LOCUS       NZ_CP024719             5162 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli strain LS4 plasmid p2LS4, complete sequence.
ACCESSION   NZ_CP024719
VERSION     NZ_CP024719.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07980822
            Assembly: GCF_002764015.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Tan,c.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2017) College of Veterinary Medicine, Huazhong
            Agricultural University, Huazhong Agricultural University, Wuhan,
            Huibei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 06-NOV-2015
            Assembly Method                   :: SMRT v. RS HGAP V2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 342.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002764015.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 01:55:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,245
            CDSs (total)                      :: 5,131
            Genes (coding)                    :: 4,917
            CDSs (with protein)               :: 4,917
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 214
            CDSs (without protein)            :: 214
            Pseudo Genes (ambiguous residues) :: 0 of 214
            Pseudo Genes (frameshifted)       :: 78 of 214
            Pseudo Genes (incomplete)         :: 157 of 214
            Pseudo Genes (internal stop)      :: 46 of 214
            Pseudo Genes (multiple problems)  :: 55 of 214
            Pseudo Genes (short protein)      :: 1 of 214
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024719.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LS4"
                     /host="Bos mutus"
                     /db_xref="taxon:562"
                     /plasmid="p2LS4"
                     /geo_loc_name="China:LaSa, XiZang"
                     /collection_date="2015-03-01"
     gene            1262..2176
                     /locus_tag="CUB99_RS27790"
                     /old_locus_tag="CUB99_27790"
     CDS             1262..2176
                     /locus_tag="CUB99_RS27790"
                     /old_locus_tag="CUB99_27790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271348.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(2380..2778)
                     /locus_tag="CUB99_RS27795"
                     /old_locus_tag="CUB99_27795"
     CDS             complement(2380..2778)
                     /locus_tag="CUB99_RS27795"
                     /old_locus_tag="CUB99_27795"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            2955..3881
                     /locus_tag="CUB99_RS27800"
                     /old_locus_tag="CUB99_27800"
     CDS             2955..3881
                     /locus_tag="CUB99_RS27800"
                     /old_locus_tag="CUB99_27800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            3884..4417
                     /locus_tag="CUB99_RS27805"
                     /old_locus_tag="CUB99_27805"
     CDS             3884..4417
                     /locus_tag="CUB99_RS27805"
                     /old_locus_tag="CUB99_27805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(4483..4692)
                     /locus_tag="CUB99_RS27810"
                     /old_locus_tag="CUB99_27810"
     CDS             complement(4483..4692)
                     /locus_tag="CUB99_RS27810"
                     /old_locus_tag="CUB99_27810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     Typing          1263..2037
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          2956..3878
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taagagttta tttttaatgt ccagatggtt taatagtatt tatatgtaat tagctgtatg
       61 gatagtactg gttttattgt ataacttgtc gttttttttt attgtttttg tgtggtgtgc
      121 taatctttaa gttataaaaa aaccccttgg aatacagggg caaaagtaag gaaaacaaat
      181 ggatgctcag tttgtgtgac actttaaact aatggaacta tagagtggag gttgttacag
      241 tcgcttttat aaaacagcaa ccagtttttt tcaatagtct gatgatggtt gtgacagttt
      301 tgtgatattg ttcaaaatgt ttcttgttga atgtttgttt tttggtctat tggtgtctgg
      361 ttttattttt tctgctatta catatcaaga aatgtgattg gtgtagcaag agtggaatgc
      421 gatgtggata aactaattgg ttgtcgaatc taatttactt agaaaactac acttgttttt
      481 gtgtatgaat ttagtgaatt tgtctgtcta tgtgttttcg tgcatttata aatccttcgt
      541 caggatctta aatttagtgt tgaatatgta gggcctgtac ggtaattatt atttagcgta
      601 gctacttcat cgttgttcga acacaaccaa caacctgtaa ttgttgtttc tcttggttcc
      661 ccacttttgt ggggtttttt atctcttccg tgcatcgtaa agcgcggact ccttctggaa
      721 aggttgtttt agggcatcac tgaggcattt tttgcgtttg tgaatattga gcatccgata
      781 actaaaatat ttcccataga ctctgattct tgccaccgac cccaggtcgg ttttttttgt
      841 cctggcgaga tgtgaagttt ggaacaggaa accccgtcag agcgccacgc accagggaga
      901 aacttgtcgt tttgagccgt cggtgttcag aacgcacgaa accgatcgca catcgctttc
      961 gtgcatagtt atgcaacgcc atgaaaacga ttctgacgcg tttttctggt ttggcctggt
     1021 gttttcttgt ctttttgcgt tttttgcgcc agaaagcgac tgagggcgtt ttaagggggg
     1081 cgtacaacgg gagttatggt aaatggatcg ggttttcggg aaggttcgac aggatttgcc
     1141 gttgggtgta gtgtaagcga ctgaaaaaca aacgcccctg aaatcatgtc cagatccggc
     1201 aagattcaac atgaaataca gagggcgtgt aggtcgcata acccggagag aatcgtaaca
     1261 catgagcgcc gcgcttcaat acttcgacga aaatttaccc catcgtccct atcacacgga
     1321 tgatctcgct tttggtcttc gcatctccgg caaagggcgt gcgcttcttg cgcggtacat
     1381 acagcagaac cagcctcatg cgcagttctg gctggttttt gacgttgacc gcgagggggc
     1441 tgcgattgac tggagcgacc ggaacgctcc cgcgccgaac atcaccgtta aaaatcctgt
     1501 gaacggacac gctcacctgc tctacgcact caacatcgcc gtgagaaccg cgcctgatgc
     1561 ctcggttaag gcgctgaaat atgccgcagc ggttgaacgt tcgctgtgtg agaaactttg
     1621 tgcggatgtg aactacagcg gcctgatttg caaaaatccg ttccacctgg aatggctggt
     1681 gatggagtgg cgcgaggaag cctataccct cgatgaactg gctgattatc tcgatttgag
     1741 cgcctcagag cgtcgtagca tcgataaaca ttacgggatg gggcgaaact gccacctgtt
     1801 cgaaatgacg cgcaaatggg cttacagggc gattcgtcag ggctggccag cgttctcaca
     1861 gtggcttgaa gcggtgatcc agcgtgtcga aatgtacaat gcatcgcttc ccgttccgct
     1921 ttcactggct gaatgtcggg ctattggcaa gagcattgcg aaatacacgc acaggaactt
     1981 cacgccggaa actttcgcac agtatgtggc tgatacgcac acgccagaaa ttcaggctgc
     2041 acgcggtcgc aagggcggtt ctaagtctaa gcgcagcaca gtagctacat ctgcacgcac
     2101 gctgaaaccg tgggaggcat tagggatcag tcgcgcctgg tattaccaac tgaaaaaacg
     2161 aggtctcgta gagtagacca aataagccta tatcagataa cagcgccttt ctggcgtctt
     2221 tttgagcagc aggttttttg ccgtttttcc ccgttcagcc ctgcaatggc gcggctttcc
     2281 gcgtgattgt ggtggtagtg ctcgccgcag tctcatgacc gagcgtagcg agcgaatgag
     2341 cgaggaagca ccagggaatc ggaacacgga tggaggcact tacgcgtctg ggtttgggga
     2401 tgtctccaga tcgtacttaa gttccttaag tgcttgtaat atcctgtctt ttttatagca
     2461 tagctcgcct gtttttgtgt ctaccagtcc tgcttcgatc agtagcccag ccgcgagaaa
     2521 gagccggtga ttgcgttccc gtcgttgttt ttttccctcg attcgttttt ggaccgcctg
     2581 ttttcgttcg ctctcctgtg cctgtaagtc gtttttttcc gctgttgcca gtacgtggag
     2641 aagtttgcgt tcctgttctg tgagtccggc ttcgttgtcg atcaggtcaa taatctgtct
     2701 ctgtcgtgcg gtctgttctc cgagcttcct gatgaatccg atccgtttct tcagccattc
     2761 gatgttgctg atgcgcatgt tcgtgatgct ccctggtgtt tttttgcggt gtgttgtggt
     2821 cgtgcagtat atcgcttgcg gtcggcgggt gcagggggta tcttccgtgc taattcgtaa
     2881 aattagaagg aagcgcactt atatgtttct ctccttcgtc gagaaacagt gcgctcctgc
     2941 agagggctga aaccgtggca acctatcacc tcagcgttaa atttggtggc aaggggcagg
     3001 ctgcaaacca cgccgattac atcgagcgca aagagaaata ccgcgaccgt caggatttgg
     3061 aatattccgc acacggaaac atgccggaat gggccaggga taatccttcc catttctggc
     3121 aggcggctga ccagtttgaa cgtgcgaacg gttcgaccta tagggaactt gaaattgcat
     3181 taccccgtga actgacaccg gaacagcgcc ttgaactggt gcaggatttt gtacgtcagg
     3241 aagctggcga acggcacgcc tggagttttg ccattcacaa tccgaaagcg agcattgacg
     3301 gcggcgaaca gcctcatgcg catatcatga tgagtcagcg tgtgaatgat ggcattgaac
     3361 gtacaccgga acagtatttc cggcgctata acgccaggta tcccgagcgg ggaggtgcga
     3421 aaaaggattc cggaagcctg actctcactc agcaaaaaga gcagttgcgg gaattgcgaa
     3481 aacgctggga ggtgaagcac aacgaacata tgagaaagca tggctttgaa aggggcttca
     3541 ttgactgccg gacgctgaaa gaacagggca ttgaacgacg tcccgaagtg catctgggtt
     3601 ttgaagctgc cggacggctt gatgatgccc agcgtcatga catcatgcaa aaacggtctg
     3661 aaccggacta ttcccgccat cttgaacagg cgcgaacggt cgggcatgac atcagggaat
     3721 ggctggacac tccggttaag caacatgaac tggtcagaag gctgcaacat gatgttgcgc
     3781 gggagcatgg ctttgacatg agagacagag tgagcagaca gaaccgaact gcacctgaac
     3841 gggttaaatc accgtcacgt ggattttcta tgggattctg aacatggcca gcacagaaga
     3901 aaaattactc acgctcctgt tatcggtgga gcatctgcaa cataccgcaa tggagcaaca
     3961 gaaagctgtt gctgattctc tggctgtggc aagggaaact cttcgcggat atcgggatat
     4021 ttctgacggt gtggcaaaac aggtcaggga tgaagtcaga aatgaggtgc agaagctgga
     4081 cgtggcagga ctgataggtg agcgcatcag ccaggatttt cagcggctgg aagagtcagt
     4141 cgcaaacatg aaggcaaacg cggatgccct gaacagggat attgcgacaa ttcacagcaa
     4201 tatcgtgcag gaatacaaca cccttaaagg gtatagctgg aaatggctgg gtggggtgtc
     4261 agtggctttt ctggttatgt tctgtgtgct cggatgggga atgaaaacaa tactcgacag
     4321 gaactacaac gccacagcgg cggtgtatca gaaagttgtg acactggaag ataaactggt
     4381 ggagaaacct gtcacgggga agagccggaa aaactgacgc gcttcgcttg tccaacgcct
     4441 cacgacccgc gaaaggattt cgcgaaccgt tcaccgtttt tttcaatatg attctgtctg
     4501 ctttggtcga taaagcagac gatagtattc cagccttcgt atatataacg gtacgtgctc
     4561 atcagtaaga gctggcggta ttgtgttatc agggggaagt tttttcagat actcacgaaa
     4621 tgcctggctt gatgccataa aatctacagt agcatcgata atatgtgggg tgttttcacc
     4681 taattttgcc atgatgataa tccttatatt atcacggttt gtgaatgata gggtgtcact
     4741 attatttatg cgttagtgaa ccagaaaaaa tcccccgttg gcataggggg atatgtaagg
     4801 agaaataact tcgcaggtgt tcttgtttgt ataacgtgaa aagactgcac gcaacttaat
     4861 aagtctgccg tacttacttt cagttttcaa cataaggttt ataacaatgt atggaatgga
     4921 agtgagcttt aaggtttcag ctctaaaaaa taaaatattt attagttggc tggttgttgt
     4981 tcttgttctg tatgtatata aacactatcg acatattatg accgggaaca ttccttaccc
     5041 cctctgcgct cgcagaggtt ttttttgtca ttaattaata atgaactgaa atgcagttcc
     5101 gatacctcca tttgcacatt atccctgaca tatggtaccg gaaccgcatt aattttatgt
     5161 ta
//
