LOCUS       NZ_CP025464            10060 bp    DNA     circular CON 25-NOV-2023
DEFINITION  Klebsiella pneumoniae strain F44 plasmid p44-3, complete sequence.
ACCESSION   NZ_CP025464
VERSION     NZ_CP025464.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08180798
            Assembly: GCF_002848585.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Tang,Y., Fu,P., Tian,Y., Zhang,H. and Jiang,X.
  TITLE     The role of Crispr in the dissemination of blaKPC in klebsiella
            pneumoneae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Tang,Y., Fu,P., Tian,Y., Zhang,H. and Jiang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-2017) Huashan Hospital, Shanghai Medical College,
            Fudan University, Department of Laboratory Medicine, No. 12, Urumqi
            Middle Road, Jing 'An District, Shanghai, Shanghai 200040, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: A5-miseq v. v20150522; CANU v. v1.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 278.0x
            Sequencing Technology             :: Illumina MiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/25/2023 03:39:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,944
            CDSs (total)                      :: 5,820
            Genes (coding)                    :: 5,628
            CDSs (with protein)               :: 5,628
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 94 of 192
            Pseudo Genes (incomplete)         :: 104 of 192
            Pseudo Genes (internal stop)      :: 23 of 192
            Pseudo Genes (multiple problems)  :: 28 of 192
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP025464.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="F44"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p44-3"
                     /geo_loc_name="China:Shanghai"
                     /collection_date="2016-04-18"
     gene            6..536
                     /locus_tag="CYE02_RS30645"
                     /old_locus_tag="CYE02_30635"
     CDS             6..536
                     /locus_tag="CYE02_RS30645"
                     /old_locus_tag="CYE02_30635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(563..742)
                     /locus_tag="CYE02_RS30650"
                     /old_locus_tag="CYE02_30640"
     CDS             complement(563..742)
                     /locus_tag="CYE02_RS30650"
                     /old_locus_tag="CYE02_30640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(768..1196)
                     /locus_tag="CYE02_RS30655"
                     /old_locus_tag="CYE02_30645"
     CDS             complement(768..1196)
                     /locus_tag="CYE02_RS30655"
                     /old_locus_tag="CYE02_30645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(1360..1674)
                     /locus_tag="CYE02_RS30660"
                     /old_locus_tag="CYE02_30650"
     CDS             complement(1360..1674)
                     /locus_tag="CYE02_RS30660"
                     /old_locus_tag="CYE02_30650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1662..1940)
                     /locus_tag="CYE02_RS30665"
                     /old_locus_tag="CYE02_30655"
     CDS             complement(1662..1940)
                     /locus_tag="CYE02_RS30665"
                     /old_locus_tag="CYE02_30655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(2115..2480)
                     /locus_tag="CYE02_RS32040"
                     /old_locus_tag="CYE02_30660"
     CDS             complement(2115..2480)
                     /locus_tag="CYE02_RS32040"
                     /old_locus_tag="CYE02_30660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(2477..2848)
                     /gene="tolA"
                     /locus_tag="CYE02_RS30675"
                     /old_locus_tag="CYE02_30665"
     CDS             complement(2477..2848)
                     /gene="tolA"
                     /locus_tag="CYE02_RS30675"
                     /old_locus_tag="CYE02_30665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(3122..3367)
                     /locus_tag="CYE02_RS30680"
                     /old_locus_tag="CYE02_30670"
     CDS             complement(3122..3367)
                     /locus_tag="CYE02_RS30680"
                     /old_locus_tag="CYE02_30670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            3694..5379
                     /locus_tag="CYE02_RS30685"
                     /old_locus_tag="CYE02_30675"
     CDS             3694..5379
                     /locus_tag="CYE02_RS30685"
                     /old_locus_tag="CYE02_30675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5389..5646
                     /locus_tag="CYE02_RS30690"
                     /old_locus_tag="CYE02_30680"
     CDS             5389..5646
                     /locus_tag="CYE02_RS30690"
                     /old_locus_tag="CYE02_30680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            5731..5880
                     /locus_tag="CYE02_RS30695"
                     /old_locus_tag="CYE02_30685"
     CDS             5731..5880
                     /locus_tag="CYE02_RS30695"
                     /old_locus_tag="CYE02_30685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            7364..9328
                     /locus_tag="CYE02_RS30705"
                     /old_locus_tag="CYE02_30695"
     CDS             7364..9328
                     /locus_tag="CYE02_RS30705"
                     /old_locus_tag="CYE02_30695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            9328..10059
                     /gene="mobC"
                     /locus_tag="CYE02_RS30710"
                     /old_locus_tag="CYE02_30700"
     CDS             9328..10059
                     /gene="mobC"
                     /locus_tag="CYE02_RS30710"
                     /old_locus_tag="CYE02_30700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     BGC             1..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5984..6562)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9329..10056
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 ggattatggc gctgtacatg gtatttattg aggacgtgga agacaaagac ggcgagcgct
       61 gcattatgcc aaccagcgag catctggaag acatggtggt gctcgcctgg cagaccgggg
      121 cgtatgtttt ctgttttgtc gaccgggccc agcatctggc gatgatgaaa tccatcggga
      181 tgtatgacga gcggctcaac agcgacgaaa aagccacgta ctttttcacc ggtgaaaatc
      241 cgcaatggga acgggacaaa cccacaggaa tgaagctgtg tgattacctc tatcgaaagg
      301 tggagcggtt cgccatcgag aacggtgtgc aggtggttga tgccgggaag tgctcaccgg
      361 agcagattaa cgcgatgatt gaggtggtca gcgagcggca cggggaaagc gcggtgaacg
      421 agaagctgaa agcggagctg atgcgggaat ataacagccg ctattttaac gtgccggtgc
      481 ctgaactgcc ggaggacaaa cccgcccgcc ggtggtggcc gttcggcaaa aaataacgcc
      541 agcaccggca tggccggtgc tgttattctt ccccgagctt atcccgcatc acctgccaga
      601 gtgtttccgc atcctcgtga agccgttcgc acatgtcggc cggggtatcc atgtccagct
      661 cgttcagctt ttccagcaac agcagcgact gatccttaat gaatttagcc atattgaggg
      721 cagttttttc ctgcttcgtc atgttctcca ttcccttcgg taagttctca gctaaaccag
      781 ccggaccaga agcgtttttt cttctgtgtc agttctgttg tcaatctgtc cacttcggcc
      841 tggagtttcc ggcgctcatc atcgtgctgg cggcgttctt cttcccggga tgttttatct
      901 tccagcatca gggtgacagc ctgccggaga tcggccagtt ccttttgcat tgccagaagc
      961 tgttcctgtg ggaaattttt ttgtgtattt tcacggtgtg aaatgccggg aatttgtttc
     1021 tcagggatat tcacatttcc gtatacacgg atcagttctg acacatccac gacagggttg
     1081 tttttcccgt cacgtgacac ggtaaccttt ccctgtttta catgattgta tagcgttctt
     1141 ctggtaatgc ccgcggcttg tgctgcctgt gaaaggttga gcaatgtttt cgccactgta
     1201 tacccctgat tttatcgggt gtgaaaggtg tgtaatttct cacggtaaat gtatcgctga
     1261 attttacccg tttttccgtt caggatgtgg ttttctgacg cgctgcgctt gtcaaaccgg
     1321 cgacggccac gcaatgaatt gcgcccccgt catcggttat tagaggctgt ctgcccccgg
     1381 atacccggat tctttctgat gcctgacggt gacgatcaca atctggtcca tttcccggtc
     1441 gtggcggtag agcatcacat agccactgtc gccaaacccg atcaccagtt ccttatattc
     1501 cagcggcaga aacggcaccg ggcggccaat gtctggcaat gttttcagtt gctggatagc
     1561 ccggacgatc acctcacctc cttttctggc tgccagccga tttttggttt tgagaaagtc
     1621 ctgaaggcgt tgtaaatcgc gctgtgccag cgcggaaatc gttacctgtg gcatggcgga
     1681 gcatcctgct cgttctcagt tccccaggta cttatccagg cttccgcttc ttccgcagta
     1741 aggtgcagac ccgtttcctg atagtgctgc catgcggcct gtccgtcccg caggtactgg
     1801 ctgcgcttct cttcccggtc gatgtattcg gtgattgcct ccagcattag cgcgtgcgct
     1861 gagcggtgcc ggtcatcggc cagcgttttc agccggtcct taagttcttc atcgagcctg
     1921 atggatgttg ctgatgacat aaaagcccct ttgtagtcat atgtattaca catgactact
     1981 ttaatccgtg gcggcgctat ttgccagaaa tacggctttt gctgcccatt tttcagcttc
     2041 gcttccacta atcagggctg ctacaggctc actcatcatc caggactatt gcagcctgcg
     2101 ggttaaagga cctgttactg gcggcaacca tcatcattga gcttttgggt ggtgactggt
     2161 cgcggatcct gtaagccgtc atttttcaga ctgaattcaa aatttataac ctgtcgcatt
     2221 gccgggtgcc ctgcgggata tagccagtgc ctcattgccg tcagcgtttc ccgaaaaaaa
     2281 aggtgacagc cggtaagcga cacgttgtcc actcgtccat cgtccagaat atcaaacgtg
     2341 gcggtcacct gaccttcgat gcgttttgct gcggcctgta ccgggtaggt tggctgcagt
     2401 gtggttacgg gttttagtgg tatcgcatgt accggagtga ggaacatggc cgccagcact
     2461 gtcagagtaa aagctttcat ggtctgaaat ccagaggggc gtttttgaat atttgccagg
     2521 tattttcatc cggtgccggc ggaattttgg cgtgggtaac ggctgatatt gctgctttac
     2581 agagtttcgg atctcctcct tcagcaatgg cgctgttcag agtgccatca cgttgtatgg
     2641 atatccgcac ggtgcacatt tttcccgcat aggcctctgg ttcaaacatc tgcatttcaa
     2701 cagcggtctt gatggccgca gcgtagctac tgattttaga tgacttatcc agagtataaa
     2761 ttttcggcgg ctgggtggtg gaatgcttac aggccgtcag gagtaagggg ctgcacaatg
     2821 ccagcaggat aaagggtatt aaacgcatcg ggttgatgac ctgtcagtaa aaaatgttac
     2881 atgtatattt gtcactcttt acggccacgt tcatttacat aaacacggct tatcgtatcg
     2941 tttttacacc aaacggcccg ccatgcagtt gcgtgcatag gcatgcagcg ggttacaatt
     3001 taccgggcgc gtttccggcg gttttccggg tggtttgttg cctgttttat cccgtagccg
     3061 ccggaaacgc cctcagaccg tctgagcggt gcgggcaatg cgacgttatg gtaaataccc
     3121 cttaccggcg gcgctgtggc tgccaggtga aggtggtttc cgtagcctcg tatttctccc
     3181 gacgcatttc ctgcgcccgc ctggcttcac gttcgtcctg aatggcacga agcacccttt
     3241 cccgcacctg ttgttttacc gcctttggca gcgttttgtt ccctttcagg cccatggcca
     3301 gtgcctgagc gacaaaggcg tccgccaggc gcttttcttc agcgggagtc agtttcaggt
     3361 gcgtcatagt ggcctccggg cgtctcaggt ttcccgaaca gtttaaccgc aggtcctctc
     3421 ctcatgccag ttagctttca gatgtgtcta taccatctga aatgtaagtg tgatgaatgt
     3481 cttaaataat aaaatttgtt gccatgagaa attttcggga aaagacgcga tctgcttaca
     3541 ggtggctccg ggcgcttttt tgtgcctgaa gatgagaact aatctcatat attgacacct
     3601 gaaaacggga ggagtaaggt aataattata ctgtgtatat gtacagtatg ttggctttaa
     3661 tttattgttt taaatgtcaa agaggaaaac ggtatgagtg gtggagacgg tcgaggtccg
     3721 ggtaattcag gtctgggaca taatggtggt caggccagtg ggaatgtgaa cggtacgtct
     3781 ggtaaaggtg gcccttcatc aggtggtggt acggatccaa acagcgggcc gggctggggt
     3841 acgacgcata cgccgaatgg agatattcat aactataatc cgggggagtt tggtaacggc
     3901 gggagtaaac ccggtggtaa tggtggtaac agcggcaatc atagcggtag ctctggtggc
     3961 ggacagtctt cggccactgc gatggccttc ggtctgcctg ccctggctac tccgggggct
     4021 gaaggactgg ctttatcagt ttccggcgat gcgttgtcgg ccgccgttgc tgatgtgctg
     4081 gctgccctga aggggccgtt taagtttggt ctgtggggga ttgcgatcta cggtgtgctg
     4141 ccttctgaga ttgcaaaaga tgatccgaaa atgatgtcaa aaattatgac gtcattaccg
     4201 gccgatgcgg tgacggagac tccggcaagt actttaccac tggaccaggc gacggttcgt
     4261 gtcagacaac gggttgtgga tgtggtgaag gatgagcggc agcatattgc ggttgtcgca
     4321 ggtcggccaa tgagtgtccc tgtggtggat gcgaaaccga caaaacgtcc gggggtattc
     4381 agtgtgtcga ttccgggtct cccgtctctg caggtgagcg taccgaaagg tgttccgaca
     4441 gcgaaagccc cgccaaaagg cattgttgct gaaaaaggtg attcacgtcc ggctggtttt
     4501 acagccggtg gtaactcccg tgaggccgtt attcgtttcc cgaaagagac cggacagaag
     4561 ccggtttatg tgtcggtgac agatgttctt accccggcac aggtaaaaca gcgtcaggag
     4621 gaagaaaagc gtcgccagca ggcatgggac gccgctcatc cggaagaggg gctgaaaaga
     4681 gactatgata aagcgaaagc tgagctggat gccgaagata aaaatattgc gaccttaaac
     4741 agccgcattg catcgacaga gaaggcgctc cccggtgcaa gggctgctgt tcaggaagcc
     4801 gataaaaagg taaaagaggc agaggcaaat aaagatgatt ttgtgactta taacccgcct
     4861 catgaatatg gctccgggtg gcaggatcag gttcgctatc ttgataagga tattcagaat
     4921 cagaatgaga aattaaaagc ggctcaggca tctttaaacg caatgaatga atccttatcc
     4981 agagataagg ctgcacttac cggggcgatg gagagccgga aacaaaagga gaaaaaagcg
     5041 aaggatgcag aaaataagtt aaatgaggaa aagaacaagc ctcgcaaggg gactaaagac
     5101 tacggccatg attatcatcc agtacctaaa acagaagaca taaagggact tggagagtta
     5161 aagcgaggtg atcctaaaac acctaaacaa ggtggtggtg gtaagcgggc ccgatggtat
     5221 ggcgataaaa aacgtaaaat ttatgaatgg gattcccagc acggtgagct tgaagggtat
     5281 cgtgccagtg atggcgaaca cctaggcgca tttgatccca aaacgggtaa gcaggttaaa
     5341 gggccagatc caaaacggaa cattaaaaaa tatctttgag aggtaaatat ggggcttaaa
     5401 ttacgtttga catggtttga taagaaaact gaagagttta aaggggaaga gtattctaaa
     5461 gactttggtg acgatggttc tgtcattgaa agcttgggaa tgcctttaaa ggataatatt
     5521 aataatggtg agtttgattt gaagaaagaa tgggttcctt tactacagcc attttttaaa
     5581 aatcaaataa aacctgatgt ggataattgt tatatagctt ttgattatcg ggacggtaac
     5641 tggtaacaaa aaattatcaa ggatgatttg aaatactgac ttccggtgtt cagggatgaa
     5701 tgccggagtc tttaaataca gggaaagtat atgaaaaagg taaagactat ttttcttttt
     5761 atattgattg ttgctggctt tcttctggcc gcatgtcagg caaattatat ccgggatgtt
     5821 cagggcggaa cggtggcacc atcgtcctcc tctgaactga cggggatcgc ggttcagtag
     5881 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatcttt tgccctgtaa
     5941 acgaaaaaac cacctgggga ggtggtttga tcgaaggtta agtcagttgg ggaactgctt
     6001 aaccgtggta actggctttc gcagagcaca gcaaccaaat ctgtccttcc agtgtagccg
     6061 gactttggcg cacacttcaa gagcaaccgc gtgtttagct aaacaaatcc tctgcgaact
     6121 cccagttacc aatggctgct gccagtggcg ttttgcgtgc tttcccgggt tggactcaag
     6181 tgaacagtta ccggataagg cgcagcagtc gggctgaacg gggagttctt gcttacagcc
     6241 cagcttggag cgaacgacct acaccgagcc gagataccag tgtgtgagct atgagaaagc
     6301 gccacacttc ccgcaaggga gaacggcgga caggtatccg gtaaacggca gggtcggaac
     6361 aggagagcgc aagagggagc gacctaccgg aaacggtggg gatctttaag tcctgtcggg
     6421 tttcgcccgt actgtcagat tcatggttga gcctcacggc tcccacagat gcaccgaaaa
     6481 agcgtctgtt tatgtgaact ctggcaggag ggcggagcct atggaaaaac gccaccggcg
     6541 cggccctgcc gttttgcctc acatgttagt cccctgctta tccacggaat ctgtgggtaa
     6601 ctttgtatgt gtccgcagcg cccgccgcag tctcacgccc ggagcgtagc gaccgagtga
     6661 gcgaggaggc gcaatttccc cggcacctgc aagacggtca ccggacgcgc tgatgtggga
     6721 tattgtcccc tgattaagtc tttctattcc ctgacgatca attatcagga taggtgaacc
     6781 atgtcggttt ttcgctgaac attttacgtt aacctatctc tgttgctttt ttctccttgc
     6841 ggagaaatgc aagtcatccc cgctggggcg tggctgactt caggggaaaa gaccttgctt
     6901 acgctggctg gacgccaccg acgcacggtc ttttcccctc tttctggctc tgtccggctg
     6961 gacgccggcc acgccagaaa ttcacccggc ccccgtcagg ctcacgcctg accacccaca
     7021 aaaatccacg gtgctggacg cacctgacga agcagcaaag ctgctcctga ttttgcgggt
     7081 ggtcagccgc tcgctatggc gaactctgac gggggccgga acaccggaaa agaccgcact
     7141 ggggtgccgc cttttcctct cacacgctgg ggcgtgctcg ttgttcctcc ggggctgcgc
     7201 cttttccgca tggggcggaa agaggctggc ctccgcggtg aaagctgaac gcaatcacca
     7261 cggacgtgac cgccgccgcc atgccttggg gcatgtccgc ggcttgtgca gcgggggctg
     7321 cgcatcacgc cggtaaaccc gtcgtctgaa ggagagagat tgcatgttta atacggattc
     7381 gcttgcctgg cagtggcata tcccggagct ggcccggcag atttacccgt gggtcagctg
     7441 gctgtaccgg gagtattaca gtctggccgg tttttttctg gttatcggcc tgtttttcgg
     7501 tctggctgaa ctctgggccg gccgaatgcg ttttgtgggg ctgagccata aggcgcggcg
     7561 ggagtatgcc gatcgctata agcgcgataa taaccggact gtgccatttt cggtgctggc
     7621 ctcgctggat tcacccgggc ggatggtcag ggcacggcga catgtctgga actacgcgta
     7681 ctggctgacc ggcgctgccg gtctggccct tctggggggg ctggttgtcg ccgtgtttat
     7741 cgagagctgg ggagaaaccg tcctgcgccc ggacctctat gcgaacgcag attattacct
     7801 gctgcagccg gctaacctgt tcgggatagc ctggtgtgcc ggagggcttg tcgccggcgt
     7861 ccttctggcg cggctgctgg cgttcctgct gcttgatggt cattttgcgg ccgctgacga
     7921 ggcggtgaac gcgaaactga atcaggagag ccggcgcagc agccagcgga ccggagaaat
     7981 gacagacgtc cgccacctgc atttcgggga gccggtaccg gttaacgcgc tggccgattt
     8041 ttccacggag caggcccgga aacagcaggc ggtgtttctg ggtaaggatg agcagggcca
     8101 gccggtgctg gtccctcgtg atacctggcg caagaccaat attcagatcc tcggcctgcc
     8161 cgggagcggc aagagcgcga tggccaccaa cgcgctcatt cgctgcgtac gcgatttcgg
     8221 ggacgctgtg gtgtacttcg accccaaagg ggacgcgtgg gcgccgcacg tcttccgggc
     8281 gcactgcccg gacttcaccc tgctggatct gcggccgggg aaaccggcgc agctgaatct
     8341 gttccgcgat ctcgaccagt acgccctgaa gaatctgctg gttgccgggt ttaacctgag
     8401 tgagaccggc gacgtggcgg accactaccg catcagcgag cagaaagcgg cgaagctgat
     8461 tgcggagcag ttcccgcagg gggcaaacat tcagcaggtg ctggcggcgg cgtacgcgtt
     8521 gccggaggcc ctgaaaaagg acgtgaaggg gctgatcacc aagctggaga acgtggccga
     8581 cctgagcgtc ctgcagacgg acagcggtat cgatgtggcg gggataatca acggcggcgg
     8641 ctgcctgtac gtcatcggct cgatggacga tgaggcggtg atccgggtgc agaagatgct
     8701 gtttgcccgc tgcgcgcaaa tcatcattgc gcgggatgag ttccggaggt ggccgcacgc
     8761 gagcatcatg ctcgatgaga ttaaatatct gctgtcgaag tacgtgctga acgcgctggg
     8821 gacgctgcgc agccgggact gcaacctgct gctcgcgcac cagtcgctgg gcgacttcgg
     8881 tcagtgcggg caggatctgc ccgctgattt tgtgaaaacc acggtactgg ataacacgcc
     8941 gatccgctgg ttctatcgcg cggccagcca ggagtccgcg cagtgggcgg cgggccagac
     9001 cggggagatc cgggtggacg tggagcgccg ccgggccagc cgggaggcgg ggaacgtgga
     9061 gcacatcagc ggcgacagct tcatccagaa ggaggcgcgg ccgctgtttg acgtgaacac
     9121 cctgcaacac ctgcctgacg ggttcgcggt gatgaccggg ctgggcgtgg cgcggctggc
     9181 gttcagcagc ccgctgcggg ttgagcgccg ggaaatttcg ctgaaaacct tcccggtgct
     9241 ggcgaaaacg gacccgctgg cggagtacct gcctccggcc gacggcggcg gaccaaaagc
     9301 ggatgatgat tttgcggggc tttactgatg acactggagc agtacaacgt cagccacgcg
     9361 aagcggcagg cgcggaacgc ggagaaaaca cggctgaccc tgcgctggct gcgggaagag
     9421 ctgtgttcca cggcggagct ggttgcgcga cggctgggga ttgcggcggt acagccggtt
     9481 taccgcttcc tggacagtct ggtggcgaaa gggctgctgg tccgggcgaa atacccggtt
     9541 gacggccgtc aggtcagcgt gtgggggctg acgccgcacg gggtggcgtt ctcgttcgac
     9601 gaggacgagc cgctgacgga cgttattccg tttcagccgt caagggtgtc ggcggcgcag
     9661 ctgccgcacc ggctggccgt gcagtccctg cgcctctcca tggagggccg gggggccacc
     9721 ggctggcggt acctgcaccg tatggccctg aaagggatga aagtgccgga cgcgctggcg
     9781 gagctggacg gcagaacggt ggcatttgag gtagagcgca ccgtcaagag ccgcaggcgg
     9841 tatcaggaag tggtgtccgg gtatctgttc aaccgccggg cgaacggcat cgatgaaatc
     9901 tggtacatct gccctgaccg gccgacgctt gtccgggtac agcgggcgat tctggcggtg
     9961 gatgaaatcg tgaatccaca gaccggcgag gcccgcaaaa cggtggagct ggaccgggag
    10021 aggctgttcg cgtgctttaa atttatgaca acggagtgag
//
