LOCUS       NZ_CP024722             5167 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli isolate NQ3 plasmid p2NQ3, complete sequence.
ACCESSION   NZ_CP024722
VERSION     NZ_CP024722.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07980821
            Assembly: GCF_002763995.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Tan,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2017) College of Veterinary Medicine, Huazhong
            Agricultural University, Huazhong Agricultural University, Wuhan,
            Huibei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 06-NOV-2015
            Assembly Method                   :: SMRT v. RS HGAP V2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 305.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002763995.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 02:22:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,245
            CDSs (total)                      :: 5,131
            Genes (coding)                    :: 4,921
            CDSs (with protein)               :: 4,921
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 0 of 210
            Pseudo Genes (frameshifted)       :: 76 of 210
            Pseudo Genes (incomplete)         :: 155 of 210
            Pseudo Genes (internal stop)      :: 45 of 210
            Pseudo Genes (multiple problems)  :: 54 of 210
            Pseudo Genes (short protein)      :: 1 of 210
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024722.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="NQ3"
                     /host="Bos mutus"
                     /db_xref="taxon:562"
                     /plasmid="p2NQ3"
                     /geo_loc_name="China:NaQu, Xizang"
                     /collection_date="2015-03-01"
     gene            complement(join(4856..5167,1..87))
                     /locus_tag="CUB98_RS27790"
                     /old_locus_tag="CUB98_27790"
     CDS             complement(join(4856..5167,1..87))
                     /locus_tag="CUB98_RS27790"
                     /old_locus_tag="CUB98_27790"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            264..1190
                     /locus_tag="CUB98_RS27795"
                     /old_locus_tag="CUB98_27795"
     CDS             264..1190
                     /locus_tag="CUB98_RS27795"
                     /old_locus_tag="CUB98_27795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1193..1726
                     /locus_tag="CUB98_RS27800"
                     /old_locus_tag="CUB98_27800"
     CDS             1193..1726
                     /locus_tag="CUB98_RS27800"
                     /old_locus_tag="CUB98_27800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(1792..2001)
                     /locus_tag="CUB98_RS27805"
                     /old_locus_tag="CUB98_27805"
     CDS             complement(1792..2001)
                     /locus_tag="CUB98_RS27805"
                     /old_locus_tag="CUB98_27805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            3738..4652
                     /locus_tag="CUB98_RS27810"
                     /old_locus_tag="CUB98_27810"
     CDS             3738..4652
                     /locus_tag="CUB98_RS27810"
                     /old_locus_tag="CUB98_27810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271348.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     Typing          3739..4513
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          265..1187
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatctgtctc tgtcgtgcgg tctgttctcc gagcttcctg atgaatccga tccgtttctt
       61 cagccattcg atgttgctga tgcgcatgtt cgtgatgctc cctggtgttt ttttgcggtg
      121 tgttgtggtc gtgcagtata tcgcttgcgg tcggcgggtg cagggggtat cttccgtgct
      181 aattcgtaaa attagaagga agcgcactta tatgtttctc tccttcgtcg agaaacagtg
      241 cgctcctgca gagggctgaa accgtggcaa cctatcacct cagcgttaaa tttggtggca
      301 aggggcaggc tgcaaaccac gccgattaca tcgagcgcaa agagaaatac cgcgaccgtc
      361 aggatttgga atattccgca cacggaaaca tgccggaatg ggccagggat aatccttccc
      421 atttctggca ggcggctgac cagtttgaac gtgcgaacgg ttcgacctat agggaacttg
      481 aaattgcatt accccgtgaa ctgacaccgg aacagcgcct tgaactggtg caggattttg
      541 tacgtcagga agctggcgaa cggcacgcct ggagttttgc cattcacaat ccgaaagcga
      601 gcattgacgg cggcgaacag cctcatgcgc atatcatgat gagtcagcgt gtgaatgatg
      661 gcattgaacg tacaccggaa cagtatttcc ggcgctataa cgccaggtat cccgagcggg
      721 gaggtgcgaa aaaggattcc ggaagcctga ctctcactca gcaaaaagag cagttgcggg
      781 aattgcgaaa acgctgggag gtgaagcaca acgaacatat gagaaagcat ggctttgaaa
      841 ggggcttcat tgactgccgg acgctgaaag aacagggcat tgaacgacgt cccgaagtgc
      901 atctgggttt tgaagctgcc ggacggcttg atgatgccca gcgtcatgac atcatgcaaa
      961 aacggtctga accggactat tcccgccatc ttgaacaggc gcgaacggtc gggcatgaca
     1021 tcagggaatg gctggacact ccggttaagc aacatgaact ggtcagaagg ctgcaacatg
     1081 atgttgcgcg ggagcatggc tttgacatga gagacagagt gagcagacag aaccgaactg
     1141 cacctgaacg ggttaaatca ccgtcacgtg gattttctat gggattctga acatggccag
     1201 cacagaagaa aaattactca cgctcctgtt atcggtggag catctgcaac ataccgcaat
     1261 ggagcaacag aaagctgttg ctgattctct ggctgtggca agggaaactc ttcgcggata
     1321 tcgggatatt tctgacggtg tggcaaaaca ggtcagggat gaagtcagaa atgaggtgca
     1381 gaagctggac gtggcaggac tgataggtga gcgcatcagc caggattttc agcggctgga
     1441 agagtcagtc gcaaacatga aggcaaacgc ggatgccctg aacagggata ttgcgacaat
     1501 tcacagcaat atcgtgcagg aatacaacac ccttaaaggg tatagctgga aatggctggg
     1561 tggggtgtca gtggcttttc tggttatgtt ctgtgtgctc ggatggggaa tgaaaacaat
     1621 actcgacagg aactacaacg ccacagcggc ggtgtatcag aaagttgtga cactggaaga
     1681 taaactggtg gagaaacctg tcacggggaa gagccggaaa aactgacgcg cttcgcttgt
     1741 ccaacgcctc acgacccgcg aaaggatttc gcgaaccgtt caccgttttt ttcaatatga
     1801 ttctgtctgc tttggtcgat aaagcagacg atagtattcc agccttcgta tatataacgg
     1861 tacgtgctca tcagtaagag ctggcggtat tgtgttatca gggggaagtt ttttcagata
     1921 ctcacgaaat gcctggcttg atgccataaa atctacagta gcatcgataa tatgtggggt
     1981 gttttcacct aattttgcca tgatgataat ccttatatta tcacggtttg tgaatgatag
     2041 ggtgtcacta ttatttatgc gttagtgaac cagaaaaaat cccccgttgg cataggggga
     2101 tatgtaagga gaaataactt cgcaggtgtt cttgtttgta taacgtgaaa agactgcacg
     2161 caacttaata agtctgccgt acttactttc agttttcaac ataaggttta taacaatgta
     2221 tggaatggaa gtgagcttta aggtttcagc tctaaaaaat aaaatattta ttagttggct
     2281 ggttgttgtt cttgttctgt atgtatataa acactatcga catattatga ccgggaacat
     2341 tccttacccc ctctgcgctc gcagaggttt tttttgtcat taattaataa tgaactgaaa
     2401 tgcagttccg atacctccat ttgcacatta tccctgacat atggtaccgg aaccgcatta
     2461 attttatgtt ataagagttt atttttaatg tccagatggt ttaatagtat ttatatgtaa
     2521 ttagctgtat ggatagtact ggttttattg tataacttgt cgtttttttt tattgttttt
     2581 gtgtggtgtg ctaatcttta agttataaaa aaaccccttg gaatacaggg gcaaaagtaa
     2641 ggaaaacaaa tggatgctca gtttgtgtga cactttaaac taatggaact atagagtgga
     2701 ggttgttaca gtcgctttta taaaacagca accagttttt ttcaatagtc tgatgatggt
     2761 tgtgacagtt ttgtgatatt gttcaaaatg tttcttgttg aatgtttgtt ttttggtcta
     2821 ttggtgtctg gttttatttt ttctgctatt acatatcaag aaatgtgatt ggtgtagcaa
     2881 gagtggaatg cgatgtggat aaactaattg gttgtcgaat ctaatttact tagaaaacta
     2941 cacttgtttt tgtgtatgaa tttagtgaat ttgtctgtct atgtgttttc gtgcatttat
     3001 aaatccttcg tcaggatctt aaatttagtg ttgaatatgt agggcctgta cggtaattat
     3061 tatttagcgt agctacttca tcgttgttcg aacacaacca acaacctgta attgttgttt
     3121 ctcttggttc cccacttttg tggggttttt tatctcttcc gtgcatcgta aagcgcggac
     3181 tccttctgga aaggttgttt tagggcatca ctgaggcatt ttttgcgttt gtgaatattg
     3241 agcatccgat aactaaaata tttcccatag actctgattc ttgccaccga ccccaggtcg
     3301 gttttttttt gtcctggcga gatgtgaagt ttggaaacag gaaaccccgt cagagcgcca
     3361 cgcaccaggg gagaaaactt gtcgttttga gccgttcggt gttcagaacg cacgaaaccg
     3421 atcgcacatc gctttcgtgc atagttatgc aacgccatga aaacgattct gacgcgtttt
     3481 tctggtttgg cctggtgttt tcttgtcttt ttgcgttttt tgcgccagaa agcgactgag
     3541 ggcgttttaa ggggggcgta caacgggagt tatggtaaat ggatcgggtt ttcgggaagg
     3601 ttcgacagga tttgccgttg ggtgtagtgt aagcgactga aaaacaaacg cccctgaaat
     3661 catgtccaga tccggcaaga ttcaacatga aatacagagg gcgtgtaggt cgcataaccc
     3721 ggagagaatc gtaacacatg agcgccgcgc ttcaatactt cgacgaaaat ttaccccatc
     3781 gtccctatca cacggatgat ctcgcttttg gtcttcgcat ctccggcaaa gggcgtgcgc
     3841 ttcttgcgcg gtacatacag cagaaccagc ctcatgcgca gttctggctg gtttttgacg
     3901 ttgaccgcga gggggctgcg attgactgga gcgaccggaa cgctcccgcg ccgaacatca
     3961 ccgttaaaaa tcctgtgaac ggacacgctc acctgctcta cgcactcaac atcgccgtga
     4021 gaaccgcgcc tgatgcctcg gttaaggcgc tgaaatatgc cgcagcggtt gaacgttcgc
     4081 tgtgtgagaa actttgtgcg gatgtgaact acagcggcct gatttgcaaa aatccgttcc
     4141 acctggaatg gctggtgatg gagtggcgcg aggaagccta taccctcgat gaactggctg
     4201 attatctcga tttgagcgcc tcagagcgtc gtagcatcga taaacattac gggatggggc
     4261 gaaactgcca cctgttcgaa atgacgcgca aatgggctta cagggcgatt cgtcagggct
     4321 ggccagcgtt ctcacagtgg cttgaagcgg tgatccagcg tgtcgaaatg tacaatgcat
     4381 cgcttcccgt tccgctttca ctggctgaat gtcgggctat tggcaagagc attgcgaaat
     4441 acacgcacag gaacttcacg ccggaaactt tcgcacagta tgtggctgat acgcacacgc
     4501 cagaaattca ggctgcacgc ggtcgcaagg gcggttctaa gtctaagcgc agcacagtag
     4561 ctacatctgc acgcacgctg aaaccgtggg aggcattagg gatcagtcgc gcctggtatt
     4621 accaactgaa aaaacgaggt ctcgtagagt agaccaaata agcctatatc agataacagc
     4681 gcctttctgg cgtctttttg agcagcaggt tttttgccgt ttttccccgt tcagccctgc
     4741 aatggcgcgg ctttccgcgt gattgtggtg gtagtgctcg ccgcagtctc atgaccgagc
     4801 gtagcgagcg aatgagcgag gaagcaccag ggaatcggaa cacggatgga ggcacttacg
     4861 cgtctgggtt tggggatgtc tccagatcgt acttaagttc cttaagtgct tgtaatatcc
     4921 tgtctttttt atagcatagc tcgcctgttt ttgtgtctac cagtcctgct tcgatcagta
     4981 gcccagccgc gagaaagagc cggtgattgc gttcccgtcg ttgttttttt ccctcgattc
     5041 gtttttggac cgcctgtttt cgttcgctct cctgtgcctg taagtcgttt ttttccgctg
     5101 ttgccagtac gtggagaagt ttgcgttcct gttctgtgag tccggcttcg ttgtcgatca
     5161 ggtcaat
//
