LOCUS       NZ_CP025469             3353 bp    DNA     circular CON 25-NOV-2023
DEFINITION  Klebsiella pneumoniae strain JS187 plasmid p187-3, complete
            sequence.
ACCESSION   NZ_CP025469
VERSION     NZ_CP025469.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08180799
            Assembly: GCF_002848565.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Tang,Y., Fu,P., Tian,Y., Zhang,H. and Jiang,X.
  TITLE     The role of Crispr in the dissemination of blaKPC in klebsiella
            pneumoneae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Tang,Y., Fu,P., Tian,Y., Zhang,H. and Jiang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-2017) Huashan Hospital, Shanghai Medical College,
            Fudan University, Department of Laboratory Medicine, No. 12, Urumqi
            Middle Road, Jing 'An District, Shanghai, Shanghai 200040, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5-miseq v. v20150522
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 208.0x
            Sequencing Technology             :: Illumina MiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/25/2023 03:24:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,841
            CDSs (total)                      :: 5,716
            Genes (coding)                    :: 5,548
            CDSs (with protein)               :: 5,548
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 79 of 168
            Pseudo Genes (incomplete)         :: 83 of 168
            Pseudo Genes (internal stop)      :: 27 of 168
            Pseudo Genes (multiple problems)  :: 20 of 168
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP025469.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="JS187"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p187-3"
                     /geo_loc_name="China:Shanghai"
                     /collection_date="2008-12-09"
     gene            267..398
                     /locus_tag="CYE04_RS31455"
                     /old_locus_tag="CYE04_29330"
     CDS             267..398
                     /locus_tag="CYE04_RS31455"
                     /old_locus_tag="CYE04_29330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_101987969.1"
                     /translation="MHEPPVQSDCCAFSGNFQLDSKPKRHDKAPLAAAIGNGVAEQS"
                     /name="replication initiation protein"
     gene            complement(588..989)
                     /locus_tag="CYE04_RS29345"
                     /old_locus_tag="CYE04_29335"
     CDS             complement(588..989)
                     /locus_tag="CYE04_RS29345"
                     /old_locus_tag="CYE04_29335"
                     /inference="COORDINATES: protein motif:HMM:NF017020.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="WP_014343524.1"
                     /translation="MISSKEMLDFARGSYSKFDAAGYICLSEEVEYRTCARSAYYALYH
                     YLKSIADKLPGSYEDVSSHEKVIRKLLNSGDPKLMDLAQKMISTRKTRVRADYHIDKNF
                     GKSEAYKILRIVEKIFKDAELSEGEVLDS"
                     /name="HEPN domain-containing protein"
     gene            complement(986..1777)
                     /locus_tag="CYE04_RS30250"
     CDS             complement(986..1777)
                     /locus_tag="CYE04_RS30250"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_014343525.1"
                     /translation="MVSISPKKAEDEALDILNRVIVEKRNVSEFENVRIINCLDSLRRA
                     RSSSYDEISACYYAQIADIDNLLVSVSNVFSSDCEDSFFNIIYALNNAMRYREIYKYVK
                     DLDLDYNIRDFLESAIKASAFCVDKEMVDKFTTSYANAFHDESSVDFLNHISEINSFFL
                     NHESIKKEMSNYMLDCFDVFSRKSLIKTKEIDGTYGYSHHVFNDEGYEFLAFSFILRGD
                     EIDMDMLIDIEDEFTYEISRIKYDPIIKTKISFNFELGDLS"
                     /name="hypothetical protein"
     gene            complement(2592..3014)
                     /locus_tag="CYE04_RS29360"
                     /old_locus_tag="CYE04_29350"
     CDS             complement(2592..3014)
                     /locus_tag="CYE04_RS29360"
                     /old_locus_tag="CYE04_29350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004207907.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKDIKVID
                     VSELIRVFGSLDGVTDTVKYDVKSDAISTEVNTIGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHDPSSPAHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3014..3205)
                     /locus_tag="CYE04_RS29365"
                     /old_locus_tag="CYE04_29355"
     CDS             complement(3014..3205)
                     /locus_tag="CYE04_RS29365"
                     /old_locus_tag="CYE04_29355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004207910.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDSLQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          11..375
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.278"
                     /coverage="85.0"
                     /evalue="4.93e-157"
ORIGIN
        1 gaaaaaaagg aaaggccacg gcgttgccgt ttttccatag gctccgcccc cctgacaagc
       61 atcgcagaaa cggacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc
      121 aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttgccg
      181 gtgtcattcc gctgttatgg ccgcggtttc tcattccacg cctgacactc agttccgggt
      241 aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gactgctgcg
      301 ccttttccgg taactttcaa cttgattcca aaccgaaaag acacgacaaa gcgccactgg
      361 cagcagccat tggtaacggt gtcgcagagc agagttaacg agagttcttg aagtggctgc
      421 ctaaaaacgg ctacactgga aggacagtat ttggtacctg tgctctgcga aagccagtta
      481 cctcggtcaa aagagttggt agctctgaag accttgaaaa accaccgttg gtagcggtgg
      541 tttttctcta tctggaggtt actacctcct ctttattccc gtcaagctca tgaatccaga
      601 acttcccctt cggaaagttc agcatccttg aagatctttt caacaatacg aagtattttg
      661 tatgcctctg atttgccgaa atttttatct atatgatagt cagctcgaac tctcgtcttc
      721 cttgtggaaa tcatcttctg agctaaatcc ataagttttg ggtcgccaga attaagaagc
      781 tttcgaatca ctttttcatg tgacgaaaca tcttcgtagc tgccaggcaa cttgtcagca
      841 atactcttca aataatgata caacgcataa taagcggaac gagcgcaagt cctatactct
      901 acctcttcac tcaggcagat ataaccagcc gcatcaaatt ttgaataaga gcctctcgca
      961 aaatctagca tttcctttga agagatcatg acaaatctcc caattcaaaa ttaaatgaaa
     1021 tttttgtttt tataattgga tcatatttta ttctagatat ttcatatgta aactcatctt
     1081 cgatatcaat aagcatatcc atgtcaattt catcacctct caatataaag ctgaaagcta
     1141 aaaactcgta accctcatca ttgaagacgt gatgtgaata accataagtt ccgtcaattt
     1201 ctttggtttt tataagagac ttccttgaaa aaacatcaaa gcaatcaagc atataattag
     1261 acatttcttt ttttatgctt tcatgattca aaaagaagga gtttatttca cttatatgat
     1321 ttaaaaaatc cacagaactt tcatcatgaa aagcatttgc gtaagatgtg gtgaatttat
     1381 caaccatctc tttatcaaca caaaaagcag acgcctttat tgctgactct aaaaaatctc
     1441 ttatattata atcaaggtca agatctttta catatttata gatctctcta tatctcattg
     1501 cattatttaa tgcgtaaatg atattaaaga aagaatcctc gcaatctgaa gagaaaacgt
     1561 tagaaacact aaccaataga ttatctatat ctgcgatctg tgcgtaatag caagcagaga
     1621 tttcatcgta agatgaactt cttgcccttc ttaaagagtc caagcaattt ataattcgta
     1681 cattctcaaa ttctgatacg tttctttttt caactataac tctattcaaa atatccaaag
     1741 cttcatcttc tgctttttta ggagatatag ataccatgaa attcactctt ttaatttact
     1801 tttaaatcag gatagcattt tatcggtaca aaaaaaatta tctttaatac aacctattta
     1861 ccataacatc cgttgcgtgc accgcctcag aggccctcag cgcgtttctg acgggaaacc
     1921 ggtaaaacag gcaacaaacc acccgaaaac ccgccagaaa cgcgcaggct ctgtttttac
     1981 ggcatgcatg agtatgcgcg caagtgcata gcgcggaaat tcacaccatt cagccagggg
     2041 aagtcctgtt tccggcgttc ttctcctccg aaaacggtga acggggacgc aatttattgc
     2101 gtggccgtga agcgttgccg gcgcagccgg aaaaccggtt ttcagcacgt tcaggaatcg
     2161 ggttcgtcga cggccggcaa tcacgttatg gcaagtcacc ggacgactta gcacacgatt
     2221 caccggcgat tcgaatcgtg aattgaatca agagtcacca tgaacaaaaa acacccaaag
     2281 tgatgaattg aatcaagagt cgattcatca taacaatatg ataataaagc gtgattcaca
     2341 tatgaatcgt gggcaattcg tcagcgattc gccatagagt cgtccacgac ttggatctgc
     2401 actttgtaac acatcaccgc acatttattt ttcattaaac cattgttgta aataaggaat
     2461 ctgcaaaatt ttaattttgc gtggggtgtg ggtattttta gtggtgagga gcgttcagag
     2521 atctggcgat ttttgggaag gttcaggaac tccccgggat cgccggagag caaaactgaa
     2581 acatggcagg atcaggattt tttccagaat ttccaccacg gagaatgagc cggtgaagag
     2641 gggtcgtgct tgtgctccaa aagctgcata gcctggcgaa gggaatcaat atgctcatcg
     2701 cgtgctttaa cagccccttt aagaccatcg ttttcagctt caagtaaagc gattctatgt
     2761 tgtaaatcat gtaaaccaat ggtgttaacc tctgtagaaa ttgcgtcact ttttacatca
     2821 tattttacag tatcagttac accatcaaga gaaccaaaca ccctaataag ctcggataca
     2881 tcaataactt taatatcctt cccgtgcttt acaacagagg tataacttaa cttcccgctg
     2941 ttaatcatct tgtaaatagt gggacgacta accttaacaa gccgtgcagc ttcactaata
     3001 gacactctgg ccatcagtta ccatcctgta atgaatccag tctcgaggag agtgtacgga
     3061 aaagatgctc tgcatcatcg tggagtttct cacaaaggtc ggcctcagca tcaagatcaa
     3121 gctcgttaag cttctccagc aggagcagag actgattctg gatgaattta gccatgttca
     3181 gcgccgtttt ctgttgtttc gtcataaagg gcctcagagc gtcagtgctc agtgtaaaac
     3241 ctgtctcttt tccgcttcct cgctcactca gtcgctacgc tccggtcgtt cgactgcggc
     3301 gagcggcagg agcatgcata gaaaagctcg agaaaaccga gcggtcaaag gag
//
