LOCUS       NZ_CP025235             6761 bp    DNA     circular CON 06-MAR-2024
DEFINITION  Salmonella enterica subsp. enterica serovar Newport str.
            USDA-ARS-USMARC-1926 plasmid pSNE1-1926, complete sequence.
ACCESSION   NZ_CP025235
VERSION     NZ_CP025235.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02716896
            Assembly: GCF_004136095.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Newport str.
            USDA-ARS-USMARC-1926
  ORGANISM  Salmonella enterica subsp. enterica serovar Newport str. USDA-AR...
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Nguyen,S.V., Harhay,D.M., Bono,J.L., Smith,T.P.L., Fields,P.I.,
            Dinsmore,B.A., Santovenia,M., Kelley,C.M., Wang,R., Bosilevac,J.M.
            and Harhay,G.P.
  TITLE     Comparative Genomics of Salmonella Newport
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6761)
  AUTHORS   Nguyen,S.V., Harhay,D.M., Bono,J.L., Smith,T.P.L., Fields,P.I.,
            Dinsmore,B.A., Santovenia,M., Kelley,C.M., Wang,R., Bosilevac,J.M.
            and Harhay,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2017) Meat Safety and Quality Research Unit,
            USDA-ARS-USMARC, State Spur 18D, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 145.91x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/06/2024 01:51:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,867
            CDSs (total)                      :: 4,746
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 44 of 119
            Pseudo Genes (incomplete)         :: 90 of 119
            Pseudo Genes (internal stop)      :: 19 of 119
            Pseudo Genes (multiple problems)  :: 31 of 119
            Pseudo Genes (short protein)      :: 1 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP025235.1.
            Bacteria and source DNA available from USMARC.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Salmonella enterica subsp. enterica serovar
                     Newport str. USDA-ARS-USMARC-1926"
                     /mol_type="genomic DNA"
                     /strain="USDA-ARS-USMARC-1926"
                     /serovar="Newport"
                     /isolation_source="bovine pre-evisceration carcass"
                     /host="Bovine"
                     /sub_species="enterica"
                     /db_xref="taxon:1454619"
                     /plasmid="pSNE1-1926"
                     /geo_loc_name="USA: BIFSCo Region 2"
                     /collection_date="2006-01"
                     /collected_by="USMARC"
     gene            1..195
                     /locus_tag="AW82_RS24280"
                     /old_locus_tag="AW82_24840"
     CDS             1..195
                     /locus_tag="AW82_RS24280"
                     /old_locus_tag="AW82_24840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041631.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQIRFEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            860..973
                     /locus_tag="AW82_RS25530"
     CDS             860..973
                     /locus_tag="AW82_RS25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071588254.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLSPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(1376..4012)
                     /locus_tag="AW82_RS24300"
                     /old_locus_tag="AW82_24860"
     CDS             complement(1376..4012)
                     /locus_tag="AW82_RS24300"
                     /old_locus_tag="AW82_24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443154.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:0016787 -
                     hydrolase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="WP_052512605.1"
                     /translation="MAKKPKSAGREKLLFNKLKQFDEPGLFHDNYQTPDYIQANLKDIL
                     RPYQHSALRYLHYTQRKRDDALLHYRHLLFHMATGAGKTMVMAGTILYLFKEFGYQNFI
                     FFVHTDAIIQKTRENLLNPQSPKYLFSHELEIDGEKITIAPVDIFPSVPERNTIYLKLS
                     TIHKMHDELNSYRENSITYEDLKEIPLVLLGDEAHHFNAGTKARGKSKTSPENEEQTWE
                     RTIENILDLRPDNRLFEFTATIDLANKDIGQKYRDKVVYQYDLKQFMSDGYSKKVMLLE
                     ANQNDSDKMLDAVLLSQYRKLTAADHGITGFKPVILFKSNKIAISKAKQEEFSQLIAAM
                     TPESIRRHLANKKLQLSSDTSIWHKVIQRYADSDLVTVTGQIQEDFNDFNLLNVNKSDL
                     LEENPVLLNTLEEVDNPVRAVFAVAKVNEGWDVLNLYDIVRISEQASSSKTSTDSEAQL
                     IGRGARYYPFIYDGQRSFTRRFDNSTKDLSVLEQLHYHTINEPAYIKTLHASLEQADID
                     VHQDGGGTVEHARLKEDFKKSAVYQAGKLYFNEVEEIERSSRNWETYSLETRFEIQYQI
                     ACEESLDNLTGTKGGIIKPEPLVLDERFYRKAMQRISFYALDNLKRFFPKLTGIREFIR
                     SDAYLGKLKITVSVPQSLDFTTVPAKDKLHLLENVLLRISENVRRNDQKVKGTYRFISQ
                     PVKEVIKDYSLHIDPSVAINQKITAAPTIGKKWYVYDNAILNQLEHRLIKMLEAFMPKL
                     KARYDDIYVLRNDEQSTRFKLTEFGGVRGFMPDFIMILTRHSDNTYWQVFLEPKGDDRL
                     LDDAWKERMLETLNDRERIVIDENEHVRLVGIKFFANSQMDVFVSDMQNKLNDGESLET
                     SSLSLPL"
                     /name="DEAD/DEAH box helicase family protein"
     gene            complement(4017..5882)
                     /locus_tag="AW82_RS24305"
                     /old_locus_tag="AW82_24865"
     CDS             complement(4017..5882)
                     /locus_tag="AW82_RS24305"
                     /old_locus_tag="AW82_24865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816428.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="WP_021558993.1"
                     /translation="MQTELIKELNKVLKAFPQFWNGEELQRSMVSDAISQKQPDLIKAL
                     VDNEKVRSVYGVDIDGIFIFDFDKLCGLLKYKEYWANSFTKYRNKVGLTSEGKYLDYSS
                     DVVLDFPFKDCILEGGMTKENQGKDEIYYNEIIAMDEVDSLLSPKAFTNAKRYTKEGVK
                     ENITKFNDDENLIIKGNNLIALHSIKKLYAGKVKLIYIDPPYNTGSDSFKYNDRFNRST
                     WLTFMKNRLEVAKEMLSDDGFIFIQCDDNESAYLKVLCDSIFGEENYRNSIYWHRTYAG
                     KTVKSKLPWNTDTILFYSKKESSLLEPYTAELSAADIKSYNKDDFDGRGKYNTVSLQKT
                     SSPGPQTTYDYIDNTGKKWSCPEKGWRMTKEKLKALENDNRLYITDKTIREKYYLKERK
                     EIGKQIDNFWHDIGNLNRVSDVAFIDGQKTEKLMQRIISLASKENDIVLDFHLGSGTSA
                     AAAHKMGRRYIGIEQMDYINEITVPRLQKVIEGEQGGISKDVNWQGSGSFVYVELVELN
                     AYFIHEIQKAQSTEELEKLFAVMKTEAYLNYQVALENVLSAEYEVDGILHKVAFSELEL
                     HEQKQLLIEILDKNQLYVNVSDMEDSDLNISESDKAFTRSFYGME"
                     /name="site-specific DNA-methyltransferase"
     Typing          complement(276..361)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.58e-38"
     Typing          complement(6248..6503)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.248"
                     /coverage="89.0"
                     /evalue="2.69e-98"
     Typing          complement(6628..6726)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          379..932
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.075"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtgaacaaac aacaacaaac tgcgctgaat atggcgcgat ttatcaaaag ccagagcctg
       61 acactgcttg aaaaactgga tgctctggat gccgacgaac aggcggccat gtgcgaacga
      121 ctgcacgaac tcgcagaaga actccagaac agcatccaga ttcgctttga ggccgaaagt
      181 gaaacaggaa cataacgaag ctgacggagg cggtcacggc tttcccgtgg gcgttttcgt
      241 cagcagacaa gcccgtcagg gcgcgtcagg gggttttagc gggtgtcggg gcgcagccat
      301 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagtgcag
      361 attgtatgaa aagtacacca taaccgatgt gaaatgccgc acagatgcgt aaggagaaaa
      421 tgcaacttcc gacgctcttc cgcttcctcg ctcactgact cgctacgctc ggtcgttcgg
      481 ctgcggcgag cggtatcaac tcacgcaaag acagtaatta gattatccac cgaatcaggg
      541 gataaggccg gaaagaacat gtgagcaaaa gagcaggaac cggaaaaagg ccgcgccgct
      601 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      661 gagtcggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      721 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      781 gggaagcgtg gcgctttctc atagctcacg ctgttggtat ctcggttcgg tgtaggtcgt
      841 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      901 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      961 cactggtaac tgatttagag gagtttgtct tgaagttatg cacctgttaa ggctaaactg
     1021 aaagaacaga ttttggtgac tgcgctcctc caagccagtt acccggttag ataatctgcc
     1081 aggattagct aaccttcgaa aaaccacctg ccagggtggt tttttcgttt acaggctgca
     1141 gattacgcgc agaaaaaaag gatcacaaga ggatccttga atcttttccc cctcatcccc
     1201 ccccccaggc ataaacatag cgaacggttt ttaaccacca ttcaaaacta atgattcgca
     1261 acagcaagaa caaacaatgc aaccattaca ggagcaatca tcacctccct gggaggtagt
     1321 tcgagataac aataaaataa cggagcgtaa tgctccgtta ttttattgtc gtgacttaca
     1381 gtggtaggga gagcgaggag gtttcgagcg attctccatc attcagcttg ttctgcatgt
     1441 cgctgacaaa aacgtccatc tggctattgg caaagaattt aatcccaacc agtctgacgt
     1501 gctcattttc atcaattaca atgcgctccc tgtcattcag tgtttcgagc atccgttctt
     1561 tccatgcgtc atccagcaga cggtcatccc ccttcggttc aaggaacacc tgccagtaag
     1621 tgttgtctga atggcgcgtc agtatcatga tgaaatctgg cataaaccca cgcacgccgc
     1681 cgaactccgt gagcttaaag cgcgttgact gctcgtcgtt acgcagtacg taaatatcgt
     1741 cataacgcgc ttttagcttc ggcatgaatg cttccagcat ttttatcaga cgatgctcaa
     1801 gctgatttag tatcgcatta tcgtacacgt accatttttt accaatggtg ggagcggcgg
     1861 tgattttctg gttaatggct accgacgggt caatatgcaa actgtaatct ttaatcacct
     1921 ctttcaccgg ctggctgata aatcggtacg tgccttttac cttctggtca ttgcgacgca
     1981 cattctctga gatacgcagc aaaacgtttt ccagtaggtg cagcttatct tttgccggta
     2041 cggtggtgaa atccagtgac tgcggtacgc tgacggtgat tttcagctta ccaagatagg
     2101 cgtcactgcg tatgaactcc cgaatacctg tcagtttcgg gaagaagcgt ttcaggttat
     2161 ctagcgcgta aaagctgatt ctttgcatcg ccttgcggta aaaacgctcg tccagtacca
     2221 gtggttccgg ttttatgatc cccccctttg tgcctgtcag gttgtccagc gactcctcgc
     2281 aagcgatctg atactgtatt tcaaagcgtg tttccagcga gtaggtttcc cagtttcggc
     2341 tgcttctctc aatctcttcc acttcgttaa agtacagctt acccgcctga tacacggcgg
     2401 actttttaaa gtcttctttc aatctggcgt gttcaaccgt acctccgcca tcctgatgca
     2461 cgtcaatgtc cgcctgctcc agagaggcgt gcagtgtttt gatgtacgcc ggttcattga
     2521 tggtgtgata gtgcagttgc tccagtacgc tcaaatcctt cgtactgtta tcaaaacggc
     2581 gggtgaagct acgttgaccg tcataaatga acgggtagta acgcgcacct cgcccaataa
     2641 gctgcgcttc gctgtccgtg ctggttttgc tgctgctcgc ctgctcgctt atgcgaacaa
     2701 tatcgtagag gttaagtacg tcccagcctt cattcacctt cgcgacagca aagacggctc
     2761 tcactggatt atcgacttct tccagtgtat tcagcaggac ggggttttcc tccagcaggt
     2821 ctgacttgtt gacgttcagc aggttaaagt cgttaaagtc ctcctgaatt tgtccagtca
     2881 ccgtcactaa atcactgtca gcgtaacgct gaatcacttt atgccagata ctggtgtcag
     2941 agctgagctg gagttttttg ttagccagat gacgtctgat gctctcaggt gtcatggcgg
     3001 caatcagttg cgaaaactcc tcctgcttcg cctttgatat cgcaatctta ttcgacttga
     3061 acagaatgac cggtttaaag ccggtgatgc cgtgatctgc tgccgtcagt ttacggtact
     3121 ggcttaacag taccgcatcg agcatcttgt cgctgtcgtt ctggtttgct tccaacagca
     3181 tgaccttttt cgagtaaccg tctgacatga actgcttcag atcatactga tataccacct
     3241 tgtcacggta tttctgtccg atatctttgt ttgcaaggtc aatggtagcg gtgaactcga
     3301 acaggcggtt atccggtcgc aggtcgagga tgttctcaat cgtacgctcc caagtctgct
     3361 cctcgttctc cggtgaggtt ttcgacttgc ctctagcttt ggtgccggcg ttgaagtgat
     3421 gcgcctcgtc accgagtagc acaagcggga tttcttttaa atcctcgtag gtaatgctgt
     3481 tctctcgata gctgttcagc tcatcgtgca tcttatggat agtcgagagc ttgaggtaaa
     3541 tcgtgttgcg ttccggtacg gacgggaata tatctaccgg tgcgatggtg attttttccc
     3601 cgtcgatttc cagctcatga ctgaacaggt acttcggcga ttgcgggtta agtaggttct
     3661 ctcgcgtttt ctggataatc gcgtcggtat gtacgaaaaa gatgaagttc tgatacccga
     3721 actctttaaa tagatagagt atcgttcctg ccataaccat cgttttccct gcaccggttg
     3781 ccatgtgaaa cagcaggtga cggtaatgca gaagcgcgtc atcacgttta cgttgcgtgt
     3841 aatgcaggta acgcagtgcg ctgtgctgat agggacgcag gatatctttc aggtttgcct
     3901 gaatatagtc cggcgtctga tagttatcgt ggaacagtcc tggttcatcg aactgcttca
     3961 gcttgttaaa cagcagcttc tctctacctg ctgacttcgg tttcttcgcc atgccgtcac
     4021 tccattccgt agaagctgcg ggtaaaggct ttatcactct ctgaaatgtt caggtcgctg
     4081 tcctccatat cagagacatt cacgtagagc tggttcttat ctaggatttc aatcaaaagc
     4141 tgcttctgct catgcagttc cagttcactg aatgctacct tatggaggat cccatccact
     4201 tcgtactcag cggacagcac attttccaga gccacctgat agttgagata tgcctctgtc
     4261 ttcatcacgg caaagagttt ttcgagttct tccgtggact gcgctttctg gatttcatgt
     4321 atgaagtaag cgttcagttc cactagttca acgtagacaa agctgccgct cccctgccag
     4381 ttgacgtctt ttgagatacc tccctgctca ccctcaatga ctttttgcaa acgtggcacg
     4441 gtgatctcat tgatgtaatc catctgctca ataccgatat agcgacgtcc cattttatgg
     4501 gctgctgctg cgctagttcc gctgccgaga tgaaagtcca agacgatatc attttctttg
     4561 gaagcaaggc taattatccg ttgcattaat ttttcagttt tttgtccatc aataaaagca
     4621 acatcggaaa ccctatttaa attcccaatg tcatgccaaa aattatctat ttgtttcccg
     4681 atctcttttc tttctttaag ataatatttt tctcgtattg ttttgtcagt gatatatagc
     4741 ctattgtcgt tttccaaagc ctttaacttt tcctttgtca ttctccaccc tttttcaggg
     4801 caactccatt tcttacctgt attatctata taatcataag ttgtttgcgg ccctggagag
     4861 cttgtttttt gcaagctaac agtgttgtat ttaccccttc catcaaaatc atccttgtta
     4921 taggatttaa tgtcagcagc actaagttct gctgtatatg gctctaataa tgaactttct
     4981 tttttagaat aaaacaatat tgtatctgta ttccatggca acttactttt tacagttttc
     5041 cctgcgtagg ttctatgcca atagattgag tttctataat tctcttctcc aaaaattgaa
     5101 tcacataaaa ctttaagata tgctgattca ttatcatcgc attgaataaa aataaagcca
     5161 tcatcggaaa gcatttcttt agccacctca agcctatttt tcatgaatgt caaccatgtt
     5221 gagcgattga atcgatcatt atacttaaag ctatcactcc ctgtattgta aggcgggtct
     5281 atataaatta actttacttt tcctgcgtaa agttttttta ttgaatgtag tgcaatgaga
     5341 ttgtttccct taattattaa attttcatca tcgttaaatt tagtgatatt ttcttttacc
     5401 ccctctttgg tatatcgctt agcattagtg aacgccttgg gagaaagcaa agagtcaact
     5461 tcatccattg ctatgatttc attataataa atctcatctt ttccctgatt ttctttggtc
     5521 attccacctt caagaataca atccttgaaa ggaaaatcca gcaccacatc agaactgtaa
     5581 tcaaggtatt taccttcgct agttaatccc actttgttcc ggtatttagt aaagctatta
     5641 gcccaatatt ctttgtattt tagaagacca cataatttgt caaaatcaaa gataaaaatg
     5701 ccatcaatat ccacaccata aacagagcgg actttttcat tatcgactaa tgccttgata
     5761 agatctggct gcttttgact gatagcgtca ctcaccattg aacgttgtaa ttcctctcca
     5821 ttccagaatt gcgggaacgc ttttaaaacc ttgttaagtt cttttattaa ttcggtctgc
     5881 atgtcatggc cttatgactg ttaaatattg gaatgattgt aaggtatagc gttcggcgct
     5941 ggaacccaac agcgatgagt tggattcagt tgcgggaaaa tgcaccagaa cgagtagtat
     6001 ttaacataaa cgctcttgcg cgcaccgcta aaacgcgctg aacgcgattc tgccgcaaac
     6061 accgcaaaaa gagatgtttt ccccggggta aaccggaaaa atgcgtcagg atcactttca
     6121 gctcactgca tagctatgca tgaaagtgaa tgacgatcgg tttgggcgca tacagtaccc
     6181 atgagacaca ttttcggaca ttttcagttc gtgaatccct gactcattgc tacatatctg
     6241 aacagacacc cctagcagcg cccccagggg cgtcctgtaa aaaaacacac cgcgccgcta
     6301 gcagcacccc taatataaga tgatgttttt tatgaaaata gtcagtacca cccctaaaaa
     6361 gcggtgtcgg cgcggtgttg tagctgcgcc aacaccgctt ttttaaatat cacaaagaga
     6421 gcaagaagaa ctaatttgtc ataactctct atttataaag aaaaatcagc aaaaacttgt
     6481 ttttgcgtgg ggtgtgggca tttcaggcat gagaacagac tcgcgttttt ccggagtatc
     6541 agcggaatta ttgattaaga aacactcgac gcagagcgaa attttatgca acagcggctg
     6601 cactgcacag ggattattta tcagttgata tgctgtgcca tgatacgtcc taaagaaaca
     6661 ggacaggatg tgcaattgta ataccgtcgc ccgcaacgtc ttacaattac cacctgctca
     6721 gggtcccccc gacacccgga actgacggag aacaacggaa g
//
