LOCUS       NZ_CP025579             4572 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 08EU827 plasmid p08EU827_3, complete
            sequence.
ACCESSION   NZ_CP025579
VERSION     NZ_CP025579.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08222595
            Assembly: GCF_004010995.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Bikkarolla,S.K., Nordberg,V., Kabir,M.H., Muller,V., Fritzsche,J.,
            Ambjornsson,T., Giske,C.G., Naver,L., Sandegren,L. and Westerlund,F.
  TITLE     Combining optical DNA mapping and long-read sequencing for full
            genomic characterization of plasmids in a nosocomial resistance
            outbreak
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Sandegren,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2017) Medical Biochemistry and Microbiology,
            Uppsala University, Husargatan 3, Uppsala SE-75123, Sweden
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: DEC-2017
            Assembly Method                   :: CLC Genomics Workbench v. 11
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 57.0x
            Sequencing Technology             :: Illumina MiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 02:51:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,311
            Genes (coding)                    :: 5,175
            CDSs (with protein)               :: 5,175
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 60 of 136
            Pseudo Genes (incomplete)         :: 73 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 20 of 136
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP025579.1.
            Bacteria and source DNA available from Christian Giske, Klinisk
            Mikrobiologi, Baktlab, Karolinska Universitetssjukhuset, L7:01.
            SE-17176 Stockholm.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="08EU827"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p08EU827_3"
                     /geo_loc_name="Sweden: Stockholm"
                     /collection_date="2008"
     gene            32..370
                     /locus_tag="CY656_RS27695"
                     /old_locus_tag="CY656_27830"
     CDS             32..370
                     /locus_tag="CY656_RS27695"
                     /old_locus_tag="CY656_27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_155559896.1"
                     /translation="MAKVTRIQGGQYAVDDGRFIVKNSSGWLVVDANGSHDFGPVPTLT
                     AAKQYVESGTTPLGQHNFSTNYGRRQSKKEFNAYLASEAKNGNPLPAILWFIVLCIICG
                     VVLAVRGY"
                     /name="hypothetical protein"
     gene            complement(531..794)
                     /locus_tag="CY656_RS27700"
                     /old_locus_tag="CY656_27835"
     CDS             complement(531..794)
                     /locus_tag="CY656_RS27700"
                     /old_locus_tag="CY656_27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_089597835.1"
                     /translation="MAVYTSETKQFLDSVSHDKTWWTSRYNPADDVPVSGIYRCTVCGK
                     EITSNKDDPFPPQNHHQHSQSQAIKWQLIVRTDTQGNGCGSK"
                     /name="protein L"
     gene            complement(958..1374)
                     /locus_tag="CY656_RS27705"
                     /old_locus_tag="CY656_27840"
     CDS             complement(958..1374)
                     /locus_tag="CY656_RS27705"
                     /old_locus_tag="CY656_27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283459.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_057103209.1"
                     /translation="MSDQKNNLDLQKQTLESKWGKEALSMGWSAIPTSLFFLQGALNMT
                     PTTFNVLLHLIIHWWKPYDWPHPSQESIAVRMGVSSRTVQRSLQELENYGLLTKIRTSK
                     DNPKYKGRNIYDMSLLVKSLNILTPDLNAKLNEK"
                     /name="helix-turn-helix domain-containing protein"
     gene            1722..2396
                     /locus_tag="CY656_RS27710"
                     /old_locus_tag="CY656_27845"
     CDS             1722..2396
                     /locus_tag="CY656_RS27710"
                     /old_locus_tag="CY656_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="WP_096908159.1"
                     /translation="MRTIVYVDGFNFYHGLVKRSGYKWLDLYKLFEEQLLPIRARESQL
                     IQVKFFTAEIKAKFASQGELAHQAQQAYHRALVQIYGDKLQIIKGYYSERQECAMTFNE
                     GQPPSKTDRTTVWKLEEKQTDVRMALEMYRDAIRGEAEQLVLCTNDSDMIPPIEMIKAD
                     RPELKIGIVVPREQDTSRVISSLASLGDWSLPYVSYEALRLALLPDKVPTRRKPILKPN
                     HW"
                     /name="NYN domain-containing protein"
     gene            3268..3459
                     /locus_tag="CY656_RS27720"
     CDS             3268..3459
                     /locus_tag="CY656_RS27720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127900087.1"
                     /translation="MKPSNNLSAIGYHPMPSEYDEQLGSTDTQLPCEYDLFDLMAQVTE
                     ENLHAPVDFGPPVGREAL"
                     /name="hypothetical protein"
     gene            3842..4210
                     /locus_tag="CY656_RS27725"
                     /old_locus_tag="CY656_27855"
     CDS             3842..4210
                     /locus_tag="CY656_RS27725"
                     /old_locus_tag="CY656_27855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001273382.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPENGFFLSENDFMRAEEE
                     SQRMVESFRDSLGRKKGF"
                     /name="hypothetical protein"
     Typing          3103..3317
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="88.532"
                     /coverage="100.0"
                     /evalue="7.33e-68"
     Typing          complement(2639..3127)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.129"
                     /coverage="94.0"
                     /evalue="0.0"
ORIGIN
        1 aaagcggcct gatagacatt aggagaatgt attggcgaag gtcactagaa tccagggtgg
       61 acaatatgca gtggatgatg ggcgttttat tgtaaaaaat agctcaggct ggcttgtggt
      121 tgatgccaat ggttctcatg attttggacc tgttcctacg ctaaccgctg cgaagcaata
      181 tgttgagtct ggaaccactc cgttaggaca gcataatttt tccaccaatt acggtcgcag
      241 acagagcaaa aaagagttta atgcatatct tgcctctgaa gctaaaaatg gaaacccgct
      301 gccagccatc ctttggttca ttgttctttg cataatctgt ggcgttgtat tggcagttcg
      361 aggttattag gtaaggttga tatgcatatc ctttggtctc tatggactat cgacgtgaag
      421 cctacgactg aagcccgcaa agcgggcttt ttcatgcccg gcggacaacg gatgttatgg
      481 taaatccagc tgattggcag gtacaaaaaa agcccctaag ggcttttgag ttatttagaa
      541 ccacaaccat ttccttgcgt atctgtcctg acaattaatt gccatttgat tgcctgactt
      601 tggctgtgtt gatgatgatt ttgcggcgga aacgggtcat ctttattgct ggttatctct
      661 tttccgcaaa cagtacaacg atagatacct gatacaggta catcgtcagc agggttatat
      721 cgtgatgtcc accaagtttt atcgtgtgaa acactgtcta aaaattgttt agtctcagag
      781 gtgtatacag ccatttacct tgcctttcca aaatgtatag ggtgggggta tcctctttat
      841 aagtatggtt tttttagagt attttcaatc atcgggtgat gattgaaaat actctaaaat
      901 agagatgcga tggtaaatct gaattgtaat ggtttgattt ttaaaattat tccatggtta
      961 cttttcgttc agcttggcat tgaggtctgg cgttagaatg tttagtgact taactaatag
     1021 agacatatcg taaatgtttc gccctttgta cttagggtta tccttgcttg tacgaatttt
     1081 tgtaagcaat ccgtaatttt caagttcttg gagtgagcgt tgaactgttc ttgatgaaac
     1141 tcccattcta accgcgatgc tctcttgaga tggatgtggc caatcatagg gtttccacca
     1201 gtgaataatt agatgaagta gcacattgaa tgttgtgggg gtcatattga gtgcgccctg
     1261 caggaagaaa agggatgtag gaatggctga ccaccccatg cttaatgctt ccttccccca
     1321 tttagattca agtgtctgtt tttgaaggtc taaattattt ttttgatctg acatatacca
     1381 acctgctaag ctgaagtctt ctaagaagac ttcagcttag caggttggta aattaattca
     1441 tataattagt acgacaccag cgcgcttata atatcattat atttaactat acttaatcta
     1501 cactcacctc ttggtagcgc gattaagtta cgcagctccc ttgaggctct aaacctccct
     1561 ttaggtgggc agcgattaag ttacgcgtct tccttcagga gcttgaaacc gccctcacgg
     1621 ttagttttaa ccctgaggaa ctacaactta aactgtttgc taacgttagt ttttgttgtt
     1681 aaaaccgccc ttagaggcgg tttttttatg cgcgaaataa aatgagaaca atagtttacg
     1741 tagatgggtt caatttttat cacggattgg tgaagcgttc gggatataag tggttagacc
     1801 tttataagct ctttgaagaa caactgcttc cgataagagc aagagaatca cagttgattc
     1861 aggtgaaatt ctttactgct gagatcaaag caaagtttgc ttctcagggg gaattggcgc
     1921 accaggctca gcaagcctac catcgagcat tagtacagat ctatggtgat aagctacaga
     1981 taatcaaagg ttattactca gagcgtcagg aatgtgcaat gacctttaat gaagggcagc
     2041 cgccatcaaa aacggataga acaacggtct ggaagttaga agaaaagcag accgacgtta
     2101 gaatggcttt agaaatgtat cgcgatgcga ttcgtggtga ggcagaacag ttagttctct
     2161 gcactaacga ctcagatatg ataccgccaa tagaaatgat aaaagccgat cggcctgagt
     2221 taaaaatagg aatcgtagtg ccaagggaac aagatactag tcgagtgatt agtagtctgg
     2281 ctagtttagg ggattggagc ttgccatatg tatcctatga agccttaagg ttagctttat
     2341 tgcctgataa agtccccact aggcggaagc caatactgaa gccaaatcac tggtaaacag
     2401 ccggatcttc ttgtgatcct tttactctcg tcgtaatctc atgtcctgca aacaaaaaaa
     2461 ccaccgctac caacggtggt tcgtttgccg gaatcaagag ctaccaactc ttttttccga
     2521 aggtaactgg ctttcacgga gcgcagatac caaaactgtc cttccagtgt agccgcagtc
     2581 aggccaccac ttcaagaact ctgtagaagc actctactac tctgctccgt gaatcctgtt
     2641 accagtggct gctgccagtg gcgctttgtc gtgtctttcc gggttggact caagatgata
     2701 gttaccggac aaggcgcagc ggtcggactg aacggggggt tcgtgcatac agtccagctt
     2761 ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagataaa cgcggccata
     2821 acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagctac
     2881 cagggggaaa cgcctgggat ctttatagtc ctgtcgggtt tcgccgccac tgatttgagc
     2941 gtcagatttt gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc agcgccgtat
     3001 cctttttccg gcctttcctg acttgcgtgc catcttttta tcttgtcatt cgtgagcagg
     3061 taacgcccgc cgcagtctca cggcaggagc gtagcgaccg agtgagcgag gaggcgtcat
     3121 gtcaccgtta cctgcatttt gcacttacgt cctatagccg cattttccct tgcaacatga
     3181 ataactttcc tgaattacca ctccgaccta acatattcag cctgtataca ctccgctagc
     3241 gctacgtgac tggttcaggg ctgcgccttg aaaccctcta acaacctctc agctattgga
     3301 tatcacccca tgccatctga atatgatgaa caactgggtt cgactgatac gcagcttccc
     3361 tgcgaatatg acctttttga tttaatggcg caggttactg aagagaacct gcacgccccg
     3421 gtggattttg ggccacctgt cggccgtgaa gcgttatagc tttttcgctt acccccgtac
     3481 gtgtaatacg ccggtattac atcttatttc tttattttca tgtgattaca ggtggttgta
     3541 atacgttgcg tattacataa ataaacggga aattcagttt ttacctttta attcatgcaa
     3601 ttgcgatgaa aatcgcggtt gcgtatggtg tatggccatt taagggataa tgtaacctga
     3661 cttttaaaga atgaatctat ctttggcagc gcatgagatc gataaaaagc gttccacatt
     3721 aggcggttca tggatacatg aaccggaaag gacaaaaata agacaattgt aagacatttt
     3781 ttacgtttta ctcttatttt tggactatta tgagacaagt gtaagacatt atgggcggta
     3841 tatggcgaat tttaaccctg gtccaagaac agccattgca gctgtcaggg attccaagaa
     3901 cgatatagaa acctggaaga aaaatggtat ccgtagggaa gagattgtga ctgtgctgaa
     3961 tgaacgttac ggcctgaata tctcactggt tgcgtttgat aaggctcttt acagattgag
     4021 aaaaagtaca gatgaaccgt ctcttgctga tgtggattct gtgtctgctg cagaaacgaa
     4081 tttctctgaa gccagtgggc ctgaaaatgg tttttttctg tccgagaatg acttcatgcg
     4141 agcagaagaa gaatctcaaa gaatggttga gtcattcaga gatagccttg gcaggaagaa
     4201 aggattctga ttttgaattc taagtagtga aatagtggat cctctactcg aaattgtcgg
     4261 acacctgcaa ttacctaccg tgccttacgg cttggtcaat gccttacggc cacgcaataa
     4321 attgcgtccc cgttcaccgt tttcggataa gaagaacatc ggaaacagga cttaccccgg
     4381 ctgaatggtg tgaaattctg cgctatgcac ttgcgcgcat actcatgcat gccgtaaaaa
     4441 cagagcctgc gcgtttctgg cgggttttcg ggtggtttgt tgcctgtttt accggtttcc
     4501 cgtcagaaac gctctcagag cctctcaggc ggtgcacgca acagatgtta tggtaaatac
     4561 aatgagctgc tg
//
