LOCUS       NZ_CP025460             5596 bp    DNA     circular CON 25-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP69 plasmid p69-4, complete sequence.
ACCESSION   NZ_CP025460
VERSION     NZ_CP025460.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08180551
            Assembly: GCF_002848545.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Tang,Y., Fu,P., Tian,Y., Zhang,H. and Jiang,X.
  TITLE     The role of Crispr in the dissemination of blaKPC in klebsiella
            pneumoneae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Tang,Y., Fu,P., Tian,Y., Zhang,H. and Jiang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-2017) Huashan Hospital, Shanghai Medical College,
            Fudan University, Department of Laboratory Medicine, No. 12, Urumqi
            Middle Road, Jing 'An District, Shanghai, Shanghai 200040, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-OCT-2017
            Assembly Method :: A5-miseq v. v20150522; CANU v. v1.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 209.0x
            Sequencing Technology             :: Illumina MiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/25/2023 03:54:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,762
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 72 of 171
            Pseudo Genes (incomplete)         :: 106 of 171
            Pseudo Genes (internal stop)      :: 20 of 171
            Pseudo Genes (multiple problems)  :: 24 of 171
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP025460.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP69"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p69-4"
                     /geo_loc_name="China:Shanghai"
                     /collection_date="2017-05-17"
     gene            632..790
                     /locus_tag="CYD98_RS31820"
     CDS             632..790
                     /locus_tag="CYD98_RS31820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1148..1474
                     /locus_tag="CYD98_RS30340"
                     /old_locus_tag="CYD98_30330"
     CDS             1148..1474
                     /locus_tag="CYD98_RS30340"
                     /old_locus_tag="CYD98_30330"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            1500..2045
                     /locus_tag="CYD98_RS30345"
                     /old_locus_tag="CYD98_30335"
     CDS             1500..2045
                     /locus_tag="CYD98_RS30345"
                     /old_locus_tag="CYD98_30335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            2111..2461
                     /locus_tag="CYD98_RS30350"
                     /old_locus_tag="CYD98_30340"
     CDS             2111..2461
                     /locus_tag="CYD98_RS30350"
                     /old_locus_tag="CYD98_30340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2531..2797)
                     /locus_tag="CYD98_RS30355"
                     /old_locus_tag="CYD98_30345"
     CDS             complement(2531..2797)
                     /locus_tag="CYD98_RS30355"
                     /old_locus_tag="CYD98_30345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2781..3005)
                     /locus_tag="CYD98_RS30360"
                     /old_locus_tag="CYD98_30350"
     CDS             complement(2781..3005)
                     /locus_tag="CYD98_RS30360"
                     /old_locus_tag="CYD98_30350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(3672..3884)
                     /locus_tag="CYD98_RS30365"
                     /old_locus_tag="CYD98_30355"
     CDS             complement(3672..3884)
                     /locus_tag="CYD98_RS30365"
                     /old_locus_tag="CYD98_30355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3891..4400)
                     /locus_tag="CYD98_RS30370"
                     /old_locus_tag="CYD98_30360"
     CDS             complement(3891..4400)
                     /locus_tag="CYD98_RS30370"
                     /old_locus_tag="CYD98_30360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4435..5139)
                     /locus_tag="CYD98_RS31825"
                     /old_locus_tag="CYD98_30365"
                     /pseudo=""
     CDS             complement(<4435..5139)
                     /locus_tag="CYD98_RS31825"
                     /old_locus_tag="CYD98_30365"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5078..5422)
                     /locus_tag="CYD98_RS30380"
                     /old_locus_tag="CYD98_30370"
     CDS             complement(5078..5422)
                     /locus_tag="CYD98_RS30380"
                     /old_locus_tag="CYD98_30370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5412..5588)
                     /locus_tag="CYD98_RS31260"
     CDS             complement(5412..5588)
                     /locus_tag="CYD98_RS31260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     Typing          complement(79..155)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          344..741
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(3557..5088)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttcgcaaac gccggacggc acggaaaagt tttccgggtc gctccggcgt tggacaagcg
       61 ccagcgcgtc agtggtgtta gcgggtttcg gggcgcagcc ctgaaccagt cacagcgttt
      121 acgctgtgta tactggctta ctatgttggc atgagagcac gattcatgaa agtgcctcat
      181 gtggcaggtg tgatatgtgg ctcaggagcg tccgcaatac catgcacgga cagatgaaat
      241 gacgcctcct cgctcactcg gtagctgcgc tcctgccgtt cggctgcggc gggcgtgacc
      301 gactcacgga taacgggata aagcaaaggc gctcaaatca gaaagggccg gagaaaagga
      361 tgcggcgttg ccgtttttcc ataggctccg cctcccctga cgagcatcac agaaatggac
      421 gctcaagtca gaggaggacg aaacccgaca ggacttaaag atgccggtga tttctctccg
      481 gcggctccct cgtgcgctct cctgttccga ccctgccgtt taccggatac ctgttccgcc
      541 tttttccctt tcgggaagcg tggcgctttc tcatagctca cgctgctggt atctcggctc
      601 ggtttaggtc gttcgctcca agctgggctg tatgcacgaa ccccccgttc agcccgactg
      661 ctgcgccctt tccggtaact atcatcttga gtccaacccg gacggacacg caaaaacgcc
      721 actggcagca gccattggta actggatata tacgagagag atattgagag gtcttgaagt
      781 gggtgcctaa ctgcggctac actgaaagaa cagttttggt atctgcggtc tcgtataccc
      841 agttacccgg ttaagcagtt ccccaactga cttaaccttc gatcaaaccg cctccccagg
      901 cggttttttc gtttacagtg caagagatta cgcgcagaaa aaaaggatct caagaagatc
      961 ctttaaatga tcgtcagttc tgttatcccc ggagctccat ccatcccacg gaaaaacacc
     1021 cgccactgat gccctcagcg cactgcacat catgtacaaa ccactccctc cctccttttt
     1081 tattgcgttt cttaatgcgc aatggtagct ttgtgcgcaa caaaaaacgc aatagcaagg
     1141 gggggtaatg agagaaagaa caaatatcat gctagatata ggattaaagc aatccttaca
     1201 acggctttcg aataaaacca aaatgagtct aagtgatctt attaaccaga gattatcagg
     1261 ttcactttca gttgaagaac gcattggcga aaagcctgaa tggatgagaa accttgatcc
     1321 ctatctcgcc aatcactttt caaatattga gttaccgctc ccttcaccat taaaagaata
     1381 ccaagaaaaa atggatgaga tagattcaat tattaatgac atgatggttc taaaggaata
     1441 tttcagagct aagttttacg acgaaataaa ataggcacgc tatacggagg cggagaatta
     1501 tgttttttaa acggtattta acggagaagg aaatatacaa ggcagcactc ccaattgtta
     1561 ggcttcaagg gagagttaac acttcattac gttttttatc caaatactca gatgtaaaca
     1621 aaaatggatt aattgcattg catcttaatt tattacattt tatctcatac aacatacacg
     1681 gtgtagagtc tctgaaaatc actcaagcag gcataaacaa agctgtacaa caaagccctc
     1741 acgcagatga aataaaaaat attgcgcagg caataggacg caaaggacct gagtacatcg
     1801 aaggattaat tagaaaccca tgggggaaga aaaatgataa tccactaata tttataatga
     1861 gcattgaaga aagtataaaa gaaaccctta actcaggtta tatgttatat agagtagctc
     1921 ccataactga gggtgaagca gctgctttca tggatgttct taaacaatta ttattatttg
     1981 aggataattt tcgccttttt ggtagagact tggtttataa aatagaaaaa ttaaaaatca
     2041 gataacaaag tatggtgaac gaaaaatgaa agagaggata gtgaaatgtg taataccaag
     2101 attctcaact atgtattggt taattgcgtt cctagcattt atattccccg gtcgatcttt
     2161 tgctgacggt tacaccgctt actaccgatg taatggtact caggaagcat ctcttactgt
     2221 taaagataac aaactaagta tggttgtagg tctttctaag tttaataggc aactggatac
     2281 ttatagattc aaagaaaagg gaatcgaagt agtaatgcag agagctgctt ctgatgataa
     2341 ctctgaggcc ataatgtctt ttattatgcc tgacgttaac aatatgacct tattagttaa
     2401 aaataacggc tacattatcg ttaagacaac ctgctggtta actaaaaccg acaaacagta
     2461 gaacacgtag ttagtcgagt atttaccata atacctgttg tcggcatcaa aaaagcccgc
     2521 atagcgggct tcagtcgtaa acttcgcggc gatgtccaat cgtggcggca aggataacaa
     2581 gctcgtcatc tcggatctca cacagaacgc gataatctcc cacccgatag cgccagaact
     2641 cgcccagctc acctttgagc ggcttccctg actggcgagg atcggcagca actgcaatgc
     2701 gtaaactcat aaaatccaca atccgtcgtg cgttctgttt gtccatcttg cgtaacgatt
     2761 tgagcgcccg atcggaatag ttaatcgtcc agaccaagtg acttcctcac gtcttcagac
     2821 gaatgcactg cttcatcacc acggcgaacg cgtgcagcag tttctgctga aagataataa
     2881 tcctccaggt cttccagatg cgccagtatt gcttcccgcg cataaaacgt ctttgttctg
     2941 ccggtttgct tcgccagcga gtccagacgg gactcaatct catcagataa ccggatagca
     3001 agcatataaa cctcccgaac accatttgct atacatgtat atcaacagta ccacaatcaa
     3061 cctaaaagaa caaaaaataa tcttgttagc ccctcttgat aatccgtaac gcggtcaata
     3121 tatctagctt gagggctttc ctcttaacta ttgaaaggat aacgattatg ggtcttaagc
     3181 cgggtccgaa acgaattgcc gattcaacag gggaacctga taaacgccag cgtgacaata
     3241 aaaaaacgcc gggaaatact gacaaattga aacccagcaa atcttcaaaa aaataagtca
     3301 gtagttaaaa gccctccggg gcttttttgt atctgccgtt cacgctgatt aaacataaca
     3361 cccgttgccc gcaccgcccc ggacgggttc gccgcgaacg gggtggcagt caggtaaaac
     3421 cggcaacaaa ccacccgata aaccgccccg ttcgcgacag ataaatttta cctggctgca
     3481 tagccatgca gccaggtgaa tgacatccgg tttcagcgta aatgcccgat gagaaccctg
     3541 ttttcctgca tttcagccca gcgacggacc gtcatagctt cgctgccggg aacgcgcttc
     3601 cgtcagttcc ttttcgtgct gccgttgctc gtgcgcccgt tctgcggcca cggccatctc
     3661 atgacgctgc actaccggct caaacgctgc agttgcccgc tcaagcgttc cacctgctga
     3721 ctcaagcgcg tcacgcgctc gctcagcgcc gcgttctccc gttgcataag ccctgacatt
     3781 ttccgccatt ccccgaaggc gctctcccac tcgtccagcc ttttcgagta gtcctgctgt
     3841 agctgctcta atgcgctcag caactgtttt tccagctcgg tcatcggcac ttactccttc
     3901 accagtctca gcgcgttctg cttcgaccgc ccgttcccga ccgtccagct ctgcgtcctg
     3961 tctacggccg tccccttcgg cagcaccaga aaacgtccgt tgctgtcttc caggtacgtc
     4021 acgccccagg tcttcgcgtt cagcttcgac agcgctgcgt tctgccggtc gatttcggcc
     4081 agatttgacg ctatcaagct cccctgatac cacaacaccc cgctcaggct ggcgaacagc
     4141 agtcctgaca ccatcgtgat ggtcagccat gtccgcccga ccatacgcat cacgctcagg
     4201 cggttcgact gcatggccgc gctcataccc tgctcgtggg cgcggatggc ggcgctgatt
     4261 ctcttcccgc tttcgctcag ttccgctctg acagacttct cgtgctcgct gaatgcggct
     4321 ttcagcattt cgccggtact ctgctgctgc gctttcgatt tctgctctaa gtccttcgcc
     4381 agcgttaaaa gactgttcat agatggctcc cttaagtcgg atgtttcgcc ctccgtccgg
     4441 gtcggcaatg ctgatgctgc tctttgtggt gcgcacgacc tcaaaacctg cgctttccag
     4501 cgcctcagtg acgtcctgac gcgttttaag ctcccctgac gaggcgaggg ccagtaagcc
     4561 ccgcgtaata gcctctgcgg cttcctgctt cgttttgggt aacgaagatg gcgtcaccag
     4621 cgcgcgccgg ttctccggtg cgttcgggtc gtgcagcccc agtcgcccgt tcaccacggt
     4681 ctgccacgca tcgatgcgcg gacggtctgc ccggtcgtag tacggctgga gacgcctgcc
     4741 ggtcagcagt tccgtgttcg gtaccagaaa attcagttcc agccgcccct tgtcccggtg
     4801 ttcaacccac agcacgctgt actggtcttt atcgagtccg ggcatcagaa cccgctcgaa
     4861 gctcgccatc agcttttcac gctggccggg cggtaaatcc tgttcagcaa aagacaggac
     4921 gccggaggtg tactttttgg cgtagggcga ggcgtcgata agctcccgga cctcctccgg
     4981 ctttccctgc aggacgatgg ccccgtcgcg ctggcggtct ttccccagca gataatccac
     5041 cggcccggca ccgccgcctc gccctcgcgg atgaaactta acgatcatcg tctgccccct
     5101 tttccagcac ggcatgccgc agccgctcga gttcggcatc aatggccatc agcgcggcca
     5161 ccacctgaac ccggtcagcg cctgaccact ggccgccgtt aattttccgg gcgatctggt
     5221 tgaggttgtt ccccatcccg gcaagctggc gcagcaaaac cggatcgatg gacggcagcc
     5281 gcaacgaccg cgccgggcgc gtatcgagac aggtttcccg catccacacc gccagttgcc
     5341 ttccattgca gcgctccagc agctgctggt actccccatc ggtcacccgg atacctaaaa
     5401 aacgggtacg cttaaccgcc atcctctcct ccgatatccg caaaatgtga ccgggctgaa
     5461 cgctcaagct ctgccatctg ctcccggcgg taatcttcct cctgtgcctg acgcatcaga
     5521 tttgccatca ctgccggatg ccaggatctt ttttttacga cgagcggctt cttcttcggc
     5581 ttctgcatta tccctc
//
