LOCUS       NZ_CP025465             5596 bp    DNA     circular CON 25-NOV-2023
DEFINITION  Klebsiella pneumoniae strain F44 plasmid p44-4, complete sequence.
ACCESSION   NZ_CP025465
VERSION     NZ_CP025465.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08180798
            Assembly: GCF_002848585.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Tang,Y., Fu,P., Tian,Y., Zhang,H. and Jiang,X.
  TITLE     The role of Crispr in the dissemination of blaKPC in klebsiella
            pneumoneae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Tang,Y., Fu,P., Tian,Y., Zhang,H. and Jiang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-2017) Huashan Hospital, Shanghai Medical College,
            Fudan University, Department of Laboratory Medicine, No. 12, Urumqi
            Middle Road, Jing 'An District, Shanghai, Shanghai 200040, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: A5-miseq v. v20150522; CANU v. v1.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 278.0x
            Sequencing Technology             :: Illumina MiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/25/2023 03:39:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,944
            CDSs (total)                      :: 5,820
            Genes (coding)                    :: 5,628
            CDSs (with protein)               :: 5,628
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 94 of 192
            Pseudo Genes (incomplete)         :: 104 of 192
            Pseudo Genes (internal stop)      :: 23 of 192
            Pseudo Genes (multiple problems)  :: 28 of 192
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP025465.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="F44"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p44-4"
                     /geo_loc_name="China:Shanghai"
                     /collection_date="2016-04-18"
     gene            complement(join(5326..5596,1..74))
                     /locus_tag="CYE02_RS30715"
                     /old_locus_tag="CYE02_30705"
     CDS             complement(join(5326..5596,1..74))
                     /locus_tag="CYE02_RS30715"
                     /old_locus_tag="CYE02_30705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(64..240)
                     /locus_tag="CYE02_RS31000"
     CDS             complement(64..240)
                     /locus_tag="CYE02_RS31000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            880..1038
                     /locus_tag="CYE02_RS32250"
     CDS             880..1038
                     /locus_tag="CYE02_RS32250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1396..1722
                     /locus_tag="CYE02_RS30725"
                     /old_locus_tag="CYE02_30715"
     CDS             1396..1722
                     /locus_tag="CYE02_RS30725"
                     /old_locus_tag="CYE02_30715"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            1748..2293
                     /locus_tag="CYE02_RS30730"
                     /old_locus_tag="CYE02_30720"
     CDS             1748..2293
                     /locus_tag="CYE02_RS30730"
                     /old_locus_tag="CYE02_30720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            2359..2709
                     /locus_tag="CYE02_RS30735"
                     /old_locus_tag="CYE02_30725"
     CDS             2359..2709
                     /locus_tag="CYE02_RS30735"
                     /old_locus_tag="CYE02_30725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2779..3045)
                     /locus_tag="CYE02_RS30740"
                     /old_locus_tag="CYE02_30730"
     CDS             complement(2779..3045)
                     /locus_tag="CYE02_RS30740"
                     /old_locus_tag="CYE02_30730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3029..3253)
                     /locus_tag="CYE02_RS30745"
                     /old_locus_tag="CYE02_30735"
     CDS             complement(3029..3253)
                     /locus_tag="CYE02_RS30745"
                     /old_locus_tag="CYE02_30735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(3920..4132)
                     /locus_tag="CYE02_RS30750"
                     /old_locus_tag="CYE02_30740"
     CDS             complement(3920..4132)
                     /locus_tag="CYE02_RS30750"
                     /old_locus_tag="CYE02_30740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4139..4648)
                     /locus_tag="CYE02_RS30755"
                     /old_locus_tag="CYE02_30745"
     CDS             complement(4139..4648)
                     /locus_tag="CYE02_RS30755"
                     /old_locus_tag="CYE02_30745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4683..5387)
                     /locus_tag="CYE02_RS32255"
                     /old_locus_tag="CYE02_30750"
                     /pseudo=""
     CDS             complement(<4683..5387)
                     /locus_tag="CYE02_RS32255"
                     /old_locus_tag="CYE02_30750"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(327..403)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          592..989
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(3805..5336)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cagcgctcca gcagctgctg gtactcccca tcggtcaccc ggatacctaa aaaacgggta
       61 cgcttaaccg ccatcctctc ctccgatatc cgcaaaatgt gaccgggctg aacgctcaag
      121 ctctgccatc tgctcccggc ggtaatcttc ctcctgtgcc tgacgcatca gatttgccat
      181 cactgccgga tgccaggatc ttttttttac gacgagcggc ttcttcttcg gcttctgcat
      241 tatccctcct tcgcaaacgc cggacggcac ggaaaagttt tccgggtcgc tccggcgttg
      301 gacaagcgcc agcgcgtcag tggtgttagc gggtttcggg gcgcagccct gaaccagtca
      361 cagcgtttac gctgtgtata ctggcttact atgttggcat gagagcacga ttcatgaaag
      421 tgcctcatgt ggcaggtgtg atatgtggct caggagcgtc cgcaatacca tgcacggaca
      481 gatgaaatga cgcctcctcg ctcactcggt agctgcgctc ctgccgttcg gctgcggcgg
      541 gcgtgaccga ctcacggata acgggataaa gcaaaggcgc tcaaatcaga aagggccgga
      601 gaaaaggatg cggcgttgcc gtttttccat aggctccgcc tcccctgacg agcatcacag
      661 aaatggacgc tcaagtcaga ggaggacgaa acccgacagg acttaaagat gccggtgatt
      721 tctctccggc ggctccctcg tgcgctctcc tgttccgacc ctgccgttta ccggatacct
      781 gttccgcctt tttccctttc gggaagcgtg gcgctttctc atagctcacg ctgctggtat
      841 ctcggctcgg tttaggtcgt tcgctccaag ctgggctgta tgcacgaacc ccccgttcag
      901 cccgactgct gcgccctttc cggtaactat catcttgagt ccaacccgga cggacacgca
      961 aaaacgccac tggcagcagc cattggtaac tggatatata cgagagagat attgagaggt
     1021 cttgaagtgg gtgcctaact gcggctacac tgaaagaaca gttttggtat ctgcggtctc
     1081 gtatacccag ttacccggtt aagcagttcc ccaactgact taaccttcga tcaaaccgcc
     1141 tccccaggcg gttttttcgt ttacagtgca agagattacg cgcagaaaaa aaggatctca
     1201 agaagatcct ttaaatgatc gtcagttctg ttatccccgg agctccatcc atcccacgga
     1261 aaaacacccg ccactgatgc cctcagcgca ctgcacatca tgtacaaacc actccctccc
     1321 tcctttttta ttgcgtttct taatgcgcaa tggtagcttt gtgcgcaaca aaaaacgcaa
     1381 tagcaagggg gggtaatgag agaaagaaca aatatcatgc tagatatagg attaaagcaa
     1441 tccttacaac ggctttcgaa taaaaccaaa atgagtctaa gtgatcttat taaccagaga
     1501 ttatcaggtt cactttcagt tgaagaacgc attggcgaaa agcctgaatg gatgagaaac
     1561 cttgatccct atctcgccaa tcacttttca aatattgagt taccgctccc ttcaccatta
     1621 aaagaatacc aagaaaaaat ggatgagata gattcaatta ttaatgacat gatggttcta
     1681 aaggaatatt tcagagctaa gttttacgac gaaataaaat aggcacgcta tacggaggcg
     1741 gagaattatg ttttttaaac ggtatttaac ggagaaggaa atatacaagg cagcactccc
     1801 aattgttagg cttcaaggga gagttaacac ttcattacgt tttttatcca aatactcaga
     1861 tgtaaacaaa aatggattaa ttgcattgca tcttaattta ttacatttta tctcatacaa
     1921 catacacggt gtagagtctc tgaaaatcac tcaagcaggc ataaacaaag ctgtacaaca
     1981 aagccctcac gcagatgaaa taaaaaatat tgcgcaggca ataggacgca aaggacctga
     2041 gtacatcgaa ggattaatta gaaacccatg ggggaagaaa aatgataatc cactaatatt
     2101 tataatgagc attgaagaaa gtataaaaga aacccttaac tcaggttata tgttatatag
     2161 agtagctccc ataactgagg gtgaagcagc tgctttcatg gatgttctta aacaattatt
     2221 attatttgag gataattttc gcctttttgg tagagacttg gtttataaaa tagaaaaatt
     2281 aaaaatcaga taacaaagta tggtgaacga aaaatgaaag agaggatagt gaaatgtgta
     2341 ataccaagat tctcaactat gtattggtta attgcgttcc tagcatttat attccccggt
     2401 cgatcttttg ctgacggtta caccgcttac taccgatgta atggtactca ggaagcatct
     2461 cttactgtta aagataacaa actaagtatg gttgtaggtc tttctaagtt taataggcaa
     2521 ctggatactt atagattcaa agaaaaggga atcgaagtag taatgcagag agctgcttct
     2581 gatgataact ctgaggccat aatgtctttt attatgcctg acgttaacaa tatgacctta
     2641 ttagttaaaa ataacggcta cattatcgtt aagacaacct gctggttaac taaaaccgac
     2701 aaacagtaga acacgtagtt agtcgagtat ttaccataat acctgttgtc ggcatcaaaa
     2761 aagcccgcat agcgggcttc agtcgtaaac ttcgcggcga tgtccaatcg tggcggcaag
     2821 gataacaagc tcgtcatctc ggatctcaca cagaacgcga taatctccca cccgatagcg
     2881 ccagaactcg cccagctcac ctttgagcgg cttccctgac tggcgaggat cggcagcaac
     2941 tgcaatgcgt aaactcataa aatccacaat ccgtcgtgcg ttctgtttgt ccatcttgcg
     3001 taacgatttg agcgcccgat cggaatagtt aatcgtccag accaagtgac ttcctcacgt
     3061 cttcagacga atgcactgct tcatcaccac ggcgaacgcg tgcagcagtt tctgctgaaa
     3121 gataataatc ctccaggtct tccagatgcg ccagtattgc ttcccgcgca taaaacgtct
     3181 ttgttctgcc ggtttgcttc gccagcgagt ccagacggga ctcaatctca tcagataacc
     3241 ggatagcaag catataaacc tcccgaacac catttgctat acatgtatat caacagtacc
     3301 acaatcaacc taaaagaaca aaaaataatc ttgttagccc ctcttgataa tccgtaacgc
     3361 ggtcaatata tctagcttga gggctttcct cttaactatt gaaaggataa cgattatggg
     3421 tcttaagccg ggtccgaaac gaattgccga ttcaacaggg gaacctgata aacgccagcg
     3481 tgacaataaa aaaacgccgg gaaatactga caaattgaaa cccagcaaat cttcaaaaaa
     3541 ataagtcagt agttaaaagc cctccggggc ttttttgtat ctgccgttca cgctgattaa
     3601 acataacacc cgttgcccgc accgccccgg acgggttcgc cgcgaacggg gtggcagtca
     3661 ggtaaaaccg gcaacaaacc acccgataaa ccgccccgtt cgcgacagat aaattttacc
     3721 tggctgcata gccatgcagc caggtgaatg acatccggtt tcagcgtaaa tgcccgatga
     3781 gaaccctgtt ttcctgcatt tcagcccagc gacggaccgt catagcttcg ctgccgggaa
     3841 cgcgcttccg tcagttcctt ttcgtgctgc cgttgctcgt gcgcccgttc tgcggccacg
     3901 gccatctcat gacgctgcac taccggctca aacgctgcag ttgcccgctc aagcgttcca
     3961 cctgctgact caagcgcgtc acgcgctcgc tcagcgccgc gttctcccgt tgcataagcc
     4021 ctgacatttt ccgccattcc ccgaaggcgc tctcccactc gtccagcctt ttcgagtagt
     4081 cctgctgtag ctgctctaat gcgctcagca actgtttttc cagctcggtc atcggcactt
     4141 actccttcac cagtctcagc gcgttctgct tcgaccgccc gttcccgacc gtccagctct
     4201 gcgtcctgtc tacggccgtc cccttcggca gcaccagaaa acgtccgttg ctgtcttcca
     4261 ggtacgtcac gccccaggtc ttcgcgttca gcttcgacag cgctgcgttc tgccggtcga
     4321 tttcggccag atttgacgct atcaagctcc cctgatacca caacaccccg ctcaggctgg
     4381 cgaacagcag tcctgacacc atcgtgatgg tcagccatgt ccgcccgacc atacgcatca
     4441 cgctcaggcg gttcgactgc atggccgcgc tcataccctg ctcgtgggcg cggatggcgg
     4501 cgctgattct cttcccgctt tcgctcagtt ccgctctgac agacttctcg tgctcgctga
     4561 atgcggcttt cagcatttcg ccggtactct gctgctgcgc tttcgatttc tgctctaagt
     4621 ccttcgccag cgttaaaaga ctgttcatag atggctccct taagtcggat gtttcgccct
     4681 ccgtccgggt cggcaatgct gatgctgctc tttgtggtgc gcacgacctc aaaacctgcg
     4741 ctttccagcg cctcagtgac gtcctgacgc gttttaagct cccctgacga ggcgagggcc
     4801 agtaagcccc gcgtaatagc ctctgcggct tcctgcttcg ttttgggtaa cgaagatggc
     4861 gtcaccagcg cgcgccggtt ctccggtgcg ttcgggtcgt gcagccccag tcgcccgttc
     4921 accacggtct gccacgcatc gatgcgcgga cggtctgccc ggtcgtagta cggctggaga
     4981 cgcctgccgg tcagcagttc cgtgttcggt accagaaaat tcagttccag ccgccccttg
     5041 tcccggtgtt caacccacag cacgctgtac tggtctttat cgagtccggg catcagaacc
     5101 cgctcgaagc tcgccatcag cttttcacgc tggccgggcg gtaaatcctg ttcagcaaaa
     5161 gacaggacgc cggaggtgta ctttttggcg tagggcgagg cgtcgataag ctcccggacc
     5221 tcctccggct ttccctgcag gacgatggcc ccgtcgcgct ggcggtcttt ccccagcaga
     5281 taatccaccg gcccggcacc gccgcctcgc cctcgcggat gaaacttaac gatcatcgtc
     5341 tgcccccttt tccagcacgg catgccgcag ccgctcgagt tcggcatcaa tggccatcag
     5401 cgcggccacc acctgaaccc ggtcagcgcc tgaccactgg ccgccgttaa ttttccgggc
     5461 gatctggttg aggttgttcc ccatcccggc aagctggcgc agcaaaaccg gatcgatgga
     5521 cggcagccgc aacgaccgcg ccgggcgcgt atcgagacag gtttcccgca tccacaccgc
     5581 cagttgcctt ccattg
//
