LOCUS       NZ_CP025481             3459 bp    DNA     circular CON 17-JUN-2024
DEFINITION  Staphylococcus aureus strain 2148.N plasmid p2148.N, complete
            sequence.
ACCESSION   NZ_CP025481
VERSION     NZ_CP025481.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04966148
            Assembly: GCF_001717665.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   LaBreck,P.T., Rice,G.K., Paskey,A.C., Elassal,E.M., Cer,R.Z.,
            Law,N.N., Schlett,C.D., Bennett,J.W., Millar,E.V., Ellis,M.W.,
            Hamilton,T., Bishop-Lilly,K.A. and Merrell,D.S.
  TITLE     Conjugative Transfer of a Novel Staphylococcal Plasmid Encoding the
            Biocide Resistance Gene, qacA
  JOURNAL   Front Microbiol 9, 2664 (2018)
   PUBMED   30510541
  REMARK    Erratum:[Front Microbiol. 2020 May 14;11:877. doi:
            10.3389/fmicb.2020.00877. PMID: 32477301] Publication Status:
            Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   LaBreck,P.T., Rice,G.K., Paskey,A.C., Elassal,E.M., Cer,R.Z.,
            Law,N., Schlett,C.D., Bennett,J.W., Millar,E.V., Ellis,M.W.,
            Hamilton,T., Bishop-Lilly,K.A. and Merrell,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2017) Microbiology and Immunology, Uniformed
            Services University, 4301 Jones Bridge Road, Bethesda, MD 20814, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 96.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001717665.1-RS_2024_06_17
            Annotation Date                   :: 06/17/2024 00:26:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,726
            CDSs (total)                      :: 2,644
            Genes (coding)                    :: 2,579
            CDSs (with protein)               :: 2,579
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 33 of 65
            Pseudo Genes (internal stop)      :: 15 of 65
            Pseudo Genes (multiple problems)  :: 15 of 65
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP025481.1.
            Bacteria and source DNA available from D. S. Merrell.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="2148.N"
                     /isolation_source="Nose from military trainee"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="p2148.N"
                     /geo_loc_name="USA: Ft. Benning, Georgia"
                     /lat_lon="32.3913 N 84.8229 W"
                     /collection_date="2011-10-13"
                     /collected_by="Uniformed Services University"
     gene            1..996
                     /locus_tag="A7U44_RS14160"
                     /old_locus_tag="A7U44_02835"
     CDS             1..996
                     /locus_tag="A7U44_RS14160"
                     /old_locus_tag="A7U44_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199887.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EEVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANIKAI
                     KPNKKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDAEDGNLINTSDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            2286..2903
                     /gene="cadD"
                     /locus_tag="A7U44_RS14165"
                     /old_locus_tag="A7U44_02840"
     ARG             2287..2900
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            2922..3269
                     /locus_tag="A7U44_RS14170"
                     /old_locus_tag="A7U44_02845"
     CDS             2922..3269
                     /locus_tag="A7U44_RS14170"
                     /old_locus_tag="A7U44_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119690.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     Typing          2..996
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcaatata atactactag aagtataacc gaaaatcaag ataataaaac gttaaaagat
       61 atgacgaaaa gtgggaaaca acgcccatgg agagaaaaga aaatagataa tgtaagctat
      121 gcagatatac tagaaatttt aaaaatcaaa aaggctttta atgtaaaaca atgtggtaat
      181 attttagaat ttaagccaac tgatgaaggt tatttgaagt tacataagac atggttttgt
      241 aaatcaaaat tatgtccggt ttgtaattgg agacgtgcta tgaaaaatag ttatcaagct
      301 caaaaagtga ttgaagaagt aattaaggaa aagccaaaag cacgttggtt gtttttaaca
      361 ctttcaacaa aaaatgcgat agatggagat actttagaac aaagtttgaa gcatctaact
      421 aaagcatttg ataggttgag tagatataaa aaggttaaac aaaatcttgt tggatttatg
      481 cgttcaacag aagttaccgt taataaaaat gacggtagtt ataatcagca tatgcacgtt
      541 ttgttatgtg ttgaaaatgc atattttaga aaaaaagaga attatataac tcaagaagaa
      601 tgggttaatt tatggcaaag agcattgcaa gttgattatc gacctgttgc taatattaaa
      661 gcgatcaaac caaataaaaa aggcgataaa gatattgaat ctgcaatcaa agagacctca
      721 aaatattcgg ttaaatcatc tgatttttta actgatgatg atgaaaaaaa tcaagaaatt
      781 gtaagtgatt tagaaaaagg tttgtatcga aagcgtatgt taagttatgg tggattgctg
      841 aaacaaaagc ataaaatttt aaacttagac gatgctgaag atggaaattt gattaataca
      901 agtgatgaag ataaaacaac agatgaagaa gaaaaggcac attcgattac ggcaatttgg
      961 aattttgaaa agcaaaatta ttatttaagg cattagtgat aactgatgtc tttttttatt
     1021 acttttttgt aaaaaagtgc tgtcttattt ttgtgacaaa ttctgtatgt agtgtcacaa
     1081 aactgtgaca aacgcaatat attgtgtcac aaaagtgtga catttcgtct ttttatgccc
     1141 ccatgcaaaa agatcgacga agtcttgaaa tatctggctt tgccagatac ctcatctata
     1201 gatggggtca tattttcctt atgctcttac ggagttctta gagaaaaatt gaaatttctc
     1261 gatttttgat aaaaacgccc tgcaggaatt tagaaaaaca tggggaagtt ttaaagaatt
     1321 ttatgctcat ttttaatttg catgtaaatg ggcagtgtct aaaaaactcg acactggttt
     1381 tactcgaatt ttagtttgtg aaattaagat atatttattt ggctcatatt tgcgttttaa
     1441 aagtttatgt aggtttttag gtataaaact atatgattta cccataaatc tttaaaatga
     1501 cctcttaaaa ttcaaaataa aggcatttaa aaattaacaa aataaaaaag ctactagcaa
     1561 aaaactagta gcttttgtct tgatgtagta ttaattttta tatctgcgcc acaacgccga
     1621 tttcttaaaa cgaatataaa aaatcgacga aggtcgatta tggtttttgc acgcgttctt
     1681 ttagaacgca taagtgcgcc cttacgggat ttaactagat tataacgacg aaaaacagac
     1741 ctgtaaagca ataacaatta ggtctaccag gtagataaag tgtacaaagt agaaccgaaa
     1801 aagttattta ttttgaagat ttttcgtaga tataatttgc taataaactt cctaaaactg
     1861 aataaagaaa aatagttaaa ctcatgtaat cactccataa ttctgaagtg tctccatgtc
     1921 tacgcataga cacgcattta tgccgagaaa atttattgtg cgttgagaag aacccttaac
     1981 taaacttgca gacgaatgtc ggcatagcgt gagctattaa gccgaccatt cgacaagttt
     2041 tgggattgtt aagggttccg aggctcaacg tcaataaagc aattggaata aaggaagaac
     2101 ccttaactaa acttgcagac gaatgtcggc atagcgtgag ctattaagcc gaccattcga
     2161 caagttttgg gattgttaag ggttccgagg ctcaacgtca ataaagcaat tggaataaag
     2221 cacctatgat tatttaaaaa tataagttat aatacattca aaaatatttt tgaatgaggt
     2281 gtattatgat tcaaacggtt gttgctgctg ctgttcttta tattgctaca gcagttgatt
     2341 tattagtaat tttattaata ttttttgcta gagcaaagac tagaaaagaa tatagagata
     2401 tttatgttgg tcaatattta gggtctatta ttttaatatt agttagtttg tttttagctt
     2461 ttgtattaaa ttatgttcca gaaaagtgga tattaggttt attgggttta ataccaattt
     2521 atcttggaat taaagtggct atttatgatg attgtgaagg agaaaagaga gctaaaaaag
     2581 aattgaatga aaaaggattg tctaaattag ttggtacggt tgcaattgtt acgatagcaa
     2641 gttgtggcgc cgataatatt ggtttatttg ttccgtattt tgtgacatta agtgttacta
     2701 atttattact tactctgttt gtctttttaa ttttaatttt cttcttggtg tttactgcac
     2761 aaaaattagc taatatccca ggaattggag aaattgttga gaaatttagt cgttggatta
     2821 tggctatcat ttatatagct ttaggtttat ttattattat tgaaaatgac actattcaaa
     2881 caattttagg atttatattt taatttaggg tgtgatttta tatgagttat gaaaatactt
     2941 gtgatgtgat ctgtgtacat gaggataaag ttaacaatgc tttaagtttt ttagaagatg
     3001 ataaatctaa gaaattactt aacattttag aaaaaatttg tgatgagaag aaattgaaaa
     3061 tcatattatc tttgattaaa gaagatgagt tgtgtgtttg tgatatttct ttgatattga
     3121 aaatgagtgt tgcttcaact tcacatcatt taaggctttt atataaaaat gaggtacttg
     3181 atttttataa agatggaaag atggcatatt attttattaa agacgatgaa ataagagagt
     3241 ttttctctaa aaatcatgag ggtttttgaa acgagtgaaa cgagtttctt cttgtcttga
     3301 tactatatag aaataactcg attttatata tacagctgta actgttgata ttacagcgtt
     3361 taaacgtgtt tttgtgcgtg aaaggaaaat ttgacaataa aaaaccccag ttatattatt
     3421 aaggtgtcga atcttaaata atactggggg tctttttat
//
