tag="C1A20_00285"
                     /pseudo=""
     CDS             complement(<5727..9770)
                     /locus_tag="C1A20_RS00620"
                     /old_locus_tag="C1A20_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005204747.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:0016787 -
                     hydrolase activity [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /name="DEAD/DEAH box helicase family protein"
     gene            complement(9883..10467)
                     /locus_tag="C1A20_RS00625"
                     /old_locus_tag="C1A20_00290"
     CDS             complement(9883..10467)
                     /locus_tag="C1A20_RS00625"
                     /old_locus_tag="C1A20_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_101975723.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQAASSQLQQQSETLSR
                     VIRELDSASGNMTDTVRKSVSSALTQVEKELKQAGLAQQKPATEALNQAADTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVLASCVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTHTTYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            complement(10480..11595)
                     /gene="mobA"
                     /locus_tag="C1A20_RS00630"
                     /old_locus_tag="C1A20_00295"
     CDS             complement(10480..11595)
                     /gene="mobA"
                     /locus_tag="C1A20_RS00630"
                     /old_locus_tag="C1A20_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_101975722.1"
                     /translation="MASYHLSVKTGGKGSASPHADYIAREGKYAREKDSDLEHKESGNM
                     PAWAAHKPSEFWKAADTSERANGCTYREIEIALPRELKPEQRLELVRDFVQQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERINDGIHRDPEQYFKRANTKEPERGGAKKARFG
                     ETPTERKEYLVAQRERWADLQNKHLERYQHADRVDSRSLKAQGIDREPERHLGAGQVQR
                     FDTEQLQAILERREAERQVQQSRDERDSVIDVTTSLREALSERDTLTLKQELKSEPEQE
                     SHSGRTFDFEKEPDKLNALVSDAMKDIQEEIDLQSLVNDAMAEFQGIHQEMERQRERER
                     LVEKQRQQEKERQRLAEQIRQKPDKGWSFSR"
                     /name="mobA"
     gene            11759..12226
                     /gene="traD"
                     /locus_tag="C1A20_RS00635"
                     /old_locus_tag="C1A20_00300"
     CDS             11759..12226
                     /gene="traD"
                     /locus_tag="C1A20_RS00635"
                     /old_locus_tag="C1A20_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_016946647.1"
                     /translation="MNKSEKQEQWAAERVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSKTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSTT"
                     /name="traD"
     Typing          776..951
                     /name="ColE10"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          complement(10484..11595)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.801"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtctgaat tagttgtttt caaagcaaat gaactagcga ttagtcgcta tgacttaacg
       61 gagcatgaaa ccaagctaat tttatgctgt gtggcactac tcaaccccac gattgaaaac
      121 cctacaagga aagaacggac ggtatcgttc acttataacc aatacgctca gatgatgaac
      181 atcagtaggg aaaatgctta tggtgtatta gctaaagcaa ccagagagct gatgacgaga
      241 actgtggaaa tcaggaatcc tttggttaaa ggctttgaga ttttccagtg gacaaactat
      301 gccaagttct caagcgaaaa attagaatta gtttttagtg aagagatatt gccttatctt
      361 ttccagttaa aaaaattcat aaaatataat ctggaacatg ttaagtcttt tgaaaacaaa
      421 tactctatga ggatttatga gtggttatta aaagaactaa cacaaaagaa aactcacaag
      481 gcaaatatag agattagcct tgatgaattt aagttcatgt taatgcttga aaataactac
      541 catgagttta aaaggcttaa ccaatgggtt ttgaaaccaa taagtaaaga tttaaacact
      601 tacagcaata tgaaattggt ggttgataag cgaggccgcc cgactgatac gttgattttc
      661 caagttgaac tagatagaca aatggatctc gtaaccgaac ttgagaacaa ccagataaaa
      721 atgaatggtg acaaaatacc aacaaccatt acatcagatt cctacctaca taacggacta
      781 agaaaaacac tacacgatgc tttaactgca aaaattcagc tcaccagttt tgaggcaaaa
      841 tttttgagtg acatgcaaag taagtatgat ctcaatggtt cgttctcatg gctcacgcaa
      901 aaacaacgaa ccacactaga gaacatactg gctaaatacg gaaggatctg aggttcttat
      961 ggctcttgta tctatcagtg aagcatcaag actaacaaac aaaagtagaa caactgttca
     1021 ccgttacata tcaaagggaa aactgtccat atgcacagat gaaaacggtg taaaaaagat
     1081 agatacatca gagcttttac gagtttttgg tgcattcaaa gctgttcacc atgaacagat
     1141 cgacaatgta acagatgaac agcatgtaac acctaataga acaggtgaaa ccagtaaaac
     1201 aaagcaacta gaacatgaaa ttgaacacct gagacaactt gttacagctc aacagtcaca
     1261 catagacagc ctgaaacagg cgatgctgct tatcgaatca aagctgccga caacacggga
     1321 gccagtgacg cctcccgtgg ggaaaaaatc atggcaattc tggaagaaat agcgctttca
     1381 gccggcaaac cggctgaagc cggatctgcg attctgataa caaactagca acaccagaac
     1441 agcccgtttg cgggcagcaa aacccgtact tttggacgtt ccggcggttt tttgtggcga
     1501 gtggtgttcg ggcggtgcgc gcaagatcca ttatgttaaa aacgacagga tttatgaaaa
     1561 taaccagaat tgtgcaggat gttttttcat cctgcacacc tagtgattag atatctagtt
     1621 ttggcatgct attaacgatt ttctgagttt caagactgac tgtgataact ctcaggaaaa
     1681 gctctagtgg gtatctaggg ttatccatag tttcaattgc ccagtcatta gcatcgttca
     1741 caatcccgct atctttatgg gtagaaactc cctgccgctc catcacccac tcgagagcag
     1801 gcttgccatt cacaatgtag tcataggctt caatagggat gttttttatg cgaattttat
     1861 tgttataaat aacggtattt ttttcacctt ttttggcgaa cttcatcttc tcaacataaa
     1921 aatcgtcttt acctaactga gaataactca gggatcccgt atcaatatca gccttgtatg
     1981 gctcaactgt ttcgtagttt aaatgcaact cagcaagttc acggcctgct ttagaaaacg
     2041 accaaaaatc ctcaattttt ttcacacatg gtatatatgg caactcctta ctcaaattat
     2101 ccgcatagcg agagcgataa tcttcactgt gcaacaaacc ataaatgtaa taaaaaacat
     2161 cctcctgatt tattttttct tccggataat gctttttaaa ataaatcatc cctgtatcac
     2221 taatggcata ttttcgctca tactcaccat ctaaatacac actttcatct tgattgccaa
     2281 acaaaatact aggtttttta ttatcgtaaa catagagagg gaagcattgc cccttagata
     2341 tacactcaag atcaacaatt tcatttgata ttaatgaaga gaacccttta gttgagccac
     2401 gccctgtaat aatgataatt aaattattag agtttttctc tgggaaaatt tcaggaattt
     2461 tgctaacacg atgcatcatg gggttctagg gattttccgt ccaaaaacga caaagtgctc
     2521 tggaggccgc gccgtccgtg gcctccagag gggtattact tttcgctgac gggtaaatat
     2581 ccctcgattg agcgcataag ctcctccaga tctggcaatt tttcgctcaa ttcgaagtgg
     2641 taagtcccct tgaggttaat gttgtgccag gccacagggg atgcctgttt gacgatatct
     2701 attctcttgg tatctccttg gtattcgaag ctggtcaaca gctggctgag tatcctggag
     2761 ttgaagtaaa cgatggcatt ggtgaccagg cgagcgcact cattccatag ctggatttct
     2821 tcgtctgaac tgccccggaa ctgatcccca ttgacgctgc tcacggcccg acgcagttgg
     2881 tgataggcct ctccccggtt cagcgcgcgc tgaacatagt ttcttaaact ggcatcatcg
     2941 atgtagcaca gtagataatt cgctttcacc aggcgattgt attccgtcag ggcttccagc
     3001 agcgggtgat tgcgcttgta ctccgagagc tttctcacca aggtggcttg cgttgttttc
     3061 cgctgcttaa gtgatactgc aatccgttgg atggtatccc agtgctgcgc aatacgatgg
     3121 gtattgattg gcttttttaa gcacagctga attcggtgtt ctttgtcttc cttgacatca
     3181 aacatgtcat tgatcacttt gccaacctgg gcatagcgtg gggcaaactg gtatccgaac
     3241 agatccagta acgcgaagtt cacatggttc accccatggg tatcggttga gagcacatcc
     3301 ggaatgatgt ctgacgtatt gctcatcaac aaatcaaaga tgtagtgcga ttcatgttcg
     3361 ttggcgccga tcactctggc gttgatcgca gcgtgattgg cgatcaaggt catggcagaa
     3421 acaccttttt gagtgccaaa gtacttcgac gaataacggg ttttgaaggt ctcgcgccgg
     3481 gcttcgaact tttgaccatc ggcactggcg tggatcacat cttcctggat gttgtagtac
     3541 cggaagatgg gtagcttggc tgtcgcgtta ttgatgttgt cgttagcagc attcaatgtt
     3601 tccaggcgaa gatagttcgc ctggatagtg ctgagctgat cataggtacg atcagagatc
     3661 tgtgccatgc cgtaaatgcc ttgattggtt gcattgccga ccagaattgc caacaggtca
     3721 tattcatggg aacggcttct ggattgggaa cccagcacat gagcgaagca gtcaatgaaa
     3781 ccggtgtcac gatcaaccat gcgcagtaca tccgcaatcc ccgttgtggg aatttgctgg
     3841 aagaagggat tgttgaccag atgatgtttg ctggccgaag gcaggcgcca gaagcgttta
     3901 ccctgcggat tacgcaagat gatattccgg ttgtcttcct gttcaagata ttcgccgact
     3961 tcgtacaaac gggtatccaa ttccatggcc atctgtttga tcagcttttc aggctcctcc
     4021 gctaattttg taaaatggct ctgttgaagc agcgtatatt tgttttttcg ccaatgttcc
     4081 ccgtcgatca ggtcggcgtc gagtgcccgg tatttagtga tatcaggcag cgtcagctgg
     4141 ccattcaggc gatcaggaat ctgttgatag aggaaccact cataacgatc gatcaggata
     4201 ttcccttcac catccagcag gaattcacgt gattttttgg aaaggagtct ggtgtcggca
     4261 gtttgcaact gagcgtcctg gccgttgagt tcgttttgtg ttttcgccaa ggcggccgct
     4321 aagtgctggg tgccgtcgca gccttcgaag cgcagacata ggaacaactc tcgcagcaaa
     4381 cctttccgca gactttcttt ctcgtcgcag tactgccaca tggcttcatc gaccgatcgt
     4441 cgctgctcgt tcaggaagag acatacagat tccagatccc ttttggtcag caggctcagt
     4501 gcttgctgtc ttactgttgc aaacggtagt tgcagatcaa tgctgtcatc aatgaacagg
     4561 tgcagtactt cagctgcctt gctgacattt ttagctgctt tttgccaatc cttaaacacc
     4621 gcttcctgcg cataatcctt tgccttctgt ttggtctgtc tgacatgatg cacaaagcca
     4681 tcggcaatac gttccagcgc ttgctgccat cgcgtttgaa gatagcacaa taaatacagc
     4741 cattggctgc ctacggtttg acgtttcagt ttggcaccgt agtagtcgac tttctctgcc
     4801 aggtgttgca gatttttctg cgacagtgat agcgtgctca acagcagatc cacttccggc
     4861 atccaatgct ggatatggcg atagacaatc agttctttct ccaactcggt tccggtgaag
     4921 ttacgagccg attgtcgcaa ctgtcggaac ggcagtggac ctgtgccgtt cacgagctcc
     4981 gccagtgctt gcttaagacc gcgtgacatt gcacgctcaa ggtgcgcggc catgtgttcc
     5041 tgttcgtctc ccaccacctg actgatgatc ttttgcagcg tgctgtaggc agggattgcg
     5101 atcttctgcc ccgaacaata ttcattggcc gcatcgaaga ggtgcctagg cgctgtccag
     5161 gctctggctt gctgggataa gtaatctctc aacgccgctc catggtcttt gacattccag
     5221 cgctggtagt tgcagagctg gaaaacacgt tggtaaatcc gttcgttctc ttttggcgtg
     5281 agattaaagg gtctacagcc tgggcctggt agaacggttt gataaacgta tttgaggtcc
     5341 tgcttgatct gatgaaagcc gggattcagc accaccggct tggctttgaa atagcccagc
     5401 agtaccacca gcatgtaacg ctggccccga tgacggaggc ttttagcgat cgccagttcc
     5461 ttatcgttca gcgagaaaaa gaagcgttgg tcggcggagg tgaaagcggg gggtccatat
     5521 aactcatctt gttctgcctc ggacaagatt tggacacgtt cttcgaatgc catggattta
     5581 gcccaaaaag actaaatctt actcaaacag agcccaaata gggatctcga aaaaagtaat
     5641 acccctctgg aggccacaga cggcggggcc tccagagcac tttgtcgttt ttggacggaa
     5701 aatccctaga accccatcat gttctcatta aagtaaacca aagacttact atatggtcta
     5761 tataaggaaa ctctaacaac accatcagca atatcataat atctatctct ggagatttct
     5821 ttgtaacttt ccccatacca agcaaaatta aatgggtcaa ttcttacaaa gtccttcaag
     5881 ctccctgaat tatattcttt tttataccga tcaacttcag aattaaaaaa attaatggac
     5941 ttttttatgt tcatggctaa tttttgtttt gagtagttat aaaaccatgc gtcacctcta
     6001 ggctgaagcc ctgttgaata aacatcgaaa agagatgagt tttctttatc ttttttattg
     6061 cctatttgga taaagtcata aaaactgtca tcacgctgat tcagccaatc gttgtaggca
     6121 tctggtgtga ttttctgcca gccatgcgtt ttctcaatac cgtcaatact gccaaaatcg
     6181 gaaatcttct caagcttttc ctcacgagtc agatagtcac caatatcatg gaaataaatc
     6241 tgaccggact gtgttgcagc cggatttttt acaagaatag caatgacaat cggtgttctg
     6301 gttccctgcc caaaaacgtt atctttttcc ttgcggcgga cttctccaga cgttctcgca
     6361 tttcccctca agtggaaaaa atacaggcta ctgaactctt cagccagaca cttacgcaac
     6421 ccgtcagccg agttagagtc aatatagcca ccattagtca caaaaccgat tacaccttgc
     6481 tcaccgatac ggtcactcgc ccagcgaata gctcgaatat aggagtcata gagtgcattc
     6541 ttgttggtcg cagtggtaca agcggcatac gtctctgcaa tccgcttatc gagcatcgga
     6601 tattttacat tctggttatt atcgttcgcc gattcctgac cggatgagta aggcggattc
     6661 ccgataatca cccgaatatc cagagccttc tgccgtttac gacgttcact gttatctacc
     6721 agaacctcac tgaccagatc atccttctca tacatttcaa aggtatctgt caggcaaatc
     6781 ccttcaaatg gcgtgtactc tgattcttcc gtgatgctgt gatacaccgc ctcaatatta
     6841 atggcggcaa tgtaataggc taacaagacg atttcgttgg catggatttc atttttaaac
     6901 ttgtaagcca gtttatctga tgggataaga ccactttgca gcaggcgggt gataaacgta
     6961 ccggttccgg tgaatgggtc aagaatatgc acacctttgt cggcgagtgt ttgtccgaac
     7021 tcctttttca gcacgtcatt cacgctgtga ataataaaat caaccacctc aacaggcgta
     7081 tacacaattc ccagacgctc agtcattctc gggaacgcat ttctgaaaaa cttatcgtac
     7141 aactgtacga taatctgctg tttaccttct gcggtggtaa tgccgtctgc acgcattttt
     7201 accgactcat agaagctgtt cagcgtgctg gcttcttttt cgagatgatg ctcctgtaac
     7261 gtgttaagca cgttctgcat cgccatactc atcgggttat ggtcggtaaa attgtaatcg
     7321 gcaaacaggg catcaaagac cggcttggtt atcagatgct gtgccagcat ttcaatgatt
     7381 tcatcgtcgg aaacactgtt attcagatca tcacgcagtt catcggcaaa tgccctgaag
     7441 gcactgattt ctacttgatt acccggattt tcgagaatgc cgtgaatgcg gtcgatatgc
     7501 gtgcgggcaa ttttagcgat atcgttcgcc cagtcttccc agtgatgccg gttaccgcat
     7561 tttttaacga ttttcgcgta cagggcacgt tcaatctcac caacggaaaa ctcaatatca
     7621 aactgctcag gaacaggact cacggctgaa ccaatgccac tgcttttacg cgcttttcct
     7681 gccagttcag attttttcgt ctgccgttta gttttcttca ccactttatc ggctaccgcg
     7741 atcacttcca ttctgctggt atctttaccg ttaaactcca gcttgttgat catggcatca
     7801 aagcggtcat catgcgccct gagtgcattc agcacctgcc agacaacacg gtaggcctga
     7861 ttgttgttta aggcgttttc cggctcttct ccggcgggaa tgacaaccgg cagaatgaca
     7921 tagcccagct ctttaccttc ggcacgccgc ataacacgcc cgacagactg caccacatcc
     7981 acctgagagc ttctcggcgt taaaaacaac actgcatcca gagaaggcac atccacacct
     8041 tcagataaac aacggacgtt agacaatatc cggcagacat tttcatccgt atccgctttc
     8101 agccattcaa ttttggattt tttctcagtg gcattcatgc tgccgtcaac gtgcttcacc
     8161 tgacactgaa gttttaatga cggatcaata gccttatccg gtgcggtttc cctgagaagt
     8221 tcaatctcgg atgcctgata ggcttcaacc actgctccga acatttcagt gatcagttta
     8281 gaactgacct tatgcgtttt gccgcgaaag tctttttcta tcacctgaca gaacgccact
     8341 gcccgcttca tcgcatcagg atgaacgcca tcagaccccg ccagcccctg ctttgccagt
     8401 gctttccagc aacccacaat ttttgccgca tcatcgactt tgagctgatt attttcatcc
     8461 ttcagcagag actgaatgcg gcggctgaca tgcttttctt ccacggcaag cacaataact
     8521 ttgtaatcac acaaaatgcc caactgaacg gctttagaga aggtaatgac atacaggtca
     8581 tcaccgtaaa ggcttttgtc atccattgag cataaggtga ccccttcagt ctctttcgca
     8641 tcgtccccga aaatgcgcgg agtcgcggtc atgtacaggc gtttttgacc ggcaatatag
     8701 gcgttgtcgt gaatacgaac aaaggcggac tcatcatccc cttcaaaggt cgcacctgtg
     8761 gtccggtgag cttcatcaca aatgatgaaa tcaaatgcag gaaaacccag tttttgcgcc
     8821 tgatggatga catcaacaga gtggtaggta gaaaacacca cgttcatata aggcggctta
     8881 tcactgccga agatatcagg ctgattaaac gctttgacga ggctgttcgc attcgtggtc
     8941 gccggatatt tcaggtcgct gataccaaca acgacattgt catcatggtt tttcttaccc
     9001 acatcactgt cagagcagac agcaaaacag cgcaggtcta tcagcgtatc ctgtgtccat
     9061 tcatccagcg tctgactcaa caatgccaga ctgggcacaa ggaacagtac ggttttcccc
     9121 cgtccggcaa ccgcttcagc gatacgcagc gaggtgaagg ttttccctgt gccgcacgcc
     9181 ataataagct tgccgcggtc ggcagtcgtc agccctcttt tcacggcttc aagcgcggga
     9241 gtctgatgag gtcggagttg tttttttgct ttcatcaccg gtttgtaggc cggatcaaaa
     9301 tcgaattgtg accagtccag tgcgctttgc tcaaggtctg tcaggcttaa cgcagtgaca
     9361 ggaacggcct tgtcccgcag catattttta gcttcctccg tccagttatc cgtagaggca
     9421 acgatatagc ggagcgtaaa atacgtttta tcggaggcag ccagaaaact atcaatatcc
     9481 tttttggcga ttttagtctg attgtagttt ttgcactgga tagcatgaaa ctcccccgtt
     9541 ttggttacgg cgaccaaatc aatgccatca tcttttttct tcgtgatccc aacggtttca
     9601 ccgtactgag aaacccatcc gccatagctc agcacgtcac tgtacagctc ggcatattta
     9661 ggttcatgga ggaaatactg cacacaaagg ttttcaaagg ttgtaccttt gtcccgctgt
     9721 gttggtgaag cctgttcaat ttttgtcagc aatgcagaaa gtgcattcat cgttgatatc
     9781 cttaatgtcc tttatttgct ggggttatca gatccccccg acacgtttaa ttaatgcttt
     9841 ctccgccgga gatcgacgca cagcgttctg tgctctatga tgttatttct taataatcat
     9901 ccaggtattc tctttatcac catacgtagt gtgagtgtcc accttaacgc agggctttcc
     9961 gtcacagcgc gatatgtcag ccagcggggc tttcttttgc cagaccgctt ccatcctctg
    10021 catttcagca atctggctat acccgtcatt cataaaccac gtaaatgccg tcacgcagga
    10081 agccaggacg aagaatatcg tcagtacaag ataaatcgcg gatttccacg tatagcgtga
    10141 catctcacga cgcatttcat ggatcatcgc tttcgccgta tcggcagcct gattcagcgc
    10201 ttctgtcgcc ggtttctgct gtgctaatcc ggcttgtttc agttctttct caacctgagt
    10261 gagcgcggaa ctcaccgatt tcctgacggt gtcagtcata ttaccggacg cgctgtccag
    10321 ctcacgaatg accctgctca gcgtttcact ttgctgctgt aattgtgatg aggcggcctg
    10381 aaactgttct gtcagagaag taacacgctt ttccagcgcc tgatgatgcc cgataagggc
    10441 ggcaatttgt ttaatttcgt cgctcataca aaatcctgcc tatcgtgaga atgaccagcc
    10501 tttatccggc ttctgtcgta tctgttcggc gagtcgctgt cgttctttct cctgctgacg
    10561 ctgtttttcc accagacgtt cgcgctctct ctgcctttcc atctcctgat gtatcccctg
    10621 gaactccgcc atcgcatcgt taacaaggga ctgaagatcg atttcttcct gtatatcctt
    10681 catggcatca ctgaccagtg cgttcagctt gtcaggctct ttttcaaaat caaacgttct
    10741 gccggaatgg gattcctgct caggctctga cttcagctcc tgttttagcg tcagagtatc
    10801 cctctcgctg agggcttccc gtaacgaggt agtcacgtca attacgctgt cacgttcatc
    10861 acgggactgc tgcacctgcc tttcagcctc cctgcgctca agaatggcct gtagctgctc
    10921 agtatcgaat cgctgaacct gacccgcgcc cagatgccgc tcaggctcac ggtcaatgcc
    10981 ctgcgccttc agggaacggg aatcaacccg gtcagcgtgc tgataccgtt caaggtgctt
    11041 attctggagg tcagcccagc gttccctctg ggcaacaagg tattctttgc gttcggtcgg
    11101 tgtttccccg aaacgtgcct tttttgcgcc accgcgctcc ggctctttgg tgttagcccg
    11161 tttaaaatac tgctcagggt cacggtgaat accgtcatta atgcgttcag agaacatgat
    11221 atgggcgtgg ggctgctcgc caccggctat cgctgctttc ggattatgga tagcgaactg
    11281 ataggcatgg cggtcgccaa tttcctgttg gacaaaatcg cggacaagct caagacgttg
    11341 ttcgggtttt aactcacgcg gcagggcaat ctcgatttca cggtaggtac agccgttggc
    11401 acgttcagac gtgtcagcgg ctttccagaa ctcggacggt ttatgcgctg cccacgccgg
    11461 catattgccg gactccttgt gctcaaggtc ggagtctttt tcacgggcat actttccctc
    11521 acgcgcaata taatcggcat gaggagaggc actgcctttt ccgccggttt ttacgctgag
    11581 atgataggat gccatcgtgt tttatcccgc tgaaggcgcg caccgtttct gaacgaagtg
    11641 aagaaacgtc taagtgcgcc ctgataaata aaagagttat cagggattgt agtgggattt
    11701 gacctcctct gccatcactg agcataatca ttccgttagc attcaggagg taaacagcat
    11761 gaataaaagc gaaaaacagg aacaatgggc agcagaaaga gtgcagtata ttcgcggctt
    11821 aaagtcgccg aatgagcaac agaaacttat gctgatactg acggataaag cagataaaac
    11881 agcacaggat atcaaaacgc tgtccctgct gatgaaggct gaacaggcag cagagaaagc
    11941 gcaggaagcc agagcgaaag tcatgaacct gatacaggca gaaaagcgag ccgaagccag
    12001 agccgcccgt aaagcccgtg accatgctct gtaccagtct gccggattgc ttatcctggc
    12061 gggtctggtt gacagtaaga cgggtaagcc tgttgatgat accgctgcct tactgggtgc
    12121 attagccagt ctgaatgacc tgtcacggga taatccgaag tggtcagact ggaaaatcag
    12181 agggcaggaa ctgctgaaca gcaaaaagtc agatagcacc acatagcaga cccgccataa
    12241 aacgccctga gaagcccgtg acgggctttt cttgtattat gggtagtttc cttgcatgaa
    12301 tccataaaag gcgcctgtag tgccatttac ccccattcac tgccagagcc gtgagcgcag
    12361 cgaactgaat gtcacgaaaa agacagcgac tcaggtgcct gatggtcgga gacaaaagga
    12421 atattcagcg atttgcccga gcttgcgagg gtgctactta agcctttagg gttttaaggt
    12481 ctgttttgta gaggagcaaa cagcgtttgc gacatccttt tgtaatactg cggaactgac
    12541 taaagtagtg agttatacac agggctggga tctattcttt ttatcttttt ttattctttc
    12601 tttattctat aaattataac cacttgaata taaacaaaaa aaacacacaa aggtctagcg
    12661 gaatttacag agggtctagc agaatttaca agttttccag caaaggtcta gcagaattta
    12721 cagataccca caactcaaag gaaaaggact agtaattatc attgactagc ccatctcaat
    12781 tggtatagtg attaaaatta cctagaccaa ttgagatgt
//
