LOCUS       NZ_CP025338            11080 bp    DNA     circular CON 19-MAR-2024
DEFINITION  Salmonella enterica subsp. enterica serovar Typhimurium strain BL10
            plasmid p11k, complete sequence.
ACCESSION   NZ_CP025338
VERSION     NZ_CP025338.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07945789
            Assembly: GCF_002762135.3
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Typhimurium (Salmonella
            typhimurium)
  ORGANISM  Salmonella enterica subsp. enterica serovar Typhimurium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 11080)
  AUTHORS   Hong,Y.P., Wang,Y.W., Huang,I.H., Liao,Y.C., Kuo,H.C., Liu,Y.Y.,
            Tu,Y.H., Chen,B.H., Liao,Y.S. and Chiou,C.S.
  TITLE     Genetic Relationships among Multidrug-Resistant Salmonella enterica
            Serovar Typhimurium Strains from Humans and Animals
  JOURNAL   Antimicrob Agents Chemother 62 (5), e00213-18 (2018)
   PUBMED   29581119
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11080)
  AUTHORS   Hong,Y.P., Chiou,C.S. and Wang,Y.W.
  TITLE     Salmonella enterica subsp. enterica serovar Typhimurium strain BL10
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 11080)
  AUTHORS   Hong,Y.P., Chiou,C.S. and Wang,Y.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2018) Center for Diagnostics and Vaccine
            Development, Centers for Disease Control, R.O.C (Taiwan), No.20,
            Wenxin S. 3rd Rd., Nantun Dist., Taichung 408, Taiwan
  REMARK    Sequence update by submitter
REFERENCE   4  (bases 1 to 11080)
  AUTHORS   Hong,Y.P., Chiou,C.S. and Wang,Y.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2017) Center for Diagnostics and Vaccine
            Development, Centers for Disease Control, R.O.C (Taiwan), No.20,
            Wenxin S. 3rd Rd., Nantun Dist., Taichung 408, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUL-2017
            Assembly Method                   :: CLC NGS Cell v. 10.1.1
            Genome Representation             :: Partial
            Expected Final Version            :: No
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002762135.3-RS_2024_03_19
            Annotation Date                   :: 03/19/2024 06:42:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,079
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,827
            CDSs (with protein)               :: 4,827
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 38 of 130
            Pseudo Genes (incomplete)         :: 96 of 130
            Pseudo Genes (internal stop)      :: 27 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            Pseudo Genes (short protein)      :: 1 of 130
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP025338.2.
            On Jul 15, 2018 this sequence version replaced NZ_CP025338.1.
            Bacteria available from necropsy of sick pigs.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11080
                     /organism="Salmonella enterica subsp. enterica serovar
                     Typhimurium"
                     /mol_type="genomic DNA"
                     /strain="BL10"
                     /serovar="Typhimurium"
                     /isolation_source="sick pig"
                     /host="Sus scrofa domesticus"
                     /sub_species="enterica"
                     /db_xref="taxon:90371"
                     /plasmid="p11k"
                     /geo_loc_name="Taiwan"
                     /lat_lon="23.29 N 120.27 E"
                     /collection_date="2011"
                     /collected_by="Hung-Chih Kuo"
     gene            complement(join(10685..11080,1..45))
                     /locus_tag="CTI29_RS24870"
                     /old_locus_tag="CTI29_25100"
     CDS             complement(join(10685..11080,1..45))
                     /locus_tag="CTI29_RS24870"
                     /old_locus_tag="CTI29_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166363.1"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_025732385.1"
                     /translation="MSSNGRHFGEIRLSALKKAPEMFGSVYEHEEKKPMEAFAERLRDA
                     VAFGAYIDGEIIGLSVFKQEDGPKDAHKAHLSGVFVEPEQRGRGVAGMLLRAVIDYGRE
                     HVEQIMLTVAEGNEAAINLYQRSARCPAPLQSPRRSAGRQSK"
                     /name="GNAT family N-acetyltransferase"
     gene            complement(504..788)
                     /locus_tag="CTI29_RS24875"
                     /old_locus_tag="CTI29_25105"
     CDS             complement(504..788)
                     /locus_tag="CTI29_RS24875"
                     /old_locus_tag="CTI29_25105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            1092..>1580
                     /locus_tag="CTI29_RS27435"
                     /pseudo=""
     CDS             1092..>1580
                     /locus_tag="CTI29_RS27435"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            1735..2148
                     /locus_tag="CTI29_RS27440"
     CDS             1735..2148
                     /locus_tag="CTI29_RS27440"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            2247..3116
                     /locus_tag="CTI29_RS27445"
     CDS             2247..3116
                     /locus_tag="CTI29_RS27445"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_229382459.1"
                     /translation="MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDL
                     SEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASA
                     DSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQ
                     EKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRS
                     AEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMD
                     RGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            3177..3389
                     /locus_tag="CTI29_RS24885"
                     /old_locus_tag="CTI29_25115"
     CDS             3177..3389
                     /locus_tag="CTI29_RS24885"
                     /old_locus_tag="CTI29_25115"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            3391..3597
                     /gene="urfF"
                     /locus_tag="CTI29_RS24890"
                     /old_locus_tag="CTI29_25120"
     CDS             3391..3597
                     /gene="urfF"
                     /locus_tag="CTI29_RS24890"
                     /old_locus_tag="CTI29_25120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     gene            3627..4466
                     /locus_tag="CTI29_RS24895"
                     /old_locus_tag="CTI29_25125"
     CDS             3627..4466
                     /locus_tag="CTI29_RS24895"
                     /old_locus_tag="CTI29_25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            4453..5304
                     /gene="repC"
                     /locus_tag="CTI29_RS24900"
                     /old_locus_tag="CTI29_25130"
     CDS             4453..5304
                     /gene="repC"
                     /locus_tag="CTI29_RS24900"
                     /old_locus_tag="CTI29_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            5613..6428
                     /gene="sul2"
                     /locus_tag="CTI29_RS24915"
                     /old_locus_tag="CTI29_25145"
     ARG             5614..6425
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             6874..7292
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             6490..7289
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            7292..8128
                     /locus_tag="CTI29_RS24925"
                     /old_locus_tag="CTI29_25155"
     CDS             7292..8128
                     /locus_tag="CTI29_RS24925"
                     /old_locus_tag="CTI29_25155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            8515..8757
                     /locus_tag="CTI29_RS24940"
                     /old_locus_tag="CTI29_25170"
     CDS             8515..8757
                     /locus_tag="CTI29_RS24940"
                     /old_locus_tag="CTI29_25170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(8799..9449)
                     /gene="tetR(A)"
                     /locus_tag="CTI29_RS24945"
                     /old_locus_tag="CTI29_25175"
     CDS             complement(8799..9449)
                     /gene="tetR(A)"
                     /locus_tag="CTI29_RS24945"
                     /old_locus_tag="CTI29_25175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_012850383.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHSGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            9555..10754
                     /gene="tet(A)"
                     /locus_tag="CTI29_RS24950"
                     /old_locus_tag="CTI29_25180"
     ARG             9556..10751
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="99.75"
                     /coverage="100.0"
     ARG             7293..8125
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          743..898
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          3645..4466
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          988..3113
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggccgacaat cggatctcgc cgaaatgcct gccgttgcta gacattgcca gccagtgccc
       61 gtcactcccg tactaactgt cacgaacccc tgcaataact gtcacgcccc cctgcaataa
      121 ctgtcacgaa cccctgcaat aactgtcacg cccccaaacc tgcaaaccca gcaggggcgg
      181 gggctggcgg ggtgttggaa aaatccatcc atgattatct aagaataatc cactaggcgc
      241 ggttatcagc gcccttgtgg ggcgctgctg cccttgccca atatgcccgg ccagaggccg
      301 gatagctggt ctattcgctg cgctaggcta cacaccgccc caccgctgcg cggcaggggg
      361 aaaggcgggc aaagcccgct aaaccccaca ccaaaccccg cagaaatacg ctggagcgct
      421 tttagccgct ttagcggcct ttccccctac ccgaagggtg ggggcgcgtg tgcagccccg
      481 cagggcctgt ctcggtcgat cattcagccc ggctcatcct tctggcgtgg cggcagaccg
      541 aacaaggcgc ggtcgtggtc gcgttcaagg tacgcatcca ttgccgccat gagccgatcc
      601 tccggccact cgctgctgtt caccttggcc aaaatcatgg cccccaccag caccttgcgc
      661 cttgtttcgt tcttgcgctc ttgctgctgt tcccttgccc gcacccgctg aatttcggca
      721 ttgattcgcg ctcgttgttc ttcgagcttg gccagccgat ccgccgcctt gttgctcccc
      781 ttaaccatct tgacacccca ttgttaatgt gctgtctcgt aggctatcat ggaggcacag
      841 cggcggcaat cccgacccta ctttgtaggg gagggcgcac ttaccggttt ctcttcgaga
      901 aactggccta acggccaccc ttcgggcggt gcgctctccg agggccattg catggagccg
      961 aaaagcaaaa gcaacagcga ggcagcatgg cgatttatca ccttacggcg aaaaccggca
     1021 gcaggtcggg cggccaatcg gccagggcca aggccgacta catccagcgc gaaggcaagt
     1081 atgcccgcga catggatgaa gtcttgcacg ccgaatccgg gcacatgccg gagttcgtcg
     1141 agcggcccgc cgactactgg gatgctgccg acctgtatga acgcgccaat gggcggctgt
     1201 tcaaggaggt cgaatttgcc ctgccggtcg agctgaccct cgaccagcag aaggcgctgg
     1261 cgtccgagtt cgcccagcac ctgactggtg ccgagcgcct gccgtatacg ctggccatcc
     1321 atgccggtgg cggcgagaac ccgcactgcc acctgatgat ctccgagcgg atcaatgacg
     1381 gcatcgagcg gcccgccgct cagtggttca agcggtacaa cggcaagacc ccggagaagg
     1441 gcggggcaca gaagaccgaa gcgctcaagc ccaaggcatg gcttgagcag acccgcgagg
     1501 catgggccga ccatgccaac cgggcattag agcgggctgg ccacgacgcc cgcattgacc
     1561 acagaacact tgaggcgcag ggcatcgagc gcctgcccgg tgttcacctg gggccgaacg
     1621 tggtggagat ggaaggccgg ggcatccgca ccgaccgggc agacgtggcc ctgaacatcg
     1681 acaccgccaa cgcccagatc atcgacttac aggaataccg ggaggcaata gaccatgaac
     1741 gcaatcgaca gagtgaagaa atccagaggc atcaacgagt tagcggagca gatcgaaccg
     1801 ctggcccaga gcatggcgac actggccgac gaagcccggc aggtcatgag ccagacccag
     1861 caggccagcg aggcgcaggc ggcggagtgg ctgaaagccc agcgccagac aggggcggca
     1921 tgggtggagc tggccaaaga gttgcgggag gtagccgccg aggtgagcag cgccgcgcag
     1981 agcgcccgga gcgcgtcgcg ggggtggcac tggaagctat ggctaaccgt gatgctggct
     2041 tccatgatgc ctacggtggt gctgctgatc gcatcgttgc tcttgctcga cctgacgcca
     2101 ctgacaaccg aggacggctc gatctggctg cgcttggtgg cccgatgaag aacgacagga
     2161 ctttgcaggc cataggccga cagctcaagg ccatgggctg tgagcgcttc gatatcggcg
     2221 tcagggacgc caccaccggc cagatgatga accgggaatg gtcagccgcc gaagtgctcc
     2281 agaacacgcc atggctcaag cggatgaatg cccagggcaa tgacgtgtat atcaggcccg
     2341 ccgagcagga gcggcatggt ctggtgctgg tggacgacct cagcgagttt gacctggatg
     2401 acatgaaagc cgagggccgg gagcctgccc tggtagtgga aaccagcccg aagaactatc
     2461 aggcatgggt caaggtggcc gacgccgcag gcggtgaact tcgggggcag attgcccgga
     2521 cgctggccag cgagtacgac gccgacccgg ccagcgccga cagccgccac tatggccgct
     2581 tggcgggctt caccaaccgc aaggacaagc acaccacccg cgccggttat cagccgtggg
     2641 tgctgctgcg tgaatccaag ggcaagaccg ccaccgctgg cccggcgctg gtgcagcagg
     2701 ctggccagca gatcgagcag gcccagcggc agcaggagaa ggcccgcagg ctggccagcc
     2761 tcgaactgcc cgagcggcag cttagccgcc accggcgcac ggcgctggac gagtaccgca
     2821 gcgagatggc cgggctggtc aagcgcttcg gtgatgacct cagcaagtgc gactttatcg
     2881 ccgcgcagaa gctggccagc cggggccgca gtgccgagga aatcggcaag gccatggccg
     2941 aggccagccc agcgctggca gagcgcaagc ccggccacga agcggattac atcgagcgca
     3001 ccgtcagcaa ggtcatgggt ctgcccagcg tccagcttgc gcgggccgag ctggcacggg
     3061 caccggcacc ccgccagcga ggcatggaca ggggcgggcc agatttcagc atgtagtgct
     3121 tgcgttggta ctcacgcctg ttatactatg agtactcacg cacagaaggg ggttttatgg
     3181 aatacgaaaa aagcgcttca gggtcggtct acctgatcaa aagtgacaag ggctattggt
     3241 tgcccggtgg ctttggttat acgtcaaaca aggccgaggc tggccgcttt tcagtcgctg
     3301 atatggccag ccttaacctt gacggctgca ccttgtcctt gttccgcgaa gacaagcctt
     3361 tcggccccgg caagtttctc ggtgactgat atgaaagacc aaaaggacaa gcagaccggc
     3421 gacctgctgg ccagccctga cgctgtacgc caagcgcgat atgccgagcg catgaaggcc
     3481 aaagggatgc gtcagcgcaa gttctggctg accgacgacg aatacgaggc gctgcgcgag
     3541 tgcctggaag aactcagagc ggcgcagggc gggggtagtg accccgccag cgcctaacca
     3601 ccaactgcct gcaaaggagg caatcaatgg ctacccataa gcctatcaat attctggagg
     3661 cgttcgcagc agcgccgcca ccgctggact acgttttgcc caacatggtg gccggtacgg
     3721 tcggggcgct ggtgtcgccc ggtggtgccg gtaaatccat gctggccctg caactggccg
     3781 cacagattgc aggcgggccg gatctgctgg aggtgggcga actgcccacc ggcccggtga
     3841 tctacctgcc cgccgaagac ccgcccaccg ccattcatca ccgcctgcac gcccttgggg
     3901 cgcacctcag cgccgaggaa cggcaagccg tggctgacgg cctgctgatc cagccgctga
     3961 tcggcagcct gcccaacatc atggccccgg agtggttcga cggcctcaag cgcgccgccg
     4021 agggccgccg cctgatggtg ctggacacgc tgcgccggtt ccacatcgag gaagaaaacg
     4081 ccagcggccc catggcccag gtcatcggtc gcatggaggc catcgccgcc gataccgggt
     4141 gctctatcgt gttcctgcac catgccagca agggcgcggc catgatgggc gcaggcgacc
     4201 agcagcaggc cagccggggc agctcggtac tggtcgataa catccgctgg cagtcctacc
     4261 tgtcgagcat gaccagcgcc gaggccgagg aatggggtgt ggacgacgac cagcgccggt
     4321 tcttcgtccg cttcggtgtg agcaaggcca actatggcgc accgttcgct gatcggtggt
     4381 tcaggcggca tgacggcggg gtgctcaagc ccgccgtgct ggagaggcag cgcaagagca
     4441 agggggtgcc ccgtggtgaa gcctaagaac aagcacagcc tcagccacgt ccggcacgac
     4501 ccggcgcact gtctggcccc cggcctgttc cgtgccctca agcggggcga gcgcaagcgc
     4561 agcaagctgg acgtgacgta tgactacggc gacggcaagc ggatcgagtt cagcggcccg
     4621 gagccgctgg gcgctgatga tctgcgcatc ctgcaagggc tggtggccat ggctgggcct
     4681 aatggcctag tgcttggccc ggaacccaag accgaaggcg gacggcagct ccggctgttc
     4741 ctggaaccca agtgggaggc cgtcaccgct gatgccatgg tggtcaaagg tagctatcgg
     4801 gcgctggcaa aggaaatcgg ggcagaggtc gatagtggtg gggcgctcaa gcacatacag
     4861 gactgcatcg agcgcctttg gaaggtatcc atcatcgccc agaatggccg caagcggcag
     4921 gggtttcggc tgctgtcgga gtacgccagc gacgaggcgg acgggcgcct gtacgtggcc
     4981 ctgaacccct tgatcgcgca ggccgtcatg ggtggcggcc agcatgtgcg catcagcatg
     5041 gacgaggtgc gggcgctgga cagcgaaacc gcccgcctgc tgcaccagcg gctgtgtggc
     5101 tggatcgacc ccggcaaaac cggcaaggct tccatagata ccttgtgcgg ctatgtctgg
     5161 ccgtcagagg ccagtggttc gaccatgcgc aagcgccgcc agcgggtgcg cgaggcgttg
     5221 ccggagctgg tcgcgctggg ctggacggta accgagttcg cggcgggcaa gtacgacatc
     5281 acccggccca aggcggcagg ctgacccccc cccactctat tgtaaacaag acatttttat
     5341 cttttatatt caatggctta ttttcctgct aattggtaat accatgaaaa ataccatgct
     5401 cagaaaaggc ttaacaatat tttgaaaaat tgcctactga gcgctgccgc acagctccat
     5461 aggccgcttt cctggctttg cttccagatg tatgctcttc tgctcctgca gctaatggat
     5521 caccgcaaac aggttactcg cctggggatt ccctttcgac ccgagcatcc gtatgatact
     5581 catgctcgat tattattatt atagaagccc ccatgaataa atcgctcatc attttcggca
     5641 tcgtcaacat aacctcggac agtttctccg atggaggccg gtatctggcg ccagacgcag
     5701 ccattgcgca ggcgcgtaag ctgatggccg agggggcaga tgtgatcgac ctcggtccgg
     5761 catccagcaa tcccgacgcc gcgcctgttt cgtccgacac agaaatcgcg cgtatcgcgc
     5821 cggtgctgga cgcgctcaag gcagatggca ttcccgtctc gctcgacagt tatcaacccg
     5881 cgacgcaagc ctatgccttg tcgcgtggtg tggcctatct caatgatatt cgcggttttc
     5941 cagacgctgc gttctatccg caattggcga aatcatctgc caaactcgtc gttatgcatt
     6001 cggtgcaaga cgggcaggca gatcggcgcg aggcacccgc tggcgacatc atggatcaca
     6061 ttgcggcgtt ctttgacgcg cgcatcgcgg cgctgacggg tgccggtatc aaacgcaacc
     6121 gccttgtcct tgatcccggc atggggtttt ttctgggggc tgctcccgaa acctcgctct
     6181 cggtgctggc gcggttcgat gaattgcggc tgcgcttcga tttgccggtg cttctgtctg
     6241 tttcgcgcaa atcctttctg cgcgcgctca caggccgtgg tccgggggat gtcggggccg
     6301 cgacactcgc tgcagagctt gccgccgccg caggtggagc tgacttcatc cgcacacacg
     6361 agccgcgccc cttgcgcgac gggctggcgg tattggcggc gctgaaagaa accgcaagaa
     6421 ttcgttaact gcacattcgg gatatttctc tatattcgcg cttcatcaga aaactgaagg
     6481 aacctccatt gaatcgaact aatatttttt ttggtgaatc gcattctgac tggttgcctg
     6541 tcagaggcgg agaatctggt gattttgttt ttcgacgtgg tgacgggcat gccttcgcga
     6601 aaatcgcacc tgcttcccgc cgcggtgagc tcgctggaga gcgtgaccgc ctcatttggc
     6661 tcaaaggtcg aggtgtggct tgccccgagg tcatcaactg gcaggaggaa caggagggtg
     6721 catgcttggt gataacggca attccgggag taccggcggc tgatctgtct ggagcggatt
     6781 tgctcaaagc gtggccgtca atggggcagc aacttggcgc tgttcacagc ctatcggttg
     6841 atcaatgtcc gtttgagcgc aggctgtcgc gaatgttcgg acgcgccgtt gatgtggtgt
     6901 cccgcaatgc cgtcaatccc gacttcttac cggacgagga caagagtacg ccgcagctcg
     6961 atcttttggc tcgtgtcgaa cgagagctac cggtgcggct cgaccaagag cgcaccgata
     7021 tggttgtttg ccatggtgat ccctgcatgc cgaacttcat ggtggaccct aaaactcttc
     7081 aatgcacggg tctgatcgac cttgggcggc tcggaacagc agatcgctat gccgatttgg
     7141 cactcatgat tgctaacgcc gaagagaact gggcagcgcc agatgaagca gagcgcgcct
     7201 tcgctgtcct attcaatgta ttggggatcg aagcccccga ccgcgaacgc cttgccttct
     7261 atctgcgatt ggaccctctg acttggggtt gatgttcatg ccgcctgttt ttcctgctca
     7321 ttggcacgtt tcgcaacctg ttctcattgc ggacaccttt tccagcctcg tttggaaagt
     7381 ttcattgcca gacgggactc ctgcaatcgt caagggattg aaacctatag aagacattgc
     7441 tgatgaactg cgcggggccg actatctggt atggcgcaat gggaggggag cagtccggtt
     7501 gctcggtcgt gagaacaatc tgatgttgct cgaatatgcc ggggagcgaa tgctctctca
     7561 catcgttgcc gagcacggcg actaccaggc gaccgaaatt gcagcggaac taatggcgaa
     7621 gctgtatgcc gcatctgagg aacccctgcc ttctgccctt ctcccgatcc gggatcgctt
     7681 tgcagctttg tttcagcggg cgcgcgatga tcaaaacgca ggttgtcaaa ctgactacgt
     7741 ccacgcggcg attatagccg atcaaatgat gagcaatgcc tcggaactgc gtgggctaca
     7801 tggcgatctg catcatgaaa acatcatgtt ctccagtcgc ggctggctgg tgatagatcc
     7861 cgtcggtctg gtcggtgaag tgggctttgg cgccgccaat atgttctacg atccggctga
     7921 cagagacgac ctttgtctcg atcctagacg cattgcacag atggcggacg cattctctcg
     7981 tgcgctggac gtcgatccgc gtcgcctgct cgaccaggcg tacgcttatg ggtgcctttc
     8041 cgcagcttgg aacgcggatg gagaagagga gcaacgcgat ctagctatcg cggccgcgat
     8101 caagcaggtg cgacagacgt catactagat atcaagcgac ttctcctatc ccctgggaac
     8161 acatcaatct caccggagaa tatcgctggc caaagcctta gcgtaggatt ccgccccttc
     8221 ccgcaaacga cccctttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc
     8281 gagggcaacc agcccggtga gcgtcggaaa ggcgctggaa gccccgtagc gacgcggaga
     8341 ggggcgagac aagccaaggg cgcaggctcg atgcgcagca cgacatagcc ggttctcgca
     8401 aggacgagaa tttccctgcg gtgcccctca agtgtcaatg aaagtttcca acgcgagcca
     8461 ttcgcgagag ccttgagtcc acgctagatc tatctcatct gcgcaaggca gaacgtgaag
     8521 acggccgccc tggacctcgc ccgcgagcgc caggcgcacg aggccggcgc gcggacccgc
     8581 gccacggccc acgagcggac gccgcagcag gagcgccaga aggccgccag agaggccgag
     8641 cgcggccgtg aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac
     8701 gggcgtctga cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga
     8761 gtgggttgcg ctccggcagc ggtcctgagc ggtcctgatc aatcgtcacc ctttctcggt
     8821 ccttcaacgt tcctgacaac gagcctcctt ttcgccaatc catcgacaat caccgcgagt
     8881 ccctgctcga acgctgcgtc cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac
     8941 agcggcgaga gcggagcctg ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg
     9001 gcctgctcct caagcacggc cccaacagtg aagtagctga ttgtcatcag cgcattgacg
     9061 gcgtccccgg ccgaaaaacc cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc
     9121 atctgcggtg cgcccggtcg cgtgccggaa tggatgcgcg cgccatcgcg gtaggcgagc
     9181 agcgcctgcc tgaagctgcg ggcattcccg atcagaaatg agcgccagtc gtcgtcggct
     9241 ctcggcaccg aatgcgtatg attctccgcc agcatggctt cggccagtgc gtcgagcagc
     9301 gcccgcttgt tcctgaagtg ccagtaaagc gccggctgct gaacccccaa ccgttccgcc
     9361 agtttgcgtg tcgtcagacc gtctacgccg acctcgttca acaggtccag ggcggcacgg
     9421 atcactgtat tcggctgcaa ctttgtcatg cttgacactt tatcactgat aaatataata
     9481 tgtccaccaa cttatcagtg ataaagaatc cgcgcgttca atcggaccag cggaggctgg
     9541 tccggaggcc agacgtgaaa cccaacagac ccctgatcgt aattctgagc actgtcgcgc
     9601 tcgacgctgt cggcatcggc ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg
     9661 ttcactcgaa cgacgtcgcc gcccactatg gcattctgct ggcgctgtat gcgttgatgc
     9721 aatttgcctg cgcacctgtg ctgggcgcgc tgtcggatcg tttcgggcgg cggccggtct
     9781 tgctcgtctc gctggccggc gctgctgtcg actacgccat catggcgacg gcgcctttcc
     9841 tttgggttct ctatatcggg cggatcgtgg ccggcatcac cggggcgact ggggcggtag
     9901 ccggcgctta tattgccgat atcactgatg gcgatgagcg cgcgcggcac ttcggcttca
     9961 tgagcgcctg tttcgggttc gggatggtcg cgggacctgt gctcggtggg ctgatgggcg
    10021 gtttctcccc ccacgctccg ttcttcgccg cggcagcctt gaacggcctc aatttcctga
    10081 cgggctgttt ccttttgccg gagtcgcaca aaggcgaacg ccggccgtta cgccgggagg
    10141 ctctcaaccc gctcgcttcg ttccggtggg cccggggcat gaccgtcgtc gccgccctga
    10201 tggcggtctt cttcatcatg caacttgtcg gacaggtgcc ggccgcgctt tgggtcattt
    10261 tcggcgagga tcgctttcac tgggacgcga ccacgatcgg catttcgctt gccgcatttg
    10321 gcattctgca ttcactcgcc caggcaatga tcaccggccc tgtagccgcc cggctcggcg
    10381 aaaggcgggc actcatgctc ggaatgattg ccgacggcac aggctacatc ctgcttgcct
    10441 tcgcgacacg gggatggatg gcgttcccga tcatggtcct gcttgcttcg ggtggcatcg
    10501 gaatgccggc gctgcaagca atgttgtcca ggcaggtgga tgaggaacgt caggggcagc
    10561 tgcaaggctc actggcggcg ctcaccagcc tgacctcgat cgtcggaccc ctcctcttca
    10621 cggcgatcta tgcggcttct ataacaacgt ggaacgggtg ggcatggatt gcaggcgctg
    10681 ccctctactt gctctgcctg ccggcgctgc gtcgcgggct ttggagcggc gcagggcaac
    10741 gagccgatcg ctgatagagg tttatagcgg cttcgttccc ttccgcaacc gtcagcatga
    10801 tctgctccac atgctcgcgg ccatagtcta tcaccgcccg cagcagcata ccggcgaccc
    10861 ctcggcctcg ctgttcgggc tccacgaaaa cgccggacag atgcgccttg tgagcgtcct
    10921 tggggccgtc ctcctgtttg aagaccgaca gcccaatgat ctcgccgtcg atgtaggcgc
    10981 cgaatgccac ggcatctcgc aaccgttcag cgaacgcctc catgggcttt ttctcctcgt
    11041 gctcgtaaac ggacccgaac atctctggag ctttcttcag
//
