LOCUS       NZ_CP024803             2088 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain AMA1167 plasmid pAMA1167-4, complete
            sequence.
ACCESSION   NZ_CP024803
VERSION     NZ_CP024803.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07983336
            Assembly: GCF_002803905.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Overballe-Petersen,S., Roer,L., Ng,K., Hansen,F., Justesen,U.S.,
            Andersen,L.P., Stegger,M., Hammerum,A.M. and Hasman,H.
  TITLE     Complete Nucleotide Sequence of a ST410 Escherichia coli strain
            carrying blaNDM-5 on an IncF multidrug resistance plasmid and
            blaOXA-181 on an IncX3 plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Overballe-Petersen,S., Hasman,H. and Hammerum,A.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2017) Microbiology and Infection Control, Statens
            Serum Institut, Artillerivej 5, Copenhagen S 2300, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2017
            Assembly Method                   :: other v. October-2017
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: other; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002803905.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 01:59:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,826
            CDSs (total)                      :: 4,709
            Genes (coding)                    :: 4,460
            CDSs (with protein)               :: 4,460
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 249
            CDSs (without protein)            :: 249
            Pseudo Genes (ambiguous residues) :: 0 of 249
            Pseudo Genes (frameshifted)       :: 79 of 249
            Pseudo Genes (incomplete)         :: 163 of 249
            Pseudo Genes (internal stop)      :: 51 of 249
            Pseudo Genes (multiple problems)  :: 36 of 249
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024803.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AMA1167"
                     /isolation_source="liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pAMA1167-4"
                     /geo_loc_name="Denmark"
                     /collection_date="2015"
                     /collected_by="Statens Serum Institut"
     gene            634..1599
                     /locus_tag="CUC42_RS24510"
                     /old_locus_tag="CUC42_24500"
     CDS             634..1599
                     /locus_tag="CUC42_RS24510"
                     /old_locus_tag="CUC42_24500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_039064478.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLAIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            1603..1869
                     /locus_tag="CUC42_RS24515"
                     /old_locus_tag="CUC42_24505"
     CDS             1603..1869
                     /locus_tag="CUC42_RS24515"
                     /old_locus_tag="CUC42_24505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          1028..1259
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 gcctcgcttt gctcgttgct caacgcctca cagacacgga ttaaaatccg catccgttca
       61 ccgtttttta aagtccgtta aaagcatgat gccatctccg agagttaatc tcgtcaaatg
      121 ctaaatcgtg ggggtcccct ttggggttcc gatttagtga ttgacgacac caccgattaa
      181 aaaacttatg cggggtggat ggtttcacga agtgaggcca tccacctgta agacagggtt
      241 ttgtttttat cccctgtttt ggtgatcggg tgtgtggaaa aggttggggt aagccgttcg
      301 gggtgcttgt tttggggggt taaaattgtg gttatttttt gcgcaattct cgcgcgtgat
      361 ccttgtattt atacttaagg gataaatggc gggtatgaaa tagtggttta gcccagtaat
      421 gacgaggctt tgagtgggtt ttgacaggtc aaagaaaatg gagcagaatt gaggcgtttt
      481 taatcggcgt tggggagtgc gtcaacactc cccaacattt cgaatgtgtc acctcagcgg
      541 caaactctgg tgacatgtac tggctcgcaa tgcacaggta cgtgatgaat ataccacatc
      601 aaatcacagc ctgcccagat cggagcaggc ttaatgtcag aagataaatt cctttcggac
      661 tacagccccc gtgatgcagt ttgggatacc cagcgcacgc ttaccgattc tgtcgggggt
      721 atctaccaga ctgctgctga attcgagcgc tatgcactcc gtatggcctc ctgtagcggt
      781 ttgttacgtt ttggttggtc taccatcatg gaaaccggag aaacgcgcct acggcttcgt
      841 agtgcgcaat tttgccgtgt ccgtcattgc cctgtctgcc agtggagaag aaccctcatg
      901 tggcaagccc gtttttatca ggctctaccg aaaatcgttg tggattaccc gtcttcccga
      961 tggttgtttc tgacgttaac tgtcaggaac tgcgagatag gtgaacttgg aacagtcctt
     1021 acagcaatga atgcggcgtt taagcgaatg gaaaagcgaa aggagctatc acctgttcag
     1081 gggtggatca gggctacgga ggtgacgcga ggtaaggatg gcagcgcaca tccgcatttt
     1141 cactgtctgc tgatggtgca accttcttgg tttaaaggga agaactacgt taagcacgaa
     1201 cgttgggtag aactctggcg cgattgcttg cgggtgaact atgagccgaa tatcgatatt
     1261 cgggcagtaa aaactaagac aggtgaggtt gtggccaacg ttgccgagca actgcaaagc
     1321 gcggttgctg aaacgctgaa atactccgtt aaaccggaag atatggcaaa cgatcctgag
     1381 tggtttcttg agctgacgcg gcagcttcac aagcgccgtt ttatctcgac cggtggagcg
     1441 ctaaaaaacg tcctccagtt ggatcgagaa accaatgagg atcttgccat tgccgacgat
     1501 gtaggggatg gcactgatga cgggaagcgg acggcgtttg tctgggattc aggtaaacgg
     1561 cgttacaaac gcgcccctga gaaggataaa tcggattaac gtatgaatat taatattgaa
     1621 tacctgaatg gaaataagac tattggttta ttttttttaa gaagtgaagc ggtgattcct
     1681 gacaggttta aaaaccttat tttgcttatt gatggattaa gttttggcac atttggtttt
     1741 catccgcacg aaggttttga ggatgaatta attttatata ttcagaaaac aaacgagagg
     1801 gtaaaaactc tttttgtgaa aatagattta aacggtatca agtttggatt tttaagaacg
     1861 cattcttagt tctggaaaag agccagcggc aggctgaggt gataggtacg agattgcatg
     1921 caatctctag tgctctgtct atcctgcatt atcctcagca ttatcctcag ccttgccaac
     1981 tcgacaccaa tgcaggatag acaatccgat gtcaaatgtt aacactctgc gagtggtaca
     2041 ttttccccgg attatcgtcc tgagcctgcc gctggctctc tttctacc
//
