LOCUS       NZ_CP024575             3825 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain INF274 isolate INF274 plasmid unnamed5,
            complete sequence.
ACCESSION   NZ_CP024575
VERSION     NZ_CP024575.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357268
            Assembly: GCF_002753605.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-AUG-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: INF274
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 133x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 11:26:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,692
            CDSs (total)                      :: 5,570
            Genes (coding)                    :: 5,416
            CDSs (with protein)               :: 5,416
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 51 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 15 of 154
            Pseudo Genes (multiple problems)  :: 15 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024575.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF274"
                     /isolate="INF274"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="Australia"
                     /collection_date="2013-01"
     gene            complement(121..522)
                     /locus_tag="CTI69_RS29550"
                     /old_locus_tag="CTI69_29350"
     CDS             complement(121..522)
                     /locus_tag="CTI69_RS29550"
                     /old_locus_tag="CTI69_29350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(522..713)
                     /locus_tag="CTI69_RS29555"
                     /old_locus_tag="CTI69_29355"
     CDS             complement(522..713)
                     /locus_tag="CTI69_RS29555"
                     /old_locus_tag="CTI69_29355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2869..3141)
                     /locus_tag="CTI69_RS29570"
                     /old_locus_tag="CTI69_29370"
     CDS             complement(2869..3141)
                     /locus_tag="CTI69_RS29570"
                     /old_locus_tag="CTI69_29370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004897311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_019405140.1"
                     /translation="MATDAQVKEINALIKKYPDSCSICHEVYDEDDVTYTVFGYDRKGK
                     IQVTTGCCAGMLTEPVLLGVCGCFDPEERDEIMQNHPMAKQFFTE"
                     /name="hypothetical protein"
     Typing          complement(1336..1550)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="83.562"
                     /coverage="100.0"
                     /evalue="1.62e-49"
     Typing          1531..2047
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.847"
                     /coverage="87.0"
                     /evalue="5.5e-153"
ORIGIN
        1 taattttgcg tagtgtgtgg gtatttttag tggtgaggag cgttcaggga tctgacgatt
       61 tttgggaagg ttcaggaact ccccggaatg gccggagagc aaaatggaag catggcggga
      121 tcaggatttt ttccagaatt tccaccatgg cgccggggat tcagttaatt ttgcctggtg
      181 attttcaaga aggagcatgg cctgtttcag gctgtcaatg tgcgaatcct tggacaaaag
      241 taaggtgttc aggtgttcaa tctcctgttt cagatgacga atcacctgtt cactctggtc
      301 agcagaggct gtatcacgct gtccagagac ctgtccagaa tggtgttcac ccttcggttg
      361 ttcaaaggga ccaaagacac gtaaaagctc tgaagtatct accatttttg cattgcgttc
      421 accgctacag gtggacaggt cacctgcttt aattaacctg tgcagtgttg ttctactctt
      481 acccgtcagt cgggcagcct cactaataga cactctggcc atcagttacc atcctgtaat
      541 gcatccagtc tcgaggacag tgtacggaaa agatgctccg catcatcgtg gagtttctca
      601 caaaggtccg cctcaacatc gagatcaagc tcgttaagct tctccagcag gagcagagac
      661 tgattctgga tgaatttagc catgttcagc gctgttttct gttgtttcgt cataagggcc
      721 ctcagagcat cagtgctcag tgtaaaccgg taagggggtc ggatgtacga atggttcaga
      781 gaacggatct gaggttatcc tgtagttatt ttgtagttta tctgtagtca tcgcactaaa
      841 acgcaggatt aaatcatcaa cttttgtagt cttcctgtag tttacctgta gtctggaaaa
      901 ataatccgtt catgcaagca tgaacgaagc agttaaagac gctttttcgt cgccgatgat
      961 ccttaacctg cgcagatcac ccagccagaa tgataatttt ttgcccctta aatggtcata
     1021 cacctaacgc agccgcgatt tttatcgcaa ctgcatgaat taaatgaaaa aaatggagtt
     1081 atttcgtgta ttagcgtaat acgcaacgta ttacaatcat ctgtaacaac atgaaaataa
     1141 acaaataaca tgtaatacca gcgtattaca tgtacgctgg ccagcgaaaa agctataacg
     1201 cttcccggcc gacaggcggg ccagaatcca ccggggcgtg caggttctct tcagtaacat
     1261 gagccattaa gtcaaaaagg tcatattcgc cgggaaacag agcatcagtc gcatccggtt
     1321 gttcatcata ctcagatggc atggtgtgat atccagtagc tgaggggtgt tagcgggttt
     1381 cggggcgcag ccctgaacca gtcacgtagc gctagcggag tgcatactgg ctaaatattt
     1441 taaaccgaag tggtgatttg ccaaagtgct tcatgtggca ggagtgaaat gaggctttcc
     1501 ggcgtcagga gaagatgctg gttcaggtga tattccgctt cctcgctcac tgactcgctg
     1561 cgctcggtcg ttcggctgcg gcgagcggtg caggcttact aatggggcgg aaatttattt
     1621 cctggtggat gccaggaaga aacttaacag ggatacgata aggccgcggc aaagccgttt
     1681 ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg
     1741 cgaaacccga caggactata aagataccag gcgtttcccc ctggtggctc cctcttgcgc
     1801 tctcctgttc ccgtcctgcg gcatccgtgt agtggtggaa gctttaccta aatcaccacg
     1861 ccccgttccg tttagacagt tcgctccaag ctgggctgta tgcaagaacc ccccgttcag
     1921 cccgaccgct gcgccttttc cggtaactat cgtcttgagt ccaacccgga aagacacgac
     1981 ttaacgccac tggcagcggc cactggtaaa caggatgtgc gagagattta cgaagagttc
     2041 ttgaagtggt ggccagactg aggctacact aaaaggacag atttggtgac gtctcgcaca
     2101 agactgttac cacggttcag aaacttgccg gtttactgaa ccttcgaaaa accaccctgc
     2161 aaggtggttt tttcgtttac atggcaagag actacgacgt ccaacgtcag atctcaagaa
     2221 attatttaaa aataaatagt taaccaatat ccttgtgata tttattatgt ttcctttata
     2281 ctcaaataac atcaatattc aggattggaa cactcaaaca agttaataca acgatgttct
     2341 aggttcatat ccctgagact taaagtctga ctagtatatt tccgaacaag atcactatca
     2401 ttccgtactt ttttaaatag gtttatttaa tgccatacat atacgcaata atactaacaa
     2461 cttgttttaa cacgaataat attgcaagtt gttcttcaga attagcaaat ttgggaatga
     2521 aagacaatgt tgttaacacc ttcattctga atgaagaatg cattgaattc aaagataaaa
     2581 tcattgcaaa caaagacatt tatcataata tatccgggaa aaacataata tctgctgaat
     2641 gtgtacccgt atctatcccc aaatatattt tagacaatta cagcaaacaa caacgataat
     2701 gaatcccccc tctgcaagca gcggggtatc aacaccaaag atcaaataca gagtagttcg
     2761 ctccaaggga cgggaaatat tgcctttaga gatttaccat aacgtctcat ttcacgcaga
     2821 tatgaaaaag cccgcacaag gcgggcttca gactcaacag atacggaatc actctgtgaa
     2881 aaactgcttt gccatggggt gattctgcat gatctcgtca cgttcttccg ggtcaaagca
     2941 cccacagaca cccagcagaa ccggctctgt cagcattccg gcacagcatc ccgtggtgac
     3001 ctgtatttta cctttccggt catacccaaa aacggtgtag gtcacatcat cctcatcata
     3061 gacttcatga cagatggagc atgagtccgg gtattttttg atgagtgcat taatttcttt
     3121 tacctgagcg tcagtagcca aaacatctcc ctgagtcact gattatcaca atccgctgac
     3181 tctaacacag actcaggcat ccgccgttga tttaccataa cgtcccatta cgcgcaccgc
     3241 gaaaacggcc tcagggcgtt cctgacggga aaccggtaaa acaggcaaca aaccacccga
     3301 aaatccgcca gaaacgcgct ggctctgttt ttacggcatg catgagtatg cacgcaagtg
     3361 catagcgcgg aaattcacgc cattcagcca gggtaagtcc tgtttccggc gttcttccta
     3421 tccgaaaacg gtgaacgggg acgcaattta ttgcgtggcc gtgaagcgtt gccggcgcag
     3481 ccggaaaacc ggttttcagc acgatcagga atcgggttcg tcgacggccg gcaatcacgt
     3541 tatggcaagt cgccggacga ctcaccagac aattcaccgg cgattcgact cgtgacttga
     3601 ctctagagtc gccatggtca aaaagccacc aacccggcaa gtcgactcta gagtcaagtc
     3661 atcataacgt actgattata tggcgtgatt cacatatgaa tcgtgggcaa gtcatcggcg
     3721 attcgccata gagtcgtcca cgactcggat tcaccttaca aaaaataaaa ttgcgtaatt
     3781 taaaacagat aactgattga aaataaaggt ctatctgcaa aattt
//
