LOCUS       NZ_CP025945            13138 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Escherichia coli strain SCEC020023 plasmid p1_020023, complete
            sequence.
ACCESSION   NZ_CP025945
VERSION     NZ_CP025945.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07768275
            Assembly: GCF_002850675.4
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13138)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain SCEC020023
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13138)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 13138)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002850675.4-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 03:24:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,022
            CDSs (total)                      :: 4,900
            Genes (coding)                    :: 4,638
            CDSs (with protein)               :: 4,638
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 82 of 262
            Pseudo Genes (incomplete)         :: 195 of 262
            Pseudo Genes (internal stop)      :: 44 of 262
            Pseudo Genes (multiple problems)  :: 54 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP025945.3.
            On Jul 26, 2018 this sequence version replaced NZ_CP025945.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCEC020023"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p1_020023"
                     /geo_loc_name="China: Sichuan, Yibin"
                     /collection_date="2016-10-27"
     gene            complement(join(12708..13138,1..1036))
                     /locus_tag="C1A20_RS00505"
                     /old_locus_tag="C1A20_00150"
     CDS             complement(join(12708..13138,1..1036))
                     /locus_tag="C1A20_RS00505"
                     /old_locus_tag="C1A20_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017803471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOP flippase family protein"
                     /protein_id="WP_175393456.1"
                     /translation="MNLKSKTIHAVKWNLLSTVCVTGLGMVSLWILSSLFTTTDYGIIS
                     AALILSTVFSIITDFGISNSVIRSEKLNKYELSSLYIINVFLGMIFCLGLFIFSAQLAS
                     LFNGGIELAKQIKIMSFSLIISSFGGQPRALLARELRFDVISRITILSAIFNFVFAVFL
                     SYVYRSPWCVAVSLLVSSSISSVLYIYSSKGLRDIKLQFFTLKEIKKHVSYGSQSVMDS
                     IINQISVNTYPVLMARLISLTAIGGYNISYNISVALFEKLNPVLSHALFPAFSKIQNDA
                     VSLKKTFLKVTMFSSLVNFPMLLGMMLISDYVVTVFFEQKWQFITPIVQILCLVGAARS
                     LDVPVISVLLAKGQMYRNVYLGVFKLALGIPLTWFLGKQYGLTGIVFSFLCIQIVNLVM
                     GYFFQVRPCLNIKVKSYIYAVFVPLIHTLPMLVTGYLLILFLTITSPILSMVVIIFSCM
                     SVYIASILTSPVKEIKEFNAIVLNNILRNK"
                     /name="MOP flippase family protein"
     gene            complement(1317..2120)
                     /locus_tag="C1A20_RS00510"
                     /old_locus_tag="C1A20_00155"
     CDS             complement(1317..2120)
                     /locus_tag="C1A20_RS00510"
                     /old_locus_tag="C1A20_00155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="WP_250211610.1"
                     /translation="MHSRLDLCAATVWSIINQSVQPDKINLWISNEPYLSDHGIYEIPA
                     WVEELNKINNIVNVHYVKNTGPYRKIINAIINSEIDDILVYADDDVIYGRQWLEFLLGQ
                     FYENNCNVAIAARIRIMERNIFNKYKSYSSYSISMKKDKFEKDFIITGVGGCVLSKRMI
                     KPSIIGDDAYLTISPKTDDIWISKMLEASNTKVESSPDALRCVHEILHGYNTLSSINTL
                     TSKNSGLIIKAIRMVKNKFYAYFGLQKTNNDKSIKMVNLHFNGKL"
                     /name="glycosyltransferase family A protein"
     gene            2326..3023
                     /locus_tag="C1A20_RS00515"
                     /old_locus_tag="C1A20_00165"
     CDS             join(2326..2575,2575..3023)
                     /locus_tag="C1A20_RS00515"
                     /old_locus_tag="C1A20_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_250211609.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTLATLERLLGLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKII
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(3240..3662)
                     /locus_tag="C1A20_RS00520"
                     /old_locus_tag="C1A20_00175"
     CDS             complement(3240..3662)
                     /locus_tag="C1A20_RS00520"
                     /old_locus_tag="C1A20_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_101975750.1"
                     /translation="MARVSISEAAQLVKVSRPTIYKMINSGKLSYTSVVKHGKSIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDSESTGINSVVLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3662..3853)
                     /locus_tag="C1A20_RS00525"
                     /old_locus_tag="C1A20_00180"
     CDS             complement(3662..3853)
                     /locus_tag="C1A20_RS00525"
                     /old_locus_tag="C1A20_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4323..4514)
                     /locus_tag="C1A20_RS00530"
                     /old_locus_tag="C1A20_00185"
     CDS             complement(4323..4514)
                     /locus_tag="C1A20_RS00530"
                     /old_locus_tag="C1A20_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021314601.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNDLDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4932..5045
                     /locus_tag="C1A20_RS25410"
                     /old_locus_tag="C1A20_00195"
     CDS             4932..5045
                     /locus_tag="C1A20_RS25410"
                     /old_locus_tag="C1A20_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            complement(5431..6116)
                     /locus_tag="C1A20_RS00545"
                     /old_locus_tag="C1A20_00205"
     CDS             complement(join(5431..5867,5867..6116))
                     /locus_tag="C1A20_RS00545"
                     /old_locus_tag="C1A20_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_250211612.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLPGLEVVVWMTDG
                     WPLYESRLKGELHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYL
                     NIKHYQ"
                     /name="IS1 family transposase"
     gene            6401..6586
                     /locus_tag="C1A20_RS25875"
     CDS             6401..6586
                     /locus_tag="C1A20_RS25875"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_284499092.1"
                     /translation="MDWILLAVAIVLLVVALYSGISYFREKKQSVAHLGDSALFTIIPS
                     YAHRENGLRAFLAGNR"
                     /name="small membrane protein"
     gene            complement(6558..6716)
                     /locus_tag="C1A20_RS25495"
     CDS             complement(6558..6716)
                     /locus_tag="C1A20_RS25495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_214281162.1"
                     /translation="MLLTSGALFSTEITGYAVVCIAMHMRKINQVRFWRVFGWFVACFT
                     GFLPETP"
                     /name="hypothetical protein"
     gene            7559..7780
                     /locus_tag="C1A20_RS00555"
                     /old_locus_tag="C1A20_00215"
     CDS             7559..7780
                     /locus_tag="C1A20_RS00555"
                     /old_locus_tag="C1A20_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016162091.1"
                     /translation="MEFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     gene            7773..8660
                     /locus_tag="C1A20_RS00560"
                     /old_locus_tag="C1A20_00220"
     CDS             7773..8660
                     /locus_tag="C1A20_RS00560"
                     /old_locus_tag="C1A20_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="WP_101975734.1"
                     /translation="MAKFASAARQSAGVIKTLQGGILRSVGTVRNYEQGLTRVAEWMKA
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQARILSSRAYSPDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPRQERTPDP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLIPSPLADRLEHVRLAEPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="integrase"
     gene            8657..9355
                     /locus_tag="C1A20_RS00565"
                     /old_locus_tag="C1A20_00225"
     CDS             8657..9355
                     /locus_tag="C1A20_RS00565"
                     /old_locus_tag="C1A20_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_101975735.1"
                     /translation="MTRTFILLCTGVFAIAYALYELKHMGVYYPDASNPQLFTGWKAVT
                     ELGPAILATAIAATAFSLWLFILFFRSSESRRIQSETDRYKQALREAEEAHTARQNALI
                     RDHNMKTETLHSHYCDAANVLREEYAKREKRWRQALQNAREEARQELEEQTEALNQRAS
                     GLLVQEQTLKARMEEVRKYVAGERHNAMRARLEAEEAEKRRQNASATAERRRRKLEKLQ
                     ATDGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(9362..9766)
                     /locus_tag="C1A20_RS00570"
                     /old_locus_tag="C1A20_00230"
     CDS             complement(9362..9766)
                     /locus_tag="C1A20_RS00570"
                     /old_locus_tag="C1A20_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_101975736.1"
                     /translation="MAKVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLETSE
                     LLRGFGAFAPVSVEQEEEQVSGQRDTVTSDRNELVIYILKQEIEHLNTLLASRDSHIDS
                     LKQAMLLLENREEKRQNTTRPRAWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(9766..9957)
                     /locus_tag="C1A20_RS00575"
                     /old_locus_tag="C1A20_00235"
     CDS             complement(9766..9957)
                     /locus_tag="C1A20_RS00575"
                     /old_locus_tag="C1A20_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_016947206.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            10369..10482
                     /locus_tag="C1A20_RS25415"
                     /old_locus_tag="C1A20_00245"
     CDS             10369..10482
                     /locus_tag="C1A20_RS25415"
                     /old_locus_tag="C1A20_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            complement(10857..11554)
                     /locus_tag="C1A20_RS00590"
                     /old_locus_tag="C1A20_00250"
     CDS             complement(join(10857..11305,11305..11554))
                     /locus_tag="C1A20_RS00590"
                     /old_locus_tag="C1A20_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_250393345.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVCAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSVFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKII
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(11776..12711)
                     /locus_tag="C1A20_RS00595"
                     /old_locus_tag="C1A20_00260"
     CDS             complement(11776..12711)
                     /locus_tag="C1A20_RS00595"
                     /old_locus_tag="C1A20_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_224354408.1"
                     /translation="MMLLSIIIPCYNCESNIEKLLLSIKEQLNETVQLVLINDGSWDAT
                     HDIITKFTSDNPSIKIKYINSINQGAAKARSTGLNYAEGDYVFFCDSDDMISANFISVF
                     TELSNDKPDMIYFCSTVLDSQGNRFKKVHFPLAYIESNSNEVFNYLLKKGQWTAAVWTY
                     IFRKDLVLKSGAIFTDRKVHEDHLFTLRLLAGSEKISICNSELYFQNVTSGSLTKSPKK
                     IDYLTERYKSYKEAREDIIYKFSQESIVLYDRWSMSSLLQILKENKKMVFQGIMHTAIY
                     RVLWKEKLVILDFVKSKVKKKITRSKSLSQ"
                     /name="glycosyltransferase family 2 protein"
     Typing          4694..5045
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.458"
                     /coverage="92.0"
                     /evalue="9.83e-170"
     Typing          10006..10482
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.684"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 taacaggcac atcaagggat cgcgccgccc caaccaaaca taaaatctgt acaatagggg
       61 tgatgaactg ccatttctgc tcaaaaaaaa cagtcactac atagtctgaa attaacatca
      121 ttcccaataa catcgggaaa ttcaccaaag acgaaaacat agttactttt agaaaagttt
      181 ttttcagaga tactgcatca ttttgtatct tggaaaaagc tggaaataac gcatgtgata
      241 aaacaggatt caatttttca aaaagcgcca cgctaatgtt gtaggatata ttatatccac
      301 cgatagcagt caaacttatt aagcgtgcca ttagtacagg atatgtattc acgcttatct
      361 gattaatgat agagtccatg accgactgag aaccatagct gacatgtttt tttatttcct
      421 tcaaagtaaa gaactgtaac ttaatatctc tcaagccttt gcttgaatat atatacaata
      481 cgctactaat agatgaggaa accaacagtg aaacggcaac acaccagggg gaacgataga
      541 cgtagcttaa gaagactgcg aacacaaaat taaaaatagc tgacaggatt gttatcctgg
      601 atattacatc aaatctaagt tctctggcta acaaagctct tggctgtcca ccgaaagacg
      661 atattataag agaaaatgac atgattttta tctgttttgc cagttctatt ccaccattga
      721 ataaggatgc taactgagca ctaaaaataa acaagccgag acaaaatatc atacctaaaa
      781 agacatttat tatatacagt gaagacagtt catatttatt taacttttca gatcttataa
      841 ctgagtttga tataccaaaa tcagttatga ttgagaaaac agtacttaat attaatgccg
      901 cactaatgat accataatct gtcgttgtaa ataagctgga caagatccac agtgagacca
      961 taccaagccc ggttacgcac actgtagaca acaaattcca cttcacagca tgtattgttt
     1021 tcgattttaa attcatattt attgtcagtg atataaaata attacattgg tgtactaaaa
     1081 ggaatttttt atacctttca gcgttagagt ctatcccaaa gtaatgctgc caacctgttg
     1141 gtggccattt gaatctgtag ccattcaatc cttgtaatat caggtgatcc tggcgtcagt
     1201 gaaaatgcga aatcaataaa ttggcatcat tacccaaaaa gcaataacaa tttactaacc
     1261 actacagttt actgataaca tttttttttc agacaatccc tgatgagaaa gatcatttat
     1321 aatttaccat taaaatgcaa attaaccatc ttgatggatt tgtcattgtt tgttttttgc
     1381 aacccaaaat atgcatagaa tttgttttta accatcctga ttgcctttat aattaaaccg
     1441 ctatttttag atgttaatgt atttattgag ctaagtgtgt tatagccatg caatatttca
     1501 tgaacacatc gcagtgcatc tggggatgac tcgaccttcg tattggaagc ctctaacatt
     1561 ttactaatcc agatatcatc tgtcttaggt gaaatagtta aatatgcatc atctccgata
     1621 atgctaggct taatcattct cttacttaaa acacaacccc caacgccagt tattataaaa
     1681 tccttttcaa atttatcctt tttcatacta atagaataag agctataaga tttgtacttg
     1741 ttaaaaatat tcctctccat tattctaatc ctagcagcaa ttgccacatt acagttgttc
     1801 tcataaaact ggcctaatag gaactcaagc cactgccgac catatattac atcatcatca
     1861 gcatatacaa gaatgtcatc aatctccgag ttaataattg cgttaattat tttacggtat
     1921 gggccggtat tttttacata atgtacattc actatgttat ttattttatt tagctcctca
     1981 acccaagcag gtatctcata tatcccatga tctgacaaat atggctcatt tgatatccaa
     2041 aggtttattt tatctggctg aacactctgg tttataattg accacactgt ggcggcacat
     2101 aaatccagtc gagaatgcat tgtagttaaa ttaactgata tcatgctacc ctttaaagaa
     2161 aaaattaatt taatgttata ttatatgtaa aaaggttaca tattcaatat gtaggacatt
     2221 ttttcgaaaa aatcagtttt tttcctgatg aaccttcctc cgcgttgccc ggtaatgctg
     2281 ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct
     2341 atcagctgtc cctcctgttc agctactgac ggggtggtgc gtaacggcaa aagcactgcc
     2401 ggacatcagc gctatctctg ctctcactgc cgtaaaacat ggcagctgca gttcacttac
     2461 accgcctctc agcccggtac gcaccagaaa atcattgata tggccatgaa tggcgttgga
     2521 tgccgggcaa ccgcccgcat tatgggcgtt ggcctcaaca cgattttacg tcacttaaaa
     2581 aactcaggcc gcagtcggta acctcgcgca tacaaccggg cagtgatgtg attgtctgcg
     2641 ctgaaatgga cgaacagtgg ggctacgtcg gtgctaaatc acgtcagcgc tggctgtttt
     2701 acgcgtatga caggctccgg aagacggttg ttgcgcacgt attcggtgaa cgcactctgg
     2761 ccacgctgga gcgtcttctt ggcctgctgt cggcctttga ggtcgtggta tggatgacgg
     2821 atggctggcc gctgtatgaa tcccgcctga agggaaagct gcacgtaatc agcaagcgat
     2881 atacgcagcg aattgagcgg cataacctga atctgaggca gcacctggca cggctgggac
     2941 ggaagtcgct gtcgttctca aaatcggtgg agctgcatga caaaatcatc gggcattatc
     3001 tgaacataaa acactatcaa taattttgag tcattaccga ccttacagaa ggtaatactg
     3061 cgtaatttaa taacaactaa ctaactgata ttaatgagtt atcttcaaaa ttttaatttt
     3121 gcgtggggtg tgggtatggg tatttttagt ggtgaggagc gttcagagat ctggcggtat
     3181 ctgggaaggt tcaggaacgc cccgggatga ccggagagga aaactgaagc atggcgggat
     3241 caggattttt tccagaattt ccaccacggg gaatggggtg gtgaagaagg ttcgtgcttg
     3301 tgctccagaa gctgcatagc ctgacgaagg gaatcaatat gctcatctcg agccttaaca
     3361 gcccctttaa gaccatcgtt ttcagcttca agtaaagcga ttctatgttg taaatcatgt
     3421 aaaacaacgc tgttaatacc tgtagattct gagtcacttt ttacatcata ttttacagta
     3481 tcgatcacac catcaagaga accaaacacc ctaataagct ctgaagtatc aataacttta
     3541 atagacttcc cgtgctttac aacagaggtg taacttaact tcccactgtt aatcatcttg
     3601 taaatcgtgg ggcgactaac cttaacaagc tgtgccgctt cactaataga cactctggcc
     3661 atcagttacc atcctgtaat tcatccagtc tagaggacag tgtacggaaa agatgctccg
     3721 catcatcgtg gagtttctca caaaggtcgg cctcgacatc gagatcaagc tcgttaagct
     3781 tctccagcag gagcagagac tgattctgga tgaatttagc catgttcagg gcagttttct
     3841 gttgtttcgt cataagggcc ctcagagcat cagtgctcag tgtaaaccgg taagggggtc
     3901 ggatgtacaa atggttcaga gaacggatct gaggtaatcc tgtagttatt ttgtagttta
     3961 tctgtagtca tcgcactaaa aatcaggatt aaatcatcaa cttttgtagt cttcctgtag
     4021 tttatctgta gtccggagaa ataatccgtt catgcgagca tgaacgaggt cgctaaggac
     4081 gctttttcgt caccgatgct ccttaacctg cgcagtacac ccggccagaa tgataatttt
     4141 ttgcccctta aatggtcata cacctaacgc agctgcgatg ttcatcgcaa cttcatgaaa
     4201 cataaataaa aaagaaaaaa tacagggatt ctgggtatta ccggggtaat aatgttaagc
     4261 gccaagacaa tgaaaattaa ggcgatgccg gtattactcc ggtaataccc agaacggagc
     4321 cattacgcac cgtcctgctg ctcattcagg cgcgctgaca ggcttcggta aaggcgttcc
     4381 gcatcctcat ggagtttctc gcagaaatca gcctcatgat ccagatccag gtcattaagc
     4441 ctgtccagaa gaagcaggga ctgatcctgg atgaatttag ccatgttaac ggcagtcttt
     4501 tgttgtttgt tcatgaaagc cccggcgcga aagtgcttac tgtacgttcg gatggttttc
     4561 cgcttcctcg ctcattcgct cactccgttc ggtcatgagt ctgcggcgag cggagggaag
     4621 ataagcagat aacgtaacgg taagcataac gaaagctaag gacaagggcg aaaaggccac
     4681 gccccgccgg ggcgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
     4741 gctcaaatca gtggtggcga aacccgacag gacttaaaga taccagacgt ttccccctgg
     4801 tggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     4861 atggccgcgt ttatctcatt ccacgcctga cactcagttc cgggaaggca gttcgctcca
     4921 agctggactg tatgcacgaa ccccccgttc agtccgactg ctgcgcctta tccggtaact
     4981 atcgtcttga gtccaacccg gaaagacatg caaaagcgcc actggcagca gccactggta
     5041 actgatttag aggagatagt cttgaagtta tgcgccggtt aaggctaaac taaacgaaca
     5101 gttttggtga ctgtgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga
     5161 gaaccttcga aaaaaccgcc ttgcagggcg gttttttcgt ttactgagca agagattacg
     5221 atgctaaaga aaggatctca agaggatcct gttccatatg cctacccctg aagtgaaacc
     5281 agcaccactc cgcgacacca accatcaaaa taaaactatt taactcattg tttttgataa
     5341 gaaaaaacat atataatcat gatccataat catccaacgc ggttgacctg cccccaggat
     5401 tagatacaac cttcaggtaa tgactccaac ttactgatag tgttttatgt tcagataatg
     5461 cccgatgact ttgtcatgca gctccaccga ttttgagaac gacagtgact tcctgcccag
     5521 ccttgcaaga tgctgcctca gattcaggtt atgccgctca atgcgctgcg tatatcgctt
     5581 gctgataacg tgcagttctc ccttcaggcg tgattcatac agcggccagc catctgtcat
     5641 ccataccacg acttcaaggc ccggaagacg ctccagcgtg gccagcgtgc gttcaccgaa
     5701 gacgtgcgcc acaaccgtcc tccgtatcct gtcatacgcg taaaacaacc agcgctggcg
     5761 tgatttagcg ccgacgtaac cccactgttc gtccatttca gcgcagacaa tcacatcact
     5821 gcccggttgt atgcgcgagg ttaccgactg cggcctgagt tttttaagtg acgtaaaacc
     5881 gtgttgaggc caacgcccat aatgcgtgca ctggcgcgac atccgacgcc attcatggcc
     5941 atatcaatga ttttctggtg cgtaccgggc tgagaggcgg tgtaagtgaa ctgcagctgc
     6001 catgttttac ggcagtgaga gcagagatag cgctgatgtc cggcagtgct tttgccgtta
     6061 cgcaccaccc cgtcagtagc tgaacaggag ggacagctga tagaaacaga agccactgga
     6121 gcacctcaaa aacaccatca tacactaaat cagtaagttg gcaccatcac ccgtgatttg
     6181 ttatataccc accacggggg cattagtttt tttatttttt tgatgatatg tacaaaatca
     6241 gtgattagga ataatcttag cagccttaac aaacacatac aaaaacagtg gttatgtttc
     6301 atctcaccac aataagtaac gacagtatca tgctaaaaaa agatgatatt atctttatga
     6361 aatcataacg ggctgttttt gttaaattaa tcaggcactt atggactgga ttttacttgc
     6421 tgtcgccatt gttctacttg ttgtagctct ctactcagga atctcatact tcagagaaaa
     6481 aaaacaatca gtcgcacatt taggcgatag cgccctattt accataatcc cttcttacgc
     6541 gcaccgcgaa aacggcctca gggcgtttct ggcaggaaac cggtaaaaca ggcaacaaac
     6601 cacccgaaaa cccgccagaa acgcacctga tttattttac gcatatgcat ggctatgcac
     6661 accaccgcat agccggtgat ttcggtcgag aaaagggccc cggacgttaa aagcataaaa
     6721 gaacgggcca tccaggcccg tcaaatgagg acaactctgt aaaaggaccg tcgcacctgg
     6781 tgcggtgccg gcgacaagaa aaagatagct catatcagcc cctcaccgga gaaaaatatt
     6841 tcctggccac ctcaaccgcc acacgctgtg ttcccgttaa ctcgcgcaga taacgcagga
     6901 acatcctgtt ttcctctgca tgcgcctcct cactgccctg tgcgtggtat tccactatcg
     6961 cgttcaccac ggactcaccg acctgcatcg tctgacctca tctgcacttt catcacaccg
     7021 tttcagatcc cgcctcctcg ccatgatgtg aaacggtgac cgggcaggca aattttttgt
     7081 ctgtccggtc accgttggcg gggtccgggg cggtcagtcc cggtgttttt tattccgcca
     7141 aatcagcacc tgcgacaccc gctatgtacg acgcataaat gcgcctgtac atagcgggtg
     7201 tcgctattga gcggagtcag acgcaacatt taaaaaacaa cattcacacg cacgaaaaac
     7261 ctggctagta tttagtcaaa ccatgagccc gcgcactctg acgcgcacct gcctaccggc
     7321 agcaggtaaa acagccgggt ccctccgggg atactgtacc gggcagccca atccgagaac
     7381 actgagacaa cagcccaaat ctgtcagacc cacaaccggg tcaagtcatt ctctgactaa
     7441 agagacgttg agccgcgagc agtccgggct taaccacgtt acggtttaaa cgagggcgat
     7501 cggcgcaggc cgcagtggat ttccactggc gttcttacaa ctcaacaccg gagttcctgt
     7561 ggaatttgat accgccgccg aactggccgc cctgcaggcg cagacccgcc gtatccgcca
     7621 ggtccgctac cgtccctccc gccttgaccg ctataccggc gaactgctga gcctgtacca
     7681 ggccggggcc agtgcagcgg agctgcaacg ctggctccgg gcccggcgaa tcaaagtggt
     7741 gctctcaacc gtcacccgct ggctggaaaa aaatggctaa attcgccagc gctgcccgcc
     7801 agtcggctgg cgtgataaaa accctccagg gcggtatttt gcgctctgtc ggcacggtcc
     7861 ggaattacga gcagggcctg acccgcgtcg cagaatggat gaaagccaac cggattaatc
     7921 acggcgattt acgcacactg acaccggcgc aggccatcac ctacctggag cagcgcgggg
     7981 agcaggtcgg ccagaaaacg ctcgatatgg aacgccaggc tatccaggcc atgatgatcc
     8041 acgtgacgca gaccctccct gccggcgaac acctgccggt ggtgaaatcg gagcaggccc
     8101 ggatactgag cagccgggcc tacagcccgg accaggtggc cctgattgcc gctgcacaac
     8161 agcccgccaa cgctctggcc accgcgattg ccctggccgc cggattacgc gcccatgaac
     8221 tgctgaccct cgcgccccgc caggagcgta caccggatcc ccgtccggca gcggacagta
     8281 aatttgccgg tcgggagggt gtgatttaca ccgttcaggg caaaggcggc ctggtcaggg
     8341 aagtcctgat accgtccccc ctggcagacc gccttgaaca cgtccggctg gcagaaccgg
     8401 tccgggtgac cgatcgcggc gtgttctacc agagtcagta cgccattggc ggaggacagc
     8461 gctggtcgaa cgccttcagt gccgcctcca tgcgtaccct gggctggtca cggggggcac
     8521 acggcgtgcg tcacagctac gcccagcaac ggatgcagga gctgcaaaaa ctcggtctgg
     8581 ttcgcgatat ggccctgcgc accgtcagcc aggaaatggg ccattttcgt ccggaaatca
     8641 cggagacata cctgcgatga caagaacgtt tattttattg tgtaccggtg ttttcgccat
     8701 cgcgtacgcc ctgtatgagc tgaaacatat gggcgtttac tatccggatg ccagcaatcc
     8761 gcagctgttc actggctgga aggcggtcac cgaactcggt cccgctatcc tggctaccgc
     8821 gatcgcagcc acagcgttct cgctgtggct gttcattttg tttttcagaa gctcagagtc
     8881 ccggcggata cagagcgaga cagacaggta taagcaggct ctgagggagg cagaagaggc
     8941 gcataccgcc agacaaaatg cccttatcag ggatcacaac atgaagacag aaaccctgca
     9001 cagtcactat tgtgatgcgg cgaatgtact gcgggaagag tatgcgaaaa gagaaaagcg
     9061 ctggcgtcag gcgttgcaga atgcccggga agaagccagg caggagctgg aggaacagac
     9121 ggaggcactg aatcagcgcg cgtccggcct gctggtacag gagcagacac tgaaagccag
     9181 aatggaggaa gtcagaaagt atgttgccgg agagcggcat aacgccatgc gggcccgtct
     9241 ggaggcagag gaagcagaga aaaggcgaca aaatgccagc gcaacagcgg aacggcgcag
     9301 acggaaactg gaaaaactcc aggccactga cggaacagca gggcaaaaac actgaggcat
     9361 ttcaggactg ccagaaacgc caccaggcac ggggccgggt cgtgttctgc cgcttctctt
     9421 cccggttctc cagcaacaac atcgcctgtt tcagactgtc gatatgtgaa tctctggagg
     9481 caaggagggt gttcaggtgt tcaatttcct gtttcagaat gtaaataacc agttcattac
     9541 ggtcagaagt gactgtgtca cgctgtccac tgacctgttc ttcctcctgt tcaacggata
     9601 cgggcgcaaa ggcaccaaac ccccgcaaaa gctctgaagt ctcgagcatt ttcgcattcc
     9661 gttcaccccg acaggtggac agctcaccgg ctttaattaa cctgtgcagg gttgtcctgc
     9721 tcttacctgt caggcgtgca gcctcactga tcgacacttt cgccatcagt caccctcctg
     9781 tagtgcatcc agtctgaggg acagtgtacg gaaaagatgc tccgcatcat cgtggagttt
     9841 ctcacaaagg tcagcctccg catcgagatc aagctcatta agcttctcca gcaggagcag
     9901 ggactgattc tggatgaatt tagccatatt cagggctgtt ttctgttgtt tcgtcataag
     9961 gggcctcagg gcgtcagtgc tcagtgtaaa acctgtctct tttccgcttc ctcgctcact
    10021 cagtcgctac gctcctgccg ttcggctgcg gcgagcggac gggacacaca gaagagtcat
    10081 cgcgataacc aggaagagaa gagcctgtgg aaaaggccac ggcgttgccg tttttccata
    10141 ggctccgccc ccctgacaag catcacagaa acggacgctc aaatcagcgg tggcgaaacc
    10201 cgacaggact ataaagatcc caggcgtttc cccctggtag ctccctcgtg cgctctcctg
    10261 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgttta tctcattcca
    10321 cgcctgacac tcagttccgg gaaggcagtt cgctccaagc tggactgtat gcacgaaccc
    10381 cccgttcagt ccgactgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
    10441 agacatgcaa aagcgccact ggcagcagcc actggtaact gatttagagg agatggactt
    10501 gaagttatgc gccggttaag gctaaactaa acgaacagtt ttggtgactg cgctcctcta
    10561 actagccagt taccttggtt caaagagccg caaactctac gaaccttcgg aaaaaccgcc
    10621 ccgcaaaggc ggttttttcg tttacagagc aagagattac gacgttcaaa aacggatctc
    10681 aagaagatcc tttcaagatc tacgacgata tcaatgttca ggctcatcaa aaccatcatt
    10741 gactgaagag ctggattggc ccctatattt ccatacactt tttatcactt aacccattac
    10801 tggttcgccg ccgcaggtat tcccgtggtg aacgatatcc cggtaatgac tccaacttac
    10861 tgatagtgtt ttatgttcag ataatgcccg atgattttgt catgcagctc caccgatttt
    10921 gagaacgaca gtgacttccg tcccagcctt gccagatgtt gtctcagatt caggttatgc
    10981 cgctcaattc gctgcgtata tcgcttgctg attacgtgca gctttccctt caggcgggat
    11041 tcatatagcg gccagccatc cgtcatccat accacgacct caaagaccga cagcaggctc
    11101 agaagacgct ccagtgtggc cagagtgcgt tcaccgaaga cgtgcgccac aaccgtcctc
    11161 cgtatcctgt catacgcgta aaacagccag cgctgacgtg atttagcaca gacgtagccc
    11221 cactgttcgt ccatttcagc gcagacaatc acatcactgc ccggttgtat gcgcgaggtt
    11281 accgactgcg gcctgagttt tttaagtgac gtaaaaccgt gttgaggcca acgcccataa
    11341 tgcgtgcact ggcgcgacat ccgacgccat tcatggccat atcaatgatt ttctggtgcg
    11401 taccgggctg agaggcggtg taagtgaact gcagctgcca tgttttacgg cagtgagagc
    11461 agagatagcg ctgatgtccg gcagtgcttt tgccgttacg caccaccccg tcagtagctg
    11521 aacaggaggg acagctgata gaaacagaag ccactggagc acctcaaaaa caccatcata
    11581 cactaaatca gtaagttggc agcatcaccc agaaagacca taaaattagc ctataacggc
    11641 aaatagtaat gctgccaacc tgttggtgac catttgaatc tgtagccatt caatccttgt
    11701 aacatcaggt gatcctggcg tcagtgaaaa tgcgaaatca acaaattggc atcattaccc
    11761 attatacgat aatccctatt gggataggct cttagatctc gtgatttttt ttttaacttt
    11821 gcttttaaca aaatctaaaa tcactaattt ttccttccac aatactcgat atatagctgt
    11881 atgcattatc ccctgaaaca ccattttttt attttctttt aatatttgca gtagcgaaga
    11941 catagaccat cgatcataaa gaacaatgct ttcctgactg aatttgtaaa tgatatcttc
    12001 cctggcctct ttataagact tataacgctc agttaaataa tcaatttttt taggggattt
    12061 agtaagtgaa cctgaagtca cattctgaaa gtaaagctca gaattacata ttgatatttt
    12121 ttcactacca gcgagaagtc tcagcgtgaa taagtgatct tcatgtacct ttctgtctgt
    12181 aaatatagcg ccagatttca atacgagatc tttccggaaa atataagtcc atacagcagc
    12241 tgtccactgc cctttcttca aaagataatt aaaaacttcg ttactattgc tttcgatgta
    12301 tgctaatgga aaatgtacct ttttaaacct gtttccttgc gaatctaata ccgtagagca
    12361 aaaatatatc atatccggtt tatcattact taactctgta aaaaccgata tgaaattagc
    12421 agaaatcata tcatctgaat cacagaagaa tacataatct ccctctgcgt aatttaagcc
    12481 cgttgatctg gcttttgccg ccccctgatt aatactatta atgtatttta tttttataga
    12541 ggggttgtca cttgtaaatt tagttattat gtcatgcgtt gcgtcccatg agccatcatt
    12601 aatcaatact agttgtacgg tttcattaag ctgctctttt attgagagaa gcaatttttc
    12661 gatatttgac tcacagttat aacatggtat gattattgat aataacatca tttattcctc
    12721 agtatattgt ttagaacgat tgcgttaaat tcctttatct cttttactgg agaggtgagt
    12781 atagaagcaa tgtatacaga catacatgaa aaaataatca caaccattga caaaatagga
    12841 gaggtaatag ttaaaaaaag aatcaacagg taacccgtta caagcatagg taaagtgtgt
    12901 ataagcggta cgaagacggc atagatgtaa ctctttactt tgatatttaa acagggtcta
    12961 acctggaaaa aatatcccat aacaagattt actatttgaa tacataagaa tgaaaacact
    13021 atccctgtaa gaccatattg tttacccaga aaccatgtta aaggaatacc aagcgctaac
    13081 ttaaatactc ccaggtaaac atttctgtac atttgccctt tagcaagcaa gacagaga
//
